pycom09g10020

negative regulation of chemokine (C-C motif) ligand 5 production

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002439
Physical Location & Seq
Forward (+)
8090025 .. 8090595
571 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g10020.2

Sequence Viewer

Length: 387 bp
ATGTCGTTGCACGAGCGAGCCGTGATGAACCTATTCTCGCAACTCGCCCTCTCCTACAACGGCGCCGCTTTGGGCGTGGTCCTAGCGTGCGCCGCCGCGCGTACCGTCATCAAGTTCGTCTACACCTCCTCAGCACTGCTCAAGCTCCGTAATGCTCCCTACGTCAAGGTCTCCGGACTCCGCGCAATCCTCGCCCCTGACGAATCTCAGCCCTCAGATGCAAAGCTCGTCGTCGTTCAAAGAACTCAAACTGCGCGGTTGGGCCATATTTCTGTAAAAAGAAATAGGCCCAGGAAAGTCCCCGGGCCCACTATCTGCAGAGATGCAGGCCTGGAAACAGGTAGCCTCCGTGTGCTTGGAGTCTTATCGCCCCGTGCGATGGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000187 GO:0000266 GO:0001558 GO:0001817 GO:0001818 GO:0001932 GO:0001934 GO:0001959 GO:0001960 GO:0002682 GO:0002683 GO:0002697 GO:0002698 GO:0002831 GO:0002832 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005777 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006839 GO:0006919 GO:0006950 GO:0006996 GO:0007005 GO:0007006 GO:0007154 GO:0007165 GO:0007254 GO:0007257 GO:0007275 GO:0007399 GO:0008150 GO:0008152 GO:0008270 GO:0009507 GO:0009526 GO:0009536 GO:0009893 GO:0009894 GO:0009896 GO:0009941 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010033 GO:0010506 GO:0010508 GO:0010562 GO:0010604 GO:0010635 GO:0010637 GO:0010638 GO:0010639 GO:0010646 GO:0010647 GO:0010648 GO:0010720 GO:0010821 GO:0010822 GO:0010823 GO:0010941 GO:0010942 GO:0010950 GO:0010952 GO:0010975 GO:0010976 GO:0014070 GO:0016020 GO:0016021 GO:0016043 GO:0016310 GO:0016567 GO:0016740 GO:0016925 GO:0018193 GO:0018205 GO:0019220 GO:0019222 GO:0019538 GO:0019787 GO:0019789 GO:0019867 GO:0019899 GO:0022008 GO:0022603 GO:0022604 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0023057 GO:0030154 GO:0030162 GO:0030307 GO:0030308 GO:0030424 GO:0031090 GO:0031098 GO:0031224 GO:0031300 GO:0031301 GO:0031306 GO:0031307 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031344 GO:0031346 GO:0031347 GO:0031348 GO:0031399 GO:0031401 GO:0031625 GO:0031647 GO:0031648 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0032101 GO:0032102 GO:0032147 GO:0032268 GO:0032270 GO:0032446 GO:0032501 GO:0032502 GO:0032592 GO:0032642 GO:0032682 GO:0032872 GO:0032874 GO:0032879 GO:0033043 GO:0033233 GO:0033235 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0036477 GO:0040008 GO:0042221 GO:0042325 GO:0042327 GO:0042391 GO:0042579 GO:0042802 GO:0042981 GO:0042995 GO:0043005 GO:0043025 GO:0043065 GO:0043067 GO:0043068 GO:0043085 GO:0043122 GO:0043123 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043280 GO:0043281 GO:0043330 GO:0043331 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043506 GO:0043507 GO:0043549 GO:0043900 GO:0043901 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044297 GO:0044389 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045088 GO:0045595 GO:0045597 GO:0045664 GO:0045666 GO:0045824 GO:0045859 GO:0045860 GO:0045862 GO:0045926 GO:0045927 GO:0045937 GO:0046328 GO:0046330 GO:0046872 GO:0046902 GO:0046914 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048638 GO:0048639 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050687 GO:0050688 GO:0050689 GO:0050691 GO:0050767 GO:0050769 GO:0050776 GO:0050777 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050821 GO:0050896 GO:0051049 GO:0051093 GO:0051094 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051239 GO:0051240 GO:0051241 GO:0051246 GO:0051247 GO:0051336 GO:0051338 GO:0051345 GO:0051347 GO:0051403 GO:0051640 GO:0051641 GO:0051646 GO:0051716 GO:0051881 GO:0051896 GO:0051898 GO:0051960 GO:0051962 GO:0052547 GO:0052548 GO:0060255 GO:0060284 GO:0060338 GO:0060339 GO:0060759 GO:0060761 GO:0061024 GO:0065007 GO:0065008 GO:0065009 GO:0070302 GO:0070304 GO:0070647 GO:0070887 GO:0071310 GO:0071359 GO:0071360 GO:0071407 GO:0071649 GO:0071650 GO:0071704 GO:0071840 GO:0071900 GO:0071902 GO:0080090 GO:0080134 GO:0080135 GO:0090140 GO:0090141 GO:0090559 GO:0097458 GO:0098573 GO:0098588 GO:0098780 GO:0098805 GO:0120025 GO:0120035 GO:0140096 GO:1901028 GO:1901564 GO:1901698 GO:1901699 GO:1902531 GO:1902532 GO:1902533 GO:1903146 GO:1903320 GO:1903322 GO:1903599 GO:1903859 GO:1903861 GO:1904923 GO:1904925 GO:2000026 GO:2000116 GO:2001056 GO:2001233
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GIDE zf-C3HC4_3

Protein Analysis

129

Amino Acids

13.68

Weight (kDa)

10.94

Isoelectric Point (pI)

38.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 62
AccI GTMKAC 1 cut(s) 120
AccII CGCG 4 cut(s) 98, 100, 183, 256
AccIII TCCGGA 1 cut(s) 173
AciI CCGC 5 cut(s) 66, 93, 96, 181, 256
AcyI GRCGYC 1 cut(s) 63
AfaI GTAC 1 cut(s) 103
AfiI CCNNNNNNNGG 1 cut(s) 379
AgsI TTSAA 1 cut(s) 239
AjnI CCWGG 2 cut(s) 290, 330
AluBI AGCT 2 cut(s) 145, 226
AluI AGCT 2 cut(s) 145, 226
Alw26I GTCTC 1 cut(s) 175
Ama87I CYCGRG 1 cut(s) 302
Aor13HI TCCGGA 1 cut(s) 173
AoxI GGCC 4 cut(s) 262, 287, 305, 328
ApaI GGGCCC 1 cut(s) 309
Asp700I GAANNNNTTC 1 cut(s) 32
AspLEI GCGC 5 cut(s) 65, 92, 100, 185, 256
AspS9I GGNCC 5 cut(s) 79, 262, 288, 305, 306
AsuC2I CCSGG 2 cut(s) 303, 304
AvaI CYCGRG 1 cut(s) 302
AvaII GGWCC 1 cut(s) 79
BaeGI GKGCMC 1 cut(s) 309
BanI GGYRCC 1 cut(s) 62
BanII GRGCYC 1 cut(s) 309
BauI CACGAG 1 cut(s) 11
BbvCI CCTCAGC 1 cut(s) 130
BccI CCATC 1 cut(s) 373
BceAI ACGGC 2 cut(s) 5, 76
BciT130I CCWGG 2 cut(s) 292, 332
BcnI CCSGG 2 cut(s) 303, 304
BcoDI GTCTC 1 cut(s) 175
BfaI CTAG 1 cut(s) 83
BfmI CTRYAG 1 cut(s) 316
BfoI RGCGCY 1 cut(s) 66
BisI GCNGC 3 cut(s) 66, 93, 96
BlsI GCNGC 3 cut(s) 67, 94, 97
Bme1390I CCNGG 4 cut(s) 292, 303, 304, 332
Bme18I GGWCC 1 cut(s) 79
BmeT110I CYCGRG 1 cut(s) 302
BmgT120I GGNCC 5 cut(s) 79, 262, 288, 305, 306
BmiI GGNNCC 2 cut(s) 64, 307
BmrFI CCNGG 4 cut(s) 292, 303, 304, 332
BmsI GCATC 2 cut(s) 208, 313
Bpu10I CCTNAGC 1 cut(s) 130
BpuEI CTTGAG 1 cut(s) 125
BpuMI CCSGG 2 cut(s) 303, 304
BsaHI GRCGYC 1 cut(s) 63
BsaI GGTCTC 1 cut(s) 175
BsaJI CCNNGG 3 cut(s) 290, 301, 302
BsaWI WCCGGW 1 cut(s) 173
Bsc4I CCNNNNNNNGG 1 cut(s) 379
BseAI TCCGGA 1 cut(s) 173
BseBI CCWGG 2 cut(s) 292, 332
BseDI CCNNGG 3 cut(s) 290, 301, 302
BseLI CCNNNNNNNGG 1 cut(s) 379
BseMII CTCAG 3 cut(s) 144, 221, 228
BseRI GAGGAG 1 cut(s) 118
BseSI GKGCMC 1 cut(s) 309
Bsh1236I CGCG 4 cut(s) 98, 100, 183, 256
BshFI GGCC 4 cut(s) 264, 289, 307, 330
BshNI GGYRCC 1 cut(s) 62
BsiHKCI CYCGRG 1 cut(s) 302
BsiSI CCGG 2 cut(s) 174, 303
BslFI GGGAC 1 cut(s) 284
BslI CCNNNNNNNGG 1 cut(s) 379
BsmAI GTCTC 1 cut(s) 175
BsmFI GGGAC 1 cut(s) 284
BsnI GGCC 4 cut(s) 264, 289, 307, 330
Bso31I GGTCTC 1 cut(s) 175
BsoBI CYCGRG 1 cut(s) 302
Bsp120I GGGCCC 1 cut(s) 305
Bsp1286I GDGCHC 1 cut(s) 309
Bsp13I TCCGGA 1 cut(s) 173
BspACI CCGC 5 cut(s) 66, 93, 96, 181, 256
BspANI GGCC 4 cut(s) 264, 289, 307, 330
BspCNI CTCAG 3 cut(s) 143, 220, 227
BspEI TCCGGA 1 cut(s) 173
BspFNI CGCG 4 cut(s) 98, 100, 183, 256
BspLI GGNNCC 2 cut(s) 64, 307
BspMAI CTGCAG 1 cut(s) 320
BspT107I GGYRCC 1 cut(s) 62
BspTNI GGTCTC 1 cut(s) 175
BssECI CCNNGG 3 cut(s) 290, 301, 302
BssNI GRCGYC 1 cut(s) 63
BssSI CACGAG 1 cut(s) 11
Bst2BI CACGAG 1 cut(s) 11
Bst2UI CCWGG 2 cut(s) 292, 332
Bst4CI ACNGT 1 cut(s) 106
BstACI GRCGYC 1 cut(s) 63
BstC8I GCNNGC 3 cut(s) 18, 88, 328
BstDEI CTNAG 3 cut(s) 130, 207, 214
BstFNI CGCG 4 cut(s) 98, 100, 183, 256
BstH2I RGCGCY 1 cut(s) 66
BstHHI GCGC 5 cut(s) 65, 92, 100, 185, 256
BstMAI GTCTC 1 cut(s) 175
BstMWI GCNNNNNNNGC 2 cut(s) 92, 191
BstNI CCWGG 2 cut(s) 292, 332
BstSCI CCNGG 4 cut(s) 290, 301, 302, 330
BstSFI CTRYAG 1 cut(s) 316
BstSLI GKGCMC 1 cut(s) 309
BstUI CGCG 4 cut(s) 98, 100, 183, 256
BsuRI GGCC 4 cut(s) 264, 289, 307, 330
BtsI GCAGTG 1 cut(s) 134
BtsIMutI CAGTG 1 cut(s) 134
Cac8I GCNNGC 3 cut(s) 18, 88, 328
CfoI GCGC 5 cut(s) 65, 92, 100, 185, 256
Cfr13I GGNCC 5 cut(s) 79, 262, 288, 305, 306
Cfr9I CCCGGG 1 cut(s) 302
Csp6I GTAC 1 cut(s) 102
CviQI GTAC 1 cut(s) 102
DdeI CTNAG 3 cut(s) 130, 207, 214
DinI GGCGCC 1 cut(s) 64
Eco147I AGGCCT 1 cut(s) 330
Eco24I GRGCYC 1 cut(s) 309
Eco31I GGTCTC 1 cut(s) 175
Eco47I GGWCC 1 cut(s) 79
Eco88I CYCGRG 1 cut(s) 302
EcoRII CCWGG 2 cut(s) 290, 330
EcoT38I GRGCYC 1 cut(s) 309
EgeI GGCGCC 1 cut(s) 64
EheI GGCGCC 1 cut(s) 64
FaiI YATR 1 cut(s) 267
FaqI GGGAC 1 cut(s) 284
FblI GTMKAC 1 cut(s) 120
Fnu4HI GCNGC 3 cut(s) 66, 93, 96
FriOI GRGCYC 1 cut(s) 309
Fsp4HI GCNGC 3 cut(s) 66, 93, 96
FspBI CTAG 1 cut(s) 83
GlaI GCGC 5 cut(s) 64, 91, 99, 184, 255
GluI GCNGC 3 cut(s) 66, 93, 96
HaeII RGCGCY 1 cut(s) 66
HaeIII GGCC 4 cut(s) 264, 289, 307, 330
HapII CCGG 2 cut(s) 174, 303
HhaI GCGC 5 cut(s) 65, 92, 100, 185, 256
Hin1I GRCGYC 1 cut(s) 63
Hin6I GCGC 5 cut(s) 63, 90, 98, 183, 254
HinP1I GCGC 5 cut(s) 63, 90, 98, 183, 254
HinfI GANTC 3 cut(s) 177, 203, 360
HpaII CCGG 2 cut(s) 174, 303
Hpy166II GTNNAC 1 cut(s) 121
Hpy188I TCNGA 1 cut(s) 217
Hpy188III TCNNGA 1 cut(s) 174
Hpy8I GTNNAC 1 cut(s) 121
Hpy99I CGWCG 2 cut(s) 233, 236
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4IV ACGT 1 cut(s) 162
HpyCH4V TGCA 4 cut(s) 10, 221, 318, 326
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 191
HpyF3I CTNAG 3 cut(s) 130, 207, 214
HpySE526I ACGT 1 cut(s) 162
Hsp92I GRCGYC 1 cut(s) 63
HspAI GCGC 5 cut(s) 63, 90, 98, 183, 254
KasI GGCGCC 1 cut(s) 62
Kpn2I TCCGGA 1 cut(s) 173
LmnI GCTCC 2 cut(s) 150, 160
LpnPI CCDG 9 cut(s) 187, 210, 277, 304, 312, 316, 317, 324, 344
LweI GCATC 2 cut(s) 208, 313
MaeI CTAG 1 cut(s) 83
MaeII ACGT 1 cut(s) 162
MhlI GDGCHC 1 cut(s) 309
Mly113I GGCGCC 1 cut(s) 63
MlyI GAGTC 2 cut(s) 171, 369
MnlI CCTC 6 cut(s) 59, 136, 139, 200, 223, 356
MroI TCCGGA 1 cut(s) 173
MroXI GAANNNNTTC 1 cut(s) 32
MspI CCGG 2 cut(s) 174, 303
MspR9I CCNGG 4 cut(s) 292, 303, 304, 332
MvaI CCWGG 2 cut(s) 292, 332
MvnI CGCG 4 cut(s) 98, 100, 183, 256
MwoI GCNNNNNNNGC 2 cut(s) 92, 191
NarI GGCGCC 1 cut(s) 63
NciI CCSGG 2 cut(s) 303, 304
NlaIV GGNNCC 2 cut(s) 64, 307
PceI AGGCCT 1 cut(s) 330
PcsI WCGNNNNNNNCGW 3 cut(s) 18, 198, 374
PdmI GAANNNNTTC 1 cut(s) 32
PfeI GAWTC 1 cut(s) 203
PkrI GCNGC 3 cut(s) 67, 94, 97
PleI GAGTC 2 cut(s) 171, 368
PluTI GGCGCC 1 cut(s) 66
PpsI GAGTC 2 cut(s) 171, 368
Psp6I CCWGG 2 cut(s) 290, 330
PspGI CCWGG 2 cut(s) 290, 330
PspN4I GGNNCC 2 cut(s) 64, 307
PspOMI GGGCCC 1 cut(s) 305
PspPI GGNCC 5 cut(s) 79, 262, 288, 305, 306
PstI CTGCAG 1 cut(s) 320
RsaI GTAC 1 cut(s) 103
RsaNI GTAC 1 cut(s) 102
SatI GCNGC 3 cut(s) 66, 93, 96
Sau96I GGNCC 5 cut(s) 79, 262, 288, 305, 306
SchI GAGTC 2 cut(s) 171, 369
ScrFI CCNGG 4 cut(s) 292, 303, 304, 332
SduI GDGCHC 1 cut(s) 309
SetI ASST 7 cut(s) 33, 128, 147, 165, 171, 228, 343
SfaNI GCATC 2 cut(s) 208, 313
SfcI CTRYAG 1 cut(s) 316
SfoI GGCGCC 1 cut(s) 64
SinI GGWCC 1 cut(s) 79
SmaI CCCGGG 1 cut(s) 304
SmlI CTYRAG 1 cut(s) 140
SmoI CTYRAG 1 cut(s) 140
SseBI AGGCCT 1 cut(s) 330
SsiI CCGC 5 cut(s) 66, 93, 96, 181, 256
SspDI GGCGCC 1 cut(s) 62
SspMI CTAG 1 cut(s) 83
StuI AGGCCT 1 cut(s) 330
StyD4I CCNGG 4 cut(s) 290, 301, 302, 330
TaaI ACNGT 1 cut(s) 106
TaiI ACGT 1 cut(s) 165
TauI GCSGC 3 cut(s) 68, 95, 98
TfiI GAWTC 1 cut(s) 203
TscAI CASTG 1 cut(s) 141
TspDTI ATGAA 1 cut(s) 41
TspGWI ACGGA 2 cut(s) 137, 338
TspMI CCCGGG 1 cut(s) 302
TspRI CASTG 1 cut(s) 141
VpaK11BI GGWCC 1 cut(s) 79
XmaI CCCGGG 1 cut(s) 302
XmiI GTMKAC 1 cut(s) 120
XmnI GAANNNNTTC 1 cut(s) 32
XspI CTAG 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.