pycom09g09990

Mitochondrial ubiquitin ligase activator of NFKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002439
Physical Location & Seq
Forward (+)
8079772 .. 8082548
2777 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g09990.1

Sequence Viewer

Length: 1137 bp
ATGTCGTCGCCAGTGCCAGACAATGTCTTCGTCTCCCAAGTCTCCCGATTCGTCCTCTCCTGGGACGGCGCCGTTCTGGGCGTCTCCCTAGCTTGCGCCGCCGTGCACTCCGTCCTCAATTTCACCTCCACCTCCTCATCCCTAAGCAAGCTCCGTGATGCCCCCGCCGTCAAAGTCTCCGATCTCCGCTCAATCCTCCCCCTCGATCAATCCGACAAGCTCGTCGTTGTACGCGGCACCGTCGAAGCCAAGTCCGCCGTCGACGACACGTGGACGAGCCTCAAGTCCGGCGTACTCATCTCTAAAGAAACAAATCACAGAGCCGTCGTCGTTGACAGTACTAAAACGTGTATAGTTCAGGAGTGGAAGGGCTTAATCGGATGGTCGTCTGATTTGAGAGCGATAATTTGGAGGTATTGGAGAGAGCACGAATCCAATTTGGTCAGAAGGGTTCCTTTCGTTCTTGTTGATGGTGATCAATGGCCGATTCGGGATATTGTGGTTGTGAATGTGGAAGGATCAAGGCATCCTTTACCTCTTACGATAGCTTATCATCATTTGCAACCCGTACATGCTTCTCCCTATTCTTTCTTGGAAGCACTTTTTGGCACTAACTACCCTGTTGCTTTGCTCAACGTTGAGAAGATTCTTCCGGTGGGAAAGGAGATTAGTGCTGTAGGTCTCTGCAGTTTGAGAAATGGAGTCCCGGAAATCAAGTCCTGCAAGGATCTTCCCTATTTTCTATGTCTAAGGATGAGATGGTTGCAGATCTTGCATCACGTGCAAAAATGCTGTTCTGGGTTGGGATTGTTCTTGGTTCAATGTCAATTGGAGTCCTTGGTTATGCTGAATTGGAATAGATGGAAAGTGGAGAGGCAACAACGGCAGTTACAGCAATCAAGCCGGGATGCTGACAGCAATGCTGAAGCTCAAATTGAAGAAGAGGATGCAGGAGATGTTCCAGAGGGGCAGTTGTGTGTCATATGTCTGACGAGGAGAAGGCGGTCAGCTTTCATTCCTTGCGGGCATCGAGTTTGTTGCCACCCGTGCAGCATATCAATCGAACGGGATGTAGCACCAAAGTGTCCTGTTTGTCGGCAGGAGATCCGCACTTCGCTGCGAATATATGATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000266 GO:0001558 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005777 GO:0006464 GO:0006807 GO:0006919 GO:0006996 GO:0007005 GO:0008150 GO:0008152 GO:0009507 GO:0009526 GO:0009536 GO:0009893 GO:0009941 GO:0009966 GO:0009967 GO:0009987 GO:0010604 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0010950 GO:0010952 GO:0016020 GO:0016021 GO:0016043 GO:0016567 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019867 GO:0023051 GO:0023056 GO:0030162 GO:0030308 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031306 GO:0031307 GO:0031323 GO:0031325 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0032268 GO:0032270 GO:0032446 GO:0032592 GO:0036211 GO:0040008 GO:0042579 GO:0042802 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043085 GO:0043122 GO:0043123 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043280 GO:0043281 GO:0043412 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045862 GO:0045926 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0051128 GO:0051171 GO:0051173 GO:0051179 GO:0051246 GO:0051247 GO:0051336 GO:0051345 GO:0051640 GO:0051641 GO:0051646 GO:0052547 GO:0052548 GO:0060255 GO:0065007 GO:0065009 GO:0070647 GO:0071704 GO:0071840 GO:0080090 GO:0098573 GO:0098588 GO:0098805 GO:0140096 GO:1901564 GO:1902531 GO:1902533 GO:2000116 GO:2001056
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GIDE zf-C3HC4_3

Protein Analysis

379

Amino Acids

42.4

Weight (kDa)

8.3

Isoelectric Point (pI)

57.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIDE PF12483 134 - 250 6.5e-23 E3 Ubiquitin ligase
zf-C3HC4_3 PF13920 323 - 371 3.9e-13 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 221
AccB1I GGYRCC 2 cut(s) 68, 236
AccBSI CCGCTC 1 cut(s) 189
AccI GTMKAC 1 cut(s) 261
AccII CGCG 1 cut(s) 234
AciI CCGC 8 cut(s) 99, 165, 187, 234, 255, 1003, 1023, 1108
AclI AACGTT 1 cut(s) 636
AclWI GGATC 3 cut(s) 526, 735, 1099
AcoI YGGCCR 1 cut(s) 482
AcuI CTGAAG 1 cut(s) 945
AcvI CACGTG 2 cut(s) 270, 781
AcyI GRCGYC 2 cut(s) 69, 81
AfaI GTAC 4 cut(s) 231, 294, 340, 570
AfiI CCNNNNNNNGG 1 cut(s) 61
AflIII ACRYGT 2 cut(s) 267, 347
AgsI TTSAA 2 cut(s) 821, 938
AjnI CCWGG 1 cut(s) 59
AjuI GAANNNNNNNTTGG 2 cut(s) 588, 620
AleI CACNNNNGTG 1 cut(s) 1081
AluBI AGCT 6 cut(s) 92, 151, 220, 548, 929, 1010
AluI AGCT 6 cut(s) 92, 151, 220, 548, 929, 1010
Alw21I GWGCWC 2 cut(s) 108, 429
Alw26I GTCTC 5 cut(s) 37, 46, 88, 181, 686
Alw44I GTGCAC 1 cut(s) 104
AlwI GGATC 3 cut(s) 526, 735, 1099
AoxI GGCC 1 cut(s) 482
ApaLI GTGCAC 1 cut(s) 104
ApeKI GCWGC 2 cut(s) 1050, 1117
ArsI GACNNNNNNTTYG 2 cut(s) 11, 43
AspLEI GCGC 2 cut(s) 71, 98
AsuC2I CCSGG 2 cut(s) 707, 905
AsuHPI GGTGA 2 cut(s) 115, 485
BaeGI GKGCMC 1 cut(s) 108
BanI GGYRCC 2 cut(s) 68, 236
BbrPI CACGTG 2 cut(s) 270, 781
BbsI GAAGAC 1 cut(s) 19
Bbv12I GWGCWC 2 cut(s) 108, 429
BbvI GCAGC 2 cut(s) 1062, 1104
BccI CCATC 4 cut(s) 375, 464, 753, 855
BceAI ACGGC 7 cut(s) 56, 82, 86, 152, 242, 308, 899
BcgI CGANNNNNNTGC 4 cut(s) 1020, 1042, 1054, 1076
BciT130I CCWGG 1 cut(s) 61
BclI TGATCA 1 cut(s) 475
BcnI CCSGG 2 cut(s) 707, 905
BcoDI GTCTC 5 cut(s) 37, 46, 88, 181, 686
BfaI CTAG 1 cut(s) 89
BfmI CTRYAG 2 cut(s) 675, 685
BfoI RGCGCY 1 cut(s) 72
BglII AGATCT 1 cut(s) 768
BisI GCNGC 4 cut(s) 99, 235, 1051, 1118
BlsI GCNGC 4 cut(s) 100, 236, 1052, 1119
BmcAI AGTACT 1 cut(s) 340
Bme1390I CCNGG 3 cut(s) 61, 707, 905
BmiI GGNNCC 3 cut(s) 70, 238, 453
BmrFI CCNGG 3 cut(s) 61, 707, 905
BmsI GCATC 6 cut(s) 148, 535, 784, 898, 937, 1036
BpiI GAAGAC 1 cut(s) 19
Bpu10I CCTNAGC 1 cut(s) 143
BpuEI CTTGAG 1 cut(s) 266
BpuMI CCSGG 2 cut(s) 707, 905
BsaAI YACGTR 2 cut(s) 270, 781
BsaBI GATNNNNATC 1 cut(s) 474
BsaHI GRCGYC 2 cut(s) 69, 81
BsaI GGTCTC 1 cut(s) 686
BsaJI CCNNGG 2 cut(s) 60, 837
BsaWI WCCGGW 1 cut(s) 652
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse1I ACTGG 1 cut(s) 11
Bse3DI GCAATG 1 cut(s) 925
Bse8I GATNNNNATC 1 cut(s) 474
BseBI CCWGG 1 cut(s) 61
BseDI CCNNGG 2 cut(s) 60, 837
BseGI GGATG 7 cut(s) 137, 386, 526, 759, 913, 952, 1075
BseJI GATNNNNATC 1 cut(s) 474
BseLI CCNNNNNNNGG 1 cut(s) 61
BseMI GCAATG 1 cut(s) 925
BseNI ACTGG 1 cut(s) 11
BseRI GAGGAG 2 cut(s) 124, 1009
BseSI GKGCMC 1 cut(s) 108
BseXI GCAGC 2 cut(s) 1062, 1104
BsgI GTGCAG 1 cut(s) 1069
Bsh1236I CGCG 1 cut(s) 234
BshFI GGCC 1 cut(s) 484
BshNI GGYRCC 2 cut(s) 68, 236
BsiHKAI GWGCWC 2 cut(s) 108, 429
BsiSI CCGG 4 cut(s) 288, 653, 707, 904
BslFI GGGAC 2 cut(s) 77, 689
BslI CCNNNNNNNGG 1 cut(s) 61
BsmAI GTCTC 5 cut(s) 37, 46, 88, 181, 686
BsmBI CGTCTC 2 cut(s) 37, 88
BsmFI GGGAC 2 cut(s) 77, 689
BsnI GGCC 1 cut(s) 484
Bso31I GGTCTC 1 cut(s) 686
Bsp1286I GDGCHC 2 cut(s) 108, 429
Bsp143I GATC 7 cut(s) 181, 205, 475, 518, 727, 768, 1104
BspACI CCGC 8 cut(s) 99, 165, 187, 234, 255, 1003, 1023, 1108
BspANI GGCC 1 cut(s) 484
BspFNI CGCG 1 cut(s) 234
BspLI GGNNCC 3 cut(s) 70, 238, 453
BspMAI CTGCAG 1 cut(s) 689
BspPI GGATC 3 cut(s) 526, 735, 1099
BspT107I GGYRCC 2 cut(s) 68, 236
BspTNI GGTCTC 1 cut(s) 686
BsrBI CCGCTC 1 cut(s) 189
BsrDI GCAATG 1 cut(s) 925
BsrI ACTGG 1 cut(s) 11
BssECI CCNNGG 2 cut(s) 60, 837
BssMI GATC 7 cut(s) 181, 205, 475, 518, 727, 768, 1104
BssNI GRCGYC 2 cut(s) 69, 81
BssT1I CCWWGG 1 cut(s) 837
Bst2UI CCWGG 1 cut(s) 61
Bst4CI ACNGT 2 cut(s) 241, 338
Bst6I CTCTTC 1 cut(s) 936
BstACI GRCGYC 2 cut(s) 69, 81
BstAPI GCANNNNNTGC 2 cut(s) 772, 781
BstBAI YACGTR 2 cut(s) 270, 781
BstC8I GCNNGC 3 cut(s) 94, 149, 1025
BstDEI CTNAG 2 cut(s) 143, 749
BstF5I GGATG 7 cut(s) 137, 386, 526, 759, 913, 952, 1075
BstFNI CGCG 1 cut(s) 234
BstH2I RGCGCY 1 cut(s) 72
BstHHI GCGC 2 cut(s) 71, 98
BstKTI GATC 7 cut(s) 184, 208, 478, 521, 730, 771, 1107
BstMAI GTCTC 5 cut(s) 37, 46, 88, 181, 686
BstMBI GATC 7 cut(s) 181, 205, 475, 518, 727, 768, 1104
BstMWI GCNNNNNNNGC 7 cut(s) 98, 254, 772, 781, 883, 892, 1047
BstNI CCWGG 1 cut(s) 61
BstNSI RCATGY 1 cut(s) 575
BstSCI CCNGG 3 cut(s) 59, 705, 903
BstSFI CTRYAG 2 cut(s) 675, 685
BstSLI GKGCMC 1 cut(s) 108
BstUI CGCG 1 cut(s) 234
BstV1I GCAGC 2 cut(s) 1062, 1104
BstV2I GAAGAC 1 cut(s) 19
BstX2I RGATCY 3 cut(s) 727, 768, 1104
BstYI RGATCY 3 cut(s) 727, 768, 1104
BsuRI GGCC 1 cut(s) 484
BtsCI GGATG 7 cut(s) 137, 386, 526, 759, 913, 952, 1075
BtsIMutI CAGTG 1 cut(s) 18
Cac8I GCNNGC 3 cut(s) 94, 149, 1025
CfoI GCGC 2 cut(s) 71, 98
CseI GACGC 1 cut(s) 70
Csp6I GTAC 4 cut(s) 230, 293, 339, 569
CviAII CATG 1 cut(s) 572
CviQI GTAC 4 cut(s) 230, 293, 339, 569
DdeI CTNAG 2 cut(s) 143, 749
DinI GGCGCC 1 cut(s) 70
DpnI GATC 7 cut(s) 183, 207, 477, 520, 729, 770, 1106
DpnII GATC 7 cut(s) 181, 205, 475, 518, 727, 768, 1104
DrdI GACNNNNNNGTC 1 cut(s) 221
DseDI GACNNNNNNGTC 1 cut(s) 221
EaeI YGGCCR 1 cut(s) 482
Eam1104I CTCTTC 1 cut(s) 936
EarI CTCTTC 1 cut(s) 936
EciI GGCGGA 1 cut(s) 244
Eco130I CCWWGG 1 cut(s) 837
Eco31I GGTCTC 1 cut(s) 686
Eco57I CTGAAG 1 cut(s) 945
Eco72I CACGTG 2 cut(s) 270, 781
EcoRII CCWGG 1 cut(s) 59
EcoT14I CCWWGG 1 cut(s) 837
EgeI GGCGCC 1 cut(s) 70
EheI GGCGCC 1 cut(s) 70
ErhI CCWWGG 1 cut(s) 837
Esp3I CGTCTC 2 cut(s) 37, 88
FaeI CATG 1 cut(s) 575
FaiI YATR 9 cut(s) 353, 573, 745, 845, 983, 985, 1055, 1126, 1128
FalI AAGNNNNNCTT 4 cut(s) 439, 471, 514, 546
FaqI GGGAC 2 cut(s) 77, 689
FatI CATG 1 cut(s) 571
FauI CCCGC 2 cut(s) 172, 1016
FauNDI CATATG 1 cut(s) 983
FbaI TGATCA 1 cut(s) 475
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 4 cut(s) 99, 235, 1051, 1118
FokI GGATG 7 cut(s) 124, 393, 513, 766, 920, 959, 1082
Fsp4HI GCNGC 4 cut(s) 99, 235, 1051, 1118
FspBI CTAG 1 cut(s) 89
GlaI GCGC 2 cut(s) 70, 97
GluI GCNGC 4 cut(s) 99, 235, 1051, 1118
HaeII RGCGCY 1 cut(s) 72
HaeIII GGCC 1 cut(s) 484
HapII CCGG 4 cut(s) 288, 653, 707, 904
HgaI GACGC 1 cut(s) 70
HhaI GCGC 2 cut(s) 71, 98
Hin1I GRCGYC 2 cut(s) 69, 81
Hin1II CATG 1 cut(s) 575
Hin6I GCGC 2 cut(s) 69, 96
HinP1I GCGC 2 cut(s) 69, 96
HincII GTYRAC 2 cut(s) 262, 334
HindII GTYRAC 2 cut(s) 262, 334
HinfI GANTC 7 cut(s) 48, 431, 487, 646, 702, 833, 1130
HpaII CCGG 4 cut(s) 288, 653, 707, 904
HphI GGTGA 2 cut(s) 115, 485
Hpy166II GTNNAC 4 cut(s) 106, 262, 273, 334
Hpy188I TCNGA 6 cut(s) 181, 214, 380, 391, 446, 990
Hpy188III TCNNGA 4 cut(s) 45, 359, 491, 962
Hpy8I GTNNAC 4 cut(s) 106, 262, 273, 334
Hpy99I CGWCG 7 cut(s) 10, 227, 245, 263, 266, 329, 332
HpyAV CCTTC 4 cut(s) 361, 441, 509, 993
HpyCH4III ACNGT 2 cut(s) 241, 338
HpyCH4IV ACGT 4 cut(s) 269, 347, 636, 780
HpyCH4V TGCA 9 cut(s) 106, 562, 687, 723, 766, 775, 784, 950, 1050
HpyF10VI GCNNNNNNNGC 7 cut(s) 98, 254, 772, 781, 883, 892, 1047
HpyF3I CTNAG 2 cut(s) 143, 749
HpySE526I ACGT 4 cut(s) 269, 347, 636, 780
Hsp92I GRCGYC 2 cut(s) 69, 81
Hsp92II CATG 1 cut(s) 575
HspAI GCGC 2 cut(s) 69, 96
KasI GGCGCC 1 cut(s) 68
Ksp22I TGATCA 1 cut(s) 475
Kzo9I GATC 7 cut(s) 181, 205, 475, 518, 727, 768, 1104
LmnI GCTCC 1 cut(s) 156
Lsp1109I GCAGC 2 cut(s) 1062, 1104
LweI GCATC 6 cut(s) 148, 535, 784, 898, 937, 1036
MaeI CTAG 1 cut(s) 89
MaeII ACGT 4 cut(s) 269, 347, 636, 780
MaeIII GTNAC 1 cut(s) 888
MalI GATC 7 cut(s) 183, 207, 477, 520, 729, 770, 1106
MbiI CCGCTC 1 cut(s) 189
MboI GATC 7 cut(s) 181, 205, 475, 518, 727, 768, 1104
MboII GAAGA 6 cut(s) 19, 641, 655, 722, 950, 953
MfeI CAATTG 1 cut(s) 827
MflI RGATCY 3 cut(s) 727, 768, 1104
MhlI GDGCHC 2 cut(s) 108, 429
MluCI AATT 6 cut(s) 118, 405, 436, 827, 850, 933
Mly113I GGCGCC 1 cut(s) 69
MlyI GAGTC 2 cut(s) 711, 842
MmeI TCCRAC 1 cut(s) 237
MseI TTAA 2 cut(s) 374, 1135
MslI CAYNNNNRTG 1 cut(s) 1081
MspI CCGG 4 cut(s) 288, 653, 707, 904
MspR9I CCNGG 3 cut(s) 61, 707, 905
MunI CAATTG 1 cut(s) 827
MvaI CCWGG 1 cut(s) 61
MvnI CGCG 1 cut(s) 234
MwoI GCNNNNNNNGC 7 cut(s) 98, 254, 772, 781, 883, 892, 1047
NarI GGCGCC 1 cut(s) 69
NciI CCSGG 2 cut(s) 707, 905
NdeI CATATG 1 cut(s) 983
NdeII GATC 7 cut(s) 181, 205, 475, 518, 727, 768, 1104
NlaIII CATG 1 cut(s) 575
NlaIV GGNNCC 3 cut(s) 70, 238, 453
NspI RCATGY 1 cut(s) 575
OliI CACNNNNGTG 1 cut(s) 1081
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PfeI GAWTC 5 cut(s) 48, 431, 487, 646, 1130
PflFI GACNNNGTC 1 cut(s) 23
PfoI TCCNGGA 1 cut(s) 705
PkrI GCNGC 4 cut(s) 100, 236, 1052, 1119
PleI GAGTC 2 cut(s) 710, 841
PluTI GGCGCC 1 cut(s) 72
PmaCI CACGTG 2 cut(s) 270, 781
PmlI CACGTG 2 cut(s) 270, 781
PpsI GAGTC 2 cut(s) 710, 841
Ppu21I YACGTR 2 cut(s) 270, 781
Psp1406I AACGTT 1 cut(s) 636
Psp6I CCWGG 1 cut(s) 59
PspCI CACGTG 2 cut(s) 270, 781
PspGI CCWGG 1 cut(s) 59
PspN4I GGNNCC 3 cut(s) 70, 238, 453
PstI CTGCAG 1 cut(s) 689
PsuI RGATCY 3 cut(s) 727, 768, 1104
PsyI GACNNNGTC 1 cut(s) 23
RsaI GTAC 4 cut(s) 231, 294, 340, 570
RsaNI GTAC 4 cut(s) 230, 293, 339, 569
RseI CAYNNNNRTG 1 cut(s) 1081
SalI GTCGAC 1 cut(s) 260
SaqAI TTAA 2 cut(s) 374, 1135
SatI GCNGC 4 cut(s) 99, 235, 1051, 1118
Sau3AI GATC 7 cut(s) 181, 205, 475, 518, 727, 768, 1104
ScaI AGTACT 1 cut(s) 340
SchI GAGTC 2 cut(s) 711, 842
ScrFI CCNGG 3 cut(s) 61, 707, 905
SduI GDGCHC 2 cut(s) 108, 429
SfaNI GCATC 6 cut(s) 148, 535, 784, 898, 937, 1036
SfcI CTRYAG 2 cut(s) 675, 685
SfoI GGCGCC 1 cut(s) 70
SgrDI CGTCGACG 1 cut(s) 260
SmiMI CAYNNNNRTG 1 cut(s) 1081
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
Sse9I AATT 6 cut(s) 118, 405, 436, 827, 850, 933
SsiI CCGC 8 cut(s) 99, 165, 187, 234, 255, 1003, 1023, 1108
SspDI GGCGCC 1 cut(s) 68
SspMI CTAG 1 cut(s) 89
StyD4I CCNGG 3 cut(s) 59, 705, 903
StyI CCWWGG 1 cut(s) 837
TaaI ACNGT 2 cut(s) 241, 338
TaiI ACGT 4 cut(s) 272, 350, 639, 783
TaqI TCGA 5 cut(s) 204, 243, 261, 1030, 1062
TasI AATT 6 cut(s) 118, 405, 436, 827, 850, 933
TatI WGTACW 1 cut(s) 338
TauI GCSGC 2 cut(s) 101, 237
TfiI GAWTC 5 cut(s) 48, 431, 487, 646, 1130
Tru1I TTAA 2 cut(s) 374, 1135
Tru9I TTAA 2 cut(s) 374, 1135
TscAI CASTG 1 cut(s) 18
TseI GCWGC 2 cut(s) 1050, 1117
TspDTI ATGAA 1 cut(s) 1003
TspGWI ACGGA 2 cut(s) 100, 143
TspRI CASTG 1 cut(s) 18
Tth111I GACNNNGTC 1 cut(s) 23
VneI GTGCAC 1 cut(s) 104
XceI RCATGY 1 cut(s) 575
XmiI GTMKAC 1 cut(s) 261
XspI CTAG 1 cut(s) 89
ZrmI AGTACT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.