AT1G67060

Peptidase M50B-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001267
Physical Location & Seq
Forward (+)
25036502 .. 25039439
2938 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G67060.2

Sequence Viewer

Length: 705 bp
ATGGATTCGCCAAACTGGGAACTCCGTGGTTGTTGTAACCGGAATCAAAATACTTTCCTCATCACCATCGGAGTCTTCACCGTAGTTATCCTTCTGCTATGGAGGACATTTCTATTGACTCCATTTAAACTCATTACGGTGTTTTTGCACGAAGCTAGTCATGCCGTTGCTTGCAAGCTTACATGTGGAGATGTAGAGGGGATGGAGGTGAATGCAAATGAAGGGGGTTCGACCACAACACGTGGTGGCATTTATTGGTTGATCTTACCTGCTGGCTATCTTGGCTCATCATTTTGGGGAATGGCATTGATTCTTGCATCTACCAATCTGCTTACAGCAAGAATAGCTGCTGCTGGTCTTGGTCTTGCTTTATTCATCGTTCTCTTCATTGCCAAAAACTGGACGCTTCGAGGGCTTTGTATAGGTTTCATAGTTTTCCTCGCTGTCATATGGGTTCTACAAGAGTTAACTACAGTCAAAATTCTCCGTTATGTCATTCTGTTTATTGGTGTGATGAATAGCTTATTCTCAGTTTACGATATCTATGATGATTTGATATCTCGGAGGGTCCATTCGAGCGATGCTGAGAAGTTCGCAGAGATCTGTCCTTGCTGTACCGGTTGTGGCTGGGGTGTCATCTGGGGAATGATATCATTTGCGTTTCTTTGTGCATCGCTCTATCTCGGGCTAGTGATCCTATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M50B

Protein Analysis

234

Amino Acids

25.78

Weight (kDa)

6.79

Isoelectric Point (pI)

31.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M50B PF13398 1 - 157 2e-36 Peptidase M50B-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 277
AccB7I CCANNNNNTGG 1 cut(s) 399
AclWI GGATC 1 cut(s) 688
AcsI RAATTY 1 cut(s) 480
AcvI CACGTG 1 cut(s) 242
AdeI CACNNNGTG 1 cut(s) 245
AfaI GTAC 1 cut(s) 616
AfiI CCNNNNNNNGG 1 cut(s) 399
AflIII ACRYGT 2 cut(s) 182, 239
AgeI ACCGGT 1 cut(s) 617
AluBI AGCT 4 cut(s) 155, 178, 347, 522
AluI AGCT 4 cut(s) 155, 178, 347, 522
AlwI GGATC 1 cut(s) 688
Ama87I CYCGRG 1 cut(s) 683
ApeKI GCWGC 2 cut(s) 347, 350
ApoI RAATTY 1 cut(s) 480
AsiGI ACCGGT 1 cut(s) 617
AspS9I GGNCC 1 cut(s) 568
AsuHPI GGTGA 3 cut(s) 55, 70, 220
AvaI CYCGRG 1 cut(s) 683
AvaII GGWCC 1 cut(s) 568
BarI GAAGNNNNNNTAC 2 cut(s) 75, 107
BbrPI CACGTG 1 cut(s) 242
BbsI GAAGAC 1 cut(s) 67
BbvI GCAGC 2 cut(s) 334, 337
BccI CCATC 2 cut(s) 74, 196
BceAI ACGGC 1 cut(s) 149
BfaI CTAG 2 cut(s) 156, 689
BfmI CTRYAG 1 cut(s) 471
BfuAI ACCTGC 1 cut(s) 277
BglII AGATCT 1 cut(s) 600
BisI GCNGC 2 cut(s) 348, 351
BlsI GCNGC 2 cut(s) 349, 352
Bme18I GGWCC 1 cut(s) 568
BmeT110I CYCGRG 1 cut(s) 683
BmgT120I GGNCC 1 cut(s) 568
BmiI GGNNCC 1 cut(s) 569
BmrI ACTGGG 1 cut(s) 25
BmsI GCATC 3 cut(s) 326, 571, 680
BmuI ACTGGG 1 cut(s) 25
BpiI GAAGAC 1 cut(s) 67
BsaAI YACGTR 1 cut(s) 242
BsaJI CCNNGG 1 cut(s) 25
BsaWI WCCGGW 2 cut(s) 39, 617
Bsc4I CCNNNNNNNGG 1 cut(s) 399
Bse118I RCCGGY 1 cut(s) 617
Bse1I ACTGG 2 cut(s) 20, 404
Bse3DI GCAATG 1 cut(s) 387
BseDI CCNNGG 1 cut(s) 25
BseGI GGATG 1 cut(s) 207
BseLI CCNNNNNNNGG 1 cut(s) 399
BseMI GCAATG 1 cut(s) 387
BseMII CTCAG 2 cut(s) 543, 576
BseNI ACTGG 2 cut(s) 20, 404
BseXI GCAGC 2 cut(s) 334, 337
BseYI CCCAGC 1 cut(s) 627
BshTI ACCGGT 1 cut(s) 617
BsiHKCI CYCGRG 1 cut(s) 683
BsiSI CCGG 2 cut(s) 40, 618
BslI CCNNNNNNNGG 1 cut(s) 399
BsmI GAATGC 1 cut(s) 217
BsoBI CYCGRG 1 cut(s) 683
Bsp143I GATC 3 cut(s) 261, 600, 693
BspCNI CTCAG 2 cut(s) 542, 577
BspLI GGNNCC 1 cut(s) 569
BspMI ACCTGC 1 cut(s) 277
BspPI GGATC 1 cut(s) 688
BsrDI GCAATG 1 cut(s) 387
BsrFI RCCGGY 1 cut(s) 617
BsrI ACTGG 2 cut(s) 20, 404
BssAI RCCGGY 1 cut(s) 617
BssECI CCNNGG 1 cut(s) 25
BssMI GATC 3 cut(s) 261, 600, 693
Bst4CI ACNGT 3 cut(s) 82, 139, 475
Bst6I CTCTTC 1 cut(s) 389
BstBAI YACGTR 1 cut(s) 242
BstC8I GCNNGC 3 cut(s) 172, 176, 274
BstDEI CTNAG 2 cut(s) 529, 585
BstDSI CCRYGG 1 cut(s) 25
BstF5I GGATG 1 cut(s) 207
BstKTI GATC 3 cut(s) 264, 603, 696
BstMBI GATC 3 cut(s) 261, 600, 693
BstMWI GCNNNNNNNGC 4 cut(s) 161, 282, 344, 412
BstNSI RCATGY 1 cut(s) 186
BstSFI CTRYAG 1 cut(s) 471
BstV1I GCAGC 2 cut(s) 334, 337
BstV2I GAAGAC 1 cut(s) 67
BstX2I RGATCY 1 cut(s) 600
BstYI RGATCY 1 cut(s) 600
BtgI CCRYGG 1 cut(s) 25
BtgZI GCGATG 2 cut(s) 594, 657
BtsCI GGATG 1 cut(s) 207
BveI ACCTGC 1 cut(s) 277
Cac8I GCNNGC 3 cut(s) 172, 176, 274
Cfr10I RCCGGY 1 cut(s) 617
Cfr13I GGNCC 1 cut(s) 568
CseI GACGC 1 cut(s) 412
Csp6I GTAC 1 cut(s) 615
CspAI ACCGGT 1 cut(s) 617
CviAII CATG 2 cut(s) 161, 183
CviJI RGCY 9 cut(s) 155, 178, 276, 285, 347, 415, 522, 627, 688
CviKI_1 RGCY 9 cut(s) 155, 178, 276, 285, 347, 415, 522, 627, 688
CviQI GTAC 1 cut(s) 615
DdeI CTNAG 2 cut(s) 529, 585
DpnI GATC 3 cut(s) 263, 602, 695
DpnII GATC 3 cut(s) 261, 600, 693
DraI TTTAAA 1 cut(s) 127
DraIII CACNNNGTG 1 cut(s) 245
Eam1104I CTCTTC 1 cut(s) 389
EarI CTCTTC 1 cut(s) 389
Eco32I GATATC 3 cut(s) 541, 558, 651
Eco47I GGWCC 1 cut(s) 568
Eco72I CACGTG 1 cut(s) 242
Eco88I CYCGRG 1 cut(s) 683
EcoRV GATATC 3 cut(s) 541, 558, 651
FaeI CATG 2 cut(s) 164, 186
FatI CATG 2 cut(s) 160, 182
FauNDI CATATG 1 cut(s) 449
Fnu4HI GCNGC 2 cut(s) 348, 351
FokI GGATG 1 cut(s) 214
Fsp4HI GCNGC 2 cut(s) 348, 351
FspBI CTAG 2 cut(s) 156, 689
GluI GCNGC 2 cut(s) 348, 351
GsaI CCCAGC 1 cut(s) 631
HapII CCGG 2 cut(s) 40, 618
HgaI GACGC 1 cut(s) 412
Hin1II CATG 2 cut(s) 164, 186
HincII GTYRAC 1 cut(s) 468
HindII GTYRAC 1 cut(s) 468
HindIII AAGCTT 1 cut(s) 176
HinfI GANTC 5 cut(s) 5, 43, 72, 118, 310
HpaI GTTAAC 1 cut(s) 468
HpaII CCGG 2 cut(s) 40, 618
HphI GGTGA 3 cut(s) 55, 70, 220
Hpy166II GTNNAC 2 cut(s) 468, 535
Hpy188I TCNGA 2 cut(s) 71, 564
Hpy8I GTNNAC 2 cut(s) 468, 535
HpyAV CCTTC 2 cut(s) 101, 215
HpyCH4III ACNGT 3 cut(s) 82, 139, 475
HpyCH4IV ACGT 1 cut(s) 241
HpyCH4V TGCA 5 cut(s) 148, 174, 215, 317, 671
HpyF10VI GCNNNNNNNGC 4 cut(s) 161, 282, 344, 412
HpyF3I CTNAG 2 cut(s) 529, 585
HpySE526I ACGT 1 cut(s) 241
Hsp92II CATG 2 cut(s) 164, 186
KspAI GTTAAC 1 cut(s) 468
Kzo9I GATC 3 cut(s) 261, 600, 693
LpnPI CCDG 8 cut(s) 53, 258, 282, 339, 385, 613, 625, 631
Lsp1109I GCAGC 2 cut(s) 334, 337
LweI GCATC 3 cut(s) 326, 571, 680
MaeI CTAG 2 cut(s) 156, 689
MaeII ACGT 1 cut(s) 241
MaeIII GTNAC 1 cut(s) 35
MalI GATC 3 cut(s) 263, 602, 695
MboI GATC 3 cut(s) 261, 600, 693
MboII GAAGA 2 cut(s) 67, 376
MflI RGATCY 1 cut(s) 600
MluCI AATT 1 cut(s) 480
MlyI GAGTC 2 cut(s) 81, 112
MnlI CCTC 7 cut(s) 68, 96, 190, 199, 404, 449, 558
MseI TTAA 2 cut(s) 126, 467
MslI CAYNNNNRTG 1 cut(s) 137
MspI CCGG 2 cut(s) 40, 618
Mva1269I GAATGC 1 cut(s) 217
MwoI GCNNNNNNNGC 4 cut(s) 161, 282, 344, 412
NdeI CATATG 1 cut(s) 449
NdeII GATC 3 cut(s) 261, 600, 693
NlaIII CATG 2 cut(s) 164, 186
NlaIV GGNNCC 1 cut(s) 569
NspI RCATGY 1 cut(s) 186
PciI ACATGT 1 cut(s) 182
PctI GAATGC 1 cut(s) 217
PfeI GAWTC 3 cut(s) 5, 43, 310
PflMI CCANNNNNTGG 1 cut(s) 399
PinAI ACCGGT 1 cut(s) 617
PkrI GCNGC 2 cut(s) 349, 352
PleI GAGTC 2 cut(s) 80, 112
PmaCI CACGTG 1 cut(s) 242
PmlI CACGTG 1 cut(s) 242
PpsI GAGTC 2 cut(s) 80, 112
Ppu21I YACGTR 1 cut(s) 242
PscI ACATGT 1 cut(s) 182
PspCI CACGTG 1 cut(s) 242
PspFI CCCAGC 1 cut(s) 627
PspN4I GGNNCC 1 cut(s) 569
PspPI GGNCC 1 cut(s) 568
PsuI RGATCY 1 cut(s) 600
RsaI GTAC 1 cut(s) 616
RsaNI GTAC 1 cut(s) 615
RseI CAYNNNNRTG 1 cut(s) 137
SaqAI TTAA 2 cut(s) 126, 467
SatI GCNGC 2 cut(s) 348, 351
Sau3AI GATC 3 cut(s) 261, 600, 693
Sau96I GGNCC 1 cut(s) 568
SchI GAGTC 2 cut(s) 81, 112
SetI ASST 8 cut(s) 157, 180, 210, 244, 271, 349, 427, 524
SfaNI GCATC 3 cut(s) 326, 571, 680
SfcI CTRYAG 1 cut(s) 471
SinI GGWCC 1 cut(s) 568
SmiMI CAYNNNNRTG 1 cut(s) 137
Sse9I AATT 1 cut(s) 480
SspMI CTAG 2 cut(s) 156, 689
TaaI ACNGT 3 cut(s) 82, 139, 475
TaiI ACGT 1 cut(s) 244
TaqI TCGA 3 cut(s) 230, 409, 575
TasI AATT 1 cut(s) 480
TfiI GAWTC 3 cut(s) 5, 43, 310
Tru1I TTAA 2 cut(s) 126, 467
Tru9I TTAA 2 cut(s) 126, 467
TseI GCWGC 2 cut(s) 347, 350
TspDTI ATGAA 5 cut(s) 234, 364, 376, 418, 530
TspGWI ACGGA 2 cut(s) 14, 476
Van91I CCANNNNNTGG 1 cut(s) 399
VpaK11BI GGWCC 1 cut(s) 568
XapI RAATTY 1 cut(s) 480
XceI RCATGY 1 cut(s) 186
XspI CTAG 2 cut(s) 156, 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.