Rw7G006310

Peptidase M50B-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001267
Physical Location & Seq
Reverse (-)
5349701 .. 5352652
2952 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G006310.1

Sequence Viewer

Length: 702 bp
ATGGAGCCGAATTGGGGACTGAAGAATTGCTGTAACCATGACCAAGTGGTGTTTCTCATTACTGTTTCTGTCTGCACTGTTGTTATACTTGCGCTTTGGAGGACAATACTGCTGAGACCGTTTAAGCTTGTCACTGTCTTTCTTCATGAGGCTAGCCATGCTATCGCTTGTAAACTAACATGTGGTCATGTGGAAGGAATGCAAGTTCATGCAGATGAAGGTGGAGCAACACAAACCCGTGGTGGTGTATACTGGTTAATTCTGCCAGCTGGATATCTAGGTTCATCCTTTTGGGGGATGCTCTTGATTCTTGCATCTACAAATCTTCTTACAGCACGGATAGCCGCTGGATGTTTTCTAGCTGCTCTATTTATTACACTCTTTATAGCTAAAAATTGGACACTTCGAGGACTTTGTATAGGATTCATTCTGTTCCTTGGTGTAATTTGGGCTCTGCAAGAAACAACAAAAGTTCGTGTACTTCGGTATATTATTCTGTTTATAGGTGTGATGAACAGCTTATTTTCAGTATATGATATCTATGATGATCTGATATCCCGTAGAGTTCATTCTAGTGATGCTGAGAAGTTTGCAGTAGAGTGTCCTTGCTGTACTGGTTGTGGATGGGGTGTCATATGGGGCTTAATATCTTTCTTGCTTCTCTGTGGAGCCATGTACCTTGGTCTTGTGATTTTGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M50B

Protein Analysis

233

Amino Acids

25.71

Weight (kDa)

7.98

Isoelectric Point (pI)

30.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M50B PF13398 24 - 223 3.9e-54 Peptidase M50B-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 249
AciI CCGC 1 cut(s) 345
AcuI CTGAAG 1 cut(s) 41
AfaI GTAC 3 cut(s) 480, 613, 677
AfiI CCNNNNNNNGG 2 cut(s) 14, 294
AflIII ACRYGT 1 cut(s) 179
AjuI GAANNNNNNNTTGG 2 cut(s) 36, 68
AloI GAACNNNNNNTCC 2 cut(s) 189, 221
AluBI AGCT 5 cut(s) 127, 269, 362, 389, 519
AluI AGCT 5 cut(s) 127, 269, 362, 389, 519
Alw26I GTCTC 1 cut(s) 109
ApeKI GCWGC 1 cut(s) 362
AspLEI GCGC 1 cut(s) 94
AsuNHI GCTAGC 1 cut(s) 152
BanII GRGCYC 1 cut(s) 454
BbvI GCAGC 1 cut(s) 349
BccI CCATC 1 cut(s) 618
BcoDI GTCTC 1 cut(s) 109
BfaI CTAG 4 cut(s) 153, 278, 359, 573
BisI GCNGC 2 cut(s) 345, 363
BlsI GCNGC 2 cut(s) 346, 364
BmiI GGNNCC 2 cut(s) 6, 670
BmsI GCATC 3 cut(s) 288, 323, 568
BmtI GCTAGC 1 cut(s) 156
BsaI GGTCTC 1 cut(s) 109
BsaJI CCNNGG 3 cut(s) 238, 436, 679
Bsc4I CCNNNNNNNGG 2 cut(s) 14, 294
Bse1I ACTGG 2 cut(s) 257, 619
BseDI CCNNGG 3 cut(s) 238, 436, 679
BseGI GGATG 4 cut(s) 284, 303, 356, 629
BseLI CCNNNNNNNGG 2 cut(s) 14, 294
BseMII CTCAG 2 cut(s) 104, 573
BseNI ACTGG 2 cut(s) 257, 619
BseXI GCAGC 1 cut(s) 349
BsgI GTGCAG 1 cut(s) 58
BslFI GGGAC 1 cut(s) 30
BslI CCNNNNNNNGG 2 cut(s) 14, 294
BsmAI GTCTC 1 cut(s) 109
BsmFI GGGAC 1 cut(s) 30
BsmI GAATGC 1 cut(s) 204
Bso31I GGTCTC 1 cut(s) 109
Bsp1286I GDGCHC 1 cut(s) 454
Bsp143I GATC 1 cut(s) 547
BspACI CCGC 1 cut(s) 345
BspCNI CTCAG 2 cut(s) 105, 574
BspHI TCATGA 1 cut(s) 145
BspLI GGNNCC 2 cut(s) 6, 670
BspOI GCTAGC 1 cut(s) 156
BspTNI GGTCTC 1 cut(s) 109
BsrI ACTGG 2 cut(s) 257, 619
BssECI CCNNGG 3 cut(s) 238, 436, 679
BssMI GATC 1 cut(s) 547
BssNAI GTATAC 1 cut(s) 250
BssT1I CCWWGG 2 cut(s) 436, 679
Bst1107I GTATAC 1 cut(s) 250
Bst4CI ACNGT 4 cut(s) 64, 79, 120, 136
BstC8I GCNNGC 2 cut(s) 154, 267
BstDEI CTNAG 2 cut(s) 113, 582
BstDSI CCRYGG 1 cut(s) 238
BstF5I GGATG 4 cut(s) 284, 303, 356, 629
BstHHI GCGC 1 cut(s) 94
BstKTI GATC 1 cut(s) 550
BstMAI GTCTC 1 cut(s) 109
BstMBI GATC 1 cut(s) 547
BstMWI GCNNNNNNNGC 2 cut(s) 158, 341
BstNSI RCATGY 1 cut(s) 183
BstV1I GCAGC 1 cut(s) 349
BstZ17I GTATAC 1 cut(s) 250
BtgI CCRYGG 1 cut(s) 238
BtsCI GGATG 4 cut(s) 284, 303, 356, 629
BtsIMutI CAGTG 2 cut(s) 75, 132
Cac8I GCNNGC 2 cut(s) 154, 267
CciI TCATGA 1 cut(s) 145
CfoI GCGC 1 cut(s) 94
Csp6I GTAC 3 cut(s) 479, 612, 676
CviAII CATG 7 cut(s) 38, 146, 158, 180, 188, 209, 673
CviQI GTAC 3 cut(s) 479, 612, 676
DdeI CTNAG 2 cut(s) 113, 582
DpnI GATC 1 cut(s) 549
DpnII GATC 1 cut(s) 547
Eco130I CCWWGG 2 cut(s) 436, 679
Eco24I GRGCYC 1 cut(s) 454
Eco31I GGTCTC 1 cut(s) 109
Eco32I GATATC 3 cut(s) 275, 538, 555
Eco57I CTGAAG 1 cut(s) 41
EcoRV GATATC 3 cut(s) 275, 538, 555
EcoT14I CCWWGG 2 cut(s) 436, 679
EcoT38I GRGCYC 1 cut(s) 454
ErhI CCWWGG 2 cut(s) 436, 679
FaeI CATG 7 cut(s) 41, 149, 161, 183, 191, 212, 676
FaqI GGGAC 1 cut(s) 30
FatI CATG 7 cut(s) 37, 145, 157, 179, 187, 208, 672
FauNDI CATATG 1 cut(s) 635
FblI GTMKAC 1 cut(s) 249
Fnu4HI GCNGC 2 cut(s) 345, 363
FokI GGATG 4 cut(s) 271, 310, 363, 636
FriOI GRGCYC 1 cut(s) 454
Fsp4HI GCNGC 2 cut(s) 345, 363
FspBI CTAG 4 cut(s) 153, 278, 359, 573
GlaI GCGC 1 cut(s) 93
GluI GCNGC 2 cut(s) 345, 363
HhaI GCGC 1 cut(s) 94
Hin1II CATG 7 cut(s) 41, 149, 161, 183, 191, 212, 676
Hin6I GCGC 1 cut(s) 92
HinP1I GCGC 1 cut(s) 92
HindIII AAGCTT 1 cut(s) 125
HinfI GANTC 2 cut(s) 307, 423
Hpy166II GTNNAC 3 cut(s) 173, 250, 479
Hpy188I TCNGA 1 cut(s) 552
Hpy188III TCNNGA 2 cut(s) 146, 304
Hpy8I GTNNAC 3 cut(s) 173, 250, 479
HpyAV CCTTC 2 cut(s) 188, 212
HpyCH4III ACNGT 4 cut(s) 64, 79, 120, 136
HpyCH4V TGCA 6 cut(s) 75, 202, 212, 314, 457, 593
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 341
HpyF3I CTNAG 2 cut(s) 113, 582
Hsp92II CATG 7 cut(s) 41, 149, 161, 183, 191, 212, 676
HspAI GCGC 1 cut(s) 92
Kzo9I GATC 1 cut(s) 547
LmnI GCTCC 3 cut(s) 4, 224, 668
LpnPI CCDG 5 cut(s) 238, 255, 279, 333, 600
Lsp1109I GCAGC 1 cut(s) 349
LweI GCATC 3 cut(s) 288, 323, 568
MaeI CTAG 4 cut(s) 153, 278, 359, 573
MaeIII GTNAC 2 cut(s) 32, 130
MalI GATC 1 cut(s) 549
MboI GATC 1 cut(s) 547
MboII GAAGA 3 cut(s) 34, 134, 317
MhlI GDGCHC 1 cut(s) 454
MluCI AATT 5 cut(s) 10, 25, 258, 394, 444
MnlI CCTC 3 cut(s) 93, 142, 401
MseI TTAA 3 cut(s) 123, 257, 644
MslI CAYNNNNRTG 2 cut(s) 213, 573
MspA1I CMGCKG 2 cut(s) 269, 347
Mva1269I GAATGC 1 cut(s) 204
MwoI GCNNNNNNNGC 2 cut(s) 158, 341
NdeI CATATG 1 cut(s) 635
NdeII GATC 1 cut(s) 547
NheI GCTAGC 1 cut(s) 152
NlaIII CATG 7 cut(s) 41, 149, 161, 183, 191, 212, 676
NlaIV GGNNCC 2 cut(s) 6, 670
NmuCI GTSAC 1 cut(s) 130
NspI RCATGY 1 cut(s) 183
PagI TCATGA 1 cut(s) 145
PciI ACATGT 1 cut(s) 179
PctI GAATGC 1 cut(s) 204
PfeI GAWTC 2 cut(s) 307, 423
PkrI GCNGC 2 cut(s) 346, 364
PscI ACATGT 1 cut(s) 179
PspN4I GGNNCC 2 cut(s) 6, 670
PvuII CAGCTG 1 cut(s) 269
RsaI GTAC 3 cut(s) 480, 613, 677
RsaNI GTAC 3 cut(s) 479, 612, 676
RseI CAYNNNNRTG 2 cut(s) 213, 573
SaqAI TTAA 3 cut(s) 123, 257, 644
SatI GCNGC 2 cut(s) 345, 363
Sau3AI GATC 1 cut(s) 547
SduI GDGCHC 1 cut(s) 454
SetI ASST 9 cut(s) 129, 223, 271, 283, 364, 391, 508, 521, 681
SfaNI GCATC 3 cut(s) 288, 323, 568
SmiMI CAYNNNNRTG 2 cut(s) 213, 573
Sse9I AATT 5 cut(s) 10, 25, 258, 394, 444
SsiI CCGC 1 cut(s) 345
SspMI CTAG 4 cut(s) 153, 278, 359, 573
StyI CCWWGG 2 cut(s) 436, 679
TaaI ACNGT 4 cut(s) 64, 79, 120, 136
TaqI TCGA 1 cut(s) 406
TasI AATT 5 cut(s) 10, 25, 258, 394, 444
TatI WGTACW 2 cut(s) 478, 611
TauI GCSGC 1 cut(s) 347
TfiI GAWTC 2 cut(s) 307, 423
Tru1I TTAA 3 cut(s) 123, 257, 644
Tru9I TTAA 3 cut(s) 123, 257, 644
TscAI CASTG 2 cut(s) 82, 139
TseFI GTSAC 1 cut(s) 130
TseI GCWGC 1 cut(s) 362
Tsp45I GTSAC 1 cut(s) 130
TspDTI ATGAA 7 cut(s) 134, 197, 231, 273, 415, 527, 557
TspGWI ACGGA 1 cut(s) 352
TspRI CASTG 2 cut(s) 82, 139
XceI RCATGY 1 cut(s) 183
XmiI GTMKAC 1 cut(s) 249
XspI CTAG 4 cut(s) 153, 278, 359, 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.