Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0001267

Physical Location & Seq

Genomic Coordinates Reverse (-)

32427151 .. 32432840

Gene Structure 5690 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1253200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 783 bp

ATGCCGAATTGGGAGCTGAAGAGCTGTTGCAAGCATGACCAGGTCGTCTTCTTGGCCACCATTGGAGTCTTCACCGTTGTCATCCTCGCGCTTTGGAGGACAATACTACTCACACCTTTTAAGCTCATCACGGTCTTCTTGCATGAAGCAAGCCATGCAGTTGCTTGCATACTCACATGTGGCAAGGTGGAGGGCATCCAGGTTCACGCAAATGAGGGTGGGGTGACACAAACACGTGGTGGTATATATTGGTTGATCTTGCCTGCAGGATATCTGGGTTCATCATTTTGGGGAATGGCTTTAATACTTGCATCGACAAATCTCGTTACTGCTCGAATTGCTGCTGGTTGTCTTGGCGTTGCCTTGCTAGTTGTGCTTTTTGTGGCAAAAAATTGGACACTTCGAGGACTTTGTATTGGATTTATTGTATTCCTTGCTATAGTATGGGTTCTCCAAGAATTAACTAAAGTCCGTATTCTTCGCTATGTCATTCTCTTTATTGGTGTGATGAACAGTTTGTTCTCAGTTTATGATATATATGATGATTTAATATCTAGAAGGGTCAACTCCAGTGATGCTGAAAAATTTGCAGAAATTTGCCCCTGCCCCTGTACTGGGGTTGGATGGGGAGTGATATGGGGATTGATATCGTTTATATTTCTTTCTGCATCGGTGTATCTAGGGCTTGTCATTCTATCGTCCAGGAGATTTTGGATCAGTCGTCCCATATCCGCCCGGAGAGTCAGCTCTCACTTATTTTTTCCACTCTCCCTTGATGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M50B

Protein Analysis

261

Amino Acids

28.79

Weight (kDa)

8.91

Isoelectric Point (pI)

36.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M50B	PF13398	24 - 222	6.3e-57	Peptidase M50B-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001267)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67060 AT1G67060 AT1G67060
fragaria_vesca	FvH4_3g34180 FvH4_5g11892 FvH4_5g11892 FvH4_5g11892 FvH4_5g11892 FvH4_5g11892 FvH4_5g11892 FvH4_5g11892 FvH4_5g11892 FvH4_7g13690 FvH4_7g13690 FvH4_7g13690 FvH4_7g13690 FvH4_7g13690
malus_domestica	MD06G1217400.v1.1 MD06G1217500.v1.1 MD09G1253200.v1.1 MD14G1227700.v1.1
prunus_persica	Prupe.3G143000_v2.0.a1 Prupe.5G222600_v2.0.a1 Prupe.5G222600_v2.0.a1 Prupe.5G222600_v2.0.a1 Prupe.5G222600_v2.0.a1 Prupe.5G222700_v2.0.a1 Prupe.5G222700_v2.0.a1 Prupe.5G222700_v2.0.a1
pyrus_communis	pycom06g19370 pycom06g19380
rosa_chinensis	RchiOBHm_Chr2g0126081 RchiOBHm_Chr7g0185671 RchiOBHm_Chr7g0185681
rosa_multiflora	Rmu_sc0001506.1_g000004 Rmu_sc0008747.1_g000002 Rmu_sc0010190.1_g000003
rosa_roxburghii	Rroxscaffold_3G00268740
rosa_rugosa	Rorug02G0260800 Rorug02G0260900 Rorug02G0261000 Rorug06G0470300 Rorug06G0470400
rosa_samantha	Rh2BG326600 Rh2DG344700 Rh7AG072000 Rh7AG072100 Rh7BG075800 Rh7BG075900 Rh7CG075900 Rh7DG075800 Rh7DG075900
rosa_wichuraiana	Rw2G025770 Rw7G006310 Rw7G006320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	44
AccII	CGCG	1 cut(s)	89
AciI	CCGC	1 cut(s)	732
AclWI	GGATC	1 cut(s)	722
AcoI	YGGCCR	1 cut(s)	54
AcsI	RAATTY	2 cut(s)	584, 594
AcuI	CTGAAG	1 cut(s)	38
AcvI	CACGTG	1 cut(s)	236
AdeI	CACNNNGTG	1 cut(s)	239
AfaI	GTAC	1 cut(s)	613
AfiI	CCNNNNNNNGG	2 cut(s)	614, 615
AflIII	ACRYGT	2 cut(s)	176, 233
AjnI	CCWGG	3 cut(s)	39, 198, 701
AluBI	AGCT	4 cut(s)	16, 24, 124, 747
AluI	AGCT	4 cut(s)	16, 24, 124, 747
AlwI	GGATC	1 cut(s)	722
AoxI	GGCC	1 cut(s)	54
ApeKI	GCWGC	1 cut(s)	341
ApoI	RAATTY	2 cut(s)	584, 594
AspLEI	GCGC	1 cut(s)	91
AsuC2I	CCSGG	1 cut(s)	736
AsuHPI	GGTGA	2 cut(s)	64, 235
BalI	TGGCCA	1 cut(s)	56
BbrPI	CACGTG	1 cut(s)	236
BbsI	GAAGAC	3 cut(s)	40, 61, 127
BbvI	GCAGC	1 cut(s)	328
BccI	CCATC	1 cut(s)	618
BciT130I	CCWGG	3 cut(s)	41, 200, 703
BcnI	CCSGG	1 cut(s)	736
BfaI	CTAG	3 cut(s)	368, 555, 680
BfmI	CTRYAG	2 cut(s)	264, 438
BisI	GCNGC	1 cut(s)	342
BlsI	GCNGC	1 cut(s)	343
Bme1390I	CCNGG	4 cut(s)	41, 200, 703, 736
BmrFI	CCNGG	4 cut(s)	41, 200, 703, 736
BmrI	ACTGGG	1 cut(s)	624
BmsI	GCATC	4 cut(s)	204, 320, 565, 677
BmuI	ACTGGG	1 cut(s)	624
BpiI	GAAGAC	3 cut(s)	40, 61, 127
BpmI	CTGGAG	1 cut(s)	553
BpuMI	CCSGG	1 cut(s)	736
BsaAI	YACGTR	1 cut(s)	236
BsaBI	GATNNNNATC	1 cut(s)	646
Bsc4I	CCNNNNNNNGG	2 cut(s)	614, 615
Bse1I	ACTGG	2 cut(s)	570, 619
Bse8I	GATNNNNATC	1 cut(s)	646
BseBI	CCWGG	3 cut(s)	41, 200, 703
BseGI	GGATG	3 cut(s)	81, 195, 629
BseJI	GATNNNNATC	1 cut(s)	646
BseLI	CCNNNNNNNGG	2 cut(s)	614, 615
BseMII	CTCAG	1 cut(s)	537
BseNI	ACTGG	2 cut(s)	570, 619
BseXI	GCAGC	1 cut(s)	328
Bsh1236I	CGCG	1 cut(s)	89
BshFI	GGCC	1 cut(s)	56
BsiSI	CCGG	1 cut(s)	736
BslFI	GGGAC	1 cut(s)	708
BslI	CCNNNNNNNGG	2 cut(s)	614, 615
BsmFI	GGGAC	1 cut(s)	708
BsnI	GGCC	1 cut(s)	56
Bsp143I	GATC	2 cut(s)	255, 714
BspACI	CCGC	1 cut(s)	732
BspANI	GGCC	1 cut(s)	56
BspCNI	CTCAG	1 cut(s)	536
BspFNI	CGCG	1 cut(s)	89
BspMAI	CTGCAG	1 cut(s)	268
BspPI	GGATC	1 cut(s)	722
BspQI	GCTCTTC	1 cut(s)	14
BsrI	ACTGG	2 cut(s)	570, 619
BssMI	GATC	2 cut(s)	255, 714
Bst2UI	CCWGG	3 cut(s)	41, 200, 703
Bst4CI	ACNGT	3 cut(s)	76, 133, 515
Bst6I	CTCTTC	1 cut(s)	14
BstAPI	GCANNNNNTGC	1 cut(s)	155
BstBAI	YACGTR	1 cut(s)	236
BstC8I	GCNNGC	4 cut(s)	32, 151, 166, 264
BstDEI	CTNAG	1 cut(s)	523
BstF5I	GGATG	3 cut(s)	81, 195, 629
BstFNI	CGCG	1 cut(s)	89
BstHHI	GCGC	1 cut(s)	91
BstKTI	GATC	2 cut(s)	258, 717
BstMBI	GATC	2 cut(s)	255, 714
BstMWI	GCNNNNNNNGC	3 cut(s)	155, 338, 373
BstNI	CCWGG	3 cut(s)	41, 200, 703
BstNSI	RCATGY	1 cut(s)	180
BstSCI	CCNGG	4 cut(s)	39, 198, 701, 734
BstSFI	CTRYAG	2 cut(s)	264, 438
BstUI	CGCG	1 cut(s)	89
BstV1I	GCAGC	1 cut(s)	328
BstV2I	GAAGAC	3 cut(s)	40, 61, 127
BsuRI	GGCC	1 cut(s)	56
BtsCI	GGATG	3 cut(s)	81, 195, 629
BtsIMutI	CAGTG	1 cut(s)	577
Cac8I	GCNNGC	4 cut(s)	32, 151, 166, 264
CfoI	GCGC	1 cut(s)	91
CsiI	ACCWGGT	1 cut(s)	39
Csp6I	GTAC	1 cut(s)	612
CviAII	CATG	4 cut(s)	35, 143, 155, 177
CviJI	RGCY	8 cut(s)	16, 24, 56, 124, 153, 299, 685, 747
CviKI_1	RGCY	8 cut(s)	16, 24, 56, 124, 153, 299, 685, 747
CviQI	GTAC	1 cut(s)	612
DdeI	CTNAG	1 cut(s)	523
DpnI	GATC	2 cut(s)	257, 716
DpnII	GATC	2 cut(s)	255, 714
DraIII	CACNNNGTG	1 cut(s)	239
DrdI	GACNNNNNNGTC	1 cut(s)	44
DseDI	GACNNNNNNGTC	1 cut(s)	44
EaeI	YGGCCR	1 cut(s)	54
Eam1104I	CTCTTC	1 cut(s)	14
EarI	CTCTTC	1 cut(s)	14
EciI	GGCGGA	1 cut(s)	721
Eco32I	GATATC	2 cut(s)	272, 648
Eco57I	CTGAAG	1 cut(s)	38
Eco72I	CACGTG	1 cut(s)	236
EcoRII	CCWGG	3 cut(s)	39, 198, 701
EcoRV	GATATC	2 cut(s)	272, 648
FaeI	CATG	4 cut(s)	38, 146, 158, 180
FaqI	GGGAC	1 cut(s)	708
FatI	CATG	4 cut(s)	34, 142, 154, 176
Fnu4HI	GCNGC	1 cut(s)	342
FokI	GGATG	3 cut(s)	68, 182, 636
Fsp4HI	GCNGC	1 cut(s)	342
FspBI	CTAG	3 cut(s)	368, 555, 680
GlaI	GCGC	1 cut(s)	90
GluI	GCNGC	1 cut(s)	342
GsuI	CTGGAG	1 cut(s)	553
HaeIII	GGCC	1 cut(s)	56
HapII	CCGG	1 cut(s)	736
HhaI	GCGC	1 cut(s)	91
Hin1II	CATG	4 cut(s)	38, 146, 158, 180
Hin6I	GCGC	1 cut(s)	89
HinP1I	GCGC	1 cut(s)	89
HincII	GTYRAC	1 cut(s)	565
HindII	GTYRAC	1 cut(s)	565
HinfI	GANTC	2 cut(s)	66, 741
HpaII	CCGG	1 cut(s)	736
HphI	GGTGA	2 cut(s)	64, 235
Hpy166II	GTNNAC	2 cut(s)	205, 565
Hpy188III	TCNNGA	1 cut(s)	555
Hpy8I	GTNNAC	2 cut(s)	205, 565
HpyAV	CCTTC	1 cut(s)	552
HpyCH4III	ACNGT	3 cut(s)	76, 133, 515
HpyCH4IV	ACGT	1 cut(s)	235
HpyCH4V	TGCA	8 cut(s)	30, 142, 158, 168, 266, 311, 590, 668
HpyF10VI	GCNNNNNNNGC	3 cut(s)	155, 338, 373
HpyF3I	CTNAG	1 cut(s)	523
HpySE526I	ACGT	1 cut(s)	235
Hsp92II	CATG	4 cut(s)	38, 146, 158, 180
HspAI	GCGC	1 cut(s)	89
Kzo9I	GATC	2 cut(s)	255, 714
LguI	GCTCTTC	1 cut(s)	14
LmnI	GCTCC	1 cut(s)	13
Lsp1109I	GCAGC	1 cut(s)	328
LweI	GCATC	4 cut(s)	204, 320, 565, 677
MabI	ACCWGGT	1 cut(s)	39
MaeI	CTAG	3 cut(s)	368, 555, 680
MaeII	ACGT	1 cut(s)	235
MaeIII	GTNAC	2 cut(s)	223, 325
MalI	GATC	2 cut(s)	257, 716
MboI	GATC	2 cut(s)	255, 714
MboII	GAAGA	5 cut(s)	31, 40, 61, 127, 470
MlsI	TGGCCA	1 cut(s)	56
MluCI	AATT	6 cut(s)	7, 336, 391, 458, 584, 594
MluNI	TGGCCA	1 cut(s)	56
MlyI	GAGTC	2 cut(s)	75, 750
MmeI	TCCRAC	1 cut(s)	601
MnlI	CCTC	5 cut(s)	90, 95, 184, 208, 398
Mox20I	TGGCCA	1 cut(s)	56
MscI	TGGCCA	1 cut(s)	56
MseI	TTAA	4 cut(s)	120, 302, 461, 548
MslI	CAYNNNNRTG	1 cut(s)	210
Msp20I	TGGCCA	1 cut(s)	56
MspI	CCGG	1 cut(s)	736
MspR9I	CCNGG	4 cut(s)	41, 200, 703, 736
MvaI	CCWGG	3 cut(s)	41, 200, 703
MvnI	CGCG	1 cut(s)	89
MwoI	GCNNNNNNNGC	3 cut(s)	155, 338, 373
NciI	CCSGG	1 cut(s)	736
NdeII	GATC	2 cut(s)	255, 714
NlaIII	CATG	4 cut(s)	38, 146, 158, 180
NmuCI	GTSAC	1 cut(s)	223
NspI	RCATGY	1 cut(s)	180
PciI	ACATGT	1 cut(s)	176
PciSI	GCTCTTC	1 cut(s)	14
PflFI	GACNNNGTC	1 cut(s)	41
PfoI	TCCNGGA	1 cut(s)	701
PkrI	GCNGC	1 cut(s)	343
PleI	GAGTC	2 cut(s)	74, 749
PmaCI	CACGTG	1 cut(s)	236
PmlI	CACGTG	1 cut(s)	236
PpsI	GAGTC	2 cut(s)	74, 749
Ppu21I	YACGTR	1 cut(s)	236
PscI	ACATGT	1 cut(s)	176
Psp6I	CCWGG	3 cut(s)	39, 198, 701
PspCI	CACGTG	1 cut(s)	236
PspGI	CCWGG	3 cut(s)	39, 198, 701
PstI	CTGCAG	1 cut(s)	268
PsyI	GACNNNGTC	1 cut(s)	41
RsaI	GTAC	1 cut(s)	613
RsaNI	GTAC	1 cut(s)	612
RseI	CAYNNNNRTG	1 cut(s)	210
SapI	GCTCTTC	1 cut(s)	14
SaqAI	TTAA	4 cut(s)	120, 302, 461, 548
SatI	GCNGC	1 cut(s)	342
Sau3AI	GATC	2 cut(s)	255, 714
SbfI	CCTGCAGG	1 cut(s)	268
SchI	GAGTC	2 cut(s)	75, 750
ScrFI	CCNGG	4 cut(s)	41, 200, 703, 736
SdaI	CCTGCAGG	1 cut(s)	268
SetI	ASST	9 cut(s)	18, 26, 45, 118, 126, 189, 204, 238, 749
SexAI	ACCWGGT	1 cut(s)	39
SfaNI	GCATC	4 cut(s)	204, 320, 565, 677
SfcI	CTRYAG	2 cut(s)	264, 438
SmiMI	CAYNNNNRTG	1 cut(s)	210
Sse8387I	CCTGCAGG	1 cut(s)	268
Sse9I	AATT	6 cut(s)	7, 336, 391, 458, 584, 594
SsiI	CCGC	1 cut(s)	732
SspMI	CTAG	3 cut(s)	368, 555, 680
StyD4I	CCNGG	4 cut(s)	39, 198, 701, 734
TaaI	ACNGT	3 cut(s)	76, 133, 515
TaiI	ACGT	1 cut(s)	238
TaqI	TCGA	3 cut(s)	314, 334, 403
TasI	AATT	6 cut(s)	7, 336, 391, 458, 584, 594
TatI	WGTACW	1 cut(s)	611
Tru1I	TTAA	4 cut(s)	120, 302, 461, 548
Tru9I	TTAA	4 cut(s)	120, 302, 461, 548
TscAI	CASTG	1 cut(s)	577
TseFI	GTSAC	1 cut(s)	223
TseI	GCWGC	1 cut(s)	341
Tsp45I	GTSAC	1 cut(s)	223
TspDTI	ATGAA	3 cut(s)	159, 270, 524
TspGWI	ACGGA	1 cut(s)	461
TspRI	CASTG	1 cut(s)	577
Tth111I	GACNNNGTC	1 cut(s)	41
XapI	RAATTY	2 cut(s)	584, 594
XbaI	TCTAGA	1 cut(s)	554
XceI	RCATGY	1 cut(s)	180
XspI	CTAG	3 cut(s)	368, 555, 680

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.