Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0001267

Physical Location & Seq

Genomic Coordinates Reverse (-)

30876999 .. 30878975

Gene Structure 1977 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1227700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 732 bp

ATGGAATTGAATTTGTTGAAATGGAAGCCAAATTGGCAACTCAGAAAATGCTGCAACCACGACCAAGTGATCTTCCTCATCTCCGTTGCTGTTTGCACTGTCGTTATCCTTGTGCTGTGGAGGACGGTACTGATGACACCATTTAAGCTTATAACCGTGTTTCTTCACGAGGTGAGCCATGCAATCGCTTGTCTGCTTACTTGTGGTAAGGTGGAAGGGATTAAAGTTAACGCAGATGAAGGTGGAGTCACACAAACCCGCGGTGGCATATCCTGGCTAATTTTGCCTGCCGGATATCTTGGTTCATCGTTCTGGGGGATGGTATTGATTCTTGCGTCCACAAAACATCTCACTACACAAATCGCTGCTGGCTGTTTCGTTGTTGCTCTAATTATTGTGTTCTTTTTAGCTAAAAATTGGACACTTCGAGGACTTTGTATAGGATTCATTGTTTTATTTGCTGGAATTTGGTATCTGCAAGAAGCAACGCCGGTTCATATGCTTAGGGAAGTGATTCTGTTCACCGGTGTAATGAACAGCTTGTTTTCGGTTTATGATATCTACGATGATCTTATATCGCGTAGAATCAATACGAGTGATGCTGAAAGATTTGCAGATGAGTGTCCCTGCTGTACTGGATGTGGATGGGGTGTCATTTGGGCATTCATATCTTTCACATTTCTCTGCGGATCCGTGTACCTAGCTCTTGTGATTTTATCTCCGCGTGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M50B

Protein Analysis

244

Amino Acids

27.09

Weight (kDa)

7.97

Isoelectric Point (pI)

27.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M50B	PF13398	30 - 230	5.6e-56	Peptidase M50B-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0001267)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67060 AT1G67060 AT1G67060
fragaria_vesca	FvH4_3g34180 FvH4_5g11892 FvH4_5g11892 FvH4_5g11892 FvH4_5g11892 FvH4_5g11892 FvH4_5g11892 FvH4_5g11892 FvH4_5g11892 FvH4_7g13690 FvH4_7g13690 FvH4_7g13690 FvH4_7g13690 FvH4_7g13690
malus_domestica	MD06G1217400.v1.1 MD06G1217500.v1.1 MD09G1253200.v1.1 MD14G1227700.v1.1
prunus_persica	Prupe.3G143000_v2.0.a1 Prupe.5G222600_v2.0.a1 Prupe.5G222600_v2.0.a1 Prupe.5G222600_v2.0.a1 Prupe.5G222600_v2.0.a1 Prupe.5G222700_v2.0.a1 Prupe.5G222700_v2.0.a1 Prupe.5G222700_v2.0.a1
pyrus_communis	pycom06g19370 pycom06g19380
rosa_chinensis	RchiOBHm_Chr2g0126081 RchiOBHm_Chr7g0185671 RchiOBHm_Chr7g0185681
rosa_multiflora	Rmu_sc0001506.1_g000004 Rmu_sc0008747.1_g000002 Rmu_sc0010190.1_g000003
rosa_roxburghii	Rroxscaffold_3G00268740
rosa_rugosa	Rorug02G0260800 Rorug02G0260900 Rorug02G0261000 Rorug06G0470300 Rorug06G0470400
rosa_samantha	Rh2BG326600 Rh2DG344700 Rh7AG072000 Rh7AG072100 Rh7BG075800 Rh7BG075900 Rh7CG075900 Rh7DG075800 Rh7DG075900
rosa_wichuraiana	Rw2G025770 Rw7G006310 Rw7G006320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	152
AccII	CGCG	3 cut(s)	261, 580, 724
AciI	CCGC	4 cut(s)	259, 261, 687, 722
AclWI	GGATC	2 cut(s)	684, 697
AcsI	RAATTY	2 cut(s)	10, 465
AdeI	CACNNNGTG	1 cut(s)	172
AfaI	GTAC	3 cut(s)	129, 634, 698
AgeI	ACCGGT	1 cut(s)	524
AgsI	TTSAA	2 cut(s)	10, 19
AjnI	CCWGG	1 cut(s)	272
AluBI	AGCT	4 cut(s)	148, 410, 540, 704
AluI	AGCT	4 cut(s)	148, 410, 540, 704
AlwI	GGATC	2 cut(s)	684, 697
ApeKI	GCWGC	2 cut(s)	51, 365
ApoI	RAATTY	2 cut(s)	10, 465
AsiGI	ACCGGT	1 cut(s)	524
Asp700I	GAANNNNTTC	1 cut(s)	513
AsuHPI	GGTGA	2 cut(s)	184, 514
BamHI	GGATCC	1 cut(s)	689
BauI	CACGAG	1 cut(s)	167
BbvI	GCAGC	2 cut(s)	38, 352
BccI	CCATC	2 cut(s)	313, 639
BciT130I	CCWGG	1 cut(s)	274
BfaI	CTAG	1 cut(s)	701
BglI	GCCNNNNNGGC	1 cut(s)	34
BisI	GCNGC	2 cut(s)	52, 366
BlsI	GCNGC	2 cut(s)	53, 367
Bme1390I	CCNGG	1 cut(s)	274
BmiI	GGNNCC	1 cut(s)	691
BmrFI	CCNGG	1 cut(s)	274
BmsI	GCATC	1 cut(s)	589
Bpu10I	CCTNAGC	1 cut(s)	503
BsaJI	CCNNGG	1 cut(s)	259
BsaWI	WCCGGW	1 cut(s)	524
Bse118I	RCCGGY	2 cut(s)	490, 524
Bse1I	ACTGG	1 cut(s)	640
BseBI	CCWGG	1 cut(s)	274
BseDI	CCNNGG	1 cut(s)	259
BseGI	GGATG	3 cut(s)	324, 644, 650
BseMII	CTCAG	1 cut(s)	55
BseNI	ACTGG	1 cut(s)	640
BseXI	GCAGC	2 cut(s)	38, 352
Bsh1236I	CGCG	3 cut(s)	261, 580, 724
BshTI	ACCGGT	1 cut(s)	524
BsiSI	CCGG	3 cut(s)	291, 491, 525
BslFI	GGGAC	1 cut(s)	609
BsmFI	GGGAC	1 cut(s)	609
BsmI	GAATGC	1 cut(s)	662
Bsp143I	GATC	3 cut(s)	69, 568, 689
BspACI	CCGC	4 cut(s)	259, 261, 687, 722
BspCNI	CTCAG	1 cut(s)	54
BspFNI	CGCG	3 cut(s)	261, 580, 724
BspLI	GGNNCC	1 cut(s)	691
BspPI	GGATC	2 cut(s)	684, 697
BsrFI	RCCGGY	2 cut(s)	490, 524
BsrI	ACTGG	1 cut(s)	640
BssAI	RCCGGY	2 cut(s)	490, 524
BssECI	CCNNGG	1 cut(s)	259
BssMI	GATC	3 cut(s)	69, 568, 689
BssSI	CACGAG	1 cut(s)	167
Bst2BI	CACGAG	1 cut(s)	167
Bst2UI	CCWGG	1 cut(s)	274
Bst4CI	ACNGT	3 cut(s)	100, 127, 157
BstC8I	GCNNGC	2 cut(s)	288, 370
BstDEI	CTNAG	2 cut(s)	41, 503
BstDSI	CCRYGG	1 cut(s)	259
BstF5I	GGATG	3 cut(s)	324, 644, 650
BstFNI	CGCG	3 cut(s)	261, 580, 724
BstKTI	GATC	3 cut(s)	72, 571, 692
BstMBI	GATC	3 cut(s)	69, 568, 689
BstMWI	GCNNNNNNNGC	2 cut(s)	34, 283
BstNI	CCWGG	1 cut(s)	274
BstSCI	CCNGG	1 cut(s)	272
BstUI	CGCG	3 cut(s)	261, 580, 724
BstV1I	GCAGC	2 cut(s)	38, 352
BstX2I	RGATCY	1 cut(s)	689
BstYI	RGATCY	1 cut(s)	689
BtgI	CCRYGG	1 cut(s)	259
BtsCI	GGATG	3 cut(s)	324, 644, 650
BtsIMutI	CAGTG	1 cut(s)	96
Cac8I	GCNNGC	2 cut(s)	288, 370
Cfr10I	RCCGGY	2 cut(s)	490, 524
Cfr42I	CCGCGG	1 cut(s)	262
CseI	GACGC	1 cut(s)	324
Csp6I	GTAC	3 cut(s)	128, 633, 697
CspAI	ACCGGT	1 cut(s)	524
CviAII	CATG	1 cut(s)	179
CviJI	RGCY	8 cut(s)	28, 148, 177, 277, 372, 410, 540, 704
CviKI_1	RGCY	8 cut(s)	28, 148, 177, 277, 372, 410, 540, 704
CviQI	GTAC	3 cut(s)	128, 633, 697
DdeI	CTNAG	2 cut(s)	41, 503
DpnI	GATC	3 cut(s)	71, 570, 691
DpnII	GATC	3 cut(s)	69, 568, 689
DraIII	CACNNNGTG	1 cut(s)	172
Eco32I	GATATC	2 cut(s)	296, 559
EcoRII	CCWGG	1 cut(s)	272
EcoRV	GATATC	2 cut(s)	296, 559
FaeI	CATG	1 cut(s)	182
FaiI	YATR	9 cut(s)	152, 180, 269, 440, 498, 500, 555, 575, 668
FaqI	GGGAC	1 cut(s)	609
FatI	CATG	1 cut(s)	178
FauI	CCCGC	1 cut(s)	266
FauNDI	CATATG	1 cut(s)	498
Fnu4HI	GCNGC	2 cut(s)	52, 366
FokI	GGATG	3 cut(s)	331, 651, 657
Fsp4HI	GCNGC	2 cut(s)	52, 366
FspBI	CTAG	1 cut(s)	701
GluI	GCNGC	2 cut(s)	52, 366
HapII	CCGG	3 cut(s)	291, 491, 525
HgaI	GACGC	1 cut(s)	324
Hin1II	CATG	1 cut(s)	182
HincII	GTYRAC	1 cut(s)	229
HindII	GTYRAC	1 cut(s)	229
HindIII	AAGCTT	1 cut(s)	146
HinfI	GANTC	5 cut(s)	246, 328, 444, 514, 585
HpaI	GTTAAC	1 cut(s)	229
HpaII	CCGG	3 cut(s)	291, 491, 525
HphI	GGTGA	2 cut(s)	184, 514
Hpy166II	GTNNAC	4 cut(s)	229, 339, 522, 697
Hpy188I	TCNGA	1 cut(s)	44
Hpy188III	TCNNGA	1 cut(s)	167
Hpy8I	GTNNAC	4 cut(s)	229, 339, 522, 697
HpyAV	CCTTC	2 cut(s)	209, 233
HpyCH4III	ACNGT	3 cut(s)	100, 127, 157
HpyCH4V	TGCA	5 cut(s)	54, 96, 182, 478, 614
HpyF10VI	GCNNNNNNNGC	2 cut(s)	34, 283
HpyF3I	CTNAG	2 cut(s)	41, 503
Hsp92II	CATG	1 cut(s)	182
KspAI	GTTAAC	1 cut(s)	229
KspI	CCGCGG	1 cut(s)	262
Kzo9I	GATC	3 cut(s)	69, 568, 689
Lsp1109I	GCAGC	2 cut(s)	38, 352
LweI	GCATC	1 cut(s)	589
MaeI	CTAG	1 cut(s)	701
MaeIII	GTNAC	1 cut(s)	247
MalI	GATC	3 cut(s)	71, 570, 691
MboI	GATC	3 cut(s)	69, 568, 689
MboII	GAAGA	2 cut(s)	64, 155
MflI	RGATCY	1 cut(s)	689
MluCI	AATT	7 cut(s)	5, 10, 31, 279, 390, 415, 465
MlyI	GAGTC	1 cut(s)	255
MnlI	CCTC	4 cut(s)	86, 114, 163, 422
MroXI	GAANNNNTTC	1 cut(s)	513
MseI	TTAA	3 cut(s)	144, 222, 228
MspA1I	CMGCKG	1 cut(s)	261
MspI	CCGG	3 cut(s)	291, 491, 525
MspR9I	CCNGG	1 cut(s)	274
Mva1269I	GAATGC	1 cut(s)	662
MvaI	CCWGG	1 cut(s)	274
MvnI	CGCG	3 cut(s)	261, 580, 724
MwoI	GCNNNNNNNGC	2 cut(s)	34, 283
NdeI	CATATG	1 cut(s)	498
NdeII	GATC	3 cut(s)	69, 568, 689
NlaIII	CATG	1 cut(s)	182
NlaIV	GGNNCC	1 cut(s)	691
NmuCI	GTSAC	1 cut(s)	247
PctI	GAATGC	1 cut(s)	662
PdmI	GAANNNNTTC	1 cut(s)	513
PfeI	GAWTC	4 cut(s)	328, 444, 514, 585
PinAI	ACCGGT	1 cut(s)	524
PkrI	GCNGC	2 cut(s)	53, 367
PleI	GAGTC	1 cut(s)	254
PpsI	GAGTC	1 cut(s)	254
PsiI	TTATAA	1 cut(s)	152
Psp6I	CCWGG	1 cut(s)	272
PspGI	CCWGG	1 cut(s)	272
PspN4I	GGNNCC	1 cut(s)	691
PsuI	RGATCY	1 cut(s)	689
RsaI	GTAC	3 cut(s)	129, 634, 698
RsaNI	GTAC	3 cut(s)	128, 633, 697
SacII	CCGCGG	1 cut(s)	262
SaqAI	TTAA	3 cut(s)	144, 222, 228
SatI	GCNGC	2 cut(s)	52, 366
Sau3AI	GATC	3 cut(s)	69, 568, 689
SchI	GAGTC	1 cut(s)	255
ScrFI	CCNGG	1 cut(s)	274
SetI	ASST	8 cut(s)	150, 174, 213, 244, 412, 542, 702, 706
SfaNI	GCATC	1 cut(s)	589
Sfr303I	CCGCGG	1 cut(s)	262
SgrAI	CRCCGGYG	1 cut(s)	524
SgrBI	CCGCGG	1 cut(s)	262
Sse9I	AATT	7 cut(s)	5, 10, 31, 279, 390, 415, 465
SsiI	CCGC	4 cut(s)	259, 261, 687, 722
SspMI	CTAG	1 cut(s)	701
StyD4I	CCNGG	1 cut(s)	272
TaaI	ACNGT	3 cut(s)	100, 127, 157
TaqI	TCGA	1 cut(s)	427
TasI	AATT	7 cut(s)	5, 10, 31, 279, 390, 415, 465
TatI	WGTACW	1 cut(s)	632
TfiI	GAWTC	4 cut(s)	328, 444, 514, 585
Tru1I	TTAA	3 cut(s)	144, 222, 228
Tru9I	TTAA	3 cut(s)	144, 222, 228
TscAI	CASTG	1 cut(s)	103
TseFI	GTSAC	1 cut(s)	247
TseI	GCWGC	2 cut(s)	51, 365
Tsp45I	GTSAC	1 cut(s)	247
TspDTI	ATGAA	6 cut(s)	252, 294, 436, 485, 548, 655
TspGWI	ACGGA	2 cut(s)	73, 682
TspRI	CASTG	1 cut(s)	103
XapI	RAATTY	2 cut(s)	10, 465
XmnI	GAANNNNTTC	1 cut(s)	513
XspI	CTAG	1 cut(s)	701

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.