Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0001267

Physical Location & Seq

Genomic Coordinates Reverse (-)

34962910 .. 34965821

Gene Structure 2912 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1217400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 825 bp

ATGAGGCCTAATTGGGAACTCACAAACTGCTGCAACCACGAGCAAGTCGTCTTCCTCATCACCGTTGCTGTCTGCACTGTCATCATACTTGCTCTTTGGAGGACAGTACTTCTGAGACCGTTCAAGCTTGTCACTGTGTTTCTTCACGAGGCGAGCCATGCTATTGCTTGTAAACTTACATGTGGTCATGTGGAAGGGATTCAAGTTCATGCAGATGAGGGCGGAACCACACAAACTCGGGGTGGTGTATACTGGTTGATTTTGCCCGCTGGATATCTTGGTTCGTCCTTTTGGGGGATGATCTTGATTCTTGCATCTACAAATCTCCTTACCACTCGAATAGCTGCTGGGTGTTTTATTGCTGCACTGCTGATTGTACTGTGCATAGCTAAAAATTGGATGCTCCGAGGACTCTGTATAGGATTCATTGTTTTTCTCGGCGTAATTTGGGTTCTGCAAGAAACAACAACAGTTCGTGTACTTCGGTACATTATTCTATTTATTGGTGTAATGAACAGCTTGTTTTCAGTCTATGATATCTATGACGATCTTATATCCCGTAGAGTTCGTTCAAGTGACGCCGAGAAATTTGCAGAAGAGTGTCCGTGCTGCACTGGTTGTGGATGGGGTGTCATCTGGGGGTTTATATCTTTCGGGTTTCTTTGCGGAGCCATGTACCTTGGTCTTGTTACCAAATCCGGTAAAAGTATTTGGTTGCTGCGAAGTTCCGACACCATGATGTCCGGTGGCCGGCAATGGTCCGTGGCTGCCATCGCCCACGAGTGCTATCTAATACCGTCAAGCAGATTTTTACGTCATTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M50B

Protein Analysis

275

Amino Acids

30.46

Weight (kDa)

8.54

Isoelectric Point (pI)

40.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M50B	PF13398	23 - 223	2.5e-57	Peptidase M50B-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001267)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67060 AT1G67060 AT1G67060
fragaria_vesca	FvH4_3g34180 FvH4_5g11892 FvH4_5g11892 FvH4_5g11892 FvH4_5g11892 FvH4_5g11892 FvH4_5g11892 FvH4_5g11892 FvH4_5g11892 FvH4_7g13690 FvH4_7g13690 FvH4_7g13690 FvH4_7g13690 FvH4_7g13690
malus_domestica	MD06G1217400.v1.1 MD06G1217500.v1.1 MD09G1253200.v1.1 MD14G1227700.v1.1
prunus_persica	Prupe.3G143000_v2.0.a1 Prupe.5G222600_v2.0.a1 Prupe.5G222600_v2.0.a1 Prupe.5G222600_v2.0.a1 Prupe.5G222600_v2.0.a1 Prupe.5G222700_v2.0.a1 Prupe.5G222700_v2.0.a1 Prupe.5G222700_v2.0.a1
pyrus_communis	pycom06g19370 pycom06g19380
rosa_chinensis	RchiOBHm_Chr2g0126081 RchiOBHm_Chr7g0185671 RchiOBHm_Chr7g0185681
rosa_multiflora	Rmu_sc0001506.1_g000004 Rmu_sc0008747.1_g000002 Rmu_sc0010190.1_g000003
rosa_roxburghii	Rroxscaffold_3G00268740
rosa_rugosa	Rorug02G0260800 Rorug02G0260900 Rorug02G0261000 Rorug06G0470300 Rorug06G0470400
rosa_samantha	Rh2BG326600 Rh2DG344700 Rh7AG072000 Rh7AG072100 Rh7BG075800 Rh7BG075900 Rh7CG075900 Rh7DG075800 Rh7DG075900
rosa_wichuraiana	Rw2G025770 Rw7G006310 Rw7G006320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	249
AciI	CCGC	3 cut(s)	222, 267, 666
AcoI	YGGCCR	1 cut(s)	748
AcsI	RAATTY	1 cut(s)	587
AcyI	GRCGYC	1 cut(s)	579
AfaI	GTAC	5 cut(s)	108, 378, 480, 488, 677
AfiI	CCNNNNNNNGG	2 cut(s)	294, 750
AflIII	ACRYGT	1 cut(s)	179
AgsI	TTSAA	3 cut(s)	124, 203, 573
AluBI	AGCT	4 cut(s)	127, 344, 389, 519
AluI	AGCT	4 cut(s)	127, 344, 389, 519
Alw26I	GTCTC	1 cut(s)	109
Ama87I	CYCGRG	1 cut(s)	237
AoxI	GGCC	2 cut(s)	5, 748
ApeKI	GCWGC	6 cut(s)	30, 344, 362, 609, 718, 767
ApoI	RAATTY	1 cut(s)	587
Asp700I	GAANNNNTTC	1 cut(s)	198
AspS9I	GGNCC	1 cut(s)	759
AsuHPI	GGTGA	1 cut(s)	52
AvaI	CYCGRG	1 cut(s)	237
AvaII	GGWCC	1 cut(s)	759
BauI	CACGAG	3 cut(s)	38, 146, 779
BbsI	GAAGAC	1 cut(s)	43
BbvI	GCAGC	6 cut(s)	17, 331, 349, 596, 705, 754
BccI	CCATC	2 cut(s)	618, 779
BcgI	CGANNNNNNTGC	2 cut(s)	572, 606
BcoDI	GTCTC	1 cut(s)	109
BisI	GCNGC	6 cut(s)	31, 345, 363, 610, 719, 768
BlsI	GCNGC	6 cut(s)	32, 346, 364, 611, 720, 769
BmcAI	AGTACT	1 cut(s)	108
Bme18I	GGWCC	1 cut(s)	759
BmeT110I	CYCGRG	1 cut(s)	237
BmgT120I	GGNCC	1 cut(s)	759
BmiI	GGNNCC	2 cut(s)	226, 670
BmsI	GCATC	2 cut(s)	323, 390
BpiI	GAAGAC	1 cut(s)	43
BsaHI	GRCGYC	1 cut(s)	579
BsaI	GGTCTC	1 cut(s)	109
BsaJI	CCNNGG	3 cut(s)	406, 679, 762
BsaWI	WCCGGW	2 cut(s)	698, 743
Bsc4I	CCNNNNNNNGG	2 cut(s)	294, 750
Bse118I	RCCGGY	1 cut(s)	750
Bse1I	ACTGG	2 cut(s)	257, 619
Bse3DI	GCAATG	1 cut(s)	761
BseDI	CCNNGG	3 cut(s)	406, 679, 762
BseGI	GGATG	3 cut(s)	303, 405, 629
BseLI	CCNNNNNNNGG	2 cut(s)	294, 750
BseMI	GCAATG	1 cut(s)	761
BseMII	CTCAG	1 cut(s)	104
BseNI	ACTGG	2 cut(s)	257, 619
BseXI	GCAGC	6 cut(s)	17, 331, 349, 596, 705, 754
BseYI	CCCAGC	1 cut(s)	347
BsgI	GTGCAG	3 cut(s)	58, 348, 595
BshFI	GGCC	2 cut(s)	7, 750
BsiHKCI	CYCGRG	1 cut(s)	237
BsiSI	CCGG	3 cut(s)	699, 744, 751
BslI	CCNNNNNNNGG	2 cut(s)	294, 750
BsmAI	GTCTC	1 cut(s)	109
BsnI	GGCC	2 cut(s)	7, 750
Bso31I	GGTCTC	1 cut(s)	109
BsoBI	CYCGRG	1 cut(s)	237
Bsp143I	GATC	2 cut(s)	300, 547
BspACI	CCGC	3 cut(s)	222, 267, 666
BspANI	GGCC	2 cut(s)	7, 750
BspCNI	CTCAG	1 cut(s)	105
BspLI	GGNNCC	2 cut(s)	226, 670
BspTNI	GGTCTC	1 cut(s)	109
BsrDI	GCAATG	1 cut(s)	761
BsrFI	RCCGGY	1 cut(s)	750
BsrI	ACTGG	2 cut(s)	257, 619
BssAI	RCCGGY	1 cut(s)	750
BssECI	CCNNGG	3 cut(s)	406, 679, 762
BssMI	GATC	2 cut(s)	300, 547
BssNAI	GTATAC	1 cut(s)	250
BssNI	GRCGYC	1 cut(s)	579
BssSI	CACGAG	3 cut(s)	38, 146, 779
BssT1I	CCWWGG	1 cut(s)	679
Bst1107I	GTATAC	1 cut(s)	250
Bst2BI	CACGAG	3 cut(s)	38, 146, 779
Bst4CI	ACNGT	8 cut(s)	64, 79, 106, 120, 136, 381, 472, 798
Bst6I	CTCTTC	1 cut(s)	591
BstACI	GRCGYC	1 cut(s)	579
BstC8I	GCNNGC	3 cut(s)	154, 267, 752
BstDEI	CTNAG	2 cut(s)	113, 822
BstDSI	CCRYGG	1 cut(s)	762
BstF5I	GGATG	3 cut(s)	303, 405, 629
BstKTI	GATC	2 cut(s)	303, 550
BstMAI	GTCTC	1 cut(s)	109
BstMBI	GATC	2 cut(s)	300, 547
BstMWI	GCNNNNNNNGC	2 cut(s)	158, 773
BstNSI	RCATGY	1 cut(s)	183
BstV1I	GCAGC	6 cut(s)	17, 331, 349, 596, 705, 754
BstV2I	GAAGAC	1 cut(s)	43
BstZ17I	GTATAC	1 cut(s)	250
BsuRI	GGCC	2 cut(s)	7, 750
BtgI	CCRYGG	1 cut(s)	762
BtgZI	GCGATG	1 cut(s)	757
BtsCI	GGATG	3 cut(s)	303, 405, 629
BtsI	GCAGTG	1 cut(s)	365
BtsIMutI	CAGTG	4 cut(s)	75, 132, 365, 612
Cac8I	GCNNGC	3 cut(s)	154, 267, 752
Cfr10I	RCCGGY	1 cut(s)	750
Cfr13I	GGNCC	1 cut(s)	759
CseI	GACGC	1 cut(s)	587
Csp6I	GTAC	5 cut(s)	107, 377, 479, 487, 676
CviAII	CATG	6 cut(s)	158, 180, 188, 209, 673, 736
CviJI	RGCY	9 cut(s)	7, 127, 156, 344, 389, 519, 671, 750, 767
CviKI_1	RGCY	9 cut(s)	7, 127, 156, 344, 389, 519, 671, 750, 767
CviQI	GTAC	5 cut(s)	107, 377, 479, 487, 676
DdeI	CTNAG	2 cut(s)	113, 822
DpnI	GATC	2 cut(s)	302, 549
DpnII	GATC	2 cut(s)	300, 547
EaeI	YGGCCR	1 cut(s)	748
Eam1104I	CTCTTC	1 cut(s)	591
EarI	CTCTTC	1 cut(s)	591
EciI	GGCGGA	1 cut(s)	237
Eco130I	CCWWGG	1 cut(s)	679
Eco147I	AGGCCT	1 cut(s)	7
Eco31I	GGTCTC	1 cut(s)	109
Eco32I	GATATC	2 cut(s)	275, 538
Eco47I	GGWCC	1 cut(s)	759
Eco88I	CYCGRG	1 cut(s)	237
EcoRV	GATATC	2 cut(s)	275, 538
EcoT14I	CCWWGG	1 cut(s)	679
ErhI	CCWWGG	1 cut(s)	679
FaeI	CATG	6 cut(s)	161, 183, 191, 212, 676, 739
FatI	CATG	6 cut(s)	157, 179, 187, 208, 672, 735
FauI	CCCGC	1 cut(s)	274
FblI	GTMKAC	1 cut(s)	249
Fnu4HI	GCNGC	6 cut(s)	31, 345, 363, 610, 719, 768
FokI	GGATG	3 cut(s)	310, 412, 636
Fsp4HI	GCNGC	6 cut(s)	31, 345, 363, 610, 719, 768
GluI	GCNGC	6 cut(s)	31, 345, 363, 610, 719, 768
GsaI	CCCAGC	1 cut(s)	351
HaeIII	GGCC	2 cut(s)	7, 750
HapII	CCGG	3 cut(s)	699, 744, 751
HgaI	GACGC	1 cut(s)	587
Hin1I	GRCGYC	1 cut(s)	579
Hin1II	CATG	6 cut(s)	161, 183, 191, 212, 676, 739
HindIII	AAGCTT	1 cut(s)	125
HinfI	GANTC	4 cut(s)	199, 307, 411, 423
HpaII	CCGG	3 cut(s)	699, 744, 751
HphI	GGTGA	1 cut(s)	52
Hpy166II	GTNNAC	3 cut(s)	173, 250, 479
Hpy188I	TCNGA	3 cut(s)	114, 407, 730
Hpy188III	TCNNGA	2 cut(s)	146, 304
Hpy8I	GTNNAC	3 cut(s)	173, 250, 479
HpyAV	CCTTC	1 cut(s)	188
HpyCH4III	ACNGT	8 cut(s)	64, 79, 106, 120, 136, 381, 472, 798
HpyCH4IV	ACGT	1 cut(s)	814
HpyCH4V	TGCA	9 cut(s)	33, 75, 212, 314, 365, 384, 457, 593, 612
HpyF10VI	GCNNNNNNNGC	2 cut(s)	158, 773
HpyF3I	CTNAG	2 cut(s)	113, 822
HpySE526I	ACGT	1 cut(s)	814
Hsp92I	GRCGYC	1 cut(s)	579
Hsp92II	CATG	6 cut(s)	161, 183, 191, 212, 676, 739
KroI	GCCGGC	1 cut(s)	750
KroNI	GCCGGC	1 cut(s)	752
Kzo9I	GATC	2 cut(s)	300, 547
LmnI	GCTCC	2 cut(s)	408, 668
LpnPI	CCDG	8 cut(s)	238, 255, 333, 600, 622, 712, 757, 764
Lsp1109I	GCAGC	6 cut(s)	17, 331, 349, 596, 705, 754
LweI	GCATC	2 cut(s)	323, 390
MaeII	ACGT	1 cut(s)	814
MaeIII	GTNAC	3 cut(s)	130, 575, 688
MalI	GATC	2 cut(s)	302, 549
MboI	GATC	2 cut(s)	300, 547
MboII	GAAGA	3 cut(s)	43, 134, 608
MluCI	AATT	4 cut(s)	10, 394, 444, 587
MlyI	GAGTC	1 cut(s)	405
MmeI	TCCRAC	1 cut(s)	753
MnlI	CCTC	5 cut(s)	65, 93, 142, 211, 401
MroNI	GCCGGC	1 cut(s)	750
MroXI	GAANNNNTTC	1 cut(s)	198
MslI	CAYNNNNRTG	2 cut(s)	213, 737
MspA1I	CMGCKG	1 cut(s)	269
MspI	CCGG	3 cut(s)	699, 744, 751
MwoI	GCNNNNNNNGC	2 cut(s)	158, 773
NaeI	GCCGGC	1 cut(s)	752
NdeII	GATC	2 cut(s)	300, 547
NgoMIV	GCCGGC	1 cut(s)	750
NlaIII	CATG	6 cut(s)	161, 183, 191, 212, 676, 739
NlaIV	GGNNCC	2 cut(s)	226, 670
NmeAIII	GCCGAG	2 cut(s)	417, 607
NmuCI	GTSAC	2 cut(s)	130, 575
NspI	RCATGY	1 cut(s)	183
PceI	AGGCCT	1 cut(s)	7
PciI	ACATGT	1 cut(s)	179
PcsI	WCGNNNNNNNCGW	1 cut(s)	45
PdiI	GCCGGC	1 cut(s)	752
PdmI	GAANNNNTTC	1 cut(s)	198
PfeI	GAWTC	3 cut(s)	199, 307, 423
PkrI	GCNGC	6 cut(s)	32, 346, 364, 611, 720, 769
PleI	GAGTC	1 cut(s)	405
PpsI	GAGTC	1 cut(s)	405
PscI	ACATGT	1 cut(s)	179
PspFI	CCCAGC	1 cut(s)	347
PspN4I	GGNNCC	2 cut(s)	226, 670
PspPI	GGNCC	1 cut(s)	759
PsrI	GAACNNNNNNTAC	4 cut(s)	435, 467, 553, 585
RsaI	GTAC	5 cut(s)	108, 378, 480, 488, 677
RsaNI	GTAC	5 cut(s)	107, 377, 479, 487, 676
RseI	CAYNNNNRTG	2 cut(s)	213, 737
SatI	GCNGC	6 cut(s)	31, 345, 363, 610, 719, 768
Sau3AI	GATC	2 cut(s)	300, 547
Sau96I	GGNCC	1 cut(s)	759
ScaI	AGTACT	1 cut(s)	108
SchI	GAGTC	1 cut(s)	405
SetI	ASST	6 cut(s)	129, 346, 391, 521, 681, 817
SfaNI	GCATC	2 cut(s)	323, 390
SinI	GGWCC	1 cut(s)	759
SmiMI	CAYNNNNRTG	2 cut(s)	213, 737
Sse9I	AATT	4 cut(s)	10, 394, 444, 587
SseBI	AGGCCT	1 cut(s)	7
SsiI	CCGC	3 cut(s)	222, 267, 666
StuI	AGGCCT	1 cut(s)	7
StyI	CCWWGG	1 cut(s)	679
TaaI	ACNGT	8 cut(s)	64, 79, 106, 120, 136, 381, 472, 798
TaiI	ACGT	1 cut(s)	817
TaqI	TCGA	1 cut(s)	337
TasI	AATT	4 cut(s)	10, 394, 444, 587
TatI	WGTACW	3 cut(s)	106, 376, 478
TfiI	GAWTC	3 cut(s)	199, 307, 423
TscAI	CASTG	4 cut(s)	82, 139, 372, 619
TseFI	GTSAC	2 cut(s)	130, 575
TseI	GCWGC	6 cut(s)	30, 344, 362, 609, 718, 767
Tsp45I	GTSAC	2 cut(s)	130, 575
TspDTI	ATGAA	3 cut(s)	197, 415, 527
TspGWI	ACGGA	2 cut(s)	594, 751
TspRI	CASTG	4 cut(s)	82, 139, 372, 619
VpaK11BI	GGWCC	1 cut(s)	759
XapI	RAATTY	1 cut(s)	587
XceI	RCATGY	1 cut(s)	183
XmiI	GTMKAC	1 cut(s)	249
XmnI	GAANNNNTTC	1 cut(s)	198
ZrmI	AGTACT	1 cut(s)	108

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.