Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0001267

Physical Location & Seq

Genomic Coordinates Reverse (-)

34967483 .. 34969593

Gene Structure 2111 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1217500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 738 bp

ATGGAGTTGAATTTGAATTTCATGAAATGGAGGCCAAATTGGCAACTCAGAAAATGCTGCAACCACGACCAAGTAATCTTCCTCATCTCCGTTGCTGTCTGCACTGTTGTTATACTTGTGCTGTGGAGGACAGTGCTGATGACACCTTTTAAGCTTATAACCGTGTTTCTTCACGAGGTGAGCCATGCTATCGCTTGTTTGCTTACATGTGGTAAGGTGGAAGGGATTAAAGTTCAGGCAGATGAAGGTGGAGTCACACAAACCCGCGGTGGCATATCCTGGATAATTTTGCCAGCCGGATATCTTGGTTCATCGTTTTGGGGGATGATATTGATTCTTGCATCTACGAAGCATCTCACTACACAAATCGCTGCTGGCTGTTTCATCGTTGCCCTAATTATTGTATTCTTTTTAGCTAAAAATTGGACGCTTCGAGGACTTTGCATCGGATTCATTGTTTTATTTGCTGCAATTTGGTATCTGCAAGAAGCAACGCCGGTTCATATGCTTAGGGAAGTGATTCTGTTCACCGGTGTGATGAACAGCTTGTTTTCGGTGTATGATATATACGATGATCTTATATCGCGTAGAATTAATACGAGTGATGCTGAAAGATTTGCAGATGAGTGTCCCTGCTGTACTGGATGTGGATGGGGTGTCATTTGGGCGTTCATATCTTTCACATATCTCTGTGGATCTGTGTACCTTGCTCTTGTGATCTTGTCTCCACGTCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M50B

Protein Analysis

246

Amino Acids

27.48

Weight (kDa)

7.97

Isoelectric Point (pI)

28.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M50B	PF13398	32 - 232	5.8e-55	Peptidase M50B-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0001267)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67060 AT1G67060 AT1G67060
fragaria_vesca	FvH4_3g34180 FvH4_5g11892 FvH4_5g11892 FvH4_5g11892 FvH4_5g11892 FvH4_5g11892 FvH4_5g11892 FvH4_5g11892 FvH4_5g11892 FvH4_7g13690 FvH4_7g13690 FvH4_7g13690 FvH4_7g13690 FvH4_7g13690
malus_domestica	MD06G1217400.v1.1 MD06G1217500.v1.1 MD09G1253200.v1.1 MD14G1227700.v1.1
prunus_persica	Prupe.3G143000_v2.0.a1 Prupe.5G222600_v2.0.a1 Prupe.5G222600_v2.0.a1 Prupe.5G222600_v2.0.a1 Prupe.5G222600_v2.0.a1 Prupe.5G222700_v2.0.a1 Prupe.5G222700_v2.0.a1 Prupe.5G222700_v2.0.a1
pyrus_communis	pycom06g19370 pycom06g19380
rosa_chinensis	RchiOBHm_Chr2g0126081 RchiOBHm_Chr7g0185671 RchiOBHm_Chr7g0185681
rosa_multiflora	Rmu_sc0001506.1_g000004 Rmu_sc0008747.1_g000002 Rmu_sc0010190.1_g000003
rosa_roxburghii	Rroxscaffold_3G00268740
rosa_rugosa	Rorug02G0260800 Rorug02G0260900 Rorug02G0261000 Rorug06G0470300 Rorug06G0470400
rosa_samantha	Rh2BG326600 Rh2DG344700 Rh7AG072000 Rh7AG072100 Rh7BG075800 Rh7BG075900 Rh7CG075900 Rh7DG075800 Rh7DG075900
rosa_wichuraiana	Rw2G025770 Rw7G006310 Rw7G006320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	158
AccII	CGCG	2 cut(s)	267, 586
AciI	CCGC	2 cut(s)	265, 267
AclWI	GGATC	1 cut(s)	703
AcsI	RAATTY	2 cut(s)	10, 16
AdeI	CACNNNGTG	1 cut(s)	178
AfaI	GTAC	2 cut(s)	640, 704
AflIII	ACRYGT	1 cut(s)	206
AgeI	ACCGGT	1 cut(s)	530
AgsI	TTSAA	2 cut(s)	10, 16
AjiI	CACGTC	1 cut(s)	731
AjnI	CCWGG	1 cut(s)	278
AleI	CACNNNNGTG	2 cut(s)	533, 733
AluBI	AGCT	3 cut(s)	154, 416, 546
AluI	AGCT	3 cut(s)	154, 416, 546
Alw26I	GTCTC	1 cut(s)	729
AlwI	GGATC	1 cut(s)	703
AoxI	GGCC	1 cut(s)	32
ApeKI	GCWGC	3 cut(s)	57, 371, 467
ApoI	RAATTY	2 cut(s)	10, 16
AseI	ATTAAT	1 cut(s)	594
AsiGI	ACCGGT	1 cut(s)	530
Asp700I	GAANNNNTTC	1 cut(s)	519
AsuHPI	GGTGA	2 cut(s)	190, 520
BauI	CACGAG	1 cut(s)	173
BbvI	GCAGC	3 cut(s)	44, 358, 454
BccI	CCATC	1 cut(s)	645
BcgI	CGANNNNNNTGC	2 cut(s)	423, 457
BciT130I	CCWGG	1 cut(s)	280
BcoDI	GTCTC	1 cut(s)	729
BglI	GCCNNNNNGGC	1 cut(s)	40
BisI	GCNGC	3 cut(s)	58, 372, 468
BlsI	GCNGC	3 cut(s)	59, 373, 469
Bme1390I	CCNGG	1 cut(s)	280
BmgBI	CACGTC	1 cut(s)	731
BmrFI	CCNGG	1 cut(s)	280
BmsI	GCATC	4 cut(s)	350, 361, 453, 595
Bpu10I	CCTNAGC	1 cut(s)	509
BsaJI	CCNNGG	1 cut(s)	265
BsaWI	WCCGGW	1 cut(s)	530
Bse118I	RCCGGY	2 cut(s)	496, 530
Bse1I	ACTGG	1 cut(s)	646
BseBI	CCWGG	1 cut(s)	280
BseDI	CCNNGG	1 cut(s)	265
BseGI	GGATG	3 cut(s)	330, 650, 656
BseMII	CTCAG	1 cut(s)	61
BseNI	ACTGG	1 cut(s)	646
BseXI	GCAGC	3 cut(s)	44, 358, 454
BsgI	GTGCAG	1 cut(s)	85
Bsh1236I	CGCG	2 cut(s)	267, 586
BshFI	GGCC	1 cut(s)	34
BshTI	ACCGGT	1 cut(s)	530
BsiSI	CCGG	3 cut(s)	297, 497, 531
BslFI	GGGAC	1 cut(s)	615
BsmAI	GTCTC	1 cut(s)	729
BsmFI	GGGAC	1 cut(s)	615
BsnI	GGCC	1 cut(s)	34
Bsp143I	GATC	3 cut(s)	574, 695, 717
BspACI	CCGC	2 cut(s)	265, 267
BspANI	GGCC	1 cut(s)	34
BspCNI	CTCAG	1 cut(s)	60
BspFNI	CGCG	2 cut(s)	267, 586
BspHI	TCATGA	1 cut(s)	21
BspPI	GGATC	1 cut(s)	703
BsrFI	RCCGGY	2 cut(s)	496, 530
BsrI	ACTGG	1 cut(s)	646
BssAI	RCCGGY	2 cut(s)	496, 530
BssECI	CCNNGG	1 cut(s)	265
BssMI	GATC	3 cut(s)	574, 695, 717
BssSI	CACGAG	1 cut(s)	173
Bst2BI	CACGAG	1 cut(s)	173
Bst2UI	CCWGG	1 cut(s)	280
Bst4CI	ACNGT	3 cut(s)	106, 133, 163
BstC8I	GCNNGC	2 cut(s)	294, 376
BstDEI	CTNAG	2 cut(s)	47, 509
BstDSI	CCRYGG	1 cut(s)	265
BstF5I	GGATG	3 cut(s)	330, 650, 656
BstFNI	CGCG	2 cut(s)	267, 586
BstKTI	GATC	3 cut(s)	577, 698, 720
BstMAI	GTCTC	1 cut(s)	729
BstMBI	GATC	3 cut(s)	574, 695, 717
BstMWI	GCNNNNNNNGC	1 cut(s)	40
BstNI	CCWGG	1 cut(s)	280
BstNSI	RCATGY	1 cut(s)	210
BstSCI	CCNGG	1 cut(s)	278
BstUI	CGCG	2 cut(s)	267, 586
BstV1I	GCAGC	3 cut(s)	44, 358, 454
BstX2I	RGATCY	1 cut(s)	695
BstYI	RGATCY	1 cut(s)	695
BsuRI	GGCC	1 cut(s)	34
BtgI	CCRYGG	1 cut(s)	265
BtrI	CACGTC	1 cut(s)	731
BtsCI	GGATG	3 cut(s)	330, 650, 656
BtsIMutI	CAGTG	2 cut(s)	102, 138
Cac8I	GCNNGC	2 cut(s)	294, 376
CciI	TCATGA	1 cut(s)	21
Cfr10I	RCCGGY	2 cut(s)	496, 530
Cfr42I	CCGCGG	1 cut(s)	268
CseI	GACGC	1 cut(s)	436
Csp6I	GTAC	2 cut(s)	639, 703
CspAI	ACCGGT	1 cut(s)	530
CviAII	CATG	3 cut(s)	22, 185, 207
CviJI	RGCY	7 cut(s)	34, 154, 183, 296, 378, 416, 546
CviKI_1	RGCY	7 cut(s)	34, 154, 183, 296, 378, 416, 546
CviQI	GTAC	2 cut(s)	639, 703
DdeI	CTNAG	2 cut(s)	47, 509
DpnI	GATC	3 cut(s)	576, 697, 719
DpnII	GATC	3 cut(s)	574, 695, 717
DraIII	CACNNNGTG	1 cut(s)	178
Eco32I	GATATC	1 cut(s)	302
EcoRII	CCWGG	1 cut(s)	278
EcoRV	GATATC	1 cut(s)	302
FaeI	CATG	3 cut(s)	25, 188, 210
FaqI	GGGAC	1 cut(s)	615
FatI	CATG	3 cut(s)	21, 184, 206
FauI	CCCGC	1 cut(s)	272
FauNDI	CATATG	1 cut(s)	504
Fnu4HI	GCNGC	3 cut(s)	58, 372, 468
FokI	GGATG	3 cut(s)	337, 657, 663
Fsp4HI	GCNGC	3 cut(s)	58, 372, 468
GluI	GCNGC	3 cut(s)	58, 372, 468
HaeIII	GGCC	1 cut(s)	34
HapII	CCGG	3 cut(s)	297, 497, 531
HgaI	GACGC	1 cut(s)	436
Hin1II	CATG	3 cut(s)	25, 188, 210
HindIII	AAGCTT	1 cut(s)	152
HinfI	GANTC	4 cut(s)	252, 334, 450, 520
HpaII	CCGG	3 cut(s)	297, 497, 531
HphI	GGTGA	2 cut(s)	190, 520
Hpy166II	GTNNAC	2 cut(s)	528, 703
Hpy188I	TCNGA	2 cut(s)	50, 449
Hpy188III	TCNNGA	2 cut(s)	22, 173
Hpy8I	GTNNAC	2 cut(s)	528, 703
HpyAV	CCTTC	2 cut(s)	215, 239
HpyCH4III	ACNGT	3 cut(s)	106, 133, 163
HpyCH4IV	ACGT	1 cut(s)	730
HpyCH4V	TGCA	7 cut(s)	60, 102, 341, 444, 470, 484, 620
HpyF10VI	GCNNNNNNNGC	1 cut(s)	40
HpyF3I	CTNAG	2 cut(s)	47, 509
HpySE526I	ACGT	1 cut(s)	730
Hsp92II	CATG	3 cut(s)	25, 188, 210
KspI	CCGCGG	1 cut(s)	268
Kzo9I	GATC	3 cut(s)	574, 695, 717
Lsp1109I	GCAGC	3 cut(s)	44, 358, 454
LweI	GCATC	4 cut(s)	350, 361, 453, 595
MaeII	ACGT	1 cut(s)	730
MaeIII	GTNAC	1 cut(s)	253
MalI	GATC	3 cut(s)	576, 697, 719
MboI	GATC	3 cut(s)	574, 695, 717
MboII	GAAGA	2 cut(s)	70, 161
MflI	RGATCY	1 cut(s)	695
MluCI	AATT	8 cut(s)	10, 16, 37, 285, 396, 421, 471, 591
MlyI	GAGTC	1 cut(s)	261
MnlI	CCTC	5 cut(s)	24, 92, 120, 169, 428
MroXI	GAANNNNTTC	1 cut(s)	519
MseI	TTAA	3 cut(s)	150, 228, 594
MslI	CAYNNNNRTG	2 cut(s)	533, 733
MspA1I	CMGCKG	1 cut(s)	267
MspI	CCGG	3 cut(s)	297, 497, 531
MspR9I	CCNGG	1 cut(s)	280
MvaI	CCWGG	1 cut(s)	280
MvnI	CGCG	2 cut(s)	267, 586
MwoI	GCNNNNNNNGC	1 cut(s)	40
NdeI	CATATG	1 cut(s)	504
NdeII	GATC	3 cut(s)	574, 695, 717
NlaIII	CATG	3 cut(s)	25, 188, 210
NmuCI	GTSAC	1 cut(s)	253
NspI	RCATGY	1 cut(s)	210
OliI	CACNNNNGTG	2 cut(s)	533, 733
PagI	TCATGA	1 cut(s)	21
PciI	ACATGT	1 cut(s)	206
PdmI	GAANNNNTTC	1 cut(s)	519
PfeI	GAWTC	3 cut(s)	334, 450, 520
PfoI	TCCNGGA	1 cut(s)	278
PinAI	ACCGGT	1 cut(s)	530
PkrI	GCNGC	3 cut(s)	59, 373, 469
PleI	GAGTC	1 cut(s)	260
PpsI	GAGTC	1 cut(s)	260
PscI	ACATGT	1 cut(s)	206
PshBI	ATTAAT	1 cut(s)	594
PsiI	TTATAA	1 cut(s)	158
Psp6I	CCWGG	1 cut(s)	278
PspGI	CCWGG	1 cut(s)	278
PsuI	RGATCY	1 cut(s)	695
RsaI	GTAC	2 cut(s)	640, 704
RsaNI	GTAC	2 cut(s)	639, 703
RseI	CAYNNNNRTG	2 cut(s)	533, 733
SacII	CCGCGG	1 cut(s)	268
SaqAI	TTAA	3 cut(s)	150, 228, 594
SatI	GCNGC	3 cut(s)	58, 372, 468
Sau3AI	GATC	3 cut(s)	574, 695, 717
SchI	GAGTC	1 cut(s)	261
ScrFI	CCNGG	1 cut(s)	280
SetI	ASST	9 cut(s)	148, 156, 180, 219, 250, 418, 548, 708, 733
SfaNI	GCATC	4 cut(s)	350, 361, 453, 595
Sfr303I	CCGCGG	1 cut(s)	268
SgrAI	CRCCGGYG	1 cut(s)	530
SgrBI	CCGCGG	1 cut(s)	268
SmiMI	CAYNNNNRTG	2 cut(s)	533, 733
Sse9I	AATT	8 cut(s)	10, 16, 37, 285, 396, 421, 471, 591
SsiI	CCGC	2 cut(s)	265, 267
StyD4I	CCNGG	1 cut(s)	278
TaaI	ACNGT	3 cut(s)	106, 133, 163
TaiI	ACGT	1 cut(s)	733
TaqI	TCGA	1 cut(s)	433
TasI	AATT	8 cut(s)	10, 16, 37, 285, 396, 421, 471, 591
TatI	WGTACW	1 cut(s)	638
TfiI	GAWTC	3 cut(s)	334, 450, 520
Tru1I	TTAA	3 cut(s)	150, 228, 594
Tru9I	TTAA	3 cut(s)	150, 228, 594
TscAI	CASTG	2 cut(s)	109, 138
TseFI	GTSAC	1 cut(s)	253
TseI	GCWGC	3 cut(s)	57, 371, 467
Tsp45I	GTSAC	1 cut(s)	253
TspDTI	ATGAA	9 cut(s)	10, 38, 258, 300, 373, 442, 491, 554, 661
TspGWI	ACGGA	1 cut(s)	79
TspRI	CASTG	2 cut(s)	109, 138
VspI	ATTAAT	1 cut(s)	594
XapI	RAATTY	2 cut(s)	10, 16
XceI	RCATGY	1 cut(s)	210
XmnI	GAANNNNTTC	1 cut(s)	519

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.