Rorug06G0470400

Peptidase M50B-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001267
Physical Location & Seq
Forward (+)
59388953 .. 59391263
2311 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0470400.1

Sequence Viewer

Length: 807 bp
ATGGCCGCCATATATATAGATGCTAAATCCCTCGGTCAATCTTCAGTCTCTATCTCGAGAACTTCTCTTCAGCCGCTCTCTCTCTTGTCTCGCCCTCTCATTCTTCTCTTCGTCAATCCTTTCAGTTTTCTCATCGCCATAGAGAATTCGAGATCCCCAAGGACTAGATCGATCAAGCTCACGAGGCTAAGGTCTCGGTTGGGTCATGAAGACCAGATCGATGGCCAGGGATCTGAGCGAGATAGTGAAGGAGATTCTCTCGGTTGGGTGCTAGTACCAATCTTTATATTCGTCAATGATACATCTTCTGCACCTTGGTATCTCAATGCCGAAAGACCTATCCAGATTGCTGTTGAAACTGATGATGTATTCAAAAGTGCTTGCTGCTTAGCTTTTTCTAGACCAAGATGGTTGGAAAACTTATCAAAATTGTCAGATTCTTTTATGTTGGTGGAAGCTGCTTTAGCAGAGCATGAAATTTCTGGGTTAAATTTTCTCTCTATCAACCGTTTTGAGAGGAAAAAGAATATAGACTTGGCGATTTCAGCTTTTGCCATGCTTCGCACCCTTGAAGGAGATGTGCTTCATGGTCTTAATCTAGCTGAAGCTTCCTTGACAATTGTAGTTCAATCTAGTGCTGCAAGCTGGTCTTGGTACGGAGATGTTGACTTGGGAGAGAATCTGCAAGCTCAAGCTCCAATGGCCTTTATTATTTTTGAAAGGAAGGCTCCAATGGCCTTTATTCTTAATTTCCGGATTATAGATGGGATTGATTCGTGGCTCAAATGGATATATATAAATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M50B

Protein Analysis

268

Amino Acids

30.03

Weight (kDa)

5.21

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 76
AccIII TCCGGA 1 cut(s) 753
AciI CCGC 2 cut(s) 6, 74
AclWI GGATC 2 cut(s) 147, 238
AcoI YGGCCR 2 cut(s) 3, 223
AcsI RAATTY 3 cut(s) 145, 477, 490
AcuI CTGAAG 3 cut(s) 27, 53, 624
AfaI GTAC 2 cut(s) 276, 656
AgsI TTSAA 5 cut(s) 356, 373, 572, 629, 719
AjnI CCWGG 1 cut(s) 225
AjuI GAANNNNNNNTTGG 2 cut(s) 518, 550
AluBI AGCT 9 cut(s) 178, 392, 458, 548, 602, 608, 645, 689, 695
AluI AGCT 9 cut(s) 178, 392, 458, 548, 602, 608, 645, 689, 695
Alw26I GTCTC 3 cut(s) 52, 93, 198
AlwI GGATC 2 cut(s) 147, 238
Ama87I CYCGRG 1 cut(s) 55
Aor13HI TCCGGA 1 cut(s) 753
AoxI GGCC 4 cut(s) 3, 223, 702, 735
ApeKI GCWGC 3 cut(s) 384, 458, 638
ApoI RAATTY 3 cut(s) 145, 477, 490
AvaI CYCGRG 1 cut(s) 55
BaeI ACNNNNGTAYC 2 cut(s) 302, 335
BalI TGGCCA 1 cut(s) 225
BauI CACGAG 1 cut(s) 181
BbsI GAAGAC 1 cut(s) 216
BbvI GCAGC 3 cut(s) 371, 445, 625
BccI CCATC 3 cut(s) 215, 402, 758
BciT130I CCWGG 1 cut(s) 227
BcoDI GTCTC 3 cut(s) 52, 93, 198
BfaI CTAG 5 cut(s) 165, 272, 399, 599, 633
BisI GCNGC 5 cut(s) 6, 74, 385, 459, 639
BlpI GCTNAGC 1 cut(s) 388
BlsI GCNGC 5 cut(s) 7, 75, 386, 460, 640
Bme1390I CCNGG 1 cut(s) 227
BmeT110I CYCGRG 1 cut(s) 55
BmiI GGNNCC 1 cut(s) 729
BmrFI CCNGG 1 cut(s) 227
BmsI GCATC 1 cut(s) 10
BpiI GAAGAC 1 cut(s) 216
BplI GAGNNNNNCTC 4 cut(s) 49, 81, 243, 275
Bpu10I CCTNAGC 1 cut(s) 188
Bpu1102I GCTNAGC 1 cut(s) 388
BpuEI CTTGAG 1 cut(s) 675
Bsa29I ATCGAT 2 cut(s) 170, 219
BsaI GGTCTC 1 cut(s) 198
BsaJI CCNNGG 4 cut(s) 31, 158, 226, 314
BsaWI WCCGGW 1 cut(s) 753
BseAI TCCGGA 1 cut(s) 753
BseBI CCWGG 1 cut(s) 227
BseCI ATCGAT 2 cut(s) 170, 219
BseDI CCNNGG 4 cut(s) 31, 158, 226, 314
BseMII CTCAG 1 cut(s) 225
BseXI GCAGC 3 cut(s) 371, 445, 625
BsgI GTGCAG 1 cut(s) 294
BshFI GGCC 4 cut(s) 5, 225, 704, 737
BshVI ATCGAT 2 cut(s) 170, 219
BsiHKCI CYCGRG 1 cut(s) 55
BsiSI CCGG 1 cut(s) 754
BsmAI GTCTC 3 cut(s) 52, 93, 198
BsnI GGCC 4 cut(s) 5, 225, 704, 737
Bso31I GGTCTC 1 cut(s) 198
BsoBI CYCGRG 1 cut(s) 55
Bsp13I TCCGGA 1 cut(s) 753
Bsp143I GATC 5 cut(s) 152, 167, 171, 216, 230
Bsp1720I GCTNAGC 1 cut(s) 388
BspACI CCGC 2 cut(s) 6, 74
BspANI GGCC 4 cut(s) 5, 225, 704, 737
BspCNI CTCAG 1 cut(s) 226
BspDI ATCGAT 2 cut(s) 170, 219
BspEI TCCGGA 1 cut(s) 753
BspHI TCATGA 1 cut(s) 205
BspLI GGNNCC 1 cut(s) 729
BspPI GGATC 2 cut(s) 147, 238
BspTNI GGTCTC 1 cut(s) 198
BsrBI CCGCTC 1 cut(s) 76
BssECI CCNNGG 4 cut(s) 31, 158, 226, 314
BssMI GATC 5 cut(s) 152, 167, 171, 216, 230
BssSI CACGAG 1 cut(s) 181
BssT1I CCWWGG 2 cut(s) 158, 314
Bst2BI CACGAG 1 cut(s) 181
Bst2UI CCWGG 1 cut(s) 227
Bst4CI ACNGT 1 cut(s) 509
Bst6I CTCTTC 2 cut(s) 72, 113
BstC8I GCNNGC 3 cut(s) 382, 643, 687
BstDEI CTNAG 3 cut(s) 188, 234, 388
BstKTI GATC 5 cut(s) 155, 170, 174, 219, 233
BstMAI GTCTC 3 cut(s) 52, 93, 198
BstMBI GATC 5 cut(s) 152, 167, 171, 216, 230
BstMWI GCNNNNNNNGC 5 cut(s) 184, 464, 545, 701, 734
BstNI CCWGG 1 cut(s) 227
BstSCI CCNGG 1 cut(s) 225
BstV1I GCAGC 3 cut(s) 371, 445, 625
BstV2I GAAGAC 1 cut(s) 216
BstX2I RGATCY 2 cut(s) 152, 230
BstXI CCANNNNNNTGG 1 cut(s) 221
BstYI RGATCY 2 cut(s) 152, 230
Bsu15I ATCGAT 2 cut(s) 170, 219
BsuRI GGCC 4 cut(s) 5, 225, 704, 737
BsuTUI ATCGAT 2 cut(s) 170, 219
BtgZI GCGATG 1 cut(s) 118
Cac8I GCNNGC 3 cut(s) 382, 643, 687
CciI TCATGA 1 cut(s) 205
ClaI ATCGAT 2 cut(s) 170, 219
Csp6I GTAC 2 cut(s) 275, 655
CviAII CATG 4 cut(s) 206, 473, 556, 587
CviQI GTAC 2 cut(s) 275, 655
DdeI CTNAG 3 cut(s) 188, 234, 388
DpnI GATC 5 cut(s) 154, 169, 173, 218, 232
DpnII GATC 5 cut(s) 152, 167, 171, 216, 230
EaeI YGGCCR 2 cut(s) 3, 223
Eam1104I CTCTTC 2 cut(s) 72, 113
EarI CTCTTC 2 cut(s) 72, 113
Eco130I CCWWGG 2 cut(s) 158, 314
Eco31I GGTCTC 1 cut(s) 198
Eco57I CTGAAG 3 cut(s) 27, 53, 624
Eco88I CYCGRG 1 cut(s) 55
EcoRI GAATTC 1 cut(s) 145
EcoRII CCWGG 1 cut(s) 225
EcoT14I CCWWGG 2 cut(s) 158, 314
ErhI CCWWGG 2 cut(s) 158, 314
FaeI CATG 4 cut(s) 209, 476, 559, 590
FalI AAGNNNNNCTT 2 cut(s) 634, 666
FatI CATG 4 cut(s) 205, 472, 555, 586
Fnu4HI GCNGC 5 cut(s) 6, 74, 385, 459, 639
Fsp4HI GCNGC 5 cut(s) 6, 74, 385, 459, 639
FspBI CTAG 5 cut(s) 165, 272, 399, 599, 633
GluI GCNGC 5 cut(s) 6, 74, 385, 459, 639
HaeIII GGCC 4 cut(s) 5, 225, 704, 737
HapII CCGG 1 cut(s) 754
Hin1II CATG 4 cut(s) 209, 476, 559, 590
HincII GTYRAC 1 cut(s) 667
HindII GTYRAC 1 cut(s) 667
HindIII AAGCTT 1 cut(s) 606
HinfI GANTC 4 cut(s) 254, 437, 679, 773
HpaII CCGG 1 cut(s) 754
Hpy166II GTNNAC 1 cut(s) 667
Hpy188I TCNGA 2 cut(s) 235, 436
Hpy188III TCNNGA 8 cut(s) 55, 57, 150, 181, 206, 343, 399, 754
Hpy8I GTNNAC 1 cut(s) 667
HpyAV CCTTC 3 cut(s) 242, 566, 718
HpyCH4III ACNGT 1 cut(s) 509
HpyCH4V TGCA 3 cut(s) 311, 641, 685
HpyF10VI GCNNNNNNNGC 5 cut(s) 184, 464, 545, 701, 734
HpyF3I CTNAG 3 cut(s) 188, 234, 388
Hsp92II CATG 4 cut(s) 209, 476, 559, 590
Kpn2I TCCGGA 1 cut(s) 753
Kzo9I GATC 5 cut(s) 152, 167, 171, 216, 230
LmnI GCTCC 2 cut(s) 700, 733
LpnPI CCDG 7 cut(s) 212, 227, 239, 356, 468, 631, 767
Lsp1109I GCAGC 3 cut(s) 371, 445, 625
LweI GCATC 1 cut(s) 10
MaeI CTAG 5 cut(s) 165, 272, 399, 599, 633
MalI GATC 5 cut(s) 154, 169, 173, 218, 232
MbiI CCGCTC 1 cut(s) 76
MboI GATC 5 cut(s) 152, 167, 171, 216, 230
MboII GAAGA 6 cut(s) 33, 59, 95, 100, 221, 297
MfeI CAATTG 1 cut(s) 618
MflI RGATCY 2 cut(s) 152, 230
MlsI TGGCCA 1 cut(s) 225
MluCI AATT 6 cut(s) 145, 428, 477, 490, 618, 748
MluNI TGGCCA 1 cut(s) 225
MmeI TCCRAC 1 cut(s) 393
MnlI CCTC 4 cut(s) 41, 105, 177, 510
Mox20I TGGCCA 1 cut(s) 225
MroI TCCGGA 1 cut(s) 753
MscI TGGCCA 1 cut(s) 225
MseI TTAA 4 cut(s) 488, 594, 747, 805
Msp20I TGGCCA 1 cut(s) 225
MspI CCGG 1 cut(s) 754
MspR9I CCNGG 1 cut(s) 227
MunI CAATTG 1 cut(s) 618
MvaI CCWGG 1 cut(s) 227
MwoI GCNNNNNNNGC 5 cut(s) 184, 464, 545, 701, 734
NdeII GATC 5 cut(s) 152, 167, 171, 216, 230
NlaIII CATG 4 cut(s) 209, 476, 559, 590
NlaIV GGNNCC 1 cut(s) 729
PaeR7I CTCGAG 1 cut(s) 55
PagI TCATGA 1 cut(s) 205
PfeI GAWTC 4 cut(s) 254, 437, 679, 773
PkrI GCNGC 5 cut(s) 7, 75, 386, 460, 640
Psp6I CCWGG 1 cut(s) 225
PspGI CCWGG 1 cut(s) 225
PspN4I GGNNCC 1 cut(s) 729
PsuI RGATCY 2 cut(s) 152, 230
RsaI GTAC 2 cut(s) 276, 656
RsaNI GTAC 2 cut(s) 275, 655
SaqAI TTAA 4 cut(s) 488, 594, 747, 805
SatI GCNGC 5 cut(s) 6, 74, 385, 459, 639
Sau3AI GATC 5 cut(s) 152, 167, 171, 216, 230
ScrFI CCNGG 1 cut(s) 227
SfaNI GCATC 1 cut(s) 10
Sfr274I CTCGAG 1 cut(s) 55
SlaI CTCGAG 1 cut(s) 55
SmlI CTYRAG 2 cut(s) 55, 690
SmoI CTYRAG 2 cut(s) 55, 690
Sse9I AATT 6 cut(s) 145, 428, 477, 490, 618, 748
SsiI CCGC 2 cut(s) 6, 74
SspMI CTAG 5 cut(s) 165, 272, 399, 599, 633
StyD4I CCNGG 1 cut(s) 225
StyI CCWWGG 2 cut(s) 158, 314
TaaI ACNGT 1 cut(s) 509
TaqI TCGA 4 cut(s) 56, 149, 170, 219
TaqII GACCGA 1 cut(s) 23
TasI AATT 6 cut(s) 145, 428, 477, 490, 618, 748
TauI GCSGC 2 cut(s) 8, 76
TfiI GAWTC 4 cut(s) 254, 437, 679, 773
Tru1I TTAA 4 cut(s) 488, 594, 747, 805
Tru9I TTAA 4 cut(s) 488, 594, 747, 805
TseI GCWGC 3 cut(s) 384, 458, 638
TspDTI ATGAA 3 cut(s) 222, 489, 575
TspGWI ACGGA 1 cut(s) 672
XapI RAATTY 3 cut(s) 145, 477, 490
XbaI TCTAGA 1 cut(s) 398
XhoI CTCGAG 1 cut(s) 55
XspI CTAG 5 cut(s) 165, 272, 399, 599, 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.