Rroxscaffold_3G00268740

Peptidase M50B-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001267
Physical Location & Seq
Forward (+)
61796525 .. 61799813
3289 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00268740.1

Sequence Viewer

Length: 504 bp
ATGCAAGTTCATGCAGATGAAGGTGGAGCAACACAAACCCGTGGTGGTGTATACTGGTTAATTCTGCCAGCTGGATATCTAGGTTCATCCTTTTGGGGGATGCTCTTGATTCTTGCATCTACAAATCTTCTTACAGCACGGATAGCCGCTGGATGTTTTCTAGCTGCTCTATTTATTACACTCTTTATAGCTAAAAATTGGACACTTCGAGGACTTTGTATAGGATTCATTCTGTTCCTTGGTGTAATTTGGGCTCTGCAAGAAACAACAAAAGTTCGTGTACTTCGGTATATTATTCTGTTTATAGGTGTGATGAACAGCTTATTTTCAGTATATGATATCTATGATGATCTGATATCCCGTAGAGTTCATTCTAGTGATGCTGAGAAGTTTGCAGTAGAGTGTCCTTGCTGTACTGGTTGTGGATGGGGTGTCATATGGGGCTTAATATCTTTCTTGCTTCTCTGTGGAGCCATGTACCTTGGTCTTGTGATTTTGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M50B

Protein Analysis

167

Amino Acids

18.34

Weight (kDa)

7.55

Isoelectric Point (pI)

30.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M50B PF13398 1 - 157 8.8e-36 Peptidase M50B-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 51
AciI CCGC 1 cut(s) 147
AfaI GTAC 3 cut(s) 282, 415, 479
AfiI CCNNNNNNNGG 1 cut(s) 96
AluBI AGCT 4 cut(s) 71, 164, 191, 321
AluI AGCT 4 cut(s) 71, 164, 191, 321
ApeKI GCWGC 1 cut(s) 164
BanII GRGCYC 1 cut(s) 256
BbvI GCAGC 1 cut(s) 151
BccI CCATC 1 cut(s) 420
BfaI CTAG 3 cut(s) 80, 161, 375
BisI GCNGC 2 cut(s) 147, 165
BlsI GCNGC 2 cut(s) 148, 166
BmiI GGNNCC 1 cut(s) 472
BmsI GCATC 3 cut(s) 90, 125, 370
BsaJI CCNNGG 3 cut(s) 40, 238, 481
Bsc4I CCNNNNNNNGG 1 cut(s) 96
Bse1I ACTGG 2 cut(s) 59, 421
BseDI CCNNGG 3 cut(s) 40, 238, 481
BseGI GGATG 4 cut(s) 86, 105, 158, 431
BseLI CCNNNNNNNGG 1 cut(s) 96
BseMII CTCAG 1 cut(s) 375
BseNI ACTGG 2 cut(s) 59, 421
BseXI GCAGC 1 cut(s) 151
BslI CCNNNNNNNGG 1 cut(s) 96
Bsp1286I GDGCHC 1 cut(s) 256
Bsp143I GATC 1 cut(s) 349
BspACI CCGC 1 cut(s) 147
BspCNI CTCAG 1 cut(s) 376
BspLI GGNNCC 1 cut(s) 472
BsrI ACTGG 2 cut(s) 59, 421
BssECI CCNNGG 3 cut(s) 40, 238, 481
BssMI GATC 1 cut(s) 349
BssNAI GTATAC 1 cut(s) 52
BssT1I CCWWGG 2 cut(s) 238, 481
Bst1107I GTATAC 1 cut(s) 52
BstC8I GCNNGC 1 cut(s) 69
BstDEI CTNAG 1 cut(s) 384
BstDSI CCRYGG 1 cut(s) 40
BstF5I GGATG 4 cut(s) 86, 105, 158, 431
BstKTI GATC 1 cut(s) 352
BstMBI GATC 1 cut(s) 349
BstMWI GCNNNNNNNGC 1 cut(s) 143
BstV1I GCAGC 1 cut(s) 151
BstZ17I GTATAC 1 cut(s) 52
BtgI CCRYGG 1 cut(s) 40
BtsCI GGATG 4 cut(s) 86, 105, 158, 431
Cac8I GCNNGC 1 cut(s) 69
Csp6I GTAC 3 cut(s) 281, 414, 478
CviAII CATG 2 cut(s) 11, 475
CviJI RGCY 9 cut(s) 71, 146, 164, 191, 254, 321, 444, 473, 500
CviKI_1 RGCY 9 cut(s) 71, 146, 164, 191, 254, 321, 444, 473, 500
CviQI GTAC 3 cut(s) 281, 414, 478
DdeI CTNAG 1 cut(s) 384
DpnI GATC 1 cut(s) 351
DpnII GATC 1 cut(s) 349
Eco130I CCWWGG 2 cut(s) 238, 481
Eco24I GRGCYC 1 cut(s) 256
Eco32I GATATC 3 cut(s) 77, 340, 357
EcoRV GATATC 3 cut(s) 77, 340, 357
EcoT14I CCWWGG 2 cut(s) 238, 481
EcoT38I GRGCYC 1 cut(s) 256
ErhI CCWWGG 2 cut(s) 238, 481
FaeI CATG 2 cut(s) 14, 478
FatI CATG 2 cut(s) 10, 474
FauNDI CATATG 1 cut(s) 437
FblI GTMKAC 1 cut(s) 51
Fnu4HI GCNGC 2 cut(s) 147, 165
FokI GGATG 4 cut(s) 73, 112, 165, 438
FriOI GRGCYC 1 cut(s) 256
Fsp4HI GCNGC 2 cut(s) 147, 165
FspBI CTAG 3 cut(s) 80, 161, 375
GluI GCNGC 2 cut(s) 147, 165
Hin1II CATG 2 cut(s) 14, 478
HinfI GANTC 2 cut(s) 109, 225
Hpy166II GTNNAC 2 cut(s) 52, 281
Hpy188I TCNGA 1 cut(s) 354
Hpy188III TCNNGA 1 cut(s) 106
Hpy8I GTNNAC 2 cut(s) 52, 281
HpyAV CCTTC 1 cut(s) 14
HpyCH4V TGCA 5 cut(s) 4, 14, 116, 259, 395
HpyF10VI GCNNNNNNNGC 1 cut(s) 143
HpyF3I CTNAG 1 cut(s) 384
Hsp92II CATG 2 cut(s) 14, 478
Kzo9I GATC 1 cut(s) 349
LmnI GCTCC 2 cut(s) 26, 470
LpnPI CCDG 5 cut(s) 40, 57, 81, 135, 402
Lsp1109I GCAGC 1 cut(s) 151
LweI GCATC 3 cut(s) 90, 125, 370
MaeI CTAG 3 cut(s) 80, 161, 375
MalI GATC 1 cut(s) 351
MboI GATC 1 cut(s) 349
MboII GAAGA 1 cut(s) 119
MhlI GDGCHC 1 cut(s) 256
MluCI AATT 3 cut(s) 60, 196, 246
MnlI CCTC 1 cut(s) 203
MseI TTAA 2 cut(s) 59, 446
MslI CAYNNNNRTG 2 cut(s) 15, 375
MspA1I CMGCKG 2 cut(s) 71, 149
MwoI GCNNNNNNNGC 1 cut(s) 143
NdeI CATATG 1 cut(s) 437
NdeII GATC 1 cut(s) 349
NlaIII CATG 2 cut(s) 14, 478
NlaIV GGNNCC 1 cut(s) 472
PfeI GAWTC 2 cut(s) 109, 225
PkrI GCNGC 2 cut(s) 148, 166
PspN4I GGNNCC 1 cut(s) 472
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 3 cut(s) 282, 415, 479
RsaNI GTAC 3 cut(s) 281, 414, 478
RseI CAYNNNNRTG 2 cut(s) 15, 375
SaqAI TTAA 2 cut(s) 59, 446
SatI GCNGC 2 cut(s) 147, 165
Sau3AI GATC 1 cut(s) 349
SduI GDGCHC 1 cut(s) 256
SetI ASST 8 cut(s) 25, 73, 85, 166, 193, 310, 323, 483
SfaNI GCATC 3 cut(s) 90, 125, 370
SmiMI CAYNNNNRTG 2 cut(s) 15, 375
Sse9I AATT 3 cut(s) 60, 196, 246
SsiI CCGC 1 cut(s) 147
SspMI CTAG 3 cut(s) 80, 161, 375
StyI CCWWGG 2 cut(s) 238, 481
TaqI TCGA 1 cut(s) 208
TasI AATT 3 cut(s) 60, 196, 246
TatI WGTACW 2 cut(s) 280, 413
TauI GCSGC 1 cut(s) 149
TfiI GAWTC 2 cut(s) 109, 225
Tru1I TTAA 2 cut(s) 59, 446
Tru9I TTAA 2 cut(s) 59, 446
TseI GCWGC 1 cut(s) 164
TspDTI ATGAA 5 cut(s) 33, 75, 217, 329, 359
TspGWI ACGGA 1 cut(s) 154
XmiI GTMKAC 1 cut(s) 51
XspI CTAG 3 cut(s) 80, 161, 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.