Prupe.5G222600_v2.0.a1

Peptidase M50B-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0001267
Physical Location & Seq
Reverse (-)
17285135 .. 17288759
3625 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G222600.3

Sequence Viewer

Length: 702 bp
ATGAGGCCCAATTGGGAACTCACTAACTGCTGCAACCATGAGCAAGTGGTCTTTCTCATTACCGTTTCCGTCTGCACTGTCGTTATACTTGCGCTGTGGAGGACAATACTGCTGAGACCATTTAAGCTTGTCACTGTGTTTCTTCATGAGGCGAGCCATGCTATTGCTTGTAAACTTACATGTGGTCATGTGGAAGGGATTCAAGTTCATGCAGATGAGGGTGGAACCACACAAACCCGTGGTGGTGTATACTGGTTGATTTTGCCAGCTGGATATCTTGGTTCATCCTTTTGGGGGATGCTTTTGATTCTTGCATCTACAAATCTTCTTACTGCACGAATAGCTGCTGGGTGTTTTATTGCTGCTCTACTGGTTGTACTGTTTATAGCTAAAAATTGGACACTTCGAGGACTTTGTATAGGATTCATTCTTTTCCTCGGTATAATTTGGGTTCTGCAAGAAACAACAAAAGTTCGTATACTTCGGTATATTATTCTGTTCACTGGTGTAATGAACAGCTTGTTTTCAGTCTATGATATCTATGATGATCTTATATCCCGTAGAGTTCATTCTAGTGATGCTGAGAAATTCGCAGAAGAGTGTCCTTGCTGTACTGGTTGTGGATGGGGTGTCATTTGGGGGTTTATATCTTTCTTGTTTCTCTGCGGAGCCATGTACCTCGGTCTTGTGATTTTGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M50B

Protein Analysis

234

Amino Acids

25.92

Weight (kDa)

7.51

Isoelectric Point (pI)

32.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 249, 478
AciI CCGC 1 cut(s) 666
AcsI RAATTY 1 cut(s) 587
AfaI GTAC 3 cut(s) 378, 613, 677
AfiI CCNNNNNNNGG 1 cut(s) 294
AflIII ACRYGT 1 cut(s) 179
AgsI TTSAA 1 cut(s) 203
AluBI AGCT 5 cut(s) 127, 269, 344, 389, 519
AluI AGCT 5 cut(s) 127, 269, 344, 389, 519
Alw26I GTCTC 1 cut(s) 109
AoxI GGCC 1 cut(s) 5
ApeKI GCWGC 3 cut(s) 30, 344, 362
ApoI RAATTY 1 cut(s) 587
Asp700I GAANNNNTTC 1 cut(s) 198
AspLEI GCGC 1 cut(s) 94
AspS9I GGNCC 1 cut(s) 6
BbvI GCAGC 3 cut(s) 17, 331, 349
BccI CCATC 1 cut(s) 618
BcoDI GTCTC 1 cut(s) 109
BfaI CTAG 1 cut(s) 573
BisI GCNGC 3 cut(s) 31, 345, 363
BlsI GCNGC 3 cut(s) 32, 346, 364
BmgT120I GGNCC 1 cut(s) 6
BmiI GGNNCC 2 cut(s) 226, 670
BmsI GCATC 3 cut(s) 288, 323, 568
BsaI GGTCTC 1 cut(s) 109
BsaJI CCNNGG 3 cut(s) 238, 436, 679
Bsc4I CCNNNNNNNGG 1 cut(s) 294
Bse1I ACTGG 4 cut(s) 257, 375, 508, 619
BseDI CCNNGG 3 cut(s) 238, 436, 679
BseGI GGATG 3 cut(s) 284, 303, 629
BseLI CCNNNNNNNGG 1 cut(s) 294
BseMII CTCAG 2 cut(s) 104, 573
BseNI ACTGG 4 cut(s) 257, 375, 508, 619
BseXI GCAGC 3 cut(s) 17, 331, 349
BseYI CCCAGC 1 cut(s) 347
BsgI GTGCAG 2 cut(s) 58, 318
BshFI GGCC 1 cut(s) 7
BslI CCNNNNNNNGG 1 cut(s) 294
BsmAI GTCTC 1 cut(s) 109
BsnI GGCC 1 cut(s) 7
Bso31I GGTCTC 1 cut(s) 109
Bsp143I GATC 1 cut(s) 547
BspACI CCGC 1 cut(s) 666
BspANI GGCC 1 cut(s) 7
BspCNI CTCAG 2 cut(s) 105, 574
BspHI TCATGA 1 cut(s) 145
BspLI GGNNCC 2 cut(s) 226, 670
BspTNI GGTCTC 1 cut(s) 109
BsrI ACTGG 4 cut(s) 257, 375, 508, 619
BssECI CCNNGG 3 cut(s) 238, 436, 679
BssMI GATC 1 cut(s) 547
BssNAI GTATAC 2 cut(s) 250, 479
Bst1107I GTATAC 2 cut(s) 250, 479
Bst4CI ACNGT 4 cut(s) 64, 79, 136, 381
Bst6I CTCTTC 1 cut(s) 591
BstC8I GCNNGC 2 cut(s) 154, 267
BstDEI CTNAG 2 cut(s) 113, 582
BstDSI CCRYGG 1 cut(s) 238
BstF5I GGATG 3 cut(s) 284, 303, 629
BstHHI GCGC 1 cut(s) 94
BstKTI GATC 1 cut(s) 550
BstMAI GTCTC 1 cut(s) 109
BstMBI GATC 1 cut(s) 547
BstMWI GCNNNNNNNGC 2 cut(s) 158, 341
BstNSI RCATGY 1 cut(s) 183
BstV1I GCAGC 3 cut(s) 17, 331, 349
BstZ17I GTATAC 2 cut(s) 250, 479
BsuRI GGCC 1 cut(s) 7
BtgI CCRYGG 1 cut(s) 238
BtsCI GGATG 3 cut(s) 284, 303, 629
BtsIMutI CAGTG 3 cut(s) 75, 132, 501
Cac8I GCNNGC 2 cut(s) 154, 267
CciI TCATGA 1 cut(s) 145
CfoI GCGC 1 cut(s) 94
Cfr13I GGNCC 1 cut(s) 6
Csp6I GTAC 3 cut(s) 377, 612, 676
CviAII CATG 7 cut(s) 38, 146, 158, 180, 188, 209, 673
CviJI RGCY 8 cut(s) 7, 127, 156, 269, 344, 389, 519, 671
CviKI_1 RGCY 8 cut(s) 7, 127, 156, 269, 344, 389, 519, 671
CviQI GTAC 3 cut(s) 377, 612, 676
DdeI CTNAG 2 cut(s) 113, 582
DpnI GATC 1 cut(s) 549
DpnII GATC 1 cut(s) 547
Eam1104I CTCTTC 1 cut(s) 591
EarI CTCTTC 1 cut(s) 591
Eco31I GGTCTC 1 cut(s) 109
Eco32I GATATC 2 cut(s) 275, 538
EcoRV GATATC 2 cut(s) 275, 538
FaeI CATG 7 cut(s) 41, 149, 161, 183, 191, 212, 676
FatI CATG 7 cut(s) 37, 145, 157, 179, 187, 208, 672
FblI GTMKAC 2 cut(s) 249, 478
Fnu4HI GCNGC 3 cut(s) 31, 345, 363
FokI GGATG 3 cut(s) 271, 310, 636
Fsp4HI GCNGC 3 cut(s) 31, 345, 363
FspBI CTAG 1 cut(s) 573
GlaI GCGC 1 cut(s) 93
GluI GCNGC 3 cut(s) 31, 345, 363
GsaI CCCAGC 1 cut(s) 351
HaeIII GGCC 1 cut(s) 7
HhaI GCGC 1 cut(s) 94
Hin1II CATG 7 cut(s) 41, 149, 161, 183, 191, 212, 676
Hin6I GCGC 1 cut(s) 92
HinP1I GCGC 1 cut(s) 92
HindIII AAGCTT 1 cut(s) 125
HinfI GANTC 3 cut(s) 199, 307, 423
Hpy166II GTNNAC 4 cut(s) 173, 250, 479, 501
Hpy188III TCNNGA 2 cut(s) 146, 699
Hpy8I GTNNAC 4 cut(s) 173, 250, 479, 501
HpyAV CCTTC 1 cut(s) 188
HpyCH4III ACNGT 4 cut(s) 64, 79, 136, 381
HpyCH4V TGCA 6 cut(s) 33, 75, 212, 314, 335, 457
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 341
HpyF3I CTNAG 2 cut(s) 113, 582
Hsp92II CATG 7 cut(s) 41, 149, 161, 183, 191, 212, 676
HspAI GCGC 1 cut(s) 92
Kzo9I GATC 1 cut(s) 547
LmnI GCTCC 1 cut(s) 668
LpnPI CCDG 7 cut(s) 238, 255, 279, 333, 356, 489, 600
Lsp1109I GCAGC 3 cut(s) 17, 331, 349
LweI GCATC 3 cut(s) 288, 323, 568
MaeI CTAG 1 cut(s) 573
MaeIII GTNAC 1 cut(s) 130
MalI GATC 1 cut(s) 549
MboI GATC 1 cut(s) 547
MboII GAAGA 3 cut(s) 134, 317, 608
MfeI CAATTG 1 cut(s) 10
MluCI AATT 4 cut(s) 10, 394, 444, 587
MnlI CCTC 6 cut(s) 93, 142, 211, 401, 446, 689
MroXI GAANNNNTTC 1 cut(s) 198
MseI TTAA 1 cut(s) 123
MslI CAYNNNNRTG 2 cut(s) 213, 573
MspA1I CMGCKG 1 cut(s) 269
MunI CAATTG 1 cut(s) 10
MwoI GCNNNNNNNGC 2 cut(s) 158, 341
NdeII GATC 1 cut(s) 547
NlaIII CATG 7 cut(s) 41, 149, 161, 183, 191, 212, 676
NlaIV GGNNCC 2 cut(s) 226, 670
NmuCI GTSAC 1 cut(s) 130
NspI RCATGY 1 cut(s) 183
PagI TCATGA 1 cut(s) 145
PciI ACATGT 1 cut(s) 179
PdmI GAANNNNTTC 1 cut(s) 198
PfeI GAWTC 3 cut(s) 199, 307, 423
PkrI GCNGC 3 cut(s) 32, 346, 364
PscI ACATGT 1 cut(s) 179
PspFI CCCAGC 1 cut(s) 347
PspN4I GGNNCC 2 cut(s) 226, 670
PspPI GGNCC 1 cut(s) 6
PvuII CAGCTG 1 cut(s) 269
RsaI GTAC 3 cut(s) 378, 613, 677
RsaNI GTAC 3 cut(s) 377, 612, 676
RseI CAYNNNNRTG 2 cut(s) 213, 573
SaqAI TTAA 1 cut(s) 123
SatI GCNGC 3 cut(s) 31, 345, 363
Sau3AI GATC 1 cut(s) 547
Sau96I GGNCC 1 cut(s) 6
SetI ASST 6 cut(s) 129, 271, 346, 391, 521, 681
SfaNI GCATC 3 cut(s) 288, 323, 568
SmiMI CAYNNNNRTG 2 cut(s) 213, 573
Sse9I AATT 4 cut(s) 10, 394, 444, 587
SsiI CCGC 1 cut(s) 666
SspMI CTAG 1 cut(s) 573
TaaI ACNGT 4 cut(s) 64, 79, 136, 381
TaqI TCGA 1 cut(s) 406
TaqII GACCGA 1 cut(s) 671
TasI AATT 4 cut(s) 10, 394, 444, 587
TatI WGTACW 2 cut(s) 376, 611
TfiI GAWTC 3 cut(s) 199, 307, 423
Tru1I TTAA 1 cut(s) 123
Tru9I TTAA 1 cut(s) 123
TscAI CASTG 3 cut(s) 82, 139, 508
TseFI GTSAC 1 cut(s) 130
TseI GCWGC 3 cut(s) 30, 344, 362
Tsp45I GTSAC 1 cut(s) 130
TspDTI ATGAA 6 cut(s) 134, 197, 273, 415, 527, 557
TspGWI ACGGA 1 cut(s) 58
TspRI CASTG 3 cut(s) 82, 139, 508
XapI RAATTY 1 cut(s) 587
XceI RCATGY 1 cut(s) 183
XmiI GTMKAC 2 cut(s) 249, 478
XmnI GAANNNNTTC 1 cut(s) 198
XspI CTAG 1 cut(s) 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.