AT2G03330

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0002817
Physical Location & Seq
Forward (+)
1008460 .. 1014122
5663 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G03330.1

Sequence Viewer

Length: 1158 bp
ATGAGGTTTGAGTGGTCTTCTACTAGAGCTGTCGAAGAAGGCCCGGTTCATCACATCCAACATCGATTCAAGGAACGTGTCAGAGTTATGGGTAGTTGCGACGGGCTTGTCTGCATCTTGGACCACGACATTTTGATAAGTCCAATTATTGTGACAAACCCATCTATGCGACGGTCTCAAAACCTCCCTCTTTCCATGACTCAGCTAAAGTATTTGGACAATAAGATGCCACTTCCAAAGGTGTTCCCTTTCCTAGGATTTGGTAAAGACAATGTTACAGGAACGTACAAACTAGTTTGGTTACATGATGACAAAAGTAACAACATCTCATCATGCGAGGTTTTCGACTTTGAGGTCAAGAAATGGAGATCTCTCCGTTCGCTTCCTCCTTCCTCTCAGCGTCTTCATCACACTTTACCCTTACGTCTTCCTTCTCATCACCGATCAAACCTCGCTACTCACAACAACGATGATGACATCGATACCAAAACAAAGGTCTTAATTCTAATCGGTTTCATCGTTGTTATCACCGTCTTCTATCTCTTAGCTATTGGATCAATCGCTTATAGGGGTTTTCTGATTCCAGGGATTTTAGCTTTCTTGTTGGTTAAGCTTATTGAGATCATTCCCGGTGTAGAATTCGATTACGCCTCTTCGTATTTCCAAGGTTTCGTAACCCTAGTAACGATTATCTCGATTGCGGTTGCGGTTAAGCTCTATGTGAATTGGATTCTTGCTTGGGTTGTTGTTGTTGTTGAATCGGCTTGGGGTATAACACCGTTGAAGACAAGTAAACGCTTGGTTAAAGGAATGAAATGTGCTTCTTTGTCGATTATCTTCTTCTTCGCTTCAACGGAATCGATTCTCGTTTGGATTAGTACCTTAGCCGCCTATGCTCTGCTCAACGATAATGAGAATGGTGGGAAGTCGTGGAAAGGTTCTTTCTTTTTTGTACAGAATTGGATCACGTCCGCGTTGCTAACGCTATTGATGTTGTATAATCTTGTGGCGACTACTGTGATGTATATGTATTGTAAAGCTGTTCATGGTGAGCTTGTTGGGGAGATAGCTGAAGAGTTTGCTAGAGAATATGTTAGCTTGCCTTTTGATGATGGTAAAGTTCCTCACTTGGTCTCTGTAGCTTACAACAACATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

385

Amino Acids

43.52

Weight (kDa)

8.66

Isoelectric Point (pI)

41.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_3 PF08268 18 - 126 2.3e-08 F-box associated beta propeller domain
FBA_1 PF07734 24 - 129 1.1e-08 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 107, 353
AccII CGCG 1 cut(s) 974
AciI CCGC 4 cut(s) 701, 707, 888, 972
AclWI GGATC 2 cut(s) 562, 971
AcsI RAATTY 1 cut(s) 638
AcuI CTGAAG 1 cut(s) 1092
AfaI GTAC 3 cut(s) 287, 880, 954
AfiI CCNNNNNNNGG 1 cut(s) 254
AflIII ACRYGT 1 cut(s) 76
AgsI TTSAA 4 cut(s) 70, 758, 784, 852
AhlI ACTAGT 1 cut(s) 292
AjiI CACGTC 1 cut(s) 969
AjnI CCWGG 1 cut(s) 583
Alw26I GTCTC 2 cut(s) 180, 1138
AlwI GGATC 2 cut(s) 562, 971
AoxI GGCC 1 cut(s) 40
ApoI RAATTY 1 cut(s) 638
ArsI GACNNNNNNTTYG 2 cut(s) 480, 512
Asp700I GAANNNNTTC 1 cut(s) 861
AspA2I CCTAGG 1 cut(s) 253
AspS9I GGNCC 2 cut(s) 41, 121
AsuC2I CCSGG 2 cut(s) 44, 630
AsuHPI GGTGA 3 cut(s) 431, 520, 1061
AvaII GGWCC 1 cut(s) 121
AvrII CCTAGG 1 cut(s) 253
BbsI GAAGAC 5 cut(s) 9, 395, 419, 526, 791
BccI CCATC 2 cut(s) 169, 1106
BciT130I CCWGG 1 cut(s) 585
BcnI CCSGG 2 cut(s) 44, 630
BcoDI GTCTC 2 cut(s) 180, 1138
BcuI ACTAGT 1 cut(s) 292
BfaI CTAG 5 cut(s) 24, 254, 293, 680, 1083
BfmI CTRYAG 1 cut(s) 1137
BglII AGATCT 1 cut(s) 368
BisI GCNGC 1 cut(s) 888
BlnI CCTAGG 1 cut(s) 253
BlsI GCNGC 1 cut(s) 889
Bme1390I CCNGG 3 cut(s) 44, 585, 630
Bme18I GGWCC 1 cut(s) 121
BmgBI CACGTC 1 cut(s) 969
BmgT120I GGNCC 2 cut(s) 41, 121
BmrFI CCNGG 3 cut(s) 44, 585, 630
BmsI GCATC 2 cut(s) 123, 216
BpiI GAAGAC 5 cut(s) 9, 395, 419, 526, 791
Bpu10I CCTNAGC 1 cut(s) 883
BpuMI CCSGG 2 cut(s) 44, 630
Bsa29I ATCGAT 3 cut(s) 64, 480, 860
BsaI GGTCTC 2 cut(s) 180, 1138
BsaJI CCNNGG 3 cut(s) 253, 584, 664
Bsc4I CCNNNNNNNGG 1 cut(s) 254
BseBI CCWGG 1 cut(s) 585
BseCI ATCGAT 3 cut(s) 64, 480, 860
BseDI CCNNGG 3 cut(s) 253, 584, 664
BseGI GGATG 1 cut(s) 54
BseLI CCNNNNNNNGG 1 cut(s) 254
BseMII CTCAG 2 cut(s) 215, 410
Bsh1236I CGCG 1 cut(s) 974
BshFI GGCC 1 cut(s) 42
BshVI ATCGAT 3 cut(s) 64, 480, 860
BsiSI CCGG 2 cut(s) 44, 630
BslI CCNNNNNNNGG 1 cut(s) 254
BsmAI GTCTC 2 cut(s) 180, 1138
BsnI GGCC 1 cut(s) 42
Bso31I GGTCTC 2 cut(s) 180, 1138
Bsp1407I TGTACA 1 cut(s) 952
Bsp143I GATC 5 cut(s) 368, 443, 554, 621, 963
BspACI CCGC 4 cut(s) 701, 707, 888, 972
BspANI GGCC 1 cut(s) 42
BspCNI CTCAG 2 cut(s) 214, 409
BspDI ATCGAT 3 cut(s) 64, 480, 860
BspFNI CGCG 1 cut(s) 974
BspPI GGATC 2 cut(s) 562, 971
BspTNI GGTCTC 2 cut(s) 180, 1138
BsrGI TGTACA 1 cut(s) 952
BssECI CCNNGG 3 cut(s) 253, 584, 664
BssMI GATC 5 cut(s) 368, 443, 554, 621, 963
BssT1I CCWWGG 2 cut(s) 253, 664
Bst2UI CCWGG 1 cut(s) 585
Bst4CI ACNGT 4 cut(s) 174, 532, 780, 1018
Bst6I CTCTTC 2 cut(s) 658, 1068
BstAUI TGTACA 1 cut(s) 952
BstC8I GCNNGC 1 cut(s) 1100
BstDEI CTNAG 4 cut(s) 201, 396, 544, 883
BstENI CCTNNNNNAGG 1 cut(s) 252
BstF5I GGATG 1 cut(s) 54
BstFNI CGCG 1 cut(s) 974
BstKTI GATC 5 cut(s) 371, 446, 557, 624, 966
BstMAI GTCTC 2 cut(s) 180, 1138
BstMBI GATC 5 cut(s) 368, 443, 554, 621, 963
BstMWI GCNNNNNNNGC 1 cut(s) 893
BstNI CCWGG 1 cut(s) 585
BstSCI CCNGG 3 cut(s) 42, 583, 628
BstSFI CTRYAG 1 cut(s) 1137
BstUI CGCG 1 cut(s) 974
BstV2I GAAGAC 5 cut(s) 9, 395, 419, 526, 791
BstX2I RGATCY 1 cut(s) 368
BstYI RGATCY 1 cut(s) 368
Bsu15I ATCGAT 3 cut(s) 64, 480, 860
BsuRI GGCC 1 cut(s) 42
BsuTUI ATCGAT 3 cut(s) 64, 480, 860
BtrI CACGTC 1 cut(s) 969
BtsCI GGATG 1 cut(s) 54
Cac8I GCNNGC 1 cut(s) 1100
Cfr13I GGNCC 2 cut(s) 41, 121
ClaI ATCGAT 3 cut(s) 64, 480, 860
CseI GACGC 1 cut(s) 389
Csp6I GTAC 3 cut(s) 286, 879, 953
CviAII CATG 4 cut(s) 196, 305, 333, 1046
CviQI GTAC 3 cut(s) 286, 879, 953
DdeI CTNAG 4 cut(s) 201, 396, 544, 883
DpnI GATC 5 cut(s) 370, 445, 556, 623, 965
DpnII GATC 5 cut(s) 368, 443, 554, 621, 963
DrdI GACNNNNNNGTC 2 cut(s) 107, 353
DseDI GACNNNNNNGTC 2 cut(s) 107, 353
Eam1104I CTCTTC 2 cut(s) 658, 1068
EarI CTCTTC 2 cut(s) 658, 1068
Eco130I CCWWGG 2 cut(s) 253, 664
Eco31I GGTCTC 2 cut(s) 180, 1138
Eco47I GGWCC 1 cut(s) 121
Eco57I CTGAAG 1 cut(s) 1092
EcoNI CCTNNNNNAGG 1 cut(s) 252
EcoRI GAATTC 1 cut(s) 638
EcoRII CCWGG 1 cut(s) 583
EcoT14I CCWWGG 2 cut(s) 253, 664
ErhI CCWWGG 2 cut(s) 253, 664
FaeI CATG 4 cut(s) 199, 308, 336, 1049
FatI CATG 4 cut(s) 195, 304, 332, 1045
Fnu4HI GCNGC 1 cut(s) 888
FokI GGATG 1 cut(s) 41
Fsp4HI GCNGC 1 cut(s) 888
FspBI CTAG 5 cut(s) 24, 254, 293, 680, 1083
GluI GCNGC 1 cut(s) 888
HaeIII GGCC 1 cut(s) 42
HapII CCGG 2 cut(s) 44, 630
HgaI GACGC 1 cut(s) 389
Hin1II CATG 4 cut(s) 199, 308, 336, 1049
HindIII AAGCTT 1 cut(s) 611
HinfI GANTC 7 cut(s) 66, 199, 580, 730, 758, 857, 862
HpaII CCGG 2 cut(s) 44, 630
HphI GGTGA 3 cut(s) 431, 520, 1061
Hpy166II GTNNAC 1 cut(s) 794
Hpy188I TCNGA 3 cut(s) 83, 579, 1157
Hpy188III TCNNGA 2 cut(s) 358, 694
Hpy8I GTNNAC 1 cut(s) 794
Hpy99I CGWCG 2 cut(s) 104, 174
HpyAV CCTTC 3 cut(s) 32, 399, 441
HpyCH4III ACNGT 4 cut(s) 174, 532, 780, 1018
HpyCH4IV ACGT 4 cut(s) 76, 284, 424, 968
HpyCH4V TGCA 1 cut(s) 114
HpyF10VI GCNNNNNNNGC 1 cut(s) 893
HpyF3I CTNAG 4 cut(s) 201, 396, 544, 883
HpySE526I ACGT 4 cut(s) 76, 284, 424, 968
Hsp92II CATG 4 cut(s) 199, 308, 336, 1049
Kzo9I GATC 5 cut(s) 368, 443, 554, 621, 963
LpnPI CCDG 5 cut(s) 57, 264, 570, 597, 643
LweI GCATC 2 cut(s) 123, 216
MaeI CTAG 5 cut(s) 24, 254, 293, 680, 1083
MaeII ACGT 4 cut(s) 76, 284, 424, 968
MaeIII GTNAC 6 cut(s) 151, 274, 300, 317, 673, 682
MalI GATC 5 cut(s) 370, 445, 556, 623, 965
MboI GATC 5 cut(s) 368, 443, 554, 621, 963
MflI RGATCY 1 cut(s) 368
MluCI AATT 5 cut(s) 144, 501, 638, 724, 958
MlyI GAGTC 1 cut(s) 193
MmeI TCCRAC 1 cut(s) 82
MnlI CCTC 9 cut(s) 194, 198, 331, 346, 396, 403, 461, 661, 1134
MroXI GAANNNNTTC 1 cut(s) 861
MseI TTAA 4 cut(s) 500, 609, 711, 804
MspI CCGG 2 cut(s) 44, 630
MspR9I CCNGG 3 cut(s) 44, 585, 630
MvaI CCWGG 1 cut(s) 585
MvnI CGCG 1 cut(s) 974
MwoI GCNNNNNNNGC 1 cut(s) 893
NciI CCSGG 2 cut(s) 44, 630
NdeII GATC 5 cut(s) 368, 443, 554, 621, 963
NlaIII CATG 4 cut(s) 199, 308, 336, 1049
NmuCI GTSAC 1 cut(s) 151
PcsI WCGNNNNNNNCGW 2 cut(s) 516, 692
PdmI GAANNNNTTC 1 cut(s) 861
PfeI GAWTC 6 cut(s) 66, 580, 730, 758, 857, 862
PkrI GCNGC 1 cut(s) 889
PleI GAGTC 1 cut(s) 193
PpsI GAGTC 1 cut(s) 193
Psp6I CCWGG 1 cut(s) 583
PspGI CCWGG 1 cut(s) 583
PspPI GGNCC 2 cut(s) 41, 121
PsuI RGATCY 1 cut(s) 368
RsaI GTAC 3 cut(s) 287, 880, 954
RsaNI GTAC 3 cut(s) 286, 879, 953
SaqAI TTAA 4 cut(s) 500, 609, 711, 804
SatI GCNGC 1 cut(s) 888
Sau3AI GATC 5 cut(s) 368, 443, 554, 621, 963
Sau96I GGNCC 2 cut(s) 41, 121
SchI GAGTC 1 cut(s) 193
ScrFI CCNGG 3 cut(s) 44, 585, 630
SfaNI GCATC 2 cut(s) 123, 216
SfcI CTRYAG 1 cut(s) 1137
SinI GGWCC 1 cut(s) 121
SpeI ACTAGT 1 cut(s) 292
Sse9I AATT 5 cut(s) 144, 501, 638, 724, 958
SsiI CCGC 4 cut(s) 701, 707, 888, 972
SspMI CTAG 5 cut(s) 24, 254, 293, 680, 1083
StyD4I CCNGG 3 cut(s) 42, 583, 628
StyI CCWWGG 2 cut(s) 253, 664
TaaI ACNGT 4 cut(s) 174, 532, 780, 1018
TaiI ACGT 4 cut(s) 79, 287, 427, 971
TaqI TCGA 8 cut(s) 33, 64, 345, 480, 642, 695, 830, 860
TasI AATT 5 cut(s) 144, 501, 638, 724, 958
TatI WGTACW 1 cut(s) 952
TauI GCSGC 1 cut(s) 890
TfiI GAWTC 6 cut(s) 66, 580, 730, 758, 857, 862
Tru1I TTAA 4 cut(s) 500, 609, 711, 804
Tru9I TTAA 4 cut(s) 500, 609, 711, 804
TseFI GTSAC 1 cut(s) 151
Tsp45I GTSAC 1 cut(s) 151
TspDTI ATGAA 5 cut(s) 38, 395, 505, 827, 1034
TspGWI ACGGA 2 cut(s) 365, 869
VpaK11BI GGWCC 1 cut(s) 121
XagI CCTNNNNNAGG 1 cut(s) 252
XapI RAATTY 1 cut(s) 638
XmaJI CCTAGG 1 cut(s) 253
XmnI GAANNNNTTC 1 cut(s) 861
XspI CTAG 5 cut(s) 24, 254, 293, 680, 1083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.