pycom13g05970

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0002817
Physical Location & Seq
Reverse (-)
3926435 .. 3927442
1008 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g05970.1

Sequence Viewer

Length: 1008 bp
ATGGCAACCCCATCTCCGCCTCCAACCGTTTGCACAGTCCTCAACGAATCCAAGCGCATAGTCAACGCCCACTCCTGCCACTTCCTAGCCCTCTCAGTCCTCTTCCTCCTCCCCCTCGCCTTCTCCTCCACCGTTTACCCCACAATCCAGAACCTCCTCGCCGATCGAGTCCCAGACAACTCCAAGATATTCCTCGAAATCTCAACTTTCGACCCCCATCAGCACCAACCCATCATCCCCGCCAAAACCCTTCTCATCGCCCTTGCTTTCTCCCTCTTCGCCGTCGTCTTCTCTCTCTGCGCCCTCGGGTCGATCACTTACAGTGTGTTTCACGGATTCTATGGCCGGCCGGTCAAGCTCGTCTCCGCCGTCAAATCCGTCGTCTCCTCCTTCTTCCCTCTATTGGGCACTGCTTTCATTTCCCAGGTCCTCGTTTTATTGGTTTTTGTTCTTTTCGGGCTGTACGTGTTCTTGGTGGTTCGAGGAGCTGAGCTCATGGGGTTTGAATTGGAGTACTCTTCGCCTCATTTCATTGGGTTTTTAGCGGTTGTTGTGACTGTTCTGTTTCTGGTTTTGCTGCATCTTCAAGTGAATTGGACACTGGCTGGTGTTGTTGTTGTGGTGGAATCAAAATGGGGATTCGGGTCCTTGACCCGAAGTGCGAATTTGATCAAGGGAATGAGATGGGTGGCATTGTGGTTGCTGATATTTTTCGGGTTTTTCGTGGGGGTTTTGGGGTTCTGCAGTTGGGTTTCGGCATTGGATTTGAAGGGAGATACTGATGGGTGGAAGAGCTGGGCGTTTCTGGTGCAAATTGTGGTGACTGCGACTTCTCTCGTGCTGCTGATGCTCTACAATGCCGCCGCAAATACGGTGTTGTATATGTATTGCAAGGCCGTGAATGGTGAGCTTGCCATGGAAATTGCTGAGGAGTTTGCTAGGGAGTATGTGAGCTTGCCCTTCGACAACGGAAAAGTCCCTCATCTTGTTTCGGTTGCATATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

336

Amino Acids

36.83

Weight (kDa)

7.08

Isoelectric Point (pI)

29.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 17, 240, 366, 545, 861, 864
AcoI YGGCCR 2 cut(s) 343, 347
AcsI RAATTY 1 cut(s) 664
AfaI GTAC 2 cut(s) 464, 515
AfiI CCNNNNNNNGG 2 cut(s) 403, 404
AflIII ACRYGT 1 cut(s) 465
AgsI TTSAA 3 cut(s) 506, 587, 769
AjnI CCWGG 1 cut(s) 423
AluBI AGCT 6 cut(s) 358, 488, 493, 795, 910, 954
AluI AGCT 6 cut(s) 358, 488, 493, 795, 910, 954
Alw21I GWGCWC 1 cut(s) 495
Alw26I GTCTC 2 cut(s) 367, 388
Ama87I CYCGRG 1 cut(s) 305
AoxI GGCC 3 cut(s) 343, 347, 894
ApeKI GCWGC 2 cut(s) 577, 841
ApoI RAATTY 1 cut(s) 664
ArsI GACNNNNNNTTYG 2 cut(s) 366, 398
AspLEI GCGC 2 cut(s) 57, 302
AspS9I GGNCC 2 cut(s) 427, 645
AsuHPI GGTGA 2 cut(s) 832, 917
AvaI CYCGRG 1 cut(s) 305
AvaII GGWCC 2 cut(s) 427, 645
BaeGI GKGCMC 1 cut(s) 410
BanII GRGCYC 1 cut(s) 495
BauI CACGAG 1 cut(s) 836
BbsI GAAGAC 1 cut(s) 280
Bbv12I GWGCWC 1 cut(s) 495
BbvCI CCTCAGC 1 cut(s) 927
BbvI GCAGC 2 cut(s) 564, 828
BccI CCATC 5 cut(s) 19, 225, 239, 678, 776
BceAI ACGGC 3 cut(s) 266, 353, 881
BciT130I CCWGG 1 cut(s) 425
BclI TGATCA 1 cut(s) 669
BcoDI GTCTC 2 cut(s) 367, 388
BfaI CTAG 2 cut(s) 86, 939
BfmI CTRYAG 1 cut(s) 742
BisI GCNGC 4 cut(s) 578, 842, 861, 864
BlpI GCTNAGC 1 cut(s) 489
BlsI GCNGC 4 cut(s) 579, 843, 862, 865
BmcAI AGTACT 1 cut(s) 515
Bme1390I CCNGG 1 cut(s) 425
Bme18I GGWCC 2 cut(s) 427, 645
BmeT110I CYCGRG 1 cut(s) 305
BmgT120I GGNCC 2 cut(s) 427, 645
BmiI GGNNCC 1 cut(s) 646
BmrFI CCNGG 1 cut(s) 425
BmsI GCATC 2 cut(s) 589, 837
BpiI GAAGAC 1 cut(s) 280
BplI GAGNNNNNCTC 2 cut(s) 477, 509
Bpu10I CCTNAGC 1 cut(s) 927
Bpu1102I GCTNAGC 1 cut(s) 489
BsaAI YACGTR 1 cut(s) 466
BsaJI CCNNGG 3 cut(s) 304, 423, 915
BsaXI ACNNNNNCTCC 3 cut(s) 28, 56, 86
Bsc4I CCNNNNNNNGG 2 cut(s) 403, 404
Bse118I RCCGGY 2 cut(s) 345, 349
Bse1I ACTGG 1 cut(s) 606
BseBI CCWGG 1 cut(s) 425
BseDI CCNNGG 3 cut(s) 304, 423, 915
BseGI GGATG 1 cut(s) 234
BseLI CCNNNNNNNGG 2 cut(s) 403, 404
BseMII CTCAG 3 cut(s) 108, 480, 918
BseNI ACTGG 1 cut(s) 606
BseRI GAGGAG 6 cut(s) 98, 115, 146, 376, 498, 944
BseSI GKGCMC 1 cut(s) 410
BseX3I CGGCCG 1 cut(s) 347
BseXI GCAGC 2 cut(s) 564, 828
BseYI CCCAGC 1 cut(s) 795
Bsh1285I CGRYCG 2 cut(s) 166, 350
BshFI GGCC 3 cut(s) 345, 349, 896
BsiEI CGRYCG 2 cut(s) 166, 350
BsiHKAI GWGCWC 1 cut(s) 495
BsiHKCI CYCGRG 1 cut(s) 305
BsiSI CCGG 2 cut(s) 346, 350
BslFI GGGAC 2 cut(s) 155, 962
BslI CCNNNNNNNGG 2 cut(s) 403, 404
BsmAI GTCTC 2 cut(s) 367, 388
BsmBI CGTCTC 2 cut(s) 367, 388
BsmFI GGGAC 2 cut(s) 155, 962
BsnI GGCC 3 cut(s) 345, 349, 896
BsoBI CYCGRG 1 cut(s) 305
Bsp1286I GDGCHC 2 cut(s) 410, 495
Bsp143I GATC 3 cut(s) 163, 312, 669
Bsp1720I GCTNAGC 1 cut(s) 489
Bsp19I CCATGG 1 cut(s) 915
BspACI CCGC 6 cut(s) 17, 240, 366, 545, 861, 864
BspANI GGCC 3 cut(s) 345, 349, 896
BspCNI CTCAG 3 cut(s) 107, 481, 919
BspLI GGNNCC 1 cut(s) 646
BspMAI CTGCAG 1 cut(s) 746
BspQI GCTCTTC 1 cut(s) 785
BsrFI RCCGGY 2 cut(s) 345, 349
BsrI ACTGG 1 cut(s) 606
BssAI RCCGGY 2 cut(s) 345, 349
BssECI CCNNGG 3 cut(s) 304, 423, 915
BssMI GATC 3 cut(s) 163, 312, 669
BssSI CACGAG 1 cut(s) 836
BssT1I CCWWGG 1 cut(s) 915
Bst2BI CACGAG 1 cut(s) 836
Bst2UI CCWGG 1 cut(s) 425
Bst4CI ACNGT 6 cut(s) 28, 37, 133, 323, 559, 874
Bst6I CTCTTC 4 cut(s) 107, 281, 523, 785
BstBAI YACGTR 1 cut(s) 466
BstC8I GCNNGC 3 cut(s) 347, 912, 956
BstDEI CTNAG 3 cut(s) 94, 489, 927
BstDSI CCRYGG 1 cut(s) 915
BstF5I GGATG 1 cut(s) 234
BstHHI GCGC 2 cut(s) 57, 302
BstKTI GATC 3 cut(s) 166, 315, 672
BstMAI GTCTC 2 cut(s) 367, 388
BstMBI GATC 3 cut(s) 163, 312, 669
BstMCI CGRYCG 2 cut(s) 166, 350
BstMWI GCNNNNNNNGC 2 cut(s) 355, 847
BstNI CCWGG 1 cut(s) 425
BstSCI CCNGG 1 cut(s) 423
BstSFI CTRYAG 1 cut(s) 742
BstSLI GKGCMC 1 cut(s) 410
BstV1I GCAGC 2 cut(s) 564, 828
BstV2I GAAGAC 1 cut(s) 280
BstZI CGGCCG 1 cut(s) 347
BsuRI GGCC 3 cut(s) 345, 349, 896
BtgI CCRYGG 1 cut(s) 915
BtgZI GCGATG 1 cut(s) 241
BtsCI GGATG 1 cut(s) 234
BtsI GCAGTG 1 cut(s) 408
BtsIMutI CAGTG 3 cut(s) 328, 408, 599
Cac8I GCNNGC 3 cut(s) 347, 912, 956
CfoI GCGC 2 cut(s) 57, 302
Cfr10I RCCGGY 2 cut(s) 345, 349
Cfr13I GGNCC 2 cut(s) 427, 645
Csp6I GTAC 2 cut(s) 463, 514
CviAII CATG 2 cut(s) 496, 916
CviQI GTAC 2 cut(s) 463, 514
DdeI CTNAG 3 cut(s) 94, 489, 927
DpnI GATC 3 cut(s) 165, 314, 671
DpnII GATC 3 cut(s) 163, 312, 669
EaeI YGGCCR 2 cut(s) 343, 347
EagI CGGCCG 1 cut(s) 347
Eam1104I CTCTTC 4 cut(s) 107, 281, 523, 785
EarI CTCTTC 4 cut(s) 107, 281, 523, 785
EciI GGCGGA 2 cut(s) 6, 355
Ecl136II GAGCTC 1 cut(s) 493
EclXI CGGCCG 1 cut(s) 347
Eco130I CCWWGG 1 cut(s) 915
Eco24I GRGCYC 1 cut(s) 495
Eco47I GGWCC 2 cut(s) 427, 645
Eco52I CGGCCG 1 cut(s) 347
Eco53kI GAGCTC 1 cut(s) 493
Eco88I CYCGRG 1 cut(s) 305
EcoICRI GAGCTC 1 cut(s) 493
EcoO109I RGGNCCY 2 cut(s) 427, 645
EcoRII CCWGG 1 cut(s) 423
EcoT14I CCWWGG 1 cut(s) 915
EcoT38I GRGCYC 1 cut(s) 495
ErhI CCWWGG 1 cut(s) 915
Esp3I CGTCTC 2 cut(s) 367, 388
FaeI CATG 2 cut(s) 499, 919
FaiI YATR 9 cut(s) 59, 342, 497, 882, 884, 917, 948, 1000, 1002
FaqI GGGAC 2 cut(s) 155, 962
FatI CATG 2 cut(s) 495, 915
FauI CCCGC 1 cut(s) 247
FauNDI CATATG 1 cut(s) 1000
FbaI TGATCA 1 cut(s) 669
Fnu4HI GCNGC 4 cut(s) 578, 842, 861, 864
FokI GGATG 1 cut(s) 221
FriOI GRGCYC 1 cut(s) 495
FseI GGCCGGCC 1 cut(s) 349
Fsp4HI GCNGC 4 cut(s) 578, 842, 861, 864
FspBI CTAG 2 cut(s) 86, 939
GlaI GCGC 2 cut(s) 56, 301
GluI GCNGC 4 cut(s) 578, 842, 861, 864
GsaI CCCAGC 1 cut(s) 799
HaeIII GGCC 3 cut(s) 345, 349, 896
HapII CCGG 2 cut(s) 346, 350
HhaI GCGC 2 cut(s) 57, 302
Hin1II CATG 2 cut(s) 499, 919
Hin6I GCGC 2 cut(s) 55, 300
HinP1I GCGC 2 cut(s) 55, 300
HincII GTYRAC 1 cut(s) 64
HindII GTYRAC 1 cut(s) 64
HinfI GANTC 5 cut(s) 47, 168, 336, 626, 639
HpaII CCGG 2 cut(s) 346, 350
HphI GGTGA 2 cut(s) 832, 917
Hpy166II GTNNAC 2 cut(s) 64, 136
Hpy188III TCNNGA 1 cut(s) 148
Hpy8I GTNNAC 2 cut(s) 64, 136
Hpy99I CGWCG 2 cut(s) 287, 383
HpyAV CCTTC 5 cut(s) 130, 260, 400, 763, 970
HpyCH4III ACNGT 6 cut(s) 28, 37, 133, 323, 559, 874
HpyCH4IV ACGT 1 cut(s) 465
HpyCH4V TGCA 6 cut(s) 33, 580, 744, 811, 891, 998
HpyF10VI GCNNNNNNNGC 2 cut(s) 355, 847
HpyF3I CTNAG 3 cut(s) 94, 489, 927
HpySE526I ACGT 1 cut(s) 465
Hsp92II CATG 2 cut(s) 499, 919
HspAI GCGC 2 cut(s) 55, 300
KroI GCCGGC 1 cut(s) 345
KroNI GCCGGC 1 cut(s) 347
Ksp22I TGATCA 1 cut(s) 669
Kzo9I GATC 3 cut(s) 163, 312, 669
LguI GCTCTTC 1 cut(s) 785
LmnI GCTCC 1 cut(s) 485
Lsp1109I GCAGC 2 cut(s) 564, 828
LweI GCATC 2 cut(s) 589, 837
MaeI CTAG 2 cut(s) 86, 939
MaeII ACGT 1 cut(s) 465
MaeIII GTNAC 2 cut(s) 553, 820
MalI GATC 3 cut(s) 165, 314, 671
MboI GATC 3 cut(s) 163, 312, 669
MboII GAAGA 7 cut(s) 94, 268, 280, 385, 510, 575, 802
MhlI GDGCHC 2 cut(s) 410, 495
MluCI AATT 5 cut(s) 506, 592, 664, 813, 921
MlyI GAGTC 1 cut(s) 177
MmeI TCCRAC 1 cut(s) 47
MroNI GCCGGC 1 cut(s) 345
MspI CCGG 2 cut(s) 346, 350
MspR9I CCNGG 1 cut(s) 425
MvaI CCWGG 1 cut(s) 425
MwoI GCNNNNNNNGC 2 cut(s) 355, 847
NaeI GCCGGC 1 cut(s) 347
NcoI CCATGG 1 cut(s) 915
NdeI CATATG 1 cut(s) 1000
NdeII GATC 3 cut(s) 163, 312, 669
NgoMIV GCCGGC 1 cut(s) 345
NlaIII CATG 2 cut(s) 499, 919
NlaIV GGNNCC 1 cut(s) 646
NmuCI GTSAC 2 cut(s) 553, 820
PciSI GCTCTTC 1 cut(s) 785
PcsI WCGNNNNNNNCGW 3 cut(s) 366, 462, 720
PdiI GCCGGC 1 cut(s) 347
PfeI GAWTC 4 cut(s) 47, 336, 626, 639
PkrI GCNGC 4 cut(s) 579, 843, 862, 865
Ple19I CGATCG 1 cut(s) 166
PleI GAGTC 1 cut(s) 176
PpsI GAGTC 1 cut(s) 176
Ppu21I YACGTR 1 cut(s) 466
PpuMI RGGWCCY 2 cut(s) 427, 645
Psp124BI GAGCTC 1 cut(s) 495
Psp5II RGGWCCY 2 cut(s) 427, 645
Psp6I CCWGG 1 cut(s) 423
PspFI CCCAGC 1 cut(s) 795
PspGI CCWGG 1 cut(s) 423
PspN4I GGNNCC 1 cut(s) 646
PspPI GGNCC 2 cut(s) 427, 645
PspPPI RGGWCCY 2 cut(s) 427, 645
PstI CTGCAG 1 cut(s) 746
PvuI CGATCG 1 cut(s) 166
RigI GGCCGGCC 1 cut(s) 349
RsaI GTAC 2 cut(s) 464, 515
RsaNI GTAC 2 cut(s) 463, 514
SacI GAGCTC 1 cut(s) 495
SapI GCTCTTC 1 cut(s) 785
SatI GCNGC 4 cut(s) 578, 842, 861, 864
Sau3AI GATC 3 cut(s) 163, 312, 669
Sau96I GGNCC 2 cut(s) 427, 645
ScaI AGTACT 1 cut(s) 515
SchI GAGTC 1 cut(s) 177
ScrFI CCNGG 1 cut(s) 425
SduI GDGCHC 2 cut(s) 410, 495
SetI ASST 9 cut(s) 156, 360, 429, 468, 490, 495, 797, 912, 956
SfaNI GCATC 2 cut(s) 589, 837
SfcI CTRYAG 1 cut(s) 742
SinI GGWCC 2 cut(s) 427, 645
Sse9I AATT 5 cut(s) 506, 592, 664, 813, 921
SsiI CCGC 6 cut(s) 17, 240, 366, 545, 861, 864
SspMI CTAG 2 cut(s) 86, 939
SstI GAGCTC 1 cut(s) 495
StyD4I CCNGG 1 cut(s) 423
StyI CCWWGG 1 cut(s) 915
TaaI ACNGT 6 cut(s) 28, 37, 133, 323, 559, 874
TaiI ACGT 1 cut(s) 468
TaqI TCGA 6 cut(s) 166, 195, 210, 311, 481, 963
TasI AATT 5 cut(s) 506, 592, 664, 813, 921
TatI WGTACW 1 cut(s) 513
TauI GCSGC 2 cut(s) 863, 866
TfiI GAWTC 4 cut(s) 47, 336, 626, 639
TscAI CASTG 3 cut(s) 328, 415, 606
TseFI GTSAC 2 cut(s) 553, 820
TseI GCWGC 2 cut(s) 577, 841
Tsp45I GTSAC 2 cut(s) 553, 820
TspDTI ATGAA 2 cut(s) 406, 520
TspGWI ACGGA 3 cut(s) 348, 367, 984
TspRI CASTG 3 cut(s) 328, 415, 606
VpaK11BI GGWCC 2 cut(s) 427, 645
XapI RAATTY 1 cut(s) 664
XspI CTAG 2 cut(s) 86, 939
ZrmI AGTACT 1 cut(s) 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.