AT2G10970

Invertase pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0005336
Physical Location & Seq
Reverse (-)
4332795 .. 4333638
844 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G10970.1

Sequence Viewer

Length: 585 bp
ATGGTTTGTTTATATGCTTCTCATGCATACCTATCAATCCATATCCTAACCTTCCTTCTCCTCTCGCGGTGGCCATATACCAATGCAAAATCCAACATTGCTACCCATTTATCAACCAAACCAAAGACATGTCCATATCCATCATTACTGATATCAAAGGCTTGTAAAGGCACCGCGTCATTCTCGTCCCTAGAAGAAGAATGTATCAAATCCCTAACGTTAGATCATCGCACGAAATCCGCATCGACTGTATTAGAGTTGGCTAAGGCGGCTCTTTCTCTAGCCATGGAGAAGGCAGAGCACACACAGTTTCTCATTGGTTCTCCTAAGAAGCCATGTTTCAAGAGTTGTATGGAGAACTATAAAGATTCTGTTGTCGAGGGGCTAATGAAGGCACAGCGGTCAATGGGCAATGGTGATGTTGATGAGACTGATGACGACCTTAGTCTCGCACGTGATGCTGCTGACTATTGCAATATGATTTTGTCTGTTGATCCAGATGATACAAGATCCTTAGTTTTTGCGGCCAACGTGGATGTGTATAATCACATTACGTTTGTGATGAGTGTAGCAGACTTGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

194

Amino Acids

21.35

Weight (kDa)

5.84

Isoelectric Point (pI)

39.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 50 - 162 1.4e-10 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 170
AccII CGCG 2 cut(s) 67, 176
AciI CCGC 6 cut(s) 67, 174, 240, 269, 400, 524
AclI AACGTT 1 cut(s) 218
AclWI GGATC 2 cut(s) 488, 504
AcoI YGGCCR 2 cut(s) 71, 525
AcvI CACGTG 1 cut(s) 455
AflIII ACRYGT 1 cut(s) 128
AgsI TTSAA 1 cut(s) 343
Alw21I GWGCWC 1 cut(s) 303
Alw26I GTCTC 2 cut(s) 422, 452
AlwI GGATC 2 cut(s) 488, 504
AoxI GGCC 2 cut(s) 71, 525
ApeKI GCWGC 1 cut(s) 461
AsuHPI GGTGA 1 cut(s) 428
BalI TGGCCA 1 cut(s) 73
BanI GGYRCC 1 cut(s) 170
BbrPI CACGTG 1 cut(s) 455
Bbv12I GWGCWC 1 cut(s) 303
BbvI GCAGC 1 cut(s) 448
BccI CCATC 1 cut(s) 148
BcoDI GTCTC 2 cut(s) 422, 452
BfaI CTAG 2 cut(s) 191, 281
BisI GCNGC 3 cut(s) 270, 462, 525
BlsI GCNGC 3 cut(s) 271, 463, 526
BmiI GGNNCC 1 cut(s) 172
BmsI GCATC 2 cut(s) 251, 448
BoxI GACNNNNGTC 1 cut(s) 444
Bpu10I CCTNAGC 1 cut(s) 264
BsaAI YACGTR 1 cut(s) 455
BsaJI CCNNGG 1 cut(s) 285
Bse3DI GCAATG 2 cut(s) 96, 418
BseDI CCNNGG 1 cut(s) 285
BseGI GGATG 1 cut(s) 541
BseMI GCAATG 2 cut(s) 96, 418
BseRI GAGGAG 1 cut(s) 50
BseXI GCAGC 1 cut(s) 448
Bsh1236I CGCG 2 cut(s) 67, 176
BshFI GGCC 2 cut(s) 73, 527
BshNI GGYRCC 1 cut(s) 170
BsiHKAI GWGCWC 1 cut(s) 303
BslFI GGGAC 1 cut(s) 172
BsmAI GTCTC 2 cut(s) 422, 452
BsmFI GGGAC 1 cut(s) 172
BsnI GGCC 2 cut(s) 73, 527
Bsp1286I GDGCHC 1 cut(s) 303
Bsp143I GATC 3 cut(s) 223, 493, 509
Bsp19I CCATGG 1 cut(s) 285
BspACI CCGC 6 cut(s) 67, 174, 240, 269, 400, 524
BspANI GGCC 2 cut(s) 73, 527
BspFNI CGCG 2 cut(s) 67, 176
BspLI GGNNCC 1 cut(s) 172
BspPI GGATC 2 cut(s) 488, 504
BspT107I GGYRCC 1 cut(s) 170
BsrDI GCAATG 2 cut(s) 96, 418
BssECI CCNNGG 1 cut(s) 285
BssMI GATC 3 cut(s) 223, 493, 509
BssT1I CCWWGG 1 cut(s) 285
Bst4CI ACNGT 2 cut(s) 250, 309
BstAPI GCANNNNNTGC 1 cut(s) 458
BstBAI YACGTR 1 cut(s) 455
BstDEI CTNAG 4 cut(s) 264, 327, 443, 514
BstDSI CCRYGG 1 cut(s) 285
BstF5I GGATG 1 cut(s) 541
BstFNI CGCG 2 cut(s) 67, 176
BstKTI GATC 3 cut(s) 226, 496, 512
BstMAI GTCTC 2 cut(s) 422, 452
BstMBI GATC 3 cut(s) 223, 493, 509
BstMWI GCNNNNNNNGC 3 cut(s) 23, 269, 458
BstNSI RCATGY 1 cut(s) 132
BstPAI GACNNNNGTC 1 cut(s) 444
BstUI CGCG 2 cut(s) 67, 176
BstV1I GCAGC 1 cut(s) 448
BstX2I RGATCY 1 cut(s) 509
BstYI RGATCY 1 cut(s) 509
BsuRI GGCC 2 cut(s) 73, 527
BtgI CCRYGG 1 cut(s) 285
BtgZI GCGATG 1 cut(s) 212
BtsCI GGATG 1 cut(s) 541
CseI GACGC 1 cut(s) 165
CviAII CATG 4 cut(s) 23, 129, 286, 336
CviJI RGCY 8 cut(s) 73, 161, 263, 272, 284, 334, 385, 527
CviKI_1 RGCY 8 cut(s) 73, 161, 263, 272, 284, 334, 385, 527
DdeI CTNAG 4 cut(s) 264, 327, 443, 514
DpnI GATC 3 cut(s) 225, 495, 511
DpnII GATC 3 cut(s) 223, 493, 509
EaeI YGGCCR 2 cut(s) 71, 525
Eco130I CCWWGG 1 cut(s) 285
Eco32I GATATC 1 cut(s) 153
Eco72I CACGTG 1 cut(s) 455
EcoRV GATATC 1 cut(s) 153
EcoT14I CCWWGG 1 cut(s) 285
EcoT22I ATGCAT 1 cut(s) 28
ErhI CCWWGG 1 cut(s) 285
FaeI CATG 4 cut(s) 26, 132, 289, 339
FaqI GGGAC 1 cut(s) 172
FatI CATG 4 cut(s) 22, 128, 285, 335
Fnu4HI GCNGC 3 cut(s) 270, 462, 525
FokI GGATG 1 cut(s) 548
Fsp4HI GCNGC 3 cut(s) 270, 462, 525
FspBI CTAG 2 cut(s) 191, 281
GluI GCNGC 3 cut(s) 270, 462, 525
HaeIII GGCC 2 cut(s) 73, 527
HgaI GACGC 1 cut(s) 165
Hin1II CATG 4 cut(s) 26, 132, 289, 339
HinfI GANTC 1 cut(s) 368
HphI GGTGA 1 cut(s) 428
Hpy188III TCNNGA 2 cut(s) 343, 497
HpyAV CCTTC 4 cut(s) 61, 65, 286, 385
HpyCH4III ACNGT 2 cut(s) 250, 309
HpyCH4IV ACGT 4 cut(s) 218, 454, 531, 554
HpyCH4V TGCA 3 cut(s) 26, 86, 474
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 269, 458
HpyF3I CTNAG 4 cut(s) 264, 327, 443, 514
HpySE526I ACGT 4 cut(s) 218, 454, 531, 554
Hsp92II CATG 4 cut(s) 26, 132, 289, 339
Kzo9I GATC 3 cut(s) 223, 493, 509
LpnPI CCDG 1 cut(s) 510
Lsp1109I GCAGC 1 cut(s) 448
LweI GCATC 2 cut(s) 251, 448
MaeI CTAG 2 cut(s) 191, 281
MaeII ACGT 4 cut(s) 218, 454, 531, 554
MalI GATC 3 cut(s) 225, 495, 511
MboI GATC 3 cut(s) 223, 493, 509
MboII GAAGA 2 cut(s) 206, 209
MflI RGATCY 1 cut(s) 509
MhlI GDGCHC 1 cut(s) 303
MlsI TGGCCA 1 cut(s) 73
MluNI TGGCCA 1 cut(s) 73
MmeI TCCRAC 1 cut(s) 117
MnlI CCTC 2 cut(s) 71, 373
Mox20I TGGCCA 1 cut(s) 73
Mph1103I ATGCAT 1 cut(s) 28
MscI TGGCCA 1 cut(s) 73
Msp20I TGGCCA 1 cut(s) 73
MspA1I CMGCKG 1 cut(s) 400
MvnI CGCG 2 cut(s) 67, 176
MwoI GCNNNNNNNGC 3 cut(s) 23, 269, 458
NcoI CCATGG 1 cut(s) 285
NdeII GATC 3 cut(s) 223, 493, 509
NlaIII CATG 4 cut(s) 26, 132, 289, 339
NlaIV GGNNCC 1 cut(s) 172
NsiI ATGCAT 1 cut(s) 28
NspI RCATGY 1 cut(s) 132
PciI ACATGT 1 cut(s) 128
PfeI GAWTC 1 cut(s) 368
PkrI GCNGC 3 cut(s) 271, 463, 526
PmaCI CACGTG 1 cut(s) 455
PmlI CACGTG 1 cut(s) 455
Ppu21I YACGTR 1 cut(s) 455
PscI ACATGT 1 cut(s) 128
PshAI GACNNNNGTC 1 cut(s) 444
Psp1406I AACGTT 1 cut(s) 218
PspCI CACGTG 1 cut(s) 455
PspN4I GGNNCC 1 cut(s) 172
PsuI RGATCY 1 cut(s) 509
SatI GCNGC 3 cut(s) 270, 462, 525
Sau3AI GATC 3 cut(s) 223, 493, 509
SduI GDGCHC 1 cut(s) 303
SetI ASST 7 cut(s) 33, 53, 221, 444, 457, 534, 557
SfaNI GCATC 2 cut(s) 251, 448
SsiI CCGC 6 cut(s) 67, 174, 240, 269, 400, 524
SspMI CTAG 2 cut(s) 191, 281
StyI CCWWGG 1 cut(s) 285
TaaI ACNGT 2 cut(s) 250, 309
TaiI ACGT 4 cut(s) 221, 457, 534, 557
TaqI TCGA 2 cut(s) 245, 378
TauI GCSGC 2 cut(s) 272, 527
TfiI GAWTC 1 cut(s) 368
TseI GCWGC 1 cut(s) 461
TspDTI ATGAA 1 cut(s) 404
XceI RCATGY 1 cut(s) 132
XspI CTAG 2 cut(s) 191, 281
Zsp2I ATGCAT 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.