pycom05g02210

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0005336
Physical Location & Seq
Reverse (-)
2560716 .. 2561303
588 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g02210.1

Sequence Viewer

Length: 588 bp
ATGGAGTCCCCACTTTCTCATCATTTATTGCTCCTCGCTTGCTTCCTCATATTATTTCTCTCTTCTTCTCCTACACCGGCAGATGCAAGAGCGTCTCCATTAGTTCGGAGTGTTTGCAAGGCAACCCGAGACTCTTTCAGTCAAAACTACACTCTATGCGTAAAAACTCTCTGGTCAGATGCTCGAATTCGATCGTCAGCAGCATCCAATGATCTCAAAGAACTTGCTATAAATATTCTTGAGGTAGCAGGAGAAACTGCACAGGATGCCCAAGGGTTGCTTAACAGTTATCAACCCATGATCAAGAGCAGCAAAGAAACCAAAGCAATTCAACAGTGTGCGCAATCGTACGGATCAGTTGCTTCGTGTTTCCGAGGCACACAAGATGAGGTAAAGGAAGAGGACACCATGGCCAACTATACCGTCGCAAGGGTCTCCGACGACATCGGTGTTTGTGAGAAAGCATTGAGTTCTGATGGGGTGAAGCTTCCTACTACAATATCCACCAGACTCCAGTTGGTCAAGTTGTATAACAACATTGGATACACCATCACTATTCAGTTACTAGGTATCTGGCTCCATCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

196

Amino Acids

21.34

Weight (kDa)

6.41

Isoelectric Point (pI)

45.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 34 - 178 6.4e-13 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 342
AclWI GGATC 1 cut(s) 361
AcoI YGGCCR 1 cut(s) 411
AcsI RAATTY 1 cut(s) 186
AfaI GTAC 1 cut(s) 350
AfiI CCNNNNNNNGG 1 cut(s) 429
AgsI TTSAA 1 cut(s) 332
AluBI AGCT 1 cut(s) 487
AluI AGCT 1 cut(s) 487
Alw26I GTCTC 3 cut(s) 99, 123, 439
AlwI GGATC 1 cut(s) 361
Ama87I CYCGRG 1 cut(s) 126
AoxI GGCC 1 cut(s) 411
ApeKI GCWGC 2 cut(s) 200, 309
ApoI RAATTY 1 cut(s) 186
AspLEI GCGC 1 cut(s) 343
AsuHPI GGTGA 1 cut(s) 493
AvaI CYCGRG 1 cut(s) 126
BalI TGGCCA 1 cut(s) 413
BbvI GCAGC 2 cut(s) 212, 321
BccI CCATC 2 cut(s) 470, 557
BciVI GTATCC 1 cut(s) 536
BclI TGATCA 1 cut(s) 300
BcoDI GTCTC 3 cut(s) 99, 123, 439
BfaI CTAG 2 cut(s) 566, 586
BfuI GTATCC 1 cut(s) 536
BisI GCNGC 2 cut(s) 201, 310
BlsI GCNGC 2 cut(s) 202, 311
BmeT110I CYCGRG 1 cut(s) 126
BmiI GGNNCC 1 cut(s) 578
BmsI GCATC 4 cut(s) 73, 169, 212, 256
BpmI CTGGAG 1 cut(s) 497
BpuEI CTTGAG 1 cut(s) 260
BsaI GGTCTC 1 cut(s) 439
BsaJI CCNNGG 3 cut(s) 271, 373, 408
Bsc4I CCNNNNNNNGG 1 cut(s) 429
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 1 cut(s) 514
BseDI CCNNGG 3 cut(s) 271, 373, 408
BseGI GGATG 2 cut(s) 203, 271
BseLI CCNNNNNNNGG 1 cut(s) 429
BseNI ACTGG 1 cut(s) 514
BseRI GAGGAG 1 cut(s) 23
BseXI GCAGC 2 cut(s) 212, 321
BsgI GTGCAG 1 cut(s) 243
Bsh1285I CGRYCG 1 cut(s) 194
BshFI GGCC 1 cut(s) 413
BsiEI CGRYCG 1 cut(s) 194
BsiHKCI CYCGRG 1 cut(s) 126
BsiSI CCGG 1 cut(s) 77
BsiWI CGTACG 1 cut(s) 348
BslI CCNNNNNNNGG 1 cut(s) 429
BsmAI GTCTC 3 cut(s) 99, 123, 439
BsmBI CGTCTC 1 cut(s) 99
BsnI GGCC 1 cut(s) 413
Bso31I GGTCTC 1 cut(s) 439
BsoBI CYCGRG 1 cut(s) 126
Bsp143I GATC 4 cut(s) 191, 211, 300, 353
Bsp19I CCATGG 1 cut(s) 408
BspANI GGCC 1 cut(s) 413
BspLI GGNNCC 1 cut(s) 578
BspPI GGATC 1 cut(s) 361
BspTNI GGTCTC 1 cut(s) 439
BsrFI RCCGGY 1 cut(s) 76
BsrI ACTGG 1 cut(s) 514
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 3 cut(s) 271, 373, 408
BssMI GATC 4 cut(s) 191, 211, 300, 353
BssT1I CCWWGG 2 cut(s) 271, 408
Bst4CI ACNGT 3 cut(s) 287, 336, 424
Bst6I CTCTTC 2 cut(s) 67, 393
BstAPI GCANNNNNTGC 1 cut(s) 266
BstC8I GCNNGC 1 cut(s) 40
BstDSI CCRYGG 1 cut(s) 408
BstF5I GGATG 2 cut(s) 203, 271
BstHHI GCGC 1 cut(s) 343
BstKTI GATC 4 cut(s) 194, 214, 303, 356
BstMAI GTCTC 3 cut(s) 99, 123, 439
BstMBI GATC 4 cut(s) 191, 211, 300, 353
BstMCI CGRYCG 1 cut(s) 194
BstMWI GCNNNNNNNGC 1 cut(s) 266
BstV1I GCAGC 2 cut(s) 212, 321
BsuI GTATCC 1 cut(s) 536
BsuRI GGCC 1 cut(s) 413
BtgI CCRYGG 1 cut(s) 408
BtsCI GGATG 2 cut(s) 203, 271
BtsIMutI CAGTG 1 cut(s) 341
Cac8I GCNNGC 1 cut(s) 40
CfoI GCGC 1 cut(s) 343
Cfr10I RCCGGY 1 cut(s) 76
CseI GACGC 1 cut(s) 81
Csp6I GTAC 1 cut(s) 349
CviAII CATG 2 cut(s) 298, 409
CviJI RGCY 3 cut(s) 413, 487, 577
CviKI_1 RGCY 3 cut(s) 413, 487, 577
CviQI GTAC 1 cut(s) 349
DpnI GATC 4 cut(s) 193, 213, 302, 355
DpnII GATC 4 cut(s) 191, 211, 300, 353
EaeI YGGCCR 1 cut(s) 411
Eam1104I CTCTTC 2 cut(s) 67, 393
EarI CTCTTC 2 cut(s) 67, 393
Eco130I CCWWGG 2 cut(s) 271, 408
Eco31I GGTCTC 1 cut(s) 439
Eco88I CYCGRG 1 cut(s) 126
EcoRI GAATTC 1 cut(s) 186
EcoT14I CCWWGG 2 cut(s) 271, 408
ErhI CCWWGG 2 cut(s) 271, 408
Esp3I CGTCTC 1 cut(s) 99
FaeI CATG 2 cut(s) 301, 412
FaiI YATR 7 cut(s) 50, 157, 230, 299, 410, 420, 531
FalI AAGNNNNNCTT 2 cut(s) 264, 296
FatI CATG 2 cut(s) 297, 408
FbaI TGATCA 1 cut(s) 300
Fnu4HI GCNGC 2 cut(s) 201, 310
FokI GGATG 2 cut(s) 190, 278
Fsp4HI GCNGC 2 cut(s) 201, 310
FspBI CTAG 2 cut(s) 566, 586
FspI TGCGCA 1 cut(s) 342
GlaI GCGC 1 cut(s) 342
GluI GCNGC 2 cut(s) 201, 310
GsuI CTGGAG 1 cut(s) 497
HaeIII GGCC 1 cut(s) 413
HapII CCGG 1 cut(s) 77
HgaI GACGC 1 cut(s) 81
HhaI GCGC 1 cut(s) 343
Hin1II CATG 2 cut(s) 301, 412
Hin6I GCGC 1 cut(s) 341
HinP1I GCGC 1 cut(s) 341
HindIII AAGCTT 1 cut(s) 485
HinfI GANTC 3 cut(s) 5, 131, 510
HpaII CCGG 1 cut(s) 77
HphI GGTGA 1 cut(s) 493
Hpy188I TCNGA 5 cut(s) 108, 178, 374, 439, 475
Hpy188III TCNNGA 2 cut(s) 239, 304
Hpy99I CGWCG 2 cut(s) 428, 443
HpyCH4III ACNGT 3 cut(s) 287, 336, 424
HpyCH4V TGCA 3 cut(s) 86, 117, 260
HpyF10VI GCNNNNNNNGC 1 cut(s) 266
Hsp92II CATG 2 cut(s) 301, 412
HspAI GCGC 1 cut(s) 341
Ksp22I TGATCA 1 cut(s) 300
Kzo9I GATC 4 cut(s) 191, 211, 300, 353
LmnI GCTCC 2 cut(s) 36, 582
LpnPI CCDG 7 cut(s) 90, 157, 234, 248, 520, 527, 559
Lsp1109I GCAGC 2 cut(s) 212, 321
LweI GCATC 4 cut(s) 73, 169, 212, 256
MaeI CTAG 2 cut(s) 566, 586
MaeIII GTNAC 1 cut(s) 561
MalI GATC 4 cut(s) 193, 213, 302, 355
MboI GATC 4 cut(s) 191, 211, 300, 353
MboII GAAGA 3 cut(s) 54, 57, 410
MlsI TGGCCA 1 cut(s) 413
MluCI AATT 2 cut(s) 186, 327
MluNI TGGCCA 1 cut(s) 413
MlyI GAGTC 3 cut(s) 14, 125, 504
MmeI TCCRAC 1 cut(s) 462
MnlI CCTC 6 cut(s) 44, 56, 235, 368, 382, 394
Mox20I TGGCCA 1 cut(s) 413
MscI TGGCCA 1 cut(s) 413
MseI TTAA 1 cut(s) 282
Msp20I TGGCCA 1 cut(s) 413
MspI CCGG 1 cut(s) 77
MwoI GCNNNNNNNGC 1 cut(s) 266
NcoI CCATGG 1 cut(s) 408
NdeII GATC 4 cut(s) 191, 211, 300, 353
NlaIII CATG 2 cut(s) 301, 412
NlaIV GGNNCC 1 cut(s) 578
NsbI TGCGCA 1 cut(s) 342
Pfl23II CGTACG 1 cut(s) 348
PkrI GCNGC 2 cut(s) 202, 311
Ple19I CGATCG 1 cut(s) 194
PleI GAGTC 3 cut(s) 13, 125, 504
PpsI GAGTC 3 cut(s) 13, 125, 504
PspLI CGTACG 1 cut(s) 348
PspN4I GGNNCC 1 cut(s) 578
PvuI CGATCG 1 cut(s) 194
RsaI GTAC 1 cut(s) 350
RsaNI GTAC 1 cut(s) 349
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 2 cut(s) 201, 310
Sau3AI GATC 4 cut(s) 191, 211, 300, 353
SchI GAGTC 3 cut(s) 14, 125, 504
SetI ASST 4 cut(s) 246, 393, 489, 571
SfaNI GCATC 4 cut(s) 73, 169, 212, 256
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 2 cut(s) 186, 327
SspI AATATT 1 cut(s) 235
SspMI CTAG 2 cut(s) 566, 586
StyI CCWWGG 2 cut(s) 271, 408
TaaI ACNGT 3 cut(s) 287, 336, 424
TaqI TCGA 2 cut(s) 184, 190
TasI AATT 2 cut(s) 186, 327
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TscAI CASTG 1 cut(s) 341
TseI GCWGC 2 cut(s) 200, 309
TspGWI ACGGA 1 cut(s) 366
TspRI CASTG 1 cut(s) 341
XapI RAATTY 1 cut(s) 186
XcmI CCANNNNNNNNNTGG 1 cut(s) 514
XspI CTAG 2 cut(s) 566, 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.