AT4G24640

Invertase pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005336
Physical Location & Seq
Forward (+)
12717788 .. 12719287
1500 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G24640.1

Sequence Viewer

Length: 564 bp
ATGGCAACGTCCTCTGTTCTGTCCACCTTCGCTGCGGCGGCAATTACGTTGCAACTACTCTTATCTCCTGCTTCAGCTTCCCCTCACATGAAATACATTGACGCCATCTGCGATCGTTCCCATGACCAAGCTTTCTGCGTTAAAACTTTGACTACCAATCCCCCTACAGCTGCTCCCATTGGCCTGCTTCCATTGTCGGAGGCTGTGATCAACTTGGCCATATCCCACGCGGAGAAGACAGCGATTTTCGTAGATGAGACTGCGAAGAAAGATCCAACGGTGAAGGTTGCATTTACAGAGTGCCACAAGGCCTACTTGGCCGTGGCAGCTGATCTCAAGAGTGCAAATGTGAAGCTCAAGGCATCCCCCGACACGGCTAACTACGACGTCAGGGCTTCCAGTGACAGCATGAGGCGCGTGAATGAATTGGTTGGGAAAAACACTGACAAGGCTTCAACCACGCTCAAGGAAATGACAGTGCAGATGGAGAAACTACTTGATCTTGCAGCTGGAGCCGCCGATGCCGTTGATGACGACGATGAGAACATGCGCCTTCGCGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

187

Amino Acids

19.94

Weight (kDa)

5.54

Isoelectric Point (pI)

31.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 32 - 126 8.4e-11 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 390
AccII CGCG 3 cut(s) 230, 417, 558
AciI CCGC 4 cut(s) 35, 38, 230, 516
AclWI GGATC 1 cut(s) 266
AcoI YGGCCR 2 cut(s) 216, 318
AcuI CTGAAG 1 cut(s) 57
AcyI GRCGYC 2 cut(s) 102, 387
AfiI CCNNNNNNNGG 1 cut(s) 373
AgsI TTSAA 1 cut(s) 456
AluBI AGCT 6 cut(s) 77, 131, 170, 329, 355, 509
AluI AGCT 6 cut(s) 77, 131, 170, 329, 355, 509
Alw26I GTCTC 1 cut(s) 251
AlwI GGATC 1 cut(s) 266
AoxI GGCC 4 cut(s) 181, 216, 309, 318
ApeKI GCWGC 4 cut(s) 32, 170, 326, 506
AspLEI GCGC 2 cut(s) 417, 552
AsuHPI GGTGA 1 cut(s) 292
BalI TGGCCA 1 cut(s) 218
BbsI GAAGAC 1 cut(s) 242
BbvI GCAGC 4 cut(s) 19, 157, 338, 518
BccI CCATC 2 cut(s) 113, 478
BceAI ACGGC 3 cut(s) 305, 390, 509
BclI TGATCA 1 cut(s) 207
BcoDI GTCTC 1 cut(s) 251
BfmI CTRYAG 1 cut(s) 165
BglI GCCNNNNNGGC 1 cut(s) 317
BisI GCNGC 7 cut(s) 33, 36, 39, 171, 327, 507, 516
BlsI GCNGC 7 cut(s) 34, 37, 40, 172, 328, 508, 517
BmiI GGNNCC 1 cut(s) 514
BmsI GCATC 2 cut(s) 371, 511
BpiI GAAGAC 1 cut(s) 242
BpmI CTGGAG 1 cut(s) 531
BpuEI CTTGAG 3 cut(s) 320, 341, 449
BsaHI GRCGYC 2 cut(s) 102, 387
BsaJI CCNNGG 1 cut(s) 321
BsaXI ACNNNNNCTCC 2 cut(s) 157, 187
Bsc4I CCNNNNNNNGG 1 cut(s) 373
Bse1I ACTGG 1 cut(s) 399
BseDI CCNNGG 1 cut(s) 321
BseGI GGATG 1 cut(s) 362
BseLI CCNNNNNNNGG 1 cut(s) 373
BseNI ACTGG 1 cut(s) 399
BseXI GCAGC 4 cut(s) 19, 157, 338, 518
BsgI GTGCAG 1 cut(s) 500
Bsh1236I CGCG 3 cut(s) 230, 417, 558
Bsh1285I CGRYCG 1 cut(s) 115
BshFI GGCC 4 cut(s) 183, 218, 311, 320
BsiEI CGRYCG 1 cut(s) 115
BslI CCNNNNNNNGG 1 cut(s) 373
BsmAI GTCTC 1 cut(s) 251
BsnI GGCC 4 cut(s) 183, 218, 311, 320
Bsp143I GATC 5 cut(s) 112, 207, 271, 331, 499
BspACI CCGC 4 cut(s) 35, 38, 230, 516
BspANI GGCC 4 cut(s) 183, 218, 311, 320
BspFNI CGCG 3 cut(s) 230, 417, 558
BspLI GGNNCC 1 cut(s) 514
BspPI GGATC 1 cut(s) 266
BsrI ACTGG 1 cut(s) 399
BssECI CCNNGG 1 cut(s) 321
BssMI GATC 5 cut(s) 112, 207, 271, 331, 499
BssNI GRCGYC 2 cut(s) 102, 387
Bst4CI ACNGT 2 cut(s) 280, 478
BstACI GRCGYC 2 cut(s) 102, 387
BstC8I GCNNGC 1 cut(s) 185
BstDSI CCRYGG 1 cut(s) 321
BstF5I GGATG 1 cut(s) 362
BstFNI CGCG 3 cut(s) 230, 417, 558
BstHHI GCGC 2 cut(s) 417, 552
BstKTI GATC 5 cut(s) 115, 210, 274, 334, 502
BstMAI GTCTC 1 cut(s) 251
BstMBI GATC 5 cut(s) 112, 207, 271, 331, 499
BstMCI CGRYCG 1 cut(s) 115
BstMWI GCNNNNNNNGC 7 cut(s) 38, 317, 326, 414, 512, 515, 521
BstNSI RCATGY 1 cut(s) 550
BstSFI CTRYAG 1 cut(s) 165
BstUI CGCG 3 cut(s) 230, 417, 558
BstV1I GCAGC 4 cut(s) 19, 157, 338, 518
BstV2I GAAGAC 1 cut(s) 242
BstX2I RGATCY 1 cut(s) 271
BstYI RGATCY 1 cut(s) 271
BsuRI GGCC 4 cut(s) 183, 218, 311, 320
BtgI CCRYGG 1 cut(s) 321
BtsCI GGATG 1 cut(s) 362
BtsIMutI CAGTG 3 cut(s) 406, 441, 483
Cac8I GCNNGC 1 cut(s) 185
CfoI GCGC 2 cut(s) 417, 552
CseI GACGC 2 cut(s) 110, 547
CviAII CATG 4 cut(s) 88, 122, 409, 547
DpnI GATC 5 cut(s) 114, 209, 273, 333, 501
DpnII GATC 5 cut(s) 112, 207, 271, 331, 499
EaeI YGGCCR 2 cut(s) 216, 318
Eco147I AGGCCT 1 cut(s) 311
Eco57I CTGAAG 1 cut(s) 57
FaeI CATG 4 cut(s) 91, 125, 412, 550
FaiI YATR 5 cut(s) 89, 123, 221, 410, 548
FalI AAGNNNNNCTT 2 cut(s) 299, 331
FatI CATG 4 cut(s) 87, 121, 408, 546
FbaI TGATCA 1 cut(s) 207
Fnu4HI GCNGC 7 cut(s) 33, 36, 39, 171, 327, 507, 516
FokI GGATG 1 cut(s) 349
Fsp4HI GCNGC 7 cut(s) 33, 36, 39, 171, 327, 507, 516
GlaI GCGC 2 cut(s) 416, 551
GluI GCNGC 7 cut(s) 33, 36, 39, 171, 327, 507, 516
GsuI CTGGAG 1 cut(s) 531
HaeIII GGCC 4 cut(s) 183, 218, 311, 320
HgaI GACGC 2 cut(s) 110, 547
HhaI GCGC 2 cut(s) 417, 552
Hin1I GRCGYC 2 cut(s) 102, 387
Hin1II CATG 4 cut(s) 91, 125, 412, 550
Hin6I GCGC 2 cut(s) 415, 550
HinP1I GCGC 2 cut(s) 415, 550
HindIII AAGCTT 1 cut(s) 129
HphI GGTGA 1 cut(s) 292
Hpy166II GTNNAC 1 cut(s) 24
Hpy188I TCNGA 1 cut(s) 199
Hpy188III TCNNGA 1 cut(s) 337
Hpy8I GTNNAC 1 cut(s) 24
Hpy99I CGWCG 2 cut(s) 389, 539
HpyAV CCTTC 3 cut(s) 37, 277, 563
HpyCH4III ACNGT 2 cut(s) 280, 478
HpyCH4IV ACGT 3 cut(s) 8, 47, 387
HpyCH4V TGCA 5 cut(s) 52, 290, 344, 481, 506
HpyF10VI GCNNNNNNNGC 7 cut(s) 38, 317, 326, 414, 512, 515, 521
HpySE526I ACGT 3 cut(s) 8, 47, 387
Hsp92I GRCGYC 2 cut(s) 102, 387
Hsp92II CATG 4 cut(s) 91, 125, 412, 550
HspAI GCGC 2 cut(s) 415, 550
Ksp22I TGATCA 1 cut(s) 207
Kzo9I GATC 5 cut(s) 112, 207, 271, 331, 499
LmnI GCTCC 2 cut(s) 178, 512
LpnPI CCDG 5 cut(s) 81, 197, 376, 412, 495
Lsp1109I GCAGC 4 cut(s) 19, 157, 338, 518
LweI GCATC 2 cut(s) 371, 511
MaeII ACGT 3 cut(s) 8, 47, 387
MaeIII GTNAC 1 cut(s) 401
MalI GATC 5 cut(s) 114, 209, 273, 333, 501
MboI GATC 5 cut(s) 112, 207, 271, 331, 499
MboII GAAGA 2 cut(s) 247, 277
MflI RGATCY 1 cut(s) 271
MlsI TGGCCA 1 cut(s) 218
MluCI AATT 2 cut(s) 42, 425
MluNI TGGCCA 1 cut(s) 218
MmeI TCCRAC 2 cut(s) 177, 299
MnlI CCTC 4 cut(s) 22, 93, 193, 405
Mox20I TGGCCA 1 cut(s) 218
MscI TGGCCA 1 cut(s) 218
MseI TTAA 1 cut(s) 141
Msp20I TGGCCA 1 cut(s) 218
MspA1I CMGCKG 3 cut(s) 170, 329, 509
MvnI CGCG 3 cut(s) 230, 417, 558
MwoI GCNNNNNNNGC 7 cut(s) 38, 317, 326, 414, 512, 515, 521
NdeII GATC 5 cut(s) 112, 207, 271, 331, 499
NlaIII CATG 4 cut(s) 91, 125, 412, 550
NlaIV GGNNCC 1 cut(s) 514
NmuCI GTSAC 1 cut(s) 401
NspI RCATGY 1 cut(s) 550
PceI AGGCCT 1 cut(s) 311
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PkrI GCNGC 7 cut(s) 34, 37, 40, 172, 328, 508, 517
Ple19I CGATCG 1 cut(s) 115
PspN4I GGNNCC 1 cut(s) 514
PsuI RGATCY 1 cut(s) 271
PvuI CGATCG 1 cut(s) 115
PvuII CAGCTG 3 cut(s) 170, 329, 509
SaqAI TTAA 1 cut(s) 141
SatI GCNGC 7 cut(s) 33, 36, 39, 171, 327, 507, 516
Sau3AI GATC 5 cut(s) 112, 207, 271, 331, 499
SfaNI GCATC 2 cut(s) 371, 511
SfcI CTRYAG 1 cut(s) 165
SfiI GGCCNNNNNGGCC 1 cut(s) 317
SmlI CTYRAG 3 cut(s) 335, 356, 464
SmoI CTYRAG 3 cut(s) 335, 356, 464
Sse9I AATT 2 cut(s) 42, 425
SseBI AGGCCT 1 cut(s) 311
SsiI CCGC 4 cut(s) 35, 38, 230, 516
StuI AGGCCT 1 cut(s) 311
TaaI ACNGT 2 cut(s) 280, 478
TaiI ACGT 3 cut(s) 11, 50, 390
TasI AATT 2 cut(s) 42, 425
TauI GCSGC 3 cut(s) 38, 41, 518
Tru1I TTAA 1 cut(s) 141
Tru9I TTAA 1 cut(s) 141
TscAI CASTG 3 cut(s) 406, 448, 483
TseFI GTSAC 1 cut(s) 401
TseI GCWGC 4 cut(s) 32, 170, 326, 506
Tsp45I GTSAC 1 cut(s) 401
TspDTI ATGAA 2 cut(s) 104, 438
TspRI CASTG 3 cut(s) 406, 448, 483
XceI RCATGY 1 cut(s) 550
ZraI GACGTC 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.