MD10G1032700.v1.1

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0005336
Physical Location & Seq
Reverse (-)
4235800 .. 4236324
525 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1032700.v1.1.491

Sequence Viewer

Length: 525 bp
ATGGAGTCCCCAATTCACTGTGTGTTACCATTCTTTTTTACCTCTTTGTTCTTGTTTCTCTCTTCACCTTCACCTATGAATGCATCAACTCAACTGGTTGAAACTGTTTGCAAAAAGACCATCAGTAACTCTGAATGCCTAAAAGCTTTCGACTCCGATCCTCGATCTTGTTCAGCATCGAATTACAAGGAGCTTGCTATAGTAGCTCTTGAGTTAGCTATAGCGAATGCAAAAGATAGCCAAAACTTCATAAACAAGTTGCTCAAGAGTGATCCCAGGAAGCCAATTAAGCTGTGTTCTTATTTTTACGAAGGTGTGGTTGGTTCATTCAATAGCGCCAAAACAGAGATAGATGAGGATCCGTTGACGGCCAACTATGATGTCAAAATTGCAGGCGATGATGCAGGTTACTGTGAAACTGCCTTGAGTTCTGAAGGGGTGAAAGTATCTAAAATATCTACTAGAAACCATGTTGTCGACTTATACAGTAGTATTGGAGATGTTATCACCGACCAACTTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

175

Amino Acids

18.97

Weight (kDa)

4.9

Isoelectric Point (pI)

38.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 32 - 161 4.6e-14 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 395
AccI GTMKAC 1 cut(s) 477
AclWI GGATC 4 cut(s) 152, 266, 353, 366
AcoI YGGCCR 1 cut(s) 369
AcuI CTGAAG 1 cut(s) 453
AdeI CACNNNGTG 1 cut(s) 22
AgsI TTSAA 2 cut(s) 101, 331
AjnI CCWGG 1 cut(s) 275
AjuI GAANNNNNNNTTGG 2 cut(s) 303, 335
AluBI AGCT 5 cut(s) 146, 193, 206, 218, 292
AluI AGCT 5 cut(s) 146, 193, 206, 218, 292
AlwI GGATC 4 cut(s) 152, 266, 353, 366
AoxI GGCC 1 cut(s) 369
AspLEI GCGC 1 cut(s) 338
AsuHPI GGTGA 4 cut(s) 57, 63, 451, 499
BamHI GGATCC 1 cut(s) 358
BccI CCATC 1 cut(s) 128
BceAI ACGGC 1 cut(s) 384
BcgI CGANNNNNNTGC 1 cut(s) 500
BciT130I CCWGG 1 cut(s) 277
BfaI CTAG 1 cut(s) 462
BfmI CTRYAG 2 cut(s) 198, 219
BfoI RGCGCY 1 cut(s) 339
BfuAI ACCTGC 1 cut(s) 395
Bme1390I CCNGG 1 cut(s) 277
BmiI GGNNCC 1 cut(s) 360
BmrFI CCNGG 1 cut(s) 277
BmsI GCATC 3 cut(s) 92, 185, 391
BpuEI CTTGAG 3 cut(s) 230, 248, 445
BsaBI GATNNNNATC 1 cut(s) 357
BsaJI CCNNGG 1 cut(s) 275
Bse1I ACTGG 1 cut(s) 99
Bse8I GATNNNNATC 1 cut(s) 357
BseBI CCWGG 1 cut(s) 277
BseDI CCNNGG 1 cut(s) 275
BseJI GATNNNNATC 1 cut(s) 357
BseNI ACTGG 1 cut(s) 99
BshFI GGCC 1 cut(s) 371
BsmI GAATGC 3 cut(s) 85, 140, 232
BsnI GGCC 1 cut(s) 371
Bsp143I GATC 4 cut(s) 157, 164, 271, 358
BspANI GGCC 1 cut(s) 371
BspLI GGNNCC 1 cut(s) 360
BspMI ACCTGC 1 cut(s) 395
BspPI GGATC 4 cut(s) 152, 266, 353, 366
BsrI ACTGG 1 cut(s) 99
BssECI CCNNGG 1 cut(s) 275
BssMI GATC 4 cut(s) 157, 164, 271, 358
Bst2UI CCWGG 1 cut(s) 277
Bst4CI ACNGT 4 cut(s) 20, 106, 413, 488
Bst6I CTCTTC 1 cut(s) 67
BstC8I GCNNGC 2 cut(s) 195, 394
BstH2I RGCGCY 1 cut(s) 339
BstHHI GCGC 1 cut(s) 338
BstKTI GATC 4 cut(s) 160, 167, 274, 361
BstMBI GATC 4 cut(s) 157, 164, 271, 358
BstMWI GCNNNNNNNGC 2 cut(s) 203, 289
BstNI CCWGG 1 cut(s) 277
BstSCI CCNGG 1 cut(s) 275
BstSFI CTRYAG 2 cut(s) 198, 219
BstX2I RGATCY 1 cut(s) 358
BstYI RGATCY 1 cut(s) 358
BsuRI GGCC 1 cut(s) 371
BtgZI GCGATG 1 cut(s) 411
BtsIMutI CAGTG 1 cut(s) 16
BveI ACCTGC 1 cut(s) 395
Cac8I GCNNGC 2 cut(s) 195, 394
CfoI GCGC 1 cut(s) 338
CviAII CATG 1 cut(s) 470
CviJI RGCY 8 cut(s) 146, 193, 206, 218, 240, 283, 292, 371
CviKI_1 RGCY 8 cut(s) 146, 193, 206, 218, 240, 283, 292, 371
DpnI GATC 4 cut(s) 159, 166, 273, 360
DpnII GATC 4 cut(s) 157, 164, 271, 358
DraIII CACNNNGTG 1 cut(s) 22
EaeI YGGCCR 1 cut(s) 369
Eam1104I CTCTTC 1 cut(s) 67
EarI CTCTTC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 453
EcoRII CCWGG 1 cut(s) 275
EcoT22I ATGCAT 1 cut(s) 85
FaeI CATG 1 cut(s) 473
FaiI YATR 7 cut(s) 77, 200, 221, 251, 378, 471, 484
FatI CATG 1 cut(s) 469
FblI GTMKAC 1 cut(s) 477
FspBI CTAG 1 cut(s) 462
GlaI GCGC 1 cut(s) 337
HaeII RGCGCY 1 cut(s) 339
HaeIII GGCC 1 cut(s) 371
HhaI GCGC 1 cut(s) 338
Hin1II CATG 1 cut(s) 473
Hin6I GCGC 1 cut(s) 336
HinP1I GCGC 1 cut(s) 336
HincII GTYRAC 2 cut(s) 366, 478
HindII GTYRAC 2 cut(s) 366, 478
HindIII AAGCTT 1 cut(s) 144
HinfI GANTC 2 cut(s) 5, 152
HphI GGTGA 4 cut(s) 57, 63, 451, 499
Hpy166II GTNNAC 2 cut(s) 366, 478
Hpy188I TCNGA 3 cut(s) 133, 157, 433
Hpy188III TCNNGA 2 cut(s) 209, 265
Hpy8I GTNNAC 2 cut(s) 366, 478
HpyAV CCTTC 3 cut(s) 78, 305, 428
HpyCH4III ACNGT 4 cut(s) 20, 106, 413, 488
HpyCH4V TGCA 5 cut(s) 83, 111, 230, 392, 404
HpyF10VI GCNNNNNNNGC 2 cut(s) 203, 289
Hsp92II CATG 1 cut(s) 473
HspAI GCGC 1 cut(s) 336
Kzo9I GATC 4 cut(s) 157, 164, 271, 358
LmnI GCTCC 1 cut(s) 190
LpnPI CCDG 5 cut(s) 80, 262, 289, 378, 390
LweI GCATC 3 cut(s) 92, 185, 391
MaeI CTAG 1 cut(s) 462
MaeIII GTNAC 3 cut(s) 24, 125, 407
MalI GATC 4 cut(s) 159, 166, 273, 360
MboI GATC 4 cut(s) 157, 164, 271, 358
MboII GAAGA 1 cut(s) 54
MflI RGATCY 1 cut(s) 358
MluCI AATT 4 cut(s) 12, 181, 285, 387
MlyI GAGTC 2 cut(s) 14, 146
MnlI CCTC 3 cut(s) 52, 171, 349
Mph1103I ATGCAT 1 cut(s) 85
MseI TTAA 1 cut(s) 288
MspR9I CCNGG 1 cut(s) 277
Mva1269I GAATGC 3 cut(s) 85, 140, 232
MvaI CCWGG 1 cut(s) 277
MwoI GCNNNNNNNGC 2 cut(s) 203, 289
NdeII GATC 4 cut(s) 157, 164, 271, 358
NlaIII CATG 1 cut(s) 473
NlaIV GGNNCC 1 cut(s) 360
NsiI ATGCAT 1 cut(s) 85
PctI GAATGC 3 cut(s) 85, 140, 232
PleI GAGTC 2 cut(s) 13, 146
PpsI GAGTC 2 cut(s) 13, 146
Psp6I CCWGG 1 cut(s) 275
PspGI CCWGG 1 cut(s) 275
PspN4I GGNNCC 1 cut(s) 360
PsuI RGATCY 1 cut(s) 358
SalI GTCGAC 1 cut(s) 476
SaqAI TTAA 1 cut(s) 288
Sau3AI GATC 4 cut(s) 157, 164, 271, 358
SchI GAGTC 2 cut(s) 14, 146
ScrFI CCNGG 1 cut(s) 277
SfaNI GCATC 3 cut(s) 92, 185, 391
SfcI CTRYAG 2 cut(s) 198, 219
SmlI CTYRAG 3 cut(s) 209, 263, 424
SmoI CTYRAG 3 cut(s) 209, 263, 424
Sse9I AATT 4 cut(s) 12, 181, 285, 387
SspMI CTAG 1 cut(s) 462
StyD4I CCNGG 1 cut(s) 275
TaaI ACNGT 4 cut(s) 20, 106, 413, 488
TaqI TCGA 4 cut(s) 150, 163, 179, 477
TasI AATT 4 cut(s) 12, 181, 285, 387
Tru1I TTAA 1 cut(s) 288
Tru9I TTAA 1 cut(s) 288
TscAI CASTG 1 cut(s) 23
TspDTI ATGAA 3 cut(s) 92, 238, 315
TspGWI ACGGA 1 cut(s) 351
TspRI CASTG 1 cut(s) 23
XmiI GTMKAC 1 cut(s) 477
XspI CTAG 1 cut(s) 462
Zsp2I ATGCAT 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.