pycom05g02200

Invertase pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0005336
Physical Location & Seq
Reverse (-)
2547291 .. 2547803
513 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g02200.1

Sequence Viewer

Length: 513 bp
ATGGAGTCCCCACTTTCTAATCATGTATTGCTCTTCGCTTGCTTCCTCATATTATTTCTCTCTTCTTCTCCTACACCGGCAGATGCAAGAGCGTCTCCATTAGTTCGGAGTGTTTGCAAGGCAACCCGAGACTCTTTCAGTCAAAACTACACTCTATGCGTAAAAACTCTCTGGTCAGATGCTCGAATTCGATCGTCAGCTGCATCCAATAATCTCAAAGAACTTGCTATAAATATTCTTGAGGTAGCAGCAGAAACTGCTAAAGACGCCCAACAGTTGCTTAACAGTTATCAACCCATGATCGAGAGCAGCAAGGAAACCAAAGGAATTCAACAGTGTGCGCAATCGTACGGATCAGTTGCTTCGTGTTTCCGAGGCACACAAGATGAGGTAGAGGAAGAGGACACCATGGCCAACTATACCGTCGCAAGGGTCTCCGACGATATCGGTGTATGTGAGAAAGCATTGAGTTCTAATGATATCCACCAGACTCCAGTTGGTCAAGTTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

171

Amino Acids

18.49

Weight (kDa)

4.88

Isoelectric Point (pI)

53.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 34 - 159 2.3e-11 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 342
AclWI GGATC 1 cut(s) 361
AcoI YGGCCR 1 cut(s) 411
AcsI RAATTY 2 cut(s) 186, 327
AcyI GRCGYC 1 cut(s) 267
AfaI GTAC 1 cut(s) 350
AfiI CCNNNNNNNGG 1 cut(s) 429
AgsI TTSAA 1 cut(s) 332
AluBI AGCT 1 cut(s) 200
AluI AGCT 1 cut(s) 200
Alw26I GTCTC 3 cut(s) 99, 123, 439
AlwI GGATC 1 cut(s) 361
AlwNI CAGNNNCTG 1 cut(s) 257
Ama87I CYCGRG 1 cut(s) 126
AoxI GGCC 1 cut(s) 411
ApeKI GCWGC 3 cut(s) 200, 248, 309
ApoI RAATTY 2 cut(s) 186, 327
AspLEI GCGC 1 cut(s) 343
AvaI CYCGRG 1 cut(s) 126
BalI TGGCCA 1 cut(s) 413
BbvI GCAGC 3 cut(s) 187, 260, 321
BcoDI GTCTC 3 cut(s) 99, 123, 439
BisI GCNGC 3 cut(s) 201, 249, 310
BlsI GCNGC 3 cut(s) 202, 250, 311
BmeT110I CYCGRG 1 cut(s) 126
BmsI GCATC 3 cut(s) 73, 169, 212
BpmI CTGGAG 1 cut(s) 477
BpuEI CTTGAG 1 cut(s) 260
BsaHI GRCGYC 1 cut(s) 267
BsaI GGTCTC 1 cut(s) 439
BsaJI CCNNGG 2 cut(s) 373, 408
Bsc4I CCNNNNNNNGG 1 cut(s) 429
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 1 cut(s) 494
BseDI CCNNGG 2 cut(s) 373, 408
BseGI GGATG 1 cut(s) 203
BseLI CCNNNNNNNGG 1 cut(s) 429
BseNI ACTGG 1 cut(s) 494
BseXI GCAGC 3 cut(s) 187, 260, 321
Bsh1285I CGRYCG 1 cut(s) 194
BshFI GGCC 1 cut(s) 413
BsiEI CGRYCG 1 cut(s) 194
BsiHKCI CYCGRG 1 cut(s) 126
BsiSI CCGG 1 cut(s) 77
BsiWI CGTACG 1 cut(s) 348
BslI CCNNNNNNNGG 1 cut(s) 429
BsmAI GTCTC 3 cut(s) 99, 123, 439
BsmBI CGTCTC 1 cut(s) 99
BsnI GGCC 1 cut(s) 413
Bso31I GGTCTC 1 cut(s) 439
BsoBI CYCGRG 1 cut(s) 126
Bsp143I GATC 3 cut(s) 191, 300, 353
Bsp19I CCATGG 1 cut(s) 408
BspANI GGCC 1 cut(s) 413
BspPI GGATC 1 cut(s) 361
BspQI GCTCTTC 1 cut(s) 38
BspTNI GGTCTC 1 cut(s) 439
BsrFI RCCGGY 1 cut(s) 76
BsrI ACTGG 1 cut(s) 494
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 2 cut(s) 373, 408
BssMI GATC 3 cut(s) 191, 300, 353
BssNI GRCGYC 1 cut(s) 267
BssT1I CCWWGG 1 cut(s) 408
Bst4CI ACNGT 4 cut(s) 276, 287, 336, 424
Bst6I CTCTTC 3 cut(s) 38, 67, 393
BstACI GRCGYC 1 cut(s) 267
BstAPI GCANNNNNTGC 1 cut(s) 257
BstC8I GCNNGC 1 cut(s) 40
BstDSI CCRYGG 1 cut(s) 408
BstF5I GGATG 1 cut(s) 203
BstHHI GCGC 1 cut(s) 343
BstKTI GATC 3 cut(s) 194, 303, 356
BstMAI GTCTC 3 cut(s) 99, 123, 439
BstMBI GATC 3 cut(s) 191, 300, 353
BstMCI CGRYCG 1 cut(s) 194
BstMWI GCNNNNNNNGC 2 cut(s) 257, 266
BstV1I GCAGC 3 cut(s) 187, 260, 321
BsuRI GGCC 1 cut(s) 413
BtgI CCRYGG 1 cut(s) 408
BtsCI GGATG 1 cut(s) 203
BtsIMutI CAGTG 1 cut(s) 341
Cac8I GCNNGC 1 cut(s) 40
CaiI CAGNNNCTG 1 cut(s) 257
CfoI GCGC 1 cut(s) 343
Cfr10I RCCGGY 1 cut(s) 76
CseI GACGC 2 cut(s) 81, 275
Csp6I GTAC 1 cut(s) 349
CviAII CATG 3 cut(s) 23, 298, 409
CviJI RGCY 2 cut(s) 200, 413
CviKI_1 RGCY 2 cut(s) 200, 413
CviQI GTAC 1 cut(s) 349
DpnI GATC 3 cut(s) 193, 302, 355
DpnII GATC 3 cut(s) 191, 300, 353
EaeI YGGCCR 1 cut(s) 411
Eam1104I CTCTTC 3 cut(s) 38, 67, 393
EarI CTCTTC 3 cut(s) 38, 67, 393
Eco130I CCWWGG 1 cut(s) 408
Eco31I GGTCTC 1 cut(s) 439
Eco32I GATATC 2 cut(s) 445, 481
Eco88I CYCGRG 1 cut(s) 126
EcoRI GAATTC 2 cut(s) 186, 327
EcoRV GATATC 2 cut(s) 445, 481
EcoT14I CCWWGG 1 cut(s) 408
ErhI CCWWGG 1 cut(s) 408
Esp3I CGTCTC 1 cut(s) 99
FaeI CATG 3 cut(s) 26, 301, 412
FaiI YATR 9 cut(s) 24, 50, 157, 230, 299, 410, 420, 454, 511
FatI CATG 3 cut(s) 22, 297, 408
Fnu4HI GCNGC 3 cut(s) 201, 249, 310
FokI GGATG 1 cut(s) 190
Fsp4HI GCNGC 3 cut(s) 201, 249, 310
FspI TGCGCA 1 cut(s) 342
GlaI GCGC 1 cut(s) 342
GluI GCNGC 3 cut(s) 201, 249, 310
GsuI CTGGAG 1 cut(s) 477
HaeIII GGCC 1 cut(s) 413
HapII CCGG 1 cut(s) 77
HgaI GACGC 2 cut(s) 81, 275
HhaI GCGC 1 cut(s) 343
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 3 cut(s) 26, 301, 412
Hin6I GCGC 1 cut(s) 341
HinP1I GCGC 1 cut(s) 341
HinfI GANTC 3 cut(s) 5, 131, 490
HpaII CCGG 1 cut(s) 77
Hpy188I TCNGA 4 cut(s) 108, 178, 374, 439
Hpy188III TCNNGA 2 cut(s) 239, 304
Hpy99I CGWCG 2 cut(s) 428, 443
HpyCH4III ACNGT 4 cut(s) 276, 287, 336, 424
HpyCH4V TGCA 3 cut(s) 86, 117, 203
HpyF10VI GCNNNNNNNGC 2 cut(s) 257, 266
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 3 cut(s) 26, 301, 412
HspAI GCGC 1 cut(s) 341
Kzo9I GATC 3 cut(s) 191, 300, 353
LguI GCTCTTC 1 cut(s) 38
LpnPI CCDG 4 cut(s) 90, 157, 500, 507
Lsp1109I GCAGC 3 cut(s) 187, 260, 321
LweI GCATC 3 cut(s) 73, 169, 212
MalI GATC 3 cut(s) 193, 302, 355
MboI GATC 3 cut(s) 191, 300, 353
MboII GAAGA 4 cut(s) 25, 54, 57, 410
MlsI TGGCCA 1 cut(s) 413
MluCI AATT 2 cut(s) 186, 327
MluNI TGGCCA 1 cut(s) 413
MlyI GAGTC 3 cut(s) 14, 125, 484
MmeI TCCRAC 1 cut(s) 462
MnlI CCTC 6 cut(s) 56, 235, 368, 382, 388, 394
Mox20I TGGCCA 1 cut(s) 413
MscI TGGCCA 1 cut(s) 413
MseI TTAA 1 cut(s) 282
Msp20I TGGCCA 1 cut(s) 413
MspA1I CMGCKG 1 cut(s) 200
MspI CCGG 1 cut(s) 77
MwoI GCNNNNNNNGC 2 cut(s) 257, 266
NcoI CCATGG 1 cut(s) 408
NdeII GATC 3 cut(s) 191, 300, 353
NlaIII CATG 3 cut(s) 26, 301, 412
NsbI TGCGCA 1 cut(s) 342
PciSI GCTCTTC 1 cut(s) 38
Pfl23II CGTACG 1 cut(s) 348
PkrI GCNGC 3 cut(s) 202, 250, 311
Ple19I CGATCG 1 cut(s) 194
PleI GAGTC 3 cut(s) 13, 125, 484
PpsI GAGTC 3 cut(s) 13, 125, 484
PspLI CGTACG 1 cut(s) 348
PstNI CAGNNNCTG 1 cut(s) 257
PvuI CGATCG 1 cut(s) 194
PvuII CAGCTG 1 cut(s) 200
RsaI GTAC 1 cut(s) 350
RsaNI GTAC 1 cut(s) 349
SapI GCTCTTC 1 cut(s) 38
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 3 cut(s) 201, 249, 310
Sau3AI GATC 3 cut(s) 191, 300, 353
SchI GAGTC 3 cut(s) 14, 125, 484
SetI ASST 3 cut(s) 202, 246, 393
SfaNI GCATC 3 cut(s) 73, 169, 212
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 2 cut(s) 186, 327
SspI AATATT 1 cut(s) 235
StyI CCWWGG 1 cut(s) 408
TaaI ACNGT 4 cut(s) 276, 287, 336, 424
TaqI TCGA 3 cut(s) 184, 190, 303
TasI AATT 2 cut(s) 186, 327
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TscAI CASTG 1 cut(s) 341
TseI GCWGC 3 cut(s) 200, 248, 309
TspGWI ACGGA 1 cut(s) 366
TspRI CASTG 1 cut(s) 341
XapI RAATTY 2 cut(s) 186, 327
XcmI CCANNNNNNNNNTGG 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.