Rroxscaffold_7G00194110

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005336
Physical Location & Seq
Reverse (-)
35876770 .. 35877312
543 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00194110.1

Sequence Viewer

Length: 543 bp
ATGGAGTCTCCACTAGGTTTTGCGTTAACCTTGTTCTTTGCTTCCTTGTTCTTACTTCTCTCTTCCCCTTCACCCACAAATGCATCAACTCGTTTAGTTGAATCCGTGTGCAATGAAGTAGAAGGCATTTTGAAAGGAAGCAACTCTGAATGCTTGAAAGCTCTTGAGTTGGATCCTCGAACCGGATCGGCGTCCACTTACGAAGTGCTTGCCCCGATCGCTATTGATTTAGCAATAGCAAATGCAAAGGATAGTGAAACTTTCGTTAAAAATTTGCTCAAGAACAACCCTAGCAGTGAAGCTATAAAACAGTGTGCAGATTCTTATAATATAGTGGTAAGGGATTTTGTAGGTACCAAAGAGGAGCTGAAGGAGGATCCTTTGTCGGCCAACTATGACATCAAAATTGCCGGCGACAATGTCCATGATTGTGAGACCGCATTGAGTTCTAAAGGGCTTCAAGTTCCTGAAATAGCTTCCAGAAACCATGCAATGTTCTTATATAGTAATATTGGATTTGTTATCACTAATCATATTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

180

Amino Acids

19.46

Weight (kDa)

4.71

Isoelectric Point (pI)

44.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 32 - 160 6.6e-11 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 327
Acc65I GGTACC 1 cut(s) 353
AccB1I GGYRCC 1 cut(s) 353
AciI CCGC 1 cut(s) 438
AclWI GGATC 5 cut(s) 167, 180, 193, 371, 384
AcoI YGGCCR 1 cut(s) 387
AcsI RAATTY 1 cut(s) 271
AcuI CTGAAG 1 cut(s) 389
AcyI GRCGYC 1 cut(s) 191
AfaI GTAC 1 cut(s) 355
AfiI CCNNNNNNNGG 1 cut(s) 182
AgsI TTSAA 4 cut(s) 101, 133, 157, 461
AluBI AGCT 4 cut(s) 161, 302, 367, 476
AluI AGCT 4 cut(s) 161, 302, 367, 476
Alw26I GTCTC 2 cut(s) 12, 428
AlwI GGATC 5 cut(s) 167, 180, 193, 371, 384
AoxI GGCC 1 cut(s) 387
ApoI RAATTY 1 cut(s) 271
Asp718I GGTACC 1 cut(s) 353
AsuHPI GGTGA 1 cut(s) 63
BamHI GGATCC 2 cut(s) 172, 376
BanI GGYRCC 1 cut(s) 353
BcgI CGANNNNNNTGC 2 cut(s) 191, 225
BcoDI GTCTC 2 cut(s) 12, 428
BfaI CTAG 2 cut(s) 14, 291
BmiI GGNNCC 3 cut(s) 174, 355, 378
BmsI GCATC 1 cut(s) 92
BpuEI CTTGAG 2 cut(s) 185, 263
BsaHI GRCGYC 1 cut(s) 191
BsaI GGTCTC 1 cut(s) 428
BsaWI WCCGGW 1 cut(s) 182
Bsc4I CCNNNNNNNGG 1 cut(s) 182
Bse118I RCCGGY 1 cut(s) 410
Bse3DI GCAATG 2 cut(s) 118, 498
BseLI CCNNNNNNNGG 1 cut(s) 182
BseMI GCAATG 2 cut(s) 118, 498
BseRI GAGGAG 1 cut(s) 377
BsgI GTGCAG 1 cut(s) 336
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 1 cut(s) 389
BshNI GGYRCC 1 cut(s) 353
BsiEI CGRYCG 1 cut(s) 219
BsiSI CCGG 2 cut(s) 183, 411
BslI CCNNNNNNNGG 1 cut(s) 182
BsmAI GTCTC 2 cut(s) 12, 428
BsmI GAATGC 1 cut(s) 155
BsnI GGCC 1 cut(s) 389
Bso31I GGTCTC 1 cut(s) 428
Bsp143I GATC 4 cut(s) 172, 185, 216, 376
BspACI CCGC 1 cut(s) 438
BspANI GGCC 1 cut(s) 389
BspLI GGNNCC 3 cut(s) 174, 355, 378
BspPI GGATC 5 cut(s) 167, 180, 193, 371, 384
BspT107I GGYRCC 1 cut(s) 353
BspTNI GGTCTC 1 cut(s) 428
BsrDI GCAATG 2 cut(s) 118, 498
BsrFI RCCGGY 1 cut(s) 410
BssAI RCCGGY 1 cut(s) 410
BssMI GATC 4 cut(s) 172, 185, 216, 376
BssNI GRCGYC 1 cut(s) 191
Bst4CI ACNGT 1 cut(s) 312
Bst6I CTCTTC 1 cut(s) 67
BstACI GRCGYC 1 cut(s) 191
BstC8I GCNNGC 2 cut(s) 210, 412
BstKTI GATC 4 cut(s) 175, 188, 219, 379
BstMAI GTCTC 2 cut(s) 12, 428
BstMBI GATC 4 cut(s) 172, 185, 216, 376
BstMCI CGRYCG 1 cut(s) 219
BstMWI GCNNNNNNNGC 1 cut(s) 218
BstX2I RGATCY 2 cut(s) 172, 376
BstYI RGATCY 2 cut(s) 172, 376
BsuRI GGCC 1 cut(s) 389
BtsI GCAGTG 1 cut(s) 301
BtsIMutI CAGTG 2 cut(s) 301, 317
Cac8I GCNNGC 2 cut(s) 210, 412
Cfr10I RCCGGY 1 cut(s) 410
CseI GACGC 1 cut(s) 180
Csp6I GTAC 1 cut(s) 354
CviAII CATG 2 cut(s) 425, 488
CviJI RGCY 6 cut(s) 161, 302, 367, 389, 457, 476
CviKI_1 RGCY 6 cut(s) 161, 302, 367, 389, 457, 476
CviQI GTAC 1 cut(s) 354
DpnI GATC 4 cut(s) 174, 187, 218, 378
DpnII GATC 4 cut(s) 172, 185, 216, 376
EaeI YGGCCR 1 cut(s) 387
Eam1104I CTCTTC 1 cut(s) 67
EarI CTCTTC 1 cut(s) 67
Eco31I GGTCTC 1 cut(s) 428
Eco57I CTGAAG 1 cut(s) 389
EcoT22I ATGCAT 1 cut(s) 85
FaeI CATG 2 cut(s) 428, 491
FaiI YATR 9 cut(s) 305, 327, 332, 396, 426, 489, 502, 504, 534
FatI CATG 2 cut(s) 424, 487
FspBI CTAG 2 cut(s) 14, 291
HaeIII GGCC 1 cut(s) 389
HapII CCGG 2 cut(s) 183, 411
HgaI GACGC 1 cut(s) 180
Hin1I GRCGYC 1 cut(s) 191
Hin1II CATG 2 cut(s) 428, 491
HincII GTYRAC 1 cut(s) 27
HindII GTYRAC 1 cut(s) 27
HinfI GANTC 3 cut(s) 5, 101, 320
HpaI GTTAAC 1 cut(s) 27
HpaII CCGG 2 cut(s) 183, 411
HphI GGTGA 1 cut(s) 63
Hpy166II GTNNAC 2 cut(s) 27, 195
Hpy188I TCNGA 1 cut(s) 148
Hpy188III TCNNGA 4 cut(s) 164, 280, 467, 480
Hpy8I GTNNAC 2 cut(s) 27, 195
HpyAV CCTTC 3 cut(s) 78, 116, 364
HpyCH4III ACNGT 1 cut(s) 312
HpyCH4V TGCA 5 cut(s) 83, 111, 245, 317, 491
HpyF10VI GCNNNNNNNGC 1 cut(s) 218
Hsp92I GRCGYC 1 cut(s) 191
Hsp92II CATG 2 cut(s) 428, 491
KpnI GGTACC 1 cut(s) 357
KroI GCCGGC 1 cut(s) 410
KroNI GCCGGC 1 cut(s) 412
KspAI GTTAAC 1 cut(s) 27
Kzo9I GATC 4 cut(s) 172, 185, 216, 376
LmnI GCTCC 1 cut(s) 364
LpnPI CCDG 4 cut(s) 196, 424, 480, 493
LweI GCATC 1 cut(s) 92
MaeI CTAG 2 cut(s) 14, 291
MalI GATC 4 cut(s) 174, 187, 218, 378
MboI GATC 4 cut(s) 172, 185, 216, 376
MboII GAAGA 1 cut(s) 54
MflI RGATCY 2 cut(s) 172, 376
MluCI AATT 2 cut(s) 271, 405
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 1 cut(s) 150
MnlI CCTC 3 cut(s) 186, 355, 367
Mph1103I ATGCAT 1 cut(s) 85
MroNI GCCGGC 1 cut(s) 410
MseI TTAA 3 cut(s) 26, 267, 541
MslI CAYNNNNRTG 1 cut(s) 429
MspI CCGG 2 cut(s) 183, 411
Mva1269I GAATGC 1 cut(s) 155
MwoI GCNNNNNNNGC 1 cut(s) 218
NaeI GCCGGC 1 cut(s) 412
NdeII GATC 4 cut(s) 172, 185, 216, 376
NgoMIV GCCGGC 1 cut(s) 410
NlaIII CATG 2 cut(s) 428, 491
NlaIV GGNNCC 3 cut(s) 174, 355, 378
NsiI ATGCAT 1 cut(s) 85
PctI GAATGC 1 cut(s) 155
PdiI GCCGGC 1 cut(s) 412
PfeI GAWTC 2 cut(s) 101, 320
PflFI GACNNNGTC 1 cut(s) 419
Ple19I CGATCG 1 cut(s) 219
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
PsiI TTATAA 1 cut(s) 327
PspN4I GGNNCC 3 cut(s) 174, 355, 378
PsuI RGATCY 2 cut(s) 172, 376
PsyI GACNNNGTC 1 cut(s) 419
PvuI CGATCG 1 cut(s) 219
RsaI GTAC 1 cut(s) 355
RsaNI GTAC 1 cut(s) 354
RseI CAYNNNNRTG 1 cut(s) 429
SaqAI TTAA 3 cut(s) 26, 267, 541
Sau3AI GATC 4 cut(s) 172, 185, 216, 376
SchI GAGTC 1 cut(s) 14
SetI ASST 7 cut(s) 19, 32, 163, 304, 355, 369, 478
SfaNI GCATC 1 cut(s) 92
SmiMI CAYNNNNRTG 1 cut(s) 429
SmlI CTYRAG 2 cut(s) 164, 278
SmoI CTYRAG 2 cut(s) 164, 278
Sse9I AATT 2 cut(s) 271, 405
SsiI CCGC 1 cut(s) 438
SspI AATATT 1 cut(s) 511
SspMI CTAG 2 cut(s) 14, 291
TaaI ACNGT 1 cut(s) 312
TaqI TCGA 1 cut(s) 178
TasI AATT 2 cut(s) 271, 405
TfiI GAWTC 2 cut(s) 101, 320
Tru1I TTAA 3 cut(s) 26, 267, 541
Tru9I TTAA 3 cut(s) 26, 267, 541
TscAI CASTG 2 cut(s) 301, 317
TspDTI ATGAA 1 cut(s) 129
TspGWI ACGGA 1 cut(s) 94
TspRI CASTG 2 cut(s) 301, 317
Tth111I GACNNNGTC 1 cut(s) 419
XapI RAATTY 1 cut(s) 271
XspI CTAG 2 cut(s) 14, 291
Zsp2I ATGCAT 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.