AT5G50040

Invertase pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005336
Physical Location & Seq
Forward (+)
20356876 .. 20358381
1506 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50040.1

Sequence Viewer

Length: 528 bp
ATGGCTTCTCCCTCCAACTATTTTTTCCTAGTTTCTCTTGCAGTTCTGTTACCATTTCTCATAATCTTTGCTTCGGCCACAACTTACATTGACGCCATATGTCAAAGTGTTACCGACAAAGCCTTCTGCGCCAAAACTTTGAACGCATACCCTGCCGCGGGCTCTGCCACCAGCCAGTTTCAAGCAGCTACGGCCACGCTCAACCTAGCCATTTCTTATGCCGATAAGTCTGCGGGTTTCACCGGAAATGCGGCAAAGGAGAATCCAACCTTGAAGACGCAATTTGCAGCGAGTCAAGATGCCTTTGTGACCATTAGCAAGTCTCTCAAGAGTGCTGCTTCGGAACTCAAGATATCGCCAGACACTGCCAACTATGATGTCATGGTTTGTAGTGACAGCATAGCGACGGTGAAGAATTTGGTAGAGAAAAATACAGACAATTCGTCAAAGACTGTTATGACGATGACATTGATGATGGAGAAACTTCTTACAATCGCCGTTGGAGCCACTGTCGCTGTTGGCGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

175

Amino Acids

18.27

Weight (kDa)

6.17

Isoelectric Point (pI)

21.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 29 - 137 7.4e-06 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 158
AciI CCGC 5 cut(s) 156, 158, 233, 251, 522
AcoI YGGCCR 2 cut(s) 75, 192
AcsI RAATTY 1 cut(s) 415
AcyI GRCGYC 1 cut(s) 93
AfiI CCNNNNNNNGG 2 cut(s) 157, 158
AgsI TTSAA 3 cut(s) 142, 182, 274
AhdI GACNNNNNGTC 1 cut(s) 442
AjuI GAANNNNNNNTTGG 2 cut(s) 8, 40
AluBI AGCT 1 cut(s) 188
AluI AGCT 1 cut(s) 188
Alw26I GTCTC 1 cut(s) 327
AlwNI CAGNNNCTG 1 cut(s) 365
AoxI GGCC 2 cut(s) 75, 192
ApeKI GCWGC 3 cut(s) 185, 287, 335
ApoI RAATTY 1 cut(s) 415
AspLEI GCGC 1 cut(s) 131
AsuHPI GGTGA 2 cut(s) 232, 421
BanII GRGCYC 1 cut(s) 164
BbsI GAAGAC 1 cut(s) 281
BbvI GCAGC 3 cut(s) 197, 299, 322
BccI CCATC 1 cut(s) 469
BceAI ACGGC 2 cut(s) 207, 482
BcgI CGANNNNNNTGC 2 cut(s) 212, 246
BcoDI GTCTC 1 cut(s) 327
BfaI CTAG 2 cut(s) 29, 206
BisI GCNGC 5 cut(s) 156, 186, 252, 288, 336
BlsI GCNGC 5 cut(s) 157, 187, 253, 289, 337
BmeRI GACNNNNNGTC 1 cut(s) 442
BmiI GGNNCC 1 cut(s) 505
BmsI GCATC 1 cut(s) 289
BpiI GAAGAC 1 cut(s) 281
BpuEI CTTGAG 2 cut(s) 311, 332
BsaHI GRCGYC 1 cut(s) 93
BsaJI CCNNGG 1 cut(s) 156
BsaWI WCCGGW 1 cut(s) 242
BsaXI ACNNNNNCTCC 2 cut(s) 495, 525
Bsc4I CCNNNNNNNGG 2 cut(s) 157, 158
Bse1I ACTGG 1 cut(s) 175
BseDI CCNNGG 1 cut(s) 156
BseLI CCNNNNNNNGG 2 cut(s) 157, 158
BseNI ACTGG 1 cut(s) 175
BseXI GCAGC 3 cut(s) 197, 299, 322
Bsh1236I CGCG 1 cut(s) 158
BshFI GGCC 2 cut(s) 77, 194
BsiSI CCGG 1 cut(s) 243
BslI CCNNNNNNNGG 2 cut(s) 157, 158
BsmAI GTCTC 1 cut(s) 327
BsnI GGCC 2 cut(s) 77, 194
Bsp1286I GDGCHC 1 cut(s) 164
BspACI CCGC 5 cut(s) 156, 158, 233, 251, 522
BspANI GGCC 2 cut(s) 77, 194
BspFNI CGCG 1 cut(s) 158
BspLI GGNNCC 1 cut(s) 505
BsrI ACTGG 1 cut(s) 175
BssECI CCNNGG 1 cut(s) 156
BssNI GRCGYC 1 cut(s) 93
Bst4CI ACNGT 3 cut(s) 409, 454, 511
BstACI GRCGYC 1 cut(s) 93
BstAPI GCANNNNNTGC 1 cut(s) 152
BstC8I GCNNGC 1 cut(s) 160
BstDSI CCRYGG 1 cut(s) 156
BstFNI CGCG 1 cut(s) 158
BstHHI GCGC 1 cut(s) 131
BstMAI GTCTC 1 cut(s) 327
BstMWI GCNNNNNNNGC 6 cut(s) 128, 152, 164, 191, 503, 512
BstUI CGCG 1 cut(s) 158
BstV1I GCAGC 3 cut(s) 197, 299, 322
BstV2I GAAGAC 1 cut(s) 281
BsuRI GGCC 2 cut(s) 77, 194
BtgI CCRYGG 1 cut(s) 156
BtsI GCAGTG 1 cut(s) 363
BtsIMutI CAGTG 2 cut(s) 363, 507
Cac8I GCNNGC 1 cut(s) 160
CaiI CAGNNNCTG 1 cut(s) 365
CfoI GCGC 1 cut(s) 131
Cfr42I CCGCGG 1 cut(s) 159
CseI GACGC 2 cut(s) 101, 286
CviAII CATG 1 cut(s) 382
CviJI RGCY 9 cut(s) 5, 77, 122, 162, 174, 188, 194, 209, 506
CviKI_1 RGCY 9 cut(s) 5, 77, 122, 162, 174, 188, 194, 209, 506
DriI GACNNNNNGTC 1 cut(s) 442
EaeI YGGCCR 2 cut(s) 75, 192
Eam1105I GACNNNNNGTC 1 cut(s) 442
Eco24I GRGCYC 1 cut(s) 164
Eco32I GATATC 1 cut(s) 354
EcoRV GATATC 1 cut(s) 354
EcoT38I GRGCYC 1 cut(s) 164
FaeI CATG 1 cut(s) 385
FaiI YATR 9 cut(s) 62, 98, 100, 148, 219, 375, 383, 401, 458
FatI CATG 1 cut(s) 381
FauI CCCGC 2 cut(s) 151, 226
FauNDI CATATG 1 cut(s) 98
Fnu4HI GCNGC 5 cut(s) 156, 186, 252, 288, 336
FriOI GRGCYC 1 cut(s) 164
Fsp4HI GCNGC 5 cut(s) 156, 186, 252, 288, 336
FspBI CTAG 2 cut(s) 29, 206
GlaI GCGC 1 cut(s) 130
GluI GCNGC 5 cut(s) 156, 186, 252, 288, 336
HaeIII GGCC 2 cut(s) 77, 194
HapII CCGG 1 cut(s) 243
HgaI GACGC 2 cut(s) 101, 286
HhaI GCGC 1 cut(s) 131
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 1 cut(s) 385
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HinfI GANTC 2 cut(s) 262, 292
HpaII CCGG 1 cut(s) 243
HphI GGTGA 2 cut(s) 232, 421
Hpy188I TCNGA 1 cut(s) 343
Hpy188III TCNNGA 3 cut(s) 296, 328, 349
Hpy99I CGWCG 1 cut(s) 409
HpyAV CCTTC 1 cut(s) 133
HpyCH4III ACNGT 3 cut(s) 409, 454, 511
HpyCH4V TGCA 2 cut(s) 41, 287
HpyF10VI GCNNNNNNNGC 6 cut(s) 128, 152, 164, 191, 503, 512
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 1 cut(s) 385
HspAI GCGC 1 cut(s) 129
KspI CCGCGG 1 cut(s) 159
LmnI GCTCC 1 cut(s) 503
LpnPI CCDG 5 cut(s) 165, 184, 188, 256, 372
Lsp1109I GCAGC 3 cut(s) 197, 299, 322
LweI GCATC 1 cut(s) 289
MaeI CTAG 2 cut(s) 29, 206
MaeIII GTNAC 4 cut(s) 48, 109, 307, 392
MboII GAAGA 2 cut(s) 286, 424
MhlI GDGCHC 1 cut(s) 164
MluCI AATT 3 cut(s) 281, 415, 439
MlyI GAGTC 1 cut(s) 301
MmeI TCCRAC 3 cut(s) 39, 290, 481
MnlI CCTC 1 cut(s) 22
MspA1I CMGCKG 1 cut(s) 158
MspI CCGG 1 cut(s) 243
MvnI CGCG 1 cut(s) 158
MwoI GCNNNNNNNGC 6 cut(s) 128, 152, 164, 191, 503, 512
NdeI CATATG 1 cut(s) 98
NlaIII CATG 1 cut(s) 385
NlaIV GGNNCC 1 cut(s) 505
NmuCI GTSAC 2 cut(s) 307, 392
PfeI GAWTC 1 cut(s) 262
PkrI GCNGC 5 cut(s) 157, 187, 253, 289, 337
PleI GAGTC 1 cut(s) 300
PpsI GAGTC 1 cut(s) 300
PspN4I GGNNCC 1 cut(s) 505
PstNI CAGNNNCTG 1 cut(s) 365
SacII CCGCGG 1 cut(s) 159
SatI GCNGC 5 cut(s) 156, 186, 252, 288, 336
SchI GAGTC 1 cut(s) 301
SduI GDGCHC 1 cut(s) 164
SetI ASST 3 cut(s) 190, 207, 272
SfaNI GCATC 1 cut(s) 289
Sfr303I CCGCGG 1 cut(s) 159
SgrBI CCGCGG 1 cut(s) 159
SmlI CTYRAG 2 cut(s) 326, 347
SmoI CTYRAG 2 cut(s) 326, 347
Sse9I AATT 3 cut(s) 281, 415, 439
SsiI CCGC 5 cut(s) 156, 158, 233, 251, 522
SspMI CTAG 2 cut(s) 29, 206
TaaI ACNGT 3 cut(s) 409, 454, 511
TasI AATT 3 cut(s) 281, 415, 439
TauI GCSGC 2 cut(s) 158, 254
TfiI GAWTC 1 cut(s) 262
TscAI CASTG 2 cut(s) 370, 514
TseFI GTSAC 2 cut(s) 307, 392
TseI GCWGC 3 cut(s) 185, 287, 335
Tsp45I GTSAC 2 cut(s) 307, 392
TspRI CASTG 2 cut(s) 370, 514
XapI RAATTY 1 cut(s) 415
XspI CTAG 2 cut(s) 29, 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.