Rh6DG193700

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0005336
Physical Location & Seq
Forward (+)
34633240 .. 34661443
28204 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG193700.1

Sequence Viewer

Length: 582 bp
ATGGAGTCTCCACTAGGTTTTGCGTCAACCTTGTTCTTTGCTTCCCTGTTCTTGCTTCTCTCTTCCCCTTCACCCACAAATGCATCAACTCGTTTAGTTGAATCTGTGTGCAATGAAGTAGTAGGCATTTTGAAAGGCAGCAACTCTGAATGCTTGAAAGCTCTTGAGTTGGATCCTCGAACCGGATCGGCGTCCACTTACGAAGTGCTTGCCCCGATCGCTATTGATTTAGCAATAGCAAATGCAAAGGATAGCGAAGCTTTCATTAAAAATTTGCTCAAGAACAACTCTAGCAGTGAAGCTATAAAACAGTGTGCAGATTCATATAATATAGTGGTAAGGGATTTTGTAGGTGCCAAAGAGGAGCTGAAGGAGGACCCTTTGTCGGCCAACTATGACATCAAAATTGCCGGCGACAATGTCCGTGATTGTGAGAACGCATTGAGTTCTAAAGGGCTTCAAGTAAGAAGTTTAACTATGGTTAAGGGGAGTAACCAAAGCGTAATTTATATCACAAATCGCAGTCAAACAAATACCCTGTTACACCATGAAGCACAAATTGATGCTTACAGCTTCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

193

Amino Acids

20.88

Weight (kDa)

5.08

Isoelectric Point (pI)

37.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 32 - 158 1.7e-11 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 353
AclWI GGATC 3 cut(s) 167, 180, 193
AcoI YGGCCR 1 cut(s) 387
AcsI RAATTY 1 cut(s) 271
AcuI CTGAAG 1 cut(s) 389
AcyI GRCGYC 1 cut(s) 191
AfiI CCNNNNNNNGG 2 cut(s) 182, 385
AgsI TTSAA 4 cut(s) 101, 133, 157, 461
AhdI GACNNNNNGTC 1 cut(s) 382
AluBI AGCT 5 cut(s) 161, 260, 302, 367, 573
AluI AGCT 5 cut(s) 161, 260, 302, 367, 573
Alw26I GTCTC 1 cut(s) 12
AlwI GGATC 3 cut(s) 167, 180, 193
AoxI GGCC 1 cut(s) 387
ApeKI GCWGC 1 cut(s) 138
ApoI RAATTY 1 cut(s) 271
AspS9I GGNCC 1 cut(s) 376
AsuHPI GGTGA 1 cut(s) 63
AvaII GGWCC 1 cut(s) 376
BamHI GGATCC 1 cut(s) 172
BanI GGYRCC 1 cut(s) 353
BbvI GCAGC 1 cut(s) 150
BcgI CGANNNNNNTGC 2 cut(s) 191, 225
BcoDI GTCTC 1 cut(s) 12
BfaI CTAG 2 cut(s) 14, 291
BisI GCNGC 1 cut(s) 139
BlsI GCNGC 1 cut(s) 140
Bme18I GGWCC 1 cut(s) 376
BmeRI GACNNNNNGTC 1 cut(s) 382
BmgT120I GGNCC 1 cut(s) 376
BmiI GGNNCC 3 cut(s) 174, 355, 378
BmsI GCATC 2 cut(s) 92, 553
BpuEI CTTGAG 2 cut(s) 185, 263
BsaHI GRCGYC 1 cut(s) 191
BsaWI WCCGGW 1 cut(s) 182
Bsc4I CCNNNNNNNGG 2 cut(s) 182, 385
Bse118I RCCGGY 1 cut(s) 410
Bse3DI GCAATG 1 cut(s) 118
BseLI CCNNNNNNNGG 2 cut(s) 182, 385
BseMI GCAATG 1 cut(s) 118
BseRI GAGGAG 1 cut(s) 377
BseXI GCAGC 1 cut(s) 150
BsgI GTGCAG 1 cut(s) 336
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 1 cut(s) 389
BshNI GGYRCC 1 cut(s) 353
BsiEI CGRYCG 1 cut(s) 219
BsiSI CCGG 2 cut(s) 183, 411
BslI CCNNNNNNNGG 2 cut(s) 182, 385
BsmAI GTCTC 1 cut(s) 12
BsmI GAATGC 1 cut(s) 155
BsnI GGCC 1 cut(s) 389
Bsp143I GATC 3 cut(s) 172, 185, 216
BspANI GGCC 1 cut(s) 389
BspLI GGNNCC 3 cut(s) 174, 355, 378
BspPI GGATC 3 cut(s) 167, 180, 193
BspT107I GGYRCC 1 cut(s) 353
BsrDI GCAATG 1 cut(s) 118
BsrFI RCCGGY 1 cut(s) 410
BssAI RCCGGY 1 cut(s) 410
BssMI GATC 3 cut(s) 172, 185, 216
BssNI GRCGYC 1 cut(s) 191
Bst4CI ACNGT 1 cut(s) 312
Bst6I CTCTTC 1 cut(s) 67
BstACI GRCGYC 1 cut(s) 191
BstC8I GCNNGC 2 cut(s) 210, 412
BstKTI GATC 3 cut(s) 175, 188, 219
BstMAI GTCTC 1 cut(s) 12
BstMBI GATC 3 cut(s) 172, 185, 216
BstMCI CGRYCG 1 cut(s) 219
BstMWI GCNNNNNNNGC 1 cut(s) 218
BstV1I GCAGC 1 cut(s) 150
BstX2I RGATCY 1 cut(s) 172
BstYI RGATCY 1 cut(s) 172
BsuRI GGCC 1 cut(s) 389
BtsI GCAGTG 1 cut(s) 301
BtsIMutI CAGTG 2 cut(s) 301, 317
Cac8I GCNNGC 2 cut(s) 210, 412
Cfr10I RCCGGY 1 cut(s) 410
Cfr13I GGNCC 1 cut(s) 376
CseI GACGC 2 cut(s) 12, 180
CviAII CATG 1 cut(s) 548
CviJI RGCY 7 cut(s) 161, 260, 302, 367, 389, 457, 573
CviKI_1 RGCY 7 cut(s) 161, 260, 302, 367, 389, 457, 573
DpnI GATC 3 cut(s) 174, 187, 218
DpnII GATC 3 cut(s) 172, 185, 216
DriI GACNNNNNGTC 1 cut(s) 382
EaeI YGGCCR 1 cut(s) 387
Eam1104I CTCTTC 1 cut(s) 67
Eam1105I GACNNNNNGTC 1 cut(s) 382
EarI CTCTTC 1 cut(s) 67
Eco47I GGWCC 1 cut(s) 376
Eco57I CTGAAG 1 cut(s) 389
EcoO109I RGGNCCY 1 cut(s) 376
EcoT22I ATGCAT 1 cut(s) 85
FaeI CATG 1 cut(s) 551
FaiI YATR 8 cut(s) 305, 325, 327, 332, 396, 479, 510, 549
FatI CATG 1 cut(s) 547
Fnu4HI GCNGC 1 cut(s) 139
Fsp4HI GCNGC 1 cut(s) 139
FspBI CTAG 2 cut(s) 14, 291
GluI GCNGC 1 cut(s) 139
HaeIII GGCC 1 cut(s) 389
HapII CCGG 2 cut(s) 183, 411
HgaI GACGC 2 cut(s) 12, 180
Hin1I GRCGYC 1 cut(s) 191
Hin1II CATG 1 cut(s) 551
HincII GTYRAC 1 cut(s) 27
HindII GTYRAC 1 cut(s) 27
HindIII AAGCTT 1 cut(s) 258
HinfI GANTC 3 cut(s) 5, 101, 320
HpaII CCGG 2 cut(s) 183, 411
HphI GGTGA 1 cut(s) 63
Hpy166II GTNNAC 2 cut(s) 27, 195
Hpy188I TCNGA 1 cut(s) 148
Hpy188III TCNNGA 2 cut(s) 164, 280
Hpy8I GTNNAC 2 cut(s) 27, 195
HpyAV CCTTC 2 cut(s) 78, 364
HpyCH4III ACNGT 1 cut(s) 312
HpyCH4V TGCA 4 cut(s) 83, 111, 245, 317
HpyF10VI GCNNNNNNNGC 1 cut(s) 218
Hsp92I GRCGYC 1 cut(s) 191
Hsp92II CATG 1 cut(s) 551
KroI GCCGGC 1 cut(s) 410
KroNI GCCGGC 1 cut(s) 412
Kzo9I GATC 3 cut(s) 172, 185, 216
LmnI GCTCC 1 cut(s) 364
LpnPI CCDG 4 cut(s) 59, 196, 424, 551
Lsp1109I GCAGC 1 cut(s) 150
LweI GCATC 2 cut(s) 92, 553
MaeI CTAG 2 cut(s) 14, 291
MaeIII GTNAC 2 cut(s) 491, 540
MalI GATC 3 cut(s) 174, 187, 218
MboI GATC 3 cut(s) 172, 185, 216
MboII GAAGA 1 cut(s) 54
MflI RGATCY 1 cut(s) 172
MluCI AATT 4 cut(s) 271, 405, 504, 558
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 1 cut(s) 150
MnlI CCTC 3 cut(s) 186, 355, 367
Mph1103I ATGCAT 1 cut(s) 85
MroNI GCCGGC 1 cut(s) 410
MseI TTAA 3 cut(s) 267, 473, 483
MspI CCGG 2 cut(s) 183, 411
Mva1269I GAATGC 1 cut(s) 155
MwoI GCNNNNNNNGC 1 cut(s) 218
NaeI GCCGGC 1 cut(s) 412
NdeII GATC 3 cut(s) 172, 185, 216
NgoMIV GCCGGC 1 cut(s) 410
NlaIII CATG 1 cut(s) 551
NlaIV GGNNCC 3 cut(s) 174, 355, 378
NsiI ATGCAT 1 cut(s) 85
PctI GAATGC 1 cut(s) 155
PdiI GCCGGC 1 cut(s) 412
PfeI GAWTC 2 cut(s) 101, 320
PflFI GACNNNGTC 1 cut(s) 419
PkrI GCNGC 1 cut(s) 140
Ple19I CGATCG 1 cut(s) 219
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
PpuMI RGGWCCY 1 cut(s) 376
Psp5II RGGWCCY 1 cut(s) 376
PspN4I GGNNCC 3 cut(s) 174, 355, 378
PspPI GGNCC 1 cut(s) 376
PspPPI RGGWCCY 1 cut(s) 376
PsuI RGATCY 1 cut(s) 172
PsyI GACNNNGTC 1 cut(s) 419
PvuI CGATCG 1 cut(s) 219
SaqAI TTAA 3 cut(s) 267, 473, 483
SatI GCNGC 1 cut(s) 139
Sau3AI GATC 3 cut(s) 172, 185, 216
Sau96I GGNCC 1 cut(s) 376
SchI GAGTC 1 cut(s) 14
SetI ASST 8 cut(s) 19, 32, 163, 262, 304, 355, 369, 575
SfaNI GCATC 2 cut(s) 92, 553
SinI GGWCC 1 cut(s) 376
SmlI CTYRAG 2 cut(s) 164, 278
SmoI CTYRAG 2 cut(s) 164, 278
Sse9I AATT 4 cut(s) 271, 405, 504, 558
SspMI CTAG 2 cut(s) 14, 291
TaaI ACNGT 1 cut(s) 312
TaqI TCGA 1 cut(s) 178
TasI AATT 4 cut(s) 271, 405, 504, 558
TfiI GAWTC 2 cut(s) 101, 320
Tru1I TTAA 3 cut(s) 267, 473, 483
Tru9I TTAA 3 cut(s) 267, 473, 483
TscAI CASTG 2 cut(s) 301, 317
TseI GCWGC 1 cut(s) 138
TspDTI ATGAA 4 cut(s) 129, 253, 312, 564
TspGWI ACGGA 1 cut(s) 413
TspRI CASTG 2 cut(s) 301, 317
Tth111I GACNNNGTC 1 cut(s) 419
VpaK11BI GGWCC 1 cut(s) 376
XapI RAATTY 1 cut(s) 271
XspI CTAG 2 cut(s) 14, 291
Zsp2I ATGCAT 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.