AT5G50030

Invertase pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005336
Physical Location & Seq
Forward (+)
20354589 .. 20355706
1118 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50030.1

Sequence Viewer

Length: 564 bp
ATGGCTTCTTACTCCGGTTGTTTCTTCGTCGTTACTTTGGCAGTTCTGTCACAATTTCTCTTAGCCCCAGCTTCAGCCTTGGTCAACAGGGAATACATCGATTCCAATTGCCAAAGAGTCAAAAACAAAGCCTTCTGCATCCAAACTTTGACCACCTATCCTCCTGCCGCTGCTGCCACCGGCCTGTTACCATTGGCTGAGGCCGTGGTTGGCCTTGCAATATCCCACTGCGAGAAGACAGCTGGTTTCGCAGCAGAGACAGCCAAAAAGGACGCAACATTGAAGACTCAGTTCAACGAGTGCCACGACGCCTATGTTGGTATTTTGGCGTCTCTAAAGAGCGCTCTACTGGAACTCAAGGACTCACCAGACACTGCCAACTACGACGTCATGGTTTCTGGCGATGATACAAGGCGTGTGAAGGGTTTGGTCGAGAAAAACACTGACACTGCTTCCAAGACGCTTATGGAGATGACACTGCAGATGGAGCAGCTTCTTGATCTCGCTGCTGGAGCCACCGATGCTGCTGACGACGACGATGAGAACTTACATCGTCGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

187

Amino Acids

20.06

Weight (kDa)

4.82

Isoelectric Point (pI)

27.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 32 - 124 2.8e-10 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 390
AciI CCGC 1 cut(s) 168
AcuI CTGAAG 1 cut(s) 57
AcyI GRCGYC 3 cut(s) 309, 329, 387
AfeI AGCGCT 1 cut(s) 343
AgsI TTSAA 2 cut(s) 283, 295
AluBI AGCT 3 cut(s) 71, 242, 493
AluI AGCT 3 cut(s) 71, 242, 493
Alw26I GTCTC 2 cut(s) 251, 336
AlwNI CAGNNNCTG 1 cut(s) 374
Aor51HI AGCGCT 1 cut(s) 343
AoxI GGCC 3 cut(s) 181, 201, 211
ApeKI GCWGC 6 cut(s) 170, 173, 251, 490, 506, 524
AspLEI GCGC 1 cut(s) 344
AsuHPI GGTGA 1 cut(s) 357
BbsI GAAGAC 2 cut(s) 242, 290
BbvCI CCTCAGC 1 cut(s) 198
BbvI GCAGC 6 cut(s) 157, 160, 263, 493, 502, 511
BccI CCATC 1 cut(s) 478
BceAI ACGGC 1 cut(s) 188
BcoDI GTCTC 2 cut(s) 251, 336
BfmI CTRYAG 1 cut(s) 479
BfoI RGCGCY 1 cut(s) 345
BisI GCNGC 7 cut(s) 168, 171, 174, 252, 491, 507, 525
BlsI GCNGC 7 cut(s) 169, 172, 175, 253, 492, 508, 526
BmiI GGNNCC 1 cut(s) 514
BmsI GCATC 2 cut(s) 147, 511
BpiI GAAGAC 2 cut(s) 242, 290
BpmI CTGGAG 1 cut(s) 531
Bpu10I CCTNAGC 1 cut(s) 198
BpuEI CTTGAG 1 cut(s) 341
Bsa29I ATCGAT 1 cut(s) 99
BsaHI GRCGYC 3 cut(s) 309, 329, 387
BsaJI CCNNGG 2 cut(s) 78, 204
BsaWI WCCGGW 1 cut(s) 14
BsaXI ACNNNNNCTCC 2 cut(s) 145, 175
Bse118I RCCGGY 1 cut(s) 179
Bse1I ACTGG 1 cut(s) 354
BseCI ATCGAT 1 cut(s) 99
BseDI CCNNGG 2 cut(s) 78, 204
BseGI GGATG 1 cut(s) 138
BseMII CTCAG 2 cut(s) 189, 302
BseNI ACTGG 1 cut(s) 354
BseXI GCAGC 6 cut(s) 157, 160, 263, 493, 502, 511
BseYI CCCAGC 1 cut(s) 67
BshFI GGCC 3 cut(s) 183, 203, 213
BshVI ATCGAT 1 cut(s) 99
BsiSI CCGG 2 cut(s) 15, 180
BsmAI GTCTC 2 cut(s) 251, 336
BsmBI CGTCTC 1 cut(s) 336
BsnI GGCC 3 cut(s) 183, 203, 213
Bsp143I GATC 1 cut(s) 499
BspACI CCGC 1 cut(s) 168
BspANI GGCC 3 cut(s) 183, 203, 213
BspCNI CTCAG 2 cut(s) 190, 301
BspDI ATCGAT 1 cut(s) 99
BspLI GGNNCC 1 cut(s) 514
BspMAI CTGCAG 1 cut(s) 483
BsrFI RCCGGY 1 cut(s) 179
BsrI ACTGG 1 cut(s) 354
BssAI RCCGGY 1 cut(s) 179
BssECI CCNNGG 2 cut(s) 78, 204
BssMI GATC 1 cut(s) 499
BssNI GRCGYC 3 cut(s) 309, 329, 387
BssT1I CCWWGG 1 cut(s) 78
BstACI GRCGYC 3 cut(s) 309, 329, 387
BstDEI CTNAG 3 cut(s) 61, 198, 288
BstDSI CCRYGG 1 cut(s) 204
BstF5I GGATG 1 cut(s) 138
BstH2I RGCGCY 1 cut(s) 345
BstHHI GCGC 1 cut(s) 344
BstKTI GATC 1 cut(s) 502
BstMAI GTCTC 2 cut(s) 251, 336
BstMBI GATC 1 cut(s) 499
BstMWI GCNNNNNNNGC 6 cut(s) 173, 248, 260, 487, 512, 521
BstSFI CTRYAG 1 cut(s) 479
BstV1I GCAGC 6 cut(s) 157, 160, 263, 493, 502, 511
BstV2I GAAGAC 2 cut(s) 242, 290
Bsu15I ATCGAT 1 cut(s) 99
BsuRI GGCC 3 cut(s) 183, 203, 213
BsuTUI ATCGAT 1 cut(s) 99
BtgI CCRYGG 1 cut(s) 204
BtgZI GCGATG 1 cut(s) 417
BtsCI GGATG 1 cut(s) 138
BtsI GCAGTG 4 cut(s) 226, 372, 447, 476
BtsIMutI CAGTG 5 cut(s) 226, 372, 441, 447, 476
CaiI CAGNNNCTG 1 cut(s) 374
CfoI GCGC 1 cut(s) 344
Cfr10I RCCGGY 1 cut(s) 179
ClaI ATCGAT 1 cut(s) 99
CseI GACGC 4 cut(s) 281, 317, 318, 469
CviAII CATG 1 cut(s) 391
DdeI CTNAG 3 cut(s) 61, 198, 288
DpnI GATC 1 cut(s) 501
DpnII GATC 1 cut(s) 499
Eco130I CCWWGG 1 cut(s) 78
Eco47III AGCGCT 1 cut(s) 343
Eco57I CTGAAG 1 cut(s) 57
EcoT14I CCWWGG 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 78
Esp3I CGTCTC 1 cut(s) 336
FaeI CATG 1 cut(s) 394
FaiI YATR 3 cut(s) 315, 392, 467
FatI CATG 1 cut(s) 390
Fnu4HI GCNGC 7 cut(s) 168, 171, 174, 252, 491, 507, 525
FokI GGATG 1 cut(s) 125
Fsp4HI GCNGC 7 cut(s) 168, 171, 174, 252, 491, 507, 525
GlaI GCGC 1 cut(s) 343
GluI GCNGC 7 cut(s) 168, 171, 174, 252, 491, 507, 525
GsaI CCCAGC 1 cut(s) 71
GsuI CTGGAG 1 cut(s) 531
HaeII RGCGCY 1 cut(s) 345
HaeIII GGCC 3 cut(s) 183, 203, 213
HapII CCGG 2 cut(s) 15, 180
HgaI GACGC 4 cut(s) 281, 317, 318, 469
HhaI GCGC 1 cut(s) 344
Hin1I GRCGYC 3 cut(s) 309, 329, 387
Hin1II CATG 1 cut(s) 394
Hin6I GCGC 1 cut(s) 342
HinP1I GCGC 1 cut(s) 342
HincII GTYRAC 1 cut(s) 85
HindII GTYRAC 1 cut(s) 85
HinfI GANTC 4 cut(s) 101, 117, 286, 362
HpaII CCGG 2 cut(s) 15, 180
HphI GGTGA 1 cut(s) 357
Hpy166II GTNNAC 1 cut(s) 85
Hpy188III TCNNGA 2 cut(s) 433, 497
Hpy8I GTNNAC 1 cut(s) 85
Hpy99I CGWCG 6 cut(s) 32, 311, 389, 536, 539, 558
HpyAV CCTTC 2 cut(s) 142, 415
HpyCH4IV ACGT 1 cut(s) 387
HpyCH4V TGCA 3 cut(s) 138, 218, 481
HpyF10VI GCNNNNNNNGC 6 cut(s) 173, 248, 260, 487, 512, 521
HpyF3I CTNAG 3 cut(s) 61, 198, 288
HpySE526I ACGT 1 cut(s) 387
Hsp92I GRCGYC 3 cut(s) 309, 329, 387
Hsp92II CATG 1 cut(s) 394
HspAI GCGC 1 cut(s) 342
Kzo9I GATC 1 cut(s) 499
LmnI GCTCC 2 cut(s) 487, 512
Lsp1109I GCAGC 6 cut(s) 157, 160, 263, 493, 502, 511
LweI GCATC 2 cut(s) 147, 511
MaeII ACGT 1 cut(s) 387
MaeIII GTNAC 3 cut(s) 31, 48, 186
MalI GATC 1 cut(s) 501
MboI GATC 1 cut(s) 499
MboII GAAGA 3 cut(s) 16, 247, 295
MfeI CAATTG 1 cut(s) 106
MluCI AATT 2 cut(s) 53, 106
MlyI GAGTC 3 cut(s) 126, 280, 356
MnlI CCTC 2 cut(s) 171, 193
MslI CAYNNNNRTG 1 cut(s) 555
MspA1I CMGCKG 2 cut(s) 170, 242
MspI CCGG 2 cut(s) 15, 180
MunI CAATTG 1 cut(s) 106
MwoI GCNNNNNNNGC 6 cut(s) 173, 248, 260, 487, 512, 521
NdeII GATC 1 cut(s) 499
NlaIII CATG 1 cut(s) 394
NlaIV GGNNCC 1 cut(s) 514
NmuCI GTSAC 1 cut(s) 48
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PfeI GAWTC 1 cut(s) 101
PkrI GCNGC 7 cut(s) 169, 172, 175, 253, 492, 508, 526
PleI GAGTC 3 cut(s) 125, 280, 356
PpsI GAGTC 3 cut(s) 125, 280, 356
PspFI CCCAGC 1 cut(s) 67
PspN4I GGNNCC 1 cut(s) 514
PstI CTGCAG 1 cut(s) 483
PstNI CAGNNNCTG 1 cut(s) 374
PvuII CAGCTG 1 cut(s) 242
RseI CAYNNNNRTG 1 cut(s) 555
SatI GCNGC 7 cut(s) 168, 171, 174, 252, 491, 507, 525
Sau3AI GATC 1 cut(s) 499
SchI GAGTC 3 cut(s) 126, 280, 356
SetI ASST 5 cut(s) 73, 158, 244, 390, 495
SfaNI GCATC 2 cut(s) 147, 511
SfcI CTRYAG 1 cut(s) 479
SmiMI CAYNNNNRTG 1 cut(s) 555
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
Sse9I AATT 2 cut(s) 53, 106
SsiI CCGC 1 cut(s) 168
StyI CCWWGG 1 cut(s) 78
TaiI ACGT 1 cut(s) 390
TaqI TCGA 2 cut(s) 99, 432
TasI AATT 2 cut(s) 53, 106
TauI GCSGC 1 cut(s) 170
TfiI GAWTC 1 cut(s) 101
TscAI CASTG 5 cut(s) 233, 379, 448, 454, 483
TseFI GTSAC 1 cut(s) 48
TseI GCWGC 6 cut(s) 170, 173, 251, 490, 506, 524
Tsp45I GTSAC 1 cut(s) 48
TspRI CASTG 5 cut(s) 233, 379, 448, 454, 483
XcmI CCANNNNNNNNNTGG 1 cut(s) 463
ZraI GACGTC 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.