pycom03g09400

DNA polymerase epsilon catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0005336
Physical Location & Seq
Reverse (-)
7910728 .. 7911294
567 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g09400.1

Sequence Viewer

Length: 567 bp
ATGGCCTCCTCAGTCAGTTATGCAGTGTTCATTGCTTCCTTGCTCTTATGCCTCTCGTCATCTCCAGCACCATTTGCAAATGCAAGAGCGTCTCGATTAGTTAGGAGTGTTTGCAAGCAAACCCAAGAACAATTCGGCTATAGGTATAGGCAGTGCGTAAAATCTCTTTGGAAAGATATTCCAATTAGATCGGCAACTAATTTCAAAGATCTTGATATAGCCGTTCTTAAATTAGCAGGAGCAAGTGCAGCACAAACCAAAGCTACGTTTGAAAAAGCTTTCAACGCCACCGACAAGAATGCTAATGGCACGGCAGCCATAAAGCAGTGTGTAGATTCGTATGATTTTGCGTTAGGGTCTTTTGTTTTCGCACTGCAAGGGGTCAATGACGGTGACAAATCGGTTACCGAAATCCTCACACAGGCCCAAGATGACCTTGTTCGTTGCCAAAGAGCATTGGCCTCTGTTGAAGTTCAGCTTCCTATGTCAGTGTCGACGACGAACTTTTGGGTCATGTTATATAGGGATGTTGCATTCTTTGTTACTTCCCAGTTATTAAATATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000228 GO:0000278 GO:0000731 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0004529 GO:0004536 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006271 GO:0006272 GO:0006281 GO:0006284 GO:0006287 GO:0006289 GO:0006297 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008150 GO:0008152 GO:0008296 GO:0008297 GO:0008310 GO:0008408 GO:0008622 GO:0009058 GO:0009059 GO:0009987 GO:0016020 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016895 GO:0018130 GO:0019438 GO:0022402 GO:0022616 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045004 GO:0045005 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0071944 GO:0090304 GO:0090305 GO:0097159 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1903047 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map05166
Pfam Domains
Protein Families
DNA_pol_B DNA_pol_B_exo1 DUF1744

Protein Analysis

189

Amino Acids

20.59

Weight (kDa)

8.84

Isoelectric Point (pI)

31.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 33 - 157 1.3e-11 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 494
AfiI CCNNNNNNNGG 1 cut(s) 421
AgsI TTSAA 4 cut(s) 205, 272, 283, 470
AluBI AGCT 3 cut(s) 263, 278, 478
AluI AGCT 3 cut(s) 263, 278, 478
Alw26I GTCTC 1 cut(s) 96
AoxI GGCC 3 cut(s) 3, 423, 459
ApeKI GCWGC 2 cut(s) 248, 314
AspS9I GGNCC 1 cut(s) 424
AsuHPI GGTGA 1 cut(s) 404
BbvI GCAGC 2 cut(s) 260, 326
BceAI ACGGC 2 cut(s) 206, 327
BcgI CGANNNNNNTGC 2 cut(s) 281, 315
BcoDI GTCTC 1 cut(s) 96
BfaI CTAG 1 cut(s) 565
BfmI CTRYAG 1 cut(s) 139
BglII AGATCT 1 cut(s) 208
BisI GCNGC 2 cut(s) 249, 315
BlsI GCNGC 2 cut(s) 250, 316
BmgT120I GGNCC 1 cut(s) 424
BmrI ACTGGG 1 cut(s) 544
BmuI ACTGGG 1 cut(s) 544
BpmI CTGGAG 1 cut(s) 48
Bsc4I CCNNNNNNNGG 1 cut(s) 421
Bse1I ACTGG 1 cut(s) 550
Bse3DI GCAATG 1 cut(s) 30
BseGI GGATG 1 cut(s) 532
BseLI CCNNNNNNNGG 1 cut(s) 421
BseMI GCAATG 1 cut(s) 30
BseMII CTCAG 1 cut(s) 24
BseNI ACTGG 1 cut(s) 550
BseXI GCAGC 2 cut(s) 260, 326
BsgI GTGCAG 1 cut(s) 267
BshFI GGCC 3 cut(s) 5, 425, 461
BslI CCNNNNNNNGG 1 cut(s) 421
BsmAI GTCTC 1 cut(s) 96
BsmBI CGTCTC 1 cut(s) 96
BsmI GAATGC 2 cut(s) 304, 533
BsnI GGCC 3 cut(s) 5, 425, 461
Bsp143I GATC 2 cut(s) 188, 208
BspANI GGCC 3 cut(s) 5, 425, 461
BspCNI CTCAG 1 cut(s) 23
BsrDI GCAATG 1 cut(s) 30
BsrI ACTGG 1 cut(s) 550
BssMI GATC 2 cut(s) 188, 208
Bst4CI ACNGT 1 cut(s) 392
BstAPI GCANNNNNTGC 1 cut(s) 74
BstC8I GCNNGC 1 cut(s) 116
BstDEI CTNAG 1 cut(s) 10
BstEII GGTNACC 1 cut(s) 403
BstENI CCTNNNNNAGG 1 cut(s) 419
BstF5I GGATG 1 cut(s) 532
BstKTI GATC 2 cut(s) 191, 211
BstMAI GTCTC 1 cut(s) 96
BstMBI GATC 2 cut(s) 188, 208
BstMWI GCNNNNNNNGC 3 cut(s) 74, 248, 284
BstPI GGTNACC 1 cut(s) 403
BstSFI CTRYAG 1 cut(s) 139
BstV1I GCAGC 2 cut(s) 260, 326
BstX2I RGATCY 1 cut(s) 208
BstYI RGATCY 1 cut(s) 208
BsuRI GGCC 3 cut(s) 5, 425, 461
BtsCI GGATG 1 cut(s) 532
BtsI GCAGTG 4 cut(s) 30, 158, 332, 371
BtsIMutI CAGTG 5 cut(s) 30, 158, 332, 371, 495
Cac8I GCNNGC 1 cut(s) 116
Cfr13I GGNCC 1 cut(s) 424
CseI GACGC 1 cut(s) 78
CviAII CATG 1 cut(s) 514
CviJI RGCY 9 cut(s) 5, 138, 221, 263, 278, 317, 425, 461, 478
CviKI_1 RGCY 9 cut(s) 5, 138, 221, 263, 278, 317, 425, 461, 478
DdeI CTNAG 1 cut(s) 10
DpnI GATC 2 cut(s) 190, 210
DpnII GATC 2 cut(s) 188, 208
Eco91I GGTNACC 1 cut(s) 403
EcoNI CCTNNNNNAGG 1 cut(s) 419
EcoO65I GGTNACC 1 cut(s) 403
Esp3I CGTCTC 1 cut(s) 96
FaeI CATG 1 cut(s) 517
FalI AAGNNNNNCTT 4 cut(s) 420, 452, 462, 494
FatI CATG 1 cut(s) 513
FblI GTMKAC 1 cut(s) 494
Fnu4HI GCNGC 2 cut(s) 249, 315
FokI GGATG 1 cut(s) 539
Fsp4HI GCNGC 2 cut(s) 249, 315
FspBI CTAG 1 cut(s) 565
GluI GCNGC 2 cut(s) 249, 315
GsuI CTGGAG 1 cut(s) 48
HaeIII GGCC 3 cut(s) 5, 425, 461
HgaI GACGC 1 cut(s) 78
Hin1II CATG 1 cut(s) 517
HincII GTYRAC 1 cut(s) 495
HindII GTYRAC 1 cut(s) 495
HindIII AAGCTT 1 cut(s) 276
HinfI GANTC 1 cut(s) 335
HphI GGTGA 1 cut(s) 404
Hpy166II GTNNAC 1 cut(s) 495
Hpy188III TCNNGA 2 cut(s) 93, 212
Hpy8I GTNNAC 1 cut(s) 495
Hpy99I CGWCG 2 cut(s) 499, 502
HpyCH4III ACNGT 1 cut(s) 392
HpyCH4IV ACGT 1 cut(s) 266
HpyCH4V TGCA 7 cut(s) 23, 77, 83, 114, 248, 376, 533
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 248, 284
HpyF3I CTNAG 1 cut(s) 10
HpySE526I ACGT 1 cut(s) 266
Hsp92II CATG 1 cut(s) 517
Kzo9I GATC 2 cut(s) 188, 208
LmnI GCTCC 1 cut(s) 239
LpnPI CCDG 4 cut(s) 78, 222, 407, 563
Lsp1109I GCAGC 2 cut(s) 260, 326
MaeI CTAG 1 cut(s) 565
MaeII ACGT 1 cut(s) 266
MaeIII GTNAC 3 cut(s) 392, 403, 541
MalI GATC 2 cut(s) 190, 210
MboI GATC 2 cut(s) 188, 208
MflI RGATCY 1 cut(s) 208
MluCI AATT 4 cut(s) 131, 183, 199, 230
MnlI CCTC 5 cut(s) 16, 19, 62, 425, 472
MseI TTAA 2 cut(s) 228, 557
Mva1269I GAATGC 2 cut(s) 304, 533
MwoI GCNNNNNNNGC 3 cut(s) 74, 248, 284
NdeII GATC 2 cut(s) 188, 208
NlaIII CATG 1 cut(s) 517
NmuCI GTSAC 1 cut(s) 392
PctI GAATGC 2 cut(s) 304, 533
PfeI GAWTC 1 cut(s) 335
PkrI GCNGC 2 cut(s) 250, 316
PspEI GGTNACC 1 cut(s) 403
PspPI GGNCC 1 cut(s) 424
PsuI RGATCY 1 cut(s) 208
SalI GTCGAC 1 cut(s) 493
SaqAI TTAA 2 cut(s) 228, 557
SatI GCNGC 2 cut(s) 249, 315
Sau3AI GATC 2 cut(s) 188, 208
Sau96I GGNCC 1 cut(s) 424
SetI ASST 6 cut(s) 146, 265, 269, 280, 438, 480
SfcI CTRYAG 1 cut(s) 139
Sse9I AATT 4 cut(s) 131, 183, 199, 230
SspMI CTAG 1 cut(s) 565
TaaI ACNGT 1 cut(s) 392
TaiI ACGT 1 cut(s) 269
TaqI TCGA 2 cut(s) 94, 494
TasI AATT 4 cut(s) 131, 183, 199, 230
TfiI GAWTC 1 cut(s) 335
Tru1I TTAA 2 cut(s) 228, 557
Tru9I TTAA 2 cut(s) 228, 557
TscAI CASTG 5 cut(s) 30, 158, 332, 378, 495
TseFI GTSAC 1 cut(s) 392
TseI GCWGC 2 cut(s) 248, 314
Tsp45I GTSAC 1 cut(s) 392
TspDTI ATGAA 1 cut(s) 19
TspRI CASTG 5 cut(s) 30, 158, 332, 378, 495
XagI CCTNNNNNAGG 1 cut(s) 419
XmiI GTMKAC 1 cut(s) 494
XspI CTAG 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.