pycom10g02080

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0005336
Physical Location & Seq
Reverse (-)
2219545 .. 2220078
534 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g02080.1

Sequence Viewer

Length: 534 bp
ATGCATCAAATGGAGTCCCCAATTCACTATGTGTTACCATTCTTTTTTACCTCTTCGTTCTTGTTTCTCTCTTCACCTTCACCTATGAATGCATCAATTCATCTGGTTGAAACTGTTTGCAAAAAGACCATCAGTAACTCTGAATGCCTAAAAGCTTTCGACTCCGATCCTCGATCTAGTTCAGCATCGAATTACAAGGAGCTTGCTATAGTAGCTCTTGAGTTAGCTATAGCGAATGCAAAAGATAGCCAAAACTTCATGAACAATTTGCTCAAGAGTGATCCCAGGAAGCCAATTAAGCTGTGTTCTTATTTTTACGGCGGTGTGGTTGGTTCATTCAATAGCGCCAAAAAAGAGATAGATGAGGATCCTTTGACGGCCAACTATGATGTCAAAATTGCAGGCGATGCTGCAGGTTACTGTGAAACTGCCTTGAGTTCTGAAGGGGTGGAAGTATCTAAAATATCTACTAGAAACCATGTTGTCAAATTATACAGTAGTATTGGATATGTTATCACCGACCAACTTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

178

Amino Acids

19.38

Weight (kDa)

5.78

Isoelectric Point (pI)

45.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 35 - 166 2.4e-14 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 404
AciI CCGC 1 cut(s) 321
AclWI GGATC 4 cut(s) 161, 275, 362, 375
AcoI YGGCCR 1 cut(s) 378
AcuI CTGAAG 1 cut(s) 462
AdeI CACNNNGTG 1 cut(s) 31
AgsI TTSAA 2 cut(s) 110, 340
AjnI CCWGG 1 cut(s) 284
AluBI AGCT 5 cut(s) 155, 202, 215, 227, 301
AluI AGCT 5 cut(s) 155, 202, 215, 227, 301
AlwI GGATC 4 cut(s) 161, 275, 362, 375
AoxI GGCC 1 cut(s) 378
ApeKI GCWGC 1 cut(s) 410
AspLEI GCGC 1 cut(s) 347
AsuHPI GGTGA 3 cut(s) 66, 72, 508
BamHI GGATCC 1 cut(s) 367
BbvI GCAGC 1 cut(s) 397
BccI CCATC 1 cut(s) 137
BceAI ACGGC 2 cut(s) 334, 393
BcgI CGANNNNNNTGC 1 cut(s) 509
BciT130I CCWGG 1 cut(s) 286
BfaI CTAG 2 cut(s) 177, 471
BfmI CTRYAG 3 cut(s) 207, 228, 411
BfoI RGCGCY 1 cut(s) 348
BfuAI ACCTGC 1 cut(s) 404
BisI GCNGC 1 cut(s) 411
BlsI GCNGC 1 cut(s) 412
Bme1390I CCNGG 1 cut(s) 286
BmiI GGNNCC 1 cut(s) 369
BmrFI CCNGG 1 cut(s) 286
BmsI GCATC 4 cut(s) 13, 101, 194, 397
BpuEI CTTGAG 3 cut(s) 239, 257, 454
BsaBI GATNNNNATC 1 cut(s) 366
BsaJI CCNNGG 1 cut(s) 284
Bse8I GATNNNNATC 1 cut(s) 366
BseBI CCWGG 1 cut(s) 286
BseDI CCNNGG 1 cut(s) 284
BseJI GATNNNNATC 1 cut(s) 366
BseXI GCAGC 1 cut(s) 397
BshFI GGCC 1 cut(s) 380
BsmI GAATGC 3 cut(s) 94, 149, 241
BsnI GGCC 1 cut(s) 380
Bsp143I GATC 4 cut(s) 166, 173, 280, 367
BspACI CCGC 1 cut(s) 321
BspANI GGCC 1 cut(s) 380
BspHI TCATGA 1 cut(s) 258
BspLI GGNNCC 1 cut(s) 369
BspMAI CTGCAG 1 cut(s) 415
BspMI ACCTGC 1 cut(s) 404
BspPI GGATC 4 cut(s) 161, 275, 362, 375
BssECI CCNNGG 1 cut(s) 284
BssMI GATC 4 cut(s) 166, 173, 280, 367
Bst2UI CCWGG 1 cut(s) 286
Bst4CI ACNGT 3 cut(s) 115, 422, 497
Bst6I CTCTTC 2 cut(s) 58, 76
BstAPI GCANNNNNTGC 1 cut(s) 407
BstC8I GCNNGC 2 cut(s) 204, 403
BstH2I RGCGCY 1 cut(s) 348
BstHHI GCGC 1 cut(s) 347
BstKTI GATC 4 cut(s) 169, 176, 283, 370
BstMBI GATC 4 cut(s) 166, 173, 280, 367
BstMWI GCNNNNNNNGC 3 cut(s) 212, 298, 407
BstNI CCWGG 1 cut(s) 286
BstSCI CCNGG 1 cut(s) 284
BstSFI CTRYAG 3 cut(s) 207, 228, 411
BstV1I GCAGC 1 cut(s) 397
BstX2I RGATCY 1 cut(s) 367
BstYI RGATCY 1 cut(s) 367
BsuRI GGCC 1 cut(s) 380
BtgZI GCGATG 1 cut(s) 420
BveI ACCTGC 1 cut(s) 404
Cac8I GCNNGC 2 cut(s) 204, 403
CciI TCATGA 1 cut(s) 258
CfoI GCGC 1 cut(s) 347
CviAII CATG 2 cut(s) 259, 479
CviJI RGCY 8 cut(s) 155, 202, 215, 227, 249, 292, 301, 380
CviKI_1 RGCY 8 cut(s) 155, 202, 215, 227, 249, 292, 301, 380
DpnI GATC 4 cut(s) 168, 175, 282, 369
DpnII GATC 4 cut(s) 166, 173, 280, 367
DraIII CACNNNGTG 1 cut(s) 31
EaeI YGGCCR 1 cut(s) 378
Eam1104I CTCTTC 2 cut(s) 58, 76
EarI CTCTTC 2 cut(s) 58, 76
Eco57I CTGAAG 1 cut(s) 462
EcoRII CCWGG 1 cut(s) 284
EcoT22I ATGCAT 2 cut(s) 6, 94
FaeI CATG 2 cut(s) 262, 482
FaiI YATR 9 cut(s) 30, 86, 209, 230, 260, 387, 480, 493, 510
FatI CATG 2 cut(s) 258, 478
Fnu4HI GCNGC 1 cut(s) 411
Fsp4HI GCNGC 1 cut(s) 411
FspBI CTAG 2 cut(s) 177, 471
GlaI GCGC 1 cut(s) 346
GluI GCNGC 1 cut(s) 411
HaeII RGCGCY 1 cut(s) 348
HaeIII GGCC 1 cut(s) 380
HhaI GCGC 1 cut(s) 347
Hin1II CATG 2 cut(s) 262, 482
Hin6I GCGC 1 cut(s) 345
HinP1I GCGC 1 cut(s) 345
HindIII AAGCTT 1 cut(s) 153
HinfI GANTC 2 cut(s) 14, 161
HphI GGTGA 3 cut(s) 66, 72, 508
Hpy188I TCNGA 3 cut(s) 142, 166, 442
Hpy188III TCNNGA 3 cut(s) 218, 259, 274
HpyAV CCTTC 2 cut(s) 87, 437
HpyCH4III ACNGT 3 cut(s) 115, 422, 497
HpyCH4V TGCA 6 cut(s) 4, 92, 120, 239, 401, 413
HpyF10VI GCNNNNNNNGC 3 cut(s) 212, 298, 407
Hsp92II CATG 2 cut(s) 262, 482
HspAI GCGC 1 cut(s) 345
Kzo9I GATC 4 cut(s) 166, 173, 280, 367
LmnI GCTCC 1 cut(s) 199
LpnPI CCDG 5 cut(s) 89, 271, 298, 387, 399
Lsp1109I GCAGC 1 cut(s) 397
LweI GCATC 4 cut(s) 13, 101, 194, 397
MaeI CTAG 2 cut(s) 177, 471
MaeIII GTNAC 3 cut(s) 33, 134, 416
MalI GATC 4 cut(s) 168, 175, 282, 369
MboI GATC 4 cut(s) 166, 173, 280, 367
MboII GAAGA 2 cut(s) 45, 63
MflI RGATCY 1 cut(s) 367
MluCI AATT 7 cut(s) 21, 96, 190, 265, 294, 396, 488
MlyI GAGTC 2 cut(s) 23, 155
MnlI CCTC 3 cut(s) 61, 180, 358
Mph1103I ATGCAT 2 cut(s) 6, 94
MseI TTAA 1 cut(s) 297
MspR9I CCNGG 1 cut(s) 286
Mva1269I GAATGC 3 cut(s) 94, 149, 241
MvaI CCWGG 1 cut(s) 286
MwoI GCNNNNNNNGC 3 cut(s) 212, 298, 407
NdeII GATC 4 cut(s) 166, 173, 280, 367
NlaIII CATG 2 cut(s) 262, 482
NlaIV GGNNCC 1 cut(s) 369
NsiI ATGCAT 2 cut(s) 6, 94
PagI TCATGA 1 cut(s) 258
PctI GAATGC 3 cut(s) 94, 149, 241
PkrI GCNGC 1 cut(s) 412
PleI GAGTC 2 cut(s) 22, 155
PpsI GAGTC 2 cut(s) 22, 155
Psp6I CCWGG 1 cut(s) 284
PspGI CCWGG 1 cut(s) 284
PspN4I GGNNCC 1 cut(s) 369
PstI CTGCAG 1 cut(s) 415
PsuI RGATCY 1 cut(s) 367
SaqAI TTAA 1 cut(s) 297
SatI GCNGC 1 cut(s) 411
Sau3AI GATC 4 cut(s) 166, 173, 280, 367
SchI GAGTC 2 cut(s) 23, 155
ScrFI CCNGG 1 cut(s) 286
SetI ASST 9 cut(s) 53, 79, 85, 157, 204, 217, 229, 303, 418
SfaNI GCATC 4 cut(s) 13, 101, 194, 397
SfcI CTRYAG 3 cut(s) 207, 228, 411
SmlI CTYRAG 3 cut(s) 218, 272, 433
SmoI CTYRAG 3 cut(s) 218, 272, 433
Sse9I AATT 7 cut(s) 21, 96, 190, 265, 294, 396, 488
SsiI CCGC 1 cut(s) 321
SspMI CTAG 2 cut(s) 177, 471
StyD4I CCNGG 1 cut(s) 284
TaaI ACNGT 3 cut(s) 115, 422, 497
TaqI TCGA 3 cut(s) 159, 172, 188
TasI AATT 7 cut(s) 21, 96, 190, 265, 294, 396, 488
Tru1I TTAA 1 cut(s) 297
Tru9I TTAA 1 cut(s) 297
TseI GCWGC 1 cut(s) 410
TspDTI ATGAA 5 cut(s) 89, 101, 247, 275, 324
XspI CTAG 2 cut(s) 177, 471
Zsp2I ATGCAT 2 cut(s) 6, 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.