RLG00000013535

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0005336
Physical Location & Seq
Reverse (-)
32810530 .. 32811072
543 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013535

Sequence Viewer

Length: 543 bp
ATGGAGTCTCCACTAGGTTTTGCGTCAACCTTGTTCTTTGCTTCCCTGTTCTTACTTCTCTCTACCCCTTCACCCACAAATGCATCAACTCGTTTAGTTGAATCTGTGTGCAATGAAGTAGAAGGCATTTTGAAAGGCAGCAACTCTGAATGCTTGAAAGCTCTTGACTTGGATCCTCGAACCGGATCGGCGTCCACTTACGAAGTGCTTGCCCCAATCGCTATCGATTCAGCAATAGCAAATGCAAGGGATAGTGAAGCTTTCATTAAAAATTTGCTCAAGAACAACCCTAGCAGTGAAGCTATAAAACAGTGTGCAGATTCTTATAATATAGTGGTAAGGGATTTTGTAGGTGCCAAAGAGGAGCTGAAGGAGGACCCTTTGTCTGCCAACTATGACATCAAAATTGCCGGCGACAACGTCCATGATTGTGAGACCGCATTGAGTTCTAAAGGGCTTCAAGTTCCTGAAATAGCTTCCAGAAACCATGCAATGTTCTTATATAGTAATATTGGATTTGTTATCACTAATCATATTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

181

Amino Acids

19.39

Weight (kDa)

4.7

Isoelectric Point (pI)

41.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 32 - 160 2.3e-10 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 327
AccB1I GGYRCC 1 cut(s) 353
AciI CCGC 1 cut(s) 438
AclWI GGATC 3 cut(s) 167, 180, 193
AcsI RAATTY 1 cut(s) 271
AcuI CTGAAG 1 cut(s) 389
AcyI GRCGYC 1 cut(s) 191
AfiI CCNNNNNNNGG 1 cut(s) 182
AgsI TTSAA 4 cut(s) 101, 133, 157, 461
AhdI GACNNNNNGTC 1 cut(s) 382
AluBI AGCT 5 cut(s) 161, 260, 302, 367, 476
AluI AGCT 5 cut(s) 161, 260, 302, 367, 476
Alw26I GTCTC 2 cut(s) 12, 428
AlwI GGATC 3 cut(s) 167, 180, 193
ApeKI GCWGC 1 cut(s) 138
ApoI RAATTY 1 cut(s) 271
AspS9I GGNCC 1 cut(s) 376
AsuHPI GGTGA 1 cut(s) 63
AvaII GGWCC 1 cut(s) 376
BamHI GGATCC 1 cut(s) 172
BanI GGYRCC 1 cut(s) 353
BbvI GCAGC 1 cut(s) 150
BcgI CGANNNNNNTGC 2 cut(s) 191, 225
BcoDI GTCTC 2 cut(s) 12, 428
BfaI CTAG 2 cut(s) 14, 291
BisI GCNGC 1 cut(s) 139
BlsI GCNGC 1 cut(s) 140
Bme18I GGWCC 1 cut(s) 376
BmeRI GACNNNNNGTC 1 cut(s) 382
BmgT120I GGNCC 1 cut(s) 376
BmiI GGNNCC 3 cut(s) 174, 355, 378
BmsI GCATC 1 cut(s) 92
BpuEI CTTGAG 1 cut(s) 263
Bsa29I ATCGAT 1 cut(s) 225
BsaHI GRCGYC 1 cut(s) 191
BsaI GGTCTC 1 cut(s) 428
BsaWI WCCGGW 1 cut(s) 182
Bsc4I CCNNNNNNNGG 1 cut(s) 182
Bse118I RCCGGY 1 cut(s) 410
Bse3DI GCAATG 2 cut(s) 118, 498
BseCI ATCGAT 1 cut(s) 225
BseLI CCNNNNNNNGG 1 cut(s) 182
BseMI GCAATG 2 cut(s) 118, 498
BseRI GAGGAG 1 cut(s) 377
BseXI GCAGC 1 cut(s) 150
BsgI GTGCAG 1 cut(s) 336
BshNI GGYRCC 1 cut(s) 353
BshVI ATCGAT 1 cut(s) 225
BsiSI CCGG 2 cut(s) 183, 411
BslI CCNNNNNNNGG 1 cut(s) 182
BsmAI GTCTC 2 cut(s) 12, 428
BsmI GAATGC 1 cut(s) 155
Bso31I GGTCTC 1 cut(s) 428
Bsp143I GATC 2 cut(s) 172, 185
BspACI CCGC 1 cut(s) 438
BspDI ATCGAT 1 cut(s) 225
BspLI GGNNCC 3 cut(s) 174, 355, 378
BspPI GGATC 3 cut(s) 167, 180, 193
BspT107I GGYRCC 1 cut(s) 353
BspTNI GGTCTC 1 cut(s) 428
BsrDI GCAATG 2 cut(s) 118, 498
BsrFI RCCGGY 1 cut(s) 410
BssAI RCCGGY 1 cut(s) 410
BssMI GATC 2 cut(s) 172, 185
BssNI GRCGYC 1 cut(s) 191
Bst4CI ACNGT 1 cut(s) 312
BstACI GRCGYC 1 cut(s) 191
BstC8I GCNNGC 2 cut(s) 210, 412
BstKTI GATC 2 cut(s) 175, 188
BstMAI GTCTC 2 cut(s) 12, 428
BstMBI GATC 2 cut(s) 172, 185
BstMWI GCNNNNNNNGC 1 cut(s) 218
BstV1I GCAGC 1 cut(s) 150
BstX2I RGATCY 1 cut(s) 172
BstYI RGATCY 1 cut(s) 172
Bsu15I ATCGAT 1 cut(s) 225
BsuTUI ATCGAT 1 cut(s) 225
BtsI GCAGTG 1 cut(s) 301
BtsIMutI CAGTG 2 cut(s) 301, 317
Cac8I GCNNGC 2 cut(s) 210, 412
Cfr10I RCCGGY 1 cut(s) 410
Cfr13I GGNCC 1 cut(s) 376
ClaI ATCGAT 1 cut(s) 225
CseI GACGC 2 cut(s) 12, 180
CviAII CATG 2 cut(s) 425, 488
CviJI RGCY 6 cut(s) 161, 260, 302, 367, 457, 476
CviKI_1 RGCY 6 cut(s) 161, 260, 302, 367, 457, 476
DpnI GATC 2 cut(s) 174, 187
DpnII GATC 2 cut(s) 172, 185
DriI GACNNNNNGTC 1 cut(s) 382
Eam1105I GACNNNNNGTC 1 cut(s) 382
Eco31I GGTCTC 1 cut(s) 428
Eco47I GGWCC 1 cut(s) 376
Eco57I CTGAAG 1 cut(s) 389
EcoO109I RGGNCCY 1 cut(s) 376
EcoT22I ATGCAT 1 cut(s) 85
FaeI CATG 2 cut(s) 428, 491
FaiI YATR 9 cut(s) 305, 327, 332, 396, 426, 489, 502, 504, 534
FatI CATG 2 cut(s) 424, 487
Fnu4HI GCNGC 1 cut(s) 139
Fsp4HI GCNGC 1 cut(s) 139
FspBI CTAG 2 cut(s) 14, 291
GluI GCNGC 1 cut(s) 139
HapII CCGG 2 cut(s) 183, 411
HgaI GACGC 2 cut(s) 12, 180
Hin1I GRCGYC 1 cut(s) 191
Hin1II CATG 2 cut(s) 428, 491
HincII GTYRAC 1 cut(s) 27
HindII GTYRAC 1 cut(s) 27
HindIII AAGCTT 1 cut(s) 258
HinfI GANTC 4 cut(s) 5, 101, 227, 320
HpaII CCGG 2 cut(s) 183, 411
HphI GGTGA 1 cut(s) 63
Hpy166II GTNNAC 2 cut(s) 27, 195
Hpy188I TCNGA 1 cut(s) 148
Hpy188III TCNNGA 4 cut(s) 164, 280, 467, 480
Hpy8I GTNNAC 2 cut(s) 27, 195
HpyAV CCTTC 3 cut(s) 78, 116, 364
HpyCH4III ACNGT 1 cut(s) 312
HpyCH4IV ACGT 1 cut(s) 420
HpyCH4V TGCA 5 cut(s) 83, 111, 245, 317, 491
HpyF10VI GCNNNNNNNGC 1 cut(s) 218
HpySE526I ACGT 1 cut(s) 420
Hsp92I GRCGYC 1 cut(s) 191
Hsp92II CATG 2 cut(s) 428, 491
KroI GCCGGC 1 cut(s) 410
KroNI GCCGGC 1 cut(s) 412
Kzo9I GATC 2 cut(s) 172, 185
LmnI GCTCC 1 cut(s) 364
LpnPI CCDG 5 cut(s) 59, 196, 424, 480, 493
Lsp1109I GCAGC 1 cut(s) 150
LweI GCATC 1 cut(s) 92
MaeI CTAG 2 cut(s) 14, 291
MaeII ACGT 1 cut(s) 420
MalI GATC 2 cut(s) 174, 187
MboI GATC 2 cut(s) 172, 185
MflI RGATCY 1 cut(s) 172
MluCI AATT 2 cut(s) 271, 405
MlyI GAGTC 1 cut(s) 14
MnlI CCTC 3 cut(s) 186, 355, 367
Mph1103I ATGCAT 1 cut(s) 85
MroNI GCCGGC 1 cut(s) 410
MseI TTAA 2 cut(s) 267, 541
MslI CAYNNNNRTG 1 cut(s) 429
MspI CCGG 2 cut(s) 183, 411
Mva1269I GAATGC 1 cut(s) 155
MwoI GCNNNNNNNGC 1 cut(s) 218
NaeI GCCGGC 1 cut(s) 412
NdeII GATC 2 cut(s) 172, 185
NgoMIV GCCGGC 1 cut(s) 410
NlaIII CATG 2 cut(s) 428, 491
NlaIV GGNNCC 3 cut(s) 174, 355, 378
NsiI ATGCAT 1 cut(s) 85
PctI GAATGC 1 cut(s) 155
PdiI GCCGGC 1 cut(s) 412
PfeI GAWTC 3 cut(s) 101, 227, 320
PflFI GACNNNGTC 1 cut(s) 419
PkrI GCNGC 1 cut(s) 140
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
PpuMI RGGWCCY 1 cut(s) 376
PsiI TTATAA 1 cut(s) 327
Psp5II RGGWCCY 1 cut(s) 376
PspN4I GGNNCC 3 cut(s) 174, 355, 378
PspPI GGNCC 1 cut(s) 376
PspPPI RGGWCCY 1 cut(s) 376
PsuI RGATCY 1 cut(s) 172
PsyI GACNNNGTC 1 cut(s) 419
RseI CAYNNNNRTG 1 cut(s) 429
SaqAI TTAA 2 cut(s) 267, 541
SatI GCNGC 1 cut(s) 139
Sau3AI GATC 2 cut(s) 172, 185
Sau96I GGNCC 1 cut(s) 376
SchI GAGTC 1 cut(s) 14
SetI ASST 9 cut(s) 19, 32, 163, 262, 304, 355, 369, 423, 478
SfaNI GCATC 1 cut(s) 92
SinI GGWCC 1 cut(s) 376
SmiMI CAYNNNNRTG 1 cut(s) 429
SmlI CTYRAG 1 cut(s) 278
SmoI CTYRAG 1 cut(s) 278
Sse9I AATT 2 cut(s) 271, 405
SsiI CCGC 1 cut(s) 438
SspI AATATT 1 cut(s) 511
SspMI CTAG 2 cut(s) 14, 291
TaaI ACNGT 1 cut(s) 312
TaiI ACGT 1 cut(s) 423
TaqI TCGA 2 cut(s) 178, 225
TasI AATT 2 cut(s) 271, 405
TfiI GAWTC 3 cut(s) 101, 227, 320
Tru1I TTAA 2 cut(s) 267, 541
Tru9I TTAA 2 cut(s) 267, 541
TscAI CASTG 2 cut(s) 301, 317
TseI GCWGC 1 cut(s) 138
TspDTI ATGAA 2 cut(s) 129, 253
TspRI CASTG 2 cut(s) 301, 317
Tth111I GACNNNGTC 1 cut(s) 419
VpaK11BI GGWCC 1 cut(s) 376
XapI RAATTY 1 cut(s) 271
XspI CTAG 2 cut(s) 14, 291
Zsp2I ATGCAT 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.