AT5G50070

Invertase pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005336
Physical Location & Seq
Forward (+)
20364516 .. 20365481
966 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50070.1

Sequence Viewer

Length: 552 bp
ATGACTTCTTCTTCTCATTGTTTCTTGATCGTTTCAGTGGCACTTCTATTGCAAGTTCTCTTATCCCCTGTTTCGGCCTTAACCTTAACCTCCACAAAATACATTAATAAATTATGCCAGATGTCATTTGTCGACAATAAACCCTTCTGTTTGCAAACATTGAGTGCCTATCCTCCTGCGGCTTCTGCCACTGGCTTGCTCCCACTTGCGTCAGCCGTGATCCGCGGCATTGTCCTACCCCAAGCCAAGAAGTCTGCGAGTTTTGCAAATGGAGCAGCGAAAAAGGAGCCGAGCTTGAAGACACAGTTCCAGACGTGCCAAGAGGCCTTTGTAAGTATTGGAGATTCTCTCATGAGCGCTTTTGAAGAACTCAAGCGCGATCCACATATCGCAACCTATGATGTTATGACTTGTACCGACCATACAACCCAAGTGAAGAATTTGGTCGGAAAAAACACAGATGTGGCTTCGAAGACTCTCATAGAGATGACAATGCAAATGGAGAAGCTCATTCCTCTTGCTCTTGCATCCACACAAGTTCTTGGCGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

183

Amino Acids

19.59

Weight (kDa)

8.6

Isoelectric Point (pI)

37.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 34 - 175 2.9e-10 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 132
AccII CGCG 2 cut(s) 225, 378
AciI CCGC 4 cut(s) 179, 223, 225, 546
AclWI GGATC 2 cut(s) 214, 374
AcsI RAATTY 1 cut(s) 439
AfaI GTAC 1 cut(s) 415
AfeI AGCGCT 1 cut(s) 358
AfiI CCNNNNNNNGG 1 cut(s) 73
AgsI TTSAA 2 cut(s) 298, 365
AjiI CACGTC 1 cut(s) 315
AleI CACNNNNGTG 1 cut(s) 461
AluBI AGCT 2 cut(s) 294, 508
AluI AGCT 2 cut(s) 294, 508
AlwI GGATC 2 cut(s) 214, 374
Aor51HI AGCGCT 1 cut(s) 358
AoxI GGCC 2 cut(s) 75, 324
ApeKI GCWGC 1 cut(s) 275
ApoI RAATTY 1 cut(s) 439
AseI ATTAAT 1 cut(s) 105
AspLEI GCGC 2 cut(s) 359, 378
AsuII TTCGAA 1 cut(s) 470
BbsI GAAGAC 2 cut(s) 305, 479
BbvI GCAGC 1 cut(s) 287
BceAI ACGGC 1 cut(s) 200
BfoI RGCGCY 1 cut(s) 360
BisI GCNGC 3 cut(s) 180, 226, 276
BlsI GCNGC 3 cut(s) 181, 227, 277
BmgBI CACGTC 1 cut(s) 315
BmiI GGNNCC 1 cut(s) 288
BmsI GCATC 1 cut(s) 536
BpiI GAAGAC 2 cut(s) 305, 479
BplI GAGNNNNNCTC 2 cut(s) 333, 365
Bpu14I TTCGAA 1 cut(s) 470
BpuEI CTTGAG 1 cut(s) 356
BsaJI CCNNGG 1 cut(s) 223
Bsc4I CCNNNNNNNGG 1 cut(s) 73
Bse1I ACTGG 1 cut(s) 196
BseDI CCNNGG 1 cut(s) 223
BseGI GGATG 1 cut(s) 527
BseLI CCNNNNNNNGG 1 cut(s) 73
BseNI ACTGG 1 cut(s) 196
BseXI GCAGC 1 cut(s) 287
Bsh1236I CGCG 2 cut(s) 225, 378
BshFI GGCC 2 cut(s) 77, 326
BslI CCNNNNNNNGG 1 cut(s) 73
BsnI GGCC 2 cut(s) 77, 326
Bsp119I TTCGAA 1 cut(s) 470
Bsp143I GATC 3 cut(s) 27, 219, 379
BspACI CCGC 4 cut(s) 179, 223, 225, 546
BspANI GGCC 2 cut(s) 77, 326
BspFNI CGCG 2 cut(s) 225, 378
BspHI TCATGA 1 cut(s) 351
BspLI GGNNCC 1 cut(s) 288
BspPI GGATC 2 cut(s) 214, 374
BspT104I TTCGAA 1 cut(s) 470
BsrI ACTGG 1 cut(s) 196
BssECI CCNNGG 1 cut(s) 223
BssMI GATC 3 cut(s) 27, 219, 379
Bst4CI ACNGT 1 cut(s) 306
BstBI TTCGAA 1 cut(s) 470
BstC8I GCNNGC 1 cut(s) 197
BstDSI CCRYGG 1 cut(s) 223
BstF5I GGATG 1 cut(s) 527
BstFNI CGCG 2 cut(s) 225, 378
BstH2I RGCGCY 1 cut(s) 360
BstHHI GCGC 2 cut(s) 359, 378
BstKTI GATC 3 cut(s) 30, 222, 382
BstMBI GATC 3 cut(s) 27, 219, 379
BstMWI GCNNNNNNNGC 3 cut(s) 185, 263, 272
BstUI CGCG 2 cut(s) 225, 378
BstV1I GCAGC 1 cut(s) 287
BstV2I GAAGAC 2 cut(s) 305, 479
BsuRI GGCC 2 cut(s) 77, 326
BtgI CCRYGG 1 cut(s) 223
BtrI CACGTC 1 cut(s) 315
BtsCI GGATG 1 cut(s) 527
BtsIMutI CAGTG 2 cut(s) 42, 189
Cac8I GCNNGC 1 cut(s) 197
CciI TCATGA 1 cut(s) 351
CfoI GCGC 2 cut(s) 359, 378
Cfr42I CCGCGG 1 cut(s) 226
CseI GACGC 1 cut(s) 198
Csp6I GTAC 1 cut(s) 414
CviAII CATG 1 cut(s) 352
CviQI GTAC 1 cut(s) 414
DpnI GATC 3 cut(s) 29, 221, 381
DpnII GATC 3 cut(s) 27, 219, 379
Eco147I AGGCCT 1 cut(s) 326
Eco47III AGCGCT 1 cut(s) 358
FaeI CATG 1 cut(s) 355
FaiI YATR 7 cut(s) 115, 353, 387, 399, 407, 423, 482
FatI CATG 1 cut(s) 351
FauI CCCGC 1 cut(s) 539
FblI GTMKAC 1 cut(s) 132
Fnu4HI GCNGC 3 cut(s) 180, 226, 276
FokI GGATG 1 cut(s) 514
Fsp4HI GCNGC 3 cut(s) 180, 226, 276
GlaI GCGC 2 cut(s) 358, 377
GluI GCNGC 3 cut(s) 180, 226, 276
HaeII RGCGCY 1 cut(s) 360
HaeIII GGCC 2 cut(s) 77, 326
HgaI GACGC 1 cut(s) 198
HhaI GCGC 2 cut(s) 359, 378
Hin1II CATG 1 cut(s) 355
Hin6I GCGC 2 cut(s) 357, 376
HinP1I GCGC 2 cut(s) 357, 376
HincII GTYRAC 1 cut(s) 133
HindII GTYRAC 1 cut(s) 133
HinfI GANTC 2 cut(s) 344, 475
Hpy166II GTNNAC 1 cut(s) 133
Hpy188I TCNGA 1 cut(s) 449
Hpy188III TCNNGA 3 cut(s) 25, 310, 352
Hpy8I GTNNAC 1 cut(s) 133
HpyAV CCTTC 1 cut(s) 154
HpyCH4III ACNGT 1 cut(s) 306
HpyCH4IV ACGT 1 cut(s) 314
HpyCH4V TGCA 5 cut(s) 52, 154, 266, 496, 527
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 263, 272
HpySE526I ACGT 1 cut(s) 314
Hsp92II CATG 1 cut(s) 355
HspAI GCGC 2 cut(s) 357, 376
KspI CCGCGG 1 cut(s) 226
Kzo9I GATC 3 cut(s) 27, 219, 379
LmnI GCTCC 3 cut(s) 204, 272, 286
LpnPI CCDG 5 cut(s) 81, 131, 177, 189, 323
Lsp1109I GCAGC 1 cut(s) 287
LweI GCATC 1 cut(s) 536
MaeII ACGT 1 cut(s) 314
MalI GATC 3 cut(s) 29, 221, 381
MboI GATC 3 cut(s) 27, 219, 379
MboII GAAGA 5 cut(s) 3, 310, 377, 448, 484
MluCI AATT 2 cut(s) 110, 439
MlyI GAGTC 1 cut(s) 469
MmeI TCCRAC 1 cut(s) 427
MnlI CCTC 4 cut(s) 100, 183, 316, 525
MseI TTAA 3 cut(s) 80, 86, 105
MslI CAYNNNNRTG 2 cut(s) 461, 485
MspA1I CMGCKG 1 cut(s) 225
MvnI CGCG 2 cut(s) 225, 378
MwoI GCNNNNNNNGC 3 cut(s) 185, 263, 272
NdeII GATC 3 cut(s) 27, 219, 379
NlaIII CATG 1 cut(s) 355
NlaIV GGNNCC 1 cut(s) 288
NmeAIII GCCGAG 1 cut(s) 315
NspV TTCGAA 1 cut(s) 470
OliI CACNNNNGTG 1 cut(s) 461
PagI TCATGA 1 cut(s) 351
PceI AGGCCT 1 cut(s) 326
PfeI GAWTC 1 cut(s) 344
PkrI GCNGC 3 cut(s) 181, 227, 277
PleI GAGTC 1 cut(s) 469
PpsI GAGTC 1 cut(s) 469
PshBI ATTAAT 1 cut(s) 105
PspN4I GGNNCC 1 cut(s) 288
RsaI GTAC 1 cut(s) 415
RsaNI GTAC 1 cut(s) 414
RseI CAYNNNNRTG 2 cut(s) 461, 485
SacII CCGCGG 1 cut(s) 226
SalI GTCGAC 1 cut(s) 131
SaqAI TTAA 3 cut(s) 80, 86, 105
SatI GCNGC 3 cut(s) 180, 226, 276
Sau3AI GATC 3 cut(s) 27, 219, 379
SchI GAGTC 1 cut(s) 469
SetI ASST 6 cut(s) 86, 92, 296, 317, 398, 510
SfaNI GCATC 1 cut(s) 536
Sfr303I CCGCGG 1 cut(s) 226
SfuI TTCGAA 1 cut(s) 470
SgrBI CCGCGG 1 cut(s) 226
SmiMI CAYNNNNRTG 2 cut(s) 461, 485
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
Sse9I AATT 2 cut(s) 110, 439
SseBI AGGCCT 1 cut(s) 326
SsiI CCGC 4 cut(s) 179, 223, 225, 546
StuI AGGCCT 1 cut(s) 326
TaaI ACNGT 1 cut(s) 306
TaiI ACGT 1 cut(s) 317
TaqI TCGA 2 cut(s) 132, 470
TasI AATT 2 cut(s) 110, 439
TauI GCSGC 2 cut(s) 182, 228
TfiI GAWTC 1 cut(s) 344
Tru1I TTAA 3 cut(s) 80, 86, 105
Tru9I TTAA 3 cut(s) 80, 86, 105
TscAI CASTG 2 cut(s) 42, 196
TseI GCWGC 1 cut(s) 275
TspRI CASTG 2 cut(s) 42, 196
VspI ATTAAT 1 cut(s) 105
XapI RAATTY 1 cut(s) 439
XmiI GTMKAC 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.