AT5G50060

Invertase pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005336
Physical Location & Seq
Forward (+)
20362486 .. 20363597
1112 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50060.1

Sequence Viewer

Length: 501 bp
ATGGCAGTTCTATCCGAAATTCTCTTTATCTCTTCTTCGGGCTTAACCTCCACAAAATATATTGATGCGCTATGCCAACATGTGGAAAACGGTACCTTCTGCATGCACATGTTGAGAGCCTATCCTCCCGCGGTTACTGCCACCGATTTGTTTTCCCTGGCTAACATCGTGATCAACCTCGGCATATCCTACGCCAACAACACGGGGGCTTTTGCCGCCGAAGCGGCTAAGAAAGAGCCAACGTTGAAGGTGCAGTTCAAGTCATTCCAGCATGAATATGACGGTATTCAGTTGTATCTAAGAATGGCAAGGGGGGAGCTCAAGGAATCTCCAATGAGTGCAAACTATGACATTTTTCGTTGTACGGATATCACTATGTACCTGAAGGATGTGGTCGGAGGAAACAGAGACAACACCTCTAAAACGTTCATGGATATGACATTGCAAATGGAGAACATTATCGATGTTGCCCTTGGAGCCACCGTAGCTCTTGGCGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

166

Amino Acids

18.23

Weight (kDa)

5.29

Isoelectric Point (pI)

41.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 20 - 93 2.8e-06 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 92
AccB1I GGYRCC 1 cut(s) 92
AccB7I CCANNNNNTGG 1 cut(s) 82
AccII CGCG 1 cut(s) 131
AciI CCGC 5 cut(s) 129, 131, 216, 224, 495
AclI AACGTT 2 cut(s) 242, 425
AcsI RAATTY 1 cut(s) 18
AcuI CTGAAG 1 cut(s) 404
AfaI GTAC 3 cut(s) 94, 364, 380
AfiI CCNNNNNNNGG 1 cut(s) 82
AflIII ACRYGT 2 cut(s) 79, 108
AgsI TTSAA 2 cut(s) 247, 259
AjnI CCWGG 1 cut(s) 156
AluBI AGCT 2 cut(s) 319, 488
AluI AGCT 2 cut(s) 319, 488
Alw21I GWGCWC 1 cut(s) 321
Alw26I GTCTC 1 cut(s) 402
ApoI RAATTY 1 cut(s) 18
Asp718I GGTACC 1 cut(s) 92
AspLEI GCGC 1 cut(s) 70
BanI GGYRCC 1 cut(s) 92
BanII GRGCYC 1 cut(s) 321
Bbv12I GWGCWC 1 cut(s) 321
BciT130I CCWGG 1 cut(s) 158
BclI TGATCA 1 cut(s) 171
BcoDI GTCTC 1 cut(s) 402
BglI GCCNNNNNGGC 1 cut(s) 224
BisI GCNGC 2 cut(s) 216, 225
BlsI GCNGC 2 cut(s) 217, 226
Bme1390I CCNGG 1 cut(s) 158
BmiI GGNNCC 2 cut(s) 94, 478
BmrFI CCNGG 1 cut(s) 158
BmsI GCATC 1 cut(s) 55
BpuEI CTTGAG 1 cut(s) 305
Bsa29I ATCGAT 1 cut(s) 462
BsaJI CCNNGG 4 cut(s) 129, 156, 178, 472
BsaXI ACNNNNNCTCC 2 cut(s) 468, 498
Bsc4I CCNNNNNNNGG 1 cut(s) 82
Bse3DI GCAATG 1 cut(s) 440
BseBI CCWGG 1 cut(s) 158
BseCI ATCGAT 1 cut(s) 462
BseDI CCNNGG 4 cut(s) 129, 156, 178, 472
BseGI GGATG 1 cut(s) 394
BseLI CCNNNNNNNGG 1 cut(s) 82
BseMI GCAATG 1 cut(s) 440
BsgI GTGCAG 1 cut(s) 272
Bsh1236I CGCG 1 cut(s) 131
BshNI GGYRCC 1 cut(s) 92
BshVI ATCGAT 1 cut(s) 462
BsiHKAI GWGCWC 1 cut(s) 321
BslI CCNNNNNNNGG 1 cut(s) 82
BsmAI GTCTC 1 cut(s) 402
Bsp1286I GDGCHC 1 cut(s) 321
Bsp143I GATC 1 cut(s) 171
BspACI CCGC 5 cut(s) 129, 131, 216, 224, 495
BspDI ATCGAT 1 cut(s) 462
BspFNI CGCG 1 cut(s) 131
BspLI GGNNCC 2 cut(s) 94, 478
BspT107I GGYRCC 1 cut(s) 92
BsrDI GCAATG 1 cut(s) 440
BssECI CCNNGG 4 cut(s) 129, 156, 178, 472
BssMI GATC 1 cut(s) 171
BssT1I CCWWGG 1 cut(s) 472
Bst2UI CCWGG 1 cut(s) 158
Bst4CI ACNGT 3 cut(s) 92, 284, 484
Bst6I CTCTTC 1 cut(s) 37
BstC8I GCNNGC 1 cut(s) 104
BstDEI CTNAG 2 cut(s) 228, 299
BstDSI CCRYGG 1 cut(s) 129
BstF5I GGATG 1 cut(s) 394
BstFNI CGCG 1 cut(s) 131
BstHHI GCGC 1 cut(s) 70
BstKTI GATC 1 cut(s) 174
BstMAI GTCTC 1 cut(s) 402
BstMBI GATC 1 cut(s) 171
BstMWI GCNNNNNNNGC 6 cut(s) 137, 215, 221, 224, 476, 485
BstNI CCWGG 1 cut(s) 158
BstNSI RCATGY 3 cut(s) 83, 106, 112
BstSCI CCNGG 1 cut(s) 156
BstUI CGCG 1 cut(s) 131
Bsu15I ATCGAT 1 cut(s) 462
BsuTUI ATCGAT 1 cut(s) 462
BtgI CCRYGG 1 cut(s) 129
BtsCI GGATG 1 cut(s) 394
Cac8I GCNNGC 1 cut(s) 104
CfoI GCGC 1 cut(s) 70
Cfr42I CCGCGG 1 cut(s) 132
ClaI ATCGAT 1 cut(s) 462
Csp6I GTAC 3 cut(s) 93, 363, 379
CviAII CATG 5 cut(s) 80, 103, 109, 272, 430
CviJI RGCY 9 cut(s) 42, 119, 161, 209, 227, 238, 319, 479, 488
CviKI_1 RGCY 9 cut(s) 42, 119, 161, 209, 227, 238, 319, 479, 488
CviQI GTAC 3 cut(s) 93, 363, 379
DdeI CTNAG 2 cut(s) 228, 299
DpnI GATC 1 cut(s) 173
DpnII GATC 1 cut(s) 171
Eam1104I CTCTTC 1 cut(s) 37
EarI CTCTTC 1 cut(s) 37
Ecl136II GAGCTC 1 cut(s) 319
Eco130I CCWWGG 1 cut(s) 472
Eco24I GRGCYC 1 cut(s) 321
Eco32I GATATC 1 cut(s) 370
Eco53kI GAGCTC 1 cut(s) 319
Eco57I CTGAAG 1 cut(s) 404
EcoICRI GAGCTC 1 cut(s) 319
EcoRII CCWGG 1 cut(s) 156
EcoRV GATATC 1 cut(s) 370
EcoT14I CCWWGG 1 cut(s) 472
EcoT38I GRGCYC 1 cut(s) 321
ErhI CCWWGG 1 cut(s) 472
FaeI CATG 5 cut(s) 83, 106, 112, 275, 433
FatI CATG 5 cut(s) 79, 102, 108, 271, 429
FauI CCCGC 2 cut(s) 136, 488
FbaI TGATCA 1 cut(s) 171
Fnu4HI GCNGC 2 cut(s) 216, 225
FokI GGATG 1 cut(s) 401
FriOI GRGCYC 1 cut(s) 321
Fsp4HI GCNGC 2 cut(s) 216, 225
GlaI GCGC 1 cut(s) 69
GluI GCNGC 2 cut(s) 216, 225
HhaI GCGC 1 cut(s) 70
Hin1II CATG 5 cut(s) 83, 106, 112, 275, 433
Hin6I GCGC 1 cut(s) 68
HinP1I GCGC 1 cut(s) 68
HinfI GANTC 1 cut(s) 326
Hpy188I TCNGA 2 cut(s) 16, 398
Hpy188III TCNNGA 1 cut(s) 169
HpyAV CCTTC 3 cut(s) 106, 241, 379
HpyCH4III ACNGT 3 cut(s) 92, 284, 484
HpyCH4IV ACGT 2 cut(s) 242, 425
HpyCH4V TGCA 5 cut(s) 102, 106, 253, 341, 445
HpyF10VI GCNNNNNNNGC 6 cut(s) 137, 215, 221, 224, 476, 485
HpyF3I CTNAG 2 cut(s) 228, 299
HpySE526I ACGT 2 cut(s) 242, 425
Hsp92II CATG 5 cut(s) 83, 106, 112, 275, 433
HspAI GCGC 1 cut(s) 68
KpnI GGTACC 1 cut(s) 96
Ksp22I TGATCA 1 cut(s) 171
KspI CCGCGG 1 cut(s) 132
Kzo9I GATC 1 cut(s) 171
LmnI GCTCC 2 cut(s) 316, 476
LpnPI CCDG 4 cut(s) 143, 170, 281, 395
LweI GCATC 1 cut(s) 55
MaeII ACGT 2 cut(s) 242, 425
MaeIII GTNAC 1 cut(s) 133
MalI GATC 1 cut(s) 173
MboI GATC 1 cut(s) 171
MboII GAAGA 2 cut(s) 24, 27
MhlI GDGCHC 1 cut(s) 321
MluCI AATT 1 cut(s) 18
MmeI TCCRAC 1 cut(s) 376
MnlI CCTC 5 cut(s) 58, 135, 188, 392, 427
MseI TTAA 1 cut(s) 44
MslI CAYNNNNRTG 3 cut(s) 107, 276, 434
MspA1I CMGCKG 1 cut(s) 131
MspR9I CCNGG 1 cut(s) 158
MvaI CCWGG 1 cut(s) 158
MvnI CGCG 1 cut(s) 131
MwoI GCNNNNNNNGC 6 cut(s) 137, 215, 221, 224, 476, 485
NdeII GATC 1 cut(s) 171
NlaIII CATG 5 cut(s) 83, 106, 112, 275, 433
NlaIV GGNNCC 2 cut(s) 94, 478
NmeAIII GCCGAG 1 cut(s) 159
NspI RCATGY 3 cut(s) 83, 106, 112
PaeI GCATGC 1 cut(s) 106
PciI ACATGT 2 cut(s) 79, 108
PfeI GAWTC 1 cut(s) 326
PflMI CCANNNNNTGG 1 cut(s) 82
PkrI GCNGC 2 cut(s) 217, 226
PscI ACATGT 2 cut(s) 79, 108
Psp124BI GAGCTC 1 cut(s) 321
Psp1406I AACGTT 2 cut(s) 242, 425
Psp6I CCWGG 1 cut(s) 156
PspGI CCWGG 1 cut(s) 156
PspN4I GGNNCC 2 cut(s) 94, 478
RsaI GTAC 3 cut(s) 94, 364, 380
RsaNI GTAC 3 cut(s) 93, 363, 379
RseI CAYNNNNRTG 3 cut(s) 107, 276, 434
SacI GAGCTC 1 cut(s) 321
SacII CCGCGG 1 cut(s) 132
SaqAI TTAA 1 cut(s) 44
SatI GCNGC 2 cut(s) 216, 225
Sau3AI GATC 1 cut(s) 171
ScrFI CCNGG 1 cut(s) 158
SduI GDGCHC 1 cut(s) 321
SfaNI GCATC 1 cut(s) 55
Sfr303I CCGCGG 1 cut(s) 132
SgrBI CCGCGG 1 cut(s) 132
SmiMI CAYNNNNRTG 3 cut(s) 107, 276, 434
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
SphI GCATGC 1 cut(s) 106
Sse9I AATT 1 cut(s) 18
SsiI CCGC 5 cut(s) 129, 131, 216, 224, 495
SstI GAGCTC 1 cut(s) 321
StyD4I CCNGG 1 cut(s) 156
StyI CCWWGG 1 cut(s) 472
TaaI ACNGT 3 cut(s) 92, 284, 484
TaiI ACGT 2 cut(s) 245, 428
TaqI TCGA 1 cut(s) 462
TasI AATT 1 cut(s) 18
TauI GCSGC 2 cut(s) 218, 227
TfiI GAWTC 1 cut(s) 326
Tru1I TTAA 1 cut(s) 44
Tru9I TTAA 1 cut(s) 44
TspDTI ATGAA 2 cut(s) 288, 418
TspGWI ACGGA 1 cut(s) 380
Van91I CCANNNNNTGG 1 cut(s) 82
XapI RAATTY 1 cut(s) 18
XceI RCATGY 3 cut(s) 83, 106, 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.