MD10G1032600.v1.1

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0005336
Physical Location & Seq
Reverse (-)
4197806 .. 4198333
528 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1032600.v1.1.491

Sequence Viewer

Length: 528 bp
ATGGAGTCCCCTCTTGGTTATGCGTTAACATTATTCTTTGCTGGTTATTTGTTTTTCTTCCTCTCTTCTCCTTCACCAGCCAATGCATCAGCCCAATTGGTCGATGGTGTATGCCAAAAATCCATAAATAATACTGAGTGCATGGAAGTTCTGCACTCTGATCCTCGAGTAAAATCGGCATCCACTTACATACTTCTTGCTCAAGTCGCCCTCGACCTAGCCATAGGGAATGCAAAAGATAGCCAAGCATTCATCAACAATTTGCTCAAGAGTGATCCGACGGAGCCTATTAAACAATGTGCTTCTTCATACGAGTTAGTGGTGGCTTCATTCCAAAGCGCCAAAGTTGAGATATACGAGGATCCTTTGACGGCCAACTATGATGTCAAAATTGCGGGCGATGATGCGGGTAACTGCGAAACTGCCTTAAGTTCTAAAGGAGTTAAATATCCTGAATTATCTGCTAGAAACCATGTTGTACTGTTATATAGTAGTATTGGGGATGTAATCACTGGTCAGATTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

176

Amino Acids

18.78

Weight (kDa)

4.55

Isoelectric Point (pI)

37.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 33 - 149 2.3e-12 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 395, 407
AclWI GGATC 4 cut(s) 155, 269, 356, 369
AcoI YGGCCR 1 cut(s) 372
AfaI GTAC 1 cut(s) 480
AflII CTTAAG 1 cut(s) 427
AlwI GGATC 4 cut(s) 155, 269, 356, 369
Ama87I CYCGRG 1 cut(s) 165
AoxI GGCC 1 cut(s) 372
AspLEI GCGC 1 cut(s) 341
AsuHPI GGTGA 1 cut(s) 66
AvaI CYCGRG 1 cut(s) 165
BamHI GGATCC 1 cut(s) 361
BccI CCATC 1 cut(s) 98
BceAI ACGGC 1 cut(s) 387
BfaI CTAG 2 cut(s) 218, 465
BfoI RGCGCY 1 cut(s) 342
BfrI CTTAAG 1 cut(s) 427
BmeT110I CYCGRG 1 cut(s) 165
BmiI GGNNCC 2 cut(s) 285, 363
BmsI GCATC 3 cut(s) 95, 188, 394
BpuEI CTTGAG 2 cut(s) 186, 251
Bse1I ACTGG 1 cut(s) 517
BseGI GGATG 2 cut(s) 179, 508
BseMII CTCAG 1 cut(s) 126
BseNI ACTGG 1 cut(s) 517
BsgI GTGCAG 1 cut(s) 137
BshFI GGCC 1 cut(s) 374
BsiHKCI CYCGRG 1 cut(s) 165
BsmI GAATGC 2 cut(s) 235, 248
BsnI GGCC 1 cut(s) 374
BsoBI CYCGRG 1 cut(s) 165
Bsp143I GATC 3 cut(s) 160, 274, 361
BspACI CCGC 2 cut(s) 395, 407
BspANI GGCC 1 cut(s) 374
BspCNI CTCAG 1 cut(s) 127
BspLI GGNNCC 2 cut(s) 285, 363
BspPI GGATC 4 cut(s) 155, 269, 356, 369
BspTI CTTAAG 1 cut(s) 427
BsrI ACTGG 1 cut(s) 517
BssMI GATC 3 cut(s) 160, 274, 361
Bst4CI ACNGT 1 cut(s) 483
Bst6I CTCTTC 1 cut(s) 70
BstAFI CTTAAG 1 cut(s) 427
BstC8I GCNNGC 1 cut(s) 397
BstDEI CTNAG 1 cut(s) 135
BstF5I GGATG 2 cut(s) 179, 508
BstH2I RGCGCY 1 cut(s) 342
BstHHI GCGC 1 cut(s) 341
BstKTI GATC 3 cut(s) 163, 277, 364
BstMBI GATC 3 cut(s) 160, 274, 361
BstMWI GCNNNNNNNGC 1 cut(s) 206
BstX2I RGATCY 1 cut(s) 361
BstYI RGATCY 1 cut(s) 361
BsuRI GGCC 1 cut(s) 374
BtgZI GCGATG 1 cut(s) 414
BtsCI GGATG 2 cut(s) 179, 508
BtsIMutI CAGTG 1 cut(s) 510
Cac8I GCNNGC 1 cut(s) 397
CfoI GCGC 1 cut(s) 341
Csp6I GTAC 1 cut(s) 479
CviAII CATG 2 cut(s) 142, 473
CviJI RGCY 7 cut(s) 80, 92, 221, 243, 286, 326, 374
CviKI_1 RGCY 7 cut(s) 80, 92, 221, 243, 286, 326, 374
CviQI GTAC 1 cut(s) 479
DdeI CTNAG 1 cut(s) 135
DpnI GATC 3 cut(s) 162, 276, 363
DpnII GATC 3 cut(s) 160, 274, 361
EaeI YGGCCR 1 cut(s) 372
Eam1104I CTCTTC 1 cut(s) 70
EarI CTCTTC 1 cut(s) 70
Eco88I CYCGRG 1 cut(s) 165
EcoT22I ATGCAT 1 cut(s) 88
FaeI CATG 2 cut(s) 145, 476
FatI CATG 2 cut(s) 141, 472
FauI CCCGC 2 cut(s) 388, 400
FokI GGATG 2 cut(s) 166, 515
FspBI CTAG 2 cut(s) 218, 465
GlaI GCGC 1 cut(s) 340
HaeII RGCGCY 1 cut(s) 342
HaeIII GGCC 1 cut(s) 374
HhaI GCGC 1 cut(s) 341
Hin1II CATG 2 cut(s) 145, 476
Hin6I GCGC 1 cut(s) 339
HinP1I GCGC 1 cut(s) 339
HincII GTYRAC 1 cut(s) 27
HindII GTYRAC 1 cut(s) 27
HinfI GANTC 1 cut(s) 5
HpaI GTTAAC 1 cut(s) 27
HphI GGTGA 1 cut(s) 66
Hpy166II GTNNAC 1 cut(s) 27
Hpy188I TCNGA 3 cut(s) 160, 279, 519
Hpy188III TCNNGA 2 cut(s) 268, 452
Hpy8I GTNNAC 1 cut(s) 27
Hpy99I CGWCG 1 cut(s) 283
HpyAV CCTTC 1 cut(s) 81
HpyCH4III ACNGT 1 cut(s) 483
HpyCH4V TGCA 4 cut(s) 86, 141, 154, 233
HpyF10VI GCNNNNNNNGC 1 cut(s) 206
HpyF3I CTNAG 1 cut(s) 135
Hsp92II CATG 2 cut(s) 145, 476
HspAI GCGC 1 cut(s) 339
KspAI GTTAAC 1 cut(s) 27
Kzo9I GATC 3 cut(s) 160, 274, 361
LmnI GCTCC 1 cut(s) 283
LpnPI CCDG 4 cut(s) 27, 90, 465, 498
LweI GCATC 3 cut(s) 95, 188, 394
MaeI CTAG 2 cut(s) 218, 465
MaeIII GTNAC 1 cut(s) 410
MalI GATC 3 cut(s) 162, 276, 363
MboI GATC 3 cut(s) 160, 274, 361
MboII GAAGA 3 cut(s) 49, 57, 297
MfeI CAATTG 1 cut(s) 95
MflI RGATCY 1 cut(s) 361
MluCI AATT 4 cut(s) 95, 259, 390, 455
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 1 cut(s) 302
MnlI CCTC 5 cut(s) 21, 71, 174, 221, 352
Mph1103I ATGCAT 1 cut(s) 88
MseI TTAA 4 cut(s) 26, 291, 428, 444
MspCI CTTAAG 1 cut(s) 427
MunI CAATTG 1 cut(s) 95
Mva1269I GAATGC 2 cut(s) 235, 248
MwoI GCNNNNNNNGC 1 cut(s) 206
NdeII GATC 3 cut(s) 160, 274, 361
NlaIII CATG 2 cut(s) 145, 476
NlaIV GGNNCC 2 cut(s) 285, 363
NsiI ATGCAT 1 cut(s) 88
PaeR7I CTCGAG 1 cut(s) 165
PctI GAATGC 2 cut(s) 235, 248
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
PspN4I GGNNCC 2 cut(s) 285, 363
PspXI VCTCGAGB 1 cut(s) 165
PsuI RGATCY 1 cut(s) 361
RsaI GTAC 1 cut(s) 480
RsaNI GTAC 1 cut(s) 479
SaqAI TTAA 4 cut(s) 26, 291, 428, 444
Sau3AI GATC 3 cut(s) 160, 274, 361
SchI GAGTC 1 cut(s) 14
SetI ASST 1 cut(s) 219
SfaNI GCATC 3 cut(s) 95, 188, 394
Sfr274I CTCGAG 1 cut(s) 165
SlaI CTCGAG 1 cut(s) 165
SmlI CTYRAG 4 cut(s) 165, 201, 266, 427
SmoI CTYRAG 4 cut(s) 165, 201, 266, 427
Sse9I AATT 4 cut(s) 95, 259, 390, 455
SsiI CCGC 2 cut(s) 395, 407
SspMI CTAG 2 cut(s) 218, 465
TaaI ACNGT 1 cut(s) 483
TaqI TCGA 3 cut(s) 102, 166, 213
TasI AATT 4 cut(s) 95, 259, 390, 455
TatI WGTACW 1 cut(s) 478
Tru1I TTAA 4 cut(s) 26, 291, 428, 444
Tru9I TTAA 4 cut(s) 26, 291, 428, 444
TscAI CASTG 1 cut(s) 517
TspDTI ATGAA 3 cut(s) 241, 297, 318
TspGWI ACGGA 1 cut(s) 296
TspRI CASTG 1 cut(s) 517
Vha464I CTTAAG 1 cut(s) 427
XcmI CCANNNNNNNNNTGG 1 cut(s) 101
XhoI CTCGAG 1 cut(s) 165
XspI CTAG 2 cut(s) 218, 465
Zsp2I ATGCAT 1 cut(s) 88
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.