AT5G50050

Invertase pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005336
Physical Location & Seq
Forward (+)
20360015 .. 20361031
1017 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50050.1

Sequence Viewer

Length: 528 bp
ATGGCTTCTTCATCCTACGGCTTTGGTGTCGTGTATCTGGCGGTTCTGTTACCATGTCTCTTAGTCCCTGCTTCGGCCACAAAGTATATCGACGCCATATGCCATCTTGTTTCCGACAAAGCCTTCTGCACCAAAACCTTGAATGCTTACCCTCCCGCGACTTCTGCCACCAGCACGTTCGAAGCGGCTGTGGCCACGCTTCACCTAGGAGTGTCTTATGCCAATAACGCTGCGGGTTTCGCCGGAGAAGCGGCAAAGGAAAATCCGCAGTTGAAGGAGAAGTTTGATGCATGTCAACTTGAACTTGCGCTCATTAGCAGGGGTCTCAAGAACGCTTCGGAGGAACTCTATGATGACACTGCGTACTTTGACGTCACGGATTGTCTCGACAACATAGCGACGATGAAGAATTTGGTCGGGAAAAATACAGACAAGGCTTCGAAAACTATCATAACGATGACATTGATGTTGGAGAATTTTATCTTTCTCGCCATTGGAGCCACCTCAACTATTGATATTAAAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

175

Amino Acids

18.65

Weight (kDa)

4.93

Isoelectric Point (pI)

30.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 29 - 129 7.1e-07 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 375
AccII CGCG 1 cut(s) 158
AciI CCGC 6 cut(s) 41, 156, 185, 233, 251, 266
AcoI YGGCCR 2 cut(s) 75, 192
AcsI RAATTY 2 cut(s) 409, 475
AcyI GRCGYC 2 cut(s) 93, 372
AfaI GTAC 1 cut(s) 365
AfiI CCNNNNNNNGG 1 cut(s) 73
AgsI TTSAA 3 cut(s) 142, 274, 302
Alw26I GTCTC 3 cut(s) 62, 329, 389
AoxI GGCC 2 cut(s) 75, 192
ApeKI GCWGC 1 cut(s) 230
ApoI RAATTY 2 cut(s) 409, 475
AspA2I CCTAGG 1 cut(s) 205
AspLEI GCGC 1 cut(s) 310
AsuHPI GGTGA 1 cut(s) 194
AsuII TTCGAA 2 cut(s) 180, 440
AvrII CCTAGG 1 cut(s) 205
BalI TGGCCA 1 cut(s) 194
BbvI GCAGC 1 cut(s) 217
BccI CCATC 1 cut(s) 111
BceAI ACGGC 1 cut(s) 34
BcoDI GTCTC 3 cut(s) 62, 329, 389
BfaI CTAG 1 cut(s) 206
BisI GCNGC 3 cut(s) 186, 231, 252
BlnI CCTAGG 1 cut(s) 205
BlsI GCNGC 3 cut(s) 187, 232, 253
BmiI GGNNCC 1 cut(s) 499
BmsI GCATC 1 cut(s) 277
Bpu14I TTCGAA 2 cut(s) 180, 440
BpuEI CTTGAG 1 cut(s) 311
BsaHI GRCGYC 2 cut(s) 93, 372
BsaI GGTCTC 1 cut(s) 329
BsaJI CCNNGG 1 cut(s) 205
Bsc4I CCNNNNNNNGG 1 cut(s) 73
BseDI CCNNGG 1 cut(s) 205
BseGI GGATG 1 cut(s) 11
BseLI CCNNNNNNNGG 1 cut(s) 73
BseXI GCAGC 1 cut(s) 217
BsgI GTGCAG 1 cut(s) 112
Bsh1236I CGCG 1 cut(s) 158
BshFI GGCC 2 cut(s) 77, 194
BsiSI CCGG 1 cut(s) 243
BslFI GGGAC 1 cut(s) 50
BslI CCNNNNNNNGG 1 cut(s) 73
BsmAI GTCTC 3 cut(s) 62, 329, 389
BsmFI GGGAC 1 cut(s) 50
BsmI GAATGC 1 cut(s) 148
BsnI GGCC 2 cut(s) 77, 194
Bso31I GGTCTC 1 cut(s) 329
Bsp119I TTCGAA 2 cut(s) 180, 440
BspACI CCGC 6 cut(s) 41, 156, 185, 233, 251, 266
BspANI GGCC 2 cut(s) 77, 194
BspFNI CGCG 1 cut(s) 158
BspLI GGNNCC 1 cut(s) 499
BspT104I TTCGAA 2 cut(s) 180, 440
BspTNI GGTCTC 1 cut(s) 329
BssECI CCNNGG 1 cut(s) 205
BssNI GRCGYC 2 cut(s) 93, 372
BssT1I CCWWGG 1 cut(s) 205
BstACI GRCGYC 2 cut(s) 93, 372
BstBI TTCGAA 2 cut(s) 180, 440
BstDEI CTNAG 1 cut(s) 61
BstF5I GGATG 1 cut(s) 11
BstFNI CGCG 1 cut(s) 158
BstHHI GCGC 1 cut(s) 310
BstMAI GTCTC 3 cut(s) 62, 329, 389
BstMWI GCNNNNNNNGC 6 cut(s) 164, 191, 227, 239, 248, 497
BstNSI RCATGY 1 cut(s) 294
BstUI CGCG 1 cut(s) 158
BstV1I GCAGC 1 cut(s) 217
BsuRI GGCC 2 cut(s) 77, 194
BtsCI GGATG 1 cut(s) 11
BtsI GCAGTG 1 cut(s) 357
BtsIMutI CAGTG 1 cut(s) 357
CfoI GCGC 1 cut(s) 310
CseI GACGC 1 cut(s) 101
Csp6I GTAC 1 cut(s) 364
CviAII CATG 2 cut(s) 54, 291
CviJI RGCY 8 cut(s) 5, 21, 77, 122, 188, 194, 437, 500
CviKI_1 RGCY 8 cut(s) 5, 21, 77, 122, 188, 194, 437, 500
CviQI GTAC 1 cut(s) 364
DdeI CTNAG 1 cut(s) 61
EaeI YGGCCR 2 cut(s) 75, 192
Eco130I CCWWGG 1 cut(s) 205
Eco31I GGTCTC 1 cut(s) 329
EcoT14I CCWWGG 1 cut(s) 205
EcoT22I ATGCAT 1 cut(s) 292
ErhI CCWWGG 1 cut(s) 205
FaeI CATG 2 cut(s) 57, 294
FaqI GGGAC 1 cut(s) 50
FatI CATG 2 cut(s) 53, 290
FauI CCCGC 2 cut(s) 163, 226
FauNDI CATATG 1 cut(s) 98
Fnu4HI GCNGC 3 cut(s) 186, 231, 252
Fsp4HI GCNGC 3 cut(s) 186, 231, 252
FspBI CTAG 1 cut(s) 206
GlaI GCGC 1 cut(s) 309
GluI GCNGC 3 cut(s) 186, 231, 252
HaeIII GGCC 2 cut(s) 77, 194
HapII CCGG 1 cut(s) 243
HgaI GACGC 1 cut(s) 101
HhaI GCGC 1 cut(s) 310
Hin1I GRCGYC 2 cut(s) 93, 372
Hin1II CATG 2 cut(s) 57, 294
Hin6I GCGC 1 cut(s) 308
HinP1I GCGC 1 cut(s) 308
HincII GTYRAC 1 cut(s) 296
HindII GTYRAC 1 cut(s) 296
HpaII CCGG 1 cut(s) 243
HphI GGTGA 1 cut(s) 194
Hpy166II GTNNAC 1 cut(s) 296
Hpy188I TCNGA 2 cut(s) 115, 340
Hpy188III TCNNGA 3 cut(s) 328, 386, 418
Hpy8I GTNNAC 1 cut(s) 296
Hpy99I CGWCG 2 cut(s) 95, 403
HpyAV CCTTC 2 cut(s) 133, 268
HpyCH4IV ACGT 2 cut(s) 176, 372
HpyCH4V TGCA 2 cut(s) 129, 290
HpyF10VI GCNNNNNNNGC 6 cut(s) 164, 191, 227, 239, 248, 497
HpyF3I CTNAG 1 cut(s) 61
HpySE526I ACGT 2 cut(s) 176, 372
Hsp92I GRCGYC 2 cut(s) 93, 372
Hsp92II CATG 2 cut(s) 57, 294
HspAI GCGC 1 cut(s) 308
LmnI GCTCC 1 cut(s) 497
LpnPI CCDG 5 cut(s) 23, 81, 184, 256, 304
Lsp1109I GCAGC 1 cut(s) 217
LweI GCATC 1 cut(s) 277
MaeI CTAG 1 cut(s) 206
MaeII ACGT 2 cut(s) 176, 372
MaeIII GTNAC 2 cut(s) 48, 373
MboII GAAGA 1 cut(s) 418
MlsI TGGCCA 1 cut(s) 194
MluCI AATT 2 cut(s) 409, 475
MluNI TGGCCA 1 cut(s) 194
MmeI TCCRAC 2 cut(s) 138, 450
MnlI CCTC 3 cut(s) 162, 334, 514
Mox20I TGGCCA 1 cut(s) 194
Mph1103I ATGCAT 1 cut(s) 292
MscI TGGCCA 1 cut(s) 194
MseI TTAA 1 cut(s) 519
MslI CAYNNNNRTG 1 cut(s) 455
Msp20I TGGCCA 1 cut(s) 194
MspI CCGG 1 cut(s) 243
Mva1269I GAATGC 1 cut(s) 148
MvnI CGCG 1 cut(s) 158
MwoI GCNNNNNNNGC 6 cut(s) 164, 191, 227, 239, 248, 497
NdeI CATATG 1 cut(s) 98
NlaIII CATG 2 cut(s) 57, 294
NlaIV GGNNCC 1 cut(s) 499
NmuCI GTSAC 1 cut(s) 373
NsiI ATGCAT 1 cut(s) 292
NspI RCATGY 1 cut(s) 294
NspV TTCGAA 2 cut(s) 180, 440
PctI GAATGC 1 cut(s) 148
PkrI GCNGC 3 cut(s) 187, 232, 253
PspN4I GGNNCC 1 cut(s) 499
RsaI GTAC 1 cut(s) 365
RsaNI GTAC 1 cut(s) 364
RseI CAYNNNNRTG 1 cut(s) 455
SaqAI TTAA 1 cut(s) 519
SatI GCNGC 3 cut(s) 186, 231, 252
SetI ASST 5 cut(s) 140, 179, 207, 375, 506
SfaNI GCATC 1 cut(s) 277
SfuI TTCGAA 2 cut(s) 180, 440
SmiMI CAYNNNNRTG 1 cut(s) 455
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
Sse9I AATT 2 cut(s) 409, 475
SsiI CCGC 6 cut(s) 41, 156, 185, 233, 251, 266
SspMI CTAG 1 cut(s) 206
StyI CCWWGG 1 cut(s) 205
TaiI ACGT 2 cut(s) 179, 375
TaqI TCGA 4 cut(s) 90, 180, 387, 440
TasI AATT 2 cut(s) 409, 475
TauI GCSGC 2 cut(s) 188, 254
Tru1I TTAA 1 cut(s) 519
Tru9I TTAA 1 cut(s) 519
TscAI CASTG 1 cut(s) 364
TseFI GTSAC 1 cut(s) 373
TseI GCWGC 1 cut(s) 230
Tsp45I GTSAC 1 cut(s) 373
TspDTI ATGAA 1 cut(s) 419
TspGWI ACGGA 1 cut(s) 392
TspRI CASTG 1 cut(s) 364
XapI RAATTY 2 cut(s) 409, 475
XceI RCATGY 1 cut(s) 294
XmaJI CCTAGG 1 cut(s) 205
XspI CTAG 1 cut(s) 206
ZraI GACGTC 1 cut(s) 373
Zsp2I ATGCAT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.