Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002229

Physical Location & Seq

Genomic Coordinates Reverse (-)

7162931 .. 7165410

Gene Structure 2480 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G16530.4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1032 bp

ATGGTGGAATTGGAGATTGTGTGGTTGGTTAGAGGCGCTTGGATCACTGTTTGGATCGTCTCTATACTTCCTTTGGTGATTGCTTCGATACCCACTTCAAAGCTCAACTCTTTTCGTGAACTCGTTTTGAGTTTCGCTGGTAGAGGCAAGATTCTTCACCCTTCTTCTCAGAAGTTTACAATTCCTCAGAAATGCTTTGCACACTTTTATGTTATTGGAGTGGTGTGGACAACTCTCCTGCTTGCTGCAACTTGGATGTATGCTTGCAAAATGGCGCCTTTATCTTCTGAGGAGTTCCAGTTATCCGACATTGCAAGCCGTTTAGCCGGAGGTTCAGATGTTTTCTCCGTTCATAAATCTAATATGACTCCTGTCGAGCATCGGTTTAAAGTGTGGCGAGCGGTGTTTCTACTTCTTCTGATGGAGATCCATGTCTTGAGACGGCTTATAGAGTCATTCTATGTTTTCAAATATAGCCCTTCTGCTCGGATGCACATTCTCGGTTATTTCGCAGGATTGTTTTTCTATGTAACAGCTCCTTTGTCACTCTGCTCCAATATAGCTCCAGAGGTAGCAGGATTCGTAGGAAATCAAGTGGCTGAGTTCATTGCTAATGGGAAAAGCCATACTTCAGCACCTGAATTTAACTTGTTATCATCTATAAGTCCTTTGATGAAGCTTGGATCACTCCAGTGGATTGGTGGAGCCATTTTTCTTTGGGGATGGATACATCAGCGCCGCTGTCACGCCATTCTTGGATCGCTCCGGGAAAATCCGAGCCAAGCGAAAGAGTACATTATTCCGTATGGAGATTGGTTTGGGATGGTGTCTTCTCCACATTTCTTGGCAGAAATTGTTTTGTATGCGGGTTTACTGATTGCGAGTGGAGGAACAGATATAACCATCTGGTTACTCTTTGGTTTTGTGGCTGCTAATCTGACATACGCGGCTGGAGAAACGCACCGTTGGTATCTCCGGAAGTTTGAGAATTATCCGGCTAATCGGCATGCCATTTTCCCTTATGTCTACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003865 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0006066 GO:0006464 GO:0006486 GO:0006487 GO:0006629 GO:0006720 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0012505 GO:0016093 GO:0016229 GO:0016491 GO:0016627 GO:0019538 GO:0033765 GO:0034645 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0055114 GO:0070085 GO:0071704 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko00140 map00140

Pfam Domains

Protein Families

Steroid_dh

Protein Analysis

343

Amino Acids

38.68

Weight (kDa)

9.2

Isoelectric Point (pI)

49.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Steroid_dh	PF02544	232 - 343	1.4e-22	3-oxo-5-alpha-steroid 4-dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002229)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G72590 AT2G16530 AT2G16530 AT2G16530 AT2G16530 AT2G16530 AT3G43840 AT3G43840
fragaria_vesca	FvH4_2g10631 FvH4_2g10631 FvH4_2g10631 FvH4_2g10640 FvH4_2g10640 FvH4_2g10640
malus_domestica	MD05G1054700.v1.1 MD10G1062200.v1.1
prunus_persica	Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1
pyrus_communis	pycom05g04490 pycom05g04510 pycom10g04790
rosa_chinensis	RchiOBHm_Chr6g0268311 RchiOBHm_Chr6g0268321 RchiOBHm_Chr6g0268341
rosa_multiflora	Rmu_sc0009812.1_g000007 Rmu_sc0010202.1_g000011
rosa_roxburghii	Rroxscaffold_7G00199860 Rroxscaffold_7G00199880
rosa_rugosa	Rorug06G0040400 Rorug06G0040400 Rorug06G0040400
rosa_samantha	Rh6AG160500 Rh6BG164800 Rh6BG164900 Rh6CG153500 Rh6DG150600
rosa_wichuraiana	Rw0G000500 Rw6G013880 Rw6G013890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	274
AccBSI	CCGCTC	1 cut(s)	401
AccI	GTMKAC	1 cut(s)	1026
AccII	CGCG	1 cut(s)	947
AccIII	TCCGGA	1 cut(s)	975
AciI	CCGC	4 cut(s)	401, 739, 866, 947
AclWI	GGATC	5 cut(s)	50, 62, 421, 691, 766
AcsI	RAATTY	1 cut(s)	641
AcuI	CTGAAG	1 cut(s)	615
AcyI	GRCGYC	1 cut(s)	275
AfaI	GTAC	1 cut(s)	794
AgsI	TTSAA	2 cut(s)	99, 469
AleI	CACNNNNGTG	1 cut(s)	691
AluBI	AGCT	4 cut(s)	103, 536, 563, 679
AluI	AGCT	4 cut(s)	103, 536, 563, 679
Alw26I	GTCTC	2 cut(s)	64, 433
AlwI	GGATC	5 cut(s)	50, 62, 421, 691, 766
AlwNI	CAGNNNCTG	1 cut(s)	638
Aor13HI	TCCGGA	1 cut(s)	975
ApeKI	GCWGC	2 cut(s)	245, 929
ApoI	RAATTY	1 cut(s)	641
AspLEI	GCGC	3 cut(s)	38, 277, 738
AsuC2I	CCSGG	1 cut(s)	767
AsuHPI	GGTGA	2 cut(s)	88, 149
BanI	GGYRCC	1 cut(s)	274
BbsI	GAAGAC	1 cut(s)	822
BbvI	GCAGC	2 cut(s)	232, 916
BccI	CCATC	4 cut(s)	415, 717, 817, 911
BceAI	ACGGC	2 cut(s)	303, 458
BciVI	GTATCC	1 cut(s)	720
BcnI	CCSGG	1 cut(s)	767
BcoDI	GTCTC	2 cut(s)	64, 433
BfoI	RGCGCY	3 cut(s)	39, 278, 739
BfuI	GTATCC	1 cut(s)	720
BisI	GCNGC	4 cut(s)	246, 739, 930, 948
BlsI	GCNGC	4 cut(s)	247, 740, 931, 949
Bme1390I	CCNGG	1 cut(s)	767
BmiI	GGNNCC	2 cut(s)	276, 706
BmrFI	CCNGG	1 cut(s)	767
BmsI	GCATC	2 cut(s)	388, 480
BoxI	GACNNNNGTC	1 cut(s)	371
BpiI	GAAGAC	1 cut(s)	822
BpmI	CTGGAG	3 cut(s)	549, 674, 972
BpuEI	CTTGAG	1 cut(s)	457
BpuMI	CCSGG	1 cut(s)	767
BsaBI	GATNNNNATC	1 cut(s)	425
BsaHI	GRCGYC	1 cut(s)	275
BsaWI	WCCGGW	1 cut(s)	975
BsaXI	ACNNNNNCTCC	4 cut(s)	5, 35, 284, 314
Bse1I	ACTGG	2 cut(s)	298, 691
Bse3DI	GCAATG	2 cut(s)	309, 606
Bse8I	GATNNNNATC	1 cut(s)	425
BseAI	TCCGGA	1 cut(s)	975
BseGI	GGATG	4 cut(s)	261, 495, 728, 828
BseJI	GATNNNNATC	1 cut(s)	425
BseMI	GCAATG	2 cut(s)	309, 606
BseMII	CTCAG	4 cut(s)	182, 200, 279, 591
BseNI	ACTGG	2 cut(s)	298, 691
BseRI	GAGGAG	1 cut(s)	305
BseXI	GCAGC	2 cut(s)	232, 916
Bsh1236I	CGCG	1 cut(s)	947
BshNI	GGYRCC	1 cut(s)	274
BsiSI	CCGG	4 cut(s)	327, 766, 976, 995
BsmAI	GTCTC	2 cut(s)	64, 433
BsmBI	CGTCTC	2 cut(s)	64, 433
Bsp13I	TCCGGA	1 cut(s)	975
Bsp143I	GATC	5 cut(s)	42, 54, 426, 683, 758
BspACI	CCGC	4 cut(s)	401, 739, 866, 947
BspCNI	CTCAG	4 cut(s)	181, 199, 280, 592
BspEI	TCCGGA	1 cut(s)	975
BspFNI	CGCG	1 cut(s)	947
BspLI	GGNNCC	2 cut(s)	276, 706
BspPI	GGATC	5 cut(s)	50, 62, 421, 691, 766
BspT107I	GGYRCC	1 cut(s)	274
BsrBI	CCGCTC	1 cut(s)	401
BsrDI	GCAATG	2 cut(s)	309, 606
BsrI	ACTGG	2 cut(s)	298, 691
BssMI	GATC	5 cut(s)	42, 54, 426, 683, 758
BssNI	GRCGYC	1 cut(s)	275
Bst4CI	ACNGT	2 cut(s)	49, 965
BstACI	GRCGYC	1 cut(s)	275
BstC8I	GCNNGC	5 cut(s)	243, 265, 316, 399, 1008
BstDEI	CTNAG	4 cut(s)	168, 186, 288, 600
BstF5I	GGATG	4 cut(s)	261, 495, 728, 828
BstFNI	CGCG	1 cut(s)	947
BstH2I	RGCGCY	3 cut(s)	39, 278, 739
BstHHI	GCGC	3 cut(s)	38, 277, 738
BstKTI	GATC	5 cut(s)	45, 57, 429, 686, 761
BstMAI	GTCTC	2 cut(s)	64, 433
BstMBI	GATC	5 cut(s)	42, 54, 426, 683, 758
BstNSI	RCATGY	1 cut(s)	1010
BstPAI	GACNNNNGTC	1 cut(s)	371
BstSCI	CCNGG	1 cut(s)	765
BstUI	CGCG	1 cut(s)	947
BstV1I	GCAGC	2 cut(s)	232, 916
BstV2I	GAAGAC	1 cut(s)	822
BstX2I	RGATCY	1 cut(s)	426
BstXI	CCANNNNNNTGG	1 cut(s)	698
BstYI	RGATCY	1 cut(s)	426
BsuI	GTATCC	1 cut(s)	720
BtsCI	GGATG	4 cut(s)	261, 495, 728, 828
BtsIMutI	CAGTG	2 cut(s)	45, 698
Cac8I	GCNNGC	5 cut(s)	243, 265, 316, 399, 1008
CaiI	CAGNNNCTG	1 cut(s)	638
CfoI	GCGC	3 cut(s)	38, 277, 738
Csp6I	GTAC	1 cut(s)	793
CspCI	CAANNNNNGTGG	2 cut(s)	825, 860
CviAII	CATG	2 cut(s)	431, 1007
CviQI	GTAC	1 cut(s)	793
DdeI	CTNAG	4 cut(s)	168, 186, 288, 600
DinI	GGCGCC	1 cut(s)	276
DpnI	GATC	5 cut(s)	44, 56, 428, 685, 760
DpnII	GATC	5 cut(s)	42, 54, 426, 683, 758
DraI	TTTAAA	1 cut(s)	388
Eco57I	CTGAAG	1 cut(s)	615
EgeI	GGCGCC	1 cut(s)	276
EheI	GGCGCC	1 cut(s)	276
Esp3I	CGTCTC	2 cut(s)	64, 433
FaeI	CATG	2 cut(s)	434, 1010
FalI	AAGNNNNNCTT	2 cut(s)	613, 645
FatI	CATG	2 cut(s)	430, 1006
FauI	CCCGC	1 cut(s)	859
FblI	GTMKAC	1 cut(s)	1026
Fnu4HI	GCNGC	4 cut(s)	246, 739, 930, 948
FokI	GGATG	4 cut(s)	268, 502, 735, 835
Fsp4HI	GCNGC	4 cut(s)	246, 739, 930, 948
GlaI	GCGC	3 cut(s)	37, 276, 737
GluI	GCNGC	4 cut(s)	246, 739, 930, 948
GsuI	CTGGAG	3 cut(s)	549, 674, 972
HaeII	RGCGCY	3 cut(s)	39, 278, 739
HapII	CCGG	4 cut(s)	327, 766, 976, 995
HhaI	GCGC	3 cut(s)	38, 277, 738
Hin1I	GRCGYC	1 cut(s)	275
Hin1II	CATG	2 cut(s)	434, 1010
Hin6I	GCGC	3 cut(s)	36, 275, 736
HinP1I	GCGC	3 cut(s)	36, 275, 736
HindIII	AAGCTT	1 cut(s)	677
HinfI	GANTC	4 cut(s)	151, 367, 452, 579
HpaII	CCGG	4 cut(s)	327, 766, 976, 995
HphI	GGTGA	2 cut(s)	88, 149
Hpy166II	GTNNAC	5 cut(s)	119, 177, 228, 872, 1027
Hpy188I	TCNGA	9 cut(s)	171, 189, 289, 307, 337, 420, 489, 777, 939
Hpy188III	TCNNGA	4 cut(s)	116, 436, 566, 976
Hpy8I	GTNNAC	5 cut(s)	119, 177, 228, 872, 1027
HpyAV	CCTTC	2 cut(s)	171, 489
HpyCH4III	ACNGT	2 cut(s)	49, 965
HpyCH4V	TGCA	5 cut(s)	200, 248, 267, 314, 493
HpyF3I	CTNAG	4 cut(s)	168, 186, 288, 600
Hsp92I	GRCGYC	1 cut(s)	275
Hsp92II	CATG	2 cut(s)	434, 1010
HspAI	GCGC	3 cut(s)	36, 275, 736
KasI	GGCGCC	1 cut(s)	274
Kpn2I	TCCGGA	1 cut(s)	975
Kzo9I	GATC	5 cut(s)	42, 54, 426, 683, 758
LmnI	GCTCC	5 cut(s)	541, 557, 568, 704, 768
Lsp1109I	GCAGC	2 cut(s)	232, 916
LweI	GCATC	2 cut(s)	388, 480
MaeIII	GTNAC	4 cut(s)	529, 543, 743, 909
MalI	GATC	5 cut(s)	44, 56, 428, 685, 760
MbiI	CCGCTC	1 cut(s)	401
MboI	GATC	5 cut(s)	42, 54, 426, 683, 758
MboII	GAAGA	5 cut(s)	146, 156, 276, 407, 822
MflI	RGATCY	1 cut(s)	426
MluCI	AATT	5 cut(s)	8, 180, 641, 852, 988
Mly113I	GGCGCC	1 cut(s)	275
MlyI	GAGTC	2 cut(s)	361, 461
MmeI	TCCRAC	1 cut(s)	330
MnlI	CCTC	7 cut(s)	26, 137, 195, 283, 323, 562, 881
MroI	TCCGGA	1 cut(s)	975
MseI	TTAA	2 cut(s)	387, 645
MslI	CAYNNNNRTG	2 cut(s)	207, 691
MspA1I	CMGCKG	1 cut(s)	741
MspI	CCGG	4 cut(s)	327, 766, 976, 995
MspR9I	CCNGG	1 cut(s)	767
MvnI	CGCG	1 cut(s)	947
NarI	GGCGCC	1 cut(s)	275
NciI	CCSGG	1 cut(s)	767
NdeII	GATC	5 cut(s)	42, 54, 426, 683, 758
NlaIII	CATG	2 cut(s)	434, 1010
NlaIV	GGNNCC	2 cut(s)	276, 706
NmuCI	GTSAC	2 cut(s)	543, 743
NspI	RCATGY	1 cut(s)	1010
OliI	CACNNNNGTG	1 cut(s)	691
PaeI	GCATGC	1 cut(s)	1010
PfeI	GAWTC	2 cut(s)	151, 579
PfoI	TCCNGGA	1 cut(s)	765
PkrI	GCNGC	4 cut(s)	247, 740, 931, 949
PleI	GAGTC	2 cut(s)	361, 460
PluTI	GGCGCC	1 cut(s)	278
PpsI	GAGTC	2 cut(s)	361, 460
PshAI	GACNNNNGTC	1 cut(s)	371
PspN4I	GGNNCC	2 cut(s)	276, 706
PstNI	CAGNNNCTG	1 cut(s)	638
PsuI	RGATCY	1 cut(s)	426
RsaI	GTAC	1 cut(s)	794
RsaNI	GTAC	1 cut(s)	793
RseI	CAYNNNNRTG	2 cut(s)	207, 691
SaqAI	TTAA	2 cut(s)	387, 645
SatI	GCNGC	4 cut(s)	246, 739, 930, 948
Sau3AI	GATC	5 cut(s)	42, 54, 426, 683, 758
SchI	GAGTC	2 cut(s)	361, 461
ScrFI	CCNGG	1 cut(s)	767
SetI	ASST	7 cut(s)	105, 334, 538, 565, 573, 640, 681
SfaNI	GCATC	2 cut(s)	388, 480
SfoI	GGCGCC	1 cut(s)	276
SmiMI	CAYNNNNRTG	2 cut(s)	207, 691
SmlI	CTYRAG	1 cut(s)	436
SmoI	CTYRAG	1 cut(s)	436
SphI	GCATGC	1 cut(s)	1010
Sse9I	AATT	5 cut(s)	8, 180, 641, 852, 988
SsiI	CCGC	4 cut(s)	401, 739, 866, 947
SspDI	GGCGCC	1 cut(s)	274
StyD4I	CCNGG	1 cut(s)	765
TaaI	ACNGT	2 cut(s)	49, 965
TaqI	TCGA	2 cut(s)	86, 375
TasI	AATT	5 cut(s)	8, 180, 641, 852, 988
TatI	WGTACW	1 cut(s)	792
TauI	GCSGC	2 cut(s)	741, 950
TfiI	GAWTC	2 cut(s)	151, 579
Tru1I	TTAA	2 cut(s)	387, 645
Tru9I	TTAA	2 cut(s)	387, 645
TscAI	CASTG	2 cut(s)	52, 698
TseFI	GTSAC	2 cut(s)	543, 743
TseI	GCWGC	2 cut(s)	245, 929
Tsp45I	GTSAC	2 cut(s)	543, 743
TspDTI	ATGAA	3 cut(s)	341, 595, 689
TspGWI	ACGGA	2 cut(s)	337, 792
TspRI	CASTG	2 cut(s)	52, 698
XapI	RAATTY	1 cut(s)	641
XceI	RCATGY	1 cut(s)	1010
XcmI	CCANNNNNNNNNTGG	1 cut(s)	698
XmiI	GTMKAC	1 cut(s)	1026

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.