Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0002229

Physical Location & Seq

Genomic Coordinates Forward (+)

47158252 .. 47161881

Gene Structure 3630 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00199860.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 876 bp

ATGTCTTCTTCAAGGAGATTCACAGTTCCTCAAAAGTTCTTCTGCCATTTCTATGTGCTGGCTGTCGTGTGGACAACCCTACTTCTTGTTACAACTTGGATGTATGCATATAAAATGGTGCCAATGCATGTGGACCCATTTCTTTATCCTACATTCGCCAGCCGCTTTACTGGAGGTTCACATATCATTTCCTGGCGCAAGTCTCCATCAGTTCCTATGGGTTATAGATATAGTGTCTGGCGTTCTGTCTTTTTGCTTTTGTTGATGGAAGTTCAAGTTGTGAAGCGCCTTATTGAGACAATATATGTATTCAAGTATAGCTCCACTGCAAGGATGCACATCTTTGGGTATCTGACTGGCCTCTTTTTCTACACAGCTGCACCCTTGTCACTTTGCTGCAATAATTTTCTGGAGGTCTATAACTTTTCCGTTAATGGAGTGGCTGAATTTATTGTCAAAGGCAAGAAGAATACTATGGAAGATCTGGATTTTGATTCTTTGGAATTTATTAGTTCTCTTCTGAAGCTTGGTTGGCTCCAGTGGATTGGTGCTGCTATATTCTTTTGGGGTTGGATCCATCAGCGCCGCTGTCATGAAATTCTTGGCTCATTACGAGAACACAGTGAACAAAATGTATATGTGATTCCTCATGGGGATTGGTTTAAAATTGTATCATCTCCACATTATCTTGCTGAGATAGTTATATATGCCGGTCTTGTGGTTGCTAGTGGAGGAGCAGACCTCACAATCTGGTTACTATTTGGATTTGTGGTGTCAAACTTGGTGTTTGCAGCAGCAGAAACACATAGGTGGTACCTTCAAAAGTTCAAAAGTTACCCCAGAAATCGGCGTGCCATTATTCCATTTTTATACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003865 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0006066 GO:0006464 GO:0006486 GO:0006487 GO:0006629 GO:0006720 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0012505 GO:0016093 GO:0016229 GO:0016491 GO:0016627 GO:0019538 GO:0033765 GO:0034645 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0055114 GO:0070085 GO:0071704 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko00140 map00140

Pfam Domains

Protein Families

Steroid_dh

Protein Analysis

291

Amino Acids

33.94

Weight (kDa)

9.42

Isoelectric Point (pI)

52.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Steroid_dh	PF02544	180 - 291	9.6e-27	3-oxo-5-alpha-steroid 4-dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002229)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G72590 AT2G16530 AT2G16530 AT2G16530 AT2G16530 AT2G16530 AT3G43840 AT3G43840
fragaria_vesca	FvH4_2g10631 FvH4_2g10631 FvH4_2g10631 FvH4_2g10640 FvH4_2g10640 FvH4_2g10640
malus_domestica	MD05G1054700.v1.1 MD10G1062200.v1.1
prunus_persica	Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1
pyrus_communis	pycom05g04490 pycom05g04510 pycom10g04790
rosa_chinensis	RchiOBHm_Chr6g0268311 RchiOBHm_Chr6g0268321 RchiOBHm_Chr6g0268341
rosa_multiflora	Rmu_sc0009812.1_g000007 Rmu_sc0010202.1_g000011
rosa_roxburghii	Rroxscaffold_7G00199860 Rroxscaffold_7G00199880
rosa_rugosa	Rorug06G0040400 Rorug06G0040400 Rorug06G0040400
rosa_samantha	Rh6AG160500 Rh6BG164800 Rh6BG164900 Rh6CG153500 Rh6DG150600
rosa_wichuraiana	Rw0G000500 Rw6G013880 Rw6G013890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	813
AccB1I	GGYRCC	2 cut(s)	118, 813
AciI	CCGC	2 cut(s)	163, 586
AclWI	GGATC	2 cut(s)	568, 581
AcsI	RAATTY	3 cut(s)	446, 503, 597
AcuI	CTGAAG	1 cut(s)	542
AfaI	GTAC	1 cut(s)	815
AfiI	CCNNNNNNNGG	2 cut(s)	330, 846
AgsI	TTSAA	5 cut(s)	12, 275, 313, 821, 829
AjnI	CCWGG	1 cut(s)	191
AleI	CACNNNNGTG	1 cut(s)	808
AluBI	AGCT	3 cut(s)	321, 377, 526
AluI	AGCT	3 cut(s)	321, 377, 526
Alw26I	GTCTC	2 cut(s)	207, 290
AlwI	GGATC	2 cut(s)	568, 581
AoxI	GGCC	1 cut(s)	358
ApeKI	GCWGC	5 cut(s)	377, 396, 551, 791, 794
ApoI	RAATTY	3 cut(s)	446, 503, 597
Asp718I	GGTACC	1 cut(s)	813
AspLEI	GCGC	3 cut(s)	198, 288, 585
AspS9I	GGNCC	1 cut(s)	133
AvaII	GGWCC	1 cut(s)	133
BamHI	GGATCC	1 cut(s)	573
BanI	GGYRCC	2 cut(s)	118, 813
BbvI	GCAGC	5 cut(s)	364, 383, 538, 803, 806
BccI	CCATC	3 cut(s)	214, 259, 585
BciT130I	CCWGG	1 cut(s)	193
BcoDI	GTCTC	2 cut(s)	207, 290
BfaI	CTAG	1 cut(s)	726
BfoI	RGCGCY	2 cut(s)	289, 586
BglII	AGATCT	1 cut(s)	481
BisI	GCNGC	7 cut(s)	163, 378, 397, 552, 586, 792, 795
BlsI	GCNGC	7 cut(s)	164, 379, 398, 553, 587, 793, 796
Bme1390I	CCNGG	1 cut(s)	193
Bme18I	GGWCC	1 cut(s)	133
BmgT120I	GGNCC	1 cut(s)	133
BmiI	GGNNCC	5 cut(s)	120, 135, 536, 575, 815
BmrFI	CCNGG	1 cut(s)	193
BmsI	GCATC	1 cut(s)	324
BplI	GAGNNNNNCTC	2 cut(s)	726, 758
BpmI	CTGGAG	3 cut(s)	192, 431, 521
BsaBI	GATNNNNATC	1 cut(s)	338
Bsc4I	CCNNNNNNNGG	2 cut(s)	330, 846
Bse118I	RCCGGY	1 cut(s)	710
Bse1I	ACTGG	3 cut(s)	175, 361, 538
Bse8I	GATNNNNATC	1 cut(s)	338
BseBI	CCWGG	1 cut(s)	193
BseGI	GGATG	2 cut(s)	105, 339
BseJI	GATNNNNATC	1 cut(s)	338
BseLI	CCNNNNNNNGG	2 cut(s)	330, 846
BseMII	CTCAG	1 cut(s)	684
BseNI	ACTGG	3 cut(s)	175, 361, 538
BseRI	GAGGAG	1 cut(s)	747
BseXI	GCAGC	5 cut(s)	364, 383, 538, 803, 806
BsgI	GTGCAG	1 cut(s)	363
BshFI	GGCC	1 cut(s)	360
BshNI	GGYRCC	2 cut(s)	118, 813
BsiSI	CCGG	1 cut(s)	711
BslI	CCNNNNNNNGG	2 cut(s)	330, 846
BsmAI	GTCTC	2 cut(s)	207, 290
BsnI	GGCC	1 cut(s)	360
Bsp143I	GATC	2 cut(s)	481, 573
BspACI	CCGC	2 cut(s)	163, 586
BspANI	GGCC	1 cut(s)	360
BspCNI	CTCAG	1 cut(s)	685
BspHI	TCATGA	1 cut(s)	592
BspLI	GGNNCC	5 cut(s)	120, 135, 536, 575, 815
BspPI	GGATC	2 cut(s)	568, 581
BspT107I	GGYRCC	2 cut(s)	118, 813
BsrFI	RCCGGY	1 cut(s)	710
BsrI	ACTGG	3 cut(s)	175, 361, 538
BssAI	RCCGGY	1 cut(s)	710
BssMI	GATC	2 cut(s)	481, 573
Bst2UI	CCWGG	1 cut(s)	193
Bst4CI	ACNGT	2 cut(s)	25, 623
Bst6I	CTCTTC	1 cut(s)	522
BstC8I	GCNNGC	3 cut(s)	60, 160, 852
BstDEI	CTNAG	1 cut(s)	693
BstF5I	GGATG	2 cut(s)	105, 339
BstH2I	RGCGCY	2 cut(s)	289, 586
BstHHI	GCGC	3 cut(s)	198, 288, 585
BstKTI	GATC	2 cut(s)	484, 576
BstMAI	GTCTC	2 cut(s)	207, 290
BstMBI	GATC	2 cut(s)	481, 573
BstMWI	GCNNNNNNNGC	1 cut(s)	532
BstNI	CCWGG	1 cut(s)	193
BstNSI	RCATGY	1 cut(s)	131
BstSCI	CCNGG	1 cut(s)	191
BstV1I	GCAGC	5 cut(s)	364, 383, 538, 803, 806
BstX2I	RGATCY	2 cut(s)	481, 573
BstXI	CCANNNNNNTGG	1 cut(s)	545
BstYI	RGATCY	2 cut(s)	481, 573
BsuRI	GGCC	1 cut(s)	360
BtsCI	GGATG	2 cut(s)	105, 339
BtsI	GCAGTG	1 cut(s)	324
BtsIMutI	CAGTG	3 cut(s)	324, 545, 628
Cac8I	GCNNGC	3 cut(s)	60, 160, 852
CciI	TCATGA	1 cut(s)	592
CfoI	GCGC	3 cut(s)	198, 288, 585
Cfr10I	RCCGGY	1 cut(s)	710
Cfr13I	GGNCC	1 cut(s)	133
Csp6I	GTAC	1 cut(s)	814
CspCI	CAANNNNNGTGG	2 cut(s)	111, 146
CviAII	CATG	3 cut(s)	128, 593, 650
CviJI	RGCY	9 cut(s)	62, 162, 321, 360, 377, 443, 526, 535, 606
CviKI_1	RGCY	9 cut(s)	62, 162, 321, 360, 377, 443, 526, 535, 606
CviQI	GTAC	1 cut(s)	814
DdeI	CTNAG	1 cut(s)	693
DpnI	GATC	2 cut(s)	483, 575
DpnII	GATC	2 cut(s)	481, 573
DraI	TTTAAA	1 cut(s)	664
Eam1104I	CTCTTC	1 cut(s)	522
EarI	CTCTTC	1 cut(s)	522
Eco47I	GGWCC	1 cut(s)	133
Eco57I	CTGAAG	1 cut(s)	542
EcoRII	CCWGG	1 cut(s)	191
EcoT22I	ATGCAT	2 cut(s)	109, 129
FaeI	CATG	3 cut(s)	131, 596, 653
FatI	CATG	3 cut(s)	127, 592, 649
Fnu4HI	GCNGC	7 cut(s)	163, 378, 397, 552, 586, 792, 795
FokI	GGATG	2 cut(s)	112, 346
Fsp4HI	GCNGC	7 cut(s)	163, 378, 397, 552, 586, 792, 795
FspBI	CTAG	1 cut(s)	726
GlaI	GCGC	3 cut(s)	197, 287, 584
GluI	GCNGC	7 cut(s)	163, 378, 397, 552, 586, 792, 795
GsuI	CTGGAG	3 cut(s)	192, 431, 521
HaeII	RGCGCY	2 cut(s)	289, 586
HaeIII	GGCC	1 cut(s)	360
HapII	CCGG	1 cut(s)	711
HhaI	GCGC	3 cut(s)	198, 288, 585
Hin1II	CATG	3 cut(s)	131, 596, 653
Hin6I	GCGC	3 cut(s)	196, 286, 583
HinP1I	GCGC	3 cut(s)	196, 286, 583
HindIII	AAGCTT	1 cut(s)	524
HinfI	GANTC	3 cut(s)	18, 494, 643
HpaII	CCGG	1 cut(s)	711
Hpy166II	GTNNAC	4 cut(s)	72, 133, 179, 626
Hpy188I	TCNGA	2 cut(s)	354, 522
Hpy188III	TCNNGA	3 cut(s)	410, 485, 593
Hpy8I	GTNNAC	4 cut(s)	72, 133, 179, 626
HpyAV	CCTTC	1 cut(s)	827
HpyCH4III	ACNGT	2 cut(s)	25, 623
HpyCH4V	TGCA	7 cut(s)	107, 127, 329, 337, 380, 399, 791
HpyF10VI	GCNNNNNNNGC	1 cut(s)	532
HpyF3I	CTNAG	1 cut(s)	693
Hsp92II	CATG	3 cut(s)	131, 596, 653
HspAI	GCGC	3 cut(s)	196, 286, 583
KpnI	GGTACC	1 cut(s)	817
Kzo9I	GATC	2 cut(s)	481, 573
LmnI	GCTCC	3 cut(s)	326, 540, 734
Lsp1109I	GCAGC	5 cut(s)	364, 383, 538, 803, 806
LweI	GCATC	1 cut(s)	324
MaeI	CTAG	1 cut(s)	726
MaeIII	GTNAC	4 cut(s)	88, 387, 753, 833
MalI	GATC	2 cut(s)	483, 575
MboI	GATC	2 cut(s)	481, 573
MboII	GAAGA	4 cut(s)	31, 478, 491, 509
MflI	RGATCY	2 cut(s)	481, 573
MluCI	AATT	5 cut(s)	403, 446, 503, 597, 666
MmeI	TCCRAC	1 cut(s)	551
MnlI	CCTC	7 cut(s)	39, 167, 371, 406, 657, 725, 752
Mph1103I	ATGCAT	2 cut(s)	109, 129
MseI	TTAA	2 cut(s)	432, 663
MslI	CAYNNNNRTG	2 cut(s)	51, 808
MspA1I	CMGCKG	2 cut(s)	377, 588
MspI	CCGG	1 cut(s)	711
MspR9I	CCNGG	1 cut(s)	193
MvaI	CCWGG	1 cut(s)	193
MwoI	GCNNNNNNNGC	1 cut(s)	532
NdeII	GATC	2 cut(s)	481, 573
NlaIII	CATG	3 cut(s)	131, 596, 653
NlaIV	GGNNCC	5 cut(s)	120, 135, 536, 575, 815
NmuCI	GTSAC	1 cut(s)	387
NsiI	ATGCAT	2 cut(s)	109, 129
NspI	RCATGY	1 cut(s)	131
OliI	CACNNNNGTG	1 cut(s)	808
PagI	TCATGA	1 cut(s)	592
PfeI	GAWTC	3 cut(s)	18, 494, 643
PkrI	GCNGC	7 cut(s)	164, 379, 398, 553, 587, 793, 796
Psp6I	CCWGG	1 cut(s)	191
PspGI	CCWGG	1 cut(s)	191
PspN4I	GGNNCC	5 cut(s)	120, 135, 536, 575, 815
PspPI	GGNCC	1 cut(s)	133
PsuI	RGATCY	2 cut(s)	481, 573
PvuII	CAGCTG	1 cut(s)	377
RsaI	GTAC	1 cut(s)	815
RsaNI	GTAC	1 cut(s)	814
RseI	CAYNNNNRTG	2 cut(s)	51, 808
SaqAI	TTAA	2 cut(s)	432, 663
SatI	GCNGC	7 cut(s)	163, 378, 397, 552, 586, 792, 795
Sau3AI	GATC	2 cut(s)	481, 573
Sau96I	GGNCC	1 cut(s)	133
ScrFI	CCNGG	1 cut(s)	193
SetI	ASST	8 cut(s)	178, 323, 379, 417, 528, 744, 812, 819
SfaNI	GCATC	1 cut(s)	324
SinI	GGWCC	1 cut(s)	133
SmiMI	CAYNNNNRTG	2 cut(s)	51, 808
Sse9I	AATT	5 cut(s)	403, 446, 503, 597, 666
SsiI	CCGC	2 cut(s)	163, 586
SspMI	CTAG	1 cut(s)	726
StyD4I	CCNGG	1 cut(s)	191
TaaI	ACNGT	2 cut(s)	25, 623
TasI	AATT	5 cut(s)	403, 446, 503, 597, 666
TauI	GCSGC	2 cut(s)	165, 588
TfiI	GAWTC	3 cut(s)	18, 494, 643
Tru1I	TTAA	2 cut(s)	432, 663
Tru9I	TTAA	2 cut(s)	432, 663
TscAI	CASTG	3 cut(s)	331, 545, 628
TseFI	GTSAC	1 cut(s)	387
TseI	GCWGC	5 cut(s)	377, 396, 551, 791, 794
Tsp45I	GTSAC	1 cut(s)	387
TspDTI	ATGAA	1 cut(s)	609
TspGWI	ACGGA	1 cut(s)	418
TspRI	CASTG	3 cut(s)	331, 545, 628
VpaK11BI	GGWCC	1 cut(s)	133
XapI	RAATTY	3 cut(s)	446, 503, 597
XceI	RCATGY	1 cut(s)	131
XspI	CTAG	1 cut(s)	726
Zsp2I	ATGCAT	2 cut(s)	109, 129

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.