Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6B

Orthogroup ID

OG0002229

Physical Location & Seq

Genomic Coordinates Reverse (-)

26727397 .. 26730829

Gene Structure 3433 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6BG164900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 777 bp

ATGTATGCATATAAAATCGTGCCAATGTATGTGGACCCATTTCTTTATCCTACATTCGCCAGCCGCTTTACTGGAGGTTCACATATCATTTCTTGGCGCAAGTCTCCATCAGTTCCTATGGGTTATAGATATAGCGTCTGGCGTTCTGTCTTTTTGCTTTTGTTGATGGAAGTTCAAGTTGTGAAGCGGCTTATTGAGACAATATATGTATTCAAGTATAGCTCCACTGCTAGGATGCACATCTTTGGGTATCTGACTGGCCTCTTTTTCTACACAGCTGCACCCTTGTCACTTTGCTGCAATAATTTTCTGGAGGTCTATAACTTTTCCGTTAATGGAGTGGCTGAATTTATTGTCAAAGGCAAGAAGAATGCTATGCAAGATCTGGATTTTGATTCTTTGGAATTTATTAGTTCTTTTCTGAAGCTTGGTTGGCTCCAGTGGATTGGTGCTGCTTTATTCTTTTGGGGTTGGATCCATCAGCGCCGCTGTCATGAAATTCTTGGCTCATTACGAGAACACGGTGAACAAAATGTATATGTGATTCCTCATGGGGATTGGTTTAAAATTGTATCATCTCCACATTATCTTGCTGAGATAGTTATATATGCCGGTCTTGTGGTTGCTAGTGGAGGAGCAGACCTCACAATCTGGTTACTATTTGGATTTGTGGTATCAAACCTGGTGTTTGCAGCAGCAGAAACACATAGGTGGTACCTTCAAAAGTTCAAAAGTTACCCCAGAAATCGGCGTGCCATTATTCCATTTTTATACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003865 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0006066 GO:0006464 GO:0006486 GO:0006487 GO:0006629 GO:0006720 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0012505 GO:0016093 GO:0016229 GO:0016491 GO:0016627 GO:0019538 GO:0033765 GO:0034645 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0055114 GO:0070085 GO:0071704 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko00140 map00140

Pfam Domains

Protein Families

Steroid_dh

Protein Analysis

258

Amino Acids

29.98

Weight (kDa)

9.33

Isoelectric Point (pI)

43.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Steroid_dh	PF02544	147 - 258	1.5e-27	3-oxo-5-alpha-steroid 4-dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002229)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G72590 AT2G16530 AT2G16530 AT2G16530 AT2G16530 AT2G16530 AT3G43840 AT3G43840
fragaria_vesca	FvH4_2g10631 FvH4_2g10631 FvH4_2g10631 FvH4_2g10640 FvH4_2g10640 FvH4_2g10640
malus_domestica	MD05G1054700.v1.1 MD10G1062200.v1.1
prunus_persica	Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1
pyrus_communis	pycom05g04490 pycom05g04510 pycom10g04790
rosa_chinensis	RchiOBHm_Chr6g0268311 RchiOBHm_Chr6g0268321 RchiOBHm_Chr6g0268341
rosa_multiflora	Rmu_sc0009812.1_g000007 Rmu_sc0010202.1_g000011
rosa_roxburghii	Rroxscaffold_7G00199860 Rroxscaffold_7G00199880
rosa_rugosa	Rorug06G0040400 Rorug06G0040400 Rorug06G0040400
rosa_samantha	Rh6AG160500 Rh6BG164800 Rh6BG164900 Rh6CG153500 Rh6DG150600
rosa_wichuraiana	Rw0G000500 Rw6G013880 Rw6G013890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	714
AccB1I	GGYRCC	1 cut(s)	714
AciI	CCGC	3 cut(s)	64, 187, 487
AclWI	GGATC	2 cut(s)	469, 482
AcsI	RAATTY	3 cut(s)	347, 404, 498
AcuI	CTGAAG	1 cut(s)	443
AfaI	GTAC	1 cut(s)	716
AfiI	CCNNNNNNNGG	2 cut(s)	231, 747
AgsI	TTSAA	4 cut(s)	176, 214, 722, 730
AjnI	CCWGG	1 cut(s)	681
AleI	CACNNNNGTG	1 cut(s)	709
AluBI	AGCT	3 cut(s)	222, 278, 427
AluI	AGCT	3 cut(s)	222, 278, 427
Alw26I	GTCTC	2 cut(s)	108, 191
AlwI	GGATC	2 cut(s)	469, 482
AoxI	GGCC	1 cut(s)	259
ApeKI	GCWGC	5 cut(s)	278, 297, 452, 692, 695
ApoI	RAATTY	3 cut(s)	347, 404, 498
Asp718I	GGTACC	1 cut(s)	714
AspLEI	GCGC	2 cut(s)	99, 486
AspS9I	GGNCC	1 cut(s)	34
AsuHPI	GGTGA	1 cut(s)	536
AvaII	GGWCC	1 cut(s)	34
BamHI	GGATCC	1 cut(s)	474
BanI	GGYRCC	1 cut(s)	714
BbvI	GCAGC	5 cut(s)	265, 284, 439, 704, 707
BccI	CCATC	3 cut(s)	115, 160, 486
BciT130I	CCWGG	1 cut(s)	683
BcoDI	GTCTC	2 cut(s)	108, 191
BfaI	CTAG	2 cut(s)	231, 627
BfoI	RGCGCY	1 cut(s)	487
BglII	AGATCT	1 cut(s)	382
BisI	GCNGC	8 cut(s)	64, 188, 279, 298, 453, 487, 693, 696
BlsI	GCNGC	8 cut(s)	65, 189, 280, 299, 454, 488, 694, 697
Bme1390I	CCNGG	1 cut(s)	683
Bme18I	GGWCC	1 cut(s)	34
BmgT120I	GGNCC	1 cut(s)	34
BmiI	GGNNCC	4 cut(s)	36, 437, 476, 716
BmrFI	CCNGG	1 cut(s)	683
BmsI	GCATC	1 cut(s)	225
BplI	GAGNNNNNCTC	2 cut(s)	627, 659
BpmI	CTGGAG	3 cut(s)	93, 332, 422
BsaBI	GATNNNNATC	1 cut(s)	239
Bsc4I	CCNNNNNNNGG	2 cut(s)	231, 747
Bse118I	RCCGGY	1 cut(s)	611
Bse1I	ACTGG	3 cut(s)	76, 262, 439
Bse8I	GATNNNNATC	1 cut(s)	239
BseBI	CCWGG	1 cut(s)	683
BseGI	GGATG	1 cut(s)	240
BseJI	GATNNNNATC	1 cut(s)	239
BseLI	CCNNNNNNNGG	2 cut(s)	231, 747
BseMII	CTCAG	1 cut(s)	585
BseNI	ACTGG	3 cut(s)	76, 262, 439
BseRI	GAGGAG	1 cut(s)	648
BseXI	GCAGC	5 cut(s)	265, 284, 439, 704, 707
BsgI	GTGCAG	1 cut(s)	264
BshFI	GGCC	1 cut(s)	261
BshNI	GGYRCC	1 cut(s)	714
BsiSI	CCGG	1 cut(s)	612
BslI	CCNNNNNNNGG	2 cut(s)	231, 747
BsmAI	GTCTC	2 cut(s)	108, 191
BsmI	GAATGC	1 cut(s)	376
BsnI	GGCC	1 cut(s)	261
Bsp143I	GATC	2 cut(s)	382, 474
BspACI	CCGC	3 cut(s)	64, 187, 487
BspANI	GGCC	1 cut(s)	261
BspCNI	CTCAG	1 cut(s)	586
BspHI	TCATGA	1 cut(s)	493
BspLI	GGNNCC	4 cut(s)	36, 437, 476, 716
BspPI	GGATC	2 cut(s)	469, 482
BspT107I	GGYRCC	1 cut(s)	714
BsrFI	RCCGGY	1 cut(s)	611
BsrI	ACTGG	3 cut(s)	76, 262, 439
BssAI	RCCGGY	1 cut(s)	611
BssMI	GATC	2 cut(s)	382, 474
Bst2UI	CCWGG	1 cut(s)	683
Bst4CI	ACNGT	1 cut(s)	524
BstC8I	GCNNGC	2 cut(s)	61, 753
BstDEI	CTNAG	1 cut(s)	594
BstF5I	GGATG	1 cut(s)	240
BstH2I	RGCGCY	1 cut(s)	487
BstHHI	GCGC	2 cut(s)	99, 486
BstKTI	GATC	2 cut(s)	385, 477
BstMAI	GTCTC	2 cut(s)	108, 191
BstMBI	GATC	2 cut(s)	382, 474
BstMWI	GCNNNNNNNGC	1 cut(s)	433
BstNI	CCWGG	1 cut(s)	683
BstSCI	CCNGG	1 cut(s)	681
BstV1I	GCAGC	5 cut(s)	265, 284, 439, 704, 707
BstX2I	RGATCY	2 cut(s)	382, 474
BstXI	CCANNNNNNTGG	1 cut(s)	446
BstYI	RGATCY	2 cut(s)	382, 474
BsuRI	GGCC	1 cut(s)	261
BtsCI	GGATG	1 cut(s)	240
BtsI	GCAGTG	1 cut(s)	225
BtsIMutI	CAGTG	2 cut(s)	225, 446
Cac8I	GCNNGC	2 cut(s)	61, 753
CciI	TCATGA	1 cut(s)	493
CfoI	GCGC	2 cut(s)	99, 486
Cfr10I	RCCGGY	1 cut(s)	611
Cfr13I	GGNCC	1 cut(s)	34
CseI	GACGC	1 cut(s)	124
CsiI	ACCWGGT	1 cut(s)	681
Csp6I	GTAC	1 cut(s)	715
CspCI	CAANNNNNGTGG	2 cut(s)	12, 47
CviAII	CATG	2 cut(s)	494, 551
CviJI	RGCY	9 cut(s)	63, 190, 222, 261, 278, 344, 427, 436, 507
CviKI_1	RGCY	9 cut(s)	63, 190, 222, 261, 278, 344, 427, 436, 507
CviQI	GTAC	1 cut(s)	715
DdeI	CTNAG	1 cut(s)	594
DpnI	GATC	2 cut(s)	384, 476
DpnII	GATC	2 cut(s)	382, 474
DraI	TTTAAA	1 cut(s)	565
Eco47I	GGWCC	1 cut(s)	34
Eco57I	CTGAAG	1 cut(s)	443
EcoRII	CCWGG	1 cut(s)	681
EcoT22I	ATGCAT	1 cut(s)	10
FaeI	CATG	2 cut(s)	497, 554
FatI	CATG	2 cut(s)	493, 550
Fnu4HI	GCNGC	8 cut(s)	64, 188, 279, 298, 453, 487, 693, 696
FokI	GGATG	1 cut(s)	247
Fsp4HI	GCNGC	8 cut(s)	64, 188, 279, 298, 453, 487, 693, 696
FspBI	CTAG	2 cut(s)	231, 627
GlaI	GCGC	2 cut(s)	98, 485
GluI	GCNGC	8 cut(s)	64, 188, 279, 298, 453, 487, 693, 696
GsuI	CTGGAG	3 cut(s)	93, 332, 422
HaeII	RGCGCY	1 cut(s)	487
HaeIII	GGCC	1 cut(s)	261
HapII	CCGG	1 cut(s)	612
HgaI	GACGC	1 cut(s)	124
HhaI	GCGC	2 cut(s)	99, 486
Hin1II	CATG	2 cut(s)	497, 554
Hin6I	GCGC	2 cut(s)	97, 484
HinP1I	GCGC	2 cut(s)	97, 484
HindIII	AAGCTT	1 cut(s)	425
HinfI	GANTC	2 cut(s)	395, 544
HpaII	CCGG	1 cut(s)	612
HphI	GGTGA	1 cut(s)	536
Hpy166II	GTNNAC	3 cut(s)	34, 80, 527
Hpy188I	TCNGA	2 cut(s)	255, 423
Hpy188III	TCNNGA	3 cut(s)	311, 386, 494
Hpy8I	GTNNAC	3 cut(s)	34, 80, 527
HpyAV	CCTTC	1 cut(s)	728
HpyCH4III	ACNGT	1 cut(s)	524
HpyCH4V	TGCA	6 cut(s)	8, 238, 281, 300, 379, 692
HpyF10VI	GCNNNNNNNGC	1 cut(s)	433
HpyF3I	CTNAG	1 cut(s)	594
Hsp92II	CATG	2 cut(s)	497, 554
HspAI	GCGC	2 cut(s)	97, 484
KpnI	GGTACC	1 cut(s)	718
Kzo9I	GATC	2 cut(s)	382, 474
LmnI	GCTCC	3 cut(s)	227, 441, 635
Lsp1109I	GCAGC	5 cut(s)	265, 284, 439, 704, 707
LweI	GCATC	1 cut(s)	225
MabI	ACCWGGT	1 cut(s)	681
MaeI	CTAG	2 cut(s)	231, 627
MaeIII	GTNAC	3 cut(s)	288, 654, 734
MalI	GATC	2 cut(s)	384, 476
MboI	GATC	2 cut(s)	382, 474
MboII	GAAGA	1 cut(s)	379
MflI	RGATCY	2 cut(s)	382, 474
MluCI	AATT	5 cut(s)	304, 347, 404, 498, 567
MmeI	TCCRAC	1 cut(s)	452
MnlI	CCTC	6 cut(s)	68, 272, 307, 558, 626, 653
Mph1103I	ATGCAT	1 cut(s)	10
MseI	TTAA	2 cut(s)	333, 564
MslI	CAYNNNNRTG	1 cut(s)	709
MspA1I	CMGCKG	2 cut(s)	278, 489
MspI	CCGG	1 cut(s)	612
MspR9I	CCNGG	1 cut(s)	683
Mva1269I	GAATGC	1 cut(s)	376
MvaI	CCWGG	1 cut(s)	683
MwoI	GCNNNNNNNGC	1 cut(s)	433
NdeII	GATC	2 cut(s)	382, 474
NlaIII	CATG	2 cut(s)	497, 554
NlaIV	GGNNCC	4 cut(s)	36, 437, 476, 716
NmuCI	GTSAC	1 cut(s)	288
NsiI	ATGCAT	1 cut(s)	10
OliI	CACNNNNGTG	1 cut(s)	709
PagI	TCATGA	1 cut(s)	493
PctI	GAATGC	1 cut(s)	376
PfeI	GAWTC	2 cut(s)	395, 544
PkrI	GCNGC	8 cut(s)	65, 189, 280, 299, 454, 488, 694, 697
Psp6I	CCWGG	1 cut(s)	681
PspGI	CCWGG	1 cut(s)	681
PspN4I	GGNNCC	4 cut(s)	36, 437, 476, 716
PspPI	GGNCC	1 cut(s)	34
PsuI	RGATCY	2 cut(s)	382, 474
PvuII	CAGCTG	1 cut(s)	278
RsaI	GTAC	1 cut(s)	716
RsaNI	GTAC	1 cut(s)	715
RseI	CAYNNNNRTG	1 cut(s)	709
SaqAI	TTAA	2 cut(s)	333, 564
SatI	GCNGC	8 cut(s)	64, 188, 279, 298, 453, 487, 693, 696
Sau3AI	GATC	2 cut(s)	382, 474
Sau96I	GGNCC	1 cut(s)	34
ScrFI	CCNGG	1 cut(s)	683
SetI	ASST	9 cut(s)	79, 224, 280, 318, 429, 645, 684, 713, 720
SexAI	ACCWGGT	1 cut(s)	681
SfaNI	GCATC	1 cut(s)	225
SinI	GGWCC	1 cut(s)	34
SmiMI	CAYNNNNRTG	1 cut(s)	709
Sse9I	AATT	5 cut(s)	304, 347, 404, 498, 567
SsiI	CCGC	3 cut(s)	64, 187, 487
SspMI	CTAG	2 cut(s)	231, 627
StyD4I	CCNGG	1 cut(s)	681
TaaI	ACNGT	1 cut(s)	524
TasI	AATT	5 cut(s)	304, 347, 404, 498, 567
TauI	GCSGC	3 cut(s)	66, 190, 489
TfiI	GAWTC	2 cut(s)	395, 544
Tru1I	TTAA	2 cut(s)	333, 564
Tru9I	TTAA	2 cut(s)	333, 564
TscAI	CASTG	2 cut(s)	232, 446
TseFI	GTSAC	1 cut(s)	288
TseI	GCWGC	5 cut(s)	278, 297, 452, 692, 695
Tsp45I	GTSAC	1 cut(s)	288
TspDTI	ATGAA	1 cut(s)	510
TspGWI	ACGGA	1 cut(s)	319
TspRI	CASTG	2 cut(s)	232, 446
VpaK11BI	GGWCC	1 cut(s)	34
XapI	RAATTY	3 cut(s)	347, 404, 498
XspI	CTAG	2 cut(s)	231, 627
Zsp2I	ATGCAT	1 cut(s)	10

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.