Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6B

Orthogroup ID

OG0002229

Physical Location & Seq

Genomic Coordinates Reverse (-)

26721331 .. 26727059

Gene Structure 5729 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6BG164800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 585 bp

ATGCAATCTTCCTCGCAGAAATTCTCAGCTCCTCAGAAGTTCTTCAGTCATTTCTACATAGCGGCTGTTGTGTGGACAACTTTACTTCTTGTCCTGAGGCGCCTCTTTGAGACACTATACGTATTCAACTATAGCTCCTCGGCTAGGATGCACATTTTAGGATATCTGACTAGCTACATTTTCTACACAGTTGCACCCTTATCACTTTGCTGCAACCATGCTCTTGAGGTCTATAAATTTTCTCTAAATGGAGTGGCTGAGTTCCTTTTCAAAGGCAATACTAGTATGCTAGAATATGATCGGTTGGAATTCACAAGTCCTCTTTTGAAGCTTGGGTGGCTCCAATGGACTGGTGCAGCTATATTCTTTTGGGGTTGGATCCACCAGCGCAATTGTCATAAAATTCTTGGCTCATTACGGAAAAACAGAGAACAAAATGATGCGTATATGATCCCTTATGGTGATTGGTTTGAAATTGTTTCATCTCCACACTATCTTGCTGAGATAGTTATATATGTTGGTTTTGTGGTCGCTAGTGGAGGAGTAGACCTCACAATCTGGTTACTACTGGGATTCGTGGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003865 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0006066 GO:0006464 GO:0006486 GO:0006487 GO:0006629 GO:0006720 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0012505 GO:0016093 GO:0016229 GO:0016491 GO:0016627 GO:0019538 GO:0033765 GO:0034645 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0055114 GO:0070085 GO:0071704 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko00140 map00140

Pfam Domains

Protein Families

Steroid_dh

Protein Analysis

194

Amino Acids

22.43

Weight (kDa)

8.4

Isoelectric Point (pI)

46.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Steroid_dh	PF02544	115 - 190	2.8e-13	3-oxo-5-alpha-steroid 4-dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0002229)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G72590 AT2G16530 AT2G16530 AT2G16530 AT2G16530 AT2G16530 AT3G43840 AT3G43840
fragaria_vesca	FvH4_2g10631 FvH4_2g10631 FvH4_2g10631 FvH4_2g10640 FvH4_2g10640 FvH4_2g10640
malus_domestica	MD05G1054700.v1.1 MD10G1062200.v1.1
prunus_persica	Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1
pyrus_communis	pycom05g04490 pycom05g04510 pycom10g04790
rosa_chinensis	RchiOBHm_Chr6g0268311 RchiOBHm_Chr6g0268321 RchiOBHm_Chr6g0268341
rosa_multiflora	Rmu_sc0009812.1_g000007 Rmu_sc0010202.1_g000011
rosa_roxburghii	Rroxscaffold_7G00199860 Rroxscaffold_7G00199880
rosa_rugosa	Rorug06G0040400 Rorug06G0040400 Rorug06G0040400
rosa_samantha	Rh6AG160500 Rh6BG164800 Rh6BG164900 Rh6CG153500 Rh6DG150600
rosa_wichuraiana	Rw0G000500 Rw6G013880 Rw6G013890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	99
AccI	GTMKAC	1 cut(s)	546
AciI	CCGC	1 cut(s)	62
AclWI	GGATC	3 cut(s)	373, 386, 445
AcsI	RAATTY	4 cut(s)	20, 236, 308, 402
AcuI	CTGAAG	1 cut(s)	28
AcyI	GRCGYC	1 cut(s)	100
AfiI	CCNNNNNNNGG	1 cut(s)	144
AgsI	TTSAA	4 cut(s)	127, 271, 328, 473
AhlI	ACTAGT	1 cut(s)	281
AluBI	AGCT	5 cut(s)	29, 135, 174, 331, 359
AluI	AGCT	5 cut(s)	29, 135, 174, 331, 359
Alw26I	GTCTC	1 cut(s)	104
AlwI	GGATC	3 cut(s)	373, 386, 445
ApeKI	GCWGC	2 cut(s)	210, 356
ApoI	RAATTY	4 cut(s)	20, 236, 308, 402
Asp700I	GAANNNNTTC	1 cut(s)	41
AspLEI	GCGC	2 cut(s)	102, 390
AsuHPI	GGTGA	1 cut(s)	473
AxyI	CCTNAGG	1 cut(s)	95
BamHI	GGATCC	1 cut(s)	378
BanI	GGYRCC	1 cut(s)	99
BbvI	GCAGC	2 cut(s)	197, 368
BcoDI	GTCTC	1 cut(s)	104
BcuI	ACTAGT	1 cut(s)	281
BfaI	CTAG	5 cut(s)	144, 171, 282, 290, 534
BfmI	CTRYAG	1 cut(s)	130
BfoI	RGCGCY	1 cut(s)	103
BisI	GCNGC	3 cut(s)	63, 211, 357
BlsI	GCNGC	3 cut(s)	64, 212, 358
BmiI	GGNNCC	3 cut(s)	101, 341, 380
BmrI	ACTGGG	1 cut(s)	578
BmsI	GCATC	2 cut(s)	138, 430
BmuI	ACTGGG	1 cut(s)	578
BplI	GAGNNNNNCTC	2 cut(s)	534, 566
BpuEI	CTTGAG	1 cut(s)	245
BsaAI	YACGTR	1 cut(s)	121
BsaHI	GRCGYC	1 cut(s)	100
BsaJI	CCNNGG	1 cut(s)	138
BsaXI	ACNNNNNCTCC	2 cut(s)	119, 149
Bsc4I	CCNNNNNNNGG	1 cut(s)	144
Bse1I	ACTGG	2 cut(s)	355, 573
Bse21I	CCTNAGG	1 cut(s)	95
BseDI	CCNNGG	1 cut(s)	138
BseGI	GGATG	1 cut(s)	153
BseLI	CCNNNNNNNGG	1 cut(s)	144
BseMII	CTCAG	5 cut(s)	39, 47, 86, 249, 492
BseNI	ACTGG	2 cut(s)	355, 573
BseRI	GAGGAG	3 cut(s)	21, 127, 555
BseXI	GCAGC	2 cut(s)	197, 368
BsgI	GTGCAG	1 cut(s)	375
BshNI	GGYRCC	1 cut(s)	99
BslI	CCNNNNNNNGG	1 cut(s)	144
BsmAI	GTCTC	1 cut(s)	104
Bsp143I	GATC	3 cut(s)	298, 378, 450
BspACI	CCGC	1 cut(s)	62
BspCNI	CTCAG	5 cut(s)	38, 46, 87, 250, 493
BspLI	GGNNCC	3 cut(s)	101, 341, 380
BspPI	GGATC	3 cut(s)	373, 386, 445
BspT107I	GGYRCC	1 cut(s)	99
BsrI	ACTGG	2 cut(s)	355, 573
BssECI	CCNNGG	1 cut(s)	138
BssMI	GATC	3 cut(s)	298, 378, 450
BssNI	GRCGYC	1 cut(s)	100
Bst4CI	ACNGT	1 cut(s)	190
BstACI	GRCGYC	1 cut(s)	100
BstBAI	YACGTR	1 cut(s)	121
BstDEI	CTNAG	5 cut(s)	25, 33, 95, 258, 501
BstENI	CCTNNNNNAGG	1 cut(s)	142
BstF5I	GGATG	1 cut(s)	153
BstH2I	RGCGCY	1 cut(s)	103
BstHHI	GCGC	2 cut(s)	102, 390
BstKTI	GATC	3 cut(s)	301, 381, 453
BstMAI	GTCTC	1 cut(s)	104
BstMBI	GATC	3 cut(s)	298, 378, 450
BstMWI	GCNNNNNNNGC	1 cut(s)	337
BstSFI	CTRYAG	1 cut(s)	130
BstSNI	TACGTA	1 cut(s)	121
BstV1I	GCAGC	2 cut(s)	197, 368
BstX2I	RGATCY	1 cut(s)	378
BstXI	CCANNNNNNTGG	1 cut(s)	350
BstYI	RGATCY	1 cut(s)	378
Bsu36I	CCTNAGG	1 cut(s)	95
BtsCI	GGATG	1 cut(s)	153
CfoI	GCGC	2 cut(s)	102, 390
CviAII	CATG	1 cut(s)	218
DdeI	CTNAG	5 cut(s)	25, 33, 95, 258, 501
DinI	GGCGCC	1 cut(s)	101
DpnI	GATC	3 cut(s)	300, 380, 452
DpnII	GATC	3 cut(s)	298, 378, 450
Eco105I	TACGTA	1 cut(s)	121
Eco32I	GATATC	1 cut(s)	164
Eco57I	CTGAAG	1 cut(s)	28
Eco81I	CCTNAGG	1 cut(s)	95
EcoNI	CCTNNNNNAGG	1 cut(s)	142
EcoRI	GAATTC	1 cut(s)	308
EcoRV	GATATC	1 cut(s)	164
EgeI	GGCGCC	1 cut(s)	101
EheI	GGCGCC	1 cut(s)	101
FaeI	CATG	1 cut(s)	221
FatI	CATG	1 cut(s)	217
FblI	GTMKAC	1 cut(s)	546
Fnu4HI	GCNGC	3 cut(s)	63, 211, 357
FokI	GGATG	1 cut(s)	160
Fsp4HI	GCNGC	3 cut(s)	63, 211, 357
FspBI	CTAG	5 cut(s)	144, 171, 282, 290, 534
GlaI	GCGC	2 cut(s)	101, 389
GluI	GCNGC	3 cut(s)	63, 211, 357
HaeII	RGCGCY	1 cut(s)	103
HhaI	GCGC	2 cut(s)	102, 390
Hin1I	GRCGYC	1 cut(s)	100
Hin1II	CATG	1 cut(s)	221
Hin6I	GCGC	2 cut(s)	100, 388
HinP1I	GCGC	2 cut(s)	100, 388
HindIII	AAGCTT	1 cut(s)	329
HinfI	GANTC	1 cut(s)	573
HphI	GGTGA	1 cut(s)	473
Hpy166II	GTNNAC	2 cut(s)	75, 547
Hpy188I	TCNGA	2 cut(s)	36, 168
Hpy188III	TCNNGA	2 cut(s)	94, 224
Hpy8I	GTNNAC	2 cut(s)	75, 547
HpyCH4III	ACNGT	1 cut(s)	190
HpyCH4IV	ACGT	1 cut(s)	120
HpyCH4V	TGCA	5 cut(s)	4, 151, 194, 213, 356
HpyF10VI	GCNNNNNNNGC	1 cut(s)	337
HpyF3I	CTNAG	5 cut(s)	25, 33, 95, 258, 501
HpySE526I	ACGT	1 cut(s)	120
Hsp92I	GRCGYC	1 cut(s)	100
Hsp92II	CATG	1 cut(s)	221
HspAI	GCGC	2 cut(s)	100, 388
KasI	GGCGCC	1 cut(s)	99
Kzo9I	GATC	3 cut(s)	298, 378, 450
LmnI	GCTCC	3 cut(s)	34, 140, 345
LpnPI	CCDG	5 cut(s)	107, 336, 398, 544, 554
Lsp1109I	GCAGC	2 cut(s)	197, 368
LweI	GCATC	2 cut(s)	138, 430
MaeI	CTAG	5 cut(s)	144, 171, 282, 290, 534
MaeII	ACGT	1 cut(s)	120
MaeIII	GTNAC	1 cut(s)	561
MalI	GATC	3 cut(s)	300, 380, 452
MboI	GATC	3 cut(s)	298, 378, 450
MboII	GAAGA	1 cut(s)	34
MfeI	CAATTG	1 cut(s)	391
MflI	RGATCY	1 cut(s)	378
MluCI	AATT	6 cut(s)	20, 236, 308, 391, 402, 474
Mly113I	GGCGCC	1 cut(s)	100
MmeI	TCCRAC	2 cut(s)	285, 356
MnlI	CCTC	9 cut(s)	22, 42, 90, 113, 148, 220, 330, 533, 560
MroXI	GAANNNNTTC	1 cut(s)	41
MunI	CAATTG	1 cut(s)	391
MwoI	GCNNNNNNNGC	1 cut(s)	337
NarI	GGCGCC	1 cut(s)	100
NdeII	GATC	3 cut(s)	298, 378, 450
NlaIII	CATG	1 cut(s)	221
NlaIV	GGNNCC	3 cut(s)	101, 341, 380
NmeAIII	GCCGAG	1 cut(s)	119
PdmI	GAANNNNTTC	1 cut(s)	41
PfeI	GAWTC	1 cut(s)	573
PkrI	GCNGC	3 cut(s)	64, 212, 358
PluTI	GGCGCC	1 cut(s)	103
Ppu21I	YACGTR	1 cut(s)	121
PspN4I	GGNNCC	3 cut(s)	101, 341, 380
PsuI	RGATCY	1 cut(s)	378
SatI	GCNGC	3 cut(s)	63, 211, 357
Sau3AI	GATC	3 cut(s)	298, 378, 450
SetI	ASST	8 cut(s)	31, 123, 137, 176, 231, 333, 361, 552
SfaNI	GCATC	2 cut(s)	138, 430
SfcI	CTRYAG	1 cut(s)	130
SfoI	GGCGCC	1 cut(s)	101
SmlI	CTYRAG	1 cut(s)	224
SmoI	CTYRAG	1 cut(s)	224
SnaBI	TACGTA	1 cut(s)	121
SpeI	ACTAGT	1 cut(s)	281
Sse9I	AATT	6 cut(s)	20, 236, 308, 391, 402, 474
SsiI	CCGC	1 cut(s)	62
SspDI	GGCGCC	1 cut(s)	99
SspMI	CTAG	5 cut(s)	144, 171, 282, 290, 534
TaaI	ACNGT	1 cut(s)	190
TaiI	ACGT	1 cut(s)	123
TasI	AATT	6 cut(s)	20, 236, 308, 391, 402, 474
TauI	GCSGC	1 cut(s)	65
TfiI	GAWTC	1 cut(s)	573
TseI	GCWGC	2 cut(s)	210, 356
TspDTI	ATGAA	1 cut(s)	471
TspGWI	ACGGA	1 cut(s)	433
XagI	CCTNNNNNAGG	1 cut(s)	142
XapI	RAATTY	4 cut(s)	20, 236, 308, 402
XmiI	GTMKAC	1 cut(s)	546
XmnI	GAANNNNTTC	1 cut(s)	41
XspI	CTAG	5 cut(s)	144, 171, 282, 290, 534

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.