Rh6DG150600

polyprenol reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0002229
Physical Location & Seq
Reverse (-)
21782108 .. 21785316
3209 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG150600.1

Sequence Viewer

Length: 1014 bp
ATGGAGGTGGGGCTTGTTGCGCTGCTCAGAACAGCGTGGATTGCAGCGACATTGCCTTTACTCATAGCTTCGTTGCCCTTTTCTTGGCTCAACCCTGTTCATAAAACCGTTTTGGCGTTTGCCAAGAGAGGGAAGACCATGTCTTCTTCACGGAGATTCACAGTTCCTCAAAAGTTCTTCTGCCATTTCTATGTGCTGGCTGTTGTGTGGACAACCCTACTTCTTGTTACAACTTGGATGTATGCATATAAAATCGTGCCAATGTATGTGGACCCATTTCTTTATCCTACATTCGCCAGCCGCTTTACTGGAGGTTCACATATCATTTCTTGGCGCAAGTCTCCATCAGTTCCTATGGGTTATAGATATAGCGTCTGGCGTTCTGTCTTTTTGCTTTTGTTGATGGAAGTTCAAGTTGTGAAGCGGCTTATTGAGACAATATATGTATTCAAGTATAGCTCCACTGCTAGGATGCACATCTTTGGGTATCTGACTGGCCTCTTTTTCTACACAGCTGCACCCTTGTCACTTTGCTGCAATAATTTTCTGGAGGTCTATAACTTTTCCGTTAATGGAGTGGCTGAATTTATTGTCAAAGGCAAGAAGAATGCTATGCAAGATCTGGATTTTGATTCTTTGGAATTTATTAGTTCTTTTCTGAAGCTTGGTTGGCTCCAGTGGATTGGTGCTGCTTTATTCTTTTGGGGTTGGATCCATCAGCGCCGCTGTCATGAAATTCTTGGCTCATTACGAGAACACGGTGAACAAAATGTATATGTGATTCCTCATGGGGATTGGTTTAAAATTGTATCATCTCCACATTATCTTGCTGAGATAGTTATATATGCCGGTCTTGTGGTTGCTAGTGGAGGAGCAGACCTCACAATCTGGTTACTATTTGGATTTGTGGTATCAAACCTGGTGTTTGCAGCAGCAGAAACACATAGGTGGTACCTTCAAAAGTTCAAAAGTTACCCCAGAAATCGGCGTGCCATTATTCCATTTTTATACTAA

Protein Analysis

337

Amino Acids

38.92

Weight (kDa)

9.68

Isoelectric Point (pI)

46.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Steroid_dh PF02544 226 - 337 2e-27 3-oxo-5-alpha-steroid 4-dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 951
AccB1I GGYRCC 1 cut(s) 951
AciI CCGC 3 cut(s) 301, 424, 724
AclWI GGATC 2 cut(s) 706, 719
AcsI RAATTY 3 cut(s) 584, 641, 735
AcuI CTGAAG 1 cut(s) 680
AfaI GTAC 1 cut(s) 953
AfiI CCNNNNNNNGG 4 cut(s) 84, 129, 468, 984
AgsI TTSAA 4 cut(s) 413, 451, 959, 967
AjnI CCWGG 1 cut(s) 918
AleI CACNNNNGTG 1 cut(s) 946
AluBI AGCT 4 cut(s) 68, 459, 515, 664
AluI AGCT 4 cut(s) 68, 459, 515, 664
Alw26I GTCTC 2 cut(s) 345, 428
AlwI GGATC 2 cut(s) 706, 719
AoxI GGCC 1 cut(s) 496
ApeKI GCWGC 7 cut(s) 22, 44, 515, 534, 689, 929, 932
ApoI RAATTY 3 cut(s) 584, 641, 735
Asp718I GGTACC 1 cut(s) 951
AspLEI GCGC 3 cut(s) 22, 336, 723
AspS9I GGNCC 1 cut(s) 271
AsuHPI GGTGA 1 cut(s) 773
AvaII GGWCC 1 cut(s) 271
BamHI GGATCC 1 cut(s) 711
BanI GGYRCC 1 cut(s) 951
BbsI GAAGAC 2 cut(s) 135, 140
BbvI GCAGC 7 cut(s) 9, 56, 502, 521, 676, 941, 944
BccI CCATC 3 cut(s) 352, 397, 723
BciT130I CCWGG 1 cut(s) 920
BcoDI GTCTC 2 cut(s) 345, 428
BfaI CTAG 2 cut(s) 468, 864
BfoI RGCGCY 1 cut(s) 724
BglII AGATCT 1 cut(s) 619
Bme1390I CCNGG 1 cut(s) 920
Bme18I GGWCC 1 cut(s) 271
BmgT120I GGNCC 1 cut(s) 271
BmiI GGNNCC 4 cut(s) 273, 674, 713, 953
BmrFI CCNGG 1 cut(s) 920
BmsI GCATC 1 cut(s) 462
BpiI GAAGAC 2 cut(s) 135, 140
BplI GAGNNNNNCTC 2 cut(s) 864, 896
BpmI CTGGAG 3 cut(s) 330, 569, 659
BsaBI GATNNNNATC 1 cut(s) 476
Bsc4I CCNNNNNNNGG 4 cut(s) 84, 129, 468, 984
Bse118I RCCGGY 1 cut(s) 848
Bse1I ACTGG 3 cut(s) 313, 499, 676
Bse3DI GCAATG 1 cut(s) 50
Bse8I GATNNNNATC 1 cut(s) 476
BseBI CCWGG 1 cut(s) 920
BseGI GGATG 2 cut(s) 243, 477
BseJI GATNNNNATC 1 cut(s) 476
BseLI CCNNNNNNNGG 4 cut(s) 84, 129, 468, 984
BseMI GCAATG 1 cut(s) 50
BseMII CTCAG 2 cut(s) 40, 822
BseNI ACTGG 3 cut(s) 313, 499, 676
BseRI GAGGAG 1 cut(s) 885
BseXI GCAGC 7 cut(s) 9, 56, 502, 521, 676, 941, 944
BsgI GTGCAG 1 cut(s) 501
BshFI GGCC 1 cut(s) 498
BshNI GGYRCC 1 cut(s) 951
BsiSI CCGG 1 cut(s) 849
BslI CCNNNNNNNGG 4 cut(s) 84, 129, 468, 984
BsmAI GTCTC 2 cut(s) 345, 428
BsmI GAATGC 1 cut(s) 613
BsnI GGCC 1 cut(s) 498
Bsp143I GATC 2 cut(s) 619, 711
BspACI CCGC 3 cut(s) 301, 424, 724
BspANI GGCC 1 cut(s) 498
BspCNI CTCAG 2 cut(s) 39, 823
BspHI TCATGA 1 cut(s) 730
BspLI GGNNCC 4 cut(s) 273, 674, 713, 953
BspPI GGATC 2 cut(s) 706, 719
BspT107I GGYRCC 1 cut(s) 951
BsrDI GCAATG 1 cut(s) 50
BsrFI RCCGGY 1 cut(s) 848
BsrI ACTGG 3 cut(s) 313, 499, 676
BssAI RCCGGY 1 cut(s) 848
BssMI GATC 2 cut(s) 619, 711
Bst2UI CCWGG 1 cut(s) 920
Bst4CI ACNGT 3 cut(s) 109, 163, 761
BstC8I GCNNGC 3 cut(s) 198, 298, 990
BstDEI CTNAG 2 cut(s) 26, 831
BstF5I GGATG 2 cut(s) 243, 477
BstH2I RGCGCY 1 cut(s) 724
BstHHI GCGC 3 cut(s) 22, 336, 723
BstKTI GATC 2 cut(s) 622, 714
BstMAI GTCTC 2 cut(s) 345, 428
BstMBI GATC 2 cut(s) 619, 711
BstMWI GCNNNNNNNGC 3 cut(s) 19, 41, 670
BstNI CCWGG 1 cut(s) 920
BstSCI CCNGG 1 cut(s) 918
BstV1I GCAGC 7 cut(s) 9, 56, 502, 521, 676, 941, 944
BstV2I GAAGAC 2 cut(s) 135, 140
BstX2I RGATCY 2 cut(s) 619, 711
BstXI CCANNNNNNTGG 1 cut(s) 683
BstYI RGATCY 2 cut(s) 619, 711
BsuRI GGCC 1 cut(s) 498
BtsCI GGATG 2 cut(s) 243, 477
BtsI GCAGTG 1 cut(s) 462
BtsIMutI CAGTG 2 cut(s) 462, 683
Cac8I GCNNGC 3 cut(s) 198, 298, 990
CciI TCATGA 1 cut(s) 730
CfoI GCGC 3 cut(s) 22, 336, 723
Cfr10I RCCGGY 1 cut(s) 848
Cfr13I GGNCC 1 cut(s) 271
CseI GACGC 1 cut(s) 361
CsiI ACCWGGT 1 cut(s) 918
Csp6I GTAC 1 cut(s) 952
CspCI CAANNNNNGTGG 2 cut(s) 249, 284
CviAII CATG 3 cut(s) 139, 731, 788
CviQI GTAC 1 cut(s) 952
DdeI CTNAG 2 cut(s) 26, 831
DpnI GATC 2 cut(s) 621, 713
DpnII GATC 2 cut(s) 619, 711
DraI TTTAAA 1 cut(s) 802
Eco47I GGWCC 1 cut(s) 271
Eco57I CTGAAG 1 cut(s) 680
EcoRII CCWGG 1 cut(s) 918
EcoT22I ATGCAT 1 cut(s) 247
FaeI CATG 3 cut(s) 142, 734, 791
FatI CATG 3 cut(s) 138, 730, 787
FokI GGATG 2 cut(s) 250, 484
FspBI CTAG 2 cut(s) 468, 864
GlaI GCGC 3 cut(s) 21, 335, 722
GsuI CTGGAG 3 cut(s) 330, 569, 659
HaeII RGCGCY 1 cut(s) 724
HaeIII GGCC 1 cut(s) 498
HapII CCGG 1 cut(s) 849
HgaI GACGC 1 cut(s) 361
HhaI GCGC 3 cut(s) 22, 336, 723
Hin1II CATG 3 cut(s) 142, 734, 791
Hin6I GCGC 3 cut(s) 20, 334, 721
HinP1I GCGC 3 cut(s) 20, 334, 721
HindIII AAGCTT 1 cut(s) 662
HinfI GANTC 3 cut(s) 156, 632, 781
HpaII CCGG 1 cut(s) 849
HphI GGTGA 1 cut(s) 773
Hpy166II GTNNAC 4 cut(s) 210, 271, 317, 764
Hpy188I TCNGA 3 cut(s) 29, 492, 660
Hpy188III TCNNGA 3 cut(s) 548, 623, 731
Hpy8I GTNNAC 4 cut(s) 210, 271, 317, 764
HpyAV CCTTC 1 cut(s) 965
HpyCH4III ACNGT 3 cut(s) 109, 163, 761
HpyCH4V TGCA 7 cut(s) 44, 245, 475, 518, 537, 616, 929
HpyF10VI GCNNNNNNNGC 3 cut(s) 19, 41, 670
HpyF3I CTNAG 2 cut(s) 26, 831
Hsp92II CATG 3 cut(s) 142, 734, 791
HspAI GCGC 3 cut(s) 20, 334, 721
KpnI GGTACC 1 cut(s) 955
Kzo9I GATC 2 cut(s) 619, 711
LmnI GCTCC 3 cut(s) 464, 678, 872
Lsp1109I GCAGC 7 cut(s) 9, 56, 502, 521, 676, 941, 944
LweI GCATC 1 cut(s) 462
MabI ACCWGGT 1 cut(s) 918
MaeI CTAG 2 cut(s) 468, 864
MaeIII GTNAC 4 cut(s) 226, 525, 891, 971
MalI GATC 2 cut(s) 621, 713
MboI GATC 2 cut(s) 619, 711
MboII GAAGA 5 cut(s) 135, 138, 145, 169, 616
MflI RGATCY 2 cut(s) 619, 711
MluCI AATT 5 cut(s) 541, 584, 641, 735, 804
MmeI TCCRAC 1 cut(s) 689
MnlI CCTC 8 cut(s) 122, 177, 305, 509, 544, 795, 863, 890
Mph1103I ATGCAT 1 cut(s) 247
MseI TTAA 2 cut(s) 570, 801
MslI CAYNNNNRTG 2 cut(s) 189, 946
MspA1I CMGCKG 2 cut(s) 515, 726
MspI CCGG 1 cut(s) 849
MspR9I CCNGG 1 cut(s) 920
Mva1269I GAATGC 1 cut(s) 613
MvaI CCWGG 1 cut(s) 920
MwoI GCNNNNNNNGC 3 cut(s) 19, 41, 670
NdeII GATC 2 cut(s) 619, 711
NlaIII CATG 3 cut(s) 142, 734, 791
NlaIV GGNNCC 4 cut(s) 273, 674, 713, 953
NmuCI GTSAC 1 cut(s) 525
NsiI ATGCAT 1 cut(s) 247
OliI CACNNNNGTG 1 cut(s) 946
PagI TCATGA 1 cut(s) 730
PctI GAATGC 1 cut(s) 613
PfeI GAWTC 3 cut(s) 156, 632, 781
PflFI GACNNNGTC 1 cut(s) 139
Psp6I CCWGG 1 cut(s) 918
PspGI CCWGG 1 cut(s) 918
PspN4I GGNNCC 4 cut(s) 273, 674, 713, 953
PspPI GGNCC 1 cut(s) 271
PsuI RGATCY 2 cut(s) 619, 711
PsyI GACNNNGTC 1 cut(s) 139
PvuII CAGCTG 1 cut(s) 515
RsaI GTAC 1 cut(s) 953
RsaNI GTAC 1 cut(s) 952
RseI CAYNNNNRTG 2 cut(s) 189, 946
SaqAI TTAA 2 cut(s) 570, 801
Sau3AI GATC 2 cut(s) 619, 711
Sau96I GGNCC 1 cut(s) 271
ScrFI CCNGG 1 cut(s) 920
SexAI ACCWGGT 1 cut(s) 918
SfaNI GCATC 1 cut(s) 462
SinI GGWCC 1 cut(s) 271
SmiMI CAYNNNNRTG 2 cut(s) 189, 946
Sse9I AATT 5 cut(s) 541, 584, 641, 735, 804
SsiI CCGC 3 cut(s) 301, 424, 724
SspMI CTAG 2 cut(s) 468, 864
StyD4I CCNGG 1 cut(s) 918
TaaI ACNGT 3 cut(s) 109, 163, 761
TasI AATT 5 cut(s) 541, 584, 641, 735, 804
TauI GCSGC 3 cut(s) 303, 427, 726
TfiI GAWTC 3 cut(s) 156, 632, 781
Tru1I TTAA 2 cut(s) 570, 801
Tru9I TTAA 2 cut(s) 570, 801
TscAI CASTG 2 cut(s) 469, 683
TseFI GTSAC 1 cut(s) 525
TseI GCWGC 7 cut(s) 22, 44, 515, 534, 689, 929, 932
Tsp45I GTSAC 1 cut(s) 525
TspDTI ATGAA 2 cut(s) 89, 747
TspGWI ACGGA 2 cut(s) 166, 556
TspRI CASTG 2 cut(s) 469, 683
Tth111I GACNNNGTC 1 cut(s) 139
VpaK11BI GGWCC 1 cut(s) 271
XapI RAATTY 3 cut(s) 584, 641, 735
XspI CTAG 2 cut(s) 468, 864
Zsp2I ATGCAT 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.