Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0002229

Physical Location & Seq

Genomic Coordinates Reverse (-)

25656271 .. 25658319

Gene Structure 2049 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G013880.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 939 bp

ATGGAGATGGGGCTTGTTGGGTTGCTGAGAATAGCTTGGATTGCATCCACACTGCCTTTTCTCTTAGCTTCTGTACCCTGTTCAGGGCTGAACTGGCTTTATGAACGTCTTATGGTATTTGGCAAGCGAGGGAAAATCATGCAATCTTCCTCGCAGAAATTCTCAGTTCCTCAGAAGTTCTTCATTCATTTCTACATAGCGGCTGTTGTGTGGACAGCTTTACTTCTTGTTACGACTTGGATGTATGCATGTAATGTGGTGCCATTCGAGAATCACTCTACTGGAGGTTCACATATCTTTAGTGTTTGGCGTTCAGTGTTTCTGCTTTTATTGATGGAAGTTCAGGTCCTGAGGCGCATCTTTGAGACACTATACGTATTCAACTATAGTTCCTCGGCTAGGATGCACATTTTAGGATATCTGACTAGCTACATTTACTACACAGTTGCACCCTTATCACTTTGCTGCAACCATGCTCTTGAGGTCTATAAATTTTCTCTAAAAGGAGTGGCTGAGTTCCTTTTCAAAGGCAATACTAGTATGCTAGAATATGATCGGTTGGAATTCACAAGTCCTCTTTTGAAGCTTGGGTGGCTCCAATGGACTGGTGCAGCTATATTCTTTTGGGGTTGGATCCACCAGCGCAATTGTCATAAAATTCTTGGCTCATTACGGAAAAATAGAGAACAAAATGATGCGTATATGATCCCTTATGGTGATTGGTTTGGAATTGTTTCATCTCCACACTATCTTGCTGAGATAGTTATATATGTTGGTTTTGTGGTCGCTAGTGGAGGAGTAGACCTCACAATCTGGTTACTACTGGGATTCGTGGTGGTTAACTTAACGTTTACAGCAGCGATTACACATAGGTGGTACCATCGGAAATTCGAAAATTACCCCAGCAATCGGTATGCCATTATTCCACTTGTATACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003865 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0006066 GO:0006464 GO:0006486 GO:0006487 GO:0006629 GO:0006720 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0012505 GO:0016093 GO:0016229 GO:0016491 GO:0016627 GO:0019538 GO:0033765 GO:0034645 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0055114 GO:0070085 GO:0071704 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko00140 map00140

Pfam Domains

Protein Families

Steroid_dh

Protein Analysis

312

Amino Acids

36.07

Weight (kDa)

9.27

Isoelectric Point (pI)

43.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Steroid_dh	PF02544	200 - 312	4.5e-27	3-oxo-5-alpha-steroid 4-dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002229)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G72590 AT2G16530 AT2G16530 AT2G16530 AT2G16530 AT2G16530 AT3G43840 AT3G43840
fragaria_vesca	FvH4_2g10631 FvH4_2g10631 FvH4_2g10631 FvH4_2g10640 FvH4_2g10640 FvH4_2g10640
malus_domestica	MD05G1054700.v1.1 MD10G1062200.v1.1
prunus_persica	Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1
pyrus_communis	pycom05g04490 pycom05g04510 pycom10g04790
rosa_chinensis	RchiOBHm_Chr6g0268311 RchiOBHm_Chr6g0268321 RchiOBHm_Chr6g0268341
rosa_multiflora	Rmu_sc0009812.1_g000007 Rmu_sc0010202.1_g000011
rosa_roxburghii	Rroxscaffold_7G00199860 Rroxscaffold_7G00199880
rosa_rugosa	Rorug06G0040400 Rorug06G0040400 Rorug06G0040400
rosa_samantha	Rh6AG160500 Rh6BG164800 Rh6BG164900 Rh6CG153500 Rh6DG150600
rosa_wichuraiana	Rw0G000500 Rw6G013880 Rw6G013890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	876
AccB1I	GGYRCC	2 cut(s)	259, 876
AccI	GTMKAC	2 cut(s)	801, 933
AciI	CCGC	1 cut(s)	200
AclI	AACGTT	1 cut(s)	848
AclWI	GGATC	3 cut(s)	628, 641, 700
AcsI	RAATTY	5 cut(s)	158, 491, 563, 657, 887
AfaI	GTAC	2 cut(s)	75, 878
AfiI	CCNNNNNNNGG	4 cut(s)	83, 84, 399, 909
AgsI	TTSAA	3 cut(s)	382, 526, 583
AhlI	ACTAGT	1 cut(s)	536
AleI	CACNNNNGTG	1 cut(s)	871
AluBI	AGCT	6 cut(s)	35, 68, 218, 429, 586, 614
AluI	AGCT	6 cut(s)	35, 68, 218, 429, 586, 614
Alw26I	GTCTC	1 cut(s)	359
AlwI	GGATC	3 cut(s)	628, 641, 700
AlwNI	CAGNNNCTG	1 cut(s)	349
ApeKI	GCWGC	3 cut(s)	465, 611, 857
ApoI	RAATTY	5 cut(s)	158, 491, 563, 657, 887
Asp700I	GAANNNNTTC	2 cut(s)	179, 733
Asp718I	GGTACC	1 cut(s)	876
AspLEI	GCGC	2 cut(s)	357, 645
AspS9I	GGNCC	1 cut(s)	346
AsuHPI	GGTGA	1 cut(s)	728
AsuII	TTCGAA	1 cut(s)	891
AvaII	GGWCC	1 cut(s)	346
AxyI	CCTNAGG	1 cut(s)	350
BamHI	GGATCC	1 cut(s)	633
BanI	GGYRCC	2 cut(s)	259, 876
BbvI	GCAGC	3 cut(s)	452, 623, 869
BccI	CCATC	2 cut(s)	328, 888
BcoDI	GTCTC	1 cut(s)	359
BcuI	ACTAGT	1 cut(s)	536
BfaI	CTAG	6 cut(s)	399, 426, 537, 545, 789, 937
BfmI	CTRYAG	1 cut(s)	385
BisI	GCNGC	4 cut(s)	201, 466, 612, 858
BlsI	GCNGC	4 cut(s)	202, 467, 613, 859
Bme18I	GGWCC	1 cut(s)	346
BmgT120I	GGNCC	1 cut(s)	346
BmiI	GGNNCC	4 cut(s)	261, 596, 635, 878
BmrI	ACTGGG	1 cut(s)	833
BmsI	GCATC	4 cut(s)	53, 366, 393, 685
BmuI	ACTGGG	1 cut(s)	833
BplI	GAGNNNNNCTC	4 cut(s)	260, 292, 789, 821
BpmI	CTGGAG	1 cut(s)	303
Bpu14I	TTCGAA	1 cut(s)	891
BpuEI	CTTGAG	1 cut(s)	500
BsaAI	YACGTR	1 cut(s)	376
BsaJI	CCNNGG	1 cut(s)	393
Bsc4I	CCNNNNNNNGG	4 cut(s)	83, 84, 399, 909
Bse1I	ACTGG	4 cut(s)	98, 286, 610, 828
Bse21I	CCTNAGG	1 cut(s)	350
BseDI	CCNNGG	1 cut(s)	393
BseGI	GGATG	3 cut(s)	44, 246, 408
BseLI	CCNNNNNNNGG	4 cut(s)	83, 84, 399, 909
BseMII	CTCAG	6 cut(s)	17, 177, 185, 341, 504, 747
BseNI	ACTGG	4 cut(s)	98, 286, 610, 828
BseRI	GAGGAG	1 cut(s)	810
BseXI	GCAGC	3 cut(s)	452, 623, 869
BseYI	CCCAGC	1 cut(s)	902
BsgI	GTGCAG	1 cut(s)	630
BshNI	GGYRCC	2 cut(s)	259, 876
BslI	CCNNNNNNNGG	4 cut(s)	83, 84, 399, 909
BsmAI	GTCTC	1 cut(s)	359
Bsp119I	TTCGAA	1 cut(s)	891
Bsp143I	GATC	3 cut(s)	553, 633, 705
BspACI	CCGC	1 cut(s)	200
BspCNI	CTCAG	6 cut(s)	18, 176, 184, 342, 505, 748
BspLI	GGNNCC	4 cut(s)	261, 596, 635, 878
BspPI	GGATC	3 cut(s)	628, 641, 700
BspT104I	TTCGAA	1 cut(s)	891
BspT107I	GGYRCC	2 cut(s)	259, 876
BsrI	ACTGG	4 cut(s)	98, 286, 610, 828
BssECI	CCNNGG	1 cut(s)	393
BssMI	GATC	3 cut(s)	553, 633, 705
BssNAI	GTATAC	1 cut(s)	934
Bst1107I	GTATAC	1 cut(s)	934
Bst4CI	ACNGT	1 cut(s)	445
BstBAI	YACGTR	1 cut(s)	376
BstBI	TTCGAA	1 cut(s)	891
BstC8I	GCNNGC	1 cut(s)	125
BstDEI	CTNAG	7 cut(s)	26, 64, 163, 171, 350, 513, 756
BstENI	CCTNNNNNAGG	1 cut(s)	397
BstF5I	GGATG	3 cut(s)	44, 246, 408
BstHHI	GCGC	2 cut(s)	357, 645
BstKTI	GATC	3 cut(s)	556, 636, 708
BstMAI	GTCTC	1 cut(s)	359
BstMBI	GATC	3 cut(s)	553, 633, 705
BstMWI	GCNNNNNNNGC	3 cut(s)	41, 94, 592
BstNSI	RCATGY	1 cut(s)	252
BstSFI	CTRYAG	1 cut(s)	385
BstSNI	TACGTA	1 cut(s)	376
BstV1I	GCAGC	3 cut(s)	452, 623, 869
BstX2I	RGATCY	1 cut(s)	633
BstXI	CCANNNNNNTGG	1 cut(s)	605
BstYI	RGATCY	1 cut(s)	633
BstZ17I	GTATAC	1 cut(s)	934
Bsu36I	CCTNAGG	1 cut(s)	350
BtsCI	GGATG	3 cut(s)	44, 246, 408
BtsI	GCAGTG	1 cut(s)	50
BtsIMutI	CAGTG	2 cut(s)	50, 321
Cac8I	GCNNGC	1 cut(s)	125
CaiI	CAGNNNCTG	1 cut(s)	349
CfoI	GCGC	2 cut(s)	357, 645
Cfr13I	GGNCC	1 cut(s)	346
Csp6I	GTAC	2 cut(s)	74, 877
CviAII	CATG	3 cut(s)	139, 249, 473
CviQI	GTAC	2 cut(s)	74, 877
DdeI	CTNAG	7 cut(s)	26, 64, 163, 171, 350, 513, 756
DpnI	GATC	3 cut(s)	555, 635, 707
DpnII	GATC	3 cut(s)	553, 633, 705
Eco105I	TACGTA	1 cut(s)	376
Eco32I	GATATC	1 cut(s)	419
Eco47I	GGWCC	1 cut(s)	346
Eco81I	CCTNAGG	1 cut(s)	350
EcoNI	CCTNNNNNAGG	1 cut(s)	397
EcoO109I	RGGNCCY	1 cut(s)	346
EcoRI	GAATTC	1 cut(s)	563
EcoRV	GATATC	1 cut(s)	419
EcoT22I	ATGCAT	1 cut(s)	250
FaeI	CATG	3 cut(s)	142, 252, 476
FatI	CATG	3 cut(s)	138, 248, 472
FblI	GTMKAC	2 cut(s)	801, 933
Fnu4HI	GCNGC	4 cut(s)	201, 466, 612, 858
FokI	GGATG	3 cut(s)	31, 253, 415
Fsp4HI	GCNGC	4 cut(s)	201, 466, 612, 858
FspBI	CTAG	6 cut(s)	399, 426, 537, 545, 789, 937
GlaI	GCGC	2 cut(s)	356, 644
GluI	GCNGC	4 cut(s)	201, 466, 612, 858
GsaI	CCCAGC	1 cut(s)	906
GsuI	CTGGAG	1 cut(s)	303
HhaI	GCGC	2 cut(s)	357, 645
Hin1II	CATG	3 cut(s)	142, 252, 476
Hin6I	GCGC	2 cut(s)	355, 643
HinP1I	GCGC	2 cut(s)	355, 643
HincII	GTYRAC	1 cut(s)	841
HindII	GTYRAC	1 cut(s)	841
HindIII	AAGCTT	1 cut(s)	584
HinfI	GANTC	2 cut(s)	271, 828
HpaI	GTTAAC	1 cut(s)	841
HphI	GGTGA	1 cut(s)	728
Hpy166II	GTNNAC	6 cut(s)	213, 290, 802, 841, 852, 934
Hpy188I	TCNGA	3 cut(s)	174, 423, 885
Hpy188III	TCNNGA	3 cut(s)	268, 349, 479
Hpy8I	GTNNAC	6 cut(s)	213, 290, 802, 841, 852, 934
HpyCH4III	ACNGT	1 cut(s)	445
HpyCH4IV	ACGT	3 cut(s)	106, 375, 848
HpyCH4V	TGCA	7 cut(s)	44, 142, 248, 406, 449, 468, 611
HpyF10VI	GCNNNNNNNGC	3 cut(s)	41, 94, 592
HpyF3I	CTNAG	7 cut(s)	26, 64, 163, 171, 350, 513, 756
HpySE526I	ACGT	3 cut(s)	106, 375, 848
Hsp92II	CATG	3 cut(s)	142, 252, 476
HspAI	GCGC	2 cut(s)	355, 643
KpnI	GGTACC	1 cut(s)	880
KspAI	GTTAAC	1 cut(s)	841
Kzo9I	GATC	3 cut(s)	553, 633, 705
LmnI	GCTCC	1 cut(s)	600
Lsp1109I	GCAGC	3 cut(s)	452, 623, 869
LweI	GCATC	4 cut(s)	53, 366, 393, 685
MaeI	CTAG	6 cut(s)	399, 426, 537, 545, 789, 937
MaeII	ACGT	3 cut(s)	106, 375, 848
MaeIII	GTNAC	2 cut(s)	229, 816
MalI	GATC	3 cut(s)	555, 635, 707
MboI	GATC	3 cut(s)	553, 633, 705
MboII	GAAGA	2 cut(s)	138, 172
MfeI	CAATTG	1 cut(s)	646
MflI	RGATCY	1 cut(s)	633
MluCI	AATT	8 cut(s)	158, 491, 563, 646, 657, 729, 887, 895
MmeI	TCCRAC	2 cut(s)	540, 611
Mph1103I	ATGCAT	1 cut(s)	250
MroXI	GAANNNNTTC	2 cut(s)	179, 733
MseI	TTAA	2 cut(s)	840, 845
MslI	CAYNNNNRTG	1 cut(s)	871
MunI	CAATTG	1 cut(s)	646
MwoI	GCNNNNNNNGC	3 cut(s)	41, 94, 592
NdeII	GATC	3 cut(s)	553, 633, 705
NlaIII	CATG	3 cut(s)	142, 252, 476
NlaIV	GGNNCC	4 cut(s)	261, 596, 635, 878
NmeAIII	GCCGAG	1 cut(s)	374
NsiI	ATGCAT	1 cut(s)	250
NspI	RCATGY	1 cut(s)	252
NspV	TTCGAA	1 cut(s)	891
OliI	CACNNNNGTG	1 cut(s)	871
PdmI	GAANNNNTTC	2 cut(s)	179, 733
PfeI	GAWTC	2 cut(s)	271, 828
PkrI	GCNGC	4 cut(s)	202, 467, 613, 859
Ppu21I	YACGTR	1 cut(s)	376
PpuMI	RGGWCCY	1 cut(s)	346
Psp1406I	AACGTT	1 cut(s)	848
Psp5II	RGGWCCY	1 cut(s)	346
PspFI	CCCAGC	1 cut(s)	902
PspN4I	GGNNCC	4 cut(s)	261, 596, 635, 878
PspPI	GGNCC	1 cut(s)	346
PspPPI	RGGWCCY	1 cut(s)	346
PstNI	CAGNNNCTG	1 cut(s)	349
PsuI	RGATCY	1 cut(s)	633
RsaI	GTAC	2 cut(s)	75, 878
RsaNI	GTAC	2 cut(s)	74, 877
RseI	CAYNNNNRTG	1 cut(s)	871
SaqAI	TTAA	2 cut(s)	840, 845
SatI	GCNGC	4 cut(s)	201, 466, 612, 858
Sau3AI	GATC	3 cut(s)	553, 633, 705
Sau96I	GGNCC	1 cut(s)	346
SfaNI	GCATC	4 cut(s)	53, 366, 393, 685
SfcI	CTRYAG	1 cut(s)	385
SfuI	TTCGAA	1 cut(s)	891
SinI	GGWCC	1 cut(s)	346
SmiMI	CAYNNNNRTG	1 cut(s)	871
SmlI	CTYRAG	1 cut(s)	479
SmoI	CTYRAG	1 cut(s)	479
SnaBI	TACGTA	1 cut(s)	376
SpeI	ACTAGT	1 cut(s)	536
Sse9I	AATT	8 cut(s)	158, 491, 563, 646, 657, 729, 887, 895
SsiI	CCGC	1 cut(s)	200
SspMI	CTAG	6 cut(s)	399, 426, 537, 545, 789, 937
TaaI	ACNGT	1 cut(s)	445
TaiI	ACGT	3 cut(s)	109, 378, 851
TaqI	TCGA	2 cut(s)	267, 891
TasI	AATT	8 cut(s)	158, 491, 563, 646, 657, 729, 887, 895
TauI	GCSGC	1 cut(s)	203
TfiI	GAWTC	2 cut(s)	271, 828
Tru1I	TTAA	2 cut(s)	840, 845
Tru9I	TTAA	2 cut(s)	840, 845
TscAI	CASTG	2 cut(s)	57, 321
TseI	GCWGC	3 cut(s)	465, 611, 857
TspDTI	ATGAA	4 cut(s)	117, 172, 176, 726
TspGWI	ACGGA	1 cut(s)	688
TspRI	CASTG	2 cut(s)	57, 321
VpaK11BI	GGWCC	1 cut(s)	346
XagI	CCTNNNNNAGG	1 cut(s)	397
XapI	RAATTY	5 cut(s)	158, 491, 563, 657, 887
XceI	RCATGY	1 cut(s)	252
XmiI	GTMKAC	2 cut(s)	801, 933
XmnI	GAANNNNTTC	2 cut(s)	179, 733
XspI	CTAG	6 cut(s)	399, 426, 537, 545, 789, 937
Zsp2I	ATGCAT	1 cut(s)	250

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.