Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0002229

Physical Location & Seq

Genomic Coordinates Forward (+)

9385129 .. 9388096

Gene Structure 2968 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1054700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1080 bp

ATGTCACAGTTTCCAGCCTTTCCCAAATCATTAAGCTCTTTCCTGACTTGGGTATTCGCTCTAATGGAGGTGGGTGTTGTTGGGTTGCTTAGAACAGCATGGGTTGCGGCGACTATGCCCATACTCGTAGCCGCCGTACCTTGTTCTGGGCTGGCCTCGTTTGATGGAGCTGTTCTGGGGTTTGCCAAGAGAGGGAAGATCACGCAGTCGTCATCTCAGAAGTTCACAGTTCCTCAAAAGTTCTTCTGCCATTTCTATGTGGTGGCTGTAGCGTGGACAACGCTATTGCTCGTTGCAACTTCGATGTATGCGTATAAAACGGTGCCATTGGTTTCAGAATCGTTGCTTTACCCTTCAATCGCCAGCCACTTCACTGGGGGTTTACATATCGTTTCATGGAACAAGGTTCATTCGGTTCCTGTCAGTTATAGATATAGCATTTGGCGTTCGGTCTTTCTGCTTTTATTGATGGAAGTTCAAGTCTTGAGGCGCCTCTTTGAGACAATATATGTGTTCAACTATAGCTCCTCCGCGCGGATGCACATTTTCGGATATCTGACTGGCCTCTTCTTCTACACAGCAGCACCACTGTCACTTTGCTGCAATAATGTTCTGGAGGTCTATAAATTTTCAATAAGTGGAGTAGCTGAGTTCATTGTCAAAGGCAAGAGTAGCATGCGAGAAGTGGAATTTGATTGGTTACAATTTGTGAACTCTCTTTTGAAGCTTGGTTGGCTCCAGTGGATTGGTGCAGCTATATTTTTTTGGGGTTGGATCCATCAGCGCCGATGTCATGCAATTCTTGGCTCATTACGAGATCACAGTGAACCAAATGATGAATATGTGATTCCTCATGGTGATTGGTTTGAAATTGTTTCATCTCCACACTATCTGGCTGAGATGGTTATATATGCTGGCCTTGTGGTCGCTAGTGGAGGAACAGATCTCACAATCTGGTTACTTTTTGGATTTGTGGTGTCCAACTTAGTATTGGCAGCAGCGGAAACGCATAGGTGGTACCTCCAGAAATTTGAAAGCTATCCCAAGAAACGGCTCGCCATTATTCCATTTGTATACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003865 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0006066 GO:0006464 GO:0006486 GO:0006487 GO:0006629 GO:0006720 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0012505 GO:0016093 GO:0016229 GO:0016491 GO:0016627 GO:0019538 GO:0033765 GO:0034645 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0055114 GO:0070085 GO:0071704 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko00140 map00140

Pfam Domains

Protein Families

Steroid_dh

Protein Analysis

360

Amino Acids

40.64

Weight (kDa)

9.07

Isoelectric Point (pI)

48.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Steroid_dh	PF02544	245 - 359	1.4e-26	3-oxo-5-alpha-steroid 4-dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002229)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G72590 AT2G16530 AT2G16530 AT2G16530 AT2G16530 AT2G16530 AT3G43840 AT3G43840
fragaria_vesca	FvH4_2g10631 FvH4_2g10631 FvH4_2g10631 FvH4_2g10640 FvH4_2g10640 FvH4_2g10640
malus_domestica	MD05G1054700.v1.1 MD10G1062200.v1.1
prunus_persica	Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1
pyrus_communis	pycom05g04490 pycom05g04510 pycom10g04790
rosa_chinensis	RchiOBHm_Chr6g0268311 RchiOBHm_Chr6g0268321 RchiOBHm_Chr6g0268341
rosa_multiflora	Rmu_sc0009812.1_g000007 Rmu_sc0010202.1_g000011
rosa_roxburghii	Rroxscaffold_7G00199860 Rroxscaffold_7G00199880
rosa_rugosa	Rorug06G0040400 Rorug06G0040400 Rorug06G0040400
rosa_samantha	Rh6AG160500 Rh6BG164800 Rh6BG164900 Rh6CG153500 Rh6DG150600
rosa_wichuraiana	Rw0G000500 Rw6G013880 Rw6G013890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1017
AccB1I	GGYRCC	3 cut(s)	322, 489, 1017
AccI	GTMKAC	1 cut(s)	1074
AccII	CGCG	2 cut(s)	533, 535
AciI	CCGC	5 cut(s)	107, 132, 531, 535, 1001
AclWI	GGATC	2 cut(s)	769, 782
AcsI	RAATTY	3 cut(s)	626, 689, 1028
AcyI	GRCGYC	1 cut(s)	490
AfaI	GTAC	2 cut(s)	138, 1019
AfiI	CCNNNNNNNGG	5 cut(s)	49, 146, 192, 534, 1050
AgsI	TTSAA	7 cut(s)	357, 479, 517, 633, 724, 869, 1034
AluBI	AGCT	7 cut(s)	36, 170, 525, 647, 727, 755, 1038
AluI	AGCT	7 cut(s)	36, 170, 525, 647, 727, 755, 1038
Alw26I	GTCTC	1 cut(s)	494
AlwI	GGATC	2 cut(s)	769, 782
AoxI	GGCC	3 cut(s)	153, 562, 916
ApeKI	GCWGC	5 cut(s)	581, 600, 752, 995, 998
ApoI	RAATTY	3 cut(s)	626, 689, 1028
Asp718I	GGTACC	1 cut(s)	1017
AspLEI	GCGC	3 cut(s)	492, 535, 786
AsuHPI	GGTGA	1 cut(s)	869
BamHI	GGATCC	1 cut(s)	774
BanI	GGYRCC	3 cut(s)	322, 489, 1017
BbvI	GCAGC	5 cut(s)	587, 593, 764, 1007, 1010
BccI	CCATC	4 cut(s)	158, 463, 786, 895
BceAI	ACGGC	2 cut(s)	119, 1067
BcoDI	GTCTC	1 cut(s)	494
BfaI	CTAG	1 cut(s)	930
BfmI	CTRYAG	2 cut(s)	267, 520
BfoI	RGCGCY	2 cut(s)	493, 787
BglII	AGATCT	1 cut(s)	943
BisI	GCNGC	7 cut(s)	108, 132, 582, 601, 753, 996, 999
BlsI	GCNGC	7 cut(s)	109, 133, 583, 602, 754, 997, 1000
BmiI	GGNNCC	6 cut(s)	324, 417, 491, 737, 776, 1019
BmrI	ACTGGG	1 cut(s)	384
BmsI	GCATC	1 cut(s)	528
BmuI	ACTGGG	1 cut(s)	384
BpmI	CTGGAG	3 cut(s)	635, 722, 1007
BpuEI	CTTGAG	1 cut(s)	505
BsaHI	GRCGYC	1 cut(s)	490
BsaXI	ACNNNNNCTCC	2 cut(s)	509, 539
Bsc4I	CCNNNNNNNGG	5 cut(s)	49, 146, 192, 534, 1050
Bse1I	ACTGG	3 cut(s)	379, 565, 739
BseGI	GGATG	1 cut(s)	543
BseLI	CCNNNNNNNGG	5 cut(s)	49, 146, 192, 534, 1050
BseMII	CTCAG	3 cut(s)	230, 639, 888
BseNI	ACTGG	3 cut(s)	379, 565, 739
BseRI	GAGGAG	1 cut(s)	517
BseXI	GCAGC	5 cut(s)	587, 593, 764, 1007, 1010
BsgI	GTGCAG	1 cut(s)	771
Bsh1236I	CGCG	2 cut(s)	533, 535
BshFI	GGCC	3 cut(s)	155, 564, 918
BshNI	GGYRCC	3 cut(s)	322, 489, 1017
BslI	CCNNNNNNNGG	5 cut(s)	49, 146, 192, 534, 1050
BsmAI	GTCTC	1 cut(s)	494
BsnI	GGCC	3 cut(s)	155, 564, 918
Bsp143I	GATC	4 cut(s)	198, 774, 817, 943
BspACI	CCGC	5 cut(s)	107, 132, 531, 535, 1001
BspANI	GGCC	3 cut(s)	155, 564, 918
BspCNI	CTCAG	3 cut(s)	229, 640, 889
BspFNI	CGCG	2 cut(s)	533, 535
BspLI	GGNNCC	6 cut(s)	324, 417, 491, 737, 776, 1019
BspPI	GGATC	2 cut(s)	769, 782
BspT107I	GGYRCC	3 cut(s)	322, 489, 1017
BsrI	ACTGG	3 cut(s)	379, 565, 739
BssMI	GATC	4 cut(s)	198, 774, 817, 943
BssNAI	GTATAC	1 cut(s)	1075
BssNI	GRCGYC	1 cut(s)	490
Bst1107I	GTATAC	1 cut(s)	1075
Bst4CI	ACNGT	5 cut(s)	9, 229, 322, 591, 824
Bst6I	CTCTTC	1 cut(s)	572
BstACI	GRCGYC	1 cut(s)	490
BstAPI	GCANNNNNTGC	1 cut(s)	104
BstC8I	GCNNGC	5 cut(s)	153, 364, 677, 916, 1056
BstDEI	CTNAG	5 cut(s)	89, 216, 648, 897, 985
BstF5I	GGATG	1 cut(s)	543
BstFNI	CGCG	2 cut(s)	533, 535
BstH2I	RGCGCY	2 cut(s)	493, 787
BstHHI	GCGC	3 cut(s)	492, 535, 786
BstKTI	GATC	4 cut(s)	201, 777, 820, 946
BstMAI	GTCTC	1 cut(s)	494
BstMBI	GATC	4 cut(s)	198, 774, 817, 943
BstMWI	GCNNNNNNNGC	3 cut(s)	104, 672, 733
BstNSI	RCATGY	1 cut(s)	679
BstSFI	CTRYAG	2 cut(s)	267, 520
BstUI	CGCG	2 cut(s)	533, 535
BstV1I	GCAGC	5 cut(s)	587, 593, 764, 1007, 1010
BstX2I	RGATCY	2 cut(s)	774, 943
BstXI	CCANNNNNNTGG	2 cut(s)	374, 746
BstYI	RGATCY	2 cut(s)	774, 943
BstZ17I	GTATAC	1 cut(s)	1075
BsuRI	GGCC	3 cut(s)	155, 564, 918
BtsCI	GGATG	1 cut(s)	543
BtsIMutI	CAGTG	4 cut(s)	372, 587, 746, 829
Cac8I	GCNNGC	5 cut(s)	153, 364, 677, 916, 1056
CfoI	GCGC	3 cut(s)	492, 535, 786
Csp6I	GTAC	2 cut(s)	137, 1018
CviAII	CATG	5 cut(s)	99, 396, 676, 794, 854
CviQI	GTAC	2 cut(s)	137, 1018
DdeI	CTNAG	5 cut(s)	89, 216, 648, 897, 985
DinI	GGCGCC	1 cut(s)	491
DpnI	GATC	4 cut(s)	200, 776, 819, 945
DpnII	GATC	4 cut(s)	198, 774, 817, 943
Eam1104I	CTCTTC	1 cut(s)	572
EarI	CTCTTC	1 cut(s)	572
Eco32I	GATATC	1 cut(s)	554
EcoRV	GATATC	1 cut(s)	554
EgeI	GGCGCC	1 cut(s)	491
EheI	GGCGCC	1 cut(s)	491
FaeI	CATG	5 cut(s)	102, 399, 679, 797, 857
FatI	CATG	5 cut(s)	98, 395, 675, 793, 853
FblI	GTMKAC	1 cut(s)	1074
Fnu4HI	GCNGC	7 cut(s)	108, 132, 582, 601, 753, 996, 999
FokI	GGATG	1 cut(s)	550
Fsp4HI	GCNGC	7 cut(s)	108, 132, 582, 601, 753, 996, 999
FspBI	CTAG	1 cut(s)	930
GlaI	GCGC	3 cut(s)	491, 534, 785
GluI	GCNGC	7 cut(s)	108, 132, 582, 601, 753, 996, 999
GsuI	CTGGAG	3 cut(s)	635, 722, 1007
HaeII	RGCGCY	2 cut(s)	493, 787
HaeIII	GGCC	3 cut(s)	155, 564, 918
HhaI	GCGC	3 cut(s)	492, 535, 786
Hin1I	GRCGYC	1 cut(s)	490
Hin1II	CATG	5 cut(s)	102, 399, 679, 797, 857
Hin6I	GCGC	3 cut(s)	490, 533, 784
HinP1I	GCGC	3 cut(s)	490, 533, 784
HindIII	AAGCTT	1 cut(s)	725
HinfI	GANTC	2 cut(s)	338, 847
HphI	GGTGA	1 cut(s)	869
Hpy166II	GTNNAC	6 cut(s)	225, 276, 383, 712, 827, 1075
Hpy188I	TCNGA	4 cut(s)	219, 337, 551, 558
Hpy188III	TCNNGA	4 cut(s)	43, 484, 614, 1024
Hpy8I	GTNNAC	6 cut(s)	225, 276, 383, 712, 827, 1075
HpyAV	CCTTC	1 cut(s)	363
HpyCH4III	ACNGT	5 cut(s)	9, 229, 322, 591, 824
HpyCH4V	TGCA	5 cut(s)	296, 541, 603, 752, 797
HpyF10VI	GCNNNNNNNGC	3 cut(s)	104, 672, 733
HpyF3I	CTNAG	5 cut(s)	89, 216, 648, 897, 985
Hsp92I	GRCGYC	1 cut(s)	490
Hsp92II	CATG	5 cut(s)	102, 399, 679, 797, 857
HspAI	GCGC	3 cut(s)	490, 533, 784
KasI	GGCGCC	1 cut(s)	489
KpnI	GGTACC	1 cut(s)	1021
Kzo9I	GATC	4 cut(s)	198, 774, 817, 943
LmnI	GCTCC	3 cut(s)	167, 530, 741
Lsp1109I	GCAGC	5 cut(s)	587, 593, 764, 1007, 1010
LweI	GCATC	1 cut(s)	528
MaeI	CTAG	1 cut(s)	930
MaeIII	GTNAC	4 cut(s)	3, 591, 699, 957
MalI	GATC	4 cut(s)	200, 776, 819, 945
MboI	GATC	4 cut(s)	198, 774, 817, 943
MboII	GAAGA	4 cut(s)	208, 235, 559, 562
MflI	RGATCY	2 cut(s)	774, 943
MluCI	AATT	6 cut(s)	626, 689, 704, 798, 870, 1028
Mly113I	GGCGCC	1 cut(s)	490
MmeI	TCCRAC	2 cut(s)	752, 1005
MseI	TTAA	1 cut(s)	32
MslI	CAYNNNNRTG	1 cut(s)	255
MspA1I	CMGCKG	1 cut(s)	1001
MvnI	CGCG	2 cut(s)	533, 535
MwoI	GCNNNNNNNGC	3 cut(s)	104, 672, 733
NarI	GGCGCC	1 cut(s)	490
NdeII	GATC	4 cut(s)	198, 774, 817, 943
NlaIII	CATG	5 cut(s)	102, 399, 679, 797, 857
NlaIV	GGNNCC	6 cut(s)	324, 417, 491, 737, 776, 1019
NmuCI	GTSAC	2 cut(s)	3, 591
NspI	RCATGY	1 cut(s)	679
PaeI	GCATGC	1 cut(s)	679
PcsI	WCGNNNNNNNCGW	2 cut(s)	132, 308
PfeI	GAWTC	2 cut(s)	338, 847
PkrI	GCNGC	7 cut(s)	109, 133, 583, 602, 754, 997, 1000
PluTI	GGCGCC	1 cut(s)	493
PspN4I	GGNNCC	6 cut(s)	324, 417, 491, 737, 776, 1019
PsuI	RGATCY	2 cut(s)	774, 943
RsaI	GTAC	2 cut(s)	138, 1019
RsaNI	GTAC	2 cut(s)	137, 1018
RseI	CAYNNNNRTG	1 cut(s)	255
SaqAI	TTAA	1 cut(s)	32
SatI	GCNGC	7 cut(s)	108, 132, 582, 601, 753, 996, 999
Sau3AI	GATC	4 cut(s)	198, 774, 817, 943
SfaNI	GCATC	1 cut(s)	528
SfcI	CTRYAG	2 cut(s)	267, 520
SfoI	GGCGCC	1 cut(s)	491
SmiMI	CAYNNNNRTG	1 cut(s)	255
SmlI	CTYRAG	1 cut(s)	484
SmoI	CTYRAG	1 cut(s)	484
SphI	GCATGC	1 cut(s)	679
Sse9I	AATT	6 cut(s)	626, 689, 704, 798, 870, 1028
SsiI	CCGC	5 cut(s)	107, 132, 531, 535, 1001
SspDI	GGCGCC	1 cut(s)	489
SspMI	CTAG	1 cut(s)	930
TaaI	ACNGT	5 cut(s)	9, 229, 322, 591, 824
TaqI	TCGA	1 cut(s)	302
TaqII	GACCGA	1 cut(s)	439
TasI	AATT	6 cut(s)	626, 689, 704, 798, 870, 1028
TauI	GCSGC	2 cut(s)	110, 134
TfiI	GAWTC	2 cut(s)	338, 847
Tru1I	TTAA	1 cut(s)	32
Tru9I	TTAA	1 cut(s)	32
TscAI	CASTG	4 cut(s)	379, 594, 746, 829
TseFI	GTSAC	2 cut(s)	3, 591
TseI	GCWGC	5 cut(s)	581, 600, 752, 995, 998
Tsp45I	GTSAC	2 cut(s)	3, 591
TspDTI	ATGAA	5 cut(s)	384, 398, 643, 852, 867
TspRI	CASTG	4 cut(s)	379, 594, 746, 829
XapI	RAATTY	3 cut(s)	626, 689, 1028
XceI	RCATGY	1 cut(s)	679
XcmI	CCANNNNNNNNNTGG	1 cut(s)	988
XmiI	GTMKAC	1 cut(s)	1074
XspI	CTAG	1 cut(s)	930

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.