Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0002229

Physical Location & Seq

Genomic Coordinates Forward (+)

8399924 .. 8403346

Gene Structure 3423 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1062200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1017 bp

ATGGAGGTTGGTGTTGTTGGGTTGCTTAGAACAGCATGGATTGCTGCGACTCTGCCTATACTCATAGCCGCTCTGCCTTTTTCTTGGCTGAGCTCGTTTCGTGGGGCTGTACTGGGGTTTGCCAAGAGAGGGAAGACCATGCAGTCGTCCTCCCAGAAGTTCACAGTTCCTCAAAAGTTCTTCTGCCATTTCTATTTGGTAGCCGTAGCGTGGACAACCGTATTGCTCGTTGCAACTTTGATGTATGCGTATAAAGCGGTGCCATTGGTTTCAGATTCGTTGCTTCATCCTTCACTCCCCAACCCCTACACTGGAGGTTCACATATCTTTTCATGGCACAAGTCTCATTTGATTCCTATCAGTTATAAATACAGCGTTTGGCATTCGGTCCTTCTGCTTGTATTGATGGAGGTTCAAGTCTTGAGACGCCTCTTTGAGACAATATATGTGTTCAACTATAGCTCCTCCGCTCGGATGCACATTTTCGGATATCTGACTGGCCTCTTCTTCTACACAGCAGCACCGCTGTCACTTTGCTGCAATAATATTGCGGACATTTATAAATTTTCGATAAATGGAGTGGCTGAGTTCATTGTCAAAGGCAAGAGTAGCATGCGAGAAGTGGAATTTGATTGGTTGCAACTCGTGAATTCTCTTTTGAAGCTTGGTTGGCTCCAGTGGATTGGTGCAGCTATATTTTTTTGGGGTTGGATCCATCAGCGCCAATGTCATGCAATTCTTGGCTCGTTGCGAGAAAACAGTGAACAAAATGATGAATATGTGATTCCTCACGGTGACTGGTTTGAAATTGTTTCATCTCCACACTATCTGGCTGAGATAGTTATATATGCTGGCCTTGTGGTCGCTAGTGGAGGAACAGATCTCACAATCTGGTTACTTTTTGGATTTGTGGTGACAAACTTGGTATTGGCAGCAGCAGAAACACATAGGTGGTACCTCCGGAAATTTGAAAGTTATCCCAAGATACGACGCGCCATTATTCCATTTGTATACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003865 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0006066 GO:0006464 GO:0006486 GO:0006487 GO:0006629 GO:0006720 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0012505 GO:0016093 GO:0016229 GO:0016491 GO:0016627 GO:0019538 GO:0033765 GO:0034645 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0055114 GO:0070085 GO:0071704 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko00140 map00140

Pfam Domains

Protein Families

Steroid_dh

Protein Analysis

339

Amino Acids

38.5

Weight (kDa)

9.14

Isoelectric Point (pI)

47.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Steroid_dh	PF02544	226 - 338	2e-27	3-oxo-5-alpha-steroid 4-dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002229)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G72590 AT2G16530 AT2G16530 AT2G16530 AT2G16530 AT2G16530 AT3G43840 AT3G43840
fragaria_vesca	FvH4_2g10631 FvH4_2g10631 FvH4_2g10631 FvH4_2g10640 FvH4_2g10640 FvH4_2g10640
malus_domestica	MD05G1054700.v1.1 MD10G1062200.v1.1
prunus_persica	Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1
pyrus_communis	pycom05g04490 pycom05g04510 pycom10g04790
rosa_chinensis	RchiOBHm_Chr6g0268311 RchiOBHm_Chr6g0268321 RchiOBHm_Chr6g0268341
rosa_multiflora	Rmu_sc0009812.1_g000007 Rmu_sc0010202.1_g000011
rosa_roxburghii	Rroxscaffold_7G00199860 Rroxscaffold_7G00199880
rosa_rugosa	Rorug06G0040400 Rorug06G0040400 Rorug06G0040400
rosa_samantha	Rh6AG160500 Rh6BG164800 Rh6BG164900 Rh6CG153500 Rh6DG150600
rosa_wichuraiana	Rw0G000500 Rw6G013880 Rw6G013890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	366, 561
AasI	GACNNNNNNGTC	1 cut(s)	142
Acc65I	GGTACC	1 cut(s)	954
AccB1I	GGYRCC	2 cut(s)	259, 954
AccBSI	CCGCTC	2 cut(s)	71, 470
AccI	GTMKAC	1 cut(s)	1011
AccII	CGCG	1 cut(s)	993
AccIII	TCCGGA	1 cut(s)	960
AciI	CCGC	5 cut(s)	69, 257, 468, 524, 551
AclWI	GGATC	2 cut(s)	706, 719
AcsI	RAATTY	4 cut(s)	563, 626, 649, 965
AcyI	GRCGYC	1 cut(s)	427
AfaI	GTAC	2 cut(s)	111, 956
AfiI	CCNNNNNNNGG	4 cut(s)	129, 210, 311, 471
AgsI	TTSAA	5 cut(s)	416, 454, 661, 806, 971
AleI	CACNNNNGTG	1 cut(s)	949
AluBI	AGCT	4 cut(s)	93, 462, 664, 692
AluI	AGCT	4 cut(s)	93, 462, 664, 692
Alw21I	GWGCWC	1 cut(s)	95
Alw26I	GTCTC	3 cut(s)	348, 418, 431
AlwI	GGATC	2 cut(s)	706, 719
Aor13HI	TCCGGA	1 cut(s)	960
AoxI	GGCC	2 cut(s)	499, 853
ApeKI	GCWGC	6 cut(s)	44, 518, 537, 689, 932, 935
ApoI	RAATTY	4 cut(s)	563, 626, 649, 965
Asp718I	GGTACC	1 cut(s)	954
AspLEI	GCGC	2 cut(s)	723, 995
AspS9I	GGNCC	1 cut(s)	388
AsuHPI	GGTGA	2 cut(s)	806, 925
AvaII	GGWCC	1 cut(s)	388
BamHI	GGATCC	1 cut(s)	711
BanI	GGYRCC	2 cut(s)	259, 954
BanII	GRGCYC	1 cut(s)	95
BauI	CACGAG	1 cut(s)	644
BbsI	GAAGAC	1 cut(s)	140
Bbv12I	GWGCWC	1 cut(s)	95
BbvI	GCAGC	6 cut(s)	31, 524, 530, 701, 944, 947
BccI	CCATC	2 cut(s)	400, 723
BceAI	ACGGC	1 cut(s)	188
BcoDI	GTCTC	3 cut(s)	348, 418, 431
BfaI	CTAG	1 cut(s)	867
BfmI	CTRYAG	1 cut(s)	457
BfoI	RGCGCY	1 cut(s)	724
BglII	AGATCT	1 cut(s)	880
BisI	GCNGC	7 cut(s)	45, 69, 519, 538, 690, 933, 936
BlpI	GCTNAGC	1 cut(s)	89
BlsI	GCNGC	7 cut(s)	46, 70, 520, 539, 691, 934, 937
Bme18I	GGWCC	1 cut(s)	388
BmgT120I	GGNCC	1 cut(s)	388
BmiI	GGNNCC	4 cut(s)	261, 674, 713, 956
BmrI	ACTGGG	1 cut(s)	122
BmsI	GCATC	1 cut(s)	465
BmuI	ACTGGG	1 cut(s)	122
BpiI	GAAGAC	1 cut(s)	140
BpmI	CTGGAG	2 cut(s)	333, 659
Bpu1102I	GCTNAGC	1 cut(s)	89
BpuEI	CTTGAG	1 cut(s)	442
BsaBI	GATNNNNATC	1 cut(s)	356
BsaHI	GRCGYC	1 cut(s)	427
BsaWI	WCCGGW	1 cut(s)	960
BsaXI	ACNNNNNCTCC	2 cut(s)	446, 476
Bsc4I	CCNNNNNNNGG	4 cut(s)	129, 210, 311, 471
Bse1I	ACTGG	5 cut(s)	117, 316, 502, 676, 803
Bse8I	GATNNNNATC	1 cut(s)	356
BseAI	TCCGGA	1 cut(s)	960
BseGI	GGATG	2 cut(s)	286, 480
BseJI	GATNNNNATC	1 cut(s)	356
BseLI	CCNNNNNNNGG	4 cut(s)	129, 210, 311, 471
BseMII	CTCAG	3 cut(s)	80, 576, 825
BseNI	ACTGG	5 cut(s)	117, 316, 502, 676, 803
BseRI	GAGGAG	1 cut(s)	454
BseXI	GCAGC	6 cut(s)	31, 524, 530, 701, 944, 947
BsgI	GTGCAG	1 cut(s)	708
Bsh1236I	CGCG	1 cut(s)	993
BshFI	GGCC	2 cut(s)	501, 855
BshNI	GGYRCC	2 cut(s)	259, 954
BsiHKAI	GWGCWC	1 cut(s)	95
BsiSI	CCGG	1 cut(s)	961
BslI	CCNNNNNNNGG	4 cut(s)	129, 210, 311, 471
BsmAI	GTCTC	3 cut(s)	348, 418, 431
BsmBI	CGTCTC	1 cut(s)	418
BsmI	GAATGC	1 cut(s)	382
BsnI	GGCC	2 cut(s)	501, 855
Bsp1286I	GDGCHC	1 cut(s)	95
Bsp13I	TCCGGA	1 cut(s)	960
Bsp143I	GATC	2 cut(s)	711, 880
Bsp1720I	GCTNAGC	1 cut(s)	89
BspACI	CCGC	5 cut(s)	69, 257, 468, 524, 551
BspANI	GGCC	2 cut(s)	501, 855
BspCNI	CTCAG	3 cut(s)	81, 577, 826
BspEI	TCCGGA	1 cut(s)	960
BspFNI	CGCG	1 cut(s)	993
BspLI	GGNNCC	4 cut(s)	261, 674, 713, 956
BspPI	GGATC	2 cut(s)	706, 719
BspT107I	GGYRCC	2 cut(s)	259, 954
BsrBI	CCGCTC	2 cut(s)	71, 470
BsrI	ACTGG	5 cut(s)	117, 316, 502, 676, 803
BssMI	GATC	2 cut(s)	711, 880
BssNAI	GTATAC	1 cut(s)	1012
BssNI	GRCGYC	1 cut(s)	427
BssSI	CACGAG	1 cut(s)	644
Bst1107I	GTATAC	1 cut(s)	1012
Bst2BI	CACGAG	1 cut(s)	644
Bst4CI	ACNGT	4 cut(s)	166, 220, 761, 794
Bst6I	CTCTTC	1 cut(s)	509
BstACI	GRCGYC	1 cut(s)	427
BstAPI	GCANNNNNTGC	1 cut(s)	41
BstC8I	GCNNGC	2 cut(s)	614, 853
BstDEI	CTNAG	4 cut(s)	26, 89, 585, 834
BstF5I	GGATG	2 cut(s)	286, 480
BstFNI	CGCG	1 cut(s)	993
BstH2I	RGCGCY	1 cut(s)	724
BstHHI	GCGC	2 cut(s)	723, 995
BstKTI	GATC	2 cut(s)	714, 883
BstMAI	GTCTC	3 cut(s)	348, 418, 431
BstMBI	GATC	2 cut(s)	711, 880
BstMWI	GCNNNNNNNGC	4 cut(s)	41, 254, 609, 670
BstNSI	RCATGY	1 cut(s)	616
BstSFI	CTRYAG	1 cut(s)	457
BstUI	CGCG	1 cut(s)	993
BstV1I	GCAGC	6 cut(s)	31, 524, 530, 701, 944, 947
BstV2I	GAAGAC	1 cut(s)	140
BstX2I	RGATCY	2 cut(s)	711, 880
BstXI	CCANNNNNNTGG	1 cut(s)	683
BstYI	RGATCY	2 cut(s)	711, 880
BstZ17I	GTATAC	1 cut(s)	1012
BsuRI	GGCC	2 cut(s)	501, 855
BtsCI	GGATG	2 cut(s)	286, 480
BtsIMutI	CAGTG	3 cut(s)	309, 683, 766
Cac8I	GCNNGC	2 cut(s)	614, 853
CfoI	GCGC	2 cut(s)	723, 995
Cfr13I	GGNCC	1 cut(s)	388
CseI	GACGC	2 cut(s)	435, 999
Csp6I	GTAC	2 cut(s)	110, 955
CviAII	CATG	5 cut(s)	36, 139, 333, 613, 731
CviQI	GTAC	2 cut(s)	110, 955
DdeI	CTNAG	4 cut(s)	26, 89, 585, 834
DpnI	GATC	2 cut(s)	713, 882
DpnII	GATC	2 cut(s)	711, 880
DrdI	GACNNNNNNGTC	1 cut(s)	142
DseDI	GACNNNNNNGTC	1 cut(s)	142
Eam1104I	CTCTTC	1 cut(s)	509
EarI	CTCTTC	1 cut(s)	509
Ecl136II	GAGCTC	1 cut(s)	93
Eco24I	GRGCYC	1 cut(s)	95
Eco32I	GATATC	1 cut(s)	491
Eco47I	GGWCC	1 cut(s)	388
Eco53kI	GAGCTC	1 cut(s)	93
EcoICRI	GAGCTC	1 cut(s)	93
EcoRI	GAATTC	1 cut(s)	649
EcoRV	GATATC	1 cut(s)	491
EcoT38I	GRGCYC	1 cut(s)	95
Esp3I	CGTCTC	1 cut(s)	418
FaeI	CATG	5 cut(s)	39, 142, 336, 616, 734
FatI	CATG	5 cut(s)	35, 138, 332, 612, 730
FblI	GTMKAC	1 cut(s)	1011
Fnu4HI	GCNGC	7 cut(s)	45, 69, 519, 538, 690, 933, 936
FokI	GGATG	2 cut(s)	273, 487
FriOI	GRGCYC	1 cut(s)	95
Fsp4HI	GCNGC	7 cut(s)	45, 69, 519, 538, 690, 933, 936
FspBI	CTAG	1 cut(s)	867
GlaI	GCGC	2 cut(s)	722, 994
GluI	GCNGC	7 cut(s)	45, 69, 519, 538, 690, 933, 936
GsuI	CTGGAG	2 cut(s)	333, 659
HaeII	RGCGCY	1 cut(s)	724
HaeIII	GGCC	2 cut(s)	501, 855
HapII	CCGG	1 cut(s)	961
HgaI	GACGC	2 cut(s)	435, 999
HhaI	GCGC	2 cut(s)	723, 995
Hin1I	GRCGYC	1 cut(s)	427
Hin1II	CATG	5 cut(s)	39, 142, 336, 616, 734
Hin6I	GCGC	2 cut(s)	721, 993
HinP1I	GCGC	2 cut(s)	721, 993
HindIII	AAGCTT	1 cut(s)	662
HinfI	GANTC	4 cut(s)	49, 275, 352, 784
HpaII	CCGG	1 cut(s)	961
HphI	GGTGA	2 cut(s)	806, 925
Hpy166II	GTNNAC	5 cut(s)	162, 213, 320, 764, 1012
Hpy188I	TCNGA	4 cut(s)	274, 474, 488, 495
Hpy188III	TCNNGA	3 cut(s)	421, 646, 961
Hpy8I	GTNNAC	5 cut(s)	162, 213, 320, 764, 1012
Hpy99I	CGWCG	1 cut(s)	993
HpyAV	CCTTC	2 cut(s)	300, 401
HpyCH4III	ACNGT	4 cut(s)	166, 220, 761, 794
HpyCH4V	TGCA	7 cut(s)	142, 233, 478, 540, 640, 689, 734
HpyF10VI	GCNNNNNNNGC	4 cut(s)	41, 254, 609, 670
HpyF3I	CTNAG	4 cut(s)	26, 89, 585, 834
Hsp92I	GRCGYC	1 cut(s)	427
Hsp92II	CATG	5 cut(s)	39, 142, 336, 616, 734
HspAI	GCGC	2 cut(s)	721, 993
Kpn2I	TCCGGA	1 cut(s)	960
KpnI	GGTACC	1 cut(s)	958
Kzo9I	GATC	2 cut(s)	711, 880
LmnI	GCTCC	2 cut(s)	467, 678
Lsp1109I	GCAGC	6 cut(s)	31, 524, 530, 701, 944, 947
LweI	GCATC	1 cut(s)	465
MaeI	CTAG	1 cut(s)	867
MaeIII	GTNAC	4 cut(s)	528, 794, 894, 913
MalI	GATC	2 cut(s)	713, 882
MbiI	CCGCTC	2 cut(s)	71, 470
MboI	GATC	2 cut(s)	711, 880
MboII	GAAGA	4 cut(s)	145, 172, 496, 499
MflI	RGATCY	2 cut(s)	711, 880
MhlI	GDGCHC	1 cut(s)	95
MluCI	AATT	6 cut(s)	563, 626, 649, 735, 807, 965
MlyI	GAGTC	1 cut(s)	43
MmeI	TCCRAC	1 cut(s)	689
MroI	TCCGGA	1 cut(s)	960
MslI	CAYNNNNRTG	1 cut(s)	949
MspA1I	CMGCKG	1 cut(s)	526
MspI	CCGG	1 cut(s)	961
Mva1269I	GAATGC	1 cut(s)	382
MvnI	CGCG	1 cut(s)	993
MwoI	GCNNNNNNNGC	4 cut(s)	41, 254, 609, 670
NdeII	GATC	2 cut(s)	711, 880
NlaIII	CATG	5 cut(s)	39, 142, 336, 616, 734
NlaIV	GGNNCC	4 cut(s)	261, 674, 713, 956
NmuCI	GTSAC	3 cut(s)	528, 794, 913
NspI	RCATGY	1 cut(s)	616
OliI	CACNNNNGTG	1 cut(s)	949
PaeI	GCATGC	1 cut(s)	616
PctI	GAATGC	1 cut(s)	382
PfeI	GAWTC	3 cut(s)	275, 352, 784
PkrI	GCNGC	7 cut(s)	46, 70, 520, 539, 691, 934, 937
PleI	GAGTC	1 cut(s)	43
PpsI	GAGTC	1 cut(s)	43
PsiI	TTATAA	2 cut(s)	366, 561
Psp124BI	GAGCTC	1 cut(s)	95
PspN4I	GGNNCC	4 cut(s)	261, 674, 713, 956
PspPI	GGNCC	1 cut(s)	388
PsuI	RGATCY	2 cut(s)	711, 880
RsaI	GTAC	2 cut(s)	111, 956
RsaNI	GTAC	2 cut(s)	110, 955
RseI	CAYNNNNRTG	1 cut(s)	949
SacI	GAGCTC	1 cut(s)	95
SatI	GCNGC	7 cut(s)	45, 69, 519, 538, 690, 933, 936
Sau3AI	GATC	2 cut(s)	711, 880
Sau96I	GGNCC	1 cut(s)	388
SchI	GAGTC	1 cut(s)	43
SduI	GDGCHC	1 cut(s)	95
SetI	ASST	9 cut(s)	9, 95, 319, 414, 464, 666, 694, 953, 960
SfaNI	GCATC	1 cut(s)	465
SfcI	CTRYAG	1 cut(s)	457
SinI	GGWCC	1 cut(s)	388
SmiMI	CAYNNNNRTG	1 cut(s)	949
SmlI	CTYRAG	1 cut(s)	421
SmoI	CTYRAG	1 cut(s)	421
SphI	GCATGC	1 cut(s)	616
Sse9I	AATT	6 cut(s)	563, 626, 649, 735, 807, 965
SsiI	CCGC	5 cut(s)	69, 257, 468, 524, 551
SspI	AATATT	1 cut(s)	547
SspMI	CTAG	1 cut(s)	867
SstI	GAGCTC	1 cut(s)	95
TaaI	ACNGT	4 cut(s)	166, 220, 761, 794
TaqI	TCGA	1 cut(s)	569
TaqII	GACCGA	1 cut(s)	376
TasI	AATT	6 cut(s)	563, 626, 649, 735, 807, 965
TatI	WGTACW	1 cut(s)	109
TauI	GCSGC	1 cut(s)	71
TfiI	GAWTC	3 cut(s)	275, 352, 784
TscAI	CASTG	3 cut(s)	316, 683, 766
TseFI	GTSAC	3 cut(s)	528, 794, 913
TseI	GCWGC	6 cut(s)	44, 518, 537, 689, 932, 935
Tsp45I	GTSAC	3 cut(s)	528, 794, 913
TspDTI	ATGAA	5 cut(s)	275, 321, 580, 789, 804
TspRI	CASTG	3 cut(s)	316, 683, 766
VpaK11BI	GGWCC	1 cut(s)	388
XapI	RAATTY	4 cut(s)	563, 626, 649, 965
XceI	RCATGY	1 cut(s)	616
XmiI	GTMKAC	1 cut(s)	1011
XspI	CTAG	1 cut(s)	867

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.