Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002763

Physical Location & Seq

Genomic Coordinates Reverse (-)

7261783 .. 7266346

Gene Structure 4564 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G16730.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2547 bp

ATGAAAATACATTCTTCTGATCATTCATGGCTTTTACTAGCCGTTCTCGTGATTCTCCTCTCCTTCTCTGGAGCTCTCTCAAGCGATGACAAAGAGAAGAAGACGAAATCGGTCGATAAAAAGAAAGAAGTAACTTATGATGGAACGTCTTTGATCATCAATGGAAATAGAGAACTCCTTTACTCTGGCTCCATACATTACCCTCGTAGCACTCCTGAAATGTGGCCAAACATCATCAAGAGAGCGAAACAAGGCGGTTTGAACACGATTCAGACATACGTTTTTTGGAACGTTCATGAGCCTGAGCAGGGAAAGTTCAATTTTTCGGGTAGAGCCGACTTGGTGAAGTTCATAAAATTAATTGAGAAGAATGGTTTGTATGTAACCTTGAGGCTTGGACCTTTTATTCAAGCTGAATGGACTCATGGAGGACTTCCTTACTGGCTGAGGGAAGTTCCCGGGATCTTCTTCCGTACCGATAATGAACCTTTCAAGGAACATACAGAGAGATATGTTAAGGTGGTACTTGACATGATGAAGGAGGAGAAGCTTTTTGCTTCACAAGGAGGCCCCATTATATTAGGACAGATTGAGAATGAGTATAGTGCGGTTCAACGAGCATATAAAGAAGATGGATTAAACTACATTAAGTGGGCATCTAAACTGGTACACTCGATGGATTTGGGGATCCCATGGGTTATGTGTAAGCAAAATGATGCTCCTGATCCTATGATCAACGCTTGTAACGGAAGACATTGCGGTGATACATTCCCTGGTCCAAACAAAGACAACAAGCCATCTTTATGGACAGAGAACTGGACTACTCAGTTCCGCGTTTTTGGCGATCCCCCAGCACAAAGATCTGTAGAAGATATAGCATATTCTGTTGCCCGGTTTTTCTCCAAGAATGGAACCCATGTTAACTACTATATGTATCACGGAGGTACTAACTTTGGAAGAACCTCTGCACATTATGTGACAACTCGTTACTACGACGATGCACCTCTTGATGAATTCGGACTGGAAAGGGAGCCTAAGTACGGTCATCTAAAGCATCTTCACAACGCCCTTAACTTGTGCAAGAAGGCACTTCTCTGGGGCCAGCCTCGAGTCGAGAAGCCTAGCAATGAAACAGAGATTCGATACTACGAGCAGCCTGGAACTAAAGTTTGTGCAGCATTTTTGGCTAACAATAACACAGAAGCTGCAGAAAAAATCAAATTCAGAGGCAAGGAATATCTTATCCCGCACCGCTCCATCAGTATTCTCCCTGACTGCAAAACCGTTGTTTATAACACCGGGGAGATCATCTCTCATCATACTTCAAGAAACTTCATGAAATCGAAAAAAGCGAATAAGAACTTTGATTTCAAAGTGTTCACCGAGTCAGTGCCAAGTAAGATCAAGGGTGACTCTTTTATACCCGTTGAACTCTACGGCTTGACCAAAGATGAGTCTGATTACGGATGGTACACAACAAGCTTTAAAATAGATGACAATGACCTCTCTAAGAAGAAAGGAGGTAAGCCTAATCTGAGGATTGCTAGTCTTGGCCACGCGTTGCATGTATGGCTCAATGGAGAATACCTTGGAAACGGACATGGTAGCCATGAAGAGAAGAGTTTCGTCTTTCAGAAGCCAGTTACTCTAAAAGAAGGAGAGAACCACTTAACCATGCTAGGTGTTTTGACGGGATTTCCGGATAGTGGATCTTATATGGAGCATAGATACACAGGTCCACGTAGTGTTTCCATCTTAGGTTTGGGCTCTGGAACACTTGATCTCACTGAAGAAAACAAATGGGGAAACAAGGTGGGTATGGAAGGCGAAAGGCTTGGTATCCACGCCGAAGAAGGATTGAAGAAGGTTAAATGGGAGAAAGCTTCAGGGAAAGAACCGGGAATGACATGGTACCAGACATACTTCGATGCACCAGAGAGCCAAAGTGCAGCAGCAATTAGGATGAACGGAATGGGGAAAGGTTTGATTTGGGTGAACGGAGAAGGTGTTGGTCGATATTGGATGTCGTTTCTATCGCCTTTAGGACAGCCTACGCAAATCGAGTATCACATCCCTAGGTCTTTCTTGAAGCCCAAGAAAAATCTTCTGGTTATATTTGAGGAAGAACCAAATGTGAAGCCAGAGCTTATCGACTTTGTCATCGTCAATAGAGATACGGTGTGTTCTTATATTGGAGAAAACTACACTCCTAGTGTCCGTCACTGGACCAGGAAGAATGACCAAGTTCAGGCCATCACTGATGACGTGCATCTCACGGCTAATCTCAAATGTTCCGGCACAAAGAAAATCTCCGCTGTGGAATTCGCAAGCTTTGGGAATCCTAATGGGACTTGTGGCAACTTCACTCTTGGAAGTTGTAATGCTCCTGTTTCTAAGAAAGTCGTTGAAAAGTACTGTTTGGGTAAAGCAGAGTGTGTGATTCCGGTGAACAAGAGTACGTTCGAGCAAGACAAAAAGGATTCATGTCCCAAAGTCGAGAAGAAGCTTGCGGTACAAGTCAAGTGTGGCCGTGACAAGAAGAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BetaGal_dom4_5 Gal_Lectin Glyco_hydro_35

Protein Analysis

848

Amino Acids

95.9

Weight (kDa)

8.83

Isoelectric Point (pI)

28.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	50 - 356	2.8e-115	Glycosyl hydrolases family 35
Glyco_hydro_42	PF02449	72 - 170	8.9e-08	Beta-galactosidase
GHD	PF17834	383 - 434	2.6e-19	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	635 - 701	1.3e-22	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	762 - 842	1.4e-17	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002763)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16730 AT4G35010
fragaria_vesca	FvH4_2g01270 FvH4_4g13900
malus_domestica	MD04G1010100.v1.1 MD05G1121000.v1.1 MD10G1095200.v1.1 MD13G1284600.v1.1
prunus_persica	Prupe.1G009200_v2.0.a1 Prupe.1G009300_v2.0.a1 Prupe.8G164800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0475241 RchiOBHm_Chr4g0415021 RchiOBHm_Chr6g0251931
rosa_laevigata	RLG00000008102 RLG00000015418 RLG00000023857
rosa_multiflora	Rmu_sc0003611.1_g000013 Rmu_sc0008157.1_g000007 Rmu_ssc0000303.1_g000015
rosa_roxburghii	Rroxscaffold_5G00358760 Rroxscaffold_6G00406440
rosa_rugosa	Rorug03G0147300 Rorug04G0131700 Rorug05G0505100 Rorug05G0505200 Rorug05G0505200
rosa_samantha	Rh3AG197300 Rh4AG191000 Rh4BG189100 Rh4CG202000 Rh4DG189500 Rh6AG016400 Rh6BG014400 Rh6CG011300 Rh6DG012600
rosa_wichuraiana	Rw3G017940 Rw4G016250 Rw6G001570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1293
Acc65I	GGTACC	1 cut(s)	1911
AccB1I	GGYRCC	1 cut(s)	1911
AccBSI	CCGCTC	1 cut(s)	1254
AccII	CGCG	2 cut(s)	834, 1559
AccIII	TCCGGA	1 cut(s)	1699
AciI	CCGC	8 cut(s)	255, 608, 759, 832, 1247, 1252, 2313, 2510
AclI	AACGTT	1 cut(s)	291
AclWI	GGATC	6 cut(s)	470, 682, 695, 719, 839, 1717
AcoI	YGGCCR	3 cut(s)	224, 1552, 2527
AcsI	RAATTY	3 cut(s)	1013, 1220, 2321
AcuI	CTGAAG	2 cut(s)	1809, 1869
AdeI	CACNNNGTG	1 cut(s)	1745
AfiI	CCNNNNNNNGG	4 cut(s)	308, 1040, 1706, 2248
AflIII	ACRYGT	1 cut(s)	1557
AjiI	CACGTC	1 cut(s)	2266
AjnI	CCWGG	3 cut(s)	772, 1156, 2228
AluBI	AGCT	9 cut(s)	74, 413, 550, 1205, 1482, 1883, 2146, 2331, 2506
AluI	AGCT	9 cut(s)	74, 413, 550, 1205, 1482, 1883, 2146, 2331, 2506
Alw21I	GWGCWC	1 cut(s)	76
AlwI	GGATC	6 cut(s)	470, 682, 695, 719, 839, 1717
AlwNI	CAGNNNCTG	1 cut(s)	1205
Ama87I	CYCGRG	2 cut(s)	458, 1107
Aor13HI	TCCGGA	1 cut(s)	1699
AoxI	GGCC	6 cut(s)	224, 568, 1099, 1552, 2250, 2527
ApeKI	GCWGC	5 cut(s)	1153, 1175, 1205, 1949, 1952
ApoI	RAATTY	3 cut(s)	1013, 1220, 2321
AseI	ATTAAT	1 cut(s)	359
Asp700I	GAANNNNTTC	1 cut(s)	1622
Asp718I	GGTACC	1 cut(s)	1911
AspA2I	CCTAGG	1 cut(s)	2075
AspS9I	GGNCC	6 cut(s)	398, 569, 776, 1099, 1736, 2226
AsuC2I	CCSGG	5 cut(s)	459, 460, 892, 1300, 1899
AsuHPI	GGTGA	6 cut(s)	355, 773, 1372, 1421, 2005, 2458
AvaI	CYCGRG	2 cut(s)	458, 1107
AvaII	GGWCC	4 cut(s)	398, 776, 1736, 2226
AvrII	CCTAGG	1 cut(s)	2075
BaeI	ACNNNNGTAYC	2 cut(s)	1720, 1753
BalI	TGGCCA	2 cut(s)	226, 1554
BamHI	GGATCC	1 cut(s)	687
BanI	GGYRCC	1 cut(s)	1911
BanII	GRGCYC	2 cut(s)	76, 1769
BauI	CACGAG	1 cut(s)	47
BbsI	GAAGAC	2 cut(s)	107, 757
Bbv12I	GWGCWC	1 cut(s)	76
BbvCI	CCTCAGC	1 cut(s)	446
BbvI	GCAGC	5 cut(s)	1165, 1187, 1192, 1961, 1964
BccI	CCATC	8 cut(s)	134, 626, 670, 805, 1265, 1461, 1760, 2261
BceAI	ACGGC	4 cut(s)	26, 1453, 2292, 2514
BciT130I	CCWGG	3 cut(s)	774, 1158, 2230
BciVI	GTATCC	1 cut(s)	1850
BclI	TGATCA	3 cut(s)	19, 153, 732
BcnI	CCSGG	5 cut(s)	459, 460, 892, 1300, 1899
BfaI	CTAG	6 cut(s)	38, 1122, 1545, 1679, 2076, 2211
BfmI	CTRYAG	2 cut(s)	864, 1206
BfuI	GTATCC	1 cut(s)	1850
BglII	AGATCT	1 cut(s)	860
BisI	GCNGC	5 cut(s)	1154, 1176, 1206, 1950, 1953
BlnI	CCTAGG	1 cut(s)	2075
BlsI	GCNGC	5 cut(s)	1155, 1177, 1207, 1951, 1954
BmcAI	AGTACT	1 cut(s)	2414
Bme1390I	CCNGG	8 cut(s)	459, 460, 774, 892, 1158, 1300, 1899, 2230
Bme18I	GGWCC	4 cut(s)	398, 776, 1736, 2226
BmeT110I	CYCGRG	2 cut(s)	458, 1107
BmgBI	CACGTC	1 cut(s)	2266
BmgT120I	GGNCC	6 cut(s)	398, 569, 776, 1099, 1736, 2226
BmiI	GGNNCC	7 cut(s)	190, 571, 689, 913, 1032, 1100, 1913
BmrFI	CCNGG	8 cut(s)	459, 460, 774, 892, 1158, 1300, 1899, 2230
BmsI	GCATC	6 cut(s)	665, 706, 988, 1063, 1918, 2278
BpiI	GAAGAC	2 cut(s)	107, 757
BplI	GAGNNNNNCTC	2 cut(s)	1295, 1327
BpmI	CTGGAG	1 cut(s)	90
Bpu10I	CCTNAGC	2 cut(s)	303, 446
BpuEI	CTTGAG	2 cut(s)	64, 409
BpuMI	CCSGG	5 cut(s)	459, 460, 892, 1300, 1899
BsaAI	YACGTR	1 cut(s)	1742
BsaJI	CCNNGG	6 cut(s)	458, 692, 772, 1299, 1588, 2075
BsaWI	WCCGGW	2 cut(s)	1699, 2443
BsaXI	ACNNNNNCTCC	6 cut(s)	173, 203, 1022, 1052, 1992, 2022
Bsc4I	CCNNNNNNNGG	4 cut(s)	308, 1040, 1706, 2248
Bse1I	ACTGG	6 cut(s)	446, 669, 821, 1026, 1640, 2228
Bse3DI	GCAATG	2 cut(s)	754, 1132
BseAI	TCCGGA	1 cut(s)	1699
BseBI	CCWGG	3 cut(s)	774, 1158, 2230
BseDI	CCNNGG	6 cut(s)	458, 692, 772, 1299, 1588, 2075
BseGI	GGATG	4 cut(s)	1472, 1968, 2028, 2070
BseLI	CCNNNNNNNGG	4 cut(s)	308, 1040, 1706, 2248
BseMI	GCAATG	2 cut(s)	754, 1132
BseMII	CTCAG	4 cut(s)	294, 437, 839, 1526
BseNI	ACTGG	6 cut(s)	446, 669, 821, 1026, 1640, 2228
BseRI	GAGGAG	2 cut(s)	47, 557
BseXI	GCAGC	5 cut(s)	1165, 1187, 1192, 1961, 1964
BseYI	CCCAGC	1 cut(s)	850
BsgI	GTGCAG	3 cut(s)	951, 1194, 1968
Bsh1236I	CGCG	2 cut(s)	834, 1559
Bsh1285I	CGRYCG	1 cut(s)	114
BshFI	GGCC	6 cut(s)	226, 570, 1101, 1554, 2252, 2529
BshNI	GGYRCC	1 cut(s)	1911
BsiEI	CGRYCG	1 cut(s)	114
BsiHKAI	GWGCWC	1 cut(s)	76
BsiHKCI	CYCGRG	2 cut(s)	458, 1107
BsiSI	CCGG	7 cut(s)	459, 892, 1299, 1700, 1898, 2295, 2444
BslFI	GGGAC	2 cut(s)	2362, 2472
BslI	CCNNNNNNNGG	4 cut(s)	308, 1040, 1706, 2248
BsmFI	GGGAC	2 cut(s)	2362, 2472
BsnI	GGCC	6 cut(s)	226, 570, 1101, 1554, 2252, 2529
BsoBI	CYCGRG	2 cut(s)	458, 1107
Bsp1286I	GDGCHC	2 cut(s)	76, 1769
Bsp13I	TCCGGA	1 cut(s)	1699
Bsp19I	CCATGG	1 cut(s)	692
BspACI	CCGC	8 cut(s)	255, 608, 759, 832, 1247, 1252, 2313, 2510
BspANI	GGCC	6 cut(s)	226, 570, 1101, 1554, 2252, 2529
BspCNI	CTCAG	4 cut(s)	295, 438, 838, 1527
BspEI	TCCGGA	1 cut(s)	1699
BspFNI	CGCG	2 cut(s)	834, 1559
BspHI	TCATGA	2 cut(s)	295, 1335
BspLI	GGNNCC	7 cut(s)	190, 571, 689, 913, 1032, 1100, 1913
BspMAI	CTGCAG	1 cut(s)	1210
BspPI	GGATC	6 cut(s)	470, 682, 695, 719, 839, 1717
BspT107I	GGYRCC	1 cut(s)	1911
BsrBI	CCGCTC	1 cut(s)	1254
BsrDI	GCAATG	2 cut(s)	754, 1132
BsrI	ACTGG	6 cut(s)	446, 669, 821, 1026, 1640, 2228
BssECI	CCNNGG	6 cut(s)	458, 692, 772, 1299, 1588, 2075
BssSI	CACGAG	1 cut(s)	47
BssT1I	CCWWGG	3 cut(s)	692, 1588, 2075
Bst2BI	CACGAG	1 cut(s)	47
Bst2UI	CCWGG	3 cut(s)	774, 1158, 2230
Bst4CI	ACNGT	4 cut(s)	1043, 1285, 2179, 2417
Bst6I	CTCTTC	2 cut(s)	1608, 1613
BstBAI	YACGTR	1 cut(s)	1742
BstC8I	GCNNGC	3 cut(s)	1103, 2329, 2508
BstDEI	CTNAG	8 cut(s)	303, 446, 825, 1035, 1509, 1535, 1756, 2394
BstDSI	CCRYGG	1 cut(s)	692
BstF5I	GGATG	4 cut(s)	1472, 1968, 2028, 2070
BstFNI	CGCG	2 cut(s)	834, 1559
BstMCI	CGRYCG	1 cut(s)	114
BstMWI	GCNNNNNNNGC	3 cut(s)	840, 1184, 1570
BstNI	CCWGG	3 cut(s)	774, 1158, 2230
BstNSI	RCATGY	1 cut(s)	1568
BstSCI	CCNGG	8 cut(s)	457, 458, 772, 890, 1156, 1298, 1897, 2228
BstSFI	CTRYAG	2 cut(s)	864, 1206
BstUI	CGCG	2 cut(s)	834, 1559
BstV1I	GCAGC	5 cut(s)	1165, 1187, 1192, 1961, 1964
BstV2I	GAAGAC	2 cut(s)	107, 757
BstX2I	RGATCY	4 cut(s)	462, 687, 860, 1709
BstXI	CCANNNNNNTGG	1 cut(s)	804
BstYI	RGATCY	4 cut(s)	462, 687, 860, 1709
BsuI	GTATCC	1 cut(s)	1850
BsuRI	GGCC	6 cut(s)	226, 570, 1101, 1554, 2252, 2529
BtgI	CCRYGG	1 cut(s)	692
BtgZI	GCGATG	1 cut(s)	99
BtrI	CACGTC	1 cut(s)	2266
BtsCI	GGATG	4 cut(s)	1472, 1968, 2028, 2070
BtsIMutI	CAGTG	4 cut(s)	1395, 1785, 2221, 2256
Cac8I	GCNNGC	3 cut(s)	1103, 2329, 2508
CaiI	CAGNNNCTG	1 cut(s)	1205
CciI	TCATGA	2 cut(s)	295, 1335
Cfr13I	GGNCC	6 cut(s)	398, 569, 776, 1099, 1736, 2226
Cfr9I	CCCGGG	1 cut(s)	458
DdeI	CTNAG	8 cut(s)	303, 446, 825, 1035, 1509, 1535, 1756, 2394
DraI	TTTAAA	1 cut(s)	1486
DraIII	CACNNNGTG	1 cut(s)	1745
EaeI	YGGCCR	3 cut(s)	224, 1552, 2527
Eam1104I	CTCTTC	2 cut(s)	1608, 1613
EarI	CTCTTC	2 cut(s)	1608, 1613
Ecl136II	GAGCTC	1 cut(s)	74
Eco130I	CCWWGG	3 cut(s)	692, 1588, 2075
Eco24I	GRGCYC	2 cut(s)	76, 1769
Eco47I	GGWCC	4 cut(s)	398, 776, 1736, 2226
Eco53kI	GAGCTC	1 cut(s)	74
Eco57I	CTGAAG	2 cut(s)	1809, 1869
Eco88I	CYCGRG	2 cut(s)	458, 1107
EcoICRI	GAGCTC	1 cut(s)	74
EcoO109I	RGGNCCY	1 cut(s)	569
EcoRI	GAATTC	2 cut(s)	1013, 2321
EcoRII	CCWGG	3 cut(s)	772, 1156, 2228
EcoT14I	CCWWGG	3 cut(s)	692, 1588, 2075
EcoT38I	GRGCYC	2 cut(s)	76, 1769
ErhI	CCWWGG	3 cut(s)	692, 1588, 2075
FaqI	GGGAC	2 cut(s)	2362, 2472
FauI	CCCGC	1 cut(s)	1254
FbaI	TGATCA	3 cut(s)	19, 153, 732
Fnu4HI	GCNGC	5 cut(s)	1154, 1176, 1206, 1950, 1953
FokI	GGATG	4 cut(s)	1479, 1975, 2035, 2057
FriOI	GRGCYC	2 cut(s)	76, 1769
Fsp4HI	GCNGC	5 cut(s)	1154, 1176, 1206, 1950, 1953
FspBI	CTAG	6 cut(s)	38, 1122, 1545, 1679, 2076, 2211
GluI	GCNGC	5 cut(s)	1154, 1176, 1206, 1950, 1953
GsaI	CCCAGC	1 cut(s)	854
GsuI	CTGGAG	1 cut(s)	90
HaeIII	GGCC	6 cut(s)	226, 570, 1101, 1554, 2252, 2529
HapII	CCGG	7 cut(s)	459, 892, 1299, 1700, 1898, 2295, 2444
HincII	GTYRAC	1 cut(s)	922
HindII	GTYRAC	1 cut(s)	922
HindIII	AAGCTT	5 cut(s)	548, 1480, 1881, 2329, 2504
HpaI	GTTAAC	1 cut(s)	922
HpaII	CCGG	7 cut(s)	459, 892, 1299, 1700, 1898, 2295, 2444
HphI	GGTGA	6 cut(s)	355, 773, 1372, 1421, 2005, 2458
Hpy166II	GTNNAC	7 cut(s)	670, 922, 1380, 1473, 1739, 1996, 2449
Hpy188I	TCNGA	7 cut(s)	19, 273, 1019, 1226, 1459, 1536, 1635
Hpy8I	GTNNAC	7 cut(s)	670, 922, 1380, 1473, 1739, 1996, 2449
Hpy99I	CGWCG	1 cut(s)	998
HpyAV	CCTTC	8 cut(s)	73, 532, 1078, 1649, 1817, 1847, 1858, 1997
HpyCH4III	ACNGT	4 cut(s)	1043, 1285, 2179, 2417
HpyCH4IV	ACGT	6 cut(s)	146, 279, 291, 1741, 2265, 2459
HpyF10VI	GCNNNNNNNGC	3 cut(s)	840, 1184, 1570
HpyF3I	CTNAG	8 cut(s)	303, 446, 825, 1035, 1509, 1535, 1756, 2394
HpySE526I	ACGT	6 cut(s)	146, 279, 291, 1741, 2265, 2459
Kpn2I	TCCGGA	1 cut(s)	1699
KpnI	GGTACC	1 cut(s)	1915
Ksp22I	TGATCA	3 cut(s)	19, 153, 732
KspAI	GTTAAC	1 cut(s)	922
LmnI	GCTCC	7 cut(s)	71, 194, 724, 1030, 1259, 1720, 2389
Lsp1109I	GCAGC	5 cut(s)	1165, 1187, 1192, 1961, 1964
LweI	GCATC	6 cut(s)	665, 706, 988, 1063, 1918, 2278
MaeI	CTAG	6 cut(s)	38, 1122, 1545, 1679, 2076, 2211
MaeII	ACGT	6 cut(s)	146, 279, 291, 1741, 2265, 2459
MaeIII	GTNAC	9 cut(s)	130, 382, 743, 976, 986, 1409, 1642, 2219, 2531
MbiI	CCGCTC	1 cut(s)	1254
MflI	RGATCY	4 cut(s)	462, 687, 860, 1709
MhlI	GDGCHC	2 cut(s)	76, 1769
MlsI	TGGCCA	2 cut(s)	226, 1554
MluCI	AATT	7 cut(s)	319, 356, 360, 1013, 1220, 1956, 2321
MluI	ACGCGT	1 cut(s)	1557
MluNI	TGGCCA	2 cut(s)	226, 1554
MlyI	GAGTC	5 cut(s)	415, 1119, 1394, 1406, 1463
Mox20I	TGGCCA	2 cut(s)	226, 1554
MroI	TCCGGA	1 cut(s)	1699
MroXI	GAANNNNTTC	1 cut(s)	1622
MscI	TGGCCA	2 cut(s)	226, 1554
MseI	TTAA	9 cut(s)	359, 516, 638, 648, 921, 1071, 1485, 1670, 1869
MslI	CAYNNNNRTG	2 cut(s)	759, 802
Msp20I	TGGCCA	2 cut(s)	226, 1554
MspA1I	CMGCKG	1 cut(s)	2315
MspI	CCGG	7 cut(s)	459, 892, 1299, 1700, 1898, 2295, 2444
MspR9I	CCNGG	8 cut(s)	459, 460, 774, 892, 1158, 1300, 1899, 2230
MvaI	CCWGG	3 cut(s)	774, 1158, 2230
MvnI	CGCG	2 cut(s)	834, 1559
MwoI	GCNNNNNNNGC	3 cut(s)	840, 1184, 1570
NciI	CCSGG	5 cut(s)	459, 460, 892, 1300, 1899
NcoI	CCATGG	1 cut(s)	692
NlaIV	GGNNCC	7 cut(s)	190, 571, 689, 913, 1032, 1100, 1913
NmuCI	GTSAC	4 cut(s)	976, 1409, 2219, 2531
NspI	RCATGY	1 cut(s)	1568
PaeR7I	CTCGAG	1 cut(s)	1107
PagI	TCATGA	2 cut(s)	295, 1335
PcsI	WCGNNNNNNNCGW	1 cut(s)	1349
PdmI	GAANNNNTTC	1 cut(s)	1622
PfeI	GAWTC	6 cut(s)	52, 268, 1138, 2338, 2440, 2480
PflFI	GACNNNGTC	1 cut(s)	2156
PkrI	GCNGC	5 cut(s)	1155, 1177, 1207, 1951, 1954
PleI	GAGTC	5 cut(s)	415, 1118, 1393, 1406, 1462
PpsI	GAGTC	5 cut(s)	415, 1118, 1393, 1406, 1462
Ppu21I	YACGTR	1 cut(s)	1742
PshBI	ATTAAT	1 cut(s)	359
PsiI	TTATAA	1 cut(s)	1293
Psp124BI	GAGCTC	1 cut(s)	76
Psp1406I	AACGTT	1 cut(s)	291
Psp6I	CCWGG	3 cut(s)	772, 1156, 2228
PspFI	CCCAGC	1 cut(s)	850
PspGI	CCWGG	3 cut(s)	772, 1156, 2228
PspN4I	GGNNCC	7 cut(s)	190, 571, 689, 913, 1032, 1100, 1913
PspPI	GGNCC	6 cut(s)	398, 569, 776, 1099, 1736, 2226
PspXI	VCTCGAGB	1 cut(s)	1107
PstI	CTGCAG	1 cut(s)	1210
PstNI	CAGNNNCTG	1 cut(s)	1205
PsuI	RGATCY	4 cut(s)	462, 687, 860, 1709
PsyI	GACNNNGTC	1 cut(s)	2156
RseI	CAYNNNNRTG	2 cut(s)	759, 802
SacI	GAGCTC	1 cut(s)	76
SaqAI	TTAA	9 cut(s)	359, 516, 638, 648, 921, 1071, 1485, 1670, 1869
SatI	GCNGC	5 cut(s)	1154, 1176, 1206, 1950, 1953
Sau96I	GGNCC	6 cut(s)	398, 569, 776, 1099, 1736, 2226
ScaI	AGTACT	1 cut(s)	2414
SchI	GAGTC	5 cut(s)	415, 1119, 1394, 1406, 1463
ScrFI	CCNGG	8 cut(s)	459, 460, 774, 892, 1158, 1300, 1899, 2230
SduI	GDGCHC	2 cut(s)	76, 1769
SfaNI	GCATC	6 cut(s)	665, 706, 988, 1063, 1918, 2278
SfcI	CTRYAG	2 cut(s)	864, 1206
Sfr274I	CTCGAG	1 cut(s)	1107
SinI	GGWCC	4 cut(s)	398, 776, 1736, 2226
SlaI	CTCGAG	1 cut(s)	1107
SmaI	CCCGGG	1 cut(s)	460
SmiMI	CAYNNNNRTG	2 cut(s)	759, 802
SmlI	CTYRAG	3 cut(s)	79, 388, 1107
SmoI	CTYRAG	3 cut(s)	79, 388, 1107
Sse9I	AATT	7 cut(s)	319, 356, 360, 1013, 1220, 1956, 2321
SsiI	CCGC	8 cut(s)	255, 608, 759, 832, 1247, 1252, 2313, 2510
SspMI	CTAG	6 cut(s)	38, 1122, 1545, 1679, 2076, 2211
SstI	GAGCTC	1 cut(s)	76
StyD4I	CCNGG	8 cut(s)	457, 458, 772, 890, 1156, 1298, 1897, 2228
StyI	CCWWGG	3 cut(s)	692, 1588, 2075
TaaI	ACNGT	4 cut(s)	1043, 1285, 2179, 2417
TaiI	ACGT	6 cut(s)	149, 282, 294, 1744, 2268, 2462
TaqII	GACCGA	1 cut(s)	100
TasI	AATT	7 cut(s)	319, 356, 360, 1013, 1220, 1956, 2321
TatI	WGTACW	1 cut(s)	2412
TfiI	GAWTC	6 cut(s)	52, 268, 1138, 2338, 2440, 2480
Tru1I	TTAA	9 cut(s)	359, 516, 638, 648, 921, 1071, 1485, 1670, 1869
Tru9I	TTAA	9 cut(s)	359, 516, 638, 648, 921, 1071, 1485, 1670, 1869
TscAI	CASTG	4 cut(s)	1395, 1792, 2228, 2263
TseFI	GTSAC	4 cut(s)	976, 1409, 2219, 2531
TseI	GCWGC	5 cut(s)	1153, 1175, 1205, 1949, 1952
Tsp45I	GTSAC	4 cut(s)	976, 1409, 2219, 2531
TspGWI	ACGGA	8 cut(s)	461, 762, 954, 1479, 1611, 1983, 2013, 2207
TspMI	CCCGGG	1 cut(s)	458
TspRI	CASTG	4 cut(s)	1395, 1792, 2228, 2263
Tth111I	GACNNNGTC	1 cut(s)	2156
VpaK11BI	GGWCC	4 cut(s)	398, 776, 1736, 2226
VspI	ATTAAT	1 cut(s)	359
XapI	RAATTY	3 cut(s)	1013, 1220, 2321
XceI	RCATGY	1 cut(s)	1568
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1759
XhoI	CTCGAG	1 cut(s)	1107
XmaI	CCCGGG	1 cut(s)	458
XmaJI	CCTAGG	1 cut(s)	2075
XmnI	GAANNNNTTC	1 cut(s)	1622
XspI	CTAG	6 cut(s)	38, 1122, 1545, 1679, 2076, 2211
ZrmI	AGTACT	1 cut(s)	2414

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.