Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0002763

Physical Location & Seq

Genomic Coordinates Reverse (-)

29218123 .. 29220645

Gene Structure 2523 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000023857

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2523 bp

ATGACGATACGAAGCTGTAGAGTCCTCTTGATTGCTCTTGTTTCCATGGTGATCTCAGCCGGGGCGATGATAGCCCATTCCAAAGGCCATGAAGGGAATCAAACGCTTCAACATGGGCCTGTGACATACGATGGCAGGTCACTGATTATCAACGGAAAGCGAGAGCTTCTGTTTTCGGGTTCAATCCACTACACCCGAAGCACCCCAAAAATGTGGCCTGATCTTCTCCGAAAGGCCAAACAGGGCGGCTTGAATGTGATTCAGACCTATGTGTTCTGGAACATTCATGAGCCTGTGCAAGGTCAGTGCAATTTCAAAGGGAACTATGACTTGGTGAAGTTCATAAAACTAGTTCAAAAGCATGGAATGTATGTGACACTTAGAGTAGGGCCATTCATCCAAGCCGAATGGAACCACGGAGGACTCCCATATTGGCTTAGAGAAGTCCCGAATCTCCTATTCCGTACCGACAACGCACCGTACAAGTACTACATGAAGAAGTATGTGACCATGATTGTGAAAAGGATGAGGGAAGCGAAGCTATTTAGTCCACAGGGAGGTCCCATTATATTAGCACAAATTGAAAACGAGTACAATCATATCCAACTGGCGTATAAAGAGATGGGATACAGTTACGTCCGATGGGCAGCGAATATGGCGGTGAAGCAGGACACCGGAGTACCATGGATCATGTGCAAGCAGATGGACGCTCCGGATCCGGTGATCAATACTTGCAACGGAAGGCATTGTGGTGATACTTTCCAAGGGCCTAATAAGCCTAACAAGCCTGCTCTATGGACCGAAAACTGGACTGCTCAATACCGAGTATTTGGTGACCCTCCTTCCCAGAGGCCAGTGGAAGACATTGCATTTTCGGTTGCTCGTTTTGTTTCGAAAAATGGGGTTCTAACAAACTATTACATGTACCATGGAGGGACTAATTTTGGCAGAACAAGCGCCATTTTCACGACTACTCGCTACTACGACGAGGCTCCCGTTGACGAATATGGTCTGCCTAGAGACCCTAAATGGAGTCACCTTAGGGACTTACACAAGGCTTTGAAACTATGTAAGAAGCCTTTGCTTTGGGGAGTTTCTGGAGTCCAGAAGCTAGGCAAGGACACTGAGGCTCGTTTCTATGAGTTGCAGGACAACTCCACAATCTGTGCTGCCTTCTTGGCCAACAATAACTCTAGAACTGAAGCAATTGTTAATTGGCGAGGTCAGGAATACTACTTGCCGCCTCATTCCATTAGCATTCTCCCCGATTGCAAGACTGTGGTCTTCAATACTCAAACAATTGTGTCGCAACACAACGCTAGGAACTTTGTTAGATCAAAGGTTGCCAATAATTTGAAATGGAAGAAGTCTCAGGAACCAATTCCAAGCATCGTTCAAGTTCCTATCAATAGCAAGACACCTCTAGAGCTTTACAACTTGCTAAAAGATTCTTCAGACTACGCATGGTACACCACCACCCTTCGGTTGAGCAGGCGTGACTTGCCCATGAAGCCAAGTATTAAACCAGTTCTACGAATTGCAAATCTCGGTCATGCAATGCTTGTATTTGTTAATGGCAAATATGTTGGTTCGGGACATGGGAGCCACGACGAGAAGAGCTTCATCTTCCAGAAAACGGTTAGCTTTAAGGAGGGCGTCAACGAAATAGGACTATTTGCCATGACAGTGGGGCTCCCAGACAGCGGAGCCTACATGGAGCACAGGTACGCAGGGCCTCGAGAGATTATAGTTCTAGGGCTGAACACAGGAACCATAGATCTCTCACAAAATGGGTGGGGACATCATATTGGTCTGAACGGAGAAAAAGTAAAACTGTTCACCGAGGAGGGATCCAAACAAGTTCAATGGAGCAATACAAAAGGAGCAGCTCAGGCACTAACATGGTACAAGACATATTTTGATGCTCCAGAAGGACGTGACCCGGTTGCAATCCGAATGACAGGCATGGGAAAAGGAATGATTTGGGTAAATGGCCAAAGTATTGGTCGACACTGGATGTCCTTCCTTTCTCCACTCGGGCAACCTACTCAATCAGAATACCATATCCCGAGATCCTTCATTAAACCTAAACAAAATTTTCTTGTTGTATTGGAAGAGGAGCCGATGAAGCCTGATGACATTGAGATCCTAACAGTCAACAGGGACACAATCTGTAGCTTCATTACTGAGTATCATCCACCAAACGTAAAGTCGTGGGAAAGAAAAAATAGCCATTTTCGACCAGTGTTGGATGTTGTCAGAACTTCGACGGAGCTCAAGTGCCCGAACTACAAAAAGATTGTTGCTGTTGAGTTTGCAAACTTTGGTGATCCAACTGGGGTATGTGGAAGCTATGTCTTGGGTAACTGCAACTCCCCTATATCCAAAGAGATTGTAGAACAACATTGCTTGGGAAAATCTAGCTGCGCAGTCCCGATTGACTACAAGCTTTTCTTTAAGAAAAATGATGCGTGCCCAAATATCAAGAAGACACTTGCTGCTCAAGTAAAGTGTGCTTATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BetaGal_dom4_5 Gal_Lectin Glyco_hydro_35

Protein Analysis

841

Amino Acids

95.2

Weight (kDa)

9.34

Isoelectric Point (pI)

38.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	47 - 353	3.5e-116	Glycosyl hydrolases family 35
GHD	PF17834	381 - 433	1.5e-20	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	633 - 699	7.6e-25	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	759 - 838	1.6e-15	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002763)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16730 AT4G35010
fragaria_vesca	FvH4_2g01270 FvH4_4g13900
malus_domestica	MD04G1010100.v1.1 MD05G1121000.v1.1 MD10G1095200.v1.1 MD13G1284600.v1.1
prunus_persica	Prupe.1G009200_v2.0.a1 Prupe.1G009300_v2.0.a1 Prupe.8G164800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0475241 RchiOBHm_Chr4g0415021 RchiOBHm_Chr6g0251931
rosa_laevigata	RLG00000008102 RLG00000015418 RLG00000023857
rosa_multiflora	Rmu_sc0003611.1_g000013 Rmu_sc0008157.1_g000007 Rmu_ssc0000303.1_g000015
rosa_roxburghii	Rroxscaffold_5G00358760 Rroxscaffold_6G00406440
rosa_rugosa	Rorug03G0147300 Rorug04G0131700 Rorug05G0505100 Rorug05G0505200 Rorug05G0505200
rosa_samantha	Rh3AG197300 Rh4AG191000 Rh4BG189100 Rh4CG202000 Rh4DG189500 Rh6AG016400 Rh6BG014400 Rh6CG011300 Rh6DG012600
rosa_wichuraiana	Rw3G017940 Rw4G016250 Rw6G001570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	2428
Acc36I	ACCTGC	1 cut(s)	126
AccI	GTMKAC	1 cut(s)	2008
AccIII	TCCGGA	1 cut(s)	712
AciI	CCGC	4 cut(s)	246, 659, 1241, 1704
AclWI	GGATC	8 cut(s)	695, 710, 723, 1845, 1858, 2067, 2140, 2324
AcoI	YGGCCR	2 cut(s)	1179, 1993
AcsI	RAATTY	1 cut(s)	2095
AcuI	CTGAAG	2 cut(s)	1221, 1437
AcyI	GRCGYC	1 cut(s)	1656
AfaI	GTAC	9 cut(s)	466, 482, 488, 593, 681, 926, 1469, 1727, 1907
AfiI	CCNNNNNNNGG	6 cut(s)	299, 557, 807, 1482, 1636, 1703
AflIII	ACRYGT	1 cut(s)	921
AhlI	ACTAGT	1 cut(s)	349
AjiI	CACGTC	1 cut(s)	1937
AjuI	GAANNNNNNNTTGG	2 cut(s)	314, 346
Alw21I	GWGCWC	2 cut(s)	1722, 2277
Alw26I	GTCTC	2 cut(s)	1014, 1374
AlwI	GGATC	8 cut(s)	695, 710, 723, 1845, 1858, 2067, 2140, 2324
Ama87I	CYCGRG	3 cut(s)	1737, 2036, 2068
Aor13HI	TCCGGA	1 cut(s)	712
ApeKI	GCWGC	5 cut(s)	647, 1169, 1886, 2424, 2498
ApoI	RAATTY	1 cut(s)	2095
ArsI	GACNNNNNNTTYG	4 cut(s)	1990, 2022, 2195, 2227
Asp700I	GAANNNNTTC	2 cut(s)	1380, 1619
AspLEI	GCGC	2 cut(s)	959, 2429
AspS9I	GGNCC	6 cut(s)	116, 389, 560, 767, 798, 1733
AsuC2I	CCSGG	2 cut(s)	61, 1943
AsuHPI	GGTGA	9 cut(s)	61, 346, 673, 733, 764, 845, 1028, 1831, 2339
AsuII	TTCGAA	1 cut(s)	893
AvaI	CYCGRG	3 cut(s)	1737, 2036, 2068
AvaII	GGWCC	2 cut(s)	560, 798
AxyI	CCTNAGG	1 cut(s)	1040
BaeGI	GKGCMC	2 cut(s)	2285, 2477
BaeI	ACNNNNGTAYC	2 cut(s)	663, 696
BalI	TGGCCA	2 cut(s)	1181, 1995
BamHI	GGATCC	2 cut(s)	715, 1850
BanII	GRGCYC	2 cut(s)	1695, 2277
BbsI	GAAGAC	3 cut(s)	867, 1276, 2495
Bbv12I	GWGCWC	2 cut(s)	1722, 2277
BbvI	GCAGC	5 cut(s)	659, 1156, 1898, 2411, 2485
BccI	CCATC	4 cut(s)	125, 616, 636, 697
BciVI	GTATCC	1 cut(s)	620
BclI	TGATCA	1 cut(s)	723
BcnI	CCSGG	2 cut(s)	61, 1943
BcoDI	GTCTC	2 cut(s)	1014, 1374
BcuI	ACTAGT	1 cut(s)	349
BfaI	CTAG	8 cut(s)	350, 1017, 1112, 1194, 1320, 1424, 1754, 2421
BfmI	CTRYAG	2 cut(s)	16, 2173
BfoI	RGCGCY	1 cut(s)	960
BfuAI	ACCTGC	1 cut(s)	126
BfuI	GTATCC	1 cut(s)	620
BglI	GCCNNNNNGGC	1 cut(s)	1178
BglII	AGATCT	1 cut(s)	1777
BisI	GCNGC	7 cut(s)	247, 648, 1170, 1241, 1887, 2425, 2499
BlsI	GCNGC	7 cut(s)	248, 649, 1171, 1242, 1888, 2426, 2500
BmcAI	AGTACT	1 cut(s)	488
Bme1390I	CCNGG	2 cut(s)	61, 1943
Bme18I	GGWCC	2 cut(s)	560, 798
BmeT110I	CYCGRG	3 cut(s)	1737, 2036, 2068
BmgBI	CACGTC	1 cut(s)	1937
BmgT120I	GGNCC	6 cut(s)	116, 389, 560, 767, 798, 1733
BmrFI	CCNGG	2 cut(s)	61, 1943
BmrI	ACTGGG	1 cut(s)	2346
BmsI	GCATC	3 cut(s)	1398, 1912, 2458
BmuI	ACTGGG	1 cut(s)	2346
BoxI	GACNNNNGTC	1 cut(s)	1280
BpiI	GAAGAC	3 cut(s)	867, 1276, 2495
BpmI	CTGGAG	2 cut(s)	1119, 1911
Bpu10I	CCTNAGC	1 cut(s)	1890
Bpu14I	TTCGAA	1 cut(s)	893
BpuEI	CTTGAG	2 cut(s)	2261, 2487
BpuMI	CCSGG	2 cut(s)	61, 1943
BsaHI	GRCGYC	1 cut(s)	1656
BsaI	GGTCTC	1 cut(s)	1014
BsaJI	CCNNGG	7 cut(s)	45, 60, 415, 683, 763, 928, 1842
BsaWI	WCCGGW	3 cut(s)	674, 712, 718
BsaXI	ACNNNNNCTCC	8 cut(s)	1709, 1739, 1812, 1842, 2264, 2294, 2357, 2387
Bsc4I	CCNNNNNNNGG	6 cut(s)	299, 557, 807, 1482, 1636, 1703
Bse1I	ACTGG	7 cut(s)	612, 812, 854, 1526, 2018, 2243, 2341
Bse21I	CCTNAGG	1 cut(s)	1040
Bse3DI	GCAATG	3 cut(s)	864, 1563, 2404
BseAI	TCCGGA	1 cut(s)	712
BseDI	CCNNGG	7 cut(s)	45, 60, 415, 683, 763, 928, 1842
BseGI	GGATG	5 cut(s)	396, 531, 2022, 2194, 2257
BseLI	CCNNNNNNNGG	6 cut(s)	299, 557, 807, 1482, 1636, 1703
BseMI	GCAATG	3 cut(s)	864, 1563, 2404
BseMII	CTCAG	5 cut(s)	69, 1116, 1385, 1904, 2178
BseNI	ACTGG	7 cut(s)	612, 812, 854, 1526, 2018, 2243, 2341
BseRI	GAGGAG	2 cut(s)	1859, 2132
BseSI	GKGCMC	2 cut(s)	2285, 2477
BseXI	GCAGC	5 cut(s)	659, 1156, 1898, 2411, 2485
BsiHKAI	GWGCWC	2 cut(s)	1722, 2277
BsiHKCI	CYCGRG	3 cut(s)	1737, 2036, 2068
BsiSI	CCGG	5 cut(s)	60, 675, 713, 719, 1943
BslFI	GGGAC	8 cut(s)	431, 546, 949, 1058, 1608, 1812, 2177, 2417
BslI	CCNNNNNNNGG	6 cut(s)	299, 557, 807, 1482, 1636, 1703
BsmAI	GTCTC	2 cut(s)	1014, 1374
BsmFI	GGGAC	8 cut(s)	431, 546, 949, 1058, 1608, 1812, 2177, 2417
BsmI	GAATGC	1 cut(s)	1257
Bso31I	GGTCTC	1 cut(s)	1014
BsoBI	CYCGRG	3 cut(s)	1737, 2036, 2068
Bsp119I	TTCGAA	1 cut(s)	893
Bsp1286I	GDGCHC	5 cut(s)	1695, 1722, 2277, 2285, 2477
Bsp13I	TCCGGA	1 cut(s)	712
Bsp19I	CCATGG	3 cut(s)	45, 683, 928
BspACI	CCGC	4 cut(s)	246, 659, 1241, 1704
BspCNI	CTCAG	5 cut(s)	68, 1117, 1384, 1903, 2179
BspEI	TCCGGA	1 cut(s)	712
BspHI	TCATGA	1 cut(s)	286
BspMI	ACCTGC	1 cut(s)	126
BspPI	GGATC	8 cut(s)	695, 710, 723, 1845, 1858, 2067, 2140, 2324
BspQI	GCTCTTC	1 cut(s)	1610
BspT104I	TTCGAA	1 cut(s)	893
BspTNI	GGTCTC	1 cut(s)	1014
BsrDI	GCAATG	3 cut(s)	864, 1563, 2404
BsrI	ACTGG	7 cut(s)	612, 812, 854, 1526, 2018, 2243, 2341
BssECI	CCNNGG	7 cut(s)	45, 60, 415, 683, 763, 928, 1842
BssNI	GRCGYC	1 cut(s)	1656
BssT1I	CCWWGG	4 cut(s)	45, 683, 763, 928
Bst4CI	ACNGT	7 cut(s)	480, 632, 1279, 1639, 1687, 1836, 2155
Bst6I	CTCTTC	2 cut(s)	1610, 2109
BstACI	GRCGYC	1 cut(s)	1656
BstBI	TTCGAA	1 cut(s)	893
BstC8I	GCNNGC	4 cut(s)	698, 789, 1493, 2473
BstDEI	CTNAG	8 cut(s)	55, 380, 437, 1040, 1125, 1371, 1890, 2187
BstDSI	CCRYGG	4 cut(s)	45, 415, 683, 928
BstEII	GGTNACC	1 cut(s)	833
BstENI	CCTNNNNNAGG	1 cut(s)	297
BstF5I	GGATG	5 cut(s)	396, 531, 2022, 2194, 2257
BstH2I	RGCGCY	1 cut(s)	960
BstHHI	GCGC	2 cut(s)	959, 2429
BstMAI	GTCTC	2 cut(s)	1014, 1374
BstNSI	RCATGY	1 cut(s)	925
BstPAI	GACNNNNGTC	1 cut(s)	1280
BstPI	GGTNACC	1 cut(s)	833
BstSCI	CCNGG	2 cut(s)	59, 1941
BstSFI	CTRYAG	2 cut(s)	16, 2173
BstSLI	GKGCMC	2 cut(s)	2285, 2477
BstV1I	GCAGC	5 cut(s)	659, 1156, 1898, 2411, 2485
BstV2I	GAAGAC	3 cut(s)	867, 1276, 2495
BstX2I	RGATCY	5 cut(s)	715, 1777, 1850, 2072, 2145
BstXI	CCANNNNNNTGG	3 cut(s)	213, 1687, 2003
BstYI	RGATCY	5 cut(s)	715, 1777, 1850, 2072, 2145
Bsu36I	CCTNAGG	1 cut(s)	1040
BsuI	GTATCC	1 cut(s)	620
BtgI	CCRYGG	4 cut(s)	45, 415, 683, 928
BtgZI	GCGATG	1 cut(s)	80
BtrI	CACGTC	1 cut(s)	1937
BtsCI	GGATG	5 cut(s)	396, 531, 2022, 2194, 2257
BtsIMutI	CAGTG	7 cut(s)	140, 311, 861, 1122, 1692, 2011, 2250
BveI	ACCTGC	1 cut(s)	126
Cac8I	GCNNGC	4 cut(s)	698, 789, 1493, 2473
CciI	TCATGA	1 cut(s)	286
CfoI	GCGC	2 cut(s)	959, 2429
Cfr13I	GGNCC	6 cut(s)	116, 389, 560, 767, 798, 1733
CseI	GACGC	2 cut(s)	716, 1645
Csp6I	GTAC	9 cut(s)	465, 481, 487, 592, 680, 925, 1468, 1726, 1906
CspCI	CAANNNNNGTGG	2 cut(s)	1775, 1810
CviQI	GTAC	9 cut(s)	465, 481, 487, 592, 680, 925, 1468, 1726, 1906
DdeI	CTNAG	8 cut(s)	55, 380, 437, 1040, 1125, 1371, 1890, 2187
EaeI	YGGCCR	2 cut(s)	1179, 1993
Eam1104I	CTCTTC	2 cut(s)	1610, 2109
EarI	CTCTTC	2 cut(s)	1610, 2109
Ecl136II	GAGCTC	1 cut(s)	2275
Eco130I	CCWWGG	4 cut(s)	45, 683, 763, 928
Eco24I	GRGCYC	2 cut(s)	1695, 2277
Eco31I	GGTCTC	1 cut(s)	1014
Eco47I	GGWCC	2 cut(s)	560, 798
Eco53kI	GAGCTC	1 cut(s)	2275
Eco57I	CTGAAG	2 cut(s)	1221, 1437
Eco81I	CCTNAGG	1 cut(s)	1040
Eco88I	CYCGRG	3 cut(s)	1737, 2036, 2068
Eco91I	GGTNACC	1 cut(s)	833
EcoICRI	GAGCTC	1 cut(s)	2275
EcoNI	CCTNNNNNAGG	1 cut(s)	297
EcoO109I	RGGNCCY	3 cut(s)	560, 767, 1733
EcoO65I	GGTNACC	1 cut(s)	833
EcoT14I	CCWWGG	4 cut(s)	45, 683, 763, 928
EcoT38I	GRGCYC	2 cut(s)	1695, 2277
ErhI	CCWWGG	4 cut(s)	45, 683, 763, 928
FalI	AAGNNNNNCTT	2 cut(s)	2438, 2470
FaqI	GGGAC	8 cut(s)	431, 546, 949, 1058, 1608, 1812, 2177, 2417
FbaI	TGATCA	1 cut(s)	723
FblI	GTMKAC	1 cut(s)	2008
Fnu4HI	GCNGC	7 cut(s)	247, 648, 1170, 1241, 1887, 2425, 2499
FokI	GGATG	5 cut(s)	383, 538, 2029, 2181, 2264
FriOI	GRGCYC	2 cut(s)	1695, 2277
Fsp4HI	GCNGC	7 cut(s)	247, 648, 1170, 1241, 1887, 2425, 2499
FspBI	CTAG	8 cut(s)	350, 1017, 1112, 1194, 1320, 1424, 1754, 2421
FspI	TGCGCA	1 cut(s)	2428
GlaI	GCGC	2 cut(s)	958, 2428
GluI	GCNGC	7 cut(s)	247, 648, 1170, 1241, 1887, 2425, 2499
GsuI	CTGGAG	2 cut(s)	1119, 1911
HaeII	RGCGCY	1 cut(s)	960
HapII	CCGG	5 cut(s)	60, 675, 713, 719, 1943
HgaI	GACGC	2 cut(s)	716, 1645
HhaI	GCGC	2 cut(s)	959, 2429
Hin1I	GRCGYC	1 cut(s)	1656
Hin6I	GCGC	2 cut(s)	957, 2427
HinP1I	GCGC	2 cut(s)	957, 2427
HincII	GTYRAC	4 cut(s)	1000, 1660, 2009, 2158
HindII	GTYRAC	4 cut(s)	1000, 1660, 2009, 2158
HindIII	AAGCTT	1 cut(s)	2447
HinfI	GANTC	8 cut(s)	21, 97, 259, 423, 451, 1033, 1101, 1448
HpaII	CCGG	5 cut(s)	60, 675, 713, 719, 1943
HphI	GGTGA	9 cut(s)	61, 346, 673, 733, 764, 845, 1028, 1831, 2339
Hpy166II	GTNNAC	7 cut(s)	551, 1000, 1470, 1660, 1839, 2009, 2158
Hpy188I	TCNGA	8 cut(s)	230, 264, 641, 1456, 1815, 1955, 2056, 2261
Hpy8I	GTNNAC	7 cut(s)	551, 1000, 1470, 1660, 1839, 2009, 2158
Hpy99I	CGWCG	3 cut(s)	989, 1613, 2272
HpyAV	CCTTC	8 cut(s)	86, 735, 852, 1183, 1490, 1925, 2032, 2086
HpyCH4III	ACNGT	7 cut(s)	480, 632, 1279, 1639, 1687, 1836, 2155
HpyCH4IV	ACGT	3 cut(s)	636, 1936, 2205
HpyF3I	CTNAG	8 cut(s)	55, 380, 437, 1040, 1125, 1371, 1890, 2187
HpySE526I	ACGT	3 cut(s)	636, 1936, 2205
Hsp92I	GRCGYC	1 cut(s)	1656
HspAI	GCGC	2 cut(s)	957, 2427
Kpn2I	TCCGGA	1 cut(s)	712
Ksp22I	TGATCA	1 cut(s)	723
LguI	GCTCTTC	1 cut(s)	1610
Lsp1109I	GCAGC	5 cut(s)	659, 1156, 1898, 2411, 2485
LweI	GCATC	3 cut(s)	1398, 1912, 2458
MaeI	CTAG	8 cut(s)	350, 1017, 1112, 1194, 1320, 1424, 1754, 2421
MaeII	ACGT	3 cut(s)	636, 1936, 2205
MfeI	CAATTG	2 cut(s)	1206, 1299
MflI	RGATCY	5 cut(s)	715, 1777, 1850, 2072, 2145
MhlI	GDGCHC	5 cut(s)	1695, 1722, 2277, 2285, 2477
MlsI	TGGCCA	2 cut(s)	1181, 1995
MluNI	TGGCCA	2 cut(s)	1181, 1995
MlyI	GAGTC	4 cut(s)	30, 417, 1042, 1110
MmeI	TCCRAC	3 cut(s)	628, 2229, 2357
Mox20I	TGGCCA	2 cut(s)	1181, 1995
MroI	TCCGGA	1 cut(s)	712
MroXI	GAANNNNTTC	2 cut(s)	1380, 1619
MscI	TGGCCA	2 cut(s)	1181, 1995
MseI	TTAA	6 cut(s)	1212, 1521, 1572, 1647, 2082, 2457
MslI	CAYNNNNRTG	4 cut(s)	515, 750, 1685, 1900
Msp20I	TGGCCA	2 cut(s)	1181, 1995
MspA1I	CMGCKG	1 cut(s)	1704
MspI	CCGG	5 cut(s)	60, 675, 713, 719, 1943
MspR9I	CCNGG	2 cut(s)	61, 1943
MunI	CAATTG	2 cut(s)	1206, 1299
Mva1269I	GAATGC	1 cut(s)	1257
NciI	CCSGG	2 cut(s)	61, 1943
NcoI	CCATGG	3 cut(s)	45, 683, 928
NmuCI	GTSAC	8 cut(s)	121, 138, 373, 505, 833, 1034, 1496, 1937
NsbI	TGCGCA	1 cut(s)	2428
NspI	RCATGY	1 cut(s)	925
NspV	TTCGAA	1 cut(s)	893
PaeR7I	CTCGAG	1 cut(s)	1737
PagI	TCATGA	1 cut(s)	286
PciI	ACATGT	1 cut(s)	921
PciSI	GCTCTTC	1 cut(s)	1610
PcsI	WCGNNNNNNNCGW	1 cut(s)	993
PctI	GAATGC	1 cut(s)	1257
PdmI	GAANNNNTTC	2 cut(s)	1380, 1619
PfeI	GAWTC	4 cut(s)	97, 259, 451, 1448
PkrI	GCNGC	7 cut(s)	248, 649, 1171, 1242, 1888, 2426, 2500
PleI	GAGTC	4 cut(s)	29, 417, 1041, 1109
PpsI	GAGTC	4 cut(s)	29, 417, 1041, 1109
PpuMI	RGGWCCY	1 cut(s)	560
PscI	ACATGT	1 cut(s)	921
PshAI	GACNNNNGTC	1 cut(s)	1280
Psp124BI	GAGCTC	1 cut(s)	2277
Psp5II	RGGWCCY	1 cut(s)	560
PspEI	GGTNACC	1 cut(s)	833
PspPI	GGNCC	6 cut(s)	116, 389, 560, 767, 798, 1733
PspPPI	RGGWCCY	1 cut(s)	560
PsuI	RGATCY	5 cut(s)	715, 1777, 1850, 2072, 2145
RsaI	GTAC	9 cut(s)	466, 482, 488, 593, 681, 926, 1469, 1727, 1907
RsaNI	GTAC	9 cut(s)	465, 481, 487, 592, 680, 925, 1468, 1726, 1906
RseI	CAYNNNNRTG	4 cut(s)	515, 750, 1685, 1900
SacI	GAGCTC	1 cut(s)	2277
SalI	GTCGAC	1 cut(s)	2007
SapI	GCTCTTC	1 cut(s)	1610
SaqAI	TTAA	6 cut(s)	1212, 1521, 1572, 1647, 2082, 2457
SatI	GCNGC	7 cut(s)	247, 648, 1170, 1241, 1887, 2425, 2499
Sau96I	GGNCC	6 cut(s)	116, 389, 560, 767, 798, 1733
ScaI	AGTACT	1 cut(s)	488
SchI	GAGTC	4 cut(s)	30, 417, 1042, 1110
ScrFI	CCNGG	2 cut(s)	61, 1943
SduI	GDGCHC	5 cut(s)	1695, 1722, 2277, 2285, 2477
SfaNI	GCATC	3 cut(s)	1398, 1912, 2458
SfcI	CTRYAG	2 cut(s)	16, 2173
Sfr274I	CTCGAG	1 cut(s)	1737
SfuI	TTCGAA	1 cut(s)	893
SinI	GGWCC	2 cut(s)	560, 798
SlaI	CTCGAG	1 cut(s)	1737
SmiMI	CAYNNNNRTG	4 cut(s)	515, 750, 1685, 1900
SmlI	CTYRAG	3 cut(s)	1737, 2276, 2502
SmoI	CTYRAG	3 cut(s)	1737, 2276, 2502
SpeI	ACTAGT	1 cut(s)	349
SsiI	CCGC	4 cut(s)	246, 659, 1241, 1704
SspMI	CTAG	8 cut(s)	350, 1017, 1112, 1194, 1320, 1424, 1754, 2421
SstI	GAGCTC	1 cut(s)	2277
StyD4I	CCNGG	2 cut(s)	59, 1941
StyI	CCWWGG	4 cut(s)	45, 683, 763, 928
TaaI	ACNGT	7 cut(s)	480, 632, 1279, 1639, 1687, 1836, 2155
TaiI	ACGT	3 cut(s)	639, 1939, 2208
TaqI	TCGA	5 cut(s)	893, 1738, 2008, 2239, 2267
TaqII	GACCGA	2 cut(s)	815, 1538
TatI	WGTACW	2 cut(s)	486, 591
TauI	GCSGC	2 cut(s)	249, 1243
TfiI	GAWTC	4 cut(s)	97, 259, 451, 1448
Tru1I	TTAA	6 cut(s)	1212, 1521, 1572, 1647, 2082, 2457
Tru9I	TTAA	6 cut(s)	1212, 1521, 1572, 1647, 2082, 2457
TscAI	CASTG	7 cut(s)	147, 311, 861, 1129, 1692, 2018, 2250
TseFI	GTSAC	8 cut(s)	121, 138, 373, 505, 833, 1034, 1496, 1937
TseI	GCWGC	5 cut(s)	647, 1169, 1886, 2424, 2498
Tsp45I	GTSAC	8 cut(s)	121, 138, 373, 505, 833, 1034, 1496, 1937
TspGWI	ACGGA	6 cut(s)	168, 432, 452, 753, 1833, 2285
TspRI	CASTG	7 cut(s)	147, 311, 861, 1129, 1692, 2018, 2250
VpaK11BI	GGWCC	2 cut(s)	560, 798
XagI	CCTNNNNNAGG	1 cut(s)	297
XapI	RAATTY	1 cut(s)	2095
XbaI	TCTAGA	2 cut(s)	1193, 1423
XceI	RCATGY	1 cut(s)	925
XhoI	CTCGAG	1 cut(s)	1737
XmiI	GTMKAC	1 cut(s)	2008
XmnI	GAANNNNTTC	2 cut(s)	1380, 1619
XspI	CTAG	8 cut(s)	350, 1017, 1112, 1194, 1320, 1424, 1754, 2421
ZrmI	AGTACT	1 cut(s)	488

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.