MD10G1095200.v1.1

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002763
Physical Location & Seq
Forward (+)
14893753 .. 14898626
4874 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1095200.v1.1.491

Sequence Viewer

Length: 2580 bp
ATGGTGGTAGCAAAGAGGGTTCTGTTGATTTCTTTTGTTCTTTTGGTGGTCTCGCCCAGTCTATGTTATGCCAAAGGTAACAAGGCAGCTCCTGCTGCGGATGGGGAAACAGTGGAAGATACGGTGGAAGGGGAAGAGCCGGTTGTTGGAAAGAGCGAGAAGAAACTTGAAGTCGGGCAGGTGACATATGATGGCAGGTCCATCATGATCAATGGAAAACGAGAGATTTTCTTGTCGGGTTCAATTCATTATCCGCGGAGCACGGTGGATATGTGGCCTGATCTTCTGAAGAAGGCTAAGCGGGGAGGTTTGAACGTGATTCAAACTTACGTCTTTTGGAACCTTCATGAGCCCGTGAAAGGCAAGTGGAATTTCGAGGGAAACGCCGATGTGGTGAAGTTCATCAGGCTAGTTCAGGAGAACAATATGTATGTCACCCTCAGGGTAGGGCCTTTCATCCAAGCCGAATGGAACCACGGAGGGCTTCCATATTGGCTGAGGGAGGAACCAAACATCATTTTCCGTTCGGATAATCCAGCATACAAGCAACACATGGAGGCTTACGTGACGAAGATAATGAAGATGATGAAAGATGAGAAGCTATTTGCTCCACAGGGTGGTCCAATCATCTTAGCCCAGATTGAAAATGAGTATACTCATGTCCAGCTTGCATATAAAGAGTTGGGGACCAATTATGTGCAGTGGGCAGCAAATATGGCGCTTGCGCAGAACATCGGCGTGCCATGGATCATGTGCAAACAGACGAATGCTCCTGATCCAATCATTAATACTTGCAATGGAAGGCACTGTGGAGACACTTTTGCAGGCCCTAATAAGCCTCATAAGCCTTCTCTATGGACTGAGAACTGGACTGCTCAGTACAGAGTATTCGGTGATCCTCCTTCACAAAGATCGGCAGAAGACATTGCGTTTTCAGTTGCTCGATTTTTCTCCAAGGACGGAACCCTAACCAACTACTATATGTACCATGGTGGAACAAACTTTGGAAGAACAAGTGCTATTTTCACAACAACTCGATACTATGACGAAGCTCCTCTCGATGAATATGGTTTGCAAAGGGAGCCGAAATGGAGTCACCTAAGGGACTTGCACAAGGCTTTGAATCTATGCAAGAAGCCTTTGTTGGCAGGAGCTTCTGGAGTAGTAAGGTTGGGCAAAGAAACTGAGGCTCGTTTCTATGAGAGGCCCGGAACAGACATTTGTGTTGCTTTCTTGGCAAACAACCACTCAAAACTAGAAACAAGTGTCACTTGGCGTGGGCAGGAGTATTTCCTGCCACCACATTCCATTAGCATACTCCCGGATTGCAAGACCGTGGTCTTCAATTCTCAACAGATTGTGTCACAGCATAATTCGAGGAACTTTGTTCGATCAAAGGCTGCTAATAAACTCAACTGGGAAATGTCTCCAGAACCAATTCCGACCGTTGAGCAAGTGCCTGTGAATCAAAAGAAACCACAGGAACTTTACCACTTGCTCAAAGATGCCACAGACTATGGATGGTTCACCACTAGCCTTAACCTGGGTCCATATGACTTGCCGATGAAGGATTCTATTCGCCCAGTTATCCGAATCCCAAGTCTTGGTCACGCAATGGGCGTATTCGTCAATGGTGAATATATTGGAAGTGAACATGGAAGCCACGAGGAGAAGGGATTTGTCTTCGAAAGACCTGTCACATTCAAACAAGGGATCAATCACATATCACTGCTTTGCATGACAGTGGGACTCCCAGATAGTGGAGCATACATGGAGCACAGATACGCGGGACCCCGAACTATAAACGTCCTGGGACTGAATACCGGAACAATTGATCTCTCACAAAATGGTTGGGGGCATAGGGTTGGTTTGAATGGCGAAAATCTACAAGTGTTCACCGAAAAAGGAGCACAGAAAGTCCAATGGACTAAAGCTGTAGGCAAAGGAAGGGGTCTCACATGGTACAAGACATATTTTGATGCTCCTGAAGGGAGAGACCCTGTTGCTATCCGGATGACTGGAATGGGAAAGGGAATGATTTGGGTGAATGGTTTAAGCATCGGGCGGCATTGGATGAGCTACCTCTCTCCTCTTGGACAGCCTACTCAAGCTGAGTACCATATTCCAAGATCCCTTATCAAGCCGAGCAAAAATTTCCTTGTTGTATTGGAGGAGCAGGGTGCTGACCCCAAGGACATTGAGATCCTAACAGTCAACAGAGATACAATCTGTTCTATGAGTACACAGATGCATCCTCCACACGTTAAGGCATTCGAGAGAAAGGCAGGCAAGTTCAAAGTTGTTGCAGGCGTCCCTGGTGCAACTGCTCAGCTCAAGTGTCCAAACTACAAAAAGATTGTTGCTGTTGAGTTTGCAAGCTTTGGTAGTCCTGAAGGCGCATGTGGAACCTATGCCATTGGCAAATGTGATGCTCCAGGAACCAAGAAGATCGTTGAAGAGAAGTGCTTAGGACAGACTACATGTACAGTCCCTAGTGACCCCAAGCTATTCTTCAAAGTGGATCCTTGCCCAGATGTCAAGATCAAAACACTTGCGATCCAAGTAAAGTGTTCCCACTAA

Protein Analysis

860

Amino Acids

96.08

Weight (kDa)

8.9

Isoelectric Point (pI)

37.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 67 - 373 2.3e-115 Glycosyl hydrolases family 35
Glyco_hydro_42 PF02449 90 - 235 1e-05 Beta-galactosidase
GHD PF17834 398 - 453 1.6e-18 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 652 - 718 3.1e-23 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 778 - 857 2.5e-15 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 169
Acc16I TGCGCA 1 cut(s) 726
Acc36I ACCTGC 2 cut(s) 169, 186
AccB7I CCANNNNNTGG 3 cut(s) 617, 1604, 1760
AccI GTMKAC 1 cut(s) 653
AccII CGCG 2 cut(s) 256, 1787
AccIII TCCGGA 1 cut(s) 2010
AciI CCGC 6 cut(s) 98, 254, 256, 301, 1787, 2065
AclWI GGATC 9 cut(s) 755, 770, 890, 1721, 2124, 2197, 2516, 2529, 2551
AcsI RAATTY 2 cut(s) 370, 2152
AcuI CTGAAG 3 cut(s) 308, 2007, 2412
AcyI GRCGYC 1 cut(s) 2310
AdeI CACNNNGTG 1 cut(s) 617
AfaI GTAC 6 cut(s) 881, 986, 1964, 2117, 2242, 2485
AfiI CCNNNNNNNGG 6 cut(s) 146, 359, 617, 1543, 1604, 1760
AflIII ACRYGT 2 cut(s) 2260, 2480
AjnI CCWGG 4 cut(s) 1542, 1809, 2314, 2434
AjuI GAANNNNNNNTTGG 4 cut(s) 1127, 1159, 2495, 2527
Alw21I GWGCWC 3 cut(s) 263, 1779, 1912
Alw26I GTCTC 5 cut(s) 55, 807, 1431, 1958, 1989
AlwI GGATC 9 cut(s) 755, 770, 890, 1721, 2124, 2197, 2516, 2529, 2551
AlwNI CAGNNNCTG 1 cut(s) 92
Aor13HI TCCGGA 1 cut(s) 2010
AoxI GGCC 4 cut(s) 275, 449, 826, 1205
ApeKI GCWGC 4 cut(s) 86, 95, 707, 1400
ApoI RAATTY 2 cut(s) 370, 2152
ArsI GACNNNNNNTTYG 4 cut(s) 1585, 1617, 1680, 1712
AseI ATTAAT 1 cut(s) 786
Asp700I GAANNNNTTC 1 cut(s) 1437
AspLEI GCGC 3 cut(s) 721, 727, 2399
AspS9I GGNCC 8 cut(s) 198, 449, 620, 687, 827, 1206, 1547, 1790
AsuC2I CCSGG 2 cut(s) 1209, 1322
AsuHPI GGTGA 9 cut(s) 193, 406, 427, 905, 1088, 1519, 1646, 1888, 2056
AsuII TTCGAA 1 cut(s) 1686
AvaII GGWCC 5 cut(s) 198, 620, 687, 1547, 1790
AxyI CCTNAGG 2 cut(s) 440, 1100
BamHI GGATCC 1 cut(s) 2521
BanII GRGCYC 1 cut(s) 354
BauI CACGAG 1 cut(s) 1664
BbsI GAAGAC 3 cut(s) 927, 1333, 1675
Bbv12I GWGCWC 3 cut(s) 263, 1779, 1912
BbvCI CCTCAGC 1 cut(s) 497
BbvI GCAGC 4 cut(s) 82, 98, 719, 1387
BccI CCATC 4 cut(s) 95, 185, 209, 1515
BciT130I CCWGG 4 cut(s) 1544, 1811, 2316, 2436
BclI TGATCA 1 cut(s) 207
BcnI CCSGG 2 cut(s) 1209, 1322
BcoDI GTCTC 5 cut(s) 55, 807, 1431, 1958, 1989
BfaI CTAG 4 cut(s) 410, 1256, 1533, 2493
BfmI CTRYAG 1 cut(s) 1935
BfoI RGCGCY 1 cut(s) 722
BfuAI ACCTGC 2 cut(s) 169, 186
BisI GCNGC 5 cut(s) 87, 96, 708, 1401, 2066
BlpI GCTNAGC 2 cut(s) 297, 2328
BlsI GCNGC 5 cut(s) 88, 97, 709, 1402, 2067
Bme1390I CCNGG 6 cut(s) 1209, 1322, 1544, 1811, 2316, 2436
Bme18I GGWCC 5 cut(s) 198, 620, 687, 1547, 1790
BmgT120I GGNCC 8 cut(s) 198, 449, 620, 687, 827, 1206, 1547, 1790
BmrFI CCNGG 6 cut(s) 1209, 1322, 1544, 1811, 2316, 2436
BmrI ACTGGG 3 cut(s) 51, 1426, 1577
BmsI GCATC 6 cut(s) 1495, 1969, 2067, 2238, 2260, 2419
BmuI ACTGGG 3 cut(s) 51, 1426, 1577
BoxI GACNNNNGTC 1 cut(s) 1337
BpiI GAAGAC 3 cut(s) 927, 1333, 1675
BpmI CTGGAG 3 cut(s) 1179, 1413, 2418
Bpu10I CCTNAGC 2 cut(s) 497, 2467
Bpu1102I GCTNAGC 2 cut(s) 297, 2328
Bpu14I TTCGAA 1 cut(s) 1686
BpuEI CTTGAG 2 cut(s) 2091, 2318
BpuMI CCSGG 2 cut(s) 1209, 1322
BsaAI YACGTR 1 cut(s) 565
BsaHI GRCGYC 1 cut(s) 2310
BsaI GGTCTC 3 cut(s) 55, 1958, 1989
BsaWI WCCGGW 2 cut(s) 1823, 2010
BsaXI ACNNNNNCTCC 2 cut(s) 754, 784
Bsc4I CCNNNNNNNGG 6 cut(s) 146, 359, 617, 1543, 1604, 1760
Bse118I RCCGGY 1 cut(s) 139
Bse1I ACTGG 5 cut(s) 57, 872, 1421, 1583, 2023
Bse21I CCTNAGG 2 cut(s) 440, 1100
Bse3DI GCAATG 3 cut(s) 802, 924, 1620
BseAI TCCGGA 1 cut(s) 2010
BseBI CCWGG 4 cut(s) 1544, 1811, 2316, 2436
BseGI GGATG 6 cut(s) 106, 456, 1526, 2019, 2079, 2251
BseLI CCNNNNNNNGG 6 cut(s) 146, 359, 617, 1543, 1604, 1760
BseMI GCAATG 3 cut(s) 802, 924, 1620
BseMII CTCAG 7 cut(s) 454, 488, 852, 890, 1176, 2103, 2342
BseNI ACTGG 5 cut(s) 57, 872, 1421, 1583, 2023
BseRI GAGGAG 4 cut(s) 1044, 1682, 2079, 2186
BseXI GCAGC 4 cut(s) 82, 98, 719, 1387
BsgI GTGCAG 1 cut(s) 719
Bsh1236I CGCG 2 cut(s) 256, 1787
Bsh1285I CGRYCG 1 cut(s) 1446
BshFI GGCC 4 cut(s) 277, 451, 828, 1207
BsiEI CGRYCG 1 cut(s) 1446
BsiHKAI GWGCWC 3 cut(s) 263, 1779, 1912
BsiSI CCGG 5 cut(s) 140, 1209, 1322, 1824, 2011
BslFI GGGAC 7 cut(s) 700, 1118, 1761, 1803, 1827, 2297, 2474
BslI CCNNNNNNNGG 6 cut(s) 146, 359, 617, 1543, 1604, 1760
BsmAI GTCTC 5 cut(s) 55, 807, 1431, 1958, 1989
BsmFI GGGAC 7 cut(s) 700, 1118, 1761, 1803, 1827, 2297, 2474
BsmI GAATGC 2 cut(s) 772, 2270
BsnI GGCC 4 cut(s) 277, 451, 828, 1207
Bso31I GGTCTC 3 cut(s) 55, 1958, 1989
Bsp119I TTCGAA 1 cut(s) 1686
Bsp1286I GDGCHC 4 cut(s) 263, 354, 1779, 1912
Bsp13I TCCGGA 1 cut(s) 2010
Bsp1407I TGTACA 1 cut(s) 2483
Bsp1720I GCTNAGC 2 cut(s) 297, 2328
Bsp19I CCATGG 2 cut(s) 743, 988
BspACI CCGC 6 cut(s) 98, 254, 256, 301, 1787, 2065
BspANI GGCC 4 cut(s) 277, 451, 828, 1207
BspCNI CTCAG 7 cut(s) 453, 489, 853, 889, 1177, 2104, 2341
BspEI TCCGGA 1 cut(s) 2010
BspFNI CGCG 2 cut(s) 256, 1787
BspHI TCATGA 2 cut(s) 204, 346
BspMI ACCTGC 2 cut(s) 169, 186
BspPI GGATC 9 cut(s) 755, 770, 890, 1721, 2124, 2197, 2516, 2529, 2551
BspQI GCTCTTC 1 cut(s) 129
BspT104I TTCGAA 1 cut(s) 1686
BspTNI GGTCTC 3 cut(s) 55, 1958, 1989
BsrDI GCAATG 3 cut(s) 802, 924, 1620
BsrFI RCCGGY 1 cut(s) 139
BsrGI TGTACA 1 cut(s) 2483
BsrI ACTGG 5 cut(s) 57, 872, 1421, 1583, 2023
BssAI RCCGGY 1 cut(s) 139
BssNAI GTATAC 1 cut(s) 654
BssNI GRCGYC 1 cut(s) 2310
BssSI CACGAG 1 cut(s) 1664
BssT1I CCWWGG 4 cut(s) 743, 954, 988, 2190
Bst1107I GTATAC 1 cut(s) 654
Bst2BI CACGAG 1 cut(s) 1664
Bst2UI CCWGG 4 cut(s) 1544, 1811, 2316, 2436
Bst4CI ACNGT 9 cut(s) 112, 124, 265, 809, 1336, 1447, 1744, 2212, 2488
Bst6I CTCTTC 2 cut(s) 129, 2451
BstACI GRCGYC 1 cut(s) 2310
BstAPI GCANNNNNTGC 1 cut(s) 92
BstAUI TGTACA 1 cut(s) 2483
BstBAI YACGTR 1 cut(s) 565
BstBI TTCGAA 1 cut(s) 1686
BstC8I GCNNGC 7 cut(s) 669, 723, 740, 826, 2287, 2308, 2377
BstDSI CCRYGG 5 cut(s) 254, 475, 743, 988, 1335
BstF5I GGATG 6 cut(s) 106, 456, 1526, 2019, 2079, 2251
BstFNI CGCG 2 cut(s) 256, 1787
BstH2I RGCGCY 1 cut(s) 722
BstHHI GCGC 3 cut(s) 721, 727, 2399
BstMAI GTCTC 5 cut(s) 55, 807, 1431, 1958, 1989
BstMCI CGRYCG 1 cut(s) 1446
BstMWI GCNNNNNNNGC 6 cut(s) 92, 95, 716, 844, 1081, 1235
BstNI CCWGG 4 cut(s) 1544, 1811, 2316, 2436
BstNSI RCATGY 2 cut(s) 2403, 2484
BstPAI GACNNNNGTC 1 cut(s) 1337
BstSCI CCNGG 6 cut(s) 1207, 1320, 1542, 1809, 2314, 2434
BstSFI CTRYAG 1 cut(s) 1935
BstUI CGCG 2 cut(s) 256, 1787
BstV1I GCAGC 4 cut(s) 82, 98, 719, 1387
BstV2I GAAGAC 3 cut(s) 927, 1333, 1675
BstX2I RGATCY 3 cut(s) 2129, 2202, 2521
BstYI RGATCY 3 cut(s) 2129, 2202, 2521
BstZ17I GTATAC 1 cut(s) 654
Bsu36I CCTNAGG 2 cut(s) 440, 1100
BsuRI GGCC 4 cut(s) 277, 451, 828, 1207
BtgI CCRYGG 5 cut(s) 254, 475, 743, 988, 1335
BtsCI GGATG 6 cut(s) 106, 456, 1526, 2019, 2079, 2251
BtsI GCAGTG 2 cut(s) 707, 1727
BtsIMutI CAGTG 5 cut(s) 117, 707, 805, 1727, 1749
BveI ACCTGC 2 cut(s) 169, 186
Cac8I GCNNGC 7 cut(s) 669, 723, 740, 826, 2287, 2308, 2377
CaiI CAGNNNCTG 1 cut(s) 92
CciI TCATGA 2 cut(s) 204, 346
CfoI GCGC 3 cut(s) 721, 727, 2399
Cfr10I RCCGGY 1 cut(s) 139
Cfr13I GGNCC 8 cut(s) 198, 449, 620, 687, 827, 1206, 1547, 1790
Cfr42I CCGCGG 1 cut(s) 257
CseI GACGC 1 cut(s) 2299
Csp6I GTAC 6 cut(s) 880, 985, 1963, 2116, 2241, 2484
CviQI GTAC 6 cut(s) 880, 985, 1963, 2116, 2241, 2484
DraIII CACNNNGTG 1 cut(s) 617
Eam1104I CTCTTC 2 cut(s) 129, 2451
EarI CTCTTC 2 cut(s) 129, 2451
Eco130I CCWWGG 4 cut(s) 743, 954, 988, 2190
Eco24I GRGCYC 1 cut(s) 354
Eco31I GGTCTC 3 cut(s) 55, 1958, 1989
Eco47I GGWCC 5 cut(s) 198, 620, 687, 1547, 1790
Eco57I CTGAAG 3 cut(s) 308, 2007, 2412
Eco81I CCTNAGG 2 cut(s) 440, 1100
EcoO109I RGGNCCY 3 cut(s) 449, 827, 1790
EcoRII CCWGG 4 cut(s) 1542, 1809, 2314, 2434
EcoT14I CCWWGG 4 cut(s) 743, 954, 988, 2190
EcoT22I ATGCAT 1 cut(s) 2253
EcoT38I GRGCYC 1 cut(s) 354
ErhI CCWWGG 4 cut(s) 743, 954, 988, 2190
FalI AAGNNNNNCTT 4 cut(s) 1255, 1287, 2495, 2527
FaqI GGGAC 7 cut(s) 700, 1118, 1761, 1803, 1827, 2297, 2474
FauI CCCGC 2 cut(s) 294, 1780
FauNDI CATATG 2 cut(s) 187, 1552
FbaI TGATCA 1 cut(s) 207
FblI GTMKAC 1 cut(s) 653
Fnu4HI GCNGC 5 cut(s) 87, 96, 708, 1401, 2066
FokI GGATG 6 cut(s) 113, 443, 1533, 2026, 2086, 2238
FriOI GRGCYC 1 cut(s) 354
Fsp4HI GCNGC 5 cut(s) 87, 96, 708, 1401, 2066
FspBI CTAG 4 cut(s) 410, 1256, 1533, 2493
FspI TGCGCA 1 cut(s) 726
GlaI GCGC 3 cut(s) 720, 726, 2398
GluI GCNGC 5 cut(s) 87, 96, 708, 1401, 2066
GsuI CTGGAG 3 cut(s) 1179, 1413, 2418
HaeII RGCGCY 1 cut(s) 722
HaeIII GGCC 4 cut(s) 277, 451, 828, 1207
HapII CCGG 5 cut(s) 140, 1209, 1322, 1824, 2011
HgaI GACGC 1 cut(s) 2299
HhaI GCGC 3 cut(s) 721, 727, 2399
Hin1I GRCGYC 1 cut(s) 2310
Hin6I GCGC 3 cut(s) 719, 725, 2397
HinP1I GCGC 3 cut(s) 719, 725, 2397
HincII GTYRAC 1 cut(s) 2215
HindII GTYRAC 1 cut(s) 2215
HindIII AAGCTT 1 cut(s) 2377
HinfI GANTC 7 cut(s) 319, 1093, 1123, 1465, 1571, 1593, 1749
HpaII CCGG 5 cut(s) 140, 1209, 1322, 1824, 2011
HphI GGTGA 9 cut(s) 193, 406, 427, 905, 1088, 1519, 1646, 1888, 2056
Hpy166II GTNNAC 6 cut(s) 654, 1527, 1652, 1896, 2215, 2243
Hpy188I TCNGA 4 cut(s) 288, 529, 1443, 1592
Hpy8I GTNNAC 6 cut(s) 654, 1527, 1652, 1896, 2215, 2243
HpyCH4III ACNGT 9 cut(s) 112, 124, 265, 809, 1336, 1447, 1744, 2212, 2488
HpyCH4IV ACGT 5 cut(s) 315, 330, 564, 1806, 2262
HpyF10VI GCNNNNNNNGC 6 cut(s) 92, 95, 716, 844, 1081, 1235
HpySE526I ACGT 5 cut(s) 315, 330, 564, 1806, 2262
Hsp92I GRCGYC 1 cut(s) 2310
HspAI GCGC 3 cut(s) 719, 725, 2397
KflI GGGWCCC 1 cut(s) 1790
Kpn2I TCCGGA 1 cut(s) 2010
Ksp22I TGATCA 1 cut(s) 207
KspI CCGCGG 1 cut(s) 257
LguI GCTCTTC 1 cut(s) 129
Lsp1109I GCAGC 4 cut(s) 82, 98, 719, 1387
LweI GCATC 6 cut(s) 1495, 1969, 2067, 2238, 2260, 2419
MaeI CTAG 4 cut(s) 410, 1256, 1533, 2493
MaeII ACGT 5 cut(s) 315, 330, 564, 1806, 2262
MfeI CAATTG 1 cut(s) 1830
MflI RGATCY 3 cut(s) 2129, 2202, 2521
MhlI GDGCHC 4 cut(s) 263, 354, 1779, 1912
MluCI AATT 8 cut(s) 243, 370, 691, 1345, 1372, 1437, 1830, 2152
MlyI GAGTC 2 cut(s) 1102, 1743
MmeI TCCRAC 2 cut(s) 127, 1466
Mph1103I ATGCAT 1 cut(s) 2253
MroI TCCGGA 1 cut(s) 2010
MroXI GAANNNNTTC 1 cut(s) 1437
MseI TTAA 4 cut(s) 786, 1539, 2054, 2265
MslI CAYNNNNRTG 2 cut(s) 737, 1742
MspA1I CMGCKG 1 cut(s) 256
MspI CCGG 5 cut(s) 140, 1209, 1322, 1824, 2011
MspR9I CCNGG 6 cut(s) 1209, 1322, 1544, 1811, 2316, 2436
MunI CAATTG 1 cut(s) 1830
Mva1269I GAATGC 2 cut(s) 772, 2270
MvaI CCWGG 4 cut(s) 1544, 1811, 2316, 2436
MvnI CGCG 2 cut(s) 256, 1787
MwoI GCNNNNNNNGC 6 cut(s) 92, 95, 716, 844, 1081, 1235
NciI CCSGG 2 cut(s) 1209, 1322
NcoI CCATGG 2 cut(s) 743, 988
NdeI CATATG 2 cut(s) 187, 1552
NmeAIII GCCGAG 1 cut(s) 2169
NmuCI GTSAC 9 cut(s) 181, 433, 565, 1094, 1267, 1362, 1607, 1696, 2495
NsbI TGCGCA 1 cut(s) 726
NsiI ATGCAT 1 cut(s) 2253
NspI RCATGY 2 cut(s) 2403, 2484
NspV TTCGAA 1 cut(s) 1686
PagI TCATGA 2 cut(s) 204, 346
PaqCI CACCTGC 1 cut(s) 169
PciI ACATGT 1 cut(s) 2480
PciSI GCTCTTC 1 cut(s) 129
PcsI WCGNNNNNNNCGW 1 cut(s) 1617
PctI GAATGC 2 cut(s) 772, 2270
PdmI GAANNNNTTC 1 cut(s) 1437
PfeI GAWTC 5 cut(s) 319, 1123, 1465, 1571, 1593
PflMI CCANNNNNTGG 3 cut(s) 617, 1604, 1760
PfoI TCCNGGA 2 cut(s) 1320, 2434
PkrI GCNGC 5 cut(s) 88, 97, 709, 1402, 2067
PleI GAGTC 2 cut(s) 1101, 1743
PpsI GAGTC 2 cut(s) 1101, 1743
Ppu21I YACGTR 1 cut(s) 565
PpuMI RGGWCCY 1 cut(s) 1790
PscI ACATGT 1 cut(s) 2480
PshAI GACNNNNGTC 1 cut(s) 1337
PshBI ATTAAT 1 cut(s) 786
Psp5II RGGWCCY 1 cut(s) 1790
Psp6I CCWGG 4 cut(s) 1542, 1809, 2314, 2434
PspGI CCWGG 4 cut(s) 1542, 1809, 2314, 2434
PspPI GGNCC 8 cut(s) 198, 449, 620, 687, 827, 1206, 1547, 1790
PspPPI RGGWCCY 1 cut(s) 1790
PstNI CAGNNNCTG 1 cut(s) 92
PsuI RGATCY 3 cut(s) 2129, 2202, 2521
RsaI GTAC 6 cut(s) 881, 986, 1964, 2117, 2242, 2485
RsaNI GTAC 6 cut(s) 880, 985, 1963, 2116, 2241, 2484
RseI CAYNNNNRTG 2 cut(s) 737, 1742
SacII CCGCGG 1 cut(s) 257
SapI GCTCTTC 1 cut(s) 129
SaqAI TTAA 4 cut(s) 786, 1539, 2054, 2265
SatI GCNGC 5 cut(s) 87, 96, 708, 1401, 2066
Sau96I GGNCC 8 cut(s) 198, 449, 620, 687, 827, 1206, 1547, 1790
SchI GAGTC 2 cut(s) 1102, 1743
ScrFI CCNGG 6 cut(s) 1209, 1322, 1544, 1811, 2316, 2436
SduI GDGCHC 4 cut(s) 263, 354, 1779, 1912
SfaNI GCATC 6 cut(s) 1495, 1969, 2067, 2238, 2260, 2419
SfcI CTRYAG 1 cut(s) 1935
Sfr303I CCGCGG 1 cut(s) 257
SfuI TTCGAA 1 cut(s) 1686
SgrBI CCGCGG 1 cut(s) 257
SinI GGWCC 5 cut(s) 198, 620, 687, 1547, 1790
SmiMI CAYNNNNRTG 2 cut(s) 737, 1742
SmlI CTYRAG 2 cut(s) 2106, 2333
SmoI CTYRAG 2 cut(s) 2106, 2333
Sse9I AATT 8 cut(s) 243, 370, 691, 1345, 1372, 1437, 1830, 2152
SsiI CCGC 6 cut(s) 98, 254, 256, 301, 1787, 2065
SspMI CTAG 4 cut(s) 410, 1256, 1533, 2493
StyD4I CCNGG 6 cut(s) 1207, 1320, 1542, 1809, 2314, 2434
StyI CCWWGG 4 cut(s) 743, 954, 988, 2190
TaaI ACNGT 9 cut(s) 112, 124, 265, 809, 1336, 1447, 1744, 2212, 2488
TaiI ACGT 5 cut(s) 318, 333, 567, 1809, 2265
TaqI TCGA 8 cut(s) 375, 943, 1036, 1059, 1376, 1390, 1686, 2274
TasI AATT 8 cut(s) 243, 370, 691, 1345, 1372, 1437, 1830, 2152
TatI WGTACW 3 cut(s) 879, 2240, 2483
TauI GCSGC 1 cut(s) 2068
TfiI GAWTC 5 cut(s) 319, 1123, 1465, 1571, 1593
Tru1I TTAA 4 cut(s) 786, 1539, 2054, 2265
Tru9I TTAA 4 cut(s) 786, 1539, 2054, 2265
TscAI CASTG 5 cut(s) 117, 707, 812, 1734, 1749
TseFI GTSAC 9 cut(s) 181, 433, 565, 1094, 1267, 1362, 1607, 1696, 2495
TseI GCWGC 4 cut(s) 86, 95, 707, 1400
Tsp45I GTSAC 9 cut(s) 181, 433, 565, 1094, 1267, 1362, 1607, 1696, 2495
TspDTI ATGAA 8 cut(s) 236, 335, 391, 445, 593, 602, 1077, 1580
TspGWI ACGGA 3 cut(s) 492, 512, 975
TspRI CASTG 5 cut(s) 117, 707, 812, 1734, 1749
Van91I CCANNNNNTGG 3 cut(s) 617, 1604, 1760
VpaK11BI GGWCC 5 cut(s) 198, 620, 687, 1547, 1790
VspI ATTAAT 1 cut(s) 786
XapI RAATTY 2 cut(s) 370, 2152
XceI RCATGY 2 cut(s) 2403, 2484
XmiI GTMKAC 1 cut(s) 653
XmnI GAANNNNTTC 1 cut(s) 1437
XspI CTAG 4 cut(s) 410, 1256, 1533, 2493
Zsp2I ATGCAT 1 cut(s) 2253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.