FvH4_2g01270

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002763
Physical Location & Seq
Forward (+)
1199112 .. 1204116
5005 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g01270.t1

Sequence Viewer

Length: 2604 bp
ATGGTGGTACCAAGCCAAAGGGTGCTGTTGATTTCTTTTCTACTTTTGTTGGTCTCCACCACATTAGTCCATGCCAAAGGTAACAAGCCGAAGGCACAAGGTGGTGGCGGTGGGAGCCTCAACGGTGATGACGCCGCCGCTGGGGACAATGTAGATGAGACGGATGACCCTGCAACACAGGGAGGAAAGAAGCAGCTTGATCATGGTCCCGTGACGTATGATGCCAGGTCTGTGATCATTAATGGGAAACATCAGCTTCTATTTTCCGGATCAATTCATTATCCTCGTAGCCCGGTGGAAATGTGGCCTGATATTCTGAGGAAGGCTAGACGGGGAGGTTTGAATGTGATTCAAACTTATGTGTTTTGGAACATTCATGAACCTGAGAAAGGCCAGTGGAACTTTGAGGGAAACTATGATTTGGTGAAGTTTATTAGGATGATCCAGGACAATGGAATGTATGCTACCCTCAGGGTTGGACCATTTATCCAAGCAGAATGGAACCATGGAGGTCTCCCATACTGGCTGAGAGAGGTCCCTGACGTCATTTTCCGATCAGATAATGCACCATACAAGAAACACATGGAGGAGTATGTGACCAAGATAATGAAGTTGATGAAGGATAATAAGCTATTTTCTCCACAGGGAGGTCCAATCATCCTAGCTCAGATTGAGAATGAGTACAATGCTATCCAACTTGCATATAGAGAGTTGGGAGATAGCTATGTGCAGTGGGCAGCAAATTTAGCAGTTGGACAGAATATTGGTGTGCCATGGGTCATGTGCAAGCAAGTAGATGCTCCTGATCCAGTGATTAATACTTGCAATGGAAGGCATTGTGGAGATACTTTTGTAGGACCTAACAAACCCAACAAGCCTGCCCTGTGGACTGAAAATTGGACAGCTCAGTACCGAGTATTTGGTGACCCTCCTTCACAAAGGCCAGCAGAAGACATTGCATTTTCTGTTGCCAGATTTTTCTCCAAGGGTGGATCTCTAACCAACTACTATATGTACCATGGTGGAACAAATTTTGGAAGAACAAGTGCTGTTTTCACAACAACTCGCTACTACGATGAGGCTCCTCTGGATGAATATGCATTCCAAAGGGAACCGAAATGGAGTCACCTCAAAGACTTGCACAAGTCTATAAATCTATGCAAGAAGACTTTGCTTACTGGAATTCCCGGAACACAAAGGTTGGGGCCAGATACTGAGGTTCGTTTCTATGAGAAGCCAGGAACAGAATGTGCTGCTTTTATAGCCAATAATCACACGACCTTGGAAGCAACAGTAAATTGGAGGGGACAGAACTATCTCCTGCCACCATTTTCCATTAGTGTCCTCCCTGACTGCAAGACTGTGGTCTTTAATACTCAACAGATTGTTTCCCAACATAATGCTAGGAACTTTGTTACGTCTGCTAAGCAAATGAACTGGGAAATGTATCTTGAACCCGTTGCAACTGTGGAGCAAGTCCCAGTCAATAATAGGGAACTACAGGAGCTCTATGGCTTGCTAAAAGATAAAACAGATTATGCATGGTACACAACTAGTATTGAGCTAGGTCCATATGACTTGCCAATGAAGGACACTATCAGCCCAGTGGTACGAATTCCTAGTCTCGGTCATGCAATGCTTGTGTTTGTCAATGATGAATATGCTGGATCCCAACACGGTACCCACGCAGAGAAAGGTTTCGTGTTCGAGGCACCTGTTAAAATGAAGAAAGGGACGAACCATATCTCTCTGCTTTGCATGACAGTGGGACTTCCAGATAGTGGAGCATACATGGAGCACAGGTATGCAGGGCCTCGTTCTGTAACAATCCTAGGATTGAACACCGGAACAATTGACGTGTCTGCAAATGGCTGGGGACATAGGGCTGGCCTAAATGGTGAGAAGCTCCAAGTGTTCACTGAACAGGGAGCTCAGAAAGTGCAATGGACTAAGGCAACAGGTATAGGAAGGGCTCTGACATGGTACAAGACAACTTTTGATGCTCCTCCAGGAGGAGACCCTTGTGCTATCCGAATGACCGGTATGGGCAAGGGAATGATTTGGGTGAATGGTATAAGCATCGGGCGTCATTGGATGTCCTTCCTCTCTCCTCTTGGACAACCTACTCAAACCGAGTACCACATCCCTAGAGCCTTCATCAAGCCTAAGAATCTCCTTGTTGTATTGGAGGAGGAGCCATCAGACCCGAAAGACATTGAGATCGTAACAGTAAACAGAGACACCATATGCAGCCTTATTGGACAGGACTATCCGGGACATGTTAAGTACTGGGACAGAAAGGCTGGCATCCTCCAACATGCTCCTGGGAAAAGTCTCCAAGCAACAGCTCTCCTCAACTGTCCAGACTACAAAAAGATTAGTAAAATTGAATTCGCGAGCTACGGCAATCCTGATGGTTCCTGTGGAGCCTTGACAATGGGAACATGCAATGCTCCTGAATCCAAGAAGGTCGTCGAAGATCTATGCTTGGGAAAGATGATATGTACAGTCCCACTTGACCCCAAACTCTTCTTCAAGGCTGGTGATCCCTGCCCTGGTACCAAGAAGAAACTAATGATCCAAGCATTGTGCAGCTTCAAGTGA

Protein Analysis

868

Amino Acids

96.47

Weight (kDa)

8.27

Isoelectric Point (pI)

37.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 78 - 383 3.4e-114 Glycosyl hydrolases family 35
Glyco_hydro_42 PF02449 98 - 230 8.7e-07 Beta-galactosidase
GHD PF17834 407 - 462 3.4e-19 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 659 - 723 1.3e-21 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 785 - 864 6.6e-17 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 546
Acc65I GGTACC 3 cut(s) 7, 1679, 2558
AccB1I GGYRCC 4 cut(s) 7, 1679, 1711, 2558
AccB7I CCANNNNNTGG 1 cut(s) 1781
AccII CGCG 1 cut(s) 2396
AccIII TCCGGA 1 cut(s) 266
AciI CCGC 3 cut(s) 108, 135, 138
AclWI GGATC 8 cut(s) 277, 436, 800, 1000, 1662, 1675, 2540, 2572
AcsI RAATTY 5 cut(s) 742, 1030, 1182, 1614, 2390
AcyI GRCGYC 3 cut(s) 132, 543, 2086
AdeI CACNNNGTG 1 cut(s) 101
AfiI CCNNNNNNNGG 7 cut(s) 141, 389, 647, 1625, 1781, 2012, 2555
AflIII ACRYGT 2 cut(s) 1857, 2278
AgeI ACCGGT 1 cut(s) 2039
AgsI TTSAA 7 cut(s) 343, 353, 1454, 1840, 2390, 2536, 2599
AhlI ACTAGT 1 cut(s) 1553
AjiI CACGTC 1 cut(s) 1858
AjnI CCWGG 6 cut(s) 224, 444, 1237, 2008, 2323, 2554
AjuI GAANNNNNNNTTGG 2 cut(s) 2516, 2548
Alw21I GWGCWC 3 cut(s) 1509, 1800, 1933
Alw26I GTCTC 7 cut(s) 58, 152, 518, 1628, 2010, 2232, 2339
AlwI GGATC 8 cut(s) 277, 436, 800, 1000, 1662, 1675, 2540, 2572
AlwNI CAGNNNCTG 1 cut(s) 1214
Aor13HI TCCGGA 1 cut(s) 266
AoxI GGCC 6 cut(s) 305, 391, 941, 1205, 1811, 1888
ApeKI GCWGC 5 cut(s) 193, 737, 1253, 2250, 2592
ApoI RAATTY 5 cut(s) 742, 1030, 1182, 1614, 2390
ArsI GACNNNNNNTTYG 2 cut(s) 1606, 1638
AseI ATTAAT 2 cut(s) 240, 816
AsiGI ACCGGT 1 cut(s) 2039
Asp700I GAANNNNTTC 1 cut(s) 1697
Asp718I GGTACC 3 cut(s) 7, 1679, 2558
AspA2I CCTAGG 1 cut(s) 1831
AspS9I GGNCC 8 cut(s) 206, 479, 535, 650, 857, 1205, 1568, 1811
AsuC2I CCSGG 3 cut(s) 293, 1188, 2274
AsuHPI GGTGA 7 cut(s) 137, 436, 935, 1118, 1910, 2077, 2555
AvaII GGWCC 6 cut(s) 206, 479, 535, 650, 857, 1568
AvrII CCTAGG 1 cut(s) 1831
AxyI CCTNAGG 1 cut(s) 470
BaeI ACNNNNGTAYC 4 cut(s) 837, 870, 1203, 1236
BamHI GGATCC 1 cut(s) 1667
BanI GGYRCC 4 cut(s) 7, 1679, 1711, 2558
BanII GRGCYC 3 cut(s) 1509, 1933, 1975
BbsI GAAGAC 2 cut(s) 957, 1172
Bbv12I GWGCWC 3 cut(s) 1509, 1800, 1933
BbvI GCAGC 4 cut(s) 205, 749, 1240, 2262
BccI CCATC 2 cut(s) 2206, 2408
BceAI ACGGC 1 cut(s) 2419
BciT130I CCWGG 6 cut(s) 226, 446, 1239, 2010, 2325, 2556
BclI TGATCA 2 cut(s) 199, 234
BcnI CCSGG 3 cut(s) 293, 1188, 2274
BcoDI GTCTC 7 cut(s) 58, 152, 518, 1628, 2010, 2232, 2339
BcuI ACTAGT 1 cut(s) 1553
BfaI CTAG 8 cut(s) 327, 662, 1404, 1554, 1565, 1620, 1832, 2148
BfmI CTRYAG 1 cut(s) 1499
BglII AGATCT 1 cut(s) 2479
BisI GCNGC 7 cut(s) 135, 138, 194, 738, 1254, 2251, 2593
BlnI CCTAGG 1 cut(s) 1831
BlpI GCTNAGC 1 cut(s) 1425
BlsI GCNGC 7 cut(s) 136, 139, 195, 739, 1255, 2252, 2594
BmcAI AGTACT 1 cut(s) 2288
Bme1390I CCNGG 9 cut(s) 226, 293, 446, 1188, 1239, 2010, 2274, 2325, 2556
Bme18I GGWCC 6 cut(s) 206, 479, 535, 650, 857, 1568
BmgBI CACGTC 1 cut(s) 1858
BmgT120I GGNCC 8 cut(s) 206, 479, 535, 650, 857, 1205, 1568, 1811
BmrFI CCNGG 9 cut(s) 226, 293, 446, 1188, 1239, 2010, 2274, 2325, 2556
BmrI ACTGGG 4 cut(s) 1447, 1475, 1598, 2299
BmsI GCATC 5 cut(s) 211, 787, 1990, 2088, 2316
BmuI ACTGGG 4 cut(s) 1447, 1475, 1598, 2299
BoxI GACNNNNGTC 1 cut(s) 1364
BpiI GAAGAC 2 cut(s) 957, 1172
BpmI CTGGAG 1 cut(s) 1992
Bpu1102I GCTNAGC 1 cut(s) 1425
BpuMI CCSGG 3 cut(s) 293, 1188, 2274
BsaHI GRCGYC 3 cut(s) 132, 543, 2086
BsaI GGTCTC 3 cut(s) 58, 518, 2010
BsaJI CCNNGG 8 cut(s) 505, 773, 984, 1018, 1281, 1831, 2324, 2554
BsaWI WCCGGW 3 cut(s) 266, 1844, 2039
BsaXI ACNNNNNCTCC 2 cut(s) 1787, 1817
Bsc4I CCNNNNNNNGG 7 cut(s) 141, 389, 647, 1625, 1781, 2012, 2555
Bse118I RCCGGY 1 cut(s) 2039
Bse1I ACTGG 8 cut(s) 394, 527, 809, 1183, 1442, 1481, 1604, 2294
Bse21I CCTNAGG 1 cut(s) 470
Bse3DI GCAATG 5 cut(s) 832, 954, 1641, 1949, 2455
BseAI TCCGGA 1 cut(s) 266
BseBI CCWGG 6 cut(s) 226, 446, 1239, 2010, 2325, 2556
BseDI CCNNGG 8 cut(s) 505, 773, 984, 1018, 1281, 1831, 2324, 2554
BseGI GGATG 7 cut(s) 169, 444, 657, 1096, 2100, 2142, 2307
BseLI CCNNNNNNNGG 7 cut(s) 141, 389, 647, 1625, 1781, 2012, 2555
BseMI GCAATG 5 cut(s) 832, 954, 1641, 1949, 2455
BseMII CTCAG 8 cut(s) 308, 375, 484, 518, 680, 920, 1206, 1946
BseNI ACTGG 8 cut(s) 394, 527, 809, 1183, 1442, 1481, 1604, 2294
BseRI GAGGAG 8 cut(s) 602, 1074, 1995, 2028, 2100, 2204, 2207, 2342
BseXI GCAGC 4 cut(s) 205, 749, 1240, 2262
BseYI CCCAGC 2 cut(s) 140, 1872
BsgI GTGCAG 1 cut(s) 749
Bsh1236I CGCG 1 cut(s) 2396
BshFI GGCC 6 cut(s) 307, 393, 943, 1207, 1813, 1890
BshNI GGYRCC 4 cut(s) 7, 1679, 1711, 2558
BshTI ACCGGT 1 cut(s) 2039
BsiHKAI GWGCWC 3 cut(s) 1509, 1800, 1933
BsiSI CCGG 6 cut(s) 267, 293, 1188, 1845, 2040, 2273
BslI CCNNNNNNNGG 7 cut(s) 141, 389, 647, 1625, 1781, 2012, 2555
BsmAI GTCTC 7 cut(s) 58, 152, 518, 1628, 2010, 2232, 2339
BsmBI CGTCTC 1 cut(s) 152
BsmI GAATGC 1 cut(s) 1100
BsnI GGCC 6 cut(s) 307, 393, 943, 1207, 1813, 1890
Bso31I GGTCTC 3 cut(s) 58, 518, 2010
Bsp1286I GDGCHC 4 cut(s) 1509, 1800, 1933, 1975
Bsp13I TCCGGA 1 cut(s) 266
Bsp1407I TGTACA 1 cut(s) 2504
Bsp1720I GCTNAGC 1 cut(s) 1425
Bsp19I CCATGG 3 cut(s) 505, 773, 1018
Bsp68I TCGCGA 1 cut(s) 2396
BspACI CCGC 3 cut(s) 108, 135, 138
BspANI GGCC 6 cut(s) 307, 393, 943, 1207, 1813, 1890
BspCNI CTCAG 8 cut(s) 309, 376, 483, 519, 679, 919, 1207, 1945
BspEI TCCGGA 1 cut(s) 266
BspFNI CGCG 1 cut(s) 2396
BspHI TCATGA 1 cut(s) 376
BspPI GGATC 8 cut(s) 277, 436, 800, 1000, 1662, 1675, 2540, 2572
BspT107I GGYRCC 4 cut(s) 7, 1679, 1711, 2558
BspTNI GGTCTC 3 cut(s) 58, 518, 2010
BsrDI GCAATG 5 cut(s) 832, 954, 1641, 1949, 2455
BsrFI RCCGGY 1 cut(s) 2039
BsrGI TGTACA 1 cut(s) 2504
BsrI ACTGG 8 cut(s) 394, 527, 809, 1183, 1442, 1481, 1604, 2294
BssAI RCCGGY 1 cut(s) 2039
BssECI CCNNGG 8 cut(s) 505, 773, 984, 1018, 1281, 1831, 2324, 2554
BssNI GRCGYC 3 cut(s) 132, 543, 2086
BssT1I CCWWGG 6 cut(s) 505, 773, 984, 1018, 1281, 1831
Bst2UI CCWGG 6 cut(s) 226, 446, 1239, 2010, 2325, 2556
Bst4CI ACNGT 9 cut(s) 125, 1294, 1363, 1468, 1679, 1765, 2230, 2360, 2509
Bst6I CTCTTC 1 cut(s) 2534
BstACI GRCGYC 3 cut(s) 132, 543, 2086
BstAUI TGTACA 1 cut(s) 2504
BstC8I GCNNGC 7 cut(s) 788, 879, 945, 1517, 1888, 2305, 2398
BstDSI CCRYGG 3 cut(s) 505, 773, 1018
BstEII GGTNACC 1 cut(s) 923
BstENI CCTNNNNNAGG 2 cut(s) 387, 2010
BstF5I GGATG 7 cut(s) 169, 444, 657, 1096, 2100, 2142, 2307
BstFNI CGCG 1 cut(s) 2396
BstMAI GTCTC 7 cut(s) 58, 152, 518, 1628, 2010, 2232, 2339
BstMWI GCNNNNNNNGC 3 cut(s) 114, 746, 1262
BstNI CCWGG 6 cut(s) 226, 446, 1239, 2010, 2325, 2556
BstNSI RCATGY 3 cut(s) 2282, 2321, 2448
BstPAI GACNNNNGTC 1 cut(s) 1364
BstPI GGTNACC 1 cut(s) 923
BstSCI CCNGG 9 cut(s) 224, 291, 444, 1186, 1237, 2008, 2272, 2323, 2554
BstSFI CTRYAG 1 cut(s) 1499
BstUI CGCG 1 cut(s) 2396
BstV1I GCAGC 4 cut(s) 205, 749, 1240, 2262
BstV2I GAAGAC 2 cut(s) 957, 1172
BstX2I RGATCY 3 cut(s) 992, 1667, 2479
BstXI CCANNNNNNTGG 1 cut(s) 452
BstYI RGATCY 3 cut(s) 992, 1667, 2479
Bsu36I CCTNAGG 1 cut(s) 470
BsuRI GGCC 6 cut(s) 307, 393, 943, 1207, 1813, 1890
BtgI CCRYGG 3 cut(s) 505, 773, 1018
BtrI CACGTC 1 cut(s) 1858
BtsCI GGATG 7 cut(s) 169, 444, 657, 1096, 2100, 2142, 2307
BtsI GCAGTG 1 cut(s) 737
BtsIMutI CAGTG 6 cut(s) 401, 737, 816, 1611, 1770, 1917
BtuMI TCGCGA 1 cut(s) 2396
Cac8I GCNNGC 7 cut(s) 788, 879, 945, 1517, 1888, 2305, 2398
CaiI CAGNNNCTG 1 cut(s) 1214
CciI TCATGA 1 cut(s) 376
Cfr10I RCCGGY 1 cut(s) 2039
Cfr13I GGNCC 8 cut(s) 206, 479, 535, 650, 857, 1205, 1568, 1811
CseI GACGC 2 cut(s) 140, 2075
CspAI ACCGGT 1 cut(s) 2039
DraIII CACNNNGTG 1 cut(s) 101
Eam1104I CTCTTC 1 cut(s) 2534
EarI CTCTTC 1 cut(s) 2534
Ecl136II GAGCTC 2 cut(s) 1507, 1931
Eco130I CCWWGG 6 cut(s) 505, 773, 984, 1018, 1281, 1831
Eco24I GRGCYC 3 cut(s) 1509, 1933, 1975
Eco31I GGTCTC 3 cut(s) 58, 518, 2010
Eco47I GGWCC 6 cut(s) 206, 479, 535, 650, 857, 1568
Eco53kI GAGCTC 2 cut(s) 1507, 1931
Eco81I CCTNAGG 1 cut(s) 470
Eco91I GGTNACC 1 cut(s) 923
EcoICRI GAGCTC 2 cut(s) 1507, 1931
EcoNI CCTNNNNNAGG 2 cut(s) 387, 2010
EcoO109I RGGNCCY 3 cut(s) 535, 857, 1811
EcoO65I GGTNACC 1 cut(s) 923
EcoRI GAATTC 3 cut(s) 1182, 1614, 2390
EcoRII CCWGG 6 cut(s) 224, 444, 1237, 2008, 2323, 2554
EcoT14I CCWWGG 6 cut(s) 505, 773, 984, 1018, 1281, 1831
EcoT22I ATGCAT 2 cut(s) 1102, 1543
EcoT38I GRGCYC 3 cut(s) 1509, 1933, 1975
ErhI CCWWGG 6 cut(s) 505, 773, 984, 1018, 1281, 1831
Esp3I CGTCTC 1 cut(s) 152
FauNDI CATATG 2 cut(s) 1573, 2246
FbaI TGATCA 2 cut(s) 199, 234
Fnu4HI GCNGC 7 cut(s) 135, 138, 194, 738, 1254, 2251, 2593
FokI GGATG 7 cut(s) 176, 451, 644, 1103, 2107, 2129, 2294
FriOI GRGCYC 3 cut(s) 1509, 1933, 1975
Fsp4HI GCNGC 7 cut(s) 135, 138, 194, 738, 1254, 2251, 2593
FspBI CTAG 8 cut(s) 327, 662, 1404, 1554, 1565, 1620, 1832, 2148
GluI GCNGC 7 cut(s) 135, 138, 194, 738, 1254, 2251, 2593
GsaI CCCAGC 2 cut(s) 144, 1876
GsuI CTGGAG 1 cut(s) 1992
HaeIII GGCC 6 cut(s) 307, 393, 943, 1207, 1813, 1890
HapII CCGG 6 cut(s) 267, 293, 1188, 1845, 2040, 2273
HgaI GACGC 2 cut(s) 140, 2075
Hin1I GRCGYC 3 cut(s) 132, 543, 2086
HinfI GANTC 4 cut(s) 349, 1123, 2170, 2459
HpaII CCGG 6 cut(s) 267, 293, 1188, 1845, 2040, 2273
HphI GGTGA 7 cut(s) 137, 436, 935, 1118, 1910, 2077, 2555
Hpy166II GTNNAC 4 cut(s) 888, 1548, 1917, 2233
Hpy188I TCNGA 8 cut(s) 318, 554, 559, 669, 1935, 1977, 2033, 2203
Hpy8I GTNNAC 4 cut(s) 888, 1548, 1917, 2233
Hpy99I CGWCG 1 cut(s) 2477
HpyCH4III ACNGT 9 cut(s) 125, 1294, 1363, 1468, 1679, 1765, 2230, 2360, 2509
HpyCH4IV ACGT 4 cut(s) 215, 543, 1418, 1857
HpyF10VI GCNNNNNNNGC 3 cut(s) 114, 746, 1262
HpySE526I ACGT 4 cut(s) 215, 543, 1418, 1857
Hsp92I GRCGYC 3 cut(s) 132, 543, 2086
Kpn2I TCCGGA 1 cut(s) 266
KpnI GGTACC 3 cut(s) 11, 1683, 2562
Ksp22I TGATCA 2 cut(s) 199, 234
Lsp1109I GCAGC 4 cut(s) 205, 749, 1240, 2262
LweI GCATC 5 cut(s) 211, 787, 1990, 2088, 2316
MaeI CTAG 8 cut(s) 327, 662, 1404, 1554, 1565, 1620, 1832, 2148
MaeII ACGT 4 cut(s) 215, 543, 1418, 1857
MaeIII GTNAC 8 cut(s) 80, 211, 595, 923, 1124, 1414, 1822, 2224
MboII GAAGA 8 cut(s) 962, 1050, 1177, 1738, 2489, 2521, 2524, 2578
MfeI CAATTG 1 cut(s) 1851
MflI RGATCY 3 cut(s) 992, 1667, 2479
MhlI GDGCHC 4 cut(s) 1509, 1800, 1933, 1975
MlyI GAGTC 1 cut(s) 1132
MmeI TCCRAC 4 cut(s) 457, 718, 733, 2338
Mph1103I ATGCAT 2 cut(s) 1102, 1543
MroI TCCGGA 1 cut(s) 266
MroXI GAANNNNTTC 1 cut(s) 1697
MseI TTAA 5 cut(s) 240, 816, 1371, 1719, 2283
MslI CAYNNNNRTG 2 cut(s) 1763, 1803
MspA1I CMGCKG 1 cut(s) 140
MspI CCGG 6 cut(s) 267, 293, 1188, 1845, 2040, 2273
MspR9I CCNGG 9 cut(s) 226, 293, 446, 1188, 1239, 2010, 2274, 2325, 2556
MunI CAATTG 1 cut(s) 1851
Mva1269I GAATGC 1 cut(s) 1100
MvaI CCWGG 6 cut(s) 226, 446, 1239, 2010, 2325, 2556
MvnI CGCG 1 cut(s) 2396
MwoI GCNNNNNNNGC 3 cut(s) 114, 746, 1262
NciI CCSGG 3 cut(s) 293, 1188, 2274
NcoI CCATGG 3 cut(s) 505, 773, 1018
NdeI CATATG 2 cut(s) 1573, 2246
NmuCI GTSAC 4 cut(s) 211, 595, 923, 1124
NruI TCGCGA 1 cut(s) 2396
NsiI ATGCAT 2 cut(s) 1102, 1543
NspI RCATGY 3 cut(s) 2282, 2321, 2448
PagI TCATGA 1 cut(s) 376
PciI ACATGT 1 cut(s) 2278
PctI GAATGC 1 cut(s) 1100
PdmI GAANNNNTTC 1 cut(s) 1697
PfeI GAWTC 3 cut(s) 349, 2170, 2459
PflMI CCANNNNNTGG 1 cut(s) 1781
PfoI TCCNGGA 4 cut(s) 444, 1186, 2008, 2272
PinAI ACCGGT 1 cut(s) 2039
PkrI GCNGC 7 cut(s) 136, 139, 195, 739, 1255, 2252, 2594
PleI GAGTC 1 cut(s) 1131
PpsI GAGTC 1 cut(s) 1131
PpuMI RGGWCCY 2 cut(s) 535, 857
PscI ACATGT 1 cut(s) 2278
PshAI GACNNNNGTC 1 cut(s) 1364
PshBI ATTAAT 2 cut(s) 240, 816
Psp124BI GAGCTC 2 cut(s) 1509, 1933
Psp5II RGGWCCY 2 cut(s) 535, 857
Psp6I CCWGG 6 cut(s) 224, 444, 1237, 2008, 2323, 2554
PspEI GGTNACC 1 cut(s) 923
PspFI CCCAGC 2 cut(s) 140, 1872
PspGI CCWGG 6 cut(s) 224, 444, 1237, 2008, 2323, 2554
PspPI GGNCC 8 cut(s) 206, 479, 535, 650, 857, 1205, 1568, 1811
PspPPI RGGWCCY 2 cut(s) 535, 857
PstNI CAGNNNCTG 1 cut(s) 1214
PsuI RGATCY 3 cut(s) 992, 1667, 2479
RruI TCGCGA 1 cut(s) 2396
RseI CAYNNNNRTG 2 cut(s) 1763, 1803
SacI GAGCTC 2 cut(s) 1509, 1933
SaqAI TTAA 5 cut(s) 240, 816, 1371, 1719, 2283
SatI GCNGC 7 cut(s) 135, 138, 194, 738, 1254, 2251, 2593
Sau96I GGNCC 8 cut(s) 206, 479, 535, 650, 857, 1205, 1568, 1811
ScaI AGTACT 1 cut(s) 2288
SchI GAGTC 1 cut(s) 1132
ScrFI CCNGG 9 cut(s) 226, 293, 446, 1188, 1239, 2010, 2274, 2325, 2556
SduI GDGCHC 4 cut(s) 1509, 1800, 1933, 1975
SfaNI GCATC 5 cut(s) 211, 787, 1990, 2088, 2316
SfcI CTRYAG 1 cut(s) 1499
SinI GGWCC 6 cut(s) 206, 479, 535, 650, 857, 1568
SmiMI CAYNNNNRTG 2 cut(s) 1763, 1803
SpeI ACTAGT 1 cut(s) 1553
SsiI CCGC 3 cut(s) 108, 135, 138
SspI AATATT 1 cut(s) 763
SspMI CTAG 8 cut(s) 327, 662, 1404, 1554, 1565, 1620, 1832, 2148
SstI GAGCTC 2 cut(s) 1509, 1933
StyD4I CCNGG 9 cut(s) 224, 291, 444, 1186, 1237, 2008, 2272, 2323, 2554
StyI CCWWGG 6 cut(s) 505, 773, 984, 1018, 1281, 1831
TaaI ACNGT 9 cut(s) 125, 1294, 1363, 1468, 1679, 1765, 2230, 2360, 2509
TaiI ACGT 4 cut(s) 218, 546, 1421, 1860
TaqI TCGA 2 cut(s) 1707, 2475
TaqII GACCGA 1 cut(s) 1616
TatI WGTACW 3 cut(s) 681, 2286, 2504
TauI GCSGC 2 cut(s) 137, 140
TfiI GAWTC 3 cut(s) 349, 2170, 2459
Tru1I TTAA 5 cut(s) 240, 816, 1371, 1719, 2283
Tru9I TTAA 5 cut(s) 240, 816, 1371, 1719, 2283
TscAI CASTG 6 cut(s) 401, 737, 816, 1611, 1770, 1924
TseFI GTSAC 4 cut(s) 211, 595, 923, 1124
TseI GCWGC 5 cut(s) 193, 737, 1253, 2250, 2592
Tsp45I GTSAC 4 cut(s) 211, 595, 923, 1124
TspGWI ACGGA 1 cut(s) 176
TspRI CASTG 6 cut(s) 401, 737, 816, 1611, 1770, 1924
Van91I CCANNNNNTGG 1 cut(s) 1781
VpaK11BI GGWCC 6 cut(s) 206, 479, 535, 650, 857, 1568
VspI ATTAAT 2 cut(s) 240, 816
XagI CCTNNNNNAGG 2 cut(s) 387, 2010
XapI RAATTY 5 cut(s) 742, 1030, 1182, 1614, 2390
XceI RCATGY 3 cut(s) 2282, 2321, 2448
XcmI CCANNNNNNNNNTGG 1 cut(s) 2321
XmaJI CCTAGG 1 cut(s) 1831
XmnI GAANNNNTTC 1 cut(s) 1697
XspI CTAG 8 cut(s) 327, 662, 1404, 1554, 1565, 1620, 1832, 2148
ZraI GACGTC 1 cut(s) 544
ZrmI AGTACT 1 cut(s) 2288
Zsp2I ATGCAT 2 cut(s) 1102, 1543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.