Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4C

Orthogroup ID

OG0002763

Physical Location & Seq

Genomic Coordinates Forward (+)

42821616 .. 42824712

Gene Structure 3097 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG202000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2529 bp

ATGGCGGTACCAAGCTACAGGTTGCTCTTAATAGCTCTTGTTTTCTTGGTTGTTTCAGCTGCAGTAGCCCATACGAAAAGCCAAGGTCGTAGCCACGATGGTGGGAAGCACCAGCTTGAAACAGGGCCCGTAACATATGATGGGAGGTCGCTGCTTATCAATGGCAAACGAGAGCTTCTATTTTCGGGTTCAATTCATTATACACGAAGCACGCCAGAAATGTGGGCTGATCTTCTCCGAAAGGCTAAATATGGAGGTTTGAATGTGATTCAAACTTATGTGTTTTGGAACATTCACGAGCCAGTGAAAGGTCAATACAATTTTTCAGGAAACTATGATTTGGTGAGGTTCATTAAGCTTGTTCAAGAGCATGGAATGTATGTTACCCTTCGAGTTGGGCCATTCATTCAAGCCGAATGGAACCATGGAGGACTTCCATATTGGCTGAGAGAGGTCCCCAACCTAATATTTCGTACTGATAATGCACCATACAAGTACTATATGAAGAAGTATGTGACCATGATTGTAAACAAGCTGAAAGAAGCGAAGCTATTTTTTCCTCAAGGTGGCCCAATCATATTGGCACAGATTGAAAATGAGTACGATCATATACAGCTTGCATATAAAGACATGGGATTCAGTTATGTCAGATGGGCAGCAAGGATGGCCGTAAAGCAAAACGCTGGAGTACCATGGATCATGTGCAAGCAGACCGATGCTCCTGATCCGGTGATTAATACTTGTAACGGAAGGCATTGTGGAGATACTTTCTCCGGGCCTAATAAGCCATACAAGCCTGCTCTATGGACCGAAAACTGGACAGCTCAGTACAGAGTATTTGGTGATCCAGCTTCACAGAGACCCGCAGAAGATATTGCATTTTCAGTTGCCCGCTTTTTCTCAAAGAATGGAGCTCTAACCAACTACTACATGTACCATGGTGGGACTAATTTTGGAAGAACAAGTGCGATTTTTACAACAACTCGCTACTATGACGAAGCACCTCTTGACGAATATGGTTTGCCAAGGGACCCAAAATGGAGTCACCTCAAGGACTTGCACAAGGCTTTGAGACTAAGCAGGAAGCCTTTGCTTTGGGGAGTTCCAGGTGTCCAAAACTTAGGGGAAAACCTCGAGGCTCGTTTCTACGAGTTGAAAAACAACTCCAAAATCTGTGCTGCTTTCTTGGCCAACAACAATTCAAGGAGTGAAGCAATTGTTAATTGGCGAGGACAAGACTACTACCTTCCACCGCATTCCATTAGCATCCTCCCTGACTGCAAGACCGTAGTCTACAATACTCAAACCATCGTATCGCAACATAATGCAAGGAACATTGTCAGATCAACTTTGGCCAACAAAATGAGATGGATGGAGTCTTCAGAACCCATTCCAACCACCGTGCAAGTTCCGATCAATAATAGGACACCGTTGGAGCTCTACAGCTTGCTAAAAGATCAGTCAGACTATGCGTGGTACACAACCAGCCTTGAATTGAATCCCAGGGACATGTCCATGAAGGAAAGTATTCAACCGGTTTTACGAATTGGAAGTCTCGGTCACGCAATGCTGGTGTTTGTCAATGGTGAATATGTTGGTTCCGGACATGGGAGCCACGACGAGAAGAGCTTTGTCTTCCAAAAAGTTGTGTCCTTAAAGGCCGGGGTCAACCAAATAGCTTTGCTTGCCATGACAGTTGGTCTTCCAGATAGCGGAGCGTACATGGAGCACAGATATGCAGGGCCTAAAGACGTAGAAGTTCTAGGACTGAACACTGGAACTATAGATCTCTCACAAAATGGGTGGGGGCATCAAGTTGGCCTGAATGGAGAGAAGATCCAAGTCTTCACTGAAGAGGGATCGAAACGGGTTCAATGGGGTGAAATAAATGGACCAAAAAAGGCTCTAACATGGTACAAGACATATTTTGATGCTCCTGAAGGGAATGACCCTGTGGCTATTAAAATGTCTGGTATGGGAAAAGGAATGATGTGGGTGAATGGTGAGAGCATTGGCCGGCACTGGATGTCTTTCCTCTCTCCTCTAGGACAACCTACTCAATCCGAGTACCACATCCCAAGATCCTTCATCAAGCCTACACAAAACCTTCTTATTGTTTTGGAAGAGGAGCCAGCCAAGCCTAATAGCATTGAGATTATGACTGTCAACAGAGATACCATTTGCAGCTTCATTACTGAGTATCATCCTCCAAATGTCAAGTCATGGGCAAGAGAGAACAGCCTCTTCCAACCTGTTTTAGATGTTGTCAGAACATCCGCGGAGTTGAAATGTCCAAACTACAAAAAGGTTGTCGAAGTTGAGTTTGCAAGCTTCGGCGATCCTGTTGGTTCATGTGGAAGCTACACCTTGGGTAAATGCGACTCCCCTGTATCTAAAGAGGTTGTTGAACAGCACTGCTTGAACAAAACTAGCTGCATAGTTCCGATTGACCGCAAGCTTTACTTCAAGAACAATGGTGATTGCCCAGATATCAAGAAAACACTCGCCATCCAAGTGAAGTGTGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BetaGal_dom4_5 Gal_Lectin Glyco_hydro_35

Protein Analysis

842

Amino Acids

95.05

Weight (kDa)

8.87

Isoelectric Point (pI)

38.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	50 - 356	1.4e-116	Glycosyl hydrolases family 35
Glyco_hydro_42	PF02449	72 - 203	1.3e-07	Beta-galactosidase
GHD	PF17834	380 - 436	1.9e-20	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	636 - 702	3.1e-24	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	762 - 841	9.7e-15	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002763)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16730 AT4G35010
fragaria_vesca	FvH4_2g01270 FvH4_4g13900
malus_domestica	MD04G1010100.v1.1 MD05G1121000.v1.1 MD10G1095200.v1.1 MD13G1284600.v1.1
prunus_persica	Prupe.1G009200_v2.0.a1 Prupe.1G009300_v2.0.a1 Prupe.8G164800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0475241 RchiOBHm_Chr4g0415021 RchiOBHm_Chr6g0251931
rosa_laevigata	RLG00000008102 RLG00000015418 RLG00000023857
rosa_multiflora	Rmu_sc0003611.1_g000013 Rmu_sc0008157.1_g000007 Rmu_ssc0000303.1_g000015
rosa_roxburghii	Rroxscaffold_5G00358760 Rroxscaffold_6G00406440
rosa_rugosa	Rorug03G0147300 Rorug04G0131700 Rorug05G0505100 Rorug05G0505200 Rorug05G0505200
rosa_samantha	Rh3AG197300 Rh4AG191000 Rh4BG189100 Rh4CG202000 Rh4DG189500 Rh6AG016400 Rh6BG014400 Rh6CG011300 Rh6DG012600
rosa_wichuraiana	Rw3G017940 Rw4G016250 Rw6G001570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	1133
Acc65I	GGTACC	1 cut(s)	7
AccB1I	GGYRCC	1 cut(s)	7
AccI	GTMKAC	1 cut(s)	1293
AccII	CGCG	1 cut(s)	2279
AccIII	TCCGGA	1 cut(s)	1601
AciI	CCGC	8 cut(s)	5, 864, 892, 1253, 1713, 2277, 2279, 2452
AclWI	GGATC	7 cut(s)	704, 719, 839, 1831, 1867, 2076, 2333
AcoI	YGGCCR	4 cut(s)	666, 1188, 1353, 2014
AcuI	CTGAAG	3 cut(s)	1365, 1872, 1959
AfiI	CCNNNNNNNGG	5 cut(s)	308, 566, 816, 1608, 1712
AflIII	ACRYGT	2 cut(s)	930, 1509
AgeI	ACCGGT	1 cut(s)	1534
AhdI	GACNNNNNGTC	1 cut(s)	1698
AjnI	CCWGG	2 cut(s)	1105, 1502
AleI	CACNNNNGTG	1 cut(s)	99
Alw21I	GWGCWC	3 cut(s)	916, 1440, 1731
Alw26I	GTCTC	3 cut(s)	853, 1066, 1559
AlwI	GGATC	7 cut(s)	704, 719, 839, 1831, 1867, 2076, 2333
Ama87I	CYCGRG	1 cut(s)	1133
Aor13HI	TCCGGA	1 cut(s)	1601
ApaI	GGGCCC	1 cut(s)	129
ApeKI	GCWGC	6 cut(s)	59, 151, 656, 1178, 2184, 2433
ArsI	GACNNNNNNTTYG	6 cut(s)	1537, 1569, 2204, 2236, 2295, 2327
AseI	ATTAAT	1 cut(s)	735
AsiGI	ACCGGT	1 cut(s)	1534
Asp700I	GAANNNNTTC	2 cut(s)	1389, 1527
Asp718I	GGTACC	1 cut(s)	7
AsuC2I	CCSGG	2 cut(s)	775, 1663
AsuHPI	GGTGA	9 cut(s)	355, 742, 854, 1037, 1598, 1892, 2008, 2015, 2489
AvaI	CYCGRG	1 cut(s)	1133
AvaII	GGWCC	4 cut(s)	454, 807, 1030, 1892
BaeGI	GKGCMC	1 cut(s)	129
BaeI	ACNNNNGTAYC	2 cut(s)	1296, 1329
BalI	TGGCCA	2 cut(s)	1190, 1355
BanI	GGYRCC	1 cut(s)	7
BanII	GRGCYC	3 cut(s)	129, 916, 1440
BauI	CACGAG	1 cut(s)	296
BbsI	GAAGAC	4 cut(s)	1371, 1627, 1694, 1837
Bbv12I	GWGCWC	3 cut(s)	916, 1440, 1731
BbvI	GCAGC	6 cut(s)	46, 138, 668, 1165, 2196, 2420
BccI	CCATC	8 cut(s)	92, 134, 645, 658, 1316, 1362, 1366, 2516
BceAI	ACGGC	1 cut(s)	653
BciT130I	CCWGG	2 cut(s)	1107, 1504
BcnI	CCSGG	2 cut(s)	775, 1663
BcoDI	GTCTC	3 cut(s)	853, 1066, 1559
BfaI	CTAG	3 cut(s)	1763, 2045, 2430
BfmI	CTRYAG	4 cut(s)	16, 60, 1441, 1782
BglII	AGATCT	1 cut(s)	1786
BisI	GCNGC	6 cut(s)	60, 152, 657, 1179, 2185, 2434
BlsI	GCNGC	6 cut(s)	61, 153, 658, 1180, 2186, 2435
BmcAI	AGTACT	1 cut(s)	497
Bme1390I	CCNGG	4 cut(s)	775, 1107, 1504, 1663
Bme18I	GGWCC	4 cut(s)	454, 807, 1030, 1892
BmeRI	GACNNNNNGTC	1 cut(s)	1698
BmeT110I	CYCGRG	1 cut(s)	1133
BmiI	GGNNCC	9 cut(s)	9, 127, 422, 456, 1031, 1032, 1600, 1613, 2130
BmrFI	CCNGG	4 cut(s)	775, 1107, 1504, 1663
BmsI	GCATC	4 cut(s)	706, 1275, 1819, 1921
BoxI	GACNNNNGTC	1 cut(s)	1289
BpiI	GAAGAC	4 cut(s)	1371, 1627, 1694, 1837
BpmI	CTGGAG	1 cut(s)	705
BpuEI	CTTGAG	2 cut(s)	546, 1034
BpuMI	CCSGG	2 cut(s)	775, 1663
BsaI	GGTCTC	1 cut(s)	853
BsaWI	WCCGGW	3 cut(s)	727, 1534, 1601
BsaXI	ACNNNNNCTCC	2 cut(s)	703, 733
Bsc4I	CCNNNNNNNGG	5 cut(s)	308, 566, 816, 1608, 1712
Bse118I	RCCGGY	2 cut(s)	1534, 2016
Bse1I	ACTGG	4 cut(s)	302, 821, 1780, 2027
Bse3DI	GCAATG	1 cut(s)	1572
BseAI	TCCGGA	1 cut(s)	1601
BseBI	CCWGG	2 cut(s)	1107, 1504
BseGI	GGATG	8 cut(s)	669, 1266, 1377, 2031, 2073, 2203, 2273, 2508
BseLI	CCNNNNNNNGG	5 cut(s)	308, 566, 816, 1608, 1712
BseMI	GCAATG	1 cut(s)	1572
BseMII	CTCAG	3 cut(s)	437, 839, 2187
BseNI	ACTGG	4 cut(s)	302, 821, 1780, 2027
BseRI	GAGGAG	2 cut(s)	2031, 2141
BseSI	GKGCMC	1 cut(s)	129
BseXI	GCAGC	6 cut(s)	46, 138, 668, 1165, 2196, 2420
Bsh1236I	CGCG	1 cut(s)	2279
BshNI	GGYRCC	1 cut(s)	7
BshTI	ACCGGT	1 cut(s)	1534
BsiHKAI	GWGCWC	3 cut(s)	916, 1440, 1731
BsiHKCI	CYCGRG	1 cut(s)	1133
BsiSI	CCGG	6 cut(s)	728, 774, 1535, 1602, 1662, 2017
BslFI	GGGAC	4 cut(s)	440, 958, 1043, 1520
BslI	CCNNNNNNNGG	5 cut(s)	308, 566, 816, 1608, 1712
BsmAI	GTCTC	3 cut(s)	853, 1066, 1559
BsmFI	GGGAC	4 cut(s)	440, 958, 1043, 1520
BsmI	GAATGC	1 cut(s)	1255
Bso31I	GGTCTC	1 cut(s)	853
BsoBI	CYCGRG	1 cut(s)	1133
Bsp120I	GGGCCC	1 cut(s)	125
Bsp1286I	GDGCHC	4 cut(s)	129, 916, 1440, 1731
Bsp13I	TCCGGA	1 cut(s)	1601
Bsp19I	CCATGG	3 cut(s)	424, 692, 937
BspACI	CCGC	8 cut(s)	5, 864, 892, 1253, 1713, 2277, 2279, 2452
BspCNI	CTCAG	3 cut(s)	438, 838, 2188
BspEI	TCCGGA	1 cut(s)	1601
BspFNI	CGCG	1 cut(s)	2279
BspLI	GGNNCC	9 cut(s)	9, 127, 422, 456, 1031, 1032, 1600, 1613, 2130
BspMAI	CTGCAG	1 cut(s)	64
BspPI	GGATC	7 cut(s)	704, 719, 839, 1831, 1867, 2076, 2333
BspQI	GCTCTTC	1 cut(s)	1619
BspT107I	GGYRCC	1 cut(s)	7
BspTNI	GGTCTC	1 cut(s)	853
BsrDI	GCAATG	1 cut(s)	1572
BsrFI	RCCGGY	2 cut(s)	1534, 2016
BsrI	ACTGG	4 cut(s)	302, 821, 1780, 2027
BssAI	RCCGGY	2 cut(s)	1534, 2016
BssSI	CACGAG	1 cut(s)	296
BssT1I	CCWWGG	6 cut(s)	82, 424, 692, 937, 1025, 2367
Bst2BI	CACGAG	1 cut(s)	296
Bst2UI	CCWGG	2 cut(s)	1107, 1504
Bst4CI	ACNGT	5 cut(s)	1288, 1402, 1431, 1696, 2164
Bst6I	CTCTTC	4 cut(s)	1619, 1848, 2118, 2249
BstDEI	CTNAG	6 cut(s)	446, 825, 1076, 1120, 2196, 2526
BstDSI	CCRYGG	4 cut(s)	424, 692, 937, 2277
BstF5I	GGATG	8 cut(s)	669, 1266, 1377, 2031, 2073, 2203, 2273, 2508
BstFNI	CGCG	1 cut(s)	2279
BstMAI	GTCTC	3 cut(s)	853, 1066, 1559
BstMWI	GCNNNNNNNGC	7 cut(s)	65, 665, 784, 793, 1187, 1685, 2137
BstNI	CCWGG	2 cut(s)	1107, 1504
BstNSI	RCATGY	2 cut(s)	934, 1513
BstPAI	GACNNNNGTC	1 cut(s)	1289
BstSCI	CCNGG	4 cut(s)	773, 1105, 1502, 1661
BstSFI	CTRYAG	4 cut(s)	16, 60, 1441, 1782
BstSLI	GKGCMC	1 cut(s)	129
BstUI	CGCG	1 cut(s)	2279
BstV1I	GCAGC	6 cut(s)	46, 138, 668, 1165, 2196, 2420
BstV2I	GAAGAC	4 cut(s)	1371, 1627, 1694, 1837
BstX2I	RGATCY	3 cut(s)	1786, 1836, 2081
BstXI	CCANNNNNNTGG	2 cut(s)	101, 222
BstYI	RGATCY	3 cut(s)	1786, 1836, 2081
BtgI	CCRYGG	4 cut(s)	424, 692, 937, 2277
BtsCI	GGATG	8 cut(s)	669, 1266, 1377, 2031, 2073, 2203, 2273, 2508
BtsI	GCAGTG	1 cut(s)	2413
BtsIMutI	CAGTG	5 cut(s)	309, 1773, 1848, 2020, 2413
Cfr10I	RCCGGY	2 cut(s)	1534, 2016
Cfr42I	CCGCGG	1 cut(s)	2280
CspAI	ACCGGT	1 cut(s)	1534
CspCI	CAANNNNNGTGG	2 cut(s)	1784, 1819
DdeI	CTNAG	6 cut(s)	446, 825, 1076, 1120, 2196, 2526
DriI	GACNNNNNGTC	1 cut(s)	1698
EaeI	YGGCCR	4 cut(s)	666, 1188, 1353, 2014
Eam1104I	CTCTTC	4 cut(s)	1619, 1848, 2118, 2249
Eam1105I	GACNNNNNGTC	1 cut(s)	1698
EarI	CTCTTC	4 cut(s)	1619, 1848, 2118, 2249
Ecl136II	GAGCTC	2 cut(s)	914, 1438
Eco130I	CCWWGG	6 cut(s)	82, 424, 692, 937, 1025, 2367
Eco24I	GRGCYC	3 cut(s)	129, 916, 1440
Eco31I	GGTCTC	1 cut(s)	853
Eco32I	GATATC	1 cut(s)	2491
Eco47I	GGWCC	4 cut(s)	454, 807, 1030, 1892
Eco53kI	GAGCTC	2 cut(s)	914, 1438
Eco57I	CTGAAG	3 cut(s)	1365, 1872, 1959
Eco88I	CYCGRG	1 cut(s)	1133
EcoICRI	GAGCTC	2 cut(s)	914, 1438
EcoO109I	RGGNCCY	4 cut(s)	125, 454, 1030, 1742
EcoRII	CCWGG	2 cut(s)	1105, 1502
EcoRV	GATATC	1 cut(s)	2491
EcoT14I	CCWWGG	6 cut(s)	82, 424, 692, 937, 1025, 2367
EcoT38I	GRGCYC	3 cut(s)	129, 916, 1440
ErhI	CCWWGG	6 cut(s)	82, 424, 692, 937, 1025, 2367
FalI	AAGNNNNNCTT	4 cut(s)	990, 1022, 2447, 2479
FaqI	GGGAC	4 cut(s)	440, 958, 1043, 1520
FauI	CCCGC	2 cut(s)	871, 899
FauNDI	CATATG	1 cut(s)	136
FblI	GTMKAC	1 cut(s)	1293
Fnu4HI	GCNGC	6 cut(s)	60, 152, 657, 1179, 2185, 2434
FokI	GGATG	8 cut(s)	676, 1253, 1384, 2038, 2060, 2190, 2260, 2495
FriOI	GRGCYC	3 cut(s)	129, 916, 1440
Fsp4HI	GCNGC	6 cut(s)	60, 152, 657, 1179, 2185, 2434
FspBI	CTAG	3 cut(s)	1763, 2045, 2430
GluI	GCNGC	6 cut(s)	60, 152, 657, 1179, 2185, 2434
GsuI	CTGGAG	1 cut(s)	705
HapII	CCGG	6 cut(s)	728, 774, 1535, 1602, 1662, 2017
HincII	GTYRAC	2 cut(s)	1669, 2167
HindII	GTYRAC	2 cut(s)	1669, 2167
HindIII	AAGCTT	3 cut(s)	356, 2329, 2456
HinfI	GANTC	6 cut(s)	268, 636, 1042, 1376, 1498, 2381
HpaII	CCGG	6 cut(s)	728, 774, 1535, 1602, 1662, 2017
HphI	GGTGA	9 cut(s)	355, 742, 854, 1037, 1598, 1892, 2008, 2015, 2489
Hpy166II	GTNNAC	5 cut(s)	529, 1294, 1479, 1669, 2167
Hpy188I	TCNGA	9 cut(s)	239, 650, 1343, 1384, 1413, 1465, 2065, 2270, 2445
Hpy8I	GTNNAC	5 cut(s)	529, 1294, 1479, 1669, 2167
Hpy99I	CGWCG	1 cut(s)	1622
HpyAV	CCTTC	7 cut(s)	398, 744, 1256, 1513, 1934, 2095, 2117
HpyCH4III	ACNGT	5 cut(s)	1288, 1402, 1431, 1696, 2164
HpyCH4IV	ACGT	1 cut(s)	1752
HpyF10VI	GCNNNNNNNGC	7 cut(s)	65, 665, 784, 793, 1187, 1685, 2137
HpyF3I	CTNAG	6 cut(s)	446, 825, 1076, 1120, 2196, 2526
HpySE526I	ACGT	1 cut(s)	1752
KflI	GGGWCCC	1 cut(s)	1030
Kpn2I	TCCGGA	1 cut(s)	1601
KpnI	GGTACC	1 cut(s)	11
KroI	GCCGGC	1 cut(s)	2016
KroNI	GCCGGC	1 cut(s)	2018
KspI	CCGCGG	1 cut(s)	2280
LguI	GCTCTTC	1 cut(s)	1619
LmnI	GCTCC	8 cut(s)	724, 911, 1435, 1611, 1715, 1726, 1939, 2128
Lsp1109I	GCAGC	6 cut(s)	46, 138, 668, 1165, 2196, 2420
LweI	GCATC	4 cut(s)	706, 1275, 1819, 1921
MaeI	CTAG	3 cut(s)	1763, 2045, 2430
MaeII	ACGT	1 cut(s)	1752
MaeIII	GTNAC	6 cut(s)	130, 382, 514, 743, 1043, 1559
MfeI	CAATTG	1 cut(s)	1215
MflI	RGATCY	3 cut(s)	1786, 1836, 2081
MhlI	GDGCHC	4 cut(s)	129, 916, 1440, 1731
MlsI	TGGCCA	2 cut(s)	1190, 1355
MluCI	AATT	8 cut(s)	192, 319, 949, 1198, 1215, 1222, 1493, 1545
MluNI	TGGCCA	2 cut(s)	1190, 1355
MlyI	GAGTC	3 cut(s)	1051, 1385, 2375
MmeI	TCCRAC	3 cut(s)	1413, 1418, 2272
Mox20I	TGGCCA	2 cut(s)	1190, 1355
MroI	TCCGGA	1 cut(s)	1601
MroNI	GCCGGC	1 cut(s)	2016
MroXI	GAANNNNTTC	2 cut(s)	1389, 1527
MscI	TGGCCA	2 cut(s)	1190, 1355
MseI	TTAA	6 cut(s)	29, 354, 735, 1221, 1655, 1962
MslI	CAYNNNNRTG	4 cut(s)	99, 1514, 1734, 2513
Msp20I	TGGCCA	2 cut(s)	1190, 1355
MspA1I	CMGCKG	2 cut(s)	59, 2279
MspI	CCGG	6 cut(s)	728, 774, 1535, 1602, 1662, 2017
MspR9I	CCNGG	4 cut(s)	775, 1107, 1504, 1663
MunI	CAATTG	1 cut(s)	1215
Mva1269I	GAATGC	1 cut(s)	1255
MvaI	CCWGG	2 cut(s)	1107, 1504
MvnI	CGCG	1 cut(s)	2279
MwoI	GCNNNNNNNGC	7 cut(s)	65, 665, 784, 793, 1187, 1685, 2137
NaeI	GCCGGC	1 cut(s)	2018
NciI	CCSGG	2 cut(s)	775, 1663
NcoI	CCATGG	3 cut(s)	424, 692, 937
NdeI	CATATG	1 cut(s)	136
NgoMIV	GCCGGC	1 cut(s)	2016
NlaIV	GGNNCC	9 cut(s)	9, 127, 422, 456, 1031, 1032, 1600, 1613, 2130
NmuCI	GTSAC	3 cut(s)	514, 1043, 1559
NspI	RCATGY	2 cut(s)	934, 1513
OliI	CACNNNNGTG	1 cut(s)	99
PaeR7I	CTCGAG	1 cut(s)	1133
PasI	CCCWGGG	1 cut(s)	1503
PciI	ACATGT	2 cut(s)	930, 1509
PciSI	GCTCTTC	1 cut(s)	1619
PctI	GAATGC	1 cut(s)	1255
PdiI	GCCGGC	1 cut(s)	2018
PdmI	GAANNNNTTC	2 cut(s)	1389, 1527
PfeI	GAWTC	3 cut(s)	268, 636, 1498
PinAI	ACCGGT	1 cut(s)	1534
PkrI	GCNGC	6 cut(s)	61, 153, 658, 1180, 2186, 2435
PleI	GAGTC	3 cut(s)	1050, 1384, 2375
PpsI	GAGTC	3 cut(s)	1050, 1384, 2375
PpuMI	RGGWCCY	2 cut(s)	454, 1030
PscI	ACATGT	2 cut(s)	930, 1509
PshAI	GACNNNNGTC	1 cut(s)	1289
PshBI	ATTAAT	1 cut(s)	735
Psp124BI	GAGCTC	2 cut(s)	916, 1440
Psp5II	RGGWCCY	2 cut(s)	454, 1030
Psp6I	CCWGG	2 cut(s)	1105, 1502
PspGI	CCWGG	2 cut(s)	1105, 1502
PspN4I	GGNNCC	9 cut(s)	9, 127, 422, 456, 1031, 1032, 1600, 1613, 2130
PspOMI	GGGCCC	1 cut(s)	125
PspPPI	RGGWCCY	2 cut(s)	454, 1030
PspXI	VCTCGAGB	1 cut(s)	1133
PstI	CTGCAG	1 cut(s)	64
PsuI	RGATCY	3 cut(s)	1786, 1836, 2081
PvuII	CAGCTG	1 cut(s)	59
RseI	CAYNNNNRTG	4 cut(s)	99, 1514, 1734, 2513
SacI	GAGCTC	2 cut(s)	916, 1440
SacII	CCGCGG	1 cut(s)	2280
SapI	GCTCTTC	1 cut(s)	1619
SaqAI	TTAA	6 cut(s)	29, 354, 735, 1221, 1655, 1962
SatI	GCNGC	6 cut(s)	60, 152, 657, 1179, 2185, 2434
ScaI	AGTACT	1 cut(s)	497
SchI	GAGTC	3 cut(s)	1051, 1385, 2375
ScrFI	CCNGG	4 cut(s)	775, 1107, 1504, 1663
SduI	GDGCHC	4 cut(s)	129, 916, 1440, 1731
SfaNI	GCATC	4 cut(s)	706, 1275, 1819, 1921
SfcI	CTRYAG	4 cut(s)	16, 60, 1441, 1782
Sfr274I	CTCGAG	1 cut(s)	1133
Sfr303I	CCGCGG	1 cut(s)	2280
SgrBI	CCGCGG	1 cut(s)	2280
SinI	GGWCC	4 cut(s)	454, 807, 1030, 1892
SlaI	CTCGAG	1 cut(s)	1133
SmiMI	CAYNNNNRTG	4 cut(s)	99, 1514, 1734, 2513
SmlI	CTYRAG	3 cut(s)	561, 1049, 1133
SmoI	CTYRAG	3 cut(s)	561, 1049, 1133
Sse9I	AATT	8 cut(s)	192, 319, 949, 1198, 1215, 1222, 1493, 1545
SsiI	CCGC	8 cut(s)	5, 864, 892, 1253, 1713, 2277, 2279, 2452
SspI	AATATT	1 cut(s)	468
SspMI	CTAG	3 cut(s)	1763, 2045, 2430
SstI	GAGCTC	2 cut(s)	916, 1440
StyD4I	CCNGG	4 cut(s)	773, 1105, 1502, 1661
StyI	CCWWGG	6 cut(s)	82, 424, 692, 937, 1025, 2367
TaaI	ACNGT	5 cut(s)	1288, 1402, 1431, 1696, 2164
TaiI	ACGT	1 cut(s)	1755
TaqI	TCGA	4 cut(s)	391, 1134, 1862, 2313
TaqII	GACCGA	3 cut(s)	728, 824, 1547
TasI	AATT	8 cut(s)	192, 319, 949, 1198, 1215, 1222, 1493, 1545
TatI	WGTACW	2 cut(s)	495, 828
TfiI	GAWTC	3 cut(s)	268, 636, 1498
Tru1I	TTAA	6 cut(s)	29, 354, 735, 1221, 1655, 1962
Tru9I	TTAA	6 cut(s)	29, 354, 735, 1221, 1655, 1962
TscAI	CASTG	5 cut(s)	309, 1780, 1855, 2027, 2420
TseFI	GTSAC	3 cut(s)	514, 1043, 1559
TseI	GCWGC	6 cut(s)	59, 151, 656, 1178, 2184, 2433
Tsp45I	GTSAC	3 cut(s)	514, 1043, 1559
TspDTI	ATGAA	8 cut(s)	185, 340, 394, 518, 1532, 2077, 2179, 2340
TspGWI	ACGGA	1 cut(s)	762
TspRI	CASTG	5 cut(s)	309, 1780, 1855, 2027, 2420
VpaK11BI	GGWCC	4 cut(s)	454, 807, 1030, 1892
VspI	ATTAAT	1 cut(s)	735
XceI	RCATGY	2 cut(s)	934, 1513
XhoI	CTCGAG	1 cut(s)	1133
XmiI	GTMKAC	1 cut(s)	1293
XmnI	GAANNNNTTC	2 cut(s)	1389, 1527
XspI	CTAG	3 cut(s)	1763, 2045, 2430
ZrmI	AGTACT	1 cut(s)	497

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.