Rh6CG011300

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0002763
Physical Location & Seq
Forward (+)
1303907 .. 1309735
5829 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG011300.1

Sequence Viewer

Length: 2145 bp
ATGTATGCTACCCTCAGGGTTGGACCATTCATCCAAGCAGAATGGAACCATGGAGGTCTTCCATATTGGCTGAGAGAGGTCCCAGGAATCACTTTCCGATCAGATAATGAACCATACAAGAAACACATGGAGCAATATGTGACCAAGATAATGAAGATGATGAAGGATGAAAAGCTATTTGCTCCACAAGGAGGTCCAATCATCTTAGCTCAGATTGAGAATGAGTACAATCAAATCCAACTTGCATATAGAGAGTTGGGAGATACATATGTGCAGTGGGCAGCAAAGCTGGCAGTTGGGCAGAATATTGGAGTGCCATGGATCATGTGCAAGCAAAAGGATGCTCCTGATCCAGTGATTAATACTTGCAACGGAAGGCATTGTGGAGATACTTTTTCAGGACCTAACAAACCCAACAAGCCTGCTCTATGGACTGAGAATTGGACAGCTCAGTACCGAGTATTTGGTGACCCTCCTTCACAAAGAGCAGCAGAAGACATTGCATTTTCAGTTGCTCGGTTTTTCTCCAAGGGTGGGGCTCTAACCAACTACTATATGTACCATGGTGGAACGAATTTTGGAAGAACAAGTGCTGTTTTCACAACAACTCGTTACTACGATGAGGCTCCTCTCGATGAATATGCTTTGCAAAGGGACCCGAAATGGAGTCACCTCAAGGACTTGCACAAGGCTATAAATCTATGCAAGAAAACTTTGCTTACTGGAACTCCCGGAGACCAAAGGTTGGGGGAAGAAACTGAGGTTCGTTTCTATGAGAAGCCAGGGACAGAGTGTGCTGCTTTCATAGCAAATAATCACTCGACCATGGAAGCAACCGTTAATTGGAGGGGACAGAAGTTTCTCCTGCCACCAGCTTCCATTAGCATCCTCCCCGACTGCAAGACTGTGGTCTTCAATACTCAACAAATTGTATCCCAACATAATTCAAGGAACTTTGTTACAGCTAGTAAACAAATGAACTGGGAAATGTCTCCCGAACCCATTGCAACTGTCGCACAAATCCCATCCAATAGTAGGGAAATGCAGGAGCTCTATGGCTTGCTAAAAGATACCACAGACTATGCATGGTACACAACTAGCATTGACCTAAGTCCATATGACTTGCCGATGAAGGAAACTATCAGGCCAGTTGTACGAATTCCTAGTCTTGGTCATGCAATGGCTGTATTTGTCAATGATGAATATATTGGATCGCAACACGGTAGCCACGCAGAGAAAGGTTTCGTGTTCGAGGCACCTGCTAAAATGAAGCTAGGGGCGAACCATATCTCTCTGCTTTGCATGGTAGTGGGACTCCCAGATAGTGGAGCGTACATGGAGCACAGGTATGCAGGGCCTCGTTCTGTAACAATCCTAGGATTGAACACCGGAACAATCGACCTGTCCGCAAATGGCTGGGGACATAGGGCTGGCCTGAATGGTGAAAAGCTTGAAGTGTACACTGAGAAGGGAGCTCAGAAAGTGAAATGGACTAAGGCAACAGGTAAAGGAAGGGCTCTCACATGGTACAAGACAAGTTTTGATGCTCCTTCAGGAAGAGACCCTTGTGCTATTCGAATGACCGGTATGGGCAAGGGAATGGTATGGGTGAATGGTATAAGCATCGGCCGTCATTGGATGTCCTTCCTCTCTCCTCTTGGACAACCTACTCAAACCGAGTACCACATCCCAAGAGCCTTCATTAAGCCTAAGAATTTCCTTGTTGTATTGGAGGAGGAGCCAGCAGACCCAAAAGACCTTGAGATCGTAACAGTCAACAGAGACACCATCTGCAGCCTTATTAAACAGGACTATCCGGGACATGTTAAGTACTGGGATAGAAAAGGCGGCAAGCTCACAACAGCTCCTGGAAAAAGTCTCCAACCAGCAGCTCTCCTCAACTGTCCAAACTACAAAAAGATCAGTGCTATTGAATTCGCGGGCTATGGCAATCCTGACGGCTTCTGTGGAGCCTTTAAATTGGGACAATGCAATGCTCCTGAAGCCAAGAAGGTCGTCGAAGATCATTGCTTGGGAAAGATTTCATGTACAGTCCCAAATGACCCCAGCCTCTTCTTCAAGTCTGGTGATCCTTGCCCTGGTATCAAGAAGACACTAGCTATCCAAGCAGCGTGCAGCTTTTAA

Protein Analysis

714

Amino Acids

79.5

Weight (kDa)

8.63

Isoelectric Point (pI)

34.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 1 - 231 1.6e-76 Glycosyl hydrolases family 35
GHD PF17834 255 - 310 3.3e-20 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 507 - 571 1.2e-22 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 633 - 712 2.7e-17 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1267
Acc36I ACCTGC 1 cut(s) 1267
AccB1I GGYRCC 1 cut(s) 1255
AccB7I CCANNNNNTGG 2 cut(s) 745, 1325
AccII CGCG 1 cut(s) 1940
AciI CCGC 3 cut(s) 1407, 1848, 1940
AclWI GGATC 4 cut(s) 329, 344, 1219, 2084
AcoI YGGCCR 1 cut(s) 1627
AcsI RAATTY 4 cut(s) 574, 1158, 1714, 1934
AcuI CTGAAG 2 cut(s) 1536, 2022
AfiI CCNNNNNNNGG 8 cut(s) 191, 534, 745, 843, 1035, 1169, 1325, 2099
AflIII ACRYGT 1 cut(s) 1822
AgeI ACCGGT 1 cut(s) 1583
AgsI TTSAA 6 cut(s) 916, 948, 1384, 1454, 1934, 2080
AjnI CCWGG 4 cut(s) 82, 781, 1867, 2098
Alw21I GWGCWC 3 cut(s) 1053, 1344, 1477
Alw26I GTCTC 5 cut(s) 729, 996, 1554, 1776, 1883
AlwI GGATC 4 cut(s) 329, 344, 1219, 2084
AlwNI CAGNNNCTG 1 cut(s) 1868
AoxI GGCC 4 cut(s) 1145, 1355, 1432, 1627
ApeKI GCWGC 7 cut(s) 281, 488, 797, 1794, 1889, 2129, 2136
ApoI RAATTY 4 cut(s) 574, 1158, 1714, 1934
ArsI GACNNNNNNTTYG 2 cut(s) 1150, 1182
AseI ATTAAT 1 cut(s) 360
AsiGI ACCGGT 1 cut(s) 1583
Asp700I GAANNNNTTC 2 cut(s) 1241, 2041
AspA2I CCTAGG 1 cut(s) 1375
AspS9I GGNCC 6 cut(s) 23, 79, 194, 401, 655, 1355
AsuC2I CCSGG 2 cut(s) 732, 1818
AsuHPI GGTGA 5 cut(s) 479, 662, 1454, 1621, 2099
AsuII TTCGAA 1 cut(s) 1576
AvaII GGWCC 5 cut(s) 23, 79, 194, 401, 655
AvrII CCTAGG 1 cut(s) 1375
AxyI CCTNAGG 1 cut(s) 14
BaeI ACNNNNGTAYC 2 cut(s) 255, 288
BanI GGYRCC 1 cut(s) 1255
BanII GRGCYC 4 cut(s) 541, 1053, 1477, 1519
BbsI GAAGAC 4 cut(s) 50, 501, 904, 2117
Bbv12I GWGCWC 3 cut(s) 1053, 1344, 1477
BbvI GCAGC 6 cut(s) 293, 500, 784, 1806, 1901, 2141
BccI CCATC 2 cut(s) 1033, 1796
BceAI ACGGC 2 cut(s) 1614, 1975
BcgI CGANNNNNNTGC 6 cut(s) 623, 657, 986, 1020, 1241, 1275
BciT130I CCWGG 4 cut(s) 84, 783, 1869, 2100
BciVI GTATCC 1 cut(s) 943
BcnI CCSGG 2 cut(s) 732, 1818
BcoDI GTCTC 5 cut(s) 729, 996, 1554, 1776, 1883
BfaI CTAG 6 cut(s) 966, 1098, 1164, 1274, 1376, 2117
BfmI CTRYAG 1 cut(s) 1792
BfuAI ACCTGC 1 cut(s) 1267
BfuI GTATCC 1 cut(s) 943
BisI GCNGC 8 cut(s) 282, 489, 798, 1795, 1849, 1890, 2130, 2137
BlnI CCTAGG 1 cut(s) 1375
BlsI GCNGC 8 cut(s) 283, 490, 799, 1796, 1850, 1891, 2131, 2138
BmcAI AGTACT 1 cut(s) 1832
Bme1390I CCNGG 6 cut(s) 84, 732, 783, 1818, 1869, 2100
Bme18I GGWCC 5 cut(s) 23, 79, 194, 401, 655
BmgT120I GGNCC 6 cut(s) 23, 79, 194, 401, 655, 1355
BmiI GGNNCC 8 cut(s) 47, 81, 627, 656, 657, 1257, 1740, 1972
BmrFI CCNGG 6 cut(s) 84, 732, 783, 1818, 1869, 2100
BmrI ACTGGG 2 cut(s) 991, 1843
BmsI GCATC 4 cut(s) 331, 894, 1534, 1632
BmuI ACTGGG 2 cut(s) 991, 1843
BoxI GACNNNNGTC 2 cut(s) 908, 1110
BpiI GAAGAC 4 cut(s) 50, 501, 904, 2117
Bpu14I TTCGAA 1 cut(s) 1576
BpuEI CTTGAG 2 cut(s) 659, 1781
BpuMI CCSGG 2 cut(s) 732, 1818
BsaI GGTCTC 2 cut(s) 729, 1554
BsaJI CCNNGG 9 cut(s) 49, 82, 317, 528, 562, 782, 825, 1375, 2098
BsaWI WCCGGW 2 cut(s) 1388, 1583
BsaXI ACNNNNNCTCC 6 cut(s) 712, 742, 1331, 1361, 1849, 1879
Bsc4I CCNNNNNNNGG 8 cut(s) 191, 534, 745, 843, 1035, 1169, 1325, 2099
Bse118I RCCGGY 1 cut(s) 1583
Bse1I ACTGG 5 cut(s) 353, 727, 986, 1148, 1838
Bse21I CCTNAGG 1 cut(s) 14
Bse3DI GCAATG 5 cut(s) 498, 1002, 1185, 1999, 2026
BseBI CCWGG 4 cut(s) 84, 783, 1869, 2100
BseDI CCNNGG 9 cut(s) 49, 82, 317, 528, 562, 782, 825, 1375, 2098
BseGI GGATG 7 cut(s) 30, 172, 346, 885, 1025, 1644, 1686
BseLI CCNNNNNNNGG 8 cut(s) 191, 534, 745, 843, 1035, 1169, 1325, 2099
BseMI GCAATG 5 cut(s) 498, 1002, 1185, 1999, 2026
BseMII CTCAG 8 cut(s) 28, 62, 224, 426, 464, 750, 1455, 1490
BseNI ACTGG 5 cut(s) 353, 727, 986, 1148, 1838
BseRI GAGGAG 5 cut(s) 618, 1644, 1748, 1751, 1886
BseX3I CGGCCG 1 cut(s) 1627
BseXI GCAGC 6 cut(s) 293, 500, 784, 1806, 1901, 2141
BseYI CCCAGC 2 cut(s) 1416, 2066
BsgI GTGCAG 1 cut(s) 293
Bsh1236I CGCG 1 cut(s) 1940
Bsh1285I CGRYCG 1 cut(s) 1630
BshFI GGCC 4 cut(s) 1147, 1357, 1434, 1629
BshNI GGYRCC 1 cut(s) 1255
BshTI ACCGGT 1 cut(s) 1583
BsiEI CGRYCG 1 cut(s) 1630
BsiHKAI GWGCWC 3 cut(s) 1053, 1344, 1477
BsiSI CCGG 4 cut(s) 732, 1389, 1584, 1817
BslFI GGGAC 9 cut(s) 65, 668, 799, 864, 1326, 1434, 1833, 1998, 2039
BslI CCNNNNNNNGG 8 cut(s) 191, 534, 745, 843, 1035, 1169, 1325, 2099
BsmAI GTCTC 5 cut(s) 729, 996, 1554, 1776, 1883
BsmFI GGGAC 9 cut(s) 65, 668, 799, 864, 1326, 1434, 1833, 1998, 2039
BsnI GGCC 4 cut(s) 1147, 1357, 1434, 1629
Bso31I GGTCTC 2 cut(s) 729, 1554
Bsp119I TTCGAA 1 cut(s) 1576
Bsp1286I GDGCHC 5 cut(s) 541, 1053, 1344, 1477, 1519
Bsp1407I TGTACA 2 cut(s) 1458, 2048
Bsp143I GATC 8 cut(s) 98, 321, 349, 1211, 1764, 1920, 2023, 2089
Bsp19I CCATGG 4 cut(s) 49, 317, 562, 825
BspACI CCGC 3 cut(s) 1407, 1848, 1940
BspANI GGCC 4 cut(s) 1147, 1357, 1434, 1629
BspCNI CTCAG 8 cut(s) 27, 63, 223, 427, 463, 751, 1456, 1489
BspFNI CGCG 1 cut(s) 1940
BspLI GGNNCC 8 cut(s) 47, 81, 627, 656, 657, 1257, 1740, 1972
BspMAI CTGCAG 1 cut(s) 1796
BspMI ACCTGC 1 cut(s) 1267
BspPI GGATC 4 cut(s) 329, 344, 1219, 2084
BspT104I TTCGAA 1 cut(s) 1576
BspT107I GGYRCC 1 cut(s) 1255
BspTNI GGTCTC 2 cut(s) 729, 1554
BsrDI GCAATG 5 cut(s) 498, 1002, 1185, 1999, 2026
BsrFI RCCGGY 1 cut(s) 1583
BsrGI TGTACA 2 cut(s) 1458, 2048
BsrI ACTGG 5 cut(s) 353, 727, 986, 1148, 1838
BssAI RCCGGY 1 cut(s) 1583
BssECI CCNNGG 9 cut(s) 49, 82, 317, 528, 562, 782, 825, 1375, 2098
BssMI GATC 8 cut(s) 98, 321, 349, 1211, 1764, 1920, 2023, 2089
BssT1I CCWWGG 6 cut(s) 49, 317, 528, 562, 825, 1375
Bst2UI CCWGG 4 cut(s) 84, 783, 1869, 2100
Bst4CI ACNGT 7 cut(s) 838, 907, 1012, 1223, 1774, 1904, 2053
Bst6I CTCTTC 2 cut(s) 1552, 2078
BstAUI TGTACA 2 cut(s) 1458, 2048
BstBI TTCGAA 1 cut(s) 1576
BstC8I GCNNGC 9 cut(s) 291, 332, 423, 1061, 1432, 1743, 1853, 1942, 2134
BstDSI CCRYGG 4 cut(s) 49, 317, 562, 825
BstEII GGTNACC 1 cut(s) 467
BstF5I GGATG 7 cut(s) 30, 172, 346, 885, 1025, 1644, 1686
BstFNI CGCG 1 cut(s) 1940
BstKTI GATC 8 cut(s) 101, 324, 352, 1214, 1767, 1923, 2026, 2092
BstMAI GTCTC 5 cut(s) 729, 996, 1554, 1776, 1883
BstMBI GATC 8 cut(s) 98, 321, 349, 1211, 1764, 1920, 2023, 2089
BstMCI CGRYCG 1 cut(s) 1630
BstMWI GCNNNNNNNGC 5 cut(s) 290, 806, 1013, 2003, 2126
BstNI CCWGG 4 cut(s) 84, 783, 1869, 2100
BstNSI RCATGY 1 cut(s) 1826
BstPAI GACNNNNGTC 2 cut(s) 908, 1110
BstPI GGTNACC 1 cut(s) 467
BstSCI CCNGG 6 cut(s) 82, 730, 781, 1816, 1867, 2098
BstSFI CTRYAG 1 cut(s) 1792
BstUI CGCG 1 cut(s) 1940
BstV1I GCAGC 6 cut(s) 293, 500, 784, 1806, 1901, 2141
BstV2I GAAGAC 4 cut(s) 50, 501, 904, 2117
BstZI CGGCCG 1 cut(s) 1627
Bsu36I CCTNAGG 1 cut(s) 14
BsuI GTATCC 1 cut(s) 943
BsuRI GGCC 4 cut(s) 1147, 1357, 1434, 1629
BtgI CCRYGG 4 cut(s) 49, 317, 562, 825
BtsCI GGATG 7 cut(s) 30, 172, 346, 885, 1025, 1644, 1686
BtsI GCAGTG 1 cut(s) 281
BtsIMutI CAGTG 4 cut(s) 281, 360, 1461, 1930
BveI ACCTGC 1 cut(s) 1267
Cac8I GCNNGC 9 cut(s) 291, 332, 423, 1061, 1432, 1743, 1853, 1942, 2134
CaiI CAGNNNCTG 1 cut(s) 1868
Cfr10I RCCGGY 1 cut(s) 1583
Cfr13I GGNCC 6 cut(s) 23, 79, 194, 401, 655, 1355
CspAI ACCGGT 1 cut(s) 1583
DpnI GATC 8 cut(s) 100, 323, 351, 1213, 1766, 1922, 2025, 2091
DpnII GATC 8 cut(s) 98, 321, 349, 1211, 1764, 1920, 2023, 2089
DraI TTTAAA 1 cut(s) 1978
EaeI YGGCCR 1 cut(s) 1627
EagI CGGCCG 1 cut(s) 1627
Eam1104I CTCTTC 2 cut(s) 1552, 2078
EarI CTCTTC 2 cut(s) 1552, 2078
Ecl136II GAGCTC 2 cut(s) 1051, 1475
EclXI CGGCCG 1 cut(s) 1627
Eco130I CCWWGG 6 cut(s) 49, 317, 528, 562, 825, 1375
Eco24I GRGCYC 4 cut(s) 541, 1053, 1477, 1519
Eco31I GGTCTC 2 cut(s) 729, 1554
Eco47I GGWCC 5 cut(s) 23, 79, 194, 401, 655
Eco52I CGGCCG 1 cut(s) 1627
Eco53kI GAGCTC 2 cut(s) 1051, 1475
Eco57I CTGAAG 2 cut(s) 1536, 2022
Eco81I CCTNAGG 1 cut(s) 14
Eco91I GGTNACC 1 cut(s) 467
EcoICRI GAGCTC 2 cut(s) 1051, 1475
EcoO109I RGGNCCY 4 cut(s) 79, 401, 655, 1355
EcoO65I GGTNACC 1 cut(s) 467
EcoRI GAATTC 2 cut(s) 1158, 1934
EcoRII CCWGG 4 cut(s) 82, 781, 1867, 2098
EcoT14I CCWWGG 6 cut(s) 49, 317, 528, 562, 825, 1375
EcoT22I ATGCAT 1 cut(s) 1087
EcoT38I GRGCYC 4 cut(s) 541, 1053, 1477, 1519
ErhI CCWWGG 6 cut(s) 49, 317, 528, 562, 825, 1375
FalI AAGNNNNNCTT 2 cut(s) 1549, 1581
FaqI GGGAC 9 cut(s) 65, 668, 799, 864, 1326, 1434, 1833, 1998, 2039
FauI CCCGC 1 cut(s) 1933
FauNDI CATATG 2 cut(s) 268, 1117
Fnu4HI GCNGC 8 cut(s) 282, 489, 798, 1795, 1849, 1890, 2130, 2137
FokI GGATG 7 cut(s) 17, 179, 353, 872, 1012, 1651, 1673
FriOI GRGCYC 4 cut(s) 541, 1053, 1477, 1519
Fsp4HI GCNGC 8 cut(s) 282, 489, 798, 1795, 1849, 1890, 2130, 2137
FspBI CTAG 6 cut(s) 966, 1098, 1164, 1274, 1376, 2117
GluI GCNGC 8 cut(s) 282, 489, 798, 1795, 1849, 1890, 2130, 2137
GsaI CCCAGC 2 cut(s) 1420, 2070
HaeIII GGCC 4 cut(s) 1147, 1357, 1434, 1629
HapII CCGG 4 cut(s) 732, 1389, 1584, 1817
HincII GTYRAC 1 cut(s) 1777
HindII GTYRAC 1 cut(s) 1777
HindIII AAGCTT 1 cut(s) 1448
HinfI GANTC 3 cut(s) 87, 667, 1314
HpaII CCGG 4 cut(s) 732, 1389, 1584, 1817
HphI GGTGA 5 cut(s) 479, 662, 1454, 1621, 2099
Hpy166II GTNNAC 5 cut(s) 971, 1092, 1459, 1461, 1777
Hpy188I TCNGA 4 cut(s) 98, 103, 213, 1479
Hpy188III TCNNGA 8 cut(s) 347, 399, 632, 995, 1554, 1955, 2000, 2107
Hpy8I GTNNAC 5 cut(s) 971, 1092, 1459, 1461, 1777
Hpy99I CGWCG 1 cut(s) 2021
HpyCH4III ACNGT 7 cut(s) 838, 907, 1012, 1223, 1774, 1904, 2053
HpyF10VI GCNNNNNNNGC 5 cut(s) 290, 806, 1013, 2003, 2126
KflI GGGWCCC 1 cut(s) 655
Kzo9I GATC 8 cut(s) 98, 321, 349, 1211, 1764, 1920, 2023, 2089
Lsp1109I GCAGC 6 cut(s) 293, 500, 784, 1806, 1901, 2141
LweI GCATC 4 cut(s) 331, 894, 1534, 1632
MaeI CTAG 6 cut(s) 966, 1098, 1164, 1274, 1376, 2117
MaeIII GTNAC 7 cut(s) 139, 467, 611, 668, 958, 1366, 1768
MalI GATC 8 cut(s) 100, 323, 351, 1213, 1766, 1922, 2025, 2091
MboI GATC 8 cut(s) 98, 321, 349, 1211, 1764, 1920, 2023, 2089
MhlI GDGCHC 5 cut(s) 541, 1053, 1344, 1477, 1519
MluCI AATT 9 cut(s) 439, 574, 841, 927, 943, 1158, 1714, 1934, 1979
MlyI GAGTC 2 cut(s) 676, 1308
MmeI TCCRAC 2 cut(s) 262, 1906
Mph1103I ATGCAT 1 cut(s) 1087
MroXI GAANNNNTTC 2 cut(s) 1241, 2041
MseI TTAA 7 cut(s) 360, 840, 1704, 1803, 1827, 1977, 2143
MslI CAYNNNNRTG 2 cut(s) 1307, 1347
MspI CCGG 4 cut(s) 732, 1389, 1584, 1817
MspR9I CCNGG 6 cut(s) 84, 732, 783, 1818, 1869, 2100
MvaI CCWGG 4 cut(s) 84, 783, 1869, 2100
MvnI CGCG 1 cut(s) 1940
MwoI GCNNNNNNNGC 5 cut(s) 290, 806, 1013, 2003, 2126
NciI CCSGG 2 cut(s) 732, 1818
NcoI CCATGG 4 cut(s) 49, 317, 562, 825
NdeI CATATG 2 cut(s) 268, 1117
NdeII GATC 8 cut(s) 98, 321, 349, 1211, 1764, 1920, 2023, 2089
NlaIV GGNNCC 8 cut(s) 47, 81, 627, 656, 657, 1257, 1740, 1972
NmuCI GTSAC 3 cut(s) 139, 467, 668
NsiI ATGCAT 1 cut(s) 1087
NspI RCATGY 1 cut(s) 1826
NspV TTCGAA 1 cut(s) 1576
PaqCI CACCTGC 1 cut(s) 1267
PciI ACATGT 1 cut(s) 1822
PdmI GAANNNNTTC 2 cut(s) 1241, 2041
PfeI GAWTC 1 cut(s) 87
PflMI CCANNNNNTGG 2 cut(s) 745, 1325
PfoI TCCNGGA 3 cut(s) 730, 1816, 1867
PinAI ACCGGT 1 cut(s) 1583
PkrI GCNGC 8 cut(s) 283, 490, 799, 1796, 1850, 1891, 2131, 2138
PleI GAGTC 2 cut(s) 675, 1308
PpsI GAGTC 2 cut(s) 675, 1308
PpuMI RGGWCCY 3 cut(s) 79, 401, 655
PscI ACATGT 1 cut(s) 1822
PshAI GACNNNNGTC 2 cut(s) 908, 1110
PshBI ATTAAT 1 cut(s) 360
Psp124BI GAGCTC 2 cut(s) 1053, 1477
Psp5II RGGWCCY 3 cut(s) 79, 401, 655
Psp6I CCWGG 4 cut(s) 82, 781, 1867, 2098
PspEI GGTNACC 1 cut(s) 467
PspFI CCCAGC 2 cut(s) 1416, 2066
PspGI CCWGG 4 cut(s) 82, 781, 1867, 2098
PspN4I GGNNCC 8 cut(s) 47, 81, 627, 656, 657, 1257, 1740, 1972
PspPI GGNCC 6 cut(s) 23, 79, 194, 401, 655, 1355
PspPPI RGGWCCY 3 cut(s) 79, 401, 655
PstI CTGCAG 1 cut(s) 1796
PstNI CAGNNNCTG 1 cut(s) 1868
RseI CAYNNNNRTG 2 cut(s) 1307, 1347
SacI GAGCTC 2 cut(s) 1053, 1477
SaqAI TTAA 7 cut(s) 360, 840, 1704, 1803, 1827, 1977, 2143
SatI GCNGC 8 cut(s) 282, 489, 798, 1795, 1849, 1890, 2130, 2137
Sau3AI GATC 8 cut(s) 98, 321, 349, 1211, 1764, 1920, 2023, 2089
Sau96I GGNCC 6 cut(s) 23, 79, 194, 401, 655, 1355
ScaI AGTACT 1 cut(s) 1832
SchI GAGTC 2 cut(s) 676, 1308
ScrFI CCNGG 6 cut(s) 84, 732, 783, 1818, 1869, 2100
SduI GDGCHC 5 cut(s) 541, 1053, 1344, 1477, 1519
SfaNI GCATC 4 cut(s) 331, 894, 1534, 1632
SfcI CTRYAG 1 cut(s) 1792
SfuI TTCGAA 1 cut(s) 1576
SinI GGWCC 5 cut(s) 23, 79, 194, 401, 655
SmiMI CAYNNNNRTG 2 cut(s) 1307, 1347
SmlI CTYRAG 2 cut(s) 674, 1760
SmoI CTYRAG 2 cut(s) 674, 1760
Sse9I AATT 9 cut(s) 439, 574, 841, 927, 943, 1158, 1714, 1934, 1979
SsiI CCGC 3 cut(s) 1407, 1848, 1940
SspI AATATT 1 cut(s) 307
SspMI CTAG 6 cut(s) 966, 1098, 1164, 1274, 1376, 2117
SstI GAGCTC 2 cut(s) 1053, 1477
StyD4I CCNGG 6 cut(s) 82, 730, 781, 1816, 1867, 2098
StyI CCWWGG 6 cut(s) 49, 317, 528, 562, 825, 1375
TaaI ACNGT 7 cut(s) 838, 907, 1012, 1223, 1774, 1904, 2053
TaqI TCGA 6 cut(s) 633, 821, 1251, 1398, 1576, 2019
TasI AATT 9 cut(s) 439, 574, 841, 927, 943, 1158, 1714, 1934, 1979
TatI WGTACW 4 cut(s) 225, 1458, 1830, 2048
TauI GCSGC 1 cut(s) 1851
TfiI GAWTC 1 cut(s) 87
Tru1I TTAA 7 cut(s) 360, 840, 1704, 1803, 1827, 1977, 2143
Tru9I TTAA 7 cut(s) 360, 840, 1704, 1803, 1827, 1977, 2143
TscAI CASTG 4 cut(s) 281, 360, 1468, 1930
TseFI GTSAC 3 cut(s) 139, 467, 668
TseI GCWGC 7 cut(s) 281, 488, 797, 1794, 1889, 2129, 2136
Tsp45I GTSAC 3 cut(s) 139, 467, 668
TspGWI ACGGA 1 cut(s) 387
TspRI CASTG 4 cut(s) 281, 360, 1468, 1930
Van91I CCANNNNNTGG 2 cut(s) 745, 1325
VpaK11BI GGWCC 5 cut(s) 23, 79, 194, 401, 655
VspI ATTAAT 1 cut(s) 360
XapI RAATTY 4 cut(s) 574, 1158, 1714, 1934
XceI RCATGY 1 cut(s) 1826
XmaJI CCTAGG 1 cut(s) 1375
XmnI GAANNNNTTC 2 cut(s) 1241, 2041
XspI CTAG 6 cut(s) 966, 1098, 1164, 1274, 1376, 2117
ZrmI AGTACT 1 cut(s) 1832
Zsp2I ATGCAT 1 cut(s) 1087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.