MD05G1121000.v1.1

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002763
Physical Location & Seq
Reverse (-)
24366756 .. 24371567
4812 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1121000.v1.1.491

Sequence Viewer

Length: 2592 bp
ATGGTGGTGTCAAGGACTTTTCTGTGGATTTCTTTTGTTCTTTTGGTGGTCTCGGACAGTCTAGTTTATGCCAACGTTAAGAAGCCGGCTGCTGCTGCAGATGGGGTAGCATTGGGGGACTTGGTGGAAGGGGAAGATCCAGTTGTTGAAAAGAGAGACAAGAAGAAACTTGAAGTCGGGGAGGTGACATATGATGGCAGGTCCATCATGATCAACGGAAAACGCGAGATTTTCTTTTCTGGTTCAATTCATTATCCACGGAGCACAGTGGATATGTGGCCTGATCTTCTCAGGAAGGCAAAACAGGGAGGTTTGAATGTGATTCAAACTTATGTCTTTTGGAACCTTCATGAGCCTGTGAAAGGCAAGTGGAATTTCGAGGGAAACGCCGATTTGGTGAAGTTCATCAGGCTGATTCAGGAGAACAATATGTATGTCACCCTCAGGGTAGGGCCATTCATCCAAGCCGAATGGAACCACGGAGGGCTTCCATATTGGCTGAGGGAGGAACCGAACATAGTTTTCCGTTCGGATAATCCAACATACAAGCAACACATGGAGGCATTCGTGGCTAAGATAATGAAGATGATGAAAGATGAGAAGCTATTTGCTCCACAGGGAGGTCCAATCATCTTAGCTCAGGTTGAAAATGAGTACACTCATGTCCAGCGTGCATATAGAGAGTTGGGGGACAGTTATGTGCAGTGGGCAGCAAAAATGGCACTTGCGCAGAACGTCGGTGTGCCATGGATCATGTGCAAACAGAAGGATGCTCCTGATCCAGTCATTAATACTTGCAATGGAAGGCACTGTGGAGACACTTTTCCAGGCCCTAATAAGCCTCACAAGCCTTCTCTATGGACTGAGAACTGGACTGCTCAGTACAGAGTATTTGGTGATCCTCCTTCACAAAGATCGGCAGAAGACATTGCGTTTTCAGTTGCTCGATTTTTCTCCAAGGATGGAACCCTAACCAACTACTATATGTACCATGGTGGAACAAACTTTGGAAGAACAAGTTCTATTTTCACAACAACTCGATACTATGATGAAGCACCTCTTGATGAATATGGTTTGCAAAGGGAGCCGAAATGGGGTCACCTCAAGGACTTGCACAAGGCTTTGAATCTATGCAAGAAGTCTTTGTTAGCAGGAGCTCCTGGAGTCCTCAAGTTGGGCAAAGAAACCGAGGCAAGTATCGCTCGTTTCTATGAGAAGCCTGGAACAGACATATGTGTTGCTTTCCTGGCAAACAACCACTCAAAACTCGAAACAAGTGTCACTTGGCGTGGGCAGGATTATTTCCTGCCACCACATTCTATTAGCATCCTCCCAGACTGCAAGACCGTGGTCTTTAATTCTCAGCAGATTGTGTCACAGCATAATACGAGGAACTTTGTTCGATCAAAGGCTGCTAAAAAACTCAACTGGGAAATGTCTCCAGAACCAATTCCAACCGTTGTGCAGGTACCAGTGAATCAAAAGAAACCACAGGAACTTTACCACTTGCTCAAAGATGCCACAGACTATGGATGGTTTACCACTAGCCTTGACCTAGGTCCATATGACTTGCCGATGAAGGATTCTATTCGCCCGGTTATCCGAATCCCAAGTCTTGGTCACGCAATGGGCGTATTCGTCAATGGTGAATATATCGGAACCGAACACGGAAGCCACGCGGAGAAGGGATTTGTCTTCGAGAAACCTGTCACATTCAAGCAAGGGCTCAACTACATCTCATTGCTTTGCATGACAGTGGGACTCCCAGATAGTGGAGCATACATGGAGCACAGATACGCCGGACCCCGAACCATAACAGTCCTGGGATTGAACACAGGAACAATTGATCTCTCACAAAATGGCTGGGGACATAGGGTTGGCTTGAATGGCGAAAACTTACAAGTGTTCACTGAGAAGGGAGCGCAGAAAGTCCAATGGACTAAAGCTGCAGGCAAAGGAAGGGCTCTCACATGGTACAAGACATATTTTGATGCTCCTGAAGGGAAAGACCCCGTTGCTATCCGGATGACTGGAATGGGAAAGGGAATGGTTTGGGTGAATGGTTTAAGCATCGGGCGGCACTGGATGTCCTTCCTCTCTCCGCTTGGACAACCTACTCAAGCCGAGTACCATATTCCTAGATCTTTCATCAAGCCGGGCAAAAATTTCCTTGTTGTATTGGAGGAACAGGATGCTGACCCTAAAGACATTGAGATCCTAACGGTCAACAGAGACACAATCTGTTCCATGAGTTCACAGATGCATCCTCCACACGTTAATGCATTCGAAAGAAAGGGAGGCAAGTTCCAAATTGTCGCAGGCATCTCTGCCGCAACTGCTCAACTCAAGTGTCCAAACTACAAAAAGATTGTTGCTGTGGAGTTCGCAAGCTTCGGTACTCCTGAAGGCGTATGTGGAACTTACGCCATTGGCAGCTGTGATGCTCCGGGAACCAAAAAGATCGTTGAAGAGAAGTGCTTAGGAAAGACTGCATGTACAGTCCCCAGTGACCCCGGGCTTTTCTTCAAAGTTGATCCTTGCCCAACTGTCAAGATCAAGAGACTTGCGATCCAAGTATTGTGTTCCCACTAA

Protein Analysis

864

Amino Acids

96.52

Weight (kDa)

8.87

Isoelectric Point (pI)

35.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 68 - 374 9.9e-117 Glycosyl hydrolases family 35
Glyco_hydro_42 PF02449 91 - 226 1e-06 Beta-galactosidase
GHD PF17834 403 - 457 1.7e-18 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 656 - 722 9.1e-25 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 782 - 861 1.7e-14 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 729
Acc36I ACCTGC 2 cut(s) 189, 1456
Acc65I GGTACC 1 cut(s) 1468
AccB1I GGYRCC 1 cut(s) 1468
AccB7I CCANNNNNTGG 2 cut(s) 1616, 1772
AccII CGCG 2 cut(s) 225, 1679
AccIII TCCGGA 1 cut(s) 2022
AciI CCGC 4 cut(s) 1679, 2077, 2102, 2331
AclI AACGTT 1 cut(s) 75
AclWI GGATC 7 cut(s) 131, 758, 773, 893, 2209, 2528, 2563
AcsI RAATTY 2 cut(s) 373, 2164
AcuI CTGAAG 2 cut(s) 2019, 2424
AfaI GTAC 8 cut(s) 656, 884, 989, 1470, 1976, 2129, 2398, 2497
AfiI CCNNNNNNNGG 6 cut(s) 362, 620, 1094, 1174, 1616, 1772
AflIII ACRYGT 1 cut(s) 2272
AjnI CCWGG 5 cut(s) 826, 1159, 1219, 1245, 1821
AluBI AGCT 6 cut(s) 604, 638, 1157, 1946, 2391, 2436
AluI AGCT 6 cut(s) 604, 638, 1157, 1946, 2391, 2436
Alw21I GWGCWC 3 cut(s) 266, 1159, 1791
Alw26I GTCTC 6 cut(s) 55, 150, 810, 1443, 2226, 2554
AlwI GGATC 7 cut(s) 131, 758, 773, 893, 2209, 2528, 2563
Ama87I CYCGRG 1 cut(s) 2513
Aor13HI TCCGGA 1 cut(s) 2022
AoxI GGCC 3 cut(s) 278, 452, 829
ApeKI GCWGC 7 cut(s) 89, 92, 95, 710, 1412, 1946, 2433
ApoI RAATTY 2 cut(s) 373, 2164
ArsI GACNNNNNNTTYG 4 cut(s) 1263, 1295, 1597, 1629
AseI ATTAAT 1 cut(s) 789
Asp700I GAANNNNTTC 2 cut(s) 1018, 1449
Asp718I GGTACC 1 cut(s) 1468
AspA2I CCTAGG 1 cut(s) 1555
AspLEI GCGC 2 cut(s) 730, 1924
AspS9I GGNCC 6 cut(s) 201, 452, 623, 830, 1559, 1802
AsuC2I CCSGG 5 cut(s) 1595, 2157, 2448, 2514, 2515
AsuHPI GGTGA 7 cut(s) 196, 409, 430, 908, 1091, 1658, 2068
AsuII TTCGAA 1 cut(s) 2286
AvaI CYCGRG 1 cut(s) 2513
AvaII GGWCC 4 cut(s) 201, 623, 1559, 1802
AvrII CCTAGG 1 cut(s) 1555
AxyI CCTNAGG 1 cut(s) 443
BanI GGYRCC 1 cut(s) 1468
BanII GRGCYC 3 cut(s) 1159, 1728, 1966
BbsI GAAGAC 2 cut(s) 930, 1687
Bbv12I GWGCWC 3 cut(s) 266, 1159, 1791
BbvCI CCTCAGC 1 cut(s) 500
BbvI GCAGC 7 cut(s) 76, 79, 82, 722, 1399, 1933, 2445
BccI CCATC 5 cut(s) 95, 188, 212, 956, 1527
BciT130I CCWGG 5 cut(s) 828, 1161, 1221, 1247, 1823
BclI TGATCA 1 cut(s) 210
BcnI CCSGG 5 cut(s) 1595, 2157, 2448, 2514, 2515
BcoDI GTCTC 6 cut(s) 55, 150, 810, 1443, 2226, 2554
BfaI CTAG 4 cut(s) 62, 1545, 1556, 2139
BfmI CTRYAG 2 cut(s) 96, 1947
BfuAI ACCTGC 2 cut(s) 189, 1456
BglII AGATCT 1 cut(s) 2141
BisI GCNGC 9 cut(s) 90, 93, 96, 711, 1413, 1947, 2078, 2331, 2434
BlnI CCTAGG 1 cut(s) 1555
BlsI GCNGC 9 cut(s) 91, 94, 97, 712, 1414, 1948, 2079, 2332, 2435
Bme18I GGWCC 4 cut(s) 201, 623, 1559, 1802
BmeT110I CYCGRG 1 cut(s) 2513
BmgT120I GGNCC 6 cut(s) 201, 452, 623, 830, 1559, 1802
BmiI GGNNCC 9 cut(s) 344, 476, 510, 967, 1086, 1470, 1660, 1804, 2452
BmrI ACTGGG 2 cut(s) 1438, 2499
BmuI ACTGGG 2 cut(s) 1438, 2499
BoxI GACNNNNGTC 2 cut(s) 1349, 1557
BpiI GAAGAC 2 cut(s) 930, 1687
BpmI CTGGAG 2 cut(s) 1182, 1425
Bpu10I CCTNAGC 3 cut(s) 500, 639, 2479
Bpu14I TTCGAA 1 cut(s) 2286
BpuEI CTTGAG 4 cut(s) 1088, 1154, 2103, 2330
BpuMI CCSGG 5 cut(s) 1595, 2157, 2448, 2514, 2515
BsaI GGTCTC 1 cut(s) 55
BsaWI WCCGGW 1 cut(s) 2022
Bsc4I CCNNNNNNNGG 6 cut(s) 362, 620, 1094, 1174, 1616, 1772
Bse118I RCCGGY 1 cut(s) 85
Bse1I ACTGG 8 cut(s) 140, 782, 875, 1433, 1472, 2035, 2087, 2505
Bse21I CCTNAGG 1 cut(s) 443
Bse3DI GCAATG 4 cut(s) 805, 927, 1632, 1739
BseAI TCCGGA 1 cut(s) 2022
BseBI CCWGG 5 cut(s) 828, 1161, 1221, 1247, 1823
BseGI GGATG 9 cut(s) 459, 775, 967, 1326, 1538, 2031, 2091, 2197, 2263
BseLI CCNNNNNNNGG 6 cut(s) 362, 620, 1094, 1174, 1616, 1772
BseMI GCAATG 4 cut(s) 805, 927, 1632, 1739
BseMII CTCAG 8 cut(s) 304, 457, 491, 653, 855, 893, 1376, 1902
BseNI ACTGG 8 cut(s) 140, 782, 875, 1433, 1472, 2035, 2087, 2505
BseXI GCAGC 7 cut(s) 76, 79, 82, 722, 1399, 1933, 2445
BseYI CCCAGC 1 cut(s) 1863
BsgI GTGCAG 2 cut(s) 722, 1484
Bsh1236I CGCG 2 cut(s) 225, 1679
BshFI GGCC 3 cut(s) 280, 454, 831
BshNI GGYRCC 1 cut(s) 1468
BsiHKAI GWGCWC 3 cut(s) 266, 1159, 1791
BsiHKCI CYCGRG 1 cut(s) 2513
BsiSI CCGG 7 cut(s) 86, 1595, 1800, 2023, 2156, 2447, 2514
BslFI GGGAC 5 cut(s) 131, 704, 1773, 1881, 2486
BslI CCNNNNNNNGG 6 cut(s) 362, 620, 1094, 1174, 1616, 1772
BsmAI GTCTC 6 cut(s) 55, 150, 810, 1443, 2226, 2554
BsmFI GGGAC 5 cut(s) 131, 704, 1773, 1881, 2486
BsmI GAATGC 2 cut(s) 563, 2282
BsnI GGCC 3 cut(s) 280, 454, 831
Bso31I GGTCTC 1 cut(s) 55
BsoBI CYCGRG 1 cut(s) 2513
Bsp119I TTCGAA 1 cut(s) 2286
Bsp1286I GDGCHC 5 cut(s) 266, 1159, 1728, 1791, 1966
Bsp13I TCCGGA 1 cut(s) 2022
Bsp1407I TGTACA 1 cut(s) 2495
Bsp19I CCATGG 2 cut(s) 746, 991
BspACI CCGC 4 cut(s) 1679, 2077, 2102, 2331
BspANI GGCC 3 cut(s) 280, 454, 831
BspCNI CTCAG 8 cut(s) 303, 456, 492, 652, 856, 892, 1375, 1903
BspEI TCCGGA 1 cut(s) 2022
BspFNI CGCG 2 cut(s) 225, 1679
BspHI TCATGA 2 cut(s) 207, 349
BspLI GGNNCC 9 cut(s) 344, 476, 510, 967, 1086, 1470, 1660, 1804, 2452
BspMAI CTGCAG 2 cut(s) 100, 1951
BspMI ACCTGC 2 cut(s) 189, 1456
BspPI GGATC 7 cut(s) 131, 758, 773, 893, 2209, 2528, 2563
BspT104I TTCGAA 1 cut(s) 2286
BspT107I GGYRCC 1 cut(s) 1468
BspTNI GGTCTC 1 cut(s) 55
BsrDI GCAATG 4 cut(s) 805, 927, 1632, 1739
BsrFI RCCGGY 1 cut(s) 85
BsrGI TGTACA 1 cut(s) 2495
BsrI ACTGG 8 cut(s) 140, 782, 875, 1433, 1472, 2035, 2087, 2505
BssAI RCCGGY 1 cut(s) 85
BssT1I CCWWGG 4 cut(s) 746, 957, 991, 1555
Bst2UI CCWGG 5 cut(s) 828, 1161, 1221, 1247, 1823
Bst6I CTCTTC 1 cut(s) 2463
BstAUI TGTACA 1 cut(s) 2495
BstBI TTCGAA 1 cut(s) 2286
BstC8I GCNNGC 5 cut(s) 87, 672, 1951, 2320, 2389
BstDSI CCRYGG 5 cut(s) 257, 478, 746, 991, 1347
BstEII GGTNACC 1 cut(s) 1097
BstENI CCTNNNNNAGG 1 cut(s) 360
BstF5I GGATG 9 cut(s) 459, 775, 967, 1326, 1538, 2031, 2091, 2197, 2263
BstFNI CGCG 2 cut(s) 225, 1679
BstHHI GCGC 2 cut(s) 730, 1924
BstMAI GTCTC 6 cut(s) 55, 150, 810, 1443, 2226, 2554
BstMWI GCNNNNNNNGC 9 cut(s) 95, 569, 719, 847, 1084, 1199, 1247, 1887, 2336
BstNI CCWGG 5 cut(s) 828, 1161, 1221, 1247, 1823
BstNSI RCATGY 1 cut(s) 2496
BstPAI GACNNNNGTC 2 cut(s) 1349, 1557
BstPI GGTNACC 1 cut(s) 1097
BstSFI CTRYAG 2 cut(s) 96, 1947
BstUI CGCG 2 cut(s) 225, 1679
BstV1I GCAGC 7 cut(s) 76, 79, 82, 722, 1399, 1933, 2445
BstV2I GAAGAC 2 cut(s) 930, 1687
BstX2I RGATCY 3 cut(s) 136, 2141, 2214
BstYI RGATCY 3 cut(s) 136, 2141, 2214
Bsu36I CCTNAGG 1 cut(s) 443
BsuRI GGCC 3 cut(s) 280, 454, 831
BtgI CCRYGG 5 cut(s) 257, 478, 746, 991, 1347
BtsCI GGATG 9 cut(s) 459, 775, 967, 1326, 1538, 2031, 2091, 2197, 2263
BtsI GCAGTG 1 cut(s) 710
BtsIMutI CAGTG 8 cut(s) 273, 710, 808, 1479, 1761, 1908, 2080, 2512
BveI ACCTGC 2 cut(s) 189, 1456
Cac8I GCNNGC 5 cut(s) 87, 672, 1951, 2320, 2389
CciI TCATGA 2 cut(s) 207, 349
CfoI GCGC 2 cut(s) 730, 1924
Cfr10I RCCGGY 1 cut(s) 85
Cfr13I GGNCC 6 cut(s) 201, 452, 623, 830, 1559, 1802
Cfr9I CCCGGG 1 cut(s) 2513
Csp6I GTAC 8 cut(s) 655, 883, 988, 1469, 1975, 2128, 2397, 2496
CspCI CAANNNNNGTGG 2 cut(s) 1531, 1566
CviQI GTAC 8 cut(s) 655, 883, 988, 1469, 1975, 2128, 2397, 2496
Eam1104I CTCTTC 1 cut(s) 2463
EarI CTCTTC 1 cut(s) 2463
Ecl136II GAGCTC 1 cut(s) 1157
Eco130I CCWWGG 4 cut(s) 746, 957, 991, 1555
Eco24I GRGCYC 3 cut(s) 1159, 1728, 1966
Eco31I GGTCTC 1 cut(s) 55
Eco47I GGWCC 4 cut(s) 201, 623, 1559, 1802
Eco53kI GAGCTC 1 cut(s) 1157
Eco57I CTGAAG 2 cut(s) 2019, 2424
Eco81I CCTNAGG 1 cut(s) 443
Eco88I CYCGRG 1 cut(s) 2513
Eco91I GGTNACC 1 cut(s) 1097
EcoICRI GAGCTC 1 cut(s) 1157
EcoNI CCTNNNNNAGG 1 cut(s) 360
EcoO109I RGGNCCY 1 cut(s) 830
EcoO65I GGTNACC 1 cut(s) 1097
EcoRII CCWGG 5 cut(s) 826, 1159, 1219, 1245, 1821
EcoT14I CCWWGG 4 cut(s) 746, 957, 991, 1555
EcoT22I ATGCAT 2 cut(s) 2265, 2284
EcoT38I GRGCYC 3 cut(s) 1159, 1728, 1966
ErhI CCWWGG 4 cut(s) 746, 957, 991, 1555
FalI AAGNNNNNCTT 4 cut(s) 1044, 1076, 1267, 1299
FaqI GGGAC 5 cut(s) 131, 704, 1773, 1881, 2486
FauNDI CATATG 3 cut(s) 190, 1232, 1564
FbaI TGATCA 1 cut(s) 210
Fnu4HI GCNGC 9 cut(s) 90, 93, 96, 711, 1413, 1947, 2078, 2331, 2434
FokI GGATG 9 cut(s) 446, 782, 974, 1313, 1545, 2038, 2098, 2204, 2250
FriOI GRGCYC 3 cut(s) 1159, 1728, 1966
Fsp4HI GCNGC 9 cut(s) 90, 93, 96, 711, 1413, 1947, 2078, 2331, 2434
FspBI CTAG 4 cut(s) 62, 1545, 1556, 2139
FspI TGCGCA 1 cut(s) 729
GlaI GCGC 2 cut(s) 729, 1923
GluI GCNGC 9 cut(s) 90, 93, 96, 711, 1413, 1947, 2078, 2331, 2434
GsaI CCCAGC 1 cut(s) 1867
GsuI CTGGAG 2 cut(s) 1182, 1425
HaeIII GGCC 3 cut(s) 280, 454, 831
HapII CCGG 7 cut(s) 86, 1595, 1800, 2023, 2156, 2447, 2514
HhaI GCGC 2 cut(s) 730, 1924
Hin6I GCGC 2 cut(s) 728, 1922
HinP1I GCGC 2 cut(s) 728, 1922
HincII GTYRAC 1 cut(s) 2227
HindII GTYRAC 1 cut(s) 2227
HindIII AAGCTT 1 cut(s) 2389
HinfI GANTC 8 cut(s) 322, 415, 1126, 1164, 1477, 1583, 1605, 1761
HpaII CCGG 7 cut(s) 86, 1595, 1800, 2023, 2156, 2447, 2514
HphI GGTGA 7 cut(s) 196, 409, 430, 908, 1091, 1658, 2068
Hpy166II GTNNAC 5 cut(s) 657, 1539, 1908, 2227, 2255
Hpy188I TCNGA 4 cut(s) 55, 532, 1604, 1658
Hpy8I GTNNAC 5 cut(s) 657, 1539, 1908, 2227, 2255
Hpy99I CGWCG 1 cut(s) 740
HpyCH4IV ACGT 3 cut(s) 75, 735, 2274
HpyF10VI GCNNNNNNNGC 9 cut(s) 95, 569, 719, 847, 1084, 1199, 1247, 1887, 2336
HpySE526I ACGT 3 cut(s) 75, 735, 2274
HspAI GCGC 2 cut(s) 728, 1922
Kpn2I TCCGGA 1 cut(s) 2022
KpnI GGTACC 1 cut(s) 1472
KroI GCCGGC 1 cut(s) 85
KroNI GCCGGC 1 cut(s) 87
Ksp22I TGATCA 1 cut(s) 210
Lsp1109I GCAGC 7 cut(s) 76, 79, 82, 722, 1399, 1933, 2445
MaeI CTAG 4 cut(s) 62, 1545, 1556, 2139
MaeII ACGT 3 cut(s) 75, 735, 2274
MaeIII GTNAC 8 cut(s) 184, 436, 1097, 1279, 1374, 1619, 1708, 2507
MboII GAAGA 9 cut(s) 146, 175, 278, 595, 935, 1023, 1687, 2480, 2515
MfeI CAATTG 1 cut(s) 1842
MflI RGATCY 3 cut(s) 136, 2141, 2214
MhlI GDGCHC 5 cut(s) 266, 1159, 1728, 1791, 1966
MluCI AATT 7 cut(s) 246, 373, 1357, 1449, 1842, 2164, 2310
MlyI GAGTC 2 cut(s) 1173, 1755
MmeI TCCRAC 2 cut(s) 563, 1478
Mph1103I ATGCAT 2 cut(s) 2265, 2284
MroI TCCGGA 1 cut(s) 2022
MroNI GCCGGC 1 cut(s) 85
MroXI GAANNNNTTC 2 cut(s) 1018, 1449
MseI TTAA 5 cut(s) 78, 789, 1356, 2066, 2277
MslI CAYNNNNRTG 2 cut(s) 1754, 2277
MspA1I CMGCKG 1 cut(s) 2436
MspI CCGG 7 cut(s) 86, 1595, 1800, 2023, 2156, 2447, 2514
MunI CAATTG 1 cut(s) 1842
Mva1269I GAATGC 2 cut(s) 563, 2282
MvaI CCWGG 5 cut(s) 828, 1161, 1221, 1247, 1823
MvnI CGCG 2 cut(s) 225, 1679
MwoI GCNNNNNNNGC 9 cut(s) 95, 569, 719, 847, 1084, 1199, 1247, 1887, 2336
NaeI GCCGGC 1 cut(s) 87
NciI CCSGG 5 cut(s) 1595, 2157, 2448, 2514, 2515
NcoI CCATGG 2 cut(s) 746, 991
NdeI CATATG 3 cut(s) 190, 1232, 1564
NgoMIV GCCGGC 1 cut(s) 85
NlaIV GGNNCC 9 cut(s) 344, 476, 510, 967, 1086, 1470, 1660, 1804, 2452
NmeAIII GCCGAG 1 cut(s) 2149
NmuCI GTSAC 8 cut(s) 184, 436, 1097, 1279, 1374, 1619, 1708, 2507
NsbI TGCGCA 1 cut(s) 729
NsiI ATGCAT 2 cut(s) 2265, 2284
NspI RCATGY 1 cut(s) 2496
NspV TTCGAA 1 cut(s) 2286
PagI TCATGA 2 cut(s) 207, 349
PcsI WCGNNNNNNNCGW 2 cut(s) 222, 1629
PctI GAATGC 2 cut(s) 563, 2282
PdiI GCCGGC 1 cut(s) 87
PdmI GAANNNNTTC 2 cut(s) 1018, 1449
PfeI GAWTC 6 cut(s) 322, 415, 1126, 1477, 1583, 1605
PflMI CCANNNNNTGG 2 cut(s) 1616, 1772
PfoI TCCNGGA 2 cut(s) 1159, 2446
PkrI GCNGC 9 cut(s) 91, 94, 97, 712, 1414, 1948, 2079, 2332, 2435
PleI GAGTC 2 cut(s) 1172, 1755
PpsI GAGTC 2 cut(s) 1172, 1755
PshAI GACNNNNGTC 2 cut(s) 1349, 1557
PshBI ATTAAT 1 cut(s) 789
Psp124BI GAGCTC 1 cut(s) 1159
Psp1406I AACGTT 1 cut(s) 75
Psp6I CCWGG 5 cut(s) 826, 1159, 1219, 1245, 1821
PspEI GGTNACC 1 cut(s) 1097
PspFI CCCAGC 1 cut(s) 1863
PspGI CCWGG 5 cut(s) 826, 1159, 1219, 1245, 1821
PspN4I GGNNCC 9 cut(s) 344, 476, 510, 967, 1086, 1470, 1660, 1804, 2452
PspPI GGNCC 6 cut(s) 201, 452, 623, 830, 1559, 1802
PstI CTGCAG 2 cut(s) 100, 1951
PsuI RGATCY 3 cut(s) 136, 2141, 2214
PvuII CAGCTG 1 cut(s) 2436
RsaI GTAC 8 cut(s) 656, 884, 989, 1470, 1976, 2129, 2398, 2497
RsaNI GTAC 8 cut(s) 655, 883, 988, 1469, 1975, 2128, 2397, 2496
RseI CAYNNNNRTG 2 cut(s) 1754, 2277
SacI GAGCTC 1 cut(s) 1159
SaqAI TTAA 5 cut(s) 78, 789, 1356, 2066, 2277
SatI GCNGC 9 cut(s) 90, 93, 96, 711, 1413, 1947, 2078, 2331, 2434
Sau96I GGNCC 6 cut(s) 201, 452, 623, 830, 1559, 1802
SchI GAGTC 2 cut(s) 1173, 1755
SduI GDGCHC 5 cut(s) 266, 1159, 1728, 1791, 1966
SfcI CTRYAG 2 cut(s) 96, 1947
SfuI TTCGAA 1 cut(s) 2286
SinI GGWCC 4 cut(s) 201, 623, 1559, 1802
SmaI CCCGGG 1 cut(s) 2515
SmiMI CAYNNNNRTG 2 cut(s) 1754, 2277
SmlI CTYRAG 4 cut(s) 1103, 1169, 2118, 2345
SmoI CTYRAG 4 cut(s) 1103, 1169, 2118, 2345
Sse9I AATT 7 cut(s) 246, 373, 1357, 1449, 1842, 2164, 2310
SsiI CCGC 4 cut(s) 1679, 2077, 2102, 2331
SspMI CTAG 4 cut(s) 62, 1545, 1556, 2139
SstI GAGCTC 1 cut(s) 1159
StyI CCWWGG 4 cut(s) 746, 957, 991, 1555
TaiI ACGT 3 cut(s) 78, 738, 2277
TaqI TCGA 7 cut(s) 378, 946, 1039, 1269, 1402, 1698, 2286
TasI AATT 7 cut(s) 246, 373, 1357, 1449, 1842, 2164, 2310
TatI WGTACW 3 cut(s) 654, 882, 2495
TauI GCSGC 2 cut(s) 2080, 2333
TfiI GAWTC 6 cut(s) 322, 415, 1126, 1477, 1583, 1605
Tru1I TTAA 5 cut(s) 78, 789, 1356, 2066, 2277
Tru9I TTAA 5 cut(s) 78, 789, 1356, 2066, 2277
TscAI CASTG 8 cut(s) 273, 710, 815, 1479, 1761, 1915, 2087, 2512
TseFI GTSAC 8 cut(s) 184, 436, 1097, 1279, 1374, 1619, 1708, 2507
TseI GCWGC 7 cut(s) 89, 92, 95, 710, 1412, 1946, 2433
Tsp45I GTSAC 8 cut(s) 184, 436, 1097, 1279, 1374, 1619, 1708, 2507
TspGWI ACGGA 5 cut(s) 231, 274, 495, 515, 1683
TspMI CCCGGG 1 cut(s) 2513
TspRI CASTG 8 cut(s) 273, 710, 815, 1479, 1761, 1915, 2087, 2512
Van91I CCANNNNNTGG 2 cut(s) 1616, 1772
VpaK11BI GGWCC 4 cut(s) 201, 623, 1559, 1802
VspI ATTAAT 1 cut(s) 789
XagI CCTNNNNNAGG 1 cut(s) 360
XapI RAATTY 2 cut(s) 373, 2164
XceI RCATGY 1 cut(s) 2496
XcmI CCANNNNNNNNNTGG 1 cut(s) 1819
XmaI CCCGGG 1 cut(s) 2513
XmaJI CCTAGG 1 cut(s) 1555
XmnI GAANNNNTTC 2 cut(s) 1018, 1449
XspI CTAG 4 cut(s) 62, 1545, 1556, 2139
Zsp2I ATGCAT 2 cut(s) 2265, 2284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.