Rw6G001570

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0002763
Physical Location & Seq
Forward (+)
2411497 .. 2416069
4573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G001570.1

Sequence Viewer

Length: 2604 bp
ATGGTGGTACCAAGCCAAAGGGTGCTCTTGATATCTTTTCTACTTTTGTTGGTCTCGACCACATTAATCCATGCCAAAGGTGACAAGCCGAAACCAGAAGGTGGTGGCGTGGCTGCTGGGGACGGTGGGGATGGCGGTGACGGAGAGGTTGCCGGGGACACTACAGATGACGGGGCAGTACAGGGAGGAAAGAAGCAGCTTGCACACGGTCCCGTGACGTATGATGCCAGGTCTTTGATCATTAATGGGAAACATGAGCTTCTATTTTCTGGTTCAATTCATTATCCACGTAGCCCGGTGGAAATGTGGCCTGATATTCTGAAGAAGGCTAAACAAGGAGGTTTGAATGTGATTCAAACTTATGTGTTTTGGAACATTCATGAGCCTGTGCAAGGCAAGTGGGAGTTCGAGGGAAACTATGATTTGGTGAAGTTTATTAGGTTGATCCAGGAGAATGGAATGTATGCTACCCTCAGGGTTGGACCATTCATCCAAGCAGAATGGAACCATGGAGGTCTTCCATATTGGCTGAGAGAGGTCCCAGGAATCACTTTCCGATCAGATAATGAACCATACAAGAAACACATGGAGCAATATGTGAACAAGATAATGAAGATGATGAAGGATGAAAAGCTATTTTCTCCACAAGGAGGTCCAATCATCTTAGCTCAGATTGAGAATGAGTACAATCAAATCCAACTTGCATATAGAGAGTTGGGAGATACATATGTGCAGTGGGCAGCAAAGCTGGCAGTTGGGCAGAATATTGGAGTGCCATGGATCATGTGCAAGCAAAAGGATGCTCCTGATCCGGTGATTAATACTTGCAACGGAAGGCATTGTGGAGATACTTTTTCAGGACCTAACAAACCCAACAAGCCTGCTCTATGGACTGAGAATTGGACAGCTCAGTACCGAGTATTTGGTGACCCTCCTTCACAAAGAGCAGCAGAAGACATTGCATTTTCAGTTGCTCGGTTTTTCTCCAAGGGTGGGGCTCTAACCAACTACTATATGTACCATGGTGGAACGAATTTTGGAAGAACAAGTGCTGTTTTCACAACAACTCGTTACTACGATGAGGCTCCTCTCGATGAATATGCTTTGCAAAGGGACCCGAAATGGAGTCACCTCAAGGACTTGCACAAGGCTATAAATCTATGCAAGAAAACTTTGCTTACTGGAACTCCCGGAGACCAAAGGTTGGGGGAAGAAACTGAGGTTCGTTTCTATGAGAAGCCAGGGACAGAGTGTGCTGCTTTCATAGCAAATAATCACTCGACCATGGAAGCAACCGTTAATTGGAGGGGACAGAAGTTTCTCCTGCCACCAGCTTCCATTAGCATCCTCCCCGACTGCAAGACTGTGGTCTTCAATACTCAACAAATTGTATCCCAACATAATTCAAGGAACTTTGTTACAGCTAGTAAAAAAATGAACTGGGAAATGTCTCCCGAACCCATTGCAACCGTCGCGCAAATCCCATCCAATAGTAGGGAAATGCAGGAGCTCTATCTCTTGCTAAAAGATACCACAGACTATGCATGGTACACAACTAGCATTGACCTAAGTCCGTATGACTTGCCGATGAAGGAGACTATCAGGCCAGTTGTACGAATTCCTAGTCTTGGTCATGCAATGGCTGTATTTGTCAATGATGAATATATTGGATCGCAACACGGTAGCCACGCAGAAAAAGGTTTCGTGTTCGAGGCACCTGCTAAAATGAAGCTAGGGGCGAACCATATCTCTCTGCTTTGCATTGTAGTGGGACTCCCAGATAGTGGAGCGTACATGGAGCACAGGTATGCAGGGCCTCGTTCTGTAACAATCCTAGGATTGAACACCGGAACAATCGACCTGTCCGCAAATGGCTGGGGACATAGGGCTGGCCTGAATGGTGAAAAGCTCGAAGTGTACACTGAGAAGGGAGCTCAGAAAGTGAAATGGACTAAGGCAACAGGTAAAGGAAGGGCTCTCACATGGTACAAGACAAGTTTTGATGCTCCTTCAGGAAGAGACCCTTGTGCTATTCGAATGACCGGTATGGGCAAGGGAATGGTATGGGTGAATGGTATAAGCATCGGCCGTCATTGGATGTCCTTCCTCTCTCCTCTTGGACAACCTACTCAAATCGAGTACCACATCCCAAGAGCCTTCATTAAGCCTAAGAATTTCCTTGTTGTATTGGAGGAGGAGCCAGCAGACCCAAAAGACATTGAGATCGTAACAGTCAACAGAGACACCATCTGCAGCCTTATTAAACAGGACTATCCGGGACATGTTAAGTACTGGGATAGAAAAGGCGGCAAGCTCACAACAGCTCCTGGAAAAAGTCTCCAACCATCAGCTCTCCTCAACTGTCCAAACTACAAAAAGATCAGTGCTATTGAATTCGCGGGCTATGGCAATCCTGACGGCTTCTGTGGAGCCTTTAAATTGGGACAATGCAATGCTCCTGAAGCCAAGAAGGTCGTCGAAGATCATTGCTTGGGAAAGATTTCATGTACAGTCCCAAATGACCCCAGCCTCTTCTTCAAGTCTGGTGATCCTTGCCCTGGTATCAAGAAGACACTAGCTATCCAAGCAGCGTGCAGCTTTTAA

Protein Analysis

867

Amino Acids

96.12

Weight (kDa)

8.45

Isoelectric Point (pI)

36.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 78 - 384 2.6e-116 Glycosyl hydrolases family 35
Glyco_hydro_42 PF02449 99 - 231 4.2e-06 Beta-galactosidase
GHD PF17834 408 - 463 4.2e-20 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 660 - 724 3.4e-22 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 786 - 865 3.5e-17 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1726
Acc36I ACCTGC 1 cut(s) 1726
Acc65I GGTACC 1 cut(s) 7
AccB1I GGYRCC 2 cut(s) 7, 1714
AccB7I CCANNNNNTGG 3 cut(s) 101, 1204, 1784
AccII CGCG 2 cut(s) 1475, 2399
AciI CCGC 4 cut(s) 135, 1866, 2307, 2399
AclWI GGATC 5 cut(s) 439, 788, 803, 1678, 2543
AcoI YGGCCR 1 cut(s) 2086
AcsI RAATTY 4 cut(s) 1033, 1617, 2173, 2393
AcuI CTGAAG 3 cut(s) 341, 1995, 2481
AflIII ACRYGT 1 cut(s) 2281
AgeI ACCGGT 1 cut(s) 2042
AgsI TTSAA 8 cut(s) 276, 346, 356, 1375, 1407, 1843, 2393, 2539
AjnI CCWGG 6 cut(s) 227, 447, 541, 1240, 2326, 2557
Alw21I GWGCWC 4 cut(s) 27, 1512, 1803, 1936
Alw26I GTCTC 7 cut(s) 58, 1188, 1455, 1589, 2013, 2235, 2342
AlwI GGATC 5 cut(s) 439, 788, 803, 1678, 2543
AlwNI CAGNNNCTG 1 cut(s) 2327
AoxI GGCC 5 cut(s) 308, 1604, 1814, 1891, 2086
ApeKI GCWGC 8 cut(s) 113, 196, 740, 947, 1256, 2253, 2588, 2595
ApoI RAATTY 4 cut(s) 1033, 1617, 2173, 2393
ArsI GACNNNNNNTTYG 2 cut(s) 1609, 1641
AseI ATTAAT 3 cut(s) 65, 243, 819
AsiGI ACCGGT 1 cut(s) 2042
Asp700I GAANNNNTTC 1 cut(s) 2500
Asp718I GGTACC 1 cut(s) 7
AspA2I CCTAGG 1 cut(s) 1834
AspLEI GCGC 1 cut(s) 1477
AspS9I GGNCC 7 cut(s) 209, 482, 538, 653, 860, 1114, 1814
AsuC2I CCSGG 4 cut(s) 154, 296, 1191, 2277
AsuHPI GGTGA 9 cut(s) 92, 149, 439, 826, 938, 1121, 1913, 2080, 2558
AsuII TTCGAA 1 cut(s) 2035
AvaII GGWCC 6 cut(s) 209, 482, 538, 653, 860, 1114
AvrII CCTAGG 1 cut(s) 1834
AxyI CCTNAGG 1 cut(s) 473
BaeI ACNNNNGTAYC 2 cut(s) 714, 747
BanI GGYRCC 2 cut(s) 7, 1714
BanII GRGCYC 4 cut(s) 1000, 1512, 1936, 1978
BbsI GAAGAC 4 cut(s) 509, 960, 1363, 2576
Bbv12I GWGCWC 4 cut(s) 27, 1512, 1803, 1936
BbvI GCAGC 7 cut(s) 100, 208, 752, 959, 1243, 2265, 2600
BccI CCATC 4 cut(s) 125, 1492, 2255, 2353
BceAI ACGGC 2 cut(s) 2073, 2434
BcgI CGANNNNNNTGC 6 cut(s) 1082, 1116, 1445, 1479, 1700, 1734
BciT130I CCWGG 6 cut(s) 229, 449, 543, 1242, 2328, 2559
BciVI GTATCC 1 cut(s) 1402
BclI TGATCA 1 cut(s) 237
BcnI CCSGG 4 cut(s) 154, 296, 1191, 2277
BcoDI GTCTC 7 cut(s) 58, 1188, 1455, 1589, 2013, 2235, 2342
BfaI CTAG 6 cut(s) 1425, 1557, 1623, 1733, 1835, 2576
BfmI CTRYAG 2 cut(s) 162, 2251
BfuAI ACCTGC 1 cut(s) 1726
BfuI GTATCC 1 cut(s) 1402
BisI GCNGC 9 cut(s) 114, 197, 741, 948, 1257, 2254, 2308, 2589, 2596
BlnI CCTAGG 1 cut(s) 1834
BlsI GCNGC 9 cut(s) 115, 198, 742, 949, 1258, 2255, 2309, 2590, 2597
BmcAI AGTACT 1 cut(s) 2291
Bme18I GGWCC 6 cut(s) 209, 482, 538, 653, 860, 1114
BmgT120I GGNCC 7 cut(s) 209, 482, 538, 653, 860, 1114, 1814
BmrI ACTGGG 2 cut(s) 1450, 2302
BmsI GCATC 5 cut(s) 214, 790, 1353, 1993, 2091
BmuI ACTGGG 2 cut(s) 1450, 2302
BoxI GACNNNNGTC 2 cut(s) 1367, 1569
BpiI GAAGAC 4 cut(s) 509, 960, 1363, 2576
Bpu14I TTCGAA 1 cut(s) 2035
BpuEI CTTGAG 1 cut(s) 1118
BpuMI CCSGG 4 cut(s) 154, 296, 1191, 2277
BsaAI YACGTR 1 cut(s) 290
BsaI GGTCTC 3 cut(s) 58, 1188, 2013
BsaWI WCCGGW 3 cut(s) 811, 1847, 2042
BsaXI ACNNNNNCTCC 6 cut(s) 1171, 1201, 1790, 1820, 2308, 2338
Bse118I RCCGGY 1 cut(s) 2042
Bse1I ACTGG 4 cut(s) 1186, 1445, 1607, 2297
Bse21I CCTNAGG 1 cut(s) 473
Bse3DI GCAATG 5 cut(s) 957, 1461, 1644, 2458, 2485
BseBI CCWGG 6 cut(s) 229, 449, 543, 1242, 2328, 2559
BseGI GGATG 8 cut(s) 136, 489, 631, 805, 1344, 1484, 2103, 2145
BseMI GCAATG 5 cut(s) 957, 1461, 1644, 2458, 2485
BseMII CTCAG 8 cut(s) 487, 521, 683, 885, 923, 1209, 1914, 1949
BseNI ACTGG 4 cut(s) 1186, 1445, 1607, 2297
BseRI GAGGAG 5 cut(s) 1077, 2103, 2207, 2210, 2345
BseX3I CGGCCG 1 cut(s) 2086
BseXI GCAGC 7 cut(s) 100, 208, 752, 959, 1243, 2265, 2600
BseYI CCCAGC 3 cut(s) 116, 1875, 2525
BsgI GTGCAG 1 cut(s) 752
Bsh1236I CGCG 2 cut(s) 1475, 2399
Bsh1285I CGRYCG 1 cut(s) 2089
BshFI GGCC 5 cut(s) 310, 1606, 1816, 1893, 2088
BshNI GGYRCC 2 cut(s) 7, 1714
BshTI ACCGGT 1 cut(s) 2042
BsiEI CGRYCG 1 cut(s) 2089
BsiHKAI GWGCWC 4 cut(s) 27, 1512, 1803, 1936
BsiSI CCGG 7 cut(s) 153, 296, 812, 1191, 1848, 2043, 2276
BsmAI GTCTC 7 cut(s) 58, 1188, 1455, 1589, 2013, 2235, 2342
BsnI GGCC 5 cut(s) 310, 1606, 1816, 1893, 2088
Bso31I GGTCTC 3 cut(s) 58, 1188, 2013
Bsp119I TTCGAA 1 cut(s) 2035
Bsp1286I GDGCHC 6 cut(s) 27, 1000, 1512, 1803, 1936, 1978
Bsp1407I TGTACA 2 cut(s) 1917, 2507
Bsp19I CCATGG 4 cut(s) 508, 776, 1021, 1284
BspACI CCGC 4 cut(s) 135, 1866, 2307, 2399
BspANI GGCC 5 cut(s) 310, 1606, 1816, 1893, 2088
BspCNI CTCAG 8 cut(s) 486, 522, 682, 886, 922, 1210, 1915, 1948
BspFNI CGCG 2 cut(s) 1475, 2399
BspHI TCATGA 1 cut(s) 379
BspMAI CTGCAG 1 cut(s) 2255
BspMI ACCTGC 1 cut(s) 1726
BspPI GGATC 5 cut(s) 439, 788, 803, 1678, 2543
BspT104I TTCGAA 1 cut(s) 2035
BspT107I GGYRCC 2 cut(s) 7, 1714
BspTNI GGTCTC 3 cut(s) 58, 1188, 2013
BsrDI GCAATG 5 cut(s) 957, 1461, 1644, 2458, 2485
BsrFI RCCGGY 1 cut(s) 2042
BsrGI TGTACA 2 cut(s) 1917, 2507
BsrI ACTGG 4 cut(s) 1186, 1445, 1607, 2297
BssAI RCCGGY 1 cut(s) 2042
BssT1I CCWWGG 6 cut(s) 508, 776, 987, 1021, 1284, 1834
Bst2UI CCWGG 6 cut(s) 229, 449, 543, 1242, 2328, 2559
Bst4CI ACNGT 9 cut(s) 125, 209, 1297, 1366, 1471, 1682, 2233, 2363, 2512
Bst6I CTCTTC 2 cut(s) 2011, 2537
BstAUI TGTACA 2 cut(s) 1917, 2507
BstBAI YACGTR 1 cut(s) 290
BstBI TTCGAA 1 cut(s) 2035
BstC8I GCNNGC 9 cut(s) 201, 750, 791, 882, 1891, 2202, 2312, 2401, 2593
BstDSI CCRYGG 4 cut(s) 508, 776, 1021, 1284
BstEII GGTNACC 1 cut(s) 926
BstENI CCTNNNNNAGG 1 cut(s) 390
BstF5I GGATG 8 cut(s) 136, 489, 631, 805, 1344, 1484, 2103, 2145
BstFNI CGCG 2 cut(s) 1475, 2399
BstHHI GCGC 1 cut(s) 1477
BstMAI GTCTC 7 cut(s) 58, 1188, 1455, 1589, 2013, 2235, 2342
BstMCI CGRYCG 1 cut(s) 2089
BstMWI GCNNNNNNNGC 5 cut(s) 749, 1265, 1472, 2462, 2585
BstNI CCWGG 6 cut(s) 229, 449, 543, 1242, 2328, 2559
BstNSI RCATGY 1 cut(s) 2285
BstPAI GACNNNNGTC 2 cut(s) 1367, 1569
BstPI GGTNACC 1 cut(s) 926
BstSFI CTRYAG 2 cut(s) 162, 2251
BstUI CGCG 2 cut(s) 1475, 2399
BstV1I GCAGC 7 cut(s) 100, 208, 752, 959, 1243, 2265, 2600
BstV2I GAAGAC 4 cut(s) 509, 960, 1363, 2576
BstXI CCANNNNNNTGG 1 cut(s) 455
BstZI CGGCCG 1 cut(s) 2086
Bsu36I CCTNAGG 1 cut(s) 473
BsuI GTATCC 1 cut(s) 1402
BsuRI GGCC 5 cut(s) 310, 1606, 1816, 1893, 2088
BtgI CCRYGG 4 cut(s) 508, 776, 1021, 1284
BtsCI GGATG 8 cut(s) 136, 489, 631, 805, 1344, 1484, 2103, 2145
BtsI GCAGTG 1 cut(s) 740
BtsIMutI CAGTG 3 cut(s) 740, 1920, 2389
BveI ACCTGC 1 cut(s) 1726
Cac8I GCNNGC 9 cut(s) 201, 750, 791, 882, 1891, 2202, 2312, 2401, 2593
CaiI CAGNNNCTG 1 cut(s) 2327
CciI TCATGA 1 cut(s) 379
CfoI GCGC 1 cut(s) 1477
Cfr10I RCCGGY 1 cut(s) 2042
Cfr13I GGNCC 7 cut(s) 209, 482, 538, 653, 860, 1114, 1814
CspAI ACCGGT 1 cut(s) 2042
CspCI CAANNNNNGTGG 2 cut(s) 380, 415
DraI TTTAAA 1 cut(s) 2437
EaeI YGGCCR 1 cut(s) 2086
EagI CGGCCG 1 cut(s) 2086
Eam1104I CTCTTC 2 cut(s) 2011, 2537
EarI CTCTTC 2 cut(s) 2011, 2537
Ecl136II GAGCTC 2 cut(s) 1510, 1934
EclXI CGGCCG 1 cut(s) 2086
Eco130I CCWWGG 6 cut(s) 508, 776, 987, 1021, 1284, 1834
Eco24I GRGCYC 4 cut(s) 1000, 1512, 1936, 1978
Eco31I GGTCTC 3 cut(s) 58, 1188, 2013
Eco32I GATATC 1 cut(s) 33
Eco47I GGWCC 6 cut(s) 209, 482, 538, 653, 860, 1114
Eco52I CGGCCG 1 cut(s) 2086
Eco53kI GAGCTC 2 cut(s) 1510, 1934
Eco57I CTGAAG 3 cut(s) 341, 1995, 2481
Eco81I CCTNAGG 1 cut(s) 473
Eco91I GGTNACC 1 cut(s) 926
EcoICRI GAGCTC 2 cut(s) 1510, 1934
EcoNI CCTNNNNNAGG 1 cut(s) 390
EcoO109I RGGNCCY 4 cut(s) 538, 860, 1114, 1814
EcoO65I GGTNACC 1 cut(s) 926
EcoRI GAATTC 2 cut(s) 1617, 2393
EcoRII CCWGG 6 cut(s) 227, 447, 541, 1240, 2326, 2557
EcoRV GATATC 1 cut(s) 33
EcoT14I CCWWGG 6 cut(s) 508, 776, 987, 1021, 1284, 1834
EcoT22I ATGCAT 1 cut(s) 1546
EcoT38I GRGCYC 4 cut(s) 1000, 1512, 1936, 1978
ErhI CCWWGG 6 cut(s) 508, 776, 987, 1021, 1284, 1834
FalI AAGNNNNNCTT 2 cut(s) 2008, 2040
FauI CCCGC 1 cut(s) 2392
FauNDI CATATG 1 cut(s) 727
FbaI TGATCA 1 cut(s) 237
Fnu4HI GCNGC 9 cut(s) 114, 197, 741, 948, 1257, 2254, 2308, 2589, 2596
FokI GGATG 8 cut(s) 143, 476, 638, 812, 1331, 1471, 2110, 2132
FriOI GRGCYC 4 cut(s) 1000, 1512, 1936, 1978
Fsp4HI GCNGC 9 cut(s) 114, 197, 741, 948, 1257, 2254, 2308, 2589, 2596
FspBI CTAG 6 cut(s) 1425, 1557, 1623, 1733, 1835, 2576
GlaI GCGC 1 cut(s) 1476
GluI GCNGC 9 cut(s) 114, 197, 741, 948, 1257, 2254, 2308, 2589, 2596
GsaI CCCAGC 3 cut(s) 120, 1879, 2529
HaeIII GGCC 5 cut(s) 310, 1606, 1816, 1893, 2088
HapII CCGG 7 cut(s) 153, 296, 812, 1191, 1848, 2043, 2276
HhaI GCGC 1 cut(s) 1477
Hin6I GCGC 1 cut(s) 1475
HinP1I GCGC 1 cut(s) 1475
HincII GTYRAC 1 cut(s) 2236
HindII GTYRAC 1 cut(s) 2236
HinfI GANTC 4 cut(s) 352, 546, 1126, 1773
HpaII CCGG 7 cut(s) 153, 296, 812, 1191, 1848, 2043, 2276
HphI GGTGA 9 cut(s) 92, 149, 439, 826, 938, 1121, 1913, 2080, 2558
Hpy166II GTNNAC 5 cut(s) 601, 1551, 1918, 1920, 2236
Hpy188I TCNGA 5 cut(s) 321, 557, 562, 672, 1938
Hpy8I GTNNAC 5 cut(s) 601, 1551, 1918, 1920, 2236
Hpy99I CGWCG 2 cut(s) 1475, 2480
HpyCH4III ACNGT 9 cut(s) 125, 209, 1297, 1366, 1471, 1682, 2233, 2363, 2512
HpyCH4IV ACGT 2 cut(s) 218, 289
HpyF10VI GCNNNNNNNGC 5 cut(s) 749, 1265, 1472, 2462, 2585
HpySE526I ACGT 2 cut(s) 218, 289
HspAI GCGC 1 cut(s) 1475
KflI GGGWCCC 1 cut(s) 1114
KpnI GGTACC 1 cut(s) 11
Ksp22I TGATCA 1 cut(s) 237
Lsp1109I GCAGC 7 cut(s) 100, 208, 752, 959, 1243, 2265, 2600
LweI GCATC 5 cut(s) 214, 790, 1353, 1993, 2091
MaeI CTAG 6 cut(s) 1425, 1557, 1623, 1733, 1835, 2576
MaeII ACGT 2 cut(s) 218, 289
MaeIII GTNAC 9 cut(s) 80, 137, 214, 926, 1070, 1127, 1417, 1825, 2227
MhlI GDGCHC 6 cut(s) 27, 1000, 1512, 1803, 1936, 1978
MlyI GAGTC 2 cut(s) 1135, 1767
MmeI TCCRAC 3 cut(s) 460, 721, 2365
Mph1103I ATGCAT 1 cut(s) 1546
MroXI GAANNNNTTC 1 cut(s) 2500
MseI TTAA 9 cut(s) 65, 243, 819, 1299, 2163, 2262, 2286, 2436, 2602
MslI CAYNNNNRTG 2 cut(s) 1766, 1806
MspI CCGG 7 cut(s) 153, 296, 812, 1191, 1848, 2043, 2276
MvaI CCWGG 6 cut(s) 229, 449, 543, 1242, 2328, 2559
MvnI CGCG 2 cut(s) 1475, 2399
MwoI GCNNNNNNNGC 5 cut(s) 749, 1265, 1472, 2462, 2585
NciI CCSGG 4 cut(s) 154, 296, 1191, 2277
NcoI CCATGG 4 cut(s) 508, 776, 1021, 1284
NdeI CATATG 1 cut(s) 727
NmuCI GTSAC 5 cut(s) 80, 137, 214, 926, 1127
NsiI ATGCAT 1 cut(s) 1546
NspI RCATGY 1 cut(s) 2285
NspV TTCGAA 1 cut(s) 2035
PagI TCATGA 1 cut(s) 379
PaqCI CACCTGC 1 cut(s) 1726
PciI ACATGT 1 cut(s) 2281
PdmI GAANNNNTTC 1 cut(s) 2500
PfeI GAWTC 2 cut(s) 352, 546
PflMI CCANNNNNTGG 3 cut(s) 101, 1204, 1784
PfoI TCCNGGA 4 cut(s) 447, 1189, 2275, 2326
PinAI ACCGGT 1 cut(s) 2042
PkrI GCNGC 9 cut(s) 115, 198, 742, 949, 1258, 2255, 2309, 2590, 2597
PleI GAGTC 2 cut(s) 1134, 1767
PpsI GAGTC 2 cut(s) 1134, 1767
Ppu21I YACGTR 1 cut(s) 290
PpuMI RGGWCCY 3 cut(s) 538, 860, 1114
PscI ACATGT 1 cut(s) 2281
PshAI GACNNNNGTC 2 cut(s) 1367, 1569
PshBI ATTAAT 3 cut(s) 65, 243, 819
Psp124BI GAGCTC 2 cut(s) 1512, 1936
Psp5II RGGWCCY 3 cut(s) 538, 860, 1114
Psp6I CCWGG 6 cut(s) 227, 447, 541, 1240, 2326, 2557
PspEI GGTNACC 1 cut(s) 926
PspFI CCCAGC 3 cut(s) 116, 1875, 2525
PspGI CCWGG 6 cut(s) 227, 447, 541, 1240, 2326, 2557
PspPI GGNCC 7 cut(s) 209, 482, 538, 653, 860, 1114, 1814
PspPPI RGGWCCY 3 cut(s) 538, 860, 1114
PstI CTGCAG 1 cut(s) 2255
PstNI CAGNNNCTG 1 cut(s) 2327
RseI CAYNNNNRTG 2 cut(s) 1766, 1806
SacI GAGCTC 2 cut(s) 1512, 1936
SaqAI TTAA 9 cut(s) 65, 243, 819, 1299, 2163, 2262, 2286, 2436, 2602
SatI GCNGC 9 cut(s) 114, 197, 741, 948, 1257, 2254, 2308, 2589, 2596
Sau96I GGNCC 7 cut(s) 209, 482, 538, 653, 860, 1114, 1814
ScaI AGTACT 1 cut(s) 2291
SchI GAGTC 2 cut(s) 1135, 1767
SduI GDGCHC 6 cut(s) 27, 1000, 1512, 1803, 1936, 1978
SfaNI GCATC 5 cut(s) 214, 790, 1353, 1993, 2091
SfcI CTRYAG 2 cut(s) 162, 2251
SfuI TTCGAA 1 cut(s) 2035
SinI GGWCC 6 cut(s) 209, 482, 538, 653, 860, 1114
SmiMI CAYNNNNRTG 2 cut(s) 1766, 1806
SmlI CTYRAG 1 cut(s) 1133
SmoI CTYRAG 1 cut(s) 1133
SsiI CCGC 4 cut(s) 135, 1866, 2307, 2399
SspI AATATT 1 cut(s) 766
SspMI CTAG 6 cut(s) 1425, 1557, 1623, 1733, 1835, 2576
SstI GAGCTC 2 cut(s) 1512, 1936
StyI CCWWGG 6 cut(s) 508, 776, 987, 1021, 1284, 1834
TaaI ACNGT 9 cut(s) 125, 209, 1297, 1366, 1471, 1682, 2233, 2363, 2512
TaiI ACGT 2 cut(s) 221, 292
TatI WGTACW 5 cut(s) 178, 684, 1917, 2289, 2507
TauI GCSGC 1 cut(s) 2310
TfiI GAWTC 2 cut(s) 352, 546
Tru1I TTAA 9 cut(s) 65, 243, 819, 1299, 2163, 2262, 2286, 2436, 2602
Tru9I TTAA 9 cut(s) 65, 243, 819, 1299, 2163, 2262, 2286, 2436, 2602
TscAI CASTG 3 cut(s) 740, 1927, 2389
TseFI GTSAC 5 cut(s) 80, 137, 214, 926, 1127
TseI GCWGC 8 cut(s) 113, 196, 740, 947, 1256, 2253, 2588, 2595
Tsp45I GTSAC 5 cut(s) 80, 137, 214, 926, 1127
TspGWI ACGGA 3 cut(s) 156, 846, 1563
TspRI CASTG 3 cut(s) 740, 1927, 2389
Van91I CCANNNNNTGG 3 cut(s) 101, 1204, 1784
VpaK11BI GGWCC 6 cut(s) 209, 482, 538, 653, 860, 1114
VspI ATTAAT 3 cut(s) 65, 243, 819
XagI CCTNNNNNAGG 1 cut(s) 390
XapI RAATTY 4 cut(s) 1033, 1617, 2173, 2393
XceI RCATGY 1 cut(s) 2285
XmaJI CCTAGG 1 cut(s) 1834
XmnI GAANNNNTTC 1 cut(s) 2500
XspI CTAG 6 cut(s) 1425, 1557, 1623, 1733, 1835, 2576
ZrmI AGTACT 1 cut(s) 2291
Zsp2I ATGCAT 1 cut(s) 1546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.