Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0002763

Physical Location & Seq

Genomic Coordinates Forward (+)

1127252 .. 1129780

Gene Structure 2529 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1010100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2529 bp

ATGGCAGTACCAATGAGTCGAGTGCTCTTGATTTCTCTTGTTTTCGTAGTGATCACAGCCGCGCTAGTCCATGCCAAAGGCCACGGACCTCATCATCATCATCATAATCAATCATTGCAGGTTGGCCCCGTGACATATGATGGCAGGTCACTGATTATCAATGGGAAACGCGAGCTTCTGTTTTCGGGTTCAATCCACTACACGCGAAGCACGCCAGAAATGTGGCCTGATATTCTCGAGAAGGCCAAGCGCGGAGGCTTGAATGTGATTCAAACTTATGTGTTTTGGAACATTCATGAGCCTGTGCAAGGGCAATACAACTTTGAAGGAAACTATGATTTGGTGAAGTTCATCAAGCTGGTTCAGGAGCATGGAATGTATGTTACCATTAGGCTTGGTCCATTCATCCAAGCCGAATGGAACCACGGAGGACTTCCTTATTGGCTAAGGGAGGTCCCTGGAATCATATTCCGTTCTGATAACACAGCATACAAGTATCACATGAAGAATTTTGTGGATATGATTGTGAACAAGTTGAGAGATGCGAAGCTGTTTGCTCCACAGGGAGGTCCGATTGTCTTGGCGCAGATTGAAAATGAGTACAATATGGTCCAGCTTGCATATAGAGAGTTGGGAAACAGTTACGTGAAGTGGGCAGCAGAGATTGCGGTGAATCAGAACATCGGAGTGCCATGGATCATGTGCAAGCAGAAGGATGCTCCTGATCCAGTTATTAATGCTTGCAATGGAAGGCATTGCGGAGATACATTCACAGGCCCTAACAAACCCAACAAGCCATCTCTATGGACTGAGAATTGGACTGCTCAATACAGAGTATTTGGTGACCCTCCTTCACAAAGATCAGCAGAGGATATTGCATTTTCCGTTGCTCGCTTTTTCTCAAAGAACGGAGCTCTAACCAACTACTATATGTACCATGGTGGCACAAATTTTGGAAGAACAAGTTCTATTTTCACAACCACCCGTTACTATGATGAAGCTCCTCTCGATGAATATGGTTTGCCAAGGGACCCCAAGTGGAGTCACCTCAAGGACTTGCACAAGGCCTTGAAGTTGTCCAGGAAGGCTTTGCTCTGGGGAGTTCCGGGAGTTCAAAAGATAAGCAAAGATACAGAGGTTCATTTCTATGAGACGGCCGCAACCAACATCTGTGCTGCTTTCTTGACAAACAATAACTTCCAAACTGAAGCAACTGTTAATTGGAGGGGCGTGGATTATTACCTGCCACACCGTTCCATTAGCATCCTCCCCGACTGCAAGACCGTGGTCTTTAACACTCAGAGAGTTGTTTCGCAACTGAACTCTAGGAACTTTGTTCGATCAAAAGTTGCTAATAATCTCAAATGGACCAAGTATGTAGAACCAGTTCCTAGCACCCTTCAAGTTCCAGTCAATAACCCGACCCCGCTGGAGCTGTATAGCTTGTTAAAAGATACCACAGACTACGCATGGTACACCACTAAGCTTGCACTGAGTCCACAAGACTTGCCCATGAAGGAAAGTATTCGACCAGTTCTTCGAATCGCAAGTCTTGGTCACGCATTGCACGCATTCGTCAATGGGGAATACATTGGTTTCGCACACGGGAGCCACGATGAGAAGAGCTTTGTTTTCGAGAAACCAGTTCCCTTGAAGGCTGGAGTCAATCAGATTTCTCTGCTTGCGATGACAATAGGACTTCCAGATAGCGGAGCCTACATGGAGCACAGATATGCAGGGCCTAATCTCGTAGCAGTACTAGGACTGAACACCGGAACACTGGACCTCACGCGAAACGGATGGGGGCATCAGCTTGGTCTGAGCGGAGAAAAGCTTCAAGTGTTCACTGAGGAGGGATCGAAACAAGTTCAATGGCATAAAACAAAGGGAGCAGCAGAGGGTCTCACATGGTACAGGACTTCTTTTGATGCTCCAGAAGGCCATGACCCTGTTGCTATACGAATGGCTGGAATGGGAAAGGGAATGATTTGGGTGAATGGTAAAAGCATCGGCCGCCACTGGACGTCCTTCCTCTCTCCTCTTGGAGAACCAACTCAAGCCGAGTACCACATCCCAAGGTCCTTCATTAAACCTAAACAAAATCTCCTTGTTGTGTTGGAGGAGCAGCCAGCCAAGCCCAAACCCATCGAGATCCTAACCGTCAACAGAGATACTATCTGTAGCTTCATTACTGAATATCATCCTCCGAATGTGAATTCATGGGAAAGGAAGGACAGCGTTTTCCAACCTGTGTTCGATGTTGTCAAATCATCTGCAGACATCAAGTGTCCAAAGAACAAGAAGGTTGTTGCTGTTGAGTTTGCAAGCTTCGGAGATCCAACTGGCATCTGTGGCAGCCTTGTCTTGGGAAAATGCAACTCCCCTGTATCGAAGGAGGTTGTTGAACGACATTGCTTGGGAAAAACTAGCTGCTCGGTCCCGATTGATCGCAATCTTTTCGCCAGCAAAACCGATGGTTGCCCTGATATCAAGAAGACACTTGCTATCCAAGTCAAGTGTGCTATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BetaGal_dom4_5 Gal_Lectin Glyco_hydro_35

Protein Analysis

843

Amino Acids

94.44

Weight (kDa)

9.02

Isoelectric Point (pI)

36.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	50 - 356	1.3e-117	Glycosyl hydrolases family 35
Glyco_hydro_42	PF02449	72 - 203	7.9e-07	Beta-galactosidase
GHD	PF17834	381 - 436	2.3e-20	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	635 - 701	2.1e-23	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	761 - 840	2.2e-16	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002763)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16730 AT4G35010
fragaria_vesca	FvH4_2g01270 FvH4_4g13900
malus_domestica	MD04G1010100.v1.1 MD05G1121000.v1.1 MD10G1095200.v1.1 MD13G1284600.v1.1
prunus_persica	Prupe.1G009200_v2.0.a1 Prupe.1G009300_v2.0.a1 Prupe.8G164800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0475241 RchiOBHm_Chr4g0415021 RchiOBHm_Chr6g0251931
rosa_laevigata	RLG00000008102 RLG00000015418 RLG00000023857
rosa_multiflora	Rmu_sc0003611.1_g000013 Rmu_sc0008157.1_g000007 Rmu_ssc0000303.1_g000015
rosa_roxburghii	Rroxscaffold_5G00358760 Rroxscaffold_6G00406440
rosa_rugosa	Rorug03G0147300 Rorug04G0131700 Rorug05G0505100 Rorug05G0505200 Rorug05G0505200
rosa_samantha	Rh3AG197300 Rh4AG191000 Rh4BG189100 Rh4CG202000 Rh4DG189500 Rh6AG016400 Rh6BG014400 Rh6CG011300 Rh6DG012600
rosa_wichuraiana	Rw3G017940 Rw4G016250 Rw6G001570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	2027
Acc36I	ACCTGC	3 cut(s)	109, 135, 1251
AccBSI	CCGCTC	1 cut(s)	1824
AccII	CGCG	5 cut(s)	62, 171, 205, 252, 1792
AciI	CCGC	9 cut(s)	60, 252, 668, 759, 1158, 1427, 1710, 1824, 2014
AclWI	GGATC	5 cut(s)	704, 719, 1864, 2146, 2330
AcoI	YGGCCR	2 cut(s)	1155, 2011
AcsI	RAATTY	3 cut(s)	508, 949, 2215
AcuI	CTGAAG	1 cut(s)	1227
AcyI	GRCGYC	1 cut(s)	2024
AfaI	GTAC	7 cut(s)	9, 602, 935, 1475, 1758, 1913, 2066
AfiI	CCNNNNNNNGG	4 cut(s)	308, 566, 1709, 2365
AjnI	CCWGG	2 cut(s)	457, 1079
Alw21I	GWGCWC	3 cut(s)	27, 916, 1728
Alw26I	GTCTC	2 cut(s)	1145, 1907
AlwI	GGATC	5 cut(s)	704, 719, 1864, 2146, 2330
Ama87I	CYCGRG	1 cut(s)	236
ApeKI	GCWGC	6 cut(s)	656, 1175, 1892, 2125, 2355, 2430
ApoI	RAATTY	3 cut(s)	508, 949, 2215
ArsI	GACNNNNNNTTYG	4 cut(s)	1521, 1534, 1553, 1566
AseI	ATTAAT	1 cut(s)	735
Asp700I	GAANNNNTTC	4 cut(s)	964, 1386, 1524, 1833
AspLEI	GCGC	3 cut(s)	64, 252, 586
AsuC2I	CCSGG	1 cut(s)	1107
AsuHPI	GGTGA	5 cut(s)	355, 682, 854, 1037, 2005
AsuII	TTCGAA	1 cut(s)	1540
AvaI	CYCGRG	1 cut(s)	236
BaeI	ACNNNNGTAYC	2 cut(s)	1122, 1155
BanII	GRGCYC	1 cut(s)	916
BbsI	GAAGAC	1 cut(s)	2501
Bbv12I	GWGCWC	3 cut(s)	27, 916, 1728
BbvI	GCAGC	6 cut(s)	668, 1162, 1904, 2137, 2367, 2417
BccI	CCATC	5 cut(s)	134, 805, 1794, 2153, 2468
BceAI	ACGGC	1 cut(s)	1170
BcgI	CGANNNNNNTGC	2 cut(s)	2440, 2474
BciT130I	CCWGG	2 cut(s)	459, 1081
BclI	TGATCA	1 cut(s)	51
BcnI	CCSGG	1 cut(s)	1107
BcoDI	GTCTC	2 cut(s)	1145, 1907
BfaI	CTAG	5 cut(s)	65, 1326, 1392, 1760, 2427
BfmI	CTRYAG	2 cut(s)	2179, 2274
BfuAI	ACCTGC	3 cut(s)	109, 135, 1251
BisI	GCNGC	9 cut(s)	60, 657, 1158, 1176, 1893, 2014, 2126, 2356, 2431
BlsI	GCNGC	9 cut(s)	61, 658, 1159, 1177, 1894, 2015, 2127, 2357, 2432
BmcAI	AGTACT	1 cut(s)	1758
Bme1390I	CCNGG	3 cut(s)	459, 1081, 1107
BmeT110I	CYCGRG	1 cut(s)	236
BmiI	GGNNCC	8 cut(s)	127, 422, 456, 1031, 1032, 1610, 1714, 2439
BmrFI	CCNGG	3 cut(s)	459, 1081, 1107
BmsI	GCATC	7 cut(s)	532, 706, 1272, 1816, 1918, 2016, 2355
BoxI	GACNNNNGTC	1 cut(s)	1286
BpiI	GAAGAC	1 cut(s)	2501
BpmI	CTGGAG	3 cut(s)	1451, 1680, 1917
Bpu10I	CCTNAGC	1 cut(s)	446
Bpu14I	TTCGAA	1 cut(s)	1540
BpuEI	CTTGAG	2 cut(s)	1034, 2040
BpuMI	CCSGG	1 cut(s)	1107
BsaAI	YACGTR	1 cut(s)	646
BsaBI	GATNNNNATC	1 cut(s)	2451
BsaHI	GRCGYC	1 cut(s)	2024
BsaI	GGTCTC	1 cut(s)	1907
BsaJI	CCNNGG	8 cut(s)	82, 424, 457, 692, 937, 1025, 1284, 2075
BsaWI	WCCGGW	1 cut(s)	1772
BsaXI	ACNNNNNCTCC	2 cut(s)	2088, 2118
Bsc4I	CCNNNNNNNGG	4 cut(s)	308, 566, 1709, 2365
Bse1I	ACTGG	8 cut(s)	728, 1385, 1409, 1532, 1643, 1785, 2024, 2347
Bse3DI	GCAATG	5 cut(s)	113, 751, 754, 1562, 2410
Bse8I	GATNNNNATC	1 cut(s)	2451
BseBI	CCWGG	2 cut(s)	459, 1081
BseDI	CCNNGG	8 cut(s)	82, 424, 457, 692, 937, 1025, 1284, 2075
BseGI	GGATG	6 cut(s)	405, 721, 1263, 1805, 2070, 2200
BseJI	GATNNNNATC	1 cut(s)	2451
BseLI	CCNNNNNNNGG	4 cut(s)	308, 566, 1709, 2365
BseMI	GCAATG	5 cut(s)	113, 751, 754, 1562, 2410
BseMII	CTCAG	5 cut(s)	801, 1313, 1484, 1811, 1839
BseNI	ACTGG	8 cut(s)	728, 1385, 1409, 1532, 1643, 1785, 2024, 2347
BseRI	GAGGAG	4 cut(s)	993, 1865, 2028, 2135
BseX3I	CGGCCG	2 cut(s)	1155, 2011
BseXI	GCAGC	6 cut(s)	668, 1162, 1904, 2137, 2367, 2417
Bsh1236I	CGCG	5 cut(s)	62, 171, 205, 252, 1792
Bsh1285I	CGRYCG	2 cut(s)	1158, 2014
BsiEI	CGRYCG	2 cut(s)	1158, 2014
BsiHKAI	GWGCWC	3 cut(s)	27, 916, 1728
BsiHKCI	CYCGRG	1 cut(s)	236
BsiSI	CCGG	2 cut(s)	1106, 1773
BslFI	GGGAC	3 cut(s)	440, 1043, 2423
BslI	CCNNNNNNNGG	4 cut(s)	308, 566, 1709, 2365
BsmAI	GTCTC	2 cut(s)	1145, 1907
BsmBI	CGTCTC	1 cut(s)	1145
BsmFI	GGGAC	3 cut(s)	440, 1043, 2423
BsmI	GAATGC	1 cut(s)	1571
Bso31I	GGTCTC	1 cut(s)	1907
BsoBI	CYCGRG	1 cut(s)	236
Bsp119I	TTCGAA	1 cut(s)	1540
Bsp1286I	GDGCHC	3 cut(s)	27, 916, 1728
Bsp143I	GATC	9 cut(s)	51, 696, 724, 860, 1340, 1856, 2151, 2335, 2446
Bsp19I	CCATGG	2 cut(s)	692, 937
BspACI	CCGC	9 cut(s)	60, 252, 668, 759, 1158, 1427, 1710, 1824, 2014
BspCNI	CTCAG	5 cut(s)	802, 1312, 1485, 1812, 1840
BspFNI	CGCG	5 cut(s)	62, 171, 205, 252, 1792
BspHI	TCATGA	1 cut(s)	295
BspLI	GGNNCC	8 cut(s)	127, 422, 456, 1031, 1032, 1610, 1714, 2439
BspMAI	CTGCAG	1 cut(s)	2278
BspMI	ACCTGC	3 cut(s)	109, 135, 1251
BspPI	GGATC	5 cut(s)	704, 719, 1864, 2146, 2330
BspQI	GCTCTTC	1 cut(s)	1616
BspT104I	TTCGAA	1 cut(s)	1540
BspTNI	GGTCTC	1 cut(s)	1907
BsrBI	CCGCTC	1 cut(s)	1824
BsrDI	GCAATG	5 cut(s)	113, 751, 754, 1562, 2410
BsrI	ACTGG	8 cut(s)	728, 1385, 1409, 1532, 1643, 1785, 2024, 2347
BssECI	CCNNGG	8 cut(s)	82, 424, 457, 692, 937, 1025, 1284, 2075
BssMI	GATC	9 cut(s)	51, 696, 724, 860, 1340, 1856, 2151, 2335, 2446
BssNI	GRCGYC	1 cut(s)	2024
BssT1I	CCWWGG	4 cut(s)	692, 937, 1025, 2075
Bst2UI	CCWGG	2 cut(s)	459, 1081
Bst4CI	ACNGT	5 cut(s)	641, 1216, 1253, 1285, 2161
Bst6I	CTCTTC	1 cut(s)	1616
BstACI	GRCGYC	1 cut(s)	2024
BstAPI	GCANNNNNTGC	1 cut(s)	665
BstBAI	YACGTR	1 cut(s)	646
BstBI	TTCGAA	1 cut(s)	1540
BstDEI	CTNAG	7 cut(s)	446, 810, 1299, 1482, 1493, 1820, 1848
BstDSI	CCRYGG	5 cut(s)	82, 424, 692, 937, 1284
BstEII	GGTNACC	1 cut(s)	842
BstENI	CCTNNNNNAGG	1 cut(s)	306
BstF5I	GGATG	6 cut(s)	405, 721, 1263, 1805, 2070, 2200
BstFNI	CGCG	5 cut(s)	62, 171, 205, 252, 1792
BstHHI	GCGC	3 cut(s)	64, 252, 586
BstKTI	GATC	9 cut(s)	54, 699, 727, 863, 1343, 1859, 2154, 2338, 2449
BstMAI	GTCTC	2 cut(s)	1145, 1907
BstMBI	GATC	9 cut(s)	51, 696, 724, 860, 1340, 1856, 2151, 2335, 2446
BstMCI	CGRYCG	2 cut(s)	1158, 2014
BstMWI	GCNNNNNNNGC	6 cut(s)	211, 665, 1568, 2013, 2134, 2352
BstNI	CCWGG	2 cut(s)	459, 1081
BstPAI	GACNNNNGTC	1 cut(s)	1286
BstPI	GGTNACC	1 cut(s)	842
BstSCI	CCNGG	3 cut(s)	457, 1079, 1105
BstSFI	CTRYAG	2 cut(s)	2179, 2274
BstUI	CGCG	5 cut(s)	62, 171, 205, 252, 1792
BstV1I	GCAGC	6 cut(s)	668, 1162, 1904, 2137, 2367, 2417
BstV2I	GAAGAC	1 cut(s)	2501
BstX2I	RGATCY	2 cut(s)	2151, 2335
BstXI	CCANNNNNNTGG	2 cut(s)	222, 804
BstYI	RGATCY	2 cut(s)	2151, 2335
BstZI	CGGCCG	2 cut(s)	1155, 2011
BtgI	CCRYGG	5 cut(s)	82, 424, 692, 937, 1284
BtgZI	GCGATG	1 cut(s)	1700
BtsCI	GGATG	6 cut(s)	405, 721, 1263, 1805, 2070, 2200
BtsIMutI	CAGTG	5 cut(s)	149, 1490, 1778, 1845, 2017
BveI	ACCTGC	3 cut(s)	109, 135, 1251
CciI	TCATGA	1 cut(s)	295
CfoI	GCGC	3 cut(s)	64, 252, 586
Csp6I	GTAC	7 cut(s)	8, 601, 934, 1474, 1757, 1912, 2065
CspCI	CAANNNNNGTGG	4 cut(s)	1468, 1488, 1503, 1523
CviQI	GTAC	7 cut(s)	8, 601, 934, 1474, 1757, 1912, 2065
DdeI	CTNAG	7 cut(s)	446, 810, 1299, 1482, 1493, 1820, 1848
DpnI	GATC	9 cut(s)	53, 698, 726, 862, 1342, 1858, 2153, 2337, 2448
DpnII	GATC	9 cut(s)	51, 696, 724, 860, 1340, 1856, 2151, 2335, 2446
EaeI	YGGCCR	2 cut(s)	1155, 2011
EagI	CGGCCG	2 cut(s)	1155, 2011
Eam1104I	CTCTTC	1 cut(s)	1616
EarI	CTCTTC	1 cut(s)	1616
Ecl136II	GAGCTC	1 cut(s)	914
EclXI	CGGCCG	2 cut(s)	1155, 2011
Eco130I	CCWWGG	4 cut(s)	692, 937, 1025, 2075
Eco147I	AGGCCT	1 cut(s)	1067
Eco24I	GRGCYC	1 cut(s)	916
Eco31I	GGTCTC	1 cut(s)	1907
Eco32I	GATATC	1 cut(s)	2488
Eco52I	CGGCCG	2 cut(s)	1155, 2011
Eco53kI	GAGCTC	1 cut(s)	914
Eco57I	CTGAAG	1 cut(s)	1227
Eco88I	CYCGRG	1 cut(s)	236
Eco91I	GGTNACC	1 cut(s)	842
EcoICRI	GAGCTC	1 cut(s)	914
EcoNI	CCTNNNNNAGG	1 cut(s)	306
EcoO109I	RGGNCCY	5 cut(s)	454, 776, 1030, 1739, 2079
EcoO65I	GGTNACC	1 cut(s)	842
EcoRI	GAATTC	1 cut(s)	2215
EcoRII	CCWGG	2 cut(s)	457, 1079
EcoRV	GATATC	1 cut(s)	2488
EcoT14I	CCWWGG	4 cut(s)	692, 937, 1025, 2075
EcoT38I	GRGCYC	1 cut(s)	916
ErhI	CCWWGG	4 cut(s)	692, 937, 1025, 2075
Esp3I	CGTCTC	1 cut(s)	1145
FaqI	GGGAC	3 cut(s)	440, 1043, 2423
FauI	CCCGC	1 cut(s)	1434
FauNDI	CATATG	1 cut(s)	136
FbaI	TGATCA	1 cut(s)	51
Fnu4HI	GCNGC	9 cut(s)	60, 657, 1158, 1176, 1893, 2014, 2126, 2356, 2431
FokI	GGATG	6 cut(s)	392, 728, 1250, 1812, 2057, 2187
FriOI	GRGCYC	1 cut(s)	916
Fsp4HI	GCNGC	9 cut(s)	60, 657, 1158, 1176, 1893, 2014, 2126, 2356, 2431
FspBI	CTAG	5 cut(s)	65, 1326, 1392, 1760, 2427
GlaI	GCGC	3 cut(s)	63, 251, 585
GluI	GCNGC	9 cut(s)	60, 657, 1158, 1176, 1893, 2014, 2126, 2356, 2431
GsuI	CTGGAG	3 cut(s)	1451, 1680, 1917
HapII	CCGG	2 cut(s)	1106, 1773
HhaI	GCGC	3 cut(s)	64, 252, 586
Hin1I	GRCGYC	1 cut(s)	2024
Hin6I	GCGC	3 cut(s)	62, 250, 584
HinP1I	GCGC	3 cut(s)	62, 250, 584
HincII	GTYRAC	1 cut(s)	2164
HindII	GTYRAC	1 cut(s)	2164
HindIII	AAGCTT	3 cut(s)	1484, 1832, 2326
HinfI	GANTC	8 cut(s)	16, 268, 462, 673, 1042, 1495, 1542, 1662
HpaII	CCGG	2 cut(s)	1106, 1773
HphI	GGTGA	5 cut(s)	355, 682, 854, 1037, 2005
Hpy166II	GTNNAC	5 cut(s)	529, 1476, 1499, 1845, 2164
Hpy188I	TCNGA	9 cut(s)	478, 573, 678, 686, 1302, 1671, 1821, 2208, 2333
Hpy8I	GTNNAC	5 cut(s)	529, 1476, 1499, 1845, 2164
HpyCH4III	ACNGT	5 cut(s)	641, 1216, 1253, 1285, 2161
HpyCH4IV	ACGT	2 cut(s)	645, 2024
HpyF10VI	GCNNNNNNNGC	6 cut(s)	211, 665, 1568, 2013, 2134, 2352
HpyF3I	CTNAG	7 cut(s)	446, 810, 1299, 1482, 1493, 1820, 1848
HpySE526I	ACGT	2 cut(s)	645, 2024
Hsp92I	GRCGYC	1 cut(s)	2024
HspAI	GCGC	3 cut(s)	62, 250, 584
KflI	GGGWCCC	1 cut(s)	1030
Ksp22I	TGATCA	1 cut(s)	51
Kzo9I	GATC	9 cut(s)	51, 696, 724, 860, 1340, 1856, 2151, 2335, 2446
LguI	GCTCTTC	1 cut(s)	1616
Lsp1109I	GCAGC	6 cut(s)	668, 1162, 1904, 2137, 2367, 2417
LweI	GCATC	7 cut(s)	532, 706, 1272, 1816, 1918, 2016, 2355
MaeI	CTAG	5 cut(s)	65, 1326, 1392, 1760, 2427
MaeII	ACGT	2 cut(s)	645, 2024
MaeIII	GTNAC	8 cut(s)	130, 147, 382, 641, 842, 986, 1043, 1556
MalI	GATC	9 cut(s)	53, 698, 726, 862, 1342, 1858, 2153, 2337, 2448
MbiI	CCGCTC	1 cut(s)	1824
MboI	GATC	9 cut(s)	51, 696, 724, 860, 1340, 1856, 2151, 2335, 2446
MboII	GAAGA	5 cut(s)	517, 969, 1529, 1633, 2506
MflI	RGATCY	2 cut(s)	2151, 2335
MhlI	GDGCHC	3 cut(s)	27, 916, 1728
MluCI	AATT	5 cut(s)	508, 814, 949, 1219, 2215
MlyI	GAGTC	4 cut(s)	25, 1051, 1504, 1671
MmeI	TCCRAC	3 cut(s)	2097, 2269, 2363
MroXI	GAANNNNTTC	4 cut(s)	964, 1386, 1524, 1833
MseI	TTAA	5 cut(s)	735, 1218, 1293, 1448, 2088
MslI	CAYNNNNRTG	4 cut(s)	686, 802, 1146, 1731
MspA1I	CMGCKG	1 cut(s)	1429
MspI	CCGG	2 cut(s)	1106, 1773
MspR9I	CCNGG	3 cut(s)	459, 1081, 1107
Mva1269I	GAATGC	1 cut(s)	1571
MvaI	CCWGG	2 cut(s)	459, 1081
MvnI	CGCG	5 cut(s)	62, 171, 205, 252, 1792
MwoI	GCNNNNNNNGC	6 cut(s)	211, 665, 1568, 2013, 2134, 2352
NciI	CCSGG	1 cut(s)	1107
NcoI	CCATGG	2 cut(s)	692, 937
NdeI	CATATG	1 cut(s)	136
NdeII	GATC	9 cut(s)	51, 696, 724, 860, 1340, 1856, 2151, 2335, 2446
NlaIV	GGNNCC	8 cut(s)	127, 422, 456, 1031, 1032, 1610, 1714, 2439
NmeAIII	GCCGAG	1 cut(s)	2086
NmuCI	GTSAC	5 cut(s)	130, 147, 842, 1043, 1556
NspV	TTCGAA	1 cut(s)	1540
PaeR7I	CTCGAG	1 cut(s)	236
PagI	TCATGA	1 cut(s)	295
PceI	AGGCCT	1 cut(s)	1067
PciSI	GCTCTTC	1 cut(s)	1616
PcsI	WCGNNNNNNNCGW	1 cut(s)	1611
PctI	GAATGC	1 cut(s)	1571
PdmI	GAANNNNTTC	4 cut(s)	964, 1386, 1524, 1833
PfeI	GAWTC	4 cut(s)	268, 462, 673, 1542
PfoI	TCCNGGA	2 cut(s)	1079, 1105
PkrI	GCNGC	9 cut(s)	61, 658, 1159, 1177, 1894, 2015, 2127, 2357, 2432
PleI	GAGTC	4 cut(s)	24, 1050, 1503, 1670
PpsI	GAGTC	4 cut(s)	24, 1050, 1503, 1670
Ppu21I	YACGTR	1 cut(s)	646
PpuMI	RGGWCCY	3 cut(s)	454, 1030, 2079
PshAI	GACNNNNGTC	1 cut(s)	1286
PshBI	ATTAAT	1 cut(s)	735
Psp124BI	GAGCTC	1 cut(s)	916
Psp5II	RGGWCCY	3 cut(s)	454, 1030, 2079
Psp6I	CCWGG	2 cut(s)	457, 1079
PspEI	GGTNACC	1 cut(s)	842
PspGI	CCWGG	2 cut(s)	457, 1079
PspN4I	GGNNCC	8 cut(s)	127, 422, 456, 1031, 1032, 1610, 1714, 2439
PspPPI	RGGWCCY	3 cut(s)	454, 1030, 2079
PsrI	GAACNNNNNNTAC	2 cut(s)	1371, 1403
PstI	CTGCAG	1 cut(s)	2278
PsuI	RGATCY	2 cut(s)	2151, 2335
RsaI	GTAC	7 cut(s)	9, 602, 935, 1475, 1758, 1913, 2066
RsaNI	GTAC	7 cut(s)	8, 601, 934, 1474, 1757, 1912, 2065
RseI	CAYNNNNRTG	4 cut(s)	686, 802, 1146, 1731
SacI	GAGCTC	1 cut(s)	916
SapI	GCTCTTC	1 cut(s)	1616
SaqAI	TTAA	5 cut(s)	735, 1218, 1293, 1448, 2088
SatI	GCNGC	9 cut(s)	60, 657, 1158, 1176, 1893, 2014, 2126, 2356, 2431
Sau3AI	GATC	9 cut(s)	51, 696, 724, 860, 1340, 1856, 2151, 2335, 2446
ScaI	AGTACT	1 cut(s)	1758
SchI	GAGTC	4 cut(s)	25, 1051, 1504, 1671
ScrFI	CCNGG	3 cut(s)	459, 1081, 1107
SduI	GDGCHC	3 cut(s)	27, 916, 1728
SfaNI	GCATC	7 cut(s)	532, 706, 1272, 1816, 1918, 2016, 2355
SfcI	CTRYAG	2 cut(s)	2179, 2274
Sfr274I	CTCGAG	1 cut(s)	236
SfuI	TTCGAA	1 cut(s)	1540
SlaI	CTCGAG	1 cut(s)	236
SmiMI	CAYNNNNRTG	4 cut(s)	686, 802, 1146, 1731
SmlI	CTYRAG	3 cut(s)	236, 1049, 2055
SmoI	CTYRAG	3 cut(s)	236, 1049, 2055
Sse9I	AATT	5 cut(s)	508, 814, 949, 1219, 2215
SseBI	AGGCCT	1 cut(s)	1067
SsiI	CCGC	9 cut(s)	60, 252, 668, 759, 1158, 1427, 1710, 1824, 2014
SspMI	CTAG	5 cut(s)	65, 1326, 1392, 1760, 2427
SstI	GAGCTC	1 cut(s)	916
StuI	AGGCCT	1 cut(s)	1067
StyD4I	CCNGG	3 cut(s)	457, 1079, 1105
StyI	CCWWGG	4 cut(s)	692, 937, 1025, 2075
TaaI	ACNGT	5 cut(s)	641, 1216, 1253, 1285, 2161
TaiI	ACGT	2 cut(s)	648, 2027
TaqII	GACCGA	1 cut(s)	2425
TasI	AATT	5 cut(s)	508, 814, 949, 1219, 2215
TatI	WGTACW	2 cut(s)	600, 1756
TauI	GCSGC	3 cut(s)	62, 1160, 2016
TfiI	GAWTC	4 cut(s)	268, 462, 673, 1542
Tru1I	TTAA	5 cut(s)	735, 1218, 1293, 1448, 2088
Tru9I	TTAA	5 cut(s)	735, 1218, 1293, 1448, 2088
TscAI	CASTG	5 cut(s)	156, 1497, 1785, 1852, 2024
TseFI	GTSAC	5 cut(s)	130, 147, 842, 1043, 1556
TseI	GCWGC	6 cut(s)	656, 1175, 1892, 2125, 2355, 2430
Tsp45I	GTSAC	5 cut(s)	130, 147, 842, 1043, 1556
TspGWI	ACGGA	6 cut(s)	99, 441, 461, 874, 924, 1812
TspRI	CASTG	5 cut(s)	156, 1497, 1785, 1852, 2024
VspI	ATTAAT	1 cut(s)	735
XagI	CCTNNNNNAGG	1 cut(s)	306
XapI	RAATTY	3 cut(s)	508, 949, 2215
XhoI	CTCGAG	1 cut(s)	236
XmnI	GAANNNNTTC	4 cut(s)	964, 1386, 1524, 1833
XspI	CTAG	5 cut(s)	65, 1326, 1392, 1760, 2427
ZraI	GACGTC	1 cut(s)	2025
ZrmI	AGTACT	1 cut(s)	1758

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.