Rh6AG016400

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0002763
Physical Location & Seq
Forward (+)
1651733 .. 1657504
5772 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG016400.1

Sequence Viewer

Length: 2322 bp
ATGTTTTTTGTTGCCTTTCAGATGTGGCCTGATATTCTGAAGAAGGCTAAACAAGGAGGTTTGAATGTGATTCAAACTTATGTGTTTTGGAACATTCATGAGCCTGTGCAAGGCAAGTGGGAGTTCGAGGGAAACTATGATTTGGTGAAGTTTATTAGGTTGATCCAGGAGAATGGAATGTATGCTACCCTCAGGGTTGGACCATTCATCCAAGCAGAATGGAACCATGGAGGTCTTCCATATTGGCTGAGAGAGGTCCCAGGAATCACCTTCCGATCAGATAATGAACCATACAAGAAACACATGGAGCAATATGTGAACAAGATAATGAAGATGATGAAGGATGAAAAGCTATTTGCTCCACAAGGAGGTCCAATCATCTTAGCTCAGATTGAGAATGAGTACAATCAAATCCAACTTGCATATAGAGAGTTGGGAGATACATATGTGCAGTGGGCAGCAAAGCTGGCAGTTGGGCAGAATATTGGAGTGCCATGGATCATGTGCAAGCAAAAGGATGCTCCTGATCCAGTGATTAATACTTGCAACGGAAGGCATTGTGGAGATACTTTTTCAGGACCTAACAAACCCAACAAGCCTGCTCTATGGACTGAGAATTGGACAGCTCAGTACCGAGTATTTGGTGACCCTCCTTCACAAAGAGCAGCAGAAGACCTTGCATTTTCAGTTGCTCGGTTTTTCTCCAAGGGTGGGGCTCTAACCAACTACTATATGTACCATGGTGGAACGAATTTTGGAAGAACAAGTGCTGTTTTCACAACAACTCGTTACTACGATGAGGCTCCTCTCGATGAATATGCTTTGCAAAGGGACCCGAAATGGAGTCACCTCAAGGACTTGCACAAGGCTATAAATCTATGCAAGAAAACTTTGCTTACTGGAACTCCCGGAGACCAAAGGTTGGGGGAAGAAACTGAGGTTCGTTTCTATGAGAAGCCAGGGACAGAGTGTGCTGCTTTCATAGCAAATAATCACTCGACCATGGAAGCAACCGTTAATTGGAGGGGACAGAAGTTTCTCCTGCCACCAGCTTCCATTAGCATCCTCCCCGACTGCAAGACTGTGGTCTTCAATACTCAACAAATTGTATCCCAACATAATTCAAGGAACTTTGTTACAGCTAGTAAAAAAATGAACTGGGAAATGTCTCCCGAACCCATTGCAACGGTCGCGCAAATCCCATCCAATAGTAGGGAAATGCAGGAGCTCTATGGCTTGCTAAAAGATACCACAGACTATGCATGGTACACAACTAGCATTGACCTAAGTCCGTATGACTTGCCGATGAAGGAGACTATCAGGCCAGTTGTACGAATTCCTAGTCTTGGTCATGCAATGGCTGTATTTGTCAATGATGAATATATTGGATCGCAACACGGTAGCCACGCAGAGAAAGGTTTCGTGTTCGAGGCACCTGCTAAAATGAAGCTAGGGGCGAACCATATCTCTCTGCTTTGCATGGTAGTGGGACTCCCAGATAGTGGAGCGTACATGGAGCACAGGTATGCAGGGCCTCGTTCTGTAACAATCCTAGGATTGAACACCGGAACAATCGACCTGTCCGCAAATGGCTGGGGACATAGGGCTGGCCTGAATGGTGAAAAGCTCGAAGTGTACACTGAGAAGGGAGCTCAGAAAGTGAAATGGACTAAGGCAACAGGTAAAGGAAGGGCTCTCACATGGTACAAGACAAGTTTTGATGCTCCTTCAGGAAGAGACCCTTGTGCTATTCGAATGACCGGTATGGGCAAGGGAATGGTATGGGTGAATGGTATAAGCATCGGCCGTCATTGGATGTCCTTCCTCTCTCCTCTTGGACAACCTACTCAAATCGAGTACCACATCCCAAGAGCCTTCATTAAGCCTAAGAATTTCCTTGTTGTATTGGAGGAGGAGCCAGCAGACCCAAAAGACATTGAGATCGTAACAGTCAACAGAGACACCATCTGCAGCCTTATTAAACAGGACTATCCTGGACATGTTAAGTACTGGGATAGAAAAGGCGGCAAGCTCACAACAGCTCCTGGAAAAAGTCTCCAACCATCAGCTCTCCTCAACTGTCCAAACTACAAAAAGATCAGTGCTATTGAATTCGCGGGCTATGGCAATCCTGACGGCTTCTGTGGAGCCTTTAAATTGGGACAATGCAATGCTCCTGAAGCCAAGAAGGTCGTCGAAGATCATTGCTTGGGAAAGATTTCATGTACAGTCCCAAATGACCCCAGCCTCTTCTTCAAGTCTGGTGATCCTTGCCCTGGTATCAAGAAGACACTAGCTATCCAAGCAGCGTGCAGCTTTTAA

Protein Analysis

773

Amino Acids

86.59

Weight (kDa)

8.67

Isoelectric Point (pI)

35.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 7 - 290 3.6e-105 Glycosyl hydrolases family 35
Glyco_hydro_42 PF02449 7 - 137 9.3e-06 Beta-galactosidase
GHD PF17834 314 - 369 3.7e-20 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 566 - 630 3e-22 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 692 - 771 3e-17 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1444
Acc36I ACCTGC 1 cut(s) 1444
AccB1I GGYRCC 1 cut(s) 1432
AccB7I CCANNNNNTGG 2 cut(s) 922, 1502
AccII CGCG 2 cut(s) 1193, 2117
AciI CCGC 3 cut(s) 1584, 2025, 2117
AclWI GGATC 5 cut(s) 157, 506, 521, 1396, 2261
AcoI YGGCCR 1 cut(s) 1804
AcsI RAATTY 4 cut(s) 751, 1335, 1891, 2111
AcuI CTGAAG 3 cut(s) 59, 1713, 2199
AfiI CCNNNNNNNGG 9 cut(s) 110, 368, 711, 922, 1020, 1212, 1346, 1502, 2276
AflIII ACRYGT 1 cut(s) 1999
AgeI ACCGGT 1 cut(s) 1760
AgsI TTSAA 7 cut(s) 64, 74, 1093, 1125, 1561, 2111, 2257
AjnI CCWGG 6 cut(s) 165, 259, 958, 1993, 2044, 2275
Alw21I GWGCWC 3 cut(s) 1230, 1521, 1654
Alw26I GTCTC 6 cut(s) 906, 1173, 1307, 1731, 1953, 2060
AlwI GGATC 5 cut(s) 157, 506, 521, 1396, 2261
AlwNI CAGNNNCTG 1 cut(s) 2045
AoxI GGCC 5 cut(s) 26, 1322, 1532, 1609, 1804
ApeKI GCWGC 6 cut(s) 458, 665, 974, 1971, 2306, 2313
ApoI RAATTY 4 cut(s) 751, 1335, 1891, 2111
ArsI GACNNNNNNTTYG 2 cut(s) 1327, 1359
AseI ATTAAT 1 cut(s) 537
AsiGI ACCGGT 1 cut(s) 1760
Asp700I GAANNNNTTC 2 cut(s) 1418, 2218
AspA2I CCTAGG 1 cut(s) 1552
AspLEI GCGC 1 cut(s) 1195
AspS9I GGNCC 6 cut(s) 200, 256, 371, 578, 832, 1532
AsuC2I CCSGG 1 cut(s) 909
AsuHPI GGTGA 7 cut(s) 157, 259, 656, 839, 1631, 1798, 2276
AsuII TTCGAA 1 cut(s) 1753
AvaII GGWCC 5 cut(s) 200, 256, 371, 578, 832
AvrII CCTAGG 1 cut(s) 1552
AxyI CCTNAGG 1 cut(s) 191
BaeI ACNNNNGTAYC 2 cut(s) 432, 465
BanI GGYRCC 1 cut(s) 1432
BanII GRGCYC 4 cut(s) 718, 1230, 1654, 1696
BbsI GAAGAC 4 cut(s) 227, 678, 1081, 2294
Bbv12I GWGCWC 3 cut(s) 1230, 1521, 1654
BbvI GCAGC 5 cut(s) 470, 677, 961, 1983, 2318
BccI CCATC 3 cut(s) 1210, 1973, 2071
BceAI ACGGC 2 cut(s) 1791, 2152
BcgI CGANNNNNNTGC 6 cut(s) 800, 834, 1163, 1197, 1418, 1452
BciT130I CCWGG 6 cut(s) 167, 261, 960, 1995, 2046, 2277
BciVI GTATCC 1 cut(s) 1120
BcnI CCSGG 1 cut(s) 909
BcoDI GTCTC 6 cut(s) 906, 1173, 1307, 1731, 1953, 2060
BfaI CTAG 6 cut(s) 1143, 1275, 1341, 1451, 1553, 2294
BfmI CTRYAG 1 cut(s) 1969
BfuAI ACCTGC 1 cut(s) 1444
BfuI GTATCC 1 cut(s) 1120
BisI GCNGC 7 cut(s) 459, 666, 975, 1972, 2026, 2307, 2314
BlnI CCTAGG 1 cut(s) 1552
BlsI GCNGC 7 cut(s) 460, 667, 976, 1973, 2027, 2308, 2315
BmcAI AGTACT 1 cut(s) 2009
Bme1390I CCNGG 7 cut(s) 167, 261, 909, 960, 1995, 2046, 2277
Bme18I GGWCC 5 cut(s) 200, 256, 371, 578, 832
BmgT120I GGNCC 6 cut(s) 200, 256, 371, 578, 832, 1532
BmiI GGNNCC 8 cut(s) 224, 258, 804, 833, 834, 1434, 1917, 2149
BmrFI CCNGG 7 cut(s) 167, 261, 909, 960, 1995, 2046, 2277
BmrI ACTGGG 2 cut(s) 1168, 2020
BmsI GCATC 4 cut(s) 508, 1071, 1711, 1809
BmuI ACTGGG 2 cut(s) 1168, 2020
BoxI GACNNNNGTC 2 cut(s) 1085, 1287
BpiI GAAGAC 4 cut(s) 227, 678, 1081, 2294
Bpu14I TTCGAA 1 cut(s) 1753
BpuEI CTTGAG 1 cut(s) 836
BpuMI CCSGG 1 cut(s) 909
BsaI GGTCTC 2 cut(s) 906, 1731
BsaJI CCNNGG 9 cut(s) 226, 259, 494, 705, 739, 959, 1002, 1552, 2275
BsaWI WCCGGW 2 cut(s) 1565, 1760
BsaXI ACNNNNNCTCC 6 cut(s) 889, 919, 1508, 1538, 2026, 2056
Bsc4I CCNNNNNNNGG 9 cut(s) 110, 368, 711, 922, 1020, 1212, 1346, 1502, 2276
Bse118I RCCGGY 1 cut(s) 1760
Bse1I ACTGG 5 cut(s) 530, 904, 1163, 1325, 2015
Bse21I CCTNAGG 1 cut(s) 191
Bse3DI GCAATG 4 cut(s) 1179, 1362, 2176, 2203
BseBI CCWGG 6 cut(s) 167, 261, 960, 1995, 2046, 2277
BseDI CCNNGG 9 cut(s) 226, 259, 494, 705, 739, 959, 1002, 1552, 2275
BseGI GGATG 7 cut(s) 207, 349, 523, 1062, 1202, 1821, 1863
BseLI CCNNNNNNNGG 9 cut(s) 110, 368, 711, 922, 1020, 1212, 1346, 1502, 2276
BseMI GCAATG 4 cut(s) 1179, 1362, 2176, 2203
BseMII CTCAG 8 cut(s) 205, 239, 401, 603, 641, 927, 1632, 1667
BseNI ACTGG 5 cut(s) 530, 904, 1163, 1325, 2015
BseRI GAGGAG 5 cut(s) 795, 1821, 1925, 1928, 2063
BseX3I CGGCCG 1 cut(s) 1804
BseXI GCAGC 5 cut(s) 470, 677, 961, 1983, 2318
BseYI CCCAGC 2 cut(s) 1593, 2243
BsgI GTGCAG 1 cut(s) 470
Bsh1236I CGCG 2 cut(s) 1193, 2117
Bsh1285I CGRYCG 2 cut(s) 1191, 1807
BshFI GGCC 5 cut(s) 28, 1324, 1534, 1611, 1806
BshNI GGYRCC 1 cut(s) 1432
BshTI ACCGGT 1 cut(s) 1760
BsiEI CGRYCG 2 cut(s) 1191, 1807
BsiHKAI GWGCWC 3 cut(s) 1230, 1521, 1654
BsiSI CCGG 3 cut(s) 909, 1566, 1761
BslFI GGGAC 8 cut(s) 242, 845, 976, 1041, 1503, 1611, 2175, 2216
BslI CCNNNNNNNGG 9 cut(s) 110, 368, 711, 922, 1020, 1212, 1346, 1502, 2276
BsmAI GTCTC 6 cut(s) 906, 1173, 1307, 1731, 1953, 2060
BsmFI GGGAC 8 cut(s) 242, 845, 976, 1041, 1503, 1611, 2175, 2216
BsnI GGCC 5 cut(s) 28, 1324, 1534, 1611, 1806
Bso31I GGTCTC 2 cut(s) 906, 1731
Bsp119I TTCGAA 1 cut(s) 1753
Bsp1286I GDGCHC 5 cut(s) 718, 1230, 1521, 1654, 1696
Bsp1407I TGTACA 2 cut(s) 1635, 2225
Bsp143I GATC 9 cut(s) 162, 275, 498, 526, 1388, 1941, 2097, 2200, 2266
Bsp19I CCATGG 4 cut(s) 226, 494, 739, 1002
BspACI CCGC 3 cut(s) 1584, 2025, 2117
BspANI GGCC 5 cut(s) 28, 1324, 1534, 1611, 1806
BspCNI CTCAG 8 cut(s) 204, 240, 400, 604, 640, 928, 1633, 1666
BspFNI CGCG 2 cut(s) 1193, 2117
BspHI TCATGA 1 cut(s) 97
BspLI GGNNCC 8 cut(s) 224, 258, 804, 833, 834, 1434, 1917, 2149
BspMAI CTGCAG 1 cut(s) 1973
BspMI ACCTGC 1 cut(s) 1444
BspPI GGATC 5 cut(s) 157, 506, 521, 1396, 2261
BspT104I TTCGAA 1 cut(s) 1753
BspT107I GGYRCC 1 cut(s) 1432
BspTNI GGTCTC 2 cut(s) 906, 1731
BsrDI GCAATG 4 cut(s) 1179, 1362, 2176, 2203
BsrFI RCCGGY 1 cut(s) 1760
BsrGI TGTACA 2 cut(s) 1635, 2225
BsrI ACTGG 5 cut(s) 530, 904, 1163, 1325, 2015
BssAI RCCGGY 1 cut(s) 1760
BssECI CCNNGG 9 cut(s) 226, 259, 494, 705, 739, 959, 1002, 1552, 2275
BssMI GATC 9 cut(s) 162, 275, 498, 526, 1388, 1941, 2097, 2200, 2266
BssT1I CCWWGG 6 cut(s) 226, 494, 705, 739, 1002, 1552
Bst2UI CCWGG 6 cut(s) 167, 261, 960, 1995, 2046, 2277
Bst4CI ACNGT 7 cut(s) 1015, 1084, 1189, 1400, 1951, 2081, 2230
Bst6I CTCTTC 2 cut(s) 1729, 2255
BstAUI TGTACA 2 cut(s) 1635, 2225
BstBI TTCGAA 1 cut(s) 1753
BstC8I GCNNGC 9 cut(s) 468, 509, 600, 1238, 1609, 1920, 2030, 2119, 2311
BstDSI CCRYGG 4 cut(s) 226, 494, 739, 1002
BstEII GGTNACC 1 cut(s) 644
BstENI CCTNNNNNAGG 1 cut(s) 108
BstF5I GGATG 7 cut(s) 207, 349, 523, 1062, 1202, 1821, 1863
BstFNI CGCG 2 cut(s) 1193, 2117
BstHHI GCGC 1 cut(s) 1195
BstKTI GATC 9 cut(s) 165, 278, 501, 529, 1391, 1944, 2100, 2203, 2269
BstMAI GTCTC 6 cut(s) 906, 1173, 1307, 1731, 1953, 2060
BstMBI GATC 9 cut(s) 162, 275, 498, 526, 1388, 1941, 2097, 2200, 2266
BstMCI CGRYCG 2 cut(s) 1191, 1807
BstMWI GCNNNNNNNGC 5 cut(s) 467, 983, 1190, 2180, 2303
BstNI CCWGG 6 cut(s) 167, 261, 960, 1995, 2046, 2277
BstNSI RCATGY 1 cut(s) 2003
BstPAI GACNNNNGTC 2 cut(s) 1085, 1287
BstPI GGTNACC 1 cut(s) 644
BstSCI CCNGG 7 cut(s) 165, 259, 907, 958, 1993, 2044, 2275
BstSFI CTRYAG 1 cut(s) 1969
BstUI CGCG 2 cut(s) 1193, 2117
BstV1I GCAGC 5 cut(s) 470, 677, 961, 1983, 2318
BstV2I GAAGAC 4 cut(s) 227, 678, 1081, 2294
BstXI CCANNNNNNTGG 1 cut(s) 173
BstZI CGGCCG 1 cut(s) 1804
Bsu36I CCTNAGG 1 cut(s) 191
BsuI GTATCC 1 cut(s) 1120
BsuRI GGCC 5 cut(s) 28, 1324, 1534, 1611, 1806
BtgI CCRYGG 4 cut(s) 226, 494, 739, 1002
BtsCI GGATG 7 cut(s) 207, 349, 523, 1062, 1202, 1821, 1863
BtsI GCAGTG 1 cut(s) 458
BtsIMutI CAGTG 4 cut(s) 458, 537, 1638, 2107
BveI ACCTGC 1 cut(s) 1444
Cac8I GCNNGC 9 cut(s) 468, 509, 600, 1238, 1609, 1920, 2030, 2119, 2311
CaiI CAGNNNCTG 1 cut(s) 2045
CciI TCATGA 1 cut(s) 97
CfoI GCGC 1 cut(s) 1195
Cfr10I RCCGGY 1 cut(s) 1760
Cfr13I GGNCC 6 cut(s) 200, 256, 371, 578, 832, 1532
CspAI ACCGGT 1 cut(s) 1760
CspCI CAANNNNNGTGG 2 cut(s) 98, 133
DpnI GATC 9 cut(s) 164, 277, 500, 528, 1390, 1943, 2099, 2202, 2268
DpnII GATC 9 cut(s) 162, 275, 498, 526, 1388, 1941, 2097, 2200, 2266
DraI TTTAAA 1 cut(s) 2155
EaeI YGGCCR 1 cut(s) 1804
EagI CGGCCG 1 cut(s) 1804
Eam1104I CTCTTC 2 cut(s) 1729, 2255
EarI CTCTTC 2 cut(s) 1729, 2255
Ecl136II GAGCTC 2 cut(s) 1228, 1652
EclXI CGGCCG 1 cut(s) 1804
Eco130I CCWWGG 6 cut(s) 226, 494, 705, 739, 1002, 1552
Eco24I GRGCYC 4 cut(s) 718, 1230, 1654, 1696
Eco31I GGTCTC 2 cut(s) 906, 1731
Eco47I GGWCC 5 cut(s) 200, 256, 371, 578, 832
Eco52I CGGCCG 1 cut(s) 1804
Eco53kI GAGCTC 2 cut(s) 1228, 1652
Eco57I CTGAAG 3 cut(s) 59, 1713, 2199
Eco81I CCTNAGG 1 cut(s) 191
Eco91I GGTNACC 1 cut(s) 644
EcoICRI GAGCTC 2 cut(s) 1228, 1652
EcoNI CCTNNNNNAGG 1 cut(s) 108
EcoO109I RGGNCCY 4 cut(s) 256, 578, 832, 1532
EcoO65I GGTNACC 1 cut(s) 644
EcoRI GAATTC 2 cut(s) 1335, 2111
EcoRII CCWGG 6 cut(s) 165, 259, 958, 1993, 2044, 2275
EcoT14I CCWWGG 6 cut(s) 226, 494, 705, 739, 1002, 1552
EcoT22I ATGCAT 1 cut(s) 1264
EcoT38I GRGCYC 4 cut(s) 718, 1230, 1654, 1696
ErhI CCWWGG 6 cut(s) 226, 494, 705, 739, 1002, 1552
FalI AAGNNNNNCTT 2 cut(s) 1726, 1758
FaqI GGGAC 8 cut(s) 242, 845, 976, 1041, 1503, 1611, 2175, 2216
FauI CCCGC 1 cut(s) 2110
FauNDI CATATG 1 cut(s) 445
Fnu4HI GCNGC 7 cut(s) 459, 666, 975, 1972, 2026, 2307, 2314
FokI GGATG 7 cut(s) 194, 356, 530, 1049, 1189, 1828, 1850
FriOI GRGCYC 4 cut(s) 718, 1230, 1654, 1696
Fsp4HI GCNGC 7 cut(s) 459, 666, 975, 1972, 2026, 2307, 2314
FspBI CTAG 6 cut(s) 1143, 1275, 1341, 1451, 1553, 2294
GlaI GCGC 1 cut(s) 1194
GluI GCNGC 7 cut(s) 459, 666, 975, 1972, 2026, 2307, 2314
GsaI CCCAGC 2 cut(s) 1597, 2247
HaeIII GGCC 5 cut(s) 28, 1324, 1534, 1611, 1806
HapII CCGG 3 cut(s) 909, 1566, 1761
HhaI GCGC 1 cut(s) 1195
Hin6I GCGC 1 cut(s) 1193
HinP1I GCGC 1 cut(s) 1193
HincII GTYRAC 1 cut(s) 1954
HindII GTYRAC 1 cut(s) 1954
HinfI GANTC 4 cut(s) 70, 264, 844, 1491
HpaII CCGG 3 cut(s) 909, 1566, 1761
HphI GGTGA 7 cut(s) 157, 259, 656, 839, 1631, 1798, 2276
Hpy166II GTNNAC 5 cut(s) 319, 1269, 1636, 1638, 1954
Hpy188I TCNGA 6 cut(s) 21, 39, 275, 280, 390, 1656
Hpy188III TCNNGA 9 cut(s) 98, 524, 576, 809, 1172, 1731, 2132, 2177, 2284
Hpy8I GTNNAC 5 cut(s) 319, 1269, 1636, 1638, 1954
Hpy99I CGWCG 1 cut(s) 2198
HpyCH4III ACNGT 7 cut(s) 1015, 1084, 1189, 1400, 1951, 2081, 2230
HpyF10VI GCNNNNNNNGC 5 cut(s) 467, 983, 1190, 2180, 2303
HspAI GCGC 1 cut(s) 1193
KflI GGGWCCC 1 cut(s) 832
Kzo9I GATC 9 cut(s) 162, 275, 498, 526, 1388, 1941, 2097, 2200, 2266
Lsp1109I GCAGC 5 cut(s) 470, 677, 961, 1983, 2318
LweI GCATC 4 cut(s) 508, 1071, 1711, 1809
MaeI CTAG 6 cut(s) 1143, 1275, 1341, 1451, 1553, 2294
MaeIII GTNAC 6 cut(s) 644, 788, 845, 1135, 1543, 1945
MalI GATC 9 cut(s) 164, 277, 500, 528, 1390, 1943, 2099, 2202, 2268
MboI GATC 9 cut(s) 162, 275, 498, 526, 1388, 1941, 2097, 2200, 2266
MhlI GDGCHC 5 cut(s) 718, 1230, 1521, 1654, 1696
MluCI AATT 9 cut(s) 616, 751, 1018, 1104, 1120, 1335, 1891, 2111, 2156
MlyI GAGTC 2 cut(s) 853, 1485
MmeI TCCRAC 3 cut(s) 178, 439, 2083
Mph1103I ATGCAT 1 cut(s) 1264
MroXI GAANNNNTTC 2 cut(s) 1418, 2218
MseI TTAA 7 cut(s) 537, 1017, 1881, 1980, 2004, 2154, 2320
MslI CAYNNNNRTG 2 cut(s) 1484, 1524
MspI CCGG 3 cut(s) 909, 1566, 1761
MspR9I CCNGG 7 cut(s) 167, 261, 909, 960, 1995, 2046, 2277
MvaI CCWGG 6 cut(s) 167, 261, 960, 1995, 2046, 2277
MvnI CGCG 2 cut(s) 1193, 2117
MwoI GCNNNNNNNGC 5 cut(s) 467, 983, 1190, 2180, 2303
NciI CCSGG 1 cut(s) 909
NcoI CCATGG 4 cut(s) 226, 494, 739, 1002
NdeI CATATG 1 cut(s) 445
NdeII GATC 9 cut(s) 162, 275, 498, 526, 1388, 1941, 2097, 2200, 2266
NlaIV GGNNCC 8 cut(s) 224, 258, 804, 833, 834, 1434, 1917, 2149
NmuCI GTSAC 2 cut(s) 644, 845
NsiI ATGCAT 1 cut(s) 1264
NspI RCATGY 1 cut(s) 2003
NspV TTCGAA 1 cut(s) 1753
PagI TCATGA 1 cut(s) 97
PaqCI CACCTGC 1 cut(s) 1444
PciI ACATGT 1 cut(s) 1999
PdmI GAANNNNTTC 2 cut(s) 1418, 2218
PfeI GAWTC 2 cut(s) 70, 264
PflMI CCANNNNNTGG 2 cut(s) 922, 1502
PfoI TCCNGGA 4 cut(s) 165, 907, 1993, 2044
PinAI ACCGGT 1 cut(s) 1760
PkrI GCNGC 7 cut(s) 460, 667, 976, 1973, 2027, 2308, 2315
PleI GAGTC 2 cut(s) 852, 1485
PpsI GAGTC 2 cut(s) 852, 1485
PpuMI RGGWCCY 3 cut(s) 256, 578, 832
PscI ACATGT 1 cut(s) 1999
PshAI GACNNNNGTC 2 cut(s) 1085, 1287
PshBI ATTAAT 1 cut(s) 537
Psp124BI GAGCTC 2 cut(s) 1230, 1654
Psp5II RGGWCCY 3 cut(s) 256, 578, 832
Psp6I CCWGG 6 cut(s) 165, 259, 958, 1993, 2044, 2275
PspEI GGTNACC 1 cut(s) 644
PspFI CCCAGC 2 cut(s) 1593, 2243
PspGI CCWGG 6 cut(s) 165, 259, 958, 1993, 2044, 2275
PspN4I GGNNCC 8 cut(s) 224, 258, 804, 833, 834, 1434, 1917, 2149
PspPI GGNCC 6 cut(s) 200, 256, 371, 578, 832, 1532
PspPPI RGGWCCY 3 cut(s) 256, 578, 832
PstI CTGCAG 1 cut(s) 1973
PstNI CAGNNNCTG 1 cut(s) 2045
RseI CAYNNNNRTG 2 cut(s) 1484, 1524
SacI GAGCTC 2 cut(s) 1230, 1654
SaqAI TTAA 7 cut(s) 537, 1017, 1881, 1980, 2004, 2154, 2320
SatI GCNGC 7 cut(s) 459, 666, 975, 1972, 2026, 2307, 2314
Sau3AI GATC 9 cut(s) 162, 275, 498, 526, 1388, 1941, 2097, 2200, 2266
Sau96I GGNCC 6 cut(s) 200, 256, 371, 578, 832, 1532
ScaI AGTACT 1 cut(s) 2009
SchI GAGTC 2 cut(s) 853, 1485
ScrFI CCNGG 7 cut(s) 167, 261, 909, 960, 1995, 2046, 2277
SduI GDGCHC 5 cut(s) 718, 1230, 1521, 1654, 1696
SfaNI GCATC 4 cut(s) 508, 1071, 1711, 1809
SfcI CTRYAG 1 cut(s) 1969
SfuI TTCGAA 1 cut(s) 1753
SinI GGWCC 5 cut(s) 200, 256, 371, 578, 832
SmiMI CAYNNNNRTG 2 cut(s) 1484, 1524
SmlI CTYRAG 1 cut(s) 851
SmoI CTYRAG 1 cut(s) 851
Sse9I AATT 9 cut(s) 616, 751, 1018, 1104, 1120, 1335, 1891, 2111, 2156
SsiI CCGC 3 cut(s) 1584, 2025, 2117
SspI AATATT 1 cut(s) 484
SspMI CTAG 6 cut(s) 1143, 1275, 1341, 1451, 1553, 2294
SstI GAGCTC 2 cut(s) 1230, 1654
StyD4I CCNGG 7 cut(s) 165, 259, 907, 958, 1993, 2044, 2275
StyI CCWWGG 6 cut(s) 226, 494, 705, 739, 1002, 1552
TaaI ACNGT 7 cut(s) 1015, 1084, 1189, 1400, 1951, 2081, 2230
TaqI TCGA 9 cut(s) 126, 810, 998, 1428, 1575, 1629, 1753, 1854, 2196
TasI AATT 9 cut(s) 616, 751, 1018, 1104, 1120, 1335, 1891, 2111, 2156
TatI WGTACW 4 cut(s) 402, 1635, 2007, 2225
TauI GCSGC 1 cut(s) 2028
TfiI GAWTC 2 cut(s) 70, 264
Tru1I TTAA 7 cut(s) 537, 1017, 1881, 1980, 2004, 2154, 2320
Tru9I TTAA 7 cut(s) 537, 1017, 1881, 1980, 2004, 2154, 2320
TscAI CASTG 4 cut(s) 458, 537, 1645, 2107
TseFI GTSAC 2 cut(s) 644, 845
TseI GCWGC 6 cut(s) 458, 665, 974, 1971, 2306, 2313
Tsp45I GTSAC 2 cut(s) 644, 845
TspGWI ACGGA 2 cut(s) 564, 1281
TspRI CASTG 4 cut(s) 458, 537, 1645, 2107
Van91I CCANNNNNTGG 2 cut(s) 922, 1502
VpaK11BI GGWCC 5 cut(s) 200, 256, 371, 578, 832
VspI ATTAAT 1 cut(s) 537
XagI CCTNNNNNAGG 1 cut(s) 108
XapI RAATTY 4 cut(s) 751, 1335, 1891, 2111
XceI RCATGY 1 cut(s) 2003
XmaJI CCTAGG 1 cut(s) 1552
XmnI GAANNNNTTC 2 cut(s) 1418, 2218
XspI CTAG 6 cut(s) 1143, 1275, 1341, 1451, 1553, 2294
ZrmI AGTACT 1 cut(s) 2009
Zsp2I ATGCAT 1 cut(s) 1264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.