Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3A

Orthogroup ID

OG0002763

Physical Location & Seq

Genomic Coordinates Forward (+)

19356057 .. 19360035

Gene Structure 3979 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3AG197300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2307 bp

ATGTGGCCTGATCTTCTCCGAAAGGCCAAACAGGGCGGCTTGAATGTGATTCAGACCTATGTGTTCTGGAACATTCATGAGCCTGTGCAAGGTCAGTGCAATTTCAAAGGGAACTATGATTTGGTGAAGTTCATAAAACTAGTTCAGAAGCATGGAATGTATGTGACGCTTAGAGTAGGGCCATTCATCCAAGCCGAATGGAACCACGGAGGACTCCCATATTGGCTCAGAGAAGTCCCGAATCTCCTATTCCGTACCGACAACGCACCGTACAAGTACTACATGAAGAAGTATGTGACCATGATTGTGAAAAGGATGAGGGAGGCGAAGCTATTTAGTCCACAGGGAGGTCCCATTATATTAGCACAAATTGAAAACGAGTACAATCATATCCAACTGGCGTATAAAGAGATGGGATACAGTTACGTCCGATGGGCAGCGAACATGGCGGTGAAGCAGGACGCCGGAGTACCATGGATCATGTGCAAGCAGATGGACGCTCCGGATCCGGTGATCAATACTTGCAACGGAAGGCATTGTGGTGATACTTTCCAAGGGCCTAATAAGCCTAACAAGCCTGCTCTATGGACCGAAAACTGGACTGCTCAATACCGAGTATTTGGTGACCCTCCTTCCCAGAGGCCAGTGGAAGACATTGCATATTCGGTTGCTCGTTTTGTTTCGAAAAATGGGGTTCTAACAAACTATTACATGTACCATGGAGGGACTAATTTTGGCAGAACAAGCGCCATTTTCACGACGACTCGCTACTACGACGAGGCTCCCGTTGACGAATATGGTCTGCCTAGAGACCCTAAATGGAGTCACCTTAGGGACTTACACAAGGCTTTGAAACTATGTAAGAAGCCTTTGCTTTGGGGAGTTTCTGGAGTCCAGAAGCTAGGCAAGGACACCGAGGCTCGTTTCTATGAGTTGAAGGACAACTCCACAATCTGTGCTGCCTTCTTGGCCAACAGTAACTCAAGAACTGAAGCAATTGTTAATTGGCGAGGTCAGGAATACTACCTGCCTCCTCATTCGATTAGCATTCTCCCCGATTGCAAGACTGTCGTCTTCAATACTCAAACAATTGTGTCGCAACACAACGCGAGGAACTTTGTTAGATCAAAGGTTGCCAATAATTTGAAATGGAAGAAGTCTCAGGAACCAATTCCAAGCATCGTTCAAGTTCCTATCAATAGCAAGACACCTCTAGAGCTTTACAACTTGTTAAAAGATGCTTCAGACTACGCATGGTACACCACCACCCTTCAGTTGAGCAGGCGTGACTTGCCCATGAAGGCAACTATTAAACCAGTTCTACGAATTGCAAATCTCGGTCATGCAATGCTTGTGTTTGTTAATGGCAAATATGTTGGTTCGGGACATGGGAGCCACGACGAGAAGAGCTTCATCTTCCAGAAAACGGTTAGCTTTACGGAGGGCGTCAACGAAATAGGACTATTTGCCATGACAGTGGGGCTCCCAGACAGCGGAGCCTACATGGAGCACAGGTACGCAGGGCCTCGAGAGATTATAGTTCTAGGGCTGAACACAGGAACCTTAGATCTCTCACAAAATGGGTGGGGACATCATATTGGTCTGAACGGAGAAAAAGTAAAACTGTTCACCGAGGAGGGATCCAAACAAGTTCAATGGAGCAATACAAAAGGAGCAGCTCAGGCACTAACATGGTACAAGACATATTTTGATGCTCCAGAAGGACGTGACCCGGTTGCAATCCGAATGACAGGCATGGGAAAAGGAATGATTTGGGTAAATGGCCAAAGTATTGGTCGACACTGGATGTCCTTCCTTTCTCCACTCGGGCAACCTACTCAATCAGAATACCATATCCCGAGATCCTTCATTAAACCTAAACAAAATTTTCTTGTTGTATTGGAAGAGGAGCCGATGAAGCCTGATGACATTGAGATCCTAACAGTCAACAGGGACACAATCTGTAGCTTCATTACTGAGTATCATCCACCAAACGTAAAGTCGTGGGAAAGAAAAAATAGCCATTTTCGACCAGTGTTGGAGGTTGTCAGAACTTCGACGGAGCTCAAGTGCCCGAACTACAAAAAGATTGTTTCTGTTGAGTTTGCAAGCTTTGGTGATCCAACTGGGGTATGTGGAAGCTATGTCTTGGGTAACTGCAACTCCCCTATATCCAAAGAGACTGTAGAACAACATTGCTTGGGAAAATCTAGCTGCGCAGTCCCGATTGACGACAAGCTTTTCTTTAAGAAAAATGATGCGTGCCCAGATATCAAGAAGACACTTGCTGCTCAAGTAAATTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BetaGal_dom4_5 Gal_Lectin Glyco_hydro_35

Protein Analysis

768

Amino Acids

87.17

Weight (kDa)

9.16

Isoelectric Point (pI)

36.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	1 - 283	6.5e-103	Glycosyl hydrolases family 35
GHD	PF17834	308 - 363	2.3e-20	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	563 - 629	6.7e-25	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	689 - 766	7.8e-15	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002763)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16730 AT4G35010
fragaria_vesca	FvH4_2g01270 FvH4_4g13900
malus_domestica	MD04G1010100.v1.1 MD05G1121000.v1.1 MD10G1095200.v1.1 MD13G1284600.v1.1
prunus_persica	Prupe.1G009200_v2.0.a1 Prupe.1G009300_v2.0.a1 Prupe.8G164800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0475241 RchiOBHm_Chr4g0415021 RchiOBHm_Chr6g0251931
rosa_laevigata	RLG00000008102 RLG00000015418 RLG00000023857
rosa_multiflora	Rmu_sc0003611.1_g000013 Rmu_sc0008157.1_g000007 Rmu_ssc0000303.1_g000015
rosa_roxburghii	Rroxscaffold_5G00358760 Rroxscaffold_6G00406440
rosa_rugosa	Rorug03G0147300 Rorug04G0131700 Rorug05G0505100 Rorug05G0505200 Rorug05G0505200
rosa_samantha	Rh3AG197300 Rh4AG191000 Rh4BG189100 Rh4CG202000 Rh4DG189500 Rh6AG016400 Rh6BG014400 Rh6CG011300 Rh6DG012600
rosa_wichuraiana	Rw3G017940 Rw4G016250 Rw6G001570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	2218
Acc36I	ACCTGC	1 cut(s)	1035
AccI	GTMKAC	1 cut(s)	1798
AccII	CGCG	1 cut(s)	1109
AccIII	TCCGGA	1 cut(s)	502
AciI	CCGC	3 cut(s)	36, 449, 1494
AclWI	GGATC	8 cut(s)	485, 500, 513, 1635, 1648, 1857, 1930, 2114
AcoI	YGGCCR	2 cut(s)	969, 1783
AcsI	RAATTY	2 cut(s)	1885, 2299
AcuI	CTGAAG	3 cut(s)	1011, 1227, 1256
AcyI	GRCGYC	2 cut(s)	462, 1446
AfaI	GTAC	9 cut(s)	256, 272, 278, 383, 471, 716, 1259, 1517, 1697
AfiI	CCNNNNNNNGG	5 cut(s)	89, 347, 597, 1426, 1493
AflIII	ACRYGT	1 cut(s)	711
AgsI	TTSAA	9 cut(s)	43, 106, 374, 853, 937, 1078, 1147, 1187, 1655
AhlI	ACTAGT	1 cut(s)	139
AjiI	CACGTC	1 cut(s)	1727
AjuI	GAANNNNNNNTTGG	2 cut(s)	104, 136
Alw21I	GWGCWC	2 cut(s)	1512, 2067
Alw26I	GTCTC	3 cut(s)	804, 1164, 2174
AlwI	GGATC	8 cut(s)	485, 500, 513, 1635, 1648, 1857, 1930, 2114
Ama87I	CYCGRG	3 cut(s)	1527, 1826, 1858
Aor13HI	TCCGGA	1 cut(s)	502
AoxI	GGCC	8 cut(s)	5, 24, 179, 557, 641, 969, 1523, 1783
ApeKI	GCWGC	5 cut(s)	437, 959, 1676, 2214, 2288
ApoI	RAATTY	2 cut(s)	1885, 2299
ArsI	GACNNNNNNTTYG	4 cut(s)	1780, 1812, 1985, 2017
Asp700I	GAANNNNTTC	2 cut(s)	1170, 1409
AspLEI	GCGC	2 cut(s)	749, 2219
AspS9I	GGNCC	5 cut(s)	179, 350, 557, 588, 1523
AsuC2I	CCSGG	1 cut(s)	1733
AsuHPI	GGTGA	8 cut(s)	136, 463, 523, 554, 635, 818, 1621, 2129
AsuII	TTCGAA	1 cut(s)	683
AvaI	CYCGRG	3 cut(s)	1527, 1826, 1858
AvaII	GGWCC	2 cut(s)	350, 588
AxyI	CCTNAGG	1 cut(s)	830
BaeGI	GKGCMC	2 cut(s)	2075, 2267
BalI	TGGCCA	2 cut(s)	971, 1785
BamHI	GGATCC	2 cut(s)	505, 1640
BanII	GRGCYC	2 cut(s)	1485, 2067
BbsI	GAAGAC	3 cut(s)	657, 1066, 2285
Bbv12I	GWGCWC	2 cut(s)	1512, 2067
BbvI	GCAGC	5 cut(s)	449, 946, 1688, 2201, 2275
BccI	CCATC	3 cut(s)	406, 426, 487
BcgI	CGANNNNNNTGC	2 cut(s)	1051, 1085
BciVI	GTATCC	1 cut(s)	410
BclI	TGATCA	1 cut(s)	513
BcnI	CCSGG	1 cut(s)	1733
BcoDI	GTCTC	3 cut(s)	804, 1164, 2174
BcuI	ACTAGT	1 cut(s)	139
BfaI	CTAG	6 cut(s)	140, 807, 902, 1214, 1544, 2211
BfmI	CTRYAG	2 cut(s)	1963, 2184
BfoI	RGCGCY	1 cut(s)	750
BfuAI	ACCTGC	1 cut(s)	1035
BfuI	GTATCC	1 cut(s)	410
BglI	GCCNNNNNGGC	1 cut(s)	968
BglII	AGATCT	1 cut(s)	1567
BisI	GCNGC	6 cut(s)	37, 438, 960, 1677, 2215, 2289
BlsI	GCNGC	6 cut(s)	38, 439, 961, 1678, 2216, 2290
BmcAI	AGTACT	1 cut(s)	278
Bme1390I	CCNGG	1 cut(s)	1733
Bme18I	GGWCC	2 cut(s)	350, 588
BmeT110I	CYCGRG	3 cut(s)	1527, 1826, 1858
BmgBI	CACGTC	1 cut(s)	1727
BmgT120I	GGNCC	5 cut(s)	179, 350, 557, 588, 1523
BmrFI	CCNGG	1 cut(s)	1733
BmrI	ACTGGG	1 cut(s)	2136
BmsI	GCATC	4 cut(s)	1188, 1228, 1702, 2248
BmuI	ACTGGG	1 cut(s)	2136
BoxI	GACNNNNGTC	1 cut(s)	1070
BpiI	GAAGAC	3 cut(s)	657, 1066, 2285
BpmI	CTGGAG	2 cut(s)	909, 1701
Bpu10I	CCTNAGC	1 cut(s)	1680
Bpu14I	TTCGAA	1 cut(s)	683
BpuEI	CTTGAG	3 cut(s)	967, 2051, 2277
BpuMI	CCSGG	1 cut(s)	1733
BsaHI	GRCGYC	2 cut(s)	462, 1446
BsaI	GGTCTC	1 cut(s)	804
BsaJI	CCNNGG	6 cut(s)	205, 473, 553, 718, 915, 1632
BsaWI	WCCGGW	2 cut(s)	502, 508
BsaXI	ACNNNNNCTCC	8 cut(s)	1499, 1529, 1602, 1632, 2054, 2084, 2147, 2177
Bsc4I	CCNNNNNNNGG	5 cut(s)	89, 347, 597, 1426, 1493
Bse1I	ACTGG	7 cut(s)	402, 602, 644, 1316, 1808, 2033, 2131
Bse21I	CCTNAGG	1 cut(s)	830
Bse3DI	GCAATG	3 cut(s)	654, 1353, 2194
BseAI	TCCGGA	1 cut(s)	502
BseDI	CCNNGG	6 cut(s)	205, 473, 553, 718, 915, 1632
BseGI	GGATG	4 cut(s)	186, 321, 1812, 1984
BseLI	CCNNNNNNNGG	5 cut(s)	89, 347, 597, 1426, 1493
BseMI	GCAATG	3 cut(s)	654, 1353, 2194
BseMII	CTCAG	4 cut(s)	241, 1175, 1694, 1968
BseNI	ACTGG	7 cut(s)	402, 602, 644, 1316, 1808, 2033, 2131
BseRI	GAGGAG	3 cut(s)	1023, 1649, 1922
BseSI	GKGCMC	2 cut(s)	2075, 2267
BseXI	GCAGC	5 cut(s)	449, 946, 1688, 2201, 2275
Bsh1236I	CGCG	1 cut(s)	1109
BshFI	GGCC	8 cut(s)	7, 26, 181, 559, 643, 971, 1525, 1785
BsiHKAI	GWGCWC	2 cut(s)	1512, 2067
BsiHKCI	CYCGRG	3 cut(s)	1527, 1826, 1858
BsiSI	CCGG	4 cut(s)	465, 503, 509, 1733
BslFI	GGGAC	8 cut(s)	221, 336, 739, 848, 1398, 1602, 1967, 2207
BslI	CCNNNNNNNGG	5 cut(s)	89, 347, 597, 1426, 1493
BsmAI	GTCTC	3 cut(s)	804, 1164, 2174
BsmFI	GGGAC	8 cut(s)	221, 336, 739, 848, 1398, 1602, 1967, 2207
BsmI	GAATGC	1 cut(s)	1047
BsnI	GGCC	8 cut(s)	7, 26, 181, 559, 643, 971, 1525, 1785
Bso31I	GGTCTC	1 cut(s)	804
BsoBI	CYCGRG	3 cut(s)	1527, 1826, 1858
Bsp119I	TTCGAA	1 cut(s)	683
Bsp1286I	GDGCHC	5 cut(s)	1485, 1512, 2067, 2075, 2267
Bsp13I	TCCGGA	1 cut(s)	502
Bsp19I	CCATGG	2 cut(s)	473, 718
BspACI	CCGC	3 cut(s)	36, 449, 1494
BspANI	GGCC	8 cut(s)	7, 26, 181, 559, 643, 971, 1525, 1785
BspCNI	CTCAG	4 cut(s)	240, 1174, 1693, 1969
BspEI	TCCGGA	1 cut(s)	502
BspFNI	CGCG	1 cut(s)	1109
BspHI	TCATGA	1 cut(s)	76
BspMI	ACCTGC	1 cut(s)	1035
BspPI	GGATC	8 cut(s)	485, 500, 513, 1635, 1648, 1857, 1930, 2114
BspQI	GCTCTTC	1 cut(s)	1400
BspT104I	TTCGAA	1 cut(s)	683
BspTNI	GGTCTC	1 cut(s)	804
BsrDI	GCAATG	3 cut(s)	654, 1353, 2194
BsrI	ACTGG	7 cut(s)	402, 602, 644, 1316, 1808, 2033, 2131
BssECI	CCNNGG	6 cut(s)	205, 473, 553, 718, 915, 1632
BssNI	GRCGYC	2 cut(s)	462, 1446
BssT1I	CCWWGG	3 cut(s)	473, 553, 718
Bst4CI	ACNGT	9 cut(s)	270, 422, 977, 1069, 1429, 1477, 1626, 1945, 2185
Bst6I	CTCTTC	2 cut(s)	1400, 1899
BstACI	GRCGYC	2 cut(s)	462, 1446
BstBI	TTCGAA	1 cut(s)	683
BstC8I	GCNNGC	5 cut(s)	488, 579, 1283, 2110, 2263
BstDEI	CTNAG	7 cut(s)	170, 227, 830, 1161, 1564, 1680, 1977
BstDSI	CCRYGG	3 cut(s)	205, 473, 718
BstEII	GGTNACC	1 cut(s)	623
BstENI	CCTNNNNNAGG	1 cut(s)	87
BstF5I	GGATG	4 cut(s)	186, 321, 1812, 1984
BstFNI	CGCG	1 cut(s)	1109
BstH2I	RGCGCY	1 cut(s)	750
BstHHI	GCGC	2 cut(s)	749, 2219
BstMAI	GTCTC	3 cut(s)	804, 1164, 2174
BstMWI	GCNNNNNNNGC	8 cut(s)	446, 565, 574, 744, 968, 1291, 1682, 1918
BstNSI	RCATGY	1 cut(s)	715
BstPAI	GACNNNNGTC	1 cut(s)	1070
BstPI	GGTNACC	1 cut(s)	623
BstSCI	CCNGG	1 cut(s)	1731
BstSFI	CTRYAG	2 cut(s)	1963, 2184
BstSLI	GKGCMC	2 cut(s)	2075, 2267
BstUI	CGCG	1 cut(s)	1109
BstV1I	GCAGC	5 cut(s)	449, 946, 1688, 2201, 2275
BstV2I	GAAGAC	3 cut(s)	657, 1066, 2285
BstX2I	RGATCY	5 cut(s)	505, 1567, 1640, 1862, 1935
BstXI	CCANNNNNNTGG	2 cut(s)	1477, 1793
BstYI	RGATCY	5 cut(s)	505, 1567, 1640, 1862, 1935
Bsu36I	CCTNAGG	1 cut(s)	830
BsuI	GTATCC	1 cut(s)	410
BsuRI	GGCC	8 cut(s)	7, 26, 181, 559, 643, 971, 1525, 1785
BtgI	CCRYGG	3 cut(s)	205, 473, 718
BtrI	CACGTC	1 cut(s)	1727
BtsCI	GGATG	4 cut(s)	186, 321, 1812, 1984
BtsIMutI	CAGTG	5 cut(s)	101, 651, 1482, 1801, 2040
BveI	ACCTGC	1 cut(s)	1035
Cac8I	GCNNGC	5 cut(s)	488, 579, 1283, 2110, 2263
CciI	TCATGA	1 cut(s)	76
CfoI	GCGC	2 cut(s)	749, 2219
Cfr13I	GGNCC	5 cut(s)	179, 350, 557, 588, 1523
CseI	GACGC	4 cut(s)	175, 470, 506, 1435
Csp6I	GTAC	9 cut(s)	255, 271, 277, 382, 470, 715, 1258, 1516, 1696
CspCI	CAANNNNNGTGG	2 cut(s)	1565, 1600
CviQI	GTAC	9 cut(s)	255, 271, 277, 382, 470, 715, 1258, 1516, 1696
DdeI	CTNAG	7 cut(s)	170, 227, 830, 1161, 1564, 1680, 1977
EaeI	YGGCCR	2 cut(s)	969, 1783
Eam1104I	CTCTTC	2 cut(s)	1400, 1899
EarI	CTCTTC	2 cut(s)	1400, 1899
Ecl136II	GAGCTC	1 cut(s)	2065
Eco130I	CCWWGG	3 cut(s)	473, 553, 718
Eco24I	GRGCYC	2 cut(s)	1485, 2067
Eco31I	GGTCTC	1 cut(s)	804
Eco32I	GATATC	1 cut(s)	2272
Eco47I	GGWCC	2 cut(s)	350, 588
Eco53kI	GAGCTC	1 cut(s)	2065
Eco57I	CTGAAG	3 cut(s)	1011, 1227, 1256
Eco81I	CCTNAGG	1 cut(s)	830
Eco88I	CYCGRG	3 cut(s)	1527, 1826, 1858
Eco91I	GGTNACC	1 cut(s)	623
EcoICRI	GAGCTC	1 cut(s)	2065
EcoNI	CCTNNNNNAGG	1 cut(s)	87
EcoO109I	RGGNCCY	3 cut(s)	350, 557, 1523
EcoO65I	GGTNACC	1 cut(s)	623
EcoRV	GATATC	1 cut(s)	2272
EcoT14I	CCWWGG	3 cut(s)	473, 553, 718
EcoT38I	GRGCYC	2 cut(s)	1485, 2067
ErhI	CCWWGG	3 cut(s)	473, 553, 718
FalI	AAGNNNNNCTT	2 cut(s)	2228, 2260
FaqI	GGGAC	8 cut(s)	221, 336, 739, 848, 1398, 1602, 1967, 2207
FbaI	TGATCA	1 cut(s)	513
FblI	GTMKAC	1 cut(s)	1798
Fnu4HI	GCNGC	6 cut(s)	37, 438, 960, 1677, 2215, 2289
FokI	GGATG	4 cut(s)	173, 328, 1819, 1971
FriOI	GRGCYC	2 cut(s)	1485, 2067
Fsp4HI	GCNGC	6 cut(s)	37, 438, 960, 1677, 2215, 2289
FspBI	CTAG	6 cut(s)	140, 807, 902, 1214, 1544, 2211
FspI	TGCGCA	1 cut(s)	2218
GlaI	GCGC	2 cut(s)	748, 2218
GluI	GCNGC	6 cut(s)	37, 438, 960, 1677, 2215, 2289
GsuI	CTGGAG	2 cut(s)	909, 1701
HaeII	RGCGCY	1 cut(s)	750
HaeIII	GGCC	8 cut(s)	7, 26, 181, 559, 643, 971, 1525, 1785
HapII	CCGG	4 cut(s)	465, 503, 509, 1733
HgaI	GACGC	4 cut(s)	175, 470, 506, 1435
HhaI	GCGC	2 cut(s)	749, 2219
Hin1I	GRCGYC	2 cut(s)	462, 1446
Hin6I	GCGC	2 cut(s)	747, 2217
HinP1I	GCGC	2 cut(s)	747, 2217
HincII	GTYRAC	4 cut(s)	790, 1450, 1799, 1948
HindII	GTYRAC	4 cut(s)	790, 1450, 1799, 1948
HindIII	AAGCTT	2 cut(s)	2110, 2237
HinfI	GANTC	6 cut(s)	49, 213, 241, 763, 823, 891
HpaII	CCGG	4 cut(s)	465, 503, 509, 1733
HphI	GGTGA	8 cut(s)	136, 463, 523, 554, 635, 818, 1621, 2129
Hpy166II	GTNNAC	7 cut(s)	341, 790, 1260, 1450, 1629, 1799, 1948
Hpy8I	GTNNAC	7 cut(s)	341, 790, 1260, 1450, 1629, 1799, 1948
Hpy99I	CGWCG	4 cut(s)	763, 779, 1403, 2062
HpyAV	CCTTC	9 cut(s)	525, 642, 931, 973, 1280, 1294, 1715, 1822, 1876
HpyCH4III	ACNGT	9 cut(s)	270, 422, 977, 1069, 1429, 1477, 1626, 1945, 2185
HpyCH4IV	ACGT	3 cut(s)	426, 1726, 1995
HpyF10VI	GCNNNNNNNGC	8 cut(s)	446, 565, 574, 744, 968, 1291, 1682, 1918
HpyF3I	CTNAG	7 cut(s)	170, 227, 830, 1161, 1564, 1680, 1977
HpySE526I	ACGT	3 cut(s)	426, 1726, 1995
Hsp92I	GRCGYC	2 cut(s)	462, 1446
HspAI	GCGC	2 cut(s)	747, 2217
Kpn2I	TCCGGA	1 cut(s)	502
Ksp22I	TGATCA	1 cut(s)	513
LguI	GCTCTTC	1 cut(s)	1400
Lsp1109I	GCAGC	5 cut(s)	449, 946, 1688, 2201, 2275
LweI	GCATC	4 cut(s)	1188, 1228, 1702, 2248
MaeI	CTAG	6 cut(s)	140, 807, 902, 1214, 1544, 2211
MaeII	ACGT	3 cut(s)	426, 1726, 1995
MaeIII	GTNAC	9 cut(s)	163, 295, 422, 623, 824, 977, 1286, 1727, 2153
MboII	GAAGA	9 cut(s)	5, 298, 662, 1066, 1165, 1408, 1417, 1916, 2290
MfeI	CAATTG	2 cut(s)	996, 1089
MflI	RGATCY	5 cut(s)	505, 1567, 1640, 1862, 1935
MhlI	GDGCHC	5 cut(s)	1485, 1512, 2067, 2075, 2267
MlsI	TGGCCA	2 cut(s)	971, 1785
MluNI	TGGCCA	2 cut(s)	971, 1785
MlyI	GAGTC	4 cut(s)	207, 757, 832, 900
MmeI	TCCRAC	3 cut(s)	418, 2019, 2147
Mox20I	TGGCCA	2 cut(s)	971, 1785
MroI	TCCGGA	1 cut(s)	502
MroXI	GAANNNNTTC	2 cut(s)	1170, 1409
MscI	TGGCCA	2 cut(s)	971, 1785
MseI	TTAA	6 cut(s)	1002, 1232, 1311, 1362, 1872, 2247
MslI	CAYNNNNRTG	5 cut(s)	305, 449, 540, 1475, 1690
Msp20I	TGGCCA	2 cut(s)	971, 1785
MspA1I	CMGCKG	1 cut(s)	1494
MspI	CCGG	4 cut(s)	465, 503, 509, 1733
MspR9I	CCNGG	1 cut(s)	1733
MunI	CAATTG	2 cut(s)	996, 1089
Mva1269I	GAATGC	1 cut(s)	1047
MvnI	CGCG	1 cut(s)	1109
MwoI	GCNNNNNNNGC	8 cut(s)	446, 565, 574, 744, 968, 1291, 1682, 1918
NciI	CCSGG	1 cut(s)	1733
NcoI	CCATGG	2 cut(s)	473, 718
NmuCI	GTSAC	6 cut(s)	163, 295, 623, 824, 1286, 1727
NsbI	TGCGCA	1 cut(s)	2218
NspI	RCATGY	1 cut(s)	715
NspV	TTCGAA	1 cut(s)	683
PaeR7I	CTCGAG	1 cut(s)	1527
PagI	TCATGA	1 cut(s)	76
PciI	ACATGT	1 cut(s)	711
PciSI	GCTCTTC	1 cut(s)	1400
PcsI	WCGNNNNNNNCGW	1 cut(s)	783
PctI	GAATGC	1 cut(s)	1047
PdmI	GAANNNNTTC	2 cut(s)	1170, 1409
PfeI	GAWTC	2 cut(s)	49, 241
PkrI	GCNGC	6 cut(s)	38, 439, 961, 1678, 2216, 2290
PleI	GAGTC	4 cut(s)	207, 757, 831, 899
PpsI	GAGTC	4 cut(s)	207, 757, 831, 899
PpuMI	RGGWCCY	1 cut(s)	350
PscI	ACATGT	1 cut(s)	711
PshAI	GACNNNNGTC	1 cut(s)	1070
Psp124BI	GAGCTC	1 cut(s)	2067
Psp5II	RGGWCCY	1 cut(s)	350
PspEI	GGTNACC	1 cut(s)	623
PspPI	GGNCC	5 cut(s)	179, 350, 557, 588, 1523
PspPPI	RGGWCCY	1 cut(s)	350
PsuI	RGATCY	5 cut(s)	505, 1567, 1640, 1862, 1935
RsaI	GTAC	9 cut(s)	256, 272, 278, 383, 471, 716, 1259, 1517, 1697
RsaNI	GTAC	9 cut(s)	255, 271, 277, 382, 470, 715, 1258, 1516, 1696
RseI	CAYNNNNRTG	5 cut(s)	305, 449, 540, 1475, 1690
SacI	GAGCTC	1 cut(s)	2067
SalI	GTCGAC	1 cut(s)	1797
SapI	GCTCTTC	1 cut(s)	1400
SaqAI	TTAA	6 cut(s)	1002, 1232, 1311, 1362, 1872, 2247
SatI	GCNGC	6 cut(s)	37, 438, 960, 1677, 2215, 2289
Sau96I	GGNCC	5 cut(s)	179, 350, 557, 588, 1523
ScaI	AGTACT	1 cut(s)	278
SchI	GAGTC	4 cut(s)	207, 757, 832, 900
ScrFI	CCNGG	1 cut(s)	1733
SduI	GDGCHC	5 cut(s)	1485, 1512, 2067, 2075, 2267
SfaNI	GCATC	4 cut(s)	1188, 1228, 1702, 2248
SfcI	CTRYAG	2 cut(s)	1963, 2184
Sfr274I	CTCGAG	1 cut(s)	1527
SfuI	TTCGAA	1 cut(s)	683
SinI	GGWCC	2 cut(s)	350, 588
SlaI	CTCGAG	1 cut(s)	1527
SmiMI	CAYNNNNRTG	5 cut(s)	305, 449, 540, 1475, 1690
SmlI	CTYRAG	4 cut(s)	982, 1527, 2066, 2292
SmoI	CTYRAG	4 cut(s)	982, 1527, 2066, 2292
SpeI	ACTAGT	1 cut(s)	139
SsiI	CCGC	3 cut(s)	36, 449, 1494
SspMI	CTAG	6 cut(s)	140, 807, 902, 1214, 1544, 2211
SstI	GAGCTC	1 cut(s)	2067
StyD4I	CCNGG	1 cut(s)	1731
StyI	CCWWGG	3 cut(s)	473, 553, 718
TaaI	ACNGT	9 cut(s)	270, 422, 977, 1069, 1429, 1477, 1626, 1945, 2185
TaiI	ACGT	3 cut(s)	429, 1729, 1998
TaqI	TCGA	6 cut(s)	683, 1040, 1528, 1798, 2029, 2057
TaqII	GACCGA	2 cut(s)	605, 1328
TatI	WGTACW	2 cut(s)	276, 381
TauI	GCSGC	1 cut(s)	39
TfiI	GAWTC	2 cut(s)	49, 241
Tru1I	TTAA	6 cut(s)	1002, 1232, 1311, 1362, 1872, 2247
Tru9I	TTAA	6 cut(s)	1002, 1232, 1311, 1362, 1872, 2247
TscAI	CASTG	5 cut(s)	101, 651, 1482, 1808, 2040
TseFI	GTSAC	6 cut(s)	163, 295, 623, 824, 1286, 1727
TseI	GCWGC	5 cut(s)	437, 959, 1676, 2214, 2288
Tsp45I	GTSAC	6 cut(s)	163, 295, 623, 824, 1286, 1727
TspDTI	ATGAA	9 cut(s)	65, 121, 175, 299, 1313, 1402, 1858, 1931, 1960
TspGWI	ACGGA	6 cut(s)	222, 242, 543, 1454, 1623, 2075
TspRI	CASTG	5 cut(s)	101, 651, 1482, 1808, 2040
VpaK11BI	GGWCC	2 cut(s)	350, 588
XagI	CCTNNNNNAGG	1 cut(s)	87
XapI	RAATTY	2 cut(s)	1885, 2299
XbaI	TCTAGA	1 cut(s)	1213
XceI	RCATGY	1 cut(s)	715
XhoI	CTCGAG	1 cut(s)	1527
XmiI	GTMKAC	1 cut(s)	1798
XmnI	GAANNNNTTC	2 cut(s)	1170, 1409
XspI	CTAG	6 cut(s)	140, 807, 902, 1214, 1544, 2211
ZrmI	AGTACT	1 cut(s)	278

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.