E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 405 |
| Acc65I | GGTACC | 1 cut(s) | 348 |
| AccB1I | GGYRCC | 2 cut(s) | 348, 658 |
| AciI | CCGC | 3 cut(s) | 277, 323, 563 |
| AclWI | GGATC | 3 cut(s) | 417, 763, 776 |
| AcoI | YGGCCR | 1 cut(s) | 220 |
| AcsI | RAATTY | 3 cut(s) | 131, 184, 355 |
| AdeI | CACNNNGTG | 1 cut(s) | 231 |
| AfaI | GTAC | 4 cut(s) | 181, 350, 668, 851 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 443 |
| AgsI | TTSAA | 5 cut(s) | 25, 167, 237, 538, 604 |
| AjnI | CCWGG | 1 cut(s) | 7 |
| Alw21I | GWGCWC | 4 cut(s) | 162, 533, 742, 879 |
| Alw26I | GTCTC | 2 cut(s) | 687, 780 |
| Alw44I | GTGCAC | 1 cut(s) | 529 |
| AlwI | GGATC | 3 cut(s) | 417, 763, 776 |
| Ama87I | CYCGRG | 1 cut(s) | 650 |
| AoxI | GGCC | 1 cut(s) | 220 |
| ApaLI | GTGCAC | 1 cut(s) | 529 |
| ApeKI | GCWGC | 2 cut(s) | 240, 406 |
| ApoI | RAATTY | 3 cut(s) | 131, 184, 355 |
| Asp718I | GGTACC | 1 cut(s) | 348 |
| AsuHPI | GGTGA | 1 cut(s) | 479 |
| AvaI | CYCGRG | 1 cut(s) | 650 |
| BaeGI | GKGCMC | 2 cut(s) | 215, 533 |
| BamHI | GGATCC | 1 cut(s) | 768 |
| BanI | GGYRCC | 2 cut(s) | 348, 658 |
| BanII | GRGCYC | 3 cut(s) | 162, 742, 879 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 704, 736 |
| BauI | CACGAG | 1 cut(s) | 401 |
| BbsI | GAAGAC | 2 cut(s) | 592, 622 |
| Bbv12I | GWGCWC | 4 cut(s) | 162, 533, 742, 879 |
| BbvI | GCAGC | 2 cut(s) | 227, 418 |
| BccI | CCATC | 5 cut(s) | 67, 498, 518, 549, 649 |
| BceAI | ACGGC | 1 cut(s) | 235 |
| BciT130I | CCWGG | 1 cut(s) | 9 |
| BciVI | GTATCC | 1 cut(s) | 204 |
| BclI | TGATCA | 1 cut(s) | 502 |
| BcoDI | GTCTC | 2 cut(s) | 687, 780 |
| BfaI | CTAG | 2 cut(s) | 35, 834 |
| BfmI | CTRYAG | 2 cut(s) | 385, 427 |
| BfuAI | ACCTGC | 1 cut(s) | 405 |
| BfuI | GTATCC | 1 cut(s) | 204 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 219 |
| BisI | GCNGC | 3 cut(s) | 241, 277, 407 |
| BlsI | GCNGC | 3 cut(s) | 242, 278, 408 |
| Bme1390I | CCNGG | 1 cut(s) | 9 |
| BmeT110I | CYCGRG | 1 cut(s) | 650 |
| BmiI | GGNNCC | 5 cut(s) | 6, 121, 350, 660, 770 |
| BmrFI | CCNGG | 1 cut(s) | 9 |
| BmsI | GCATC | 3 cut(s) | 290, 579, 789 |
| BpiI | GAAGAC | 2 cut(s) | 592, 622 |
| BpuEI | CTTGAG | 3 cut(s) | 332, 380, 863 |
| BsaWI | WCCGGW | 1 cut(s) | 172 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 443 |
| Bse118I | RCCGGY | 1 cut(s) | 345 |
| Bse1I | ACTGG | 2 cut(s) | 662, 898 |
| Bse3DI | GCAATG | 2 cut(s) | 151, 409 |
| BseBI | CCWGG | 1 cut(s) | 9 |
| BseGI | GGATG | 2 cut(s) | 594, 660 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 443 |
| BseMI | GCAATG | 2 cut(s) | 151, 409 |
| BseMII | CTCAG | 2 cut(s) | 239, 441 |
| BseNI | ACTGG | 2 cut(s) | 662, 898 |
| BseSI | GKGCMC | 2 cut(s) | 215, 533 |
| BseXI | GCAGC | 2 cut(s) | 227, 418 |
| BsgI | GTGCAG | 1 cut(s) | 275 |
| BshFI | GGCC | 1 cut(s) | 222 |
| BshNI | GGYRCC | 2 cut(s) | 348, 658 |
| BsiHKAI | GWGCWC | 4 cut(s) | 162, 533, 742, 879 |
| BsiHKCI | CYCGRG | 1 cut(s) | 650 |
| BsiSI | CCGG | 2 cut(s) | 173, 346 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 443 |
| BsmAI | GTCTC | 2 cut(s) | 687, 780 |
| BsmBI | CGTCTC | 1 cut(s) | 687 |
| BsmI | GAATGC | 1 cut(s) | 583 |
| BsnI | GGCC | 1 cut(s) | 222 |
| BsoBI | CYCGRG | 1 cut(s) | 650 |
| Bsp1286I | GDGCHC | 5 cut(s) | 162, 215, 533, 742, 879 |
| Bsp1407I | TGTACA | 2 cut(s) | 179, 666 |
| Bsp143I | GATC | 3 cut(s) | 422, 502, 768 |
| BspACI | CCGC | 3 cut(s) | 277, 323, 563 |
| BspANI | GGCC | 1 cut(s) | 222 |
| BspCNI | CTCAG | 2 cut(s) | 240, 442 |
| BspLI | GGNNCC | 5 cut(s) | 6, 121, 350, 660, 770 |
| BspMI | ACCTGC | 1 cut(s) | 405 |
| BspPI | GGATC | 3 cut(s) | 417, 763, 776 |
| BspQI | GCTCTTC | 1 cut(s) | 867 |
| BspT107I | GGYRCC | 2 cut(s) | 348, 658 |
| BsrDI | GCAATG | 2 cut(s) | 151, 409 |
| BsrFI | RCCGGY | 1 cut(s) | 345 |
| BsrGI | TGTACA | 2 cut(s) | 179, 666 |
| BsrI | ACTGG | 2 cut(s) | 662, 898 |
| BssAI | RCCGGY | 1 cut(s) | 345 |
| BssMI | GATC | 3 cut(s) | 422, 502, 768 |
| BssSI | CACGAG | 1 cut(s) | 401 |
| Bst2BI | CACGAG | 1 cut(s) | 401 |
| Bst2UI | CCWGG | 1 cut(s) | 9 |
| Bst4CI | ACNGT | 5 cut(s) | 598, 608, 621, 746, 791 |
| Bst6I | CTCTTC | 1 cut(s) | 867 |
| BstAUI | TGTACA | 2 cut(s) | 179, 666 |
| BstC8I | GCNNGC | 1 cut(s) | 392 |
| BstDEI | CTNAG | 4 cut(s) | 248, 450, 680, 708 |
| BstF5I | GGATG | 2 cut(s) | 594, 660 |
| BstKTI | GATC | 3 cut(s) | 425, 505, 771 |
| BstMAI | GTCTC | 2 cut(s) | 687, 780 |
| BstMBI | GATC | 3 cut(s) | 422, 502, 768 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 219, 273, 406, 883 |
| BstNI | CCWGG | 1 cut(s) | 9 |
| BstNSI | RCATGY | 3 cut(s) | 267, 276, 520 |
| BstSCI | CCNGG | 1 cut(s) | 7 |
| BstSFI | CTRYAG | 2 cut(s) | 385, 427 |
| BstSLI | GKGCMC | 2 cut(s) | 215, 533 |
| BstV1I | GCAGC | 2 cut(s) | 227, 418 |
| BstV2I | GAAGAC | 2 cut(s) | 592, 622 |
| BstX2I | RGATCY | 1 cut(s) | 768 |
| BstYI | RGATCY | 1 cut(s) | 768 |
| BsuI | GTATCC | 1 cut(s) | 204 |
| BsuRI | GGCC | 1 cut(s) | 222 |
| BtsCI | GGATG | 2 cut(s) | 594, 660 |
| BtsI | GCAGTG | 2 cut(s) | 22, 868 |
| BtsIMutI | CAGTG | 6 cut(s) | 22, 215, 465, 669, 868, 891 |
| BveI | ACCTGC | 1 cut(s) | 405 |
| Cac8I | GCNNGC | 1 cut(s) | 392 |
| Cfr10I | RCCGGY | 1 cut(s) | 345 |
| Csp6I | GTAC | 4 cut(s) | 180, 349, 667, 850 |
| CviAII | CATG | 9 cut(s) | 264, 273, 342, 517, 521, 566, 585, 670, 759 |
| CviQI | GTAC | 4 cut(s) | 180, 349, 667, 850 |
| DdeI | CTNAG | 4 cut(s) | 248, 450, 680, 708 |
| DpnI | GATC | 3 cut(s) | 424, 504, 770 |
| DpnII | GATC | 3 cut(s) | 422, 502, 768 |
| DraIII | CACNNNGTG | 1 cut(s) | 231 |
| EaeI | YGGCCR | 1 cut(s) | 220 |
| Eam1104I | CTCTTC | 1 cut(s) | 867 |
| EarI | CTCTTC | 1 cut(s) | 867 |
| Ecl136II | GAGCTC | 3 cut(s) | 160, 740, 877 |
| Eco24I | GRGCYC | 3 cut(s) | 162, 742, 879 |
| Eco53kI | GAGCTC | 3 cut(s) | 160, 740, 877 |
| Eco88I | CYCGRG | 1 cut(s) | 650 |
| EcoICRI | GAGCTC | 3 cut(s) | 160, 740, 877 |
| EcoRII | CCWGG | 1 cut(s) | 7 |
| EcoT22I | ATGCAT | 1 cut(s) | 522 |
| EcoT38I | GRGCYC | 3 cut(s) | 162, 742, 879 |
| Esp3I | CGTCTC | 1 cut(s) | 687 |
| FaeI | CATG | 9 cut(s) | 267, 276, 345, 520, 524, 569, 588, 673, 762 |
| FatI | CATG | 9 cut(s) | 263, 272, 341, 516, 520, 565, 584, 669, 758 |
| FbaI | TGATCA | 1 cut(s) | 502 |
| Fnu4HI | GCNGC | 3 cut(s) | 241, 277, 407 |
| FokI | GGATG | 2 cut(s) | 601, 667 |
| FriOI | GRGCYC | 3 cut(s) | 162, 742, 879 |
| Fsp4HI | GCNGC | 3 cut(s) | 241, 277, 407 |
| FspBI | CTAG | 2 cut(s) | 35, 834 |
| GluI | GCNGC | 3 cut(s) | 241, 277, 407 |
| HaeIII | GGCC | 1 cut(s) | 222 |
| HapII | CCGG | 2 cut(s) | 173, 346 |
| Hin1II | CATG | 9 cut(s) | 267, 276, 345, 520, 524, 569, 588, 673, 762 |
| HincII | GTYRAC | 2 cut(s) | 487, 514 |
| HindII | GTYRAC | 2 cut(s) | 487, 514 |
| HinfI | GANTC | 4 cut(s) | 38, 326, 822, 888 |
| HpaII | CCGG | 2 cut(s) | 173, 346 |
| HphI | GGTGA | 1 cut(s) | 479 |
| Hpy166II | GTNNAC | 5 cut(s) | 487, 514, 531, 667, 752 |
| Hpy188I | TCNGA | 4 cut(s) | 451, 614, 785, 806 |
| Hpy188III | TCNNGA | 6 cut(s) | 35, 311, 368, 574, 652, 815 |
| Hpy8I | GTNNAC | 5 cut(s) | 487, 514, 531, 667, 752 |
| HpyAV | CCTTC | 1 cut(s) | 310 |
| HpyCH4III | ACNGT | 5 cut(s) | 598, 608, 621, 746, 791 |
| HpyCH4V | TGCA | 6 cut(s) | 20, 243, 256, 414, 520, 531 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 219, 273, 406, 883 |
| HpyF3I | CTNAG | 4 cut(s) | 248, 450, 680, 708 |
| Hsp92II | CATG | 9 cut(s) | 267, 276, 345, 520, 524, 569, 588, 673, 762 |
| KpnI | GGTACC | 1 cut(s) | 352 |
| Ksp22I | TGATCA | 1 cut(s) | 502 |
| Kzo9I | GATC | 3 cut(s) | 422, 502, 768 |
| LguI | GCTCTTC | 1 cut(s) | 867 |
| LmnI | GCTCC | 4 cut(s) | 10, 125, 165, 737 |
| Lsp1109I | GCAGC | 2 cut(s) | 227, 418 |
| LweI | GCATC | 3 cut(s) | 290, 579, 789 |
| MaeI | CTAG | 2 cut(s) | 35, 834 |
| MaeIII | GTNAC | 6 cut(s) | 290, 460, 719, 791, 863, 889 |
| MalI | GATC | 3 cut(s) | 424, 504, 770 |
| MboI | GATC | 3 cut(s) | 422, 502, 768 |
| MboII | GAAGA | 3 cut(s) | 592, 627, 884 |
| MflI | RGATCY | 1 cut(s) | 768 |
| MhlI | GDGCHC | 5 cut(s) | 162, 215, 533, 742, 879 |
| MluCI | AATT | 4 cut(s) | 131, 184, 201, 355 |
| MlyI | GAGTC | 2 cut(s) | 816, 897 |
| MmeI | TCCRAC | 2 cut(s) | 500, 715 |
| MnlI | CCTC | 6 cut(s) | 58, 79, 207, 562, 631, 697 |
| Mph1103I | ATGCAT | 1 cut(s) | 522 |
| MseI | TTAA | 2 cut(s) | 69, 188 |
| MslI | CAYNNNNRTG | 1 cut(s) | 546 |
| MspA1I | CMGCKG | 1 cut(s) | 432 |
| MspI | CCGG | 2 cut(s) | 173, 346 |
| MspR9I | CCNGG | 1 cut(s) | 9 |
| Mva1269I | GAATGC | 1 cut(s) | 583 |
| MvaI | CCWGG | 1 cut(s) | 9 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 219, 273, 406, 883 |
| NdeII | GATC | 3 cut(s) | 422, 502, 768 |
| NlaIII | CATG | 9 cut(s) | 267, 276, 345, 520, 524, 569, 588, 673, 762 |
| NlaIV | GGNNCC | 5 cut(s) | 6, 121, 350, 660, 770 |
| NmuCI | GTSAC | 4 cut(s) | 460, 791, 863, 889 |
| NsiI | ATGCAT | 1 cut(s) | 522 |
| NspI | RCATGY | 3 cut(s) | 267, 276, 520 |
| PciSI | GCTCTTC | 1 cut(s) | 867 |
| PctI | GAATGC | 1 cut(s) | 583 |
| PfeI | GAWTC | 2 cut(s) | 38, 326 |
| PkrI | GCNGC | 3 cut(s) | 242, 278, 408 |
| PleI | GAGTC | 2 cut(s) | 816, 896 |
| PpsI | GAGTC | 2 cut(s) | 816, 896 |
| Psp124BI | GAGCTC | 3 cut(s) | 162, 742, 879 |
| Psp6I | CCWGG | 1 cut(s) | 7 |
| PspGI | CCWGG | 1 cut(s) | 7 |
| PspN4I | GGNNCC | 5 cut(s) | 6, 121, 350, 660, 770 |
| PsuI | RGATCY | 1 cut(s) | 768 |
| PvuII | CAGCTG | 1 cut(s) | 432 |
| RsaI | GTAC | 4 cut(s) | 181, 350, 668, 851 |
| RsaNI | GTAC | 4 cut(s) | 180, 349, 667, 850 |
| RseI | CAYNNNNRTG | 1 cut(s) | 546 |
| SacI | GAGCTC | 3 cut(s) | 162, 742, 879 |
| SapI | GCTCTTC | 1 cut(s) | 867 |
| SaqAI | TTAA | 2 cut(s) | 69, 188 |
| SatI | GCNGC | 3 cut(s) | 241, 277, 407 |
| Sau3AI | GATC | 3 cut(s) | 422, 502, 768 |
| SchI | GAGTC | 2 cut(s) | 816, 897 |
| ScrFI | CCNGG | 1 cut(s) | 9 |
| SduI | GDGCHC | 5 cut(s) | 162, 215, 533, 742, 879 |
| SfaNI | GCATC | 3 cut(s) | 290, 579, 789 |
| SfcI | CTRYAG | 2 cut(s) | 385, 427 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 546 |
| SmlI | CTYRAG | 3 cut(s) | 311, 395, 878 |
| SmoI | CTYRAG | 3 cut(s) | 311, 395, 878 |
| Sse9I | AATT | 4 cut(s) | 131, 184, 201, 355 |
| SsiI | CCGC | 3 cut(s) | 277, 323, 563 |
| SspMI | CTAG | 2 cut(s) | 35, 834 |
| SstI | GAGCTC | 3 cut(s) | 162, 742, 879 |
| StyD4I | CCNGG | 1 cut(s) | 7 |
| TaaI | ACNGT | 5 cut(s) | 598, 608, 621, 746, 791 |
| TaqI | TCGA | 4 cut(s) | 47, 140, 421, 573 |
| TasI | AATT | 4 cut(s) | 131, 184, 201, 355 |
| TatI | WGTACW | 3 cut(s) | 179, 666, 849 |
| TauI | GCSGC | 1 cut(s) | 279 |
| TfiI | GAWTC | 2 cut(s) | 38, 326 |
| Tru1I | TTAA | 2 cut(s) | 69, 188 |
| Tru9I | TTAA | 2 cut(s) | 69, 188 |
| TscAI | CASTG | 6 cut(s) | 22, 215, 465, 669, 868, 898 |
| TseFI | GTSAC | 4 cut(s) | 460, 791, 863, 889 |
| TseI | GCWGC | 2 cut(s) | 240, 406 |
| Tsp45I | GTSAC | 4 cut(s) | 460, 791, 863, 889 |
| TspDTI | ATGAA | 1 cut(s) | 194 |
| TspGWI | ACGGA | 2 cut(s) | 834, 913 |
| TspRI | CASTG | 6 cut(s) | 22, 215, 465, 669, 868, 898 |
| VneI | GTGCAC | 1 cut(s) | 529 |
| XapI | RAATTY | 3 cut(s) | 131, 184, 355 |
| XbaI | TCTAGA | 1 cut(s) | 34 |
| XceI | RCATGY | 3 cut(s) | 267, 276, 520 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 143 |
| XspI | CTAG | 2 cut(s) | 35, 834 |
| Zsp2I | ATGCAT | 1 cut(s) | 522 |