Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001082

Physical Location & Seq

Genomic Coordinates Reverse (-)

6229125 .. 6233025

Gene Structure 3901 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1055100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 864 bp

ATGGCAATCACAAAGTCAGAGACAGGTAGTAATAGTATGCTTAGTAAGACTACCCCTGGTTTGGGTGGAAAGCATGGTCTTTATTCAACCAATGGTGTGCATGAGTTGCTTGAGTGCCCTGTCTGCACGAATTTGATGTACCCTCCTATTCATCAGTGTCCTAATGGCCACACAGTATGTTCGGACTGCAAGATAAGGGTGCACAATTGTTGCCCCACTTGCCGCTATGAGCTTGGTGATATAAGGTGTTTGGCTTTGGAGAAAGTTGCTGAATCATTGGAACTTCCATGCAGATACCAGAGTTTAGGATGTCATGATATTTTTCCTTACTACAGCAAACTCAAACATGAACAACACTGTCGATTTCGTCTATATAATTGCCCTTATGCTGGATCTGAGTGCTCTATTACAGGTGACATCCCCACTCTCGTTGCACATCTTAAAGAGGATCACAAGGTTGACATGCATGATGGGTGCACCTTCAACCATAGATATGTTAAAGCGAACCCAAATGAAGTTGAAAACGCAACTTGGATGCTTACTGTCTTCAATTGTTTCGGAAGACAATTCTGTTTGCACTTTGAGGCTTTCCAGTTAGGAATGGCTCCAGTCTACATGGCCTTTTTACGATTCATGGGTGATGATATTGAGGCTAAGAAATTCAGCTATAGTTTGGAAGTTGGTGCAAATGGCCGTAAGCTAATATGGCAAGGGATCCCTAGGAGTATCCGCGACAGTCATAGAAAAGTCCGTGACAGCCAGGATGGACTCATTATTCAGAGGAATCTGGCTCTCTATTTCTCAGGTGGGGATAGGCAAGAACTAAAGTTGAGGATTACTGGCCGTATATGGAAAGAAGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009894 GO:0009987 GO:0010506 GO:0016241 GO:0016567 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0031323 GO:0031329 GO:0032446 GO:0033043 GO:0036211 GO:0043170 GO:0043412 GO:0044087 GO:0044088 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0050789 GO:0050794 GO:0051128 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1902115 GO:2000785

KEGG Pathways

Metabolic & Signaling

ko04013 ko04115 ko04120 ko04310 map04013 map04115 map04120 map04310

Pfam Domains

Protein Families

Sina

Protein Analysis

288

Amino Acids

32.72

Weight (kDa)

8.09

Isoelectric Point (pI)

38.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sina_RING	PF21362	39 - 74	6.2e-12	E3 ubiquitin-protein ligase sina/sinah, RING finger
Sina_ZnF	PF21361	88 - 163	3.5e-30	Sina, zinc finger
Sina_TRAF	PF03145	183 - 280	1.7e-33	Sina, TRAF-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001082)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41980 AT3G58040 AT3G58040
fragaria_vesca	FvH4_3g44521 FvH4_6g21930
malus_domestica	MD02G1290000.v1.1 MD03G1012000.v1.1 MD07G1037000.v1.1 MD11G1014900.v1.1 MD12G1055100.v1.1 MD14G1054300.v1.1
prunus_persica	Prupe.2G032500_v2.0.a1 Prupe.2G032500_v2.0.a1 Prupe.2G032500_v2.0.a1 Prupe.2G032500_v2.0.a1 Prupe.2G032500_v2.0.a1 Prupe.6G012400_v2.0.a1 Prupe.6G012400_v2.0.a1 Prupe.6G012400_v2.0.a1 Prupe.6G012400_v2.0.a1 Prupe.6G012400_v2.0.a1 Prupe.6G012400_v2.0.a1 Prupe.7G080800_v2.0.a1 Prupe.7G080800_v2.0.a1 Prupe.7G080800_v2.0.a1 Prupe.7G080800_v2.0.a1 Prupe.7G080800_v2.0.a1
pyrus_communis	pycom02g24550 pycom03g01070 pycom07g02580 pycom12g04810 pycom14g04480
rosa_chinensis	RchiOBHm_Chr1g0325251 RchiOBHm_Chr3g0479261 RchiOBHm_Chr5g0081221
rosa_laevigata	RLG00000023589 RLG00000036920
rosa_multiflora	Rmu_sc0002978.1_g000005 Rmu_sc0007768.1_g000002 Rmu_sc0036084.1_g000002 Rmu_ssc0000432.1_g000070
rosa_roxburghii	Rroxscaffold_1G00002250 Rroxscaffold_6G00402480
rosa_rugosa	Rorug03G0173400 Rorug03G0173500 Rorug03G0173600 Rorug05G0474800 Rorug05G0475000 Rorug05G0475000
rosa_samantha	Rh3AG224400 Rh3AG224900 Rh3BG257400 Rh3DG249200 Rh5AG526600 Rh5BG550800 Rh5CG573700 Rh5DG560900
rosa_wichuraiana	Rw3G020110 Rw5G048950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	612
AccII	CGCG	1 cut(s)	732
AciI	CCGC	2 cut(s)	223, 730
AclWI	GGATC	4 cut(s)	400, 456, 709, 722
AcoI	YGGCCR	3 cut(s)	166, 691, 841
AcsI	RAATTY	2 cut(s)	130, 659
AfaI	GTAC	1 cut(s)	140
AfiI	CCNNNNNNNGG	3 cut(s)	61, 62, 389
AgsI	TTSAA	4 cut(s)	87, 484, 521, 550
AjnI	CCWGG	2 cut(s)	55, 759
AluBI	AGCT	3 cut(s)	232, 666, 700
AluI	AGCT	3 cut(s)	232, 666, 700
Alw21I	GWGCWC	3 cut(s)	204, 404, 479
Alw26I	GTCTC	1 cut(s)	14
Alw44I	GTGCAC	2 cut(s)	200, 475
AlwI	GGATC	4 cut(s)	400, 456, 709, 722
AoxI	GGCC	4 cut(s)	166, 618, 691, 841
ApaLI	GTGCAC	2 cut(s)	200, 475
ApoI	RAATTY	2 cut(s)	130, 659
AspA2I	CCTAGG	1 cut(s)	719
AsuHPI	GGTGA	3 cut(s)	248, 425, 650
AvrII	CCTAGG	1 cut(s)	719
BaeGI	GKGCMC	3 cut(s)	119, 204, 479
BaeI	ACNNNNGTAYC	2 cut(s)	286, 319
BalI	TGGCCA	1 cut(s)	168
BamHI	GGATCC	1 cut(s)	714
BbsI	GAAGAC	2 cut(s)	538, 568
Bbv12I	GWGCWC	3 cut(s)	204, 404, 479
BccI	CCATC	2 cut(s)	464, 758
BceAI	ACGGC	2 cut(s)	678, 828
BciT130I	CCWGG	2 cut(s)	57, 761
BciVI	GTATCC	1 cut(s)	737
BcoDI	GTCTC	1 cut(s)	14
BfaI	CTAG	1 cut(s)	720
BfmI	CTRYAG	2 cut(s)	331, 667
BfuI	GTATCC	1 cut(s)	737
BisI	GCNGC	1 cut(s)	223
BlnI	CCTAGG	1 cut(s)	719
BlsI	GCNGC	1 cut(s)	224
Bme1390I	CCNGG	2 cut(s)	57, 761
BmiI	GGNNCC	2 cut(s)	606, 716
BmrFI	CCNGG	2 cut(s)	57, 761
BmsI	GCATC	1 cut(s)	525
BpiI	GAAGAC	2 cut(s)	538, 568
BpmI	CTGGAG	1 cut(s)	591
BpuEI	CTTGAG	1 cut(s)	131
BsaJI	CCNNGG	2 cut(s)	55, 719
Bsc4I	CCNNNNNNNGG	3 cut(s)	61, 62, 389
Bse1I	ACTGG	3 cut(s)	592, 608, 844
BseBI	CCWGG	2 cut(s)	57, 761
BseDI	CCNNGG	2 cut(s)	55, 719
BseGI	GGATG	4 cut(s)	314, 417, 540, 769
BseLI	CCNNNNNNNGG	3 cut(s)	61, 62, 389
BseMII	CTCAG	2 cut(s)	387, 816
BseNI	ACTGG	3 cut(s)	592, 608, 844
BseSI	GKGCMC	3 cut(s)	119, 204, 479
BsgI	GTGCAG	1 cut(s)	109
Bsh1236I	CGCG	1 cut(s)	732
BshFI	GGCC	4 cut(s)	168, 620, 693, 843
BsiHKAI	GWGCWC	3 cut(s)	204, 404, 479
BslI	CCNNNNNNNGG	3 cut(s)	61, 62, 389
BsmAI	GTCTC	1 cut(s)	14
BsnI	GGCC	4 cut(s)	168, 620, 693, 843
Bsp1286I	GDGCHC	4 cut(s)	119, 204, 404, 479
Bsp143I	GATC	3 cut(s)	392, 448, 714
BspACI	CCGC	2 cut(s)	223, 730
BspANI	GGCC	4 cut(s)	168, 620, 693, 843
BspCNI	CTCAG	2 cut(s)	388, 815
BspFNI	CGCG	1 cut(s)	732
BspHI	TCATGA	1 cut(s)	313
BspLI	GGNNCC	2 cut(s)	606, 716
BspPI	GGATC	4 cut(s)	400, 456, 709, 722
BsrI	ACTGG	3 cut(s)	592, 608, 844
BssECI	CCNNGG	2 cut(s)	55, 719
BssMI	GATC	3 cut(s)	392, 448, 714
BssT1I	CCWWGG	1 cut(s)	719
Bst2UI	CCWGG	2 cut(s)	57, 761
Bst4CI	ACNGT	4 cut(s)	175, 359, 544, 737
BstAPI	GCANNNNNTGC	1 cut(s)	106
BstDEI	CTNAG	4 cut(s)	41, 396, 654, 802
BstF5I	GGATG	4 cut(s)	314, 417, 540, 769
BstFNI	CGCG	1 cut(s)	732
BstKTI	GATC	3 cut(s)	395, 451, 717
BstMAI	GTCTC	1 cut(s)	14
BstMBI	GATC	3 cut(s)	392, 448, 714
BstMWI	GCNNNNNNNGC	4 cut(s)	106, 123, 219, 706
BstNI	CCWGG	2 cut(s)	57, 761
BstNSI	RCATGY	1 cut(s)	466
BstSCI	CCNGG	2 cut(s)	55, 759
BstSFI	CTRYAG	2 cut(s)	331, 667
BstSLI	GKGCMC	3 cut(s)	119, 204, 479
BstUI	CGCG	1 cut(s)	732
BstV2I	GAAGAC	2 cut(s)	538, 568
BstX2I	RGATCY	2 cut(s)	392, 714
BstYI	RGATCY	2 cut(s)	392, 714
BsuI	GTATCC	1 cut(s)	737
BsuRI	GGCC	4 cut(s)	168, 620, 693, 843
BtsCI	GGATG	4 cut(s)	314, 417, 540, 769
BtsIMutI	CAGTG	2 cut(s)	161, 355
CciI	TCATGA	1 cut(s)	313
Csp6I	GTAC	1 cut(s)	139
CspCI	CAANNNNNGTGG	2 cut(s)	412, 447
CviAII	CATG	9 cut(s)	74, 101, 288, 314, 347, 463, 467, 616, 634
CviQI	GTAC	1 cut(s)	139
DdeI	CTNAG	4 cut(s)	41, 396, 654, 802
DpnI	GATC	3 cut(s)	394, 450, 716
DpnII	GATC	3 cut(s)	392, 448, 714
EaeI	YGGCCR	3 cut(s)	166, 691, 841
Eco130I	CCWWGG	1 cut(s)	719
EcoRII	CCWGG	2 cut(s)	55, 759
EcoT14I	CCWWGG	1 cut(s)	719
EcoT22I	ATGCAT	1 cut(s)	468
ErhI	CCWWGG	1 cut(s)	719
FaeI	CATG	9 cut(s)	77, 104, 291, 317, 350, 466, 470, 619, 637
FatI	CATG	9 cut(s)	73, 100, 287, 313, 346, 462, 466, 615, 633
FblI	GTMKAC	1 cut(s)	612
Fnu4HI	GCNGC	1 cut(s)	223
FokI	GGATG	4 cut(s)	321, 404, 547, 776
Fsp4HI	GCNGC	1 cut(s)	223
FspBI	CTAG	1 cut(s)	720
GluI	GCNGC	1 cut(s)	223
GsuI	CTGGAG	1 cut(s)	591
HaeIII	GGCC	4 cut(s)	168, 620, 693, 843
Hin1II	CATG	9 cut(s)	77, 104, 291, 317, 350, 466, 470, 619, 637
HincII	GTYRAC	1 cut(s)	460
HindII	GTYRAC	1 cut(s)	460
HinfI	GANTC	4 cut(s)	272, 630, 768, 784
HphI	GGTGA	3 cut(s)	248, 425, 650
Hpy166II	GTNNAC	4 cut(s)	202, 460, 477, 613
Hpy188I	TCNGA	5 cut(s)	19, 184, 397, 560, 780
Hpy188III	TCNNGA	1 cut(s)	314
Hpy8I	GTNNAC	4 cut(s)	202, 460, 477, 613
HpyAV	CCTTC	1 cut(s)	490
HpyCH4III	ACNGT	4 cut(s)	175, 359, 544, 737
HpyF10VI	GCNNNNNNNGC	4 cut(s)	106, 123, 219, 706
HpyF3I	CTNAG	4 cut(s)	41, 396, 654, 802
Hsp92II	CATG	9 cut(s)	77, 104, 291, 317, 350, 466, 470, 619, 637
Kzo9I	GATC	3 cut(s)	392, 448, 714
LmnI	GCTCC	1 cut(s)	610
LweI	GCATC	1 cut(s)	525
MaeI	CTAG	1 cut(s)	720
MaeIII	GTNAC	2 cut(s)	413, 752
MalI	GATC	3 cut(s)	394, 450, 716
MboI	GATC	3 cut(s)	392, 448, 714
MboII	GAAGA	2 cut(s)	538, 573
MfeI	CAATTG	2 cut(s)	205, 550
MflI	RGATCY	2 cut(s)	392, 714
MhlI	GDGCHC	4 cut(s)	119, 204, 404, 479
MlsI	TGGCCA	1 cut(s)	168
MluCI	AATT	6 cut(s)	130, 205, 376, 550, 566, 659
MluNI	TGGCCA	1 cut(s)	168
MlyI	GAGTC	1 cut(s)	762
MnlI	CCTC	6 cut(s)	153, 439, 577, 643, 774, 825
Mox20I	TGGCCA	1 cut(s)	168
Mph1103I	ATGCAT	1 cut(s)	468
MscI	TGGCCA	1 cut(s)	168
MseI	TTAA	2 cut(s)	441, 498
MslI	CAYNNNNRTG	1 cut(s)	492
Msp20I	TGGCCA	1 cut(s)	168
MspR9I	CCNGG	2 cut(s)	57, 761
MunI	CAATTG	2 cut(s)	205, 550
MvaI	CCWGG	2 cut(s)	57, 761
MvnI	CGCG	1 cut(s)	732
MwoI	GCNNNNNNNGC	4 cut(s)	106, 123, 219, 706
NdeII	GATC	3 cut(s)	392, 448, 714
NlaIII	CATG	9 cut(s)	77, 104, 291, 317, 350, 466, 470, 619, 637
NlaIV	GGNNCC	2 cut(s)	606, 716
NmuCI	GTSAC	2 cut(s)	413, 752
NsiI	ATGCAT	1 cut(s)	468
NspI	RCATGY	1 cut(s)	466
PagI	TCATGA	1 cut(s)	313
PfeI	GAWTC	3 cut(s)	272, 630, 784
PkrI	GCNGC	1 cut(s)	224
PleI	GAGTC	1 cut(s)	762
PpsI	GAGTC	1 cut(s)	762
Psp6I	CCWGG	2 cut(s)	55, 759
PspGI	CCWGG	2 cut(s)	55, 759
PspN4I	GGNNCC	2 cut(s)	606, 716
PsuI	RGATCY	2 cut(s)	392, 714
RsaI	GTAC	1 cut(s)	140
RsaNI	GTAC	1 cut(s)	139
RseI	CAYNNNNRTG	1 cut(s)	492
SaqAI	TTAA	2 cut(s)	441, 498
SatI	GCNGC	1 cut(s)	223
Sau3AI	GATC	3 cut(s)	392, 448, 714
SchI	GAGTC	1 cut(s)	762
ScrFI	CCNGG	2 cut(s)	57, 761
SduI	GDGCHC	4 cut(s)	119, 204, 404, 479
SetI	ASST	9 cut(s)	28, 234, 248, 415, 459, 482, 668, 702, 808
SfaNI	GCATC	1 cut(s)	525
SfcI	CTRYAG	2 cut(s)	331, 667
SmiMI	CAYNNNNRTG	1 cut(s)	492
SmlI	CTYRAG	1 cut(s)	110
SmoI	CTYRAG	1 cut(s)	110
Sse9I	AATT	6 cut(s)	130, 205, 376, 550, 566, 659
SsiI	CCGC	2 cut(s)	223, 730
SspMI	CTAG	1 cut(s)	720
StyD4I	CCNGG	2 cut(s)	55, 759
StyI	CCWWGG	1 cut(s)	719
TaaI	ACNGT	4 cut(s)	175, 359, 544, 737
TaqI	TCGA	1 cut(s)	361
TasI	AATT	6 cut(s)	130, 205, 376, 550, 566, 659
TauI	GCSGC	1 cut(s)	225
TfiI	GAWTC	3 cut(s)	272, 630, 784
Tru1I	TTAA	2 cut(s)	441, 498
Tru9I	TTAA	2 cut(s)	441, 498
TscAI	CASTG	2 cut(s)	161, 362
TseFI	GTSAC	2 cut(s)	413, 752
Tsp45I	GTSAC	2 cut(s)	413, 752
TspDTI	ATGAA	4 cut(s)	140, 363, 528, 622
TspGWI	ACGGA	1 cut(s)	740
TspRI	CASTG	2 cut(s)	161, 362
VneI	GTGCAC	2 cut(s)	200, 475
XapI	RAATTY	2 cut(s)	130, 659
XceI	RCATGY	1 cut(s)	466
XmaJI	CCTAGG	1 cut(s)	719
XmiI	GTMKAC	1 cut(s)	612
XspI	CTAG	1 cut(s)	720
Zsp2I	ATGCAT	1 cut(s)	468

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.